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https://www.readbyqxmd.com/read/28720687/transcriptional-profiling-reveals-functional-dichotomy-between-human-slan-non-classical-monocytes-and-myeloid-dendritic-cells
#1
Nathalie van Leeuwen-Kerkhoff, Kristina Lundberg, Theresia M Westers, Shahram Kordasti, Hetty J Bontkes, Tanja D de Gruijl, Malin Lindstedt, Arjan A van de Loosdrecht
Human 6-sulfo LacNac-positive (slan(+)) cells have been subject to a paradigm debate. They have previously been classified as a distinct dendritic cell (DC) subset. However, evidence has emerged that they may be more related to monocytes than to DCs. To gain deeper insight into the functional specialization of slan(+) cells, we have compared them with both conventional myeloid DC subsets (CD1c(+) and CD141(+)) in human peripheral blood (PB). With the use of genome-wide transcriptional profiling, as well as functional tests, we clearly show that slan(+) cells form a distinct, non-DC-like population...
July 18, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28716658/stool-antigen-immunodetection-for-diagnosis-of-giardia-duodenalis-infection-in-human-subjects-with-hiv-and-cancer
#2
Maryam Nooshadokht, Behjat Kalantari-Khandani, Iraj Sharifi, Hossein Kamyabi, Namal P M Liyanage, Laurel A Lagenaur, Martin F Kagnoff, Steven M Singer, Zahra Babaei, Shahram Solaymani-Mohammadi
Human infection with the protozoan parasite Giardia duodenalis is one the most common parasitic diseases worldwide. Higher incidence rates of giardiasis have been reported from human subjects with multiple debilitating chronic conditions, including hypogammaglobulinemia and common variable immunodeficiency (CVID). In the current study, stool specimens were collected from 199 individuals diagnosed with HIV or cancer and immunocompetent subjects. The sensitivity of microscopy-based detection on fresh stool preparations, trichrome staining and stool antigen immunodetection for the diagnosis of G...
July 14, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28713614/condition-medium-of-cerebrospinal-fluid-and-retinoic-acid-induces-the-transdifferentiation-of-human-dental-pulp-stem-cells-into-neuroglia-and-neural-like-cells
#3
Sara Haratizadeh, Maryam Nazm Bojnordi, Shahram Darabi, Narges Karimi, Mehrdad Naghikhani, Hatef Ghasemi Hamidabadi, Morteza Seifi
Cerebrospinal fluid (CSF) contains several molecules which are essential for neurogenesis. Human dental pulp stem cells (hDPSCs) are putatively neural crest cell-derived that can differentiate into neurons and glial cells under appropriate neurotrophic factors. The aim of this study was to induce differentiation of hDPSCs into neuroglial phenotypes using retinoic acid (RA) and CSF. The hDPSCs from an impacted third molar were isolated by mechanical and digestion and cultured. The cells have treated by 10(-7) µM RA (RA group) for 8 days, 10% CSF (CSF group) for 8 days and RA with CSF for 8 days (RA/CSF group)...
June 2017: Anatomy & Cell Biology
https://www.readbyqxmd.com/read/28711170/early-selenium-treatment-for-traumatic-brain-injury-does-it-improve-survival-and-functional-outcome
#4
Hosseinali Khalili, Rebecka Ahl, Yang Cao, Shahram Paydar, Gabriel Sjölin, Amin Niakan, Gholamreza Dabiri, Shahin Mohseni
BACKGROUND: Traumatic brain injury (TBI) is a major cause of death and debility following trauma. The initial brain tissue insult is worsened by secondary reactive responses including oxidative stress reactions, inflammatory changes and subsequent permanent neurologic deficits. Effective agents to improve functional outcome and survival following TBI are scarce. Selenium is an antioxidant which has shown to reduce oxidative stress. This study examines the effect of intravenous selenium (Selenase(®)) treatment in patients with severe TBI on functional outcome and survival in a prospective study design...
July 8, 2017: Injury
https://www.readbyqxmd.com/read/28706545/effect-of-face-to-face-education-problem-based-learning-and-goldstein-systematic-training-model-on-quality-of-life-and-fatigue-among-caregivers-of-patients-with-diabetes
#5
Reza Masoudi, Mohammad Ali Soleimani, Ameneh Yaghoobzadeh, Shahram Baraz, Ashrafalsadat Hakim, Yiong H Chan
BACKGROUND: Education is a fundamental component for patients with diabetes to achieve good glycemic control. In addition, selecting the appropriate method of education is one of the most effective factors in the quality of life. The present study aimed to evaluate the effect of face-to-face education, problem-based learning, and Goldstein systematic training model on the quality of life (QOL) and fatigue among caregivers of patients with diabetes. MATERIALS AND METHODS: This randomized clinical trial was conducted in Hajar Hospital (Shahrekord, Iran) in 2012...
May 2017: Iranian Journal of Nursing and Midwifery Research
https://www.readbyqxmd.com/read/28702120/inpatient-versus-outpatient-management-of-tia-or-minor-stroke-clinical-outcome
#6
Shahram Majidi, Christopher R Leon Guerrero, Kathleen M Burger, John F Rothrock
BACKGROUND: The management of patients with acute transient ischemic attack (TIA) or minor stroke is highly variable. Whether hospitalization of such patients significantly improves short-term clinical outcome is unknown. We assessed the short-term clinical outcome associated with inpatient versus outpatient management of patients with TIA or minor stroke. METHODS: We evaluated a consecutive series of patients with acute TIA or minor ischemic stroke (NIH Stroke Scale score ≤ 3) presenting to a single emergency department (ED)...
June 2017: Journal of Vascular and Interventional Neurology
https://www.readbyqxmd.com/read/28698500/use-of-polycaprolactone-electrospun-nanofibers-as-a-coating-for-poly-methyl-methacrylate-bone-cement
#7
Morshed Khandaker, Shahram Riahinezhad, Harsha G Jamadagni, Tracy L Morris, Alexis V Coles, Melville B Vaughan
Poly(methyl methacrylate) (PMMA) bone cement has limited biocompatibility. Polycaprolactone (PCL) electrospun nanofiber (ENF) has many applications in the biomedical field due to its excellent biocompatibility and degradability. The effect of coating PCL ENF on the surface topography, biocompatibility, and mechanical strength of PMMA bone cement is not currently known. This study is based on the hypothesis that the PCL ENF coating on PMMA will increase PMMA roughness leading to increased biocompatibility without influencing its mechanical properties...
July 10, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28693954/nano-encapsulation-of-chicken-immunoglobulin-igy-in-sodium-alginate-nanoparticles-in%C3%A2-vitro-characterization
#8
Mostafa Bakhshi, Firouz Ebrahimi, Shahram Nazarian, Jamil Zargan, Faeze Behzadi, Dawood Sadeghi Gariz
Controlled delivery of therapeutic agents by alginate nanoparticles became an attractive issue in the gastric organ. Some therapeutic agents such as proteins could not tolerate in severe condition in the gastrointestinal tract. In the present study, four concentrations of a specific IgY as a prophylactic agent against E. coli O157: H7 was entrapped in 0.2% w/v sodium alginate nanoparticles by ionic gelation method. Depending on the IgY concentration entrapment efficacy was 28.31-99.84%. The physicochemical and structural characteristics of free and IgY-loaded Alg NPs revealed that the individual particles exhibited a spherical shape with a diameter of 45-85 nm, and a negatively charged surface with a zeta potential value of 26-36 mV...
July 7, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28691890/mtrr-rs1801394-and-its-interaction-with-mthfr-rs1801133-in-colorectal-cancer-a-case-control-study-and-meta-analysis
#9
Monir Sadat Haerian, Batoul Sadat Haerian, Saadat Molanaei, Farid Kosari, Shahram Sabeti, Farahnaz Bidari-Zerehpoosh, Ebrahim Abdolali
AIM: This study aims to evaluate the association between the MTRR rs1801394 alone or in interaction with the MTHFR rs1801133 and susceptibility to colorectal cancer (CRC) and its characteristics in Iranian population. Additionally, both a systematic review and meta-analysis were performed to derive a more precise assessment of this association. MATERIALS & METHODS: Genomic DNA of 2332 subjects was genotyped for rs1801394. These data were pooled with 17 eligible studies for meta-analysis...
July 10, 2017: Pharmacogenomics
https://www.readbyqxmd.com/read/28688622/effects-of-atorvastatin-on-brain-contusion-volume-and-functional-outcome-of-patients-with-moderate-and-severe-traumatic-brain-injury-a-randomized-double-blind-placebo-controlled-clinical-trial
#10
Gholam Reza Farzanegan, Nima Derakhshan, Hosseinali Khalili, Fariborz Ghaffarpasand, Shahram Paydar
The aim of the current study was to investigate the effects of atorvastatin on brain contusion volume and functional outcome of patients with moderate and severe traumatic brain injury (TBI). The study was conducted as a randomized clinical trial during a 16-month period from May 2015 and August 2016 in a level I trauma center in Shiraz, Southern Iran. We included 65 patients with moderate (GCS: 9-13) to severe (GCS: 5-8) TBI who had brain contusions of less than 30cc volume. We excluded those who required surgical intervention...
July 5, 2017: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
https://www.readbyqxmd.com/read/28687718/mortality-trends-for-multiple-sclerosis-patients-in-sweden-from-1968-to-2012
#11
Sarah Burkill, Scott Montgomery, MohammadHossein Hajiebrahimi, Jan Hillert, Tomas Olsson, Shahram Bahmanyar
OBJECTIVE: To assess trends in mortality and causes of death for patients with multiple sclerosis (MS) relative to those without MS in Sweden. METHODS: Patients with an MS diagnosis in Sweden between 1964 and 2012 were identified with the Patient Register and the Multiple Sclerosis Register. For this cohort study, each patient with MS (n = 29,617) was matched with 10 individuals without MS (n = 296,164) on sex, year of birth, vital status, and region of residence at the time of MS diagnosis with the Total Population Register...
July 7, 2017: Neurology
https://www.readbyqxmd.com/read/28681817/the-evaluation-of-interferon-lambda-4-rs368234815-as-a-predictor-factor-in-treated-patients-with-chronic-hepatitis-c-genotype-1a-infection
#12
Shahram Jalilian, Seyed Mahmoud Latifi, Manoochehr Makvandi, Ali Teimoori, Azarakhsh Azaran, Mehdi Parsanahad, Gholamabas Kayedani
CONTEXT: Host factors including single-nucleotide polymorphisms (SNPs) in or near interferon lambda (IFNL) gene are the important factors in predicting response to treatment of chronic hepatitis C (CHC). AIMS: The aim of this study was to determine the frequency and association of IFNL4 rs368234815 with IFNL3 SNPs rs12979860, rs8099917 and other factors including cholesterol, alanine aminotransferase, fibrosis, viral load, age and body mass index in genotype 1a treated CHC patients, to achieve rapid virologic response (RVR) and sustained virologic response (SVR)...
April 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28680518/the-affordable-care-act-disparities-in-emergency-department-use-for-mental-health-diagnoses-in-young-adults
#13
Justin Yanuck, Bryson Hicks, Craig Anderson, John Billimek, Shahram Lotfipour, Bharath Chakravarthy
BACKGROUND: There is little consensus as to the effects of insurance expansion on emergency department (ED) utilization for mental health purposes. We aimed to study the race specific association between the dependent coverage provision of the Affordable Care Act (ACA) and changes in young adults' usage of emergency department services for psychiatric diagnoses. METHODS: We utilized a Quasi-Experimental analysis of ED use in California from 2009-2011 for behavioral health diagnoses of individuals aged 19 to 31 years...
2017: World Journal of Emergency Medicine
https://www.readbyqxmd.com/read/28679849/mendelian-genes-and-risk-of-intracerebral-hemorrhage-and-small-vessel-ischemic-stroke-in-sporadic-cases
#14
Michael Chong, Martin O'Donnell, Vincent Thijs, Antonio Dans, Patricio López-Jaramillo, Diego Gómez-Arbeláez, Charles Mondo, Anna Czlonkowska, Marta Skowronska, Shahram Oveisgharan, Salim Yusuf, Guillaume Paré
BACKGROUND AND PURPOSE: Mendelian strokes are rare genetic disorders characterized by early-onset small-vessel stroke. Although extensively studied among families with syndromic features, whether these genes affect risk among sporadic cases is unknown. METHODS: We sequenced 8 genes responsible for Mendelian stroke in a case-control study of sporadic stroke cases (≤70 years). Participants included 1251 primary stroke cases of small-vessel pathology (637 intracerebral hemorrhage and 614 small-vessel ischemic stroke cases) and 1716 controls from the INTERSTROKE study (Study of the Importance of Conventional and Emerging Risk Factors of Stroke in Different Regions and Ethnic Groups of the World)...
July 5, 2017: Stroke; a Journal of Cerebral Circulation
https://www.readbyqxmd.com/read/28677982/determinants-of-incident-metabolic-syndrome-in-a-middle-eastern-population-isfahan-cohort-study
#15
Naeimeh Hosseini, Mohammad Talaei, Minoo Dianatkhah, Masoumeh Sadeghi, Shahram Oveisgharan, Nizal Sarrafzadegan
BACKGROUND: To identify the associated risk factors with development of metabolic syndrome (MetS) in a longitudinal prospective cohort study in an Iranian population. METHODS: A total of 1994 participants, aged ≥35 years, free of MetS, diabetes, and cardiovascular disease at baseline were followed up for 7 years. Physical examination, laboratory studies, and interview about lifestyle factors were performed, and MetS was defined based on harmonized definition at both time points...
July 5, 2017: Metabolic Syndrome and related Disorders
https://www.readbyqxmd.com/read/28676592/epidemiologic-trend-of-smear-positive-smear-negative-extra-pulmonary-and-relapse-of-tuberculosis-in-iran-2001-2015-a-repeated-cross-sectional-study
#16
Shahram Arsang-Jang, Marjan Mansourian, Firouz Amani, Tohid Jafari-Koshki
BACKGROUND: Trend analysis is an important tool to monitor epidemiological changes of disease over time to guide resource allocation. This study aimed to study incidence trends and change-points of smear positive, smear negative, extra-pulmonary and relapse of tuberculosis (TB) in Iran from 2001 to 2015. STUDY DESIGN: Repeated cross-sectional study METHODS: Nonlinear segmented regression was used to describe TB incidence trends; annual percent change (APC), average annual percent changes (AAPC) and change points for each disease separately...
May 20, 2017: Journal of Research in Health Sciences
https://www.readbyqxmd.com/read/28676033/the-effects-of-probiotic-bacillus-subtilis-on-the-cytotoxicity-of-clostridium-perfringens-type-a-in-caco-2-cell-culture
#17
Maryam Poormontaseri, Saeid Hosseinzadeh, Seyed Shahram Shekarforoush, Tahereh Kalantari
BACKGROUND: Some Bacillus strains have recently been identified for potential use as probiotics and food additives. The present study evaluated the antimicrobial effects of Bacillus subtilis ATCC 6633 and its metabolite on the enterotoxin and vegetative cells, spore and germinated spore of Clostridium perfringens type A in Caco-2 cells. RESULTS: We used flow cytometry and MTT assays to evaluate the cytotoxicity effect of treatments. According to the results, the most cell survival was found in the 4% crude antimicrobial substance (CAS) with the vegetative form of C...
July 4, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28671883/prevention-of-peritendinous-adhesion-formation-after-the-flexor-tendon-surgery-in-rabbits-a-comparative-study-between-use-of-local-interferon-%C3%AE-interferon-%C3%AE-and-5-fluorouracil
#18
Mohammad Javad Fatemi, Shahram Shirani, Roohollah Sobhani, Amir Hossein Lebaschi, Mohammad Javad Gharegozlou, Tooran Bagheri, Mirsepehr Pedram, Mohsen Saberi, Shirin Araghi, Mohammad Ali Fatemi
BACKGROUND: Peritendinous adhesion is the most common complication after tendon surgery, particularly in zone II of the hand. Prevention of inflammation around the tendon, which develops after trauma and surgery, can decrease the tendon adhesion formation. This study compares the effect of some anti-inflammatory cytokines with 5-fluorouracil (5-FU) on the tensile strength and in prevention of peritendinous adhesion formation. METHODS: Sixteen rabbits were allocated equally into 4 groups...
June 1, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28671710/adult-xanthogranuloma-diagnosed-on-reflectance-confocal-microscopy
#19
Attiya Haroon, Shahram Shafi, Babar Rao
No abstract text is available yet for this article.
July 3, 2017: Journal of Cutaneous Pathology
https://www.readbyqxmd.com/read/28670677/a-randomized-control-trial-comparing-combined-glandular-lidocaine-injection-and-intraurethral-lidocaine-gel-with-intraurethral-lidocaine-gel-alone-in-cystoscopy-and-urethral-dilatation
#20
Shahram Gooran, Pejman Pourfakhr, Shirin Bahrami, Ali Mohammad Fakhr Yasseri, Amir Javid, Negar Behtash, Gholamreza Pourmand
PURPOSE: Cystoscopy is one of the most common urologic procedures. The aim of this study is to investigate the combined effect of intraurethral lidocaine gel and intraglandular injection of lidocaine 2% on pain during and after cystoscopy. Materials & Methods: In this double-blind, parallel group randomized clinical trial, 156 patients referred for double J removal, urethral dilatation, or cystoscopy were enrolled. The patients were divided into two groups, A and B. All patients received 20 cc of intraurethral lidocaine gel 2%...
July 2, 2017: Urology Journal
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