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https://www.readbyqxmd.com/read/29057890/effect-of-nitrate-leaching-caused-by-swine-manure-application-in-fields-of-the-yellow-river-irrigation-zone-of-ningxia-china
#1
Shiqi Yang, Yongsheng Wang, Ruliang Liu, Aiping Zhang, Zhengli Yang
A five-year swine manure application trial and a study of nitrate leaching losses have been conducted. There were three treatments: traditional without manure (CK), traditional matched manure 4500 kg ha(-2) (T1) and traditional matched manure 9000 kg ha(-2) (T2). Nitrate nitrogen leaching losses at the 30-, 60-, and 90-cm soil layers were measured using the resin core method. The results indicate that the swine manure application did not noticeably increase soil nitrate leaching losses in the 30-cm layer...
October 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29057836/citrulline-malate-does-not-improve-muscle-recovery-after-resistance-exercise-in-untrained-young-adult-men
#2
Douglas K da Silva, Jeferson L Jacinto, Walquiria B de Andrade, Mirela C Roveratti, José M Estoche, Mario C W Balvedi, Douglas B de Oliveira, Rubens A da Silva, Andreo F Aguiar
The effects of citrulline malate (CM) on muscle recovery from resistance exercise remains unknown. We aimed to determine if citrulline malate supplementation improves muscle recovery after a single session of high-intensity resistance exercise (RE) in untrained young adult men. Nine young adult men (24.0 ± 3.3 years) participated in a double-blind crossover study in which they received 6 g of CM and placebo (PL) on two occasions, separated by a seven-day washout period. Each occasion consisted of a single session of high-intensity RE (0 h) and three subsequent fatigue tests sessions (at 24, 48, and 72 h) to assess the time course of muscle recovery...
October 18, 2017: Nutrients
https://www.readbyqxmd.com/read/29056268/successful-treatment-of-a-patient-with-statin-induced-myopathy-and-myotonic-dystrophy-type-ii-with-proprotein-convertase-subtilisin-kexin-type-9-inhibitor-alirocumab-praluent
#3
Mohamed K M Shakir, Terry Shin, Thanh D Hoang, Vinh Q Mai
Presently there are limited treatment options for hypercholesterolemia in patients with statin intolerance and myotonic dystrophy. A 74 year-old male presented to endocrine clinic with hypercholesterolemia (serum LDL-C 210 mg/dL), hypogonadism, insulin-controlled type 2 diabetes mellitus, and minimally elevated serum creatine kinase (CK) levels (184 U/L, ref. range 38-174). Shortly after simvastatin treatment, patient developed severe myalgias in the proximal lower and upper extremities; and serum CK increased to 317 U/L...
September 4, 2017: Journal of Clinical Lipidology
https://www.readbyqxmd.com/read/29053816/histomorphometric-study-of-the-anterior-latissimus-dorsi-muscle-and-evaluation-of-enzymatic-markers-of-broilers-affected-with-dorsal-cranial-myopathy
#4
R Sesterhenn, F M Siqueira, A C Hamerski, D Driemeier, S F Valle, S L Vieira, L Kindlein, V P Nascimento
Dorsal cranial myopathy (DCM), which affects the anterior latissimus dorsi (ALD) muscles of commercial broilers, is of unknown etiology, and it represents up to 6% of the partial condemnations in Brazilian slaughterhouses. This study was performed to achieve histomorphometric characterizations of the ALD muscles from male Cobb 500 broilers slaughtered at either 35 d or 42 d and to evaluate the effects of DCM on the enzymatic markers aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine kinase (CK), and lactate dehydrogenase (LDH) and on uric acid and creatinine metabolites...
September 20, 2017: Poultry Science
https://www.readbyqxmd.com/read/29052788/sequence-analysis-of-six-full-length-bean-yellow-mosaic-virus-genomes-reveals-phylogenetic-diversity-in-india-strains-suggesting-subdivision-of-phylogenetic-group-iv
#5
Charanjeet Kaur, Rashmi Raj, Ashish Srivastava, Susheel Kumar, Shri Krishna Raj
We report the complete genome sequence of five bean yellow mosaic virus (BYMV) isolates (CK-GL1, CK-GL3, CK-GL4, CK-GL5 and Vfaba2) that share 74.6-98.9% (nucleotide) and 81.5-99.1% (amino acid) identity with globally available BYMV sequences. Phylogenetic analysis clustered them specifically in BYMV phylogenetic group-IV within the existing nine groups. The CK-GL1, CK-GL2, CK-GL4 and CK-GL5 isolates formed a discrete cluster within group-IV. The present study suggests subdivision of group-IV into subgroup-IVa and IVb...
October 20, 2017: Archives of Virology
https://www.readbyqxmd.com/read/29051614/genome-wide-identification-of-micrornas-responsive-to-ectropis-oblique-feeding-in-tea-plant-camellia-sinensis-l
#6
Anburaj Jeyaraj, Shengrui Liu, Xiao Zhang, Ran Zhang, Mingzhu Shangguan, Chaoling Wei
The tea plant (Camellia sinensis L.) is vulnerable to the geometrid Ectropis oblique; although microRNAs (miRNAs) are important for plant growth, development and stress response, the function of miRNAs in the response of C. sinensis to stress from E. oblique is unclear. To identify E. oblique stress-responsive miRNAs and their target genes in tea plant, three small RNA libraries were constructed from leaves subjected to mechanical wounding (MW), geometrid attack (GA) and from healthy control (CK) leaves. Using high-throughput sequencing, 130 known miRNAs and 512 novel miRNAs were identified; of these, differential expression under GA stress was observed for 36 known and 139 novel miRNAs...
October 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29050412/adaptive-response-to-exercise-of-fast-growing-and-slow-growing-chicken-strains-blood-oxidative-status-and-non-enzymatic-antioxidant-defense
#7
S Mattioli, A Dal Bosco, S Ruggeri, M Martino, L Moscati, C Pesca, C Castellini
The adaptation of chickens to free-range rearing systems mainly involves the locomotory behavior, which is very different in fast-growing (FG) and slow-growing (SG) strains. This study aimed to compare the effect of moderate locomotory activity (induced and prolonged) on the blood oxidative status in a slow-growing chicken strain with that in a fast-growing one. Thirty FG (Ross 308) birds and 30 SG (Hubbard) birds were divided into 2 groups for each strain and subjected to different treatments: no exercise (the control group [C]) and 1 h of walking at 4 km/h (the exercise group [E])...
September 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/29050295/a-comprehensive-study-of-circulating-tumour-cells-at-the-moment-of-prostate-cancer-diagnosis-biological-and-clinical-implications-of-egfr-ar-and-snps
#8
Ignacio Puche-Sanz, María J Alvarez-Cubero, Manrique Pascual-Geler, Alba Rodríguez-Martínez, Miguel Delgado-Rodríguez, José L García-Puche, José Expósito, Inmaculada Robles-Fernández, Carmen Entrala-Bernal, José A Lorente, José M Cózar-Olmo, María J Serrano
Circulating tumor cells (CTCs) have been recently accepted as prognostic markers in metastatic prostate cancer (PCa). However, very few studies have analyzed their role in early-stage PCa. The aim of this research is to study the value of CTCs at the moment of PCa diagnosis and to identify different subpopulations of CTCs. Patients with PSA value > 4 ng/ml and clinical suspicion of PCa were included. Samples were collected immediately before prostatic biopsy. CTCs were isolated by immunomagnetic technique using a multi-CK specific antibody...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29049733/tunneling-nanotubes-are-novel-cellular-structures-that-communicate-signals-between-trabecular-meshwork-cells
#9
Kate E Keller, John M Bradley, Ying Ying Sun, Yong-Feng Yang, Ted S Acott
Purpose: The actin cytoskeleton of trabecular meshwork (TM) cells plays a role in regulating aqueous humor outflow. Many studies have investigated stress fibers, but F-actin also assembles into other supramolecular structures including filopodia. Recently, specialized filopodia called tunneling nanotubes (TNTs) have been described, which communicate molecular signals and organelles directly between cells. Here, we investigate TNT formation by TM cells. Methods: Human TM cells were labeled separately with the fluorescent dyes, DiO and DiD, or with mitochondrial dye...
October 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29048728/cytokeratin7-expression-in-histologic-and-cytologic-specimens-of-cystic-neck-metastasis-from-hpv-positive-squamous-cell-carcinoma-of-the-tonsil-a-case-report
#10
Yong Kyun Cho, Seung Ju Lee, Jeong Joo Woo, Hyekyung Lee, Hojung Lee
The majority of cystic squamous cell carcinomas (SCCs) of the neck have been shown to be metastatic tumors from tonsillar SCCs associated with high-risk human papillomavirus (HR HPV). Recent studies have demonstrated cytokeratin (CK)7 involvement in the development of HPV positive SCC, but no report has been issued on its simultaneous expression in primary tonsillar and metastatic tumor with cystic change. We present a case of HPV positive tonsillar SCC of a 42-year-old male that initially manifested as a cystic neck mass expressing CK7, CK19, and p16 in primary and metastatic tumors...
October 19, 2017: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/29047154/effects-of-testosterone-enanthate-and-resistance-training-on-myocardium-in-wistar-rats-clinical-and-anatomical-pathology
#11
S Karbasi, M Zaeemi, M Mohri, A Rashidlamir, Z Moosavi
This study was performed to determine the effects of 8 weeks testosterone enanthate (TE) injection and resistance training (RT) on cardiac muscle in male Wistar rats. A total of 28 male adult Wistar rats were randomly divided into 4 groups; control + placebo, RT + placebo, TE and TE + RT. Testosterone enanthate (20 mg/kg BW, IM) and placebo (olive oil; 0.2 ml, IM) were injected twice a week for 2 months. The RT consisted of climbing (5 reps/3 sets) a ladder carrying a load suspended from the tail...
October 18, 2017: Andrologia
https://www.readbyqxmd.com/read/29046420/della1-gibberellin-signaling-regulates-cytokinin-dependent-symbiotic-nodulation
#12
Camille Fonouni-Farde, Anna Kisiala, Mathias Brault, R J Neil Emery, Anouck Diet, Florian Frugier
In legume plants, low nitrogen soils promote symbiotic interactions with rhizobial bacteria, leading to the formation of nitrogen-fixing root nodules. Amongst critical signals regulating this developmental process are bacterial Nod Factors (NFs) and several plant hormones, including cytokinins (CKs) and gibberellins (GAs). Here we show in Medicago truncatula that GA signaling mediated by DELLA1 decreases the amount of bioactive CKs in roots and negatively impacts the CRE1 (Cytokinin Response 1)-dependent NF activation of a subset of CK-signaling genes, as well as of the CK-regulated NSP2 (Nodulation Signaling pathway 2) and ERN1 (ERF required for Nodulation) early nodulation genes...
October 18, 2017: Plant Physiology
https://www.readbyqxmd.com/read/29045727/short-term-thermal-photosynthetic-responses-of-c4-grasses-are-independent-of-the-biochemical-subtype
#13
Balasaheb V Sonawane, Robert E Sharwood, Susanne von Caemmerer, Spencer M Whitney, Oula Ghannoum, Christine Raines
C4 photosynthesis evolved independently many times, resulting in multiple biochemical pathways; however, little is known about how these different pathways respond to temperature. We investigated the photosynthetic responses of eight C4 grasses belonging to three biochemical subtypes (NADP-ME, PEP-CK, and NAD-ME) to four leaf temperatures (18, 25, 32, and 40 °C). We also explored whether the biochemical subtype influences the thermal responses of (i) in vitro PEPC (Vpmax) and Rubisco (Vcmax) maximal activities, (ii) initial slope (IS) and CO2-saturated rate (CSR) derived from the A-Ci curves, and (iii) CO2 leakage out of the bundle sheath estimated from carbon isotope discrimination...
October 16, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29042932/cardioprotective-effect-of-notch-signaling-on-the-development-of-myocardial-infarction-complicated-by-diabetes-mellitus
#14
Fang Wu, Bo Yu, Xu Zhang, Yuelan Zhang
The present study aimed to elucidate the role of Notch signaling in the development of myocardial infarction (MI) concomitant with diabetes in vivo and in vitro and evaluated the therapeutic effect of the Notch signaling in vitro. Streptozotocin-induced diabetic rats were subjected to 25 min of ischemia and 2 h of reperfusion. Cardiac troponin T (cTnT) and creatine kinase-MB (CK-MB) isoenzyme levels were detected. Infarct size was measured by 2,3,5-triphenyltetrazolium chloride staining. Myocardial apoptosis and fibrosis were examined by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling and Masson Trichrome staining, respectively...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29042679/municipal-sewage-sludge-compost-promotes-mangifera-persiciforma-tree-growth-with-no-risk-of-heavy-metal-contamination-of-soil
#15
Shuangshuang Chu, Daoming Wu, Liyin L Liang, Fengdi Zhong, Yaping Hu, Xinsheng Hu, Can Lai, Shucai Zeng
Application of sewage sludge compost (SSC) as a fertilizer on landscaping provides a potential way for the effective disposal of sludge. However, the response of landscape trees to SSC application and the impacts of heavy metals from SSC on soil are poorly understood. We conducted a pot experiment to investigate the effects of SSC addition on Mangifera persiciforma growth and quantified its uptake of heavy metals from SSC by setting five treatments with mass ratios of SSC to lateritic soil as 0%:100% (CK), 15%:85% (S15), 30%:70% (S30), 60%:40% (S60), and 100%:0% (S100)...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29040984/ability-of-cytokeratin-18-fragments-and-fib-4-index-to-diagnose-overall-and-mild-fibrosis-nonalcoholic-steatohepatitis-in-japanese-nonalcoholic-fatty-liver-disease-patients
#16
Natsuko Kobayashi, Takashi Kumada, Hidenori Toyoda, Toshifumi Tada, Takanori Ito, Masayoshi Kage, Takeshi Okanoue, Masatoshi Kudo
BACKGROUND: Several laboratory markers used in lieu of liver biopsy are reportedly useful in the diagnosis of nonalcoholic steatohepatitis (NASH). In the present study, we investigated the diagnostic impact of various non-invasive markers for predicting NASH. METHODS: A total of 229 nonalcoholic fatty liver disease (NAFLD) patients who underwent liver biopsy were enrolled for the study. The diagnostic ability of various markers to diagnose NASH from NAFLD was investigated...
2017: Digestive Diseases
https://www.readbyqxmd.com/read/29039314/effects-of-puerarin-on-lipopolysaccharide-induced-myocardial-dysfunction-in-isolated-rat-hearts
#17
Zhang Shaojun, Xu Yanyan, Chen Jian, Zheng Xia, Fang Qiang, Jiang Saiping
Myocardial dysfunction is a serious complication induced by sepsis. Puerarin is an oriental medicine that possesses therapeutic benefits for cardiovascular diseases. The aim of this study was to evaluate the anti-myocardial dysfunction effects of puerarin in isolated rat hearts induced by lipopolysaccharide- and compare the myocardial protective effects between the different concentrations of puerarin. Isolated hearts were attached to a Langendorff apparatus and perfused with lipopolysaccharide (LPS) and different concentrations of puerarin...
July 2017: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29039114/long-term-survival-after-repeated-resection-of-metachronous-lung-metastases-from-pstage-ia-pancreatic-adenocarcinoma
#18
Mamoru Miyasaka, Takehiro Noji, Kazuto Ohtaka, Ryohei Chiba, Shoki Sato, Yasuhito Shoji, Ryunosuke Hase, Tatsunosuke Ichimura, Satoshi Hirano, Naoto Senmaru
CASE REPORT: A 70-year-old woman with pancreatic ductal adenocarcinoma was initially treated by distal pancreatectomy (DP). Thirty-five months later, another tumor appeared in the pancreatic head and was treated by pancreaticoduodenectomy. Histopathological findings identified both tumors as pancreatic ductal adenocarcinoma pStage IA. Computed tomography (CT) of the chest 16 months after the second pancreatectomy revealed a ground-glass opacity in segment 3 of the right lung. Chest CT 23 months after the second pancreatectomy revealed a nodular shadow in segment 1a of the right lung...
October 16, 2017: Clinical Journal of Gastroenterology
https://www.readbyqxmd.com/read/29038020/the-ca-2-camkk2-axis-mediates-the-telbivudine-induces-upregulation-of-creatine-kinase-implications-for-mechanism-of-antiviral-nucleoside-analogs-side-effect
#19
Long Jianfei, Wang Min, Ma Chunlai, Chen Bicui, Zhang Jiming, Wang Bin
Telbivudine (LdT), a widely prescribed anti-hepatitis B virus (HBV) drug for the treatment of chronic Hepatitis B (CHB), causes adverse reactions ranging from creatine kinase (CK) elevation to myopathy. The purpose of this study was to explore the mechanism(s) of LdT induced CK elevation. The effects of LdT on mitochondrial morphology and proteins (TK2 and β-actin), oxidative stress, intracellular Ca(2+) levels, Ca(2+)-related signaling pathway (CaMKK2/AMPK), and Ca(2+)-related biomarkers such as superoxide dismutase (SOD) and malondialdehyde (MDA) were assessed in human skeletal muscle cells (HSKMCs)...
October 13, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/29037855/nephrosphere-derived-cells-are-induced-to-multilineage-differentiation-when-cultured-on-human-decellularized-kidney-scaffolds
#20
Silvia Bombelli, Chiara Meregalli, Carla Scalia, Giorgio Bovo, Barbara Torsello, Sofia De Marco, Massimiliano Cadamuro, Paolo Viganò, Guido Strada, Giorgio Cattoretti, Cristina Bianchi, Roberto A Perego
In end-stage chronic kidney disease, the option of organ transplantation is limited due to the scarce availability of kidneys. The combination of stem cell research, regenerative medicine, and tissue engineering seems a promising approach to produce new transplantable kidneys. Currently, the possibility to repopulate naturally obtained scaffolds with cells of different sources is advancing. Our aim was to test, for the first time, whether the nephrosphere (NS) cells, composed by renal stem/progenitor-like cells, were able to repopulate different nephron portions of renal extracellular matrix scaffolds obtained after decellularization of human renal tissue slices...
October 13, 2017: American Journal of Pathology
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