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Vincent Poot, Maaike Hoekstra, Mitchell A A Geleijnse, Mark C M van Loosdrecht, Julio Pérez
Partial nitritation was stably achieved in a bench-scale airlift reactor (1.5L) containing granular sludge. Continuous operation at 20 °C treating low-strength synthetic wastewater (50 mg N-NH4(+)/L and no COD) achieved nitrogen loading rates of 0.8 g N-NH4(+)/(L·d) during partial nitritation. The switch between nitrite-oxidizing bacteria (NOB) repression and NOB proliferation was observed when ammonium concentrations in the reactor were below 2-5 mg N-NH4(+)/L for DO concentrations lower than 4 mg O2/L at 20 °C...
October 11, 2016: Water Research
Neehar D Parikh, Vincent D Marshall, Amit G Singal, Hari Nathan, Anna S Lok, Rajesh Balkrishnan, Vahakn Shahinian
: Sorafenib is the only chemotherapeutic approved for treatment of advanced hepatocellular carcinoma (HCC.) However, its effectiveness in patients with Child Pugh B cirrhosis and any moderating effects of health system characteristics are unclear. We aimed to examine the survival and cost-effectiveness associated with sorafenib in elderly patients with advanced HCC. We performed an analysis of Medicare beneficiaries with HCC diagnoses from 2007-2009. We compared advanced stage patients with HCC (AJCC stage III/IV) who received sorafenib within 6 months of diagnosis (and were otherwise untreated) to advanced stage patients with HCC who received no therapy (control...
October 22, 2016: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
Martine Boccara, Alexis Sarazin, Bernard Billoud, Agnes Bulski, Louise Chapell, David Baulcombe, Vincent Colot
Epigenetic response to stress in plants involves changes in DNA methylation, histone modifications, and expression of small noncoding RNAs (sRNA). Here we present the method of analysis of differential expression of sRNA populations using DNA tiling arrays. sRNA extracted from Arabidopsis thaliana plants exposed to pathogen elicitor or control plants were reverse-transcribed into cDNAs, and subsequently hybridized after labeling to a custom-made DNA tiling array covering Arabidopsis chromosome 4. We first designed a control experiment with eight cDNA clones corresponding to sequences located on chromosome 4 and obtained robust and specific hybridization signals...
2017: Methods in Molecular Biology
Hugo de Almeida, Vincent Leroux, Flávia Nader Motta, Philippe Grellier, Bernard Maigret, Jaime M Santana, Izabela Marques Dourado Bastos
We have previously demonstrated that the secreted prolyl oligopeptidase of Trypanosoma cruzi (POPTc80) is involved in the infection process by facilitating parasite migration through the extracellular matrix. We have built a 3D structural model where POPTc80 is formed by a catalytic α/β-hydrolase domain and a β-propeller domain, and in which the substrate docks at the inter-domain interface, suggesting a "jaw opening" gating access mechanism. This preliminary model was refined by molecular dynamics simulations and next used for a virtual screening campaign, whose predictions were tested by standard binding assays...
October 21, 2016: Journal of Computer-aided Molecular Design
Sofia Morfopoulou, Edward T Mee, Sarah M Connaughton, Julianne R Brown, Kimberly Gilmour, W K 'Kling' Chong, W Paul Duprex, Deborah Ferguson, Mike Hubank, Ciaran Hutchinson, Marios Kaliakatsos, Stephen McQuaid, Simon Paine, Vincent Plagnol, Christopher Ruis, Alex Virasami, Hong Zhan, Thomas S Jacques, Silke Schepelmann, Waseem Qasim, Judith Breuer
Routine childhood vaccination against measles, mumps and rubella has virtually abolished virus-related morbidity and mortality. Notwithstanding this, we describe here devastating neurological complications associated with the detection of live-attenuated mumps virus Jeryl Lynn (MuV(JL5)) in the brain of a child who had undergone successful allogeneic transplantation for severe combined immunodeficiency (SCID). This is the first confirmed report of MuV(JL5) associated with chronic encephalitis and highlights the need to exclude immunodeficient individuals from immunisation with live-attenuated vaccines...
October 21, 2016: Acta Neuropathologica
Na Jin Seo, Mojtaba F Fathi, Pilwon Hur, Vincent Crocher
STUDY DESIGN: Repeated measures. INTRODUCTION: The Kinect (Microsoft, Redmond, WA) is widely used for telerehabilitation applications including rehabilitation games and assessment. PURPOSE OF THE STUDY: To determine effects of the Kinect location relative to a person on measurement accuracy of upper limb joint angles. METHODS: Kinect error was computed as difference in the upper limb joint range of motion (ROM) during target reaching motion, from the Kinect vs 3D Investigator Motion Capture System (NDI, Waterloo, Ontario, Canada), and compared across 9 Kinect locations...
October 18, 2016: Journal of Hand Therapy: Official Journal of the American Society of Hand Therapists
Marion Rouzaire, Aurélie Comptour, Corinne Belville, Damien Bouvier, Gaël Clairefond, Flora Ponelle, Vincent Sapin, Denis Gallot, Loïc Blanchon
Thirty percent of preterm births directly result from preterm premature rupture of fetal membranes (PPROM). Clinical management currently proposes using a collagen plug to mechanically stop loss of amniotic fluid. Vitamin A and its active metabolite (retinoic acid) have well-known pro-healing properties and could thus make good candidates as a proposable adjuvant to this mechanical approach. Here we investigate the molecular mechanisms involved in the pro-healing properties of all-trans retinoic acid (atRA) in fetal membranes via an approach using an in vitro primary amniocyte wound model and transcriptomics...
October 18, 2016: International Journal of Biochemistry & Cell Biology
Yuanyuan Wu, Vincent T Remcho
Capillary electrophoresis (CE) coupled with UV absorbance detection has been applied as an analysis tool for carbon dots (C-dots), which were hydrothermally synthesized from precursors citric acid (CA) and ethylene diamine (EDA). An alkaline working buffer (100mM tris acetate, pH 8.4) was found to be optimal for rapid and reliable analysis of C-dots. A calibration curve was established over a broad concentration range (0.5-10mg/mL) with excellent linearity (R(2)=0.9989). The CE method was also applied to enhance understanding of and facilitate optimization of ensuing C-dot biolabeling reactions...
December 1, 2016: Talanta
Vincent K Dik, Ignace T C Hooge, Martijn G H van Oijen, Peter D Siersema
BACKGROUND AND OBJECTIVE: Considerable variation in adenoma detection has been shown between endoscopists, which may be explained by differences in colon inspection. Eye-tracking technology is an objective tool that detects differences in viewing patterns. We investigated the feasibility of eye-tracking technology during real-time, self-performed colonoscopies. METHODS: In this pilot study, 10 endoscopists performed two colonoscopies each. A mobile eye-tracking system to register the right eye position was used to determine the gaze across four areas of interest of the endoscopy monitor (upper, lower, left, and right quadrant)...
December 2016: European Journal of Gastroenterology & Hepatology
Heba M Ismail, Athanasios Didangelos, Tonia L Vincent, Jeremy Saklatvala
Objectives Mechanical injury to cartilage predisposes to osteoarthritis (OA). Wounding the articular cartilage surface causes rapid activation of MAP kinases and NFκB mimicking the response to inflammatory cytokines. Here, we identify the upstream signalling mechanisms involved. Methods Cartilage was injured by dissecting it from the articular surface of porcine metacarpophalangeal joints (MCP) or by avulsing murine proximal femoral epiphyses. Protein phosphorylation was assayed by Western blotting of cartilage lysates...
October 21, 2016: Arthritis & Rheumatology
Philippe Hermand, Annick Vandercammen, Emmanuel Mertens, Emmanuel Di Paolo, Vincent Verlant, Philippe Denoël, Fabrice Godfroid
The use of protein antigens able to protect against the majority of Streptococcus pneumoniae serotypes is envisaged as stand-alone and/or complement to the current capsular polysaccharide-based pneumococcal vaccines. Pneumolysin (Ply) is a key virulence factor that is highly conserved in amino acid sequence across pneumococcal serotypes, and therefore may be considered as a vaccine target. However, native Ply cannot be used in vaccines due to its intrinsic cytolytic activity. In the present work a completely, irreversibly detoxified pneumolysin (dPly) has been generated using an optimized formaldehyde treatment...
October 21, 2016: Human Vaccines & Immunotherapeutics
H Abdalla, A Abramowski, F Aharonian, F Ait Benkhali, A G Akhperjanian, T Andersson, E O Angüner, M Arrieta, P Aubert, M Backes, A Balzer, M Barnard, Y Becherini, J Becker Tjus, D Berge, S Bernhard, K Bernlöhr, E Birsin, R Blackwell, M Böttcher, C Boisson, J Bolmont, P Bordas, J Bregeon, F Brun, P Brun, M Bryan, T Bulik, M Capasso, J Carr, S Casanova, N Chakraborty, R Chalme-Calvet, R C G Chaves, A Chen, J Chevalier, M Chrétien, S Colafrancesco, G Cologna, B Condon, J Conrad, C Couturier, Y Cui, I D Davids, B Degrange, C Deil, J Devin, P deWilt, A Djannati-Ataï, W Domainko, A Donath, L O'C Drury, G Dubus, K Dutson, J Dyks, M Dyrda, T Edwards, K Egberts, P Eger, J-P Ernenwein, S Eschbach, C Farnier, S Fegan, M V Fernandes, A Fiasson, G Fontaine, A Förster, S Funk, M Füßling, S Gabici, M Gajdus, Y A Gallant, T Garrigoux, G Giavitto, B Giebels, J F Glicenstein, D Gottschall, A Goyal, M-H Grondin, M Grudzińska, D Hadasch, J Hahn, J Hawkes, G Heinzelmann, G Henri, G Hermann, O Hervet, A Hillert, J A Hinton, W Hofmann, C Hoischen, M Holler, D Horns, A Ivascenko, A Jacholkowska, M Jamrozy, M Janiak, D Jankowsky, F Jankowsky, M Jingo, T Jogler, L Jouvin, I Jung-Richardt, M A Kastendieck, K Katarzyński, U Katz, D Kerszberg, B Khélifi, M Kieffer, J King, S Klepser, D Klochkov, W Kluźniak, D Kolitzus, Nu Komin, K Kosack, S Krakau, M Kraus, F Krayzel, P P Krüger, H Laffon, G Lamanna, J Lau, J-P Lees, J Lefaucheur, V Lefranc, A Lemière, M Lemoine-Goumard, J-P Lenain, E Leser, R Liu, T Lohse, M Lorentz, I Lypova, V Marandon, A Marcowith, C Mariaud, R Marx, G Maurin, N Maxted, M Mayer, P J Meintjes, M Meyer, A M W Mitchell, R Moderski, M Mohamed, K Morå, E Moulin, T Murach, M de Naurois, F Niederwanger, J Niemiec, L Oakes, P O'Brien, H Odaka, S Ohm, M Ostrowski, S Öttl, I Oya, M Padovani, M Panter, R D Parsons, M Paz Arribas, N W Pekeur, G Pelletier, C Perennes, P-O Petrucci, B Peyaud, S Pita, H Poon, D Prokhorov, H Prokoph, G Pühlhofer, M Punch, A Quirrenbach, S Raab, A Reimer, O Reimer, M Renaud, R de Los Reyes, F Rieger, C Romoli, S Rosier-Lees, G Rowell, B Rudak, C B Rulten, V Sahakian, D Salek, D A Sanchez, A Santangelo, M Sasaki, R Schlickeiser, F Schüssler, A Schulz, U Schwanke, S Schwemmer, M Settimo, A S Seyffert, N Shafi, I Shilon, R Simoni, H Sol, F Spanier, G Spengler, F Spies, Ł Stawarz, R Steenkamp, C Stegmann, F Stinzing, K Stycz, I Sushch, J-P Tavernet, T Tavernier, A M Taylor, R Terrier, L Tibaldo, M Tluczykont, C Trichard, R Tuffs, J van der Walt, C van Eldik, B van Soelen, G Vasileiadis, J Veh, C Venter, A Viana, P Vincent, J Vink, F Voisin, H J Völk, T Vuillaume, Z Wadiasingh, S J Wagner, P Wagner, R M Wagner, R White, A Wierzcholska, P Willmann, A Wörnlein, D Wouters, R Yang, V Zabalza, D Zaborov, M Zacharias, A A Zdziarski, A Zech, F Zefi, A Ziegler, N Żywucka
: A search for dark matter linelike signals iss performed in the vicinity of the Galactic Center by the H.E.S.S. experiment on observational data taken in 2014. An unbinned likelihood analysis iss developed to improve the sensitivity to linelike signals. The upgraded analysis along with newer data extend the energy coverage of the previous measurement down to 100 GeV. The 18 h of data collected with the H.E.S.S. array allow one to rule out at 95% C.L. the presence of a 130 GeV line (at l=-1...
October 7, 2016: Physical Review Letters
Ashton A Connor, Robert E Denroche, Gun Ho Jang, Lee Timms, Sangeetha N Kalimuthu, Iris Selander, Treasa McPherson, Gavin W Wilson, Michelle A Chan-Seng-Yue, Ivan Borozan, Vincent Ferretti, Robert C Grant, Ilinca M Lungu, Eithne Costello, William Greenhalf, Daniel Palmer, Paula Ghaneh, John P Neoptolemos, Markus Buchler, Gloria Petersen, Sarah Thayer, Michael A Hollingsworth, Alana Sherker, Daniel Durocher, Neesha Dhani, David Hedley, Stefano Serra, Aaron Pollett, Michael H A Roehrl, Prashant Bavi, John M S Bartlett, Sean Cleary, Julie M Wilson, Ludmil B Alexandrov, Malcolm Moore, Bradly G Wouters, John D McPherson, Faiyaz Notta, Lincoln D Stein, Steven Gallinger
Importance: Outcomes for patients with pancreatic ductal adenocarcinoma (PDAC) remain poor. Advances in next-generation sequencing provide a route to therapeutic approaches, and integrating DNA and RNA analysis with clinicopathologic data may be a crucial step toward personalized treatment strategies for this disease. Objective: To classify PDAC according to distinct mutational processes, and explore their clinical significance. Design, Setting, and Participants: We performed a retrospective cohort study of resected PDAC, using cases collected between 2008 and 2015 as part of the International Cancer Genome Consortium...
October 20, 2016: JAMA Oncology
Deborah Brazzolotto, Marcello Gennari, Nicolas Queyriaux, Trevor R Simmons, Jacques Pécaut, Serhiy Demeshko, Franc Meyer, Maylis Orio, Vincent Artero, Carole Duboc
Hydrogen production through water splitting is one of the most promising solutions for the storage of renewable energy. [NiFe] hydrogenases are organometallic enzymes containing nickel and iron centres that catalyse hydrogen evolution with performances that rival those of platinum. These enzymes provide inspiration for the design of new molecular catalysts that do not require precious metals. However, all heterodinuclear NiFe models reported so far do not reproduce the Ni-centred reactivity found at the active site of [NiFe] hydrogenases...
November 2016: Nature Chemistry
Juntian Zhang, Dawen Niu, Vincent A Brinker, Thomas R Hoye
Benzynes produced thermally by the cycloisomerization of triyne-containing precursors [i.e., by the hexadehydro-Diels-Alder (HDDA) reaction] react with phenols at the carbon ortho to the hydroxyl in an enelike fashion. Following tautomerization of the intermediate cyclohexadienones, this produces biaryl derivatives. DFT calculations of model reactions support this mechanistic interpretation. Substituted, unsymmetrical phenols and bis-phenols react in a fashion that can be explained by engagement of the most readily available (non-hydrogen-bonded) hydroxyl in the phenol-ene process...
October 21, 2016: Organic Letters
Ke Yuan, Sang Soo Lee, Vincent De Andrade, Neil C Sturchio, Paul Fenter
The mobility of toxic elements, such as lead (Pb) can be attenuated by adsorption, incorporation, and precipitation on carbonate minerals in subsurface environments. Here, we report a study of the bulk transformation of single-crystal calcite (CaCO3) into polycrystalline cerussite (PbCO3) through reaction with acidic Pb-bearing solutions. This reaction began with the growth of a cerussite shell on top of calcite surfaces followed by the replacement of the remaining calcite core. The external shape of the original calcite was preserved by a balance between calcite dissolution and cerussite growth controlled by adjusting the Pb(2)(+) concentration and pH...
October 21, 2016: Environmental Science & Technology
Ulysse Gimenez, Hélène Lajous, Michèle El Atifi, Marie Bidart, Vincent Auboiroux, Pascal Henry Fries, François Berger, Hana Lahrech
Cellular MRI, which visualizes magnetically labelled cells (cells*), is an active research field for in vivo cell therapy and tracking. The simultaneous relaxation rate measurements (R2 *, R2 , R1 ) are the basis of a quantitative cellular MRI method proposed here. U937 cells were labelled with Molday ION Rhodamine B, a bi-functional superparamagnetic and fluorescent nanoparticle (U937*). U937* viability and proliferation were not affected in vitro. In vitro relaxometry was performed in a cell concentration range of [2...
October 21, 2016: Contrast Media & Molecular Imaging
Momotazur Rahman, David C Grabowski, Vincent Mor, Edward C Norton
OBJECTIVE: To determine whether the observed differences in the risk-adjusted rehospitalization rates across skilled nursing facilities (SNFs) reflect true differences or merely differences in patient severity. SETTINGS: Elderly Medicare beneficiaries newly admitted to an SNF following hospitalization. STUDY DESIGN: We used 2009-2012 Medicare data to calculate SNFs' risk-adjusted rehospitalization rate. We then estimated the effect of these rehospitalization rates on the rehospitalization of incident patients in 2013, using an instrumental variable (IV) method and controlling for patient's demographic and clinical characteristics and residential zip code fixed effects...
October 21, 2016: Health Services Research
Vincent Yi-Fong Su, Chia-Jen Liu, Yuh-Min Chen, Teh-Ying Chou, Tzeng-Ji Chen, Sang-Hue Yen, Tzeon-Jye Chiou, Jin-Hwang Liu, Yu-Wen Hu
BACKGROUND: Currently, no large study addressing the relationship between lung cancer patients with different therapies and second primary malignancies (SPMs) is available. METHODS: Using the Taiwan National Health Insurance Research Database, we conducted a population-based cohort study. Patients with newly diagnosed lung cancer between 1997 and 2005 were enrolled and followed up until Dec. 31, 2011. The endpoint of the study was SPM occurrence. Standardized incidence ratios (SIRs) of cancers were calculated to compare the cancer incidence of the study cohort to that of the general population...
October 20, 2016: Targeted Oncology
Benoit Peyronnet, Grégoire Robert, Vincent Comat, Morgan Rouprêt, Fernando Gomez-Sancha, Jean-Nicolas Cornu, Vincent Misrai
PURPOSE: To compare the learning curves, perioperative and early functional outcomes after HoLEP and GreenLEP. METHODS: Data from the first 100 consecutive cases treated by GreenLEP and HoLEP by two surgeons were prospectively collected from dedicated databases and analysed retrospectively. En-bloc GreenLEP and two-lobar HoLEP enucleations were conducted using the GreenLight HPS™ 2090 laser and Lumenis™ holmium laser. Patients' characteristics, perioperative outcomes and functional outcomes after 1, 3 and 6 months were compared between groups...
October 20, 2016: World Journal of Urology
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