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https://www.readbyqxmd.com/read/29621294/the-redox-sensing-protein-rex-modulates-ethanol-production-in-thermoanaerobacterium-saccharolyticum
#1
Tianyong Zheng, Anthony A Lanahan, Lee R Lynd, Daniel G Olson
Thermoanaerobacterium saccharolyticum is a thermophilic anaerobe that has been engineered to produce high amounts of ethanol, reaching ~90% theoretical yield at a titer of 70 g/L. Here we report the physiological changes that occur upon deleting the redox-sensing transcriptional regulator Rex in wild type T. saccharolyticum: a single deletion of rex resulted in a two-fold increase in ethanol yield (from 40% to 91% theoretical yield), but the resulting strains grew only about a third as fast as the wild type strain...
2018: PloS One
https://www.readbyqxmd.com/read/29344698/concentrations-of-cadmium-copper-and-zinc-in-macrobrachium-rosenbergii-giant-freshwater-prawn-from-natural-environment
#2
Farah Akmal Idrus, Masania Mohd Basri, Khairul Adha A Rahim, Nur Syazwani Abd Rahim, Melissa Dennis Chong
This study analyzed the levels of cadmium (Cd), copper (Cu), and zinc (Zn) by the flame atomic absorption spectrophotometer (FAAS), in the muscle tissues, exoskeletons, and gills from freshwater prawn (Macrobrachium rosenbergii) (n = 20) harvested from natural habitat in Kerang River, Malaysia on 25th November 2015. Significant increase of the metals level in muscle tissue and gill (r > 0.70, p < 0.05) were observed with increase in length except for Cu in gills. No relationship was found between metals level in exoskeleton and length...
January 17, 2018: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/29344592/ph-responsible-fluorescent-carbon-nanoparticles-for-tumor-selective-theranostics-via-ph-turn-on-off-fluorescence-and-photothermal-effect-in-vivo-and-in-vitro
#3
Eun Bi Kang, Jung Eun Lee, Zihnil Adha Islamy Mazrad, Insik In, Ji Hoon Jeong, Sung Young Park
We developed nanoparticles comprising a photothermal dye (IR825)-loaded carbonized zwitterionic polymer [FNP-I] as "switch-on" pH-responsive fluorescence probes to sense intracellular cancer cells and for near-infrared (NIR) controllable photothermal therapy (PTT) in vivo and in vitro. The fluorescent "off" of FNP-I was activated after reaching the cancer cell environment, where the zwitterionic compartment of FNP lost its hydrophobicity to induce PTT-mediated heat release of IR825 under NIR irradiation in the tumor...
January 18, 2018: Nanoscale
https://www.readbyqxmd.com/read/29239423/two-step-synthesis-and-characterization-of-vertically-stacked-sns-ws-2-and-sns-mos-2-p-n-heterojunctions
#4
Adha Sukma Aji, Masanori Izumoto, Kenshiro Suenaga, Keisuke Yamamoto, Hiroshi Nakashima, Hiroki Ago
We demonstrate the synthesis of unique heterostructures consisting of SnS and WS2 (or SnS and MoS2 ) by two-step chemical vapor deposition (CVD). After the first CVD growth of triangular WS2 (MoS2 ) grains, the second CVD step was performed to grow square SnS grains on the same substrate. We found that these SnS grains can be grown at very low temperature with the substrate temperature of 200 °C. Most of the SnS grains nucleated from the side edges of WS2 (MoS2 ) grains, resulting in the formation of partly stacked heterostructures with a large overlapping area...
January 3, 2018: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29238193/in-vivo-evaluation-of-a-novel-nanocomposite-porous-3d-scaffold-in-a-rabbit-model-histological-analysis
#5
Saffanah Khuder Mahmood, Intan-Shameha Abdul Razak, Mustafa Saddam Ghaji, Loqman Mohamed Yusof, Zaid Khudhur Mahmood, Mohd Adha Bin P Rameli, Zuki Abu Bakar Zakaria
The healing of load-bearing segmental defects in long bones is a challenge due to the complex nature of the weight that affects the bone part and due to bending, shearing, axial, and torsional forces. An innovative porous 3D scaffolds implant of CaCO3 aragonite nanocomposite derived from cockle shell was advanced for substitute bone solely for load-bearing cases. The biomechanical characteristics of such materials were designed to withstand cortical bone strength. In promoting bone growth to the implant material, an ideal surface permeability was formed by means of freeze drying and by adding copolymers to the materials...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29213311/expression-of-adha-from-different-organisms-in-clostridium-thermocellum
#6
Tianyong Zheng, Jingxuan Cui, Hye Ri Bae, Lee R Lynd, Daniel G Olson
Background: Clostridium thermocellum is a cellulolytic anaerobic thermophile that is a promising candidate for consolidated bioprocessing of lignocellulosic biomass into biofuels such as ethanol. It was previously shown that expressing Thermoanaerobacterium saccharolyticum adhA in C. thermocellum increases ethanol yield.In this study, we investigated expression of adhA genes from different organisms in Clostridium thermocellum . Methods: Based on sequence identity to T...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29137454/determination-of-cancer-cell-based-ph-sensitive-fluorescent-carbon-nanoparticles-of-cross-linked-polydopamine-by-fluorescence-sensing-of-alkaline-phosphatase-activity-on-coated-surfaces-and-aqueous-solution
#7
Eun Bi Kang, Cheong A Choi, Zihnil Adha Islamy Mazrad, Sung Han Kim, Insik In, Sung Young Park
The tumor-specific sensitive fluorescence sensing of cellular alkaline phosphatase (ALP) activity on the basis of host-guest specific and pH sensitivity was conducted on coated surfaces and aqueous states. Cross-linked fluorescent nanoparticles (C-FNP) consisting of β-cyclodextrin (β-CD)/boronic acid (BA) and fluorescent hyaluronic acid [FNP(HA)] were conjugated to fluorescent polydopamine [FNP(pDA)]. To determine the quenching effect of this system, hydrolysis of 4-nitrophenyl phosphate (NPP) to 4-nitrophenol (NP) was performed in the cavity of β-CD in the presence of ALP activated photoinduced electron transfer (PET) between NP and C-FNP...
December 19, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29118258/adha-annual-conference-research-posters
#8
(no author information available yet)
No abstract text is available yet for this article.
April 2017: Journal of Dental Hygiene: JDH
https://www.readbyqxmd.com/read/29118152/dentsply-sirona-adha-graduate-student-clinician-research-abstracts
#9
(no author information available yet)
No abstract text is available yet for this article.
February 2017: Journal of Dental Hygiene: JDH
https://www.readbyqxmd.com/read/29062482/ocular-tuberculosis-in-hospital-universiti-sains-malaysia-a-case-series
#10
M Shahidatul-Adha, E Zunaina, A T Liza-Sharmini, W H Wan-Hazabbah, I Shatriah, I Mohtar, Y Azhany, H Adil
INTRODUCTION: Ocular tuberculosis (TB) encompasses a broad spectrum of clinical manifestations affecting different structures of the eye. It is caused by Mycobacterium tuberculosis , a great ancient organism that induces various types of diseases and unfavorable outcomes if unrecognized and not well treated. PURPOSE: To report the clinical profile of 34 ocular TB cases observed during 6 years period in Hospital Universiti Sains Malaysia (HUSM). METHOD: A retrospective review of medical records from 34 patients diagnosed with ocular TB in HUSM from January 2011 until December 2016...
December 2017: Annals of Medicine and Surgery
https://www.readbyqxmd.com/read/28978312/enhanced-ethanol-formation-by-clostridium-thermocellum-via-pyruvate-decarboxylase
#11
Liang Tian, Skyler J Perot, Shuen Hon, Jilai Zhou, Xiaoyu Liang, Jason T Bouvier, Adam M Guss, Daniel G Olson, Lee R Lynd
BACKGROUND: Pyruvate decarboxylase (PDC) is a well-known pathway for ethanol production, but has not been demonstrated for high titer ethanol production at temperatures above 50 °C. RESULT: Here we examined the thermostability of eight PDCs. The purified bacterial enzymes retained 20% of activity after incubation for 30 min at 55 °C. Expression of these PDC genes, except the one from Zymomonas mobilis, improved ethanol production by Clostridium thermocellum...
October 4, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28966186/relationship-of-dental-hygiene-students-engagement-practices-to-their-academic-achievement
#12
Susan M Leiken
Students in many fields have been found to learn more the more they are engaged in both the academic and social aspects of the learning experience. The aims of this study were to determine the level of dental hygiene students' engagement in their educational programs and in student chapters of the American Dental Hygienists' Association (ADHA) and to assess the relationship of that engagement to students' success as measured by cumulative GPA. All 12,000 dental hygiene students (6,000 in the first year and 6,000 in the second year of two-year programs) enrolled in the 334 accredited U...
October 2017: Journal of Dental Education
https://www.readbyqxmd.com/read/28932219/improving-acetic-acid-production-by-over-expressing-pqq-adh-in-acetobacter-pasteurianus
#13
Xuefeng Wu, Hongli Yao, Lili Cao, Zhi Zheng, Xiaoju Chen, Min Zhang, Zhaojun Wei, Jieshun Cheng, Shaotong Jiang, Lijun Pan, Xingjiang Li
Pyrroquinoline quinone-dependent alcohol dehydrogenase (PQQ-ADH) is a key enzyme in the ethanol oxidase respiratory chain of acetic acid bacteria (AAB). To investigate the effect of PQQ-ADH on acetic acid production by Acetobacter pasteurianus JST-S, subunits I (adhA) and II (adhB) of PQQ-ADH were over-expressed, the fermentation parameters and the metabolic flux analysis were compared in the engineered strain and the original one. The acetic acid production was improved by the engineered strain (61.42 g L(-1)) while the residual ethanol content (4...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28876888/design-of-surface-coatable-nir-responsive-fluorescent-nanoparticles-with-pei-passivation-for-bacterial-detection-and-killing
#14
Zihnil Adha Islamy Mazrad, Cheong A Choi, Yong Min Kwon, Insik In, Kang Dae Lee, Sung Young Park
The ability to quickly detect and kill bacteria is crucial in the realm of antibiotic resistance. In this study, we synthesized a detection probe consisting of polyethylenimine (PEI)-passivated polydopamine-based fluorescent carbon (FDA:PEI) nanoparticles, generating a cationic adhesive material for bacterial detection that is surface-coatable, photothermal, and antibacterial. The cationic FDA:PEI nanoparticles effectively bound to the anionic bacterial cell wall, resulting in a dramatic quenching effect visible in fluorescence spectra and confocal images...
September 27, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28799892/the-impact-of-socio-cultural-factors-on-transmission-of-taenia-spp-and-echinococcus-granulosus-in-kosovo
#15
M Alishani, K Sherifi, A Rexhepi, A Hamidi, M T Armua-Fernandez, F Grimm, D Hegglin, P Deplazes
Echinococcus granulosus sensu latu (s.l.) and Taenia hydatigena are common parasites of ruminant intermediate hosts in the Balkans. Transmission is linked mainly to home slaughtering and the feeding of infected organs to dogs. In Kosovo, many old sheep are slaughtered particularly during Eid al-Adha (Feast of Sacrifice). To determine whether this tradition could affect parasite transmission, we compared the probability of 504 dogs to contract taenid infections after deworming during one period before Eid al-Adha and a similar period beginning with this event...
November 2017: Parasitology
https://www.readbyqxmd.com/read/28731532/formate-and-nitrate-utilization-in-enterobacter-aerogenes-for-semi-anaerobic-production-of-isobutanol
#16
Hwi-Min Jung, Yong Hwan Kim, Min-Kyu Oh
Anaerobic bioprocessing is preferred because of its economic advantages. However, low productivity and decreased growth of the host strain have limited the use of the anaerobic process. Anaerobic respiration can be applied to anoxic processing using formate and nitrate metabolism to improve the productivity of value-added metabolites. A isobutanol-producing strains is constructed using Enterobacter aerogenes as a host strain by metabolic engineering approaches. The byproduct pathway (ldhA, budA, and pflB) is knocked out, and heterologous keto-acid decarboxylase (kivD) and alcohol dehydrogenase (adhA) are expressed along with the L-valine synthesis pathway (ilvCD and budB)...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28719145/preparation-of-carbon-dot-based-ratiometric-fluorescent-probes-for-cellular-imaging-from-curcuma-longa
#17
Zihnil Adha Islamy Mazrad, Eun Bi Kang, Insik In, Sung Young Park
This work derived biocompatible and stable probes based on fluorescent nanoparticles (FNPs) from a natural source, Curcuma longa. The multi-color fluorescence emissions from carbonized Curcuma longa (C-FNPs) obtained through defined dehydration conditions are soluble in water and have a small particle size (~17 nm). The surface passivation with polyethylene glycol (PEG) capped with amine groups in FNPs (P-FNPs) generated a probe with a higher quantum yield and longer fluorescence lifetime than obtained with C-FNPs...
February 2018: Luminescence: the Journal of Biological and Chemical Luminescence
https://www.readbyqxmd.com/read/28663138/the-ethanol-pathway-from-thermoanaerobacterium-saccharolyticum-improves-ethanol-production-in-clostridium-thermocellum
#18
Shuen Hon, Daniel G Olson, Evert K Holwerda, Anthony A Lanahan, Sean J L Murphy, Marybeth I Maloney, Tianyong Zheng, Beth Papanek, Adam M Guss, Lee R Lynd
Clostridium thermocellum ferments cellulose, is a promising candidate for ethanol production from cellulosic biomass, and has been the focus of studies aimed at improving ethanol yield. Thermoanaerobacterium saccharolyticum ferments hemicellulose, but not cellulose, and has been engineered to produce ethanol at high yield and titer. Recent research has led to the identification of four genes in T. saccharolyticum involved in ethanol production: adhE, nfnA, nfnB and adhA. We introduced these genes into C. thermocellum and observed significant improvements to ethanol yield, titer, and productivity...
July 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28455635/seasonality-in-hospital-admissions-of-crimean-congo-hemorrhagic-fever-and-its-dependence-on-ambient-temperature-empirical-evidence-from-pakistan
#19
Tariq Abbas, Zhiwei Xu, Muhammad Younus, Abdul Qayyum, Muhammad T Riaz
Crimean-Congo hemorrhagic fever (CCHF) has been reported from all provinces of Pakistan. Little is known about the seasonal variations in the disease and its association with weather conditions. In this study, we explored time-series data about monthly number of CCHF admissions (2007-2010) in three public sector hospitals of Quetta-the capital city of Baluchistan province of Pakistan. Cosinor analysis was carried out to investigate seasonality in the data. To assess the effect of average monthly ambient temperature (°C) on disease, a distributed lag nonlinear model (DLNM) was applied...
November 2017: International Journal of Biometeorology
https://www.readbyqxmd.com/read/28338219/human-orf-complicated-by-epidermolysis-bullosa-acquisita
#20
E Zuelgaray, C Salle de Chou, J Gottlieb, M Battistella, M D Vignon-Pennamen, M Bagot, F Guibal, J D Bouaziz
Orf is a DNA parapoxvirus transmitted to humans by contact with infected goats and sheep. Many complications have been reported after orf infection, including erythema multiforme. A few cases of autoimmune bullous dermatosis complicating orf disease have been reported to date. They are usually characterized by tense blister eruptions with or without mucosal involvement; linear deposition of C3, IgG and/or IgA along the basement membrane; and negativity of indirect immunofluorescence analysis and enzyme-linked immunosorbent assay (ELISA) (performed in four of 11 reported cases)...
March 24, 2017: British Journal of Dermatology
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