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Jilai Zhou, Xiongjun Shao, Daniel G Olson, Sean Jean-Loup Murphy, Liang Tian, Lee R Lynd
Thermoanaerobacter ethanolicus is a promising candidate for biofuel production due to the broad range of substrates it can utilize and its high ethanol yield compared to other thermophilic bacteria, such as Clostridium thermocellum. Three alcohol dehydrogenases, AdhA, AdhB and AdhE, play key roles in ethanol formation. To study their physiological roles during ethanol formation, we deleted them separately and in combination. Previously, it has been thought that both AdhB and AdhE were bifunctional alcohol dehydrogenases...
January 11, 2017: Journal of Industrial Microbiology & Biotechnology
Khoerunnisa, Eun Bi Kang, Zihnil Adha Islamy Mazrad, Gibaek Lee, Insik In, Sung Young Park
Here, we report a pH- and thermo-responsive fluorescent nanomaterial of functionalized reduced graphene oxide (rGO) with cross-linked polymer produced via catechol-boronate diol binding mechanism. When conjugated with the hydrophobic dye boron dipyrromethane (BODIPY), this material can act as a dual-responsive nanoplatform for cells imaging. 2-Chloro-3',4'-dihydroxyacetophenone (CCDP)-quaternized-poly(dimethylaminoethyl methacrylate-co-N-isopropylacrylamide) [C-PDN] was cross-linked with BODIPY and 4-chlorophenyl boronic acid (BA)-quaternized-poly(ethylene glycol)-g-poly(dimethylaminoethyl methacrylate-co-N-isopropylacrylamide) [BB-PPDN]...
February 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Tianyong Zheng, Daniel G Olson, Sean J Murphy, Xiongjun Shao, Liang Tian, Lee R Lynd
: Thermoanaerobacterium saccharolyticum has been engineered to produce ethanol at ∼90% theoretical yield and titer of 70 g/L. Its ethanol-producing ability has drawn attention to its metabolic pathways, which could potentially be transferred to other organisms of interest. Here we report that the iron-containing AdhA is important for ethanol production in the high-ethanol strain of T. saccharolyticum (LL1049). A single-gene deletion of adhA in LL1049 reduced ethanol production by ∼50%, whereas multiple gene deletions of all annotated alcohol dehydrogenases except adhA and adhE did not affect ethanol production...
November 14, 2016: Journal of Bacteriology
Zihnil Adha Islamy Mazrad, Insik In, Kang-Dae Lee, Sung Young Park
A fluorescent dye and a photothermal agent were grafted onto a cationic polymer for rapid and simple bacteria detection in liquid and solid phase based fluorescence on/off. The integrated poly(vinylpyrrolidone) (PVP) backbone with catechol and bromoethane moieties possesses unique optical properties due to the presence of boron dipyrromethane (BODIPY) and near infared NIR-responsive IR825 (F-PVP). The cationic segments showed distinct fluorescence quenching patterns after interaction with gram-positive and gram-negative bacteria via polyion complex interactions...
March 15, 2017: Biosensors & Bioelectronics
Farrah Zaidi, Syeda Hira Fatima, Ayesha Gul
Regional surveys were carried out in different parts of North West Pakistan among domestic animals (N=57,921) including pets and livestock identifying cases of traumatic myiasis (n=1037). A total of four surveys focused general livestock population during Eid ul Adha (Eid surveys; incidence=1.21%) while another four surveys (Miscellaneous surveys; incidence=7.34%) targeted animal population brought to veterinary hospitals and dispensaries. Timeframe spanned four years from 2012 to 2015. Maggots were sampled and location of the wound was recorded for each host...
September 2016: Data in Brief
Alexandra Zieritz, Manuel Lopes-Lima, Arthur E Bogan, Ronaldo Sousa, Samuel Walton, Khairul Adha A Rahim, John-James Wilson, Pei-Yin Ng, Elsa Froufe, Suzanne McGowan
Freshwater mussels (Bivalvia, Unionida) fulfil important ecosystem functions and are one of the most threatened freshwater taxa globally. Knowledge of freshwater mussel diversity, distribution and ecology in Peninsular Malaysia is extremely poor, and the conservation status of half of the species presumed to occur in the region has yet to be assessed. We conducted the first comprehensive assessment of Peninsular Malaysia's freshwater mussels based on species presence/absence and environmental data collected from 155 sites spanning all major river catchments and diverse habitat types...
November 15, 2016: Science of the Total Environment
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No abstract text is available yet for this article.
June 2016: Journal of Dental Hygiene: JDH
Indro Neil Ghosh, Robert Landick
The optimization of synthetic pathways is a central challenge in metabolic engineering. OptSSeq (Optimization by Selection and Sequencing) is one approach to this challenge. OptSSeq couples selection of optimal enzyme expression levels linked to cell growth rate with high-throughput sequencing to track enrichment of gene expression elements (promoters and ribosome-binding sites) from a combinatorial library. OptSSeq yields information on both optimal and suboptimal enzyme levels, and helps identify constraints that limit maximal product formation...
December 16, 2016: ACS Synthetic Biology
Ann Battrell, Ann Lynch, Pam Steinbach
UNLABELLED: With the dental hygiene profession undergoing tremendous transformation as greater and more diverse workplace opportunities present themselves, the American Dental Hygienists' Association (ADHA) is leading the effort to ensure that dental hygienists are prepared to take advantage of these growing opportunities in today's constantly evolving health care landscape. BACKGROUND AND PURPOSE: ADHA's vision statement calls for the integration of dental hygienists into the health care delivery system as essential primary care providers to expand access to oral health care...
June 2016: Journal of Evidence-based Dental Practice
Mustafa Basturk, Yavuz Katirci, Tarik Ocak, Mehmet S Yurdakul, Arif Duran, Isa Baspinar
BACKGROUND: During the Eid al-Adha ("Sacrifice Feast") religious holiday in Muslim communities animal sacrifices are made over a period of 3 days every year. OBJECTIVES: The aim of this study was to determine the type of sacrifice-related injuries, the characteristics of patients, treatments for injuries, and relationships between these factors to determine precautions that could be taken to avoid or mitigate sacrifice-related injuries. DESIGN: Retrospective study of medical records...
March 2016: Annals of Saudi Medicine
Huan Zhang, Lulu Shi, Jinping Lin, Ming Sun, Dongzhi Wei
OBJECTIVES: To investigate the roles of adhS, which encodes the AdhS subunit of membrane-bound alcohol dehydrogenase (mADH) in Gluconobacter oxydans DSM2003, and to rationally improve mADH activity. RESULTS: adhS was identified and overexpressed in G. oxydans DSM2003. Its overexpression promoted the AdhA subunit which serves as the primary dehydrogenase transfer from the periplasmic space to the periplasmic surface of the membrane thereby increasing the amount of active mADH and thus enhancing mADH activity up to 1...
July 2016: Biotechnology Letters
Shiyuan Xiao, Jingliang Xu, Xiaoyan Chen, Xiekun Li, Yu Zhang, Zhenhong Yuan
Biofuel offers a promising solution to the adverse environmental problems and depletion in reserves of fossil fuels. Higher alcohols including 3-methyl-1-butanol were paid much more attention as fuel substitute in recent years, due to its similar properties to gasoline. In the present work, 3-methyl-1-butanol production in engineered Corynebacterium glutamicum was studied. α-Ketoisovalerate decarboxylase gene (kivd) from Lactococcus lactis combined with alcohol dehydrogenase gene (adh2, adhA, and adh3) from three organisms were overexpressed in C...
May 2016: Molecular Biotechnology
Avinash S Bidra, Diane M Daubert, Lily T Garcia, Timothy F Kosinski, Conrad A Nenn, John A Olsen, Jeffrey A Platt, Susan S Wingrove, Nancy Deal Chandler, Donald A Curtis
PURPOSE: To provide guidelines for patient recall regimen, professional maintenance regimen, and at-home maintenance regimen for patients with tooth-borne and implant-borne removable and fixed restorations. MATERIALS AND METHODS: The American College of Prosthodontists (ACP) convened a scientific panel of experts appointed by the ACP, American Dental Association (ADA), Academy of General Dentistry (AGD), and American Dental Hygienists Association (ADHA) who critically evaluated and debated recently published findings from two systematic reviews on this topic...
January 2016: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
Lauren Smith, Margaret Walsh
PURPOSE: To assess California dental hygiene educators' perceptions of an application of the American Dental Hygienists' Association's (ADHA) advanced dental hygiene practitioner model (ADHP) in medical settings where the advanced dental hygiene practitioner collaborates in medical settings with other health professionals to meet clients' oral health needs. METHODS: In 2014, 30 directors of California dental hygiene programs were contacted to participate in and distribute an online survey to their faculty...
December 2015: Journal of Dental Hygiene: JDH
Christine Rogers, Tara B Johnson, JoAnn R Gurenlian
PURPOSE: Dual educational pathways exist for entry into the dental hygiene profession, namely associate and baccalaureate degrees. The purpose of this study was to examine practicing dental hygienists' perceptions, regarding the requirement of a baccalaureate degree as entry-to-practice for the profession. METHODS: A purposive sample of 800 dental hygienists licensed within New York State, both members and non-members of the Dental Hygienists' Association of the state of New York, were contacted via email and asked to participate in this Web-based survey...
June 2015: Journal of Dental Hygiene: JDH
Hakan Leblebicioglu, Mustafa Sunbul, Ziad A Memish, Jaffar A Al-Tawfiq, Hurrem Bodur, Aykut Ozkul, Ali Gucukoglu, Sadegh Chinikar, Zahra Hasan
Crimean-Congo hemorrhagic fever (CCHF) is endemic in Eurasian countries such as, Turkey, Pakistan, Afghanistan and Iran. CCHF virus is spread by the Hyalomma tick, which is found mainly on cattle and sheep. Muslim countries, in which these animals are sacrificed during Eid-Al-Adha, are among the countries where CCHF is endemic, and it has been observed that CCHF is associated with practices surrounding the Eid-ad-Adha festival. The dates for Eid-Al-Adha drift 10 days earlier in each year according to Georgian calendar...
September 2015: International Journal of Infectious Diseases: IJID
Fatemeh Hosseini, Nikmatul Adha, Rosilawati Zainol, Marzuki Isahak, Nahid Nemati
The main objective was to find association between basal cortisol and neighborhood-level stress. Systematic searches, including electronic and hand searches, were conducted. The most recent date of the search was July 26, 2013. Primary observational studies included if they considered stress related outcomes in the neighborhood context. Using the EndNote X7 advanced search option; the authors examined the abstracts and titles of the 18,092 articles to exclude obviously irrelevant studies, gray literature, discussion papers, reviews and, studies with no complete data...
October 2014: Iranian Journal of Public Health
Estefanos Tadesse, Tegene Negesse, Girma Abebe
A survey was conducted in Awassazuria district of southern Ethiopia to characterize sheep production system. A structured questionnaire was used to collect data. Using purposive sampling, a total of 120 households from the district were included in the survey. Collected data were analyzed using descriptive statistics. Result indicated that Kajima neighbourhood has more (p<0.05) grazing land than the others. Communal grazing, roadside grazing, enset (false banana, Ensete ventricosum), banana leaf and private grazing land were major feed resources for sheep...
October 2015: Tropical Animal Health and Production
Rebeca Vidal
Two-component systems have been extensively described in the control of gene expression in response to different environmental signals in the cyanobacterium Synechocystis sp. PCC 6803. The Hik34-Rre1 two-component system has been shown to regulate a set of genes under certain stress conditions. Some evidence indicates that another histidine kinase, probably Hik2, is acting upstream of Rre1 in the regulation of some genes in response to hyperosmotic and salt stress. In the present study, a mis-annotation of the Rre1 protein has been identified and the correct version has been functionally characterized in vitro...
April 2015: FEMS Microbiology Letters
Saúl Gómez-Manzo, José E Escamilla, Abigail González-Valdez, Gabriel López-Velázquez, América Vanoye-Carlo, Jaime Marcial-Quino, Ignacio de la Mora-de la Mora, Itzhel Garcia-Torres, Sergio Enríquez-Flores, Martha Lucinda Contreras-Zentella, Roberto Arreguín-Espinosa, Peter M H Kroneck, Martha Elena Sosa-Torres
Gluconacetobacter diazotrophicus is a N2-fixing bacterium endophyte from sugar cane. The oxidation of ethanol to acetic acid of this organism takes place in the periplasmic space, and this reaction is catalyzed by two membrane-bound enzymes complexes: the alcohol dehydrogenase (ADH) and the aldehyde dehydrogenase (ALDH). We present strong evidence showing that the well-known membrane-bound Alcohol dehydrogenase (ADHa) of Ga. diazotrophicus is indeed a double function enzyme, which is able to use primary alcohols (C2-C6) and its respective aldehydes as alternate substrates...
2015: International Journal of Molecular Sciences
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