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https://www.readbyqxmd.com/read/28812170/a-novel-approach-to-utilize-icariin-as-icariin-derived-ecm-on-small-intestinal-submucosa-scaffold-for-bone-repair
#1
Mei Li, Chi Zhang, Yi Zhong, Jiyuan Zhao
Icariin (Ic) has been demonstrated as a potent osteoinductive compound for bone tissue engineering. However, toxic side effects of the drug and poor biocompatibility of drug delivery systems (DDSs) still limit its application for bone repair in the clinic. To overcome these disadvantages and utilize the osteoinductivity of Ic, we developed a novel method to utilize Ic as an Ic-derived osteoinductive extracellular matrix (ECM) on small intestinal submucosa (SIS) (Ic-ECM-SIS). The generated Ic-ECM-SIS scaffolds, as a natural construct, exhibited much better biocompatibility (including cell adhesion, cell survival and cell proliferation) than Ic-SIS scaffolds generated by traditional DDSs...
August 15, 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/28811575/biological-and-functional-characterization-of-bone-marrow-derived-mesenchymal-stromal-cells-from-patients-affected-by-primary-immunodeficiency
#2
Nadia Starc, Daniela Ingo, Antonella Conforti, Valeria Rossella, Luigi Tomao, Angela Pitisci, Fabiola De Mattia, Immacolata Brigida, Mattia Algeri, Mauro Montanari, Giuseppe Palumbo, Pietro Merli, Paolo Rossi, Alessandro Aiuti, Franco Locatelli, Maria Ester Bernardo
Mesenchymal stromal cells (MSCs) represent a key component of bone marrow (BM) microenvironment and display immune-regulatory properties. We performed a detailed analysis of biological/functional properties of BM-MSCs derived from 33 pediatric patients affected by primary immune-deficiencies (PID-MSCs): 7 Chronic Granulomatous Disease (CGD), 15 Wiskott-Aldrich Syndrome (WAS), 11 Severe Combined Immunodeficiency (SCID). Results were compared with MSCs from 15 age-matched pediatric healthy-donors (HD-MSCs). Clonogenic and proliferative capacity, differentiation ability, immunophenotype, immunomodulatory properties were analyzed...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28811183/deletion-of-protein-kinase-d1-in-osteoprogenitor-cells-results-in-decreased-osteogenesis-in-vitro-and-reduced-bone-mineral-density-in-vivo
#3
Wendy B Bollag, Vivek Choudhary, Qing Zhong, Ke-Hong Ding, Jianrui Xu, Ranya Elsayed, Kanglun Yu, Yun Su, Lakiea J Bailey, Xing-Ming Shi, Mohammed Elsalanty, Maribeth H Johnson, Meghan E McGee-Lawrence, Carlos M Isales
Protein kinase D1 (PRKD1) is thought to play a role in a number of cellular functions, including proliferation and differentiation. We hypothesized that PRKD1 in bone marrow-derived mesenchymal stem cells (BMMSC) could modulate osteogenesis. In BMMSCs from floxed PRKD1 mice, PRKD1 ablation with adenovirus-mediated Cre-recombinase expression inhibited BMMSC differentiation in vitro. In 3- and 6-month-old conditional knockout mice (cKO), in which PRKD1 was ablated in osteoprogenitor cells by osterix promoter-driven Cre-recombinase, bone mineral density (BMD) was significantly reduced compared with floxed control littermates...
August 12, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28809642/fibular-strut-graft-for-posterior-pelvic-ring-nonunion-a-case-report
#4
M Frank, J R McCallum, T Dědek
Posttraumatic pelvic nonunions in combination with malposition are uncommon in the present-day era of modern pelvic surgery. The case describes a new surgical technique for treatment of the nonunion localized to iliosacral joint. A 42-year-old polytraumatized male presented with a pelvic fracture (type 61-C2.3) associated with a complex acetabular fracture. The patient was treated and the pelvis stabilized according to damage control principles (external fixation and pelvic C-clamp) and subsequently definitively treated according to principles of current pelvic surgery...
2017: Acta Chirurgiae Orthopaedicae et Traumatologiae Cechoslovaca
https://www.readbyqxmd.com/read/28808627/non-ossifying-fibromas-case-series-including-in-uncommon-upper-extremity-sites
#5
Akio Sakamoto, Ryuzo Arai, Takeshi Okamoto, Shuichi Matsuda
AIM: To investigate non-ossifying fibromas (NOFs) common fibrous bone lesions in children that occur in bones of the lower extremities. METHODS: We analyzed 44 cases of NOF including 47 lesions, which were referred with a working diagnosis of neoplastic lesions. Lesions were located in the upper extremities (1 proximal humerus, 1 distal radius) and the lower extremities (25 distal femurs, 12 proximal and 4 distal tibias, and 4 proximal fibulas). RESULTS: Three cases had NOFs in multiple anatomical locations (femur and fibula in 1 case, femur and tibia in 2 cases)...
July 18, 2017: World Journal of Orthopedics
https://www.readbyqxmd.com/read/28808626/acetabular-revisions-using-porous-tantalum-components-a-retrospective-study-with-5-10-years-follow-up
#6
Francesco Roberto Evola, Luciano Costarella, Giuseppe Evola, Martina Barchitta, Antonella Agodi, Giuseppe Sessa
AIM: To evaluate the clinical and X-ray results of acetabular components and tantalum augments in prosthetic hip revisions. METHODS: Fifty-eight hip prostheses with primary failure of the acetabular component were reviewed with tantalum implants. The clinical records and X-rays of these cases were retrospectively reviewed. Bone defect evaluations were based on preoperative CT scans and classified according to Paprosky criteria of Radiolucent lines and periprosthetic gaps; implant mobilization and osteolysis were evaluated by X-ray...
July 18, 2017: World Journal of Orthopedics
https://www.readbyqxmd.com/read/28808593/combined-bone-transportation-and-lengthening-techniques-for-the-treatment-of-septic-nonunion-of-the-forearm-followed-by-tendon-transfer
#7
Konstantinos Ditsios, Eirini Iosifidou, Lazaros Kostretzis, Panagiotis Konstantinou, Iosafat Pinto, Ioannis Theodoroudis, Ippokratis Hatzokos
Infected nonunion of a forearm fracture complicated by a considerable skin-muscle defect poses a great challenge to orthopaedic surgeons. The treatment strategy comprises eradication of the infection, ensuring bony union and soft tissue coverage along with functional restoration. We report a case of a 23-year-old man with an open Gustilo-Anderson IIIb fracture complicated by infected nonunion after internal fixation. After thorough surgical debridement, a considerable soft tissue defect, extensor muscle loss, and posterior interosseous nerve laceration had to be addressed...
2017: Case Reports in Orthopedics
https://www.readbyqxmd.com/read/28808306/phf20-positively-regulates-osteoblast-differentiation-via-increasing-the-expression-and-activation-of-runx2-with-enrichment-of-h3k4me3
#8
Jin-Woo Yang, Byung-Chul Jeong, Jongsun Park, Jeong-Tae Koh
Plant homeodomain finger protein 20 (PHF20), a methyl lysine effector protein, is a component MOF-NSL lysine acetyltranferase complex. Global deletion of PHF20 has shown spinal bone defects and reduced skeletal formation. However, the molecular basis of PHF20 involved in skeletal development has not been elucidated yet. The objective of this study was to determine the role of PHF20 in osteoblast differentiation and mineralization. Expression of PHF20 was gradually increased during osteoblast differentiation...
August 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28807800/the-synergistic-effects-of-sr-and-si-bioactive-ions-on-osteogenesis-osteoclastogenesis-and-angiogenesis-for-osteoporotic-bone-regeneration
#9
Lixia Mao, Lunguo Xia, Jiang Chang, Jiaqiang Liu, Lingyong Jiang, Chengtie Wu, Bing Fang
Bioactive ions released from bioceramics play important roles in bone regeneration; however, it is unclear how each ionic composition in complex bioceramics exerts its specific effect on bone regeneration. The aim of this study is to elucidate the functional effects of Sr and Si ions in bioceramics on the regeneration of osteoporotic bone. A model bioceramic with Sr- and Si-containing components (SMS) was successfully fabricated and the effects of ionic products from SMS bioceramics on the osteogenic, osteoclastic and angiogenic differentiation of rBMSCs-OVX and RANKL-induced osteoclasts were investigated...
August 11, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28807504/bone-up-craniomandibular-development-and-hard-tissue-biomineralization-in-neonate-mice
#10
Khari D Thompson, Holly E Weiss-Bilka, Elizabeth B McGough, Matthew J Ravosa
The presence of regional variation in the osteogenic abilities of cranial bones underscores the fact that the mechanobiology of the mammalian skull is more complex than previously recognized. However, the relationship between patterns of cranial bone formation and biomineralization remains incompletely understood. In four strains of mice, micro-computed tomography was used to measure tissue mineral density during perinatal development in three skull regions (calvarium, basicranium, mandible) noted for variation in loading environment, embryological origin, and ossification mode...
January 29, 2017: Zoology: Analysis of Complex Systems, ZACS
https://www.readbyqxmd.com/read/28807253/interdisciplinary-reconstruction-of-oncological-resections-at-the-skull-base-scalp-and-facial-region
#11
REVIEW
P M Vogt, T R Mett, G F Broelsch, C Radtke, N-C Gellrich, J K Krauss, M Samii, R Ipaktchi
Extensive bone and soft tissue defects of the skull require unique plastic and reconstructive techniques to avoid and optimize aesthetic appearance following oncological resections. Procedures are ideally planned in an interdisciplinary board composed of neurosurgery, otorhinolaryngology, plastic and reconstructive surgery to facilitate reconstructive procedures of soft tissue and bone as early as possible in one approach. Large resections may require plastic surgery involvement from the beginning. Herein, we describe the function of a multidisciplinary approach to complex oncologic resections of the cranial base, scalp, and facial regions for neuro-oncologic patients...
September 2017: Surgical Oncology
https://www.readbyqxmd.com/read/28806146/three-dimensional-bioprinting-of-polycaprolactone-reinforced-gene-activated-bioinks-for-bone-tissue-engineering
#12
Gráinne M Cunniffe, Tomas Gonzalez-Fernandez, Andrew Daly, Binulal N Sathy, Oju Jeon, Eben Alsberg, Daniel J Kelly
Regeneration of complex bone defects remains a significant clinical challenge. Multi-tool biofabrication has permitted the combination of various biomaterials to create multifaceted composites with tailorable mechanical properties and spatially controlled biological function. In this study we sought to use bioprinting to engineer nonviral gene activated constructs reinforced by polymeric micro-filaments. A gene activated bioink was developed using RGD-γ-irradiated alginate and nano-hydroxyapatite (nHA) complexed to plasmid DNA (pDNA)...
August 14, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28805958/large-orbital-defect-reconstruction-in-the-setting-of-globe-sparing-maxillectomy-the-titanium-hammock-and-layered-fibula-technique
#13
Samuel J Trosman, Timothy M Haffey, Rafael A Couto, Michael A Fritz
INTRODUCTION: The purpose of our study was to describe a novel technique for reconstruction of orbital defects after maxillectomy using a non-anatomic titanium mesh suspension of orbital contents for both support and volume correction. This construct is then articulated with a layered fibula osteocutaneous free flap that restores orbital rim, zygoma, and maxillary alveolus. We herein present our application of this technique, including refinements over time and long-term outcomes. METHODS: A retrospective review was performed on 12 patients who underwent reconstruction of Brown class III orbitopalatomaxillary defects with extensive orbital involvement (at minimum complete orbital floor and rim absent) with titanium mesh sling and a layered fibula free flap...
August 14, 2017: Microsurgery
https://www.readbyqxmd.com/read/28804984/3d-bioprinting-for-cartilage-and-osteochondral-tissue-engineering
#14
REVIEW
Andrew C Daly, Fiona E Freeman, Tomas Gonzalez-Fernandez, Susan E Critchley, Jessica Nulty, Daniel J Kelly
Significant progress has been made in the field of cartilage and bone tissue engineering over the last two decades. As a result, there is real promise that strategies to regenerate rather than replace damaged or diseased bones and joints will one day reach the clinic however, a number of major challenges must still be addressed before this becomes a reality. These include vascularization in the context of large bone defect repair, engineering complex gradients for bone-soft tissue interface regeneration and recapitulating the stratified zonal architecture present in many adult tissues such as articular cartilage...
August 14, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28804813/a-systematic-review-of-the-clinical-applications-and-complications-of-bone-marrow-aspirate-concentrate-in-management-of-bone-defects-and-nonunions
#15
REVIEW
Mohamed A Imam, James Holton, Lukas Ernstbrunner, Wojciech Pepke, Florian Grubhofer, Ali Narvani, Martyn Snow
PURPOSE: Fracture healing encompasses a succession of dynamic multifactorial metabolic events, which ultimately re-establishes the integrity of the biomechanical properties of the bone. Up to 10% of the fractures occurring annually will need additional surgical procedures because of impaired healing. The aim of this article is to review the current literature regarding the use of bone marrow aspirate concentrate (BMAC) and its effectiveness in the management of bone defects. METHODS: We have included all published clinical literature investigating the development, techniques and applications of BMAC...
August 13, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28804668/impaired-mrna-expression-of-the-migration-related-chemokine-receptor-cxcr4-in-mesenchymal-stem-cells-of-copd-patients
#16
K Karagiannis, A Proklou, E Tsitoura, I Lasithiotaki, C Kalpadaki, D Moraitaki, I Sperelakis, G Kontakis, K M Antoniou, N Tzanakis
Defective tissue repair and remodeling are main aspects of Chronic Obstructive Pulmonary Disease (COPD) pathophysiology. Bone marrow mesenchymal stem cells (BM-MSCs) have been implicated in this direction, as their functional impairment and recruitment could possibly contribute to disease development and progression. The present study characterizes for the first time the expression of migration related chemokine receptors and their ligands in BM-MSCs from COPD patients. CXCR4/SDF1a and CCR7/CCL19-CCL21 mRNA levels were evaluated in BM-MSCs obtained from twelve COPD patients and seven healthy donors...
2017: International Journal of Inflammation
https://www.readbyqxmd.com/read/28804562/premature-exhaustion-of-mesenchymal-stromal-cells-from-myelodysplastic-syndrome-patients
#17
Yanbin Pang, Chengxin Deng, Suxia Geng, Jianyu Weng, Peilong Lai, Pengjun Liao, Lingji Zeng, Zesheng Lu, Jing Zhang, Xin Du
Myelodysplastic syndrome (MDS) predominantly occurs in aging people. Over the past decades, the cellular and molecular pathologies of MDS cells have been intensively investigated. However, how the bone marrow stromal niches are altered during MDS development remains elusive. In this study, we attempted to isolate and characterize mesenchymal stromal cells (MSCs) from 30 MDS patients. We observed that only 9/30 bone marrow aspirations from MDS patients successfully formed a monolayer in vitro, while 17/17 bone marrow aspirations from normal donors (median age 45 years, range: 22-73 years) succeeded in this process...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28804540/restoration-of-bone-defects-using-modified-heterogeneous-deproteinized-bone-seeded-with-bone-marrow-mesenchymal-stem-cells
#18
Jun Li, Zeyu Huang, Liyan Chen, Xin Tang, Yue Fang, Lei Liu
The aim of the present study was to investigate the effect of modified heterogeneous deproteinized bone combined with bone marrow mesenchymal stem cells (BMSCs) in the restoration of a validated bone defect model. BMSCs were identified by flow cytometry and multilineage differentiation assay. The structural features of the modified heterogeneous deproteinized bone scaffold and biocompatibility between BMSCs and the scaffold were confirmed by scanning electron microscope (SEM) detection. The cytotoxicity of the modified heterogeneous deproteinized bone scaffolds were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenytetrazolium bromide (MTT) assay...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28803814/revision-total-hip-arthroplasty-with-a-monoblock-splined-tapered-grit-blasted-titanium-stem
#19
Michael D Hellman, Sean M Kearns, Daniel D Bohl, Bryan D Haughom, Brett R Levine
BACKGROUND: In revision total hip arthroplasty (THA), proximal femoral bone loss creates a challenge of achieving adequate stem fixation. The purpose of this study was to examine the outcomes of a monoblock, splined, tapered femoral stem in revision THA. METHODS: Outcomes of revision THA using a nonmodular, splined, tapered femoral stem from a single surgeon were reviewed. With a minimum of 2-year follow-up, there were 68 cases (67 patients). Paprosky classification was 3A or greater in 85% of the cases...
July 5, 2017: Journal of Arthroplasty
https://www.readbyqxmd.com/read/28803246/biofunctional-ionic-doped-calcium-phosphates-silk-fibroin-composites-for-bone-tissue-engineering-scaffolding
#20
S Pina, R F Canadas, G Jiménez, M Perán, J A Marchal, R L Reis, J M Oliveira
The treatment and regeneration of bone defects caused by traumatism or diseases have not been completely addressed by current therapies. Lately, advanced tools and technologies have been successfully developed for bone tissue regeneration. Functional scaffolding materials such as biopolymers and bioresorbable fillers have gained particular attention, owing to their ability to promote cell adhesion, proliferation, and extracellular matrix production, which promote new bone growth. Here, we present novel biofunctional scaffolds for bone regeneration composed of silk fibroin (SF) and β-tricalcium phosphate (β-TCP) and incorporating Sr, Zn, and Mn, which were successfully developed using salt-leaching followed by a freeze-drying technique...
August 12, 2017: Cells, Tissues, Organs
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