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https://www.readbyqxmd.com/read/28978312/enhanced-ethanol-formation-by-clostridium-thermocellum-via-pyruvate-decarboxylase
#1
Liang Tian, Skyler J Perot, Shuen Hon, Jilai Zhou, Xiaoyu Liang, Jason T Bouvier, Adam M Guss, Daniel G Olson, Lee R Lynd
BACKGROUND: Pyruvate decarboxylase (PDC) is a well-known pathway for ethanol production, but has not been demonstrated for high titer ethanol production at temperatures above 50 °C. RESULT: Here we examined the thermostability of eight PDCs. The purified bacterial enzymes retained 20% of activity after incubation for 30 min at 55 °C. Expression of these PDC genes, except the one from Zymomonas mobilis, improved ethanol production by Clostridium thermocellum...
October 4, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28966186/relationship-of-dental-hygiene-students-engagement-practices-to-their-academic-achievement
#2
Susan M Leiken
Students in many fields have been found to learn more the more they are engaged in both the academic and social aspects of the learning experience. The aims of this study were to determine the level of dental hygiene students' engagement in their educational programs and in student chapters of the American Dental Hygienists' Association (ADHA) and to assess the relationship of that engagement to students' success as measured by cumulative GPA. All 12,000 dental hygiene students (6,000 in the first year and 6,000 in the second year of two-year programs) enrolled in the 334 accredited U...
October 2017: Journal of Dental Education
https://www.readbyqxmd.com/read/28932219/improving-acetic-acid-production-by-over-expressing-pqq-adh-in-acetobacter-pasteurianus
#3
Xuefeng Wu, Hongli Yao, Lili Cao, Zhi Zheng, Xiaoju Chen, Min Zhang, Zhaojun Wei, Jieshun Cheng, Shaotong Jiang, Lijun Pan, Xingjiang Li
Pyrroquinoline quinone-dependent alcohol dehydrogenase (PQQ-ADH) is a key enzyme in the ethanol oxidase respiratory chain of acetic acid bacteria (AAB). To investigate the effect of PQQ-ADH on acetic acid production by Acetobacter pasteurianus JST-S, subunits I (adhA) and II (adhB) of PQQ-ADH were over-expressed, the fermentation parameters and the metabolic flux analysis were compared in the engineered strain and the original one. The acetic acid production was improved by the engineered strain (61.42 g L(-1)) while the residual ethanol content (4...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28876888/design-of-surface-coatable-nir-responsive-fluorescent-nanoparticles-with-pei-passivation-for-bacterial-detection-and-killing
#4
Zihnil Adha Islamy Mazrad, Cheong A Choi, Yong Min Kwon, Insik In, Kang Dae Lee, Sung Young Park
The ability to quickly detect and kill bacteria is crucial in the realm of antibiotic resistance. In this study, we synthesized a detection probe consisting of polyethylenimine (PEI)-passivated polydopamine-based fluorescent carbon (FDA:PEI) nanoparticles, generating a cationic adhesive material for bacterial detection that is surface-coatable, photothermal, and antibacterial. The cationic FDA:PEI nanoparticles effectively bound to the anionic bacterial cell wall, resulting in a dramatic quenching effect visible in fluorescence spectra and confocal images...
September 27, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28799892/the-impact-of-socio-cultural-factors-on-transmission-of-taenia-spp-and-echinococcus-granulosus-in-kosovo
#5
M Alishani, K Sherifi, A Rexhepi, A Hamidi, M T Armua-Fernandez, F Grimm, D Hegglin, P Deplazes
Echinococcus granulosus sensu latu (s.l.) and Taenia hydatigena are common parasites of ruminant intermediate hosts in the Balkans. Transmission is linked mainly to home slaughtering and the feeding of infected organs to dogs. In Kosovo, many old sheep are slaughtered particularly during Eid al-Adha (Feast of Sacrifice). To determine whether this tradition could affect parasite transmission, we compared the probability of 504 dogs to contract taenid infections after deworming during one period before Eid al-Adha and a similar period beginning with this event...
November 2017: Parasitology
https://www.readbyqxmd.com/read/28731532/formate-and-nitrate-utilization-in-enterobacter-aerogenes-for-semi-anaerobic-production-of-isobutanol
#6
Hwi-Min Jung, Yong Hwan Kim, Min-Kyu Oh
Anaerobic bioprocessing is preferred because of its economic advantages. However, low productivity and decreased growth of the host strain have limited the use of the anaerobic process. Anaerobic respiration can be applied to anoxic processing using formate and nitrate metabolism to improve the productivity of value-added metabolites. We constructed isobutanol-producing strains using Enterobacter aerogenes as a host strain by metabolic engineering approaches. The byproduct pathway (ldhA, budA, and pflB) was knocked out, and heterologous keto-acid decarboxylase (kivD) and alcohol dehydrogenase (adhA) were expressed along with the L-valine synthesis pathway (ilvCD and budB)...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28719145/preparation-of-carbon-dot-based-ratiometric-fluorescent-probes-for-cellular-imaging-from-curcuma-longa
#7
Zihnil Adha Islamy Mazrad, Eun Bi Kang, Insik In, Sung Young Park
This work derived biocompatible and stable probes based on fluorescent nanoparticles (FNPs) from a natural source, Curcuma longa. The multi-color fluorescence emissions from carbonized Curcuma longa (C-FNPs) obtained through defined dehydration conditions are soluble in water and have a small particle size (~17 nm). The surface passivation with polyethylene glycol (PEG) capped with amine groups in FNPs (P-FNPs) generated a probe with a higher quantum yield and longer fluorescence lifetime than obtained with C-FNPs...
July 18, 2017: Luminescence: the Journal of Biological and Chemical Luminescence
https://www.readbyqxmd.com/read/28663138/the-ethanol-pathway-from-thermoanaerobacterium-saccharolyticum-improves-ethanol-production-in-clostridium-thermocellum
#8
Shuen Hon, Daniel G Olson, Evert K Holwerda, Anthony A Lanahan, Sean J L Murphy, Marybeth I Maloney, Tianyong Zheng, Beth Papanek, Adam M Guss, Lee R Lynd
Clostridium thermocellum ferments cellulose, is a promising candidate for ethanol production from cellulosic biomass, and has been the focus of studies aimed at improving ethanol yield. Thermoanaerobacterium saccharolyticum ferments hemicellulose, but not cellulose, and has been engineered to produce ethanol at high yield and titer. Recent research has led to the identification of four genes in T. saccharolyticum involved in ethanol production: adhE, nfnA, nfnB and adhA. We introduced these genes into C. thermocellum and observed significant improvements to ethanol yield, titer, and productivity...
July 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28455635/seasonality-in-hospital-admissions-of-crimean-congo-hemorrhagic-fever-and-its-dependence-on-ambient-temperature-empirical-evidence-from-pakistan
#9
Tariq Abbas, Zhiwei Xu, Muhammad Younus, Abdul Qayyum, Muhammad T Riaz
Crimean-Congo hemorrhagic fever (CCHF) has been reported from all provinces of Pakistan. Little is known about the seasonal variations in the disease and its association with weather conditions. In this study, we explored time-series data about monthly number of CCHF admissions (2007-2010) in three public sector hospitals of Quetta-the capital city of Baluchistan province of Pakistan. Cosinor analysis was carried out to investigate seasonality in the data. To assess the effect of average monthly ambient temperature (°C) on disease, a distributed lag nonlinear model (DLNM) was applied...
April 28, 2017: International Journal of Biometeorology
https://www.readbyqxmd.com/read/28338219/human-orf-complicated-by-epidermolysis-bullosa-acquisita
#10
E Zuelgaray, C Salle de Chou, J Gottlieb, M Battistella, M D Vignon-Pennamen, M Bagot, F Guibal, J D Bouaziz
Orf is a DNA parapoxvirus transmitted to humans by contact with infected goats and sheep. Many complications have been reported after Orf infection including erythema multiforme. A few cases of auto-immune bullous dermatosis complicating Orf disease have been reported to date, usually characterized by tense blisters eruptions with or without mucosal involvement, linear deposition of C3, IgG and/or IgA along the basement membrane and negativity of indirect immunofluorescence analysis and ELISA assays (performed in 4 of 11 reported cases) against target antigens of bullous pemphigoid, mucous membrane pemphigoid or epidermolysis bullosa acquisita, except one case of mucosal pemphigoid with antilaminin-332 antibodies...
March 24, 2017: British Journal of Dermatology
https://www.readbyqxmd.com/read/28294094/market-organization-and-animal-genetic-resource-management-a-revealed-preference-analysis-of-sheep-pricing
#11
K Tindano, N Moula, P Leroy, A Traoré, N Antoine-Moussiaux
Farm animal genetic resources are threatened worldwide. Participation in markets, while representing a crucial way out of poverty for many smallholders, affects genetic management choices with associated sustainability concerns. This paper proposes a contextualized study of the interactions between markets and animal genetic resources management, in the case of sheep markets in Ouagadougou, Burkina Faso. It focusses on the organization of marketing chains and the valuation of genetic characteristics by value chain actors...
March 15, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28194879/ethanol-production-by-the-hyperthermophilic-archaeon-pyrococcus-furiosus-by-expression-of-bacterial-bifunctional-alcohol-dehydrogenases
#12
Matthew W Keller, Gina L Lipscomb, Diep M Nguyen, Alexander T Crowley, Gerrit J Schut, Israel Scott, Robert M Kelly, Michael W W Adams
Ethanol is an important target for the renewable production of liquid transportation fuels. It can be produced biologically from pyruvate, via pyruvate decarboxylase, or from acetyl-CoA, by alcohol dehydrogenase E (AdhE). Thermophilic bacteria utilize AdhE, which is a bifunctional enzyme that contains both acetaldehyde dehydrogenase and alcohol dehydrogenase activities. Many of these organisms also contain a separate alcohol dehydrogenase (AdhA) that generates ethanol from acetaldehyde, although the role of AdhA in ethanol production is typically not clear...
February 14, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28160048/alcohol-dehydrogenase-adha-plays-a-role-in-ethanol-tolerance-in-model-cyanobacterium-synechocystis-sp-pcc-6803
#13
Rebeca Vidal
The protein AdhA from the cyanobacterium Synechocystis sp. PCC 6803 (hereafter Synechocystis) has been previously reported to show alcohol dehydrogenase activity towards ethanol and both NAD and NADP. This protein is currently being used in genetically modified strains of Synechocystis capable of synthesizing ethanol showing the highest ethanol productivities. In the present work, mutant strains of Synechocystis lacking AdhA have been constructed and tested for tolerance to ethanol. The lack of AdhA in the wild-type strain reduces survival to externally added ethanol at lethal concentration of 4% (v/v)...
April 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28100303/ethanol-glycogen-and-glucosylglycerol-represent-competing-carbon-pools-in-ethanol-producing-cells-of-synechocystis-sp-pcc-6803-under-high-salt-conditions
#14
Nadin Pade, Stefan Mikkat, Martin Hagemann
Cyanobacteria are photoautotrophic micro-organisms, which are increasingly being used as microbial cell factories to produce, for example, ethanol directly from solar energy and CO2. Here, we analysed the effects of different salt concentrations on an ethanol-producing strain of Synechocystis sp. PCC 6803 that overexpresses the pyruvate decarboxylase (pdc) from Zymomonas mobilis and the native alcohol dehydrogenase (adhA). Moderate salinities of 2 % NaCl had no negative impact on ethanol production, whereas the addition of 4 % NaCl resulted in significantly decreased ethanol yields compared to low-salt conditions...
March 2017: Microbiology
https://www.readbyqxmd.com/read/28078513/determining-the-roles-of-the-three-alcohol-dehydrogenases-adha-adhb-and-adhe-in-thermoanaerobacter-ethanolicus-during-ethanol-formation
#15
Jilai Zhou, Xiongjun Shao, Daniel G Olson, Sean Jean-Loup Murphy, Liang Tian, Lee R Lynd
Thermoanaerobacter ethanolicus is a promising candidate for biofuel production due to the broad range of substrates it can utilize and its high ethanol yield compared to other thermophilic bacteria, such as Clostridium thermocellum. Three alcohol dehydrogenases, AdhA, AdhB and AdhE, play key roles in ethanol formation. To study their physiological roles during ethanol formation, we deleted them separately and in combination. Previously, it has been thought that both AdhB and AdhE were bifunctional alcohol dehydrogenases...
May 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27987660/preparation-of-dual-responsive-hybrid-fluorescent-nano-probe-based-on-graphene-oxide-and-boronic-acid-bodipy-conjugated-polymer-for-cell-imaging
#16
Khoerunnisa, Eun Bi Kang, Zihnil Adha Islamy Mazrad, Gibaek Lee, Insik In, Sung Young Park
Here, we report a pH- and thermo-responsive fluorescent nanomaterial of functionalized reduced graphene oxide (rGO) with cross-linked polymer produced via catechol-boronate diol binding mechanism. When conjugated with the hydrophobic dye boron dipyrromethane (BODIPY), this material can act as a dual-responsive nanoplatform for cells imaging. 2-Chloro-3',4'-dihydroxyacetophenone (CCDP)-quaternized-poly(dimethylaminoethyl methacrylate-co-N-isopropylacrylamide) [C-PDN] was cross-linked with BODIPY and 4-chlorophenyl boronic acid (BA)-quaternized-poly(ethylene glycol)-g-poly(dimethylaminoethyl methacrylate-co-N-isopropylacrylamide) [BB-PPDN]...
February 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27849176/both-adhe-and-a-separate-nadph-dependent-alcohol-dehydrogenase-adha-are-necessary-for-high-ethanol-production-in-thermoanaerobacterium-saccharolyticum
#17
Tianyong Zheng, Daniel G Olson, Sean J Murphy, Xiongjun Shao, Liang Tian, Lee R Lynd
Thermoanaerobacterium saccharolyticum has been engineered to produce ethanol at ∼90% theoretical yield and titer of 70 g/L. Its ethanol-producing ability has drawn attention to its metabolic pathways, which could potentially be transferred to other organisms of interest. Here we report that the iron-containing AdhA is important for ethanol production in the high-ethanol strain of T. saccharolyticum (LL1049). A single-gene deletion of adhA in LL1049 reduced ethanol production by ∼50%, whereas multiple gene deletions of all annotated alcohol dehydrogenases except adhA and adhE did not affect ethanol production...
November 14, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27825519/rapid-fluorometric-bacteria-detection-assay-and-photothermal-effect-by-fluorescent-polymer-of-coated-surfaces-and-aqueous-state
#18
Zihnil Adha Islamy Mazrad, Insik In, Kang-Dae Lee, Sung Young Park
A fluorescent dye and a photothermal agent were grafted onto a cationic polymer for rapid and simple bacteria detection in liquid and solid phase based fluorescence on/off. The integrated poly(vinylpyrrolidone) (PVP) backbone with catechol and bromoethane moieties possesses unique optical properties due to the presence of boron dipyrromethane (BODIPY) and near infared NIR-responsive IR825 (F-PVP). The cationic segments showed distinct fluorescence quenching patterns after interaction with gram-positive and gram-negative bacteria via polyion complex interactions...
March 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27570812/dataset-of-traumatic-myiasis-observed-for-three-dominant-screw-worm-species-in-north-west-pakistan-with-first-report-of-wohlfahrtia-magnifica-schiner
#19
Farrah Zaidi, Syeda Hira Fatima, Ayesha Gul
Regional surveys were carried out in different parts of North West Pakistan among domestic animals (N=57,921) including pets and livestock identifying cases of traumatic myiasis (n=1037). A total of four surveys focused general livestock population during Eid ul Adha (Eid surveys; incidence=1.21%) while another four surveys (Miscellaneous surveys; incidence=7.34%) targeted animal population brought to veterinary hospitals and dispensaries. Timeframe spanned four years from 2012 to 2015. Maggots were sampled and location of the wound was recorded for each host...
September 2016: Data in Brief
https://www.readbyqxmd.com/read/27473771/factors-driving-changes-in-freshwater-mussel-bivalvia-unionida-diversity-and-distribution-in-peninsular-malaysia
#20
Alexandra Zieritz, Manuel Lopes-Lima, Arthur E Bogan, Ronaldo Sousa, Samuel Walton, Khairul Adha A Rahim, John-James Wilson, Pei-Yin Ng, Elsa Froufe, Suzanne McGowan
Freshwater mussels (Bivalvia, Unionida) fulfil important ecosystem functions and are one of the most threatened freshwater taxa globally. Knowledge of freshwater mussel diversity, distribution and ecology in Peninsular Malaysia is extremely poor, and the conservation status of half of the species presumed to occur in the region has yet to be assessed. We conducted the first comprehensive assessment of Peninsular Malaysia's freshwater mussels based on species presence/absence and environmental data collected from 155 sites spanning all major river catchments and diverse habitat types...
November 15, 2016: Science of the Total Environment
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