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https://www.readbyqxmd.com/read/28545065/influence-of-the-ferric-uptake-regulator-fur-protein-on-pathogenicity-in-pectobacterium-carotovorum-subsp-brasiliense
#1
Collins Kipngetich Tanui, Divine Yutefar Shyntum, Stefan Louis Priem, Jacques Theron, Lucy Novungayo Moleleki
Iron is an important nutrient for the survival and growth of many organisms. In order to survive, iron uptake from the environment must be strictly regulated and maintained to avoid iron toxicity. The ferric uptake regulator protein (Fur) regulates genes involved in iron homeostasis in many bacteria, including phytopathogens. However, to date, the role played by Fur in the biology of Pectobacterium carotovorum subsp. brasiliense (Pcb1692), an important pathogen of potatoes, has not yet been studied. To this end, we used the lambda recombineering method to generate a fur mutant strain of Pcb1692 and assessed the virulence and fitness of the mutant strain...
2017: PloS One
https://www.readbyqxmd.com/read/28544270/localization-and-domain-characterization-of-the-sfla-regulator-of-flagellar-formation-in-vibrio-alginolyticus
#2
Satoshi Inaba, Takehiko Nishigaki, Norihiro Takekawa, Seiji Kojima, Michio Homma
Many swimming bacteria use flagella as locomotive organelles. The spatial and numerical regulation of flagellar biosynthesis differs by bacterial species. The marine bacteria Vibrio alginolyticus use a single polar flagellum whose number is regulated positively by FlhF and negatively by FlhG. Cells lacking FlhF and FlhG have no flagellum. The motility defect in an flhFG deletion was suppressed by a mutation in the sflA gene that resulted in the production of multiple, peritrichous flagella. SflA is a Vibrio-specific protein...
May 22, 2017: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
https://www.readbyqxmd.com/read/28543274/diel-and-seasonal-movements-of-silver-eels-anguilla-dieffenbachii-emigrating-from-a-lake-subject-to-hydro-electric-control
#3
D J Jellyman, M J Unwin
To investigate the behaviour and fate of silver female longfin eels Anguilla dieffenbachii in relation to flow variations from New Zealand's largest (700 MW) hydro-electric station, 210 A. dieffenbachii were tagged with acoustic transmitters during summer and autumn over 6 years from 2005 to 2010. A shoreline array of receivers (2005-2008) was supplemented by positioning systems near the power station and natural outlet (2009-2010) that enabled continuous tracking of A. dieffenbachii within these areas...
May 22, 2017: Journal of Fish Biology
https://www.readbyqxmd.com/read/28542077/physical-activity-sedentary-time-and-risk-of-colorectal-cancer-the-singapore-chinese-health-study
#4
Yvonne L Eaglehouse, Woon-Puay Koh, Renwei Wang, Jin Aizhen, Jian-Min Yuan, Lesley M Butler
Singapore has experienced a marked increase in colorectal cancer incidence over the past 40 years. Evidence from prospective studies in Western Europe and the USA suggests that low physical activity and high amounts of sedentary time are associated with increased colorectal cancer risk. The aim of this study is to evaluate these relationships in an Asian population. The Singapore Chinese Health Study enrolled 63 257 adults between 1993 and 1998. At enrollment, participants reported past year physical activity and time spent sitting...
May 24, 2017: European Journal of Cancer Prevention
https://www.readbyqxmd.com/read/28542004/do-dynamic-fat-and-fat-free-mass-changes-follow-theoretical-driven-rules-in-athletes
#5
Analiza M Silva, Catarina N Matias, Diana A Santos, Paulo M Rocha, Cláudia S Minderico, Diana Thomas, Steven B Heymsfield, Luís B Sardinha
INTRODUCTION: Maximizing fat mass (FM) loss while preserving or increasing fat-free mass (FFM) is a central goal for athletic performance but the composition of body weight (BW) changes over time with training are largely unknown. PURPOSE: We aimed to analyze FM and FFM contributions to BW changes and to test if these contributions follow established rules and predictions over one athletic season. METHODS: 70 athletes (42 males; handball, volleyball, basketball, triathlon, and swimming) were evaluated from the beginning to the competitive stage of the season and were empirically divided into those who lost (n=20) or gained >1...
May 23, 2017: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/28541889/distinct-responses-of-purkinje-neurons-and-roles-of-simple-spikes-during-associative-motor-learning-in-larval-zebrafish
#6
Thomas C Harmon, Uri Magaram, David L McLean, Indira M Raman
To study cerebellar activity during learning, we made whole-cell recordings from larval zebrafish Purkinje cells while monitoring fictive swimming during associative conditioning. Fish learned to swim in response to visual stimulation preceding tactile stimulation of the tail. Learning was abolished by cerebellar ablation. All Purkinje cells showed task-related activity. Based on how many complex spikes emerged during learned swimming, they were classified as multiple, single, or zero complex spike (MCS, SCS, ZCS) cells...
May 25, 2017: ELife
https://www.readbyqxmd.com/read/28540659/neuroprotective-benefits-of-aerobic-exercise-and-organoselenium-dietary-supplementation-in-hippocampus-of-old-rats
#7
José L Cechella, Marlon R Leite, Simone Pinton, Gilson Zeni, Cristina W Nogueira
The progressive decline of neurological functions, such as learning and memory, is an unavoidable consequence of aging. Our previous work suggested that the combination of physical exercise and a diet supplemented with diphenyl diselenide improves age-related memory decline in rats. The present study investigated the effects of physical exercise and a diet supplemented with diphenyl diselenide on the levels of proteins involved in the hippocampal neuroprotection to figure out the mechanisms related to the beneficial effects of this intervention in aged rats...
May 25, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28539729/anti-fatigue-effect-of-green-tea-polyphenols-epigallocatechin-3-gallate-egcg
#8
Yu-Song Teng, Di Wu
BACKGROUND: (-)-Epigallocatechin-3-gallate (EGCG) is the most abundant of the green tea polyphenols that exhibit a variety of bioactivities. The objective of this study was to evaluate the anti-fatigue effect of EGCG by forced swimming exercise. MATERIALS AND METHODS: The mice were divided into one control group and three EGCG-treated groups. The control group was administered with distilled water and EGCG-treated groups were administered with different dose of EGCG (50, 100, and 200 mg/kg) by oral gavage for 28 days...
April 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28539728/anti-quorum-sensing-activity-of-forsythia-suspense-on-chromobacterium-violaceum-and-pseudomonas-aeruginosa
#9
An Zhang, Wei-Hua Chu
BACKGROUND: Quorum sensing (QS) plays an important role in the production of virulence factors and pathogenicity in Pseudomonas aeruginosa, and the interruption of QS will be a hopeful pathway to combat bacterial infection. OBJECTIVE: In this study, we selected Forsythia suspense (Thunb.) Vahl from traditional Chinese herbal medicines for its anti-QS activity. MATERIALS AND METHODS: Anti-QS of F. suspense extracts (FSE) was monitored using the Chromobacterium violaceum 12472 bioassay...
April 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28539713/content-determination-of-phenylpropanoids-and-enhancing-exercise-ability-of-effective-fractions-in-pedicularis-densispica
#10
Hongbiao Chu, Zhihua Zhang, Dong Chen, Xi Wang, Qilong Tu
BACKGROUND: Most researches were focused on chemical constituents and bioactivities of Pedicularis. However, there were a few reports on simultaneous determination of the series phenylpropanoids compounds in Pedicularis by High Performance Liquid Chromatography (HPLC). OBJECTIVE: To establish an HPLC method for simultaneous determination of salidroside, verbascoside, iso-verbascoside, leucoseptoside A, jionoside D and martynoside in Pedicularis densispica (PD), and to assess the enhancing exercise ability of effective fractions of phenylpropanoids (EFP)...
April 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28539670/simvastatin-and-bezafibrate-ameliorate-emotional-disorder-induced-by-high-fat-diet-in-c57bl-6-mice
#11
Hui Wang, Jia Zhou, Qiong Zhen Liu, Lu Lu Wang, Jing Shang
High fat diet (HFD)-induced metabolic disorders may lead to emotional disorders. This study aimed to explore the effect of simvastatin (SMV) and bezafibrate (BZ) on improving HFD-induced emotional changes, and tried to identify their different mechanisms. The intraperitoneal glucose tolerance test (IPGTT) was used to evaluate glucose control ability; and behavior tests including open field tests (OFT), forced swimming tests (FST), tail suspension tests (TST) and sucrose preference (SPT), were then performed to evaluate emotional changes...
May 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28539397/variations-on-a-theme-species-differences-in-synaptic-connectivity-do-not-predict-central-pattern-generator-activity
#12
Charuni A Gunaratne, Akira Sakurai, Paul S Katz
A fundamental question in comparative neuroethology is the extent to which synaptic wiring determines behavior versus the extent to which it is constrained by phylogeny. We investigated this by examining the connectivity and activity of homologous neurons in different species. Melibe leonina and Dendronotus iris (Mollusca, Gastropoda, Nudibranchia) have homologous neurons and exhibit homologous swimming behaviors consisting of alternating left-right (LR) whole body flexions. Yet, a homologous interneuron (Si1) differs between the two species in its participation in the swim motor pattern (SMP) and synaptic connectivity...
May 24, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28538315/evaluation-of-the-effectiveness-of-compression-garments-on-autonomic-nervous-system-recovery-after-exercise
#13
Alessandro Piras, Giorgio Gatta
Piras, A and Gatta, G. Evaluation of the effectiveness of compression garments on autonomic nervous system recovery after exercise. J Strength Cond Res 31(6): 1636-1643, 2017-The aim of this investigation was to evaluate the recovery pattern of a whole-body compression garment on hemodynamic parameters and on autonomic nervous system (ANS) activity after a swimming performance. Ten young male athletes were recruited and tested in 2 different days, with and without wearing the garment during the recovery phase...
June 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28538303/examining-fundamental-movement-competency-and-closed-chain-upper-extremity-dynamic-balance-in-swimmers
#14
Garrett S Bullock, Nate Brookreson, Amy M Knab, Robert J Butler
Bullock, GS, Brookreson, N, Knab, AM, and Butler, RJ. Examining fundamental movement competency and closed-chain upper-extremity dynamic balance in swimmers. J Strength Cond Res 31(6): 1544-1551, 2017-Abnormal fundamental movement patterns and upper-quarter dynamic balance are proposed mechanisms affecting athletic performance and injury risk. There are few studies investigating functional movement and closed-chain upper-extremity dynamic stability in swimmers. The purpose of this study was to determine differences in fundamental movement competency and closed-chain upper-extremity dynamic balance, using the Functional Movement Screen (FMS) and Upper-Quarter Y Balance Test (YBT-UQ), of high school (HS; n = 70) and collegiate (COL; n = 70) swimmers...
June 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28536913/histopathological-changes-in-zebrafish-embryos-exposed-to-dlpcbs-extract-from-zhanjiang-coastal-sediment
#15
Yunpeng Yu, Fanghong Nie, Anthony Hay, Hongying Lin, Yi Ma, Xianghong Ju, Dongliang Gong, Jinjun Chen, Ravi Gooneratne
Dioxin-like polychlorinated biphenyls (DLPCBs) are ubiquitous persistent pollutants that cause adverse effects in many environmental organisms. DLPCBs in marine sediments can be absorbed by benthic organisms, bioaccumulate, and biomagnify through the food chain and threaten animal and human health. There are no reports of DLPCBs concentrations in the Zhanjiang Gulf seabed. This study was designed to investigate the concentration of DLPCBs in the Zhanjiang coastal sediment and histopathological changes in zebrafish (Diano rerio) embryos exposed to environmentally relevant concentrations of DLPCBs...
June 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28536865/the-c-2-derivatives-of-salvinorin-a-ethoxymethyl-ether-sal-b-and-%C3%AE-tetrahydropyran-sal-b-have-anti-cocaine-properties-with-minimal-side-effects
#16
Amy W M Ewald, Peter J Bosch, Aimee Culverhouse, Rachel Saylor Crowley, Benjamin Neuenswander, Thomas E Prisinzano, Bronwyn M Kivell
RATIONALE: Kappa-opioid receptor (KOPr) agonists have pre-clinical anti-cocaine and analgesic effects. However, side effects including sedation, dysphoria, aversion, anxiety and depression limit their therapeutic development. The unique structure of salvinorin A has been used to develop longer acting KOPr agonists. OBJECTIVES: We evaluate two novel C-2 analogues of salvinorin A, ethoxymethyl ether Sal B (EOM Sal B) and β-tetrahydropyran Sal B (β-THP Sal B) alongside U50,488 for their ability to modulate cocaine-induced behaviours and side effects, pre-clinically...
May 23, 2017: Psychopharmacology
https://www.readbyqxmd.com/read/28536531/caffeine-and-bicarbonate-for-speed-a-meta-analysis-of-legal-supplements-potential-for-improving-intense-endurance-exercise-performance
#17
REVIEW
Peter M Christensen, Yusuke Shirai, Christian Ritz, Nikolai B Nordsborg
A 1% change in average speed is enough to affect medal rankings in intense Olympic endurance events lasting ~45 s to 8 min which for example includes 100 m swimming and 400 m running (~1 min), 1,500 m running and 4000 m track cycling (~4 min) and 2,000 m rowing (~6-8 min). To maximize the likelihood of winning, athletes utilizes legal supplements with or without scientifically documented beneficial effects on performance. Therefore, a continued systematic evidence based evaluation of the possible ergogenic effects is of high importance...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28536510/influence-of-pre-reproductive-maternal-enrichment-on-coping-response-to-stress-and-expression-of-c-fos-and-glucocorticoid-receptors-in-adolescent-offspring
#18
Debora Cutuli, Erica Berretta, Greta Pasqualini, Paola De Bartolo, Paola Caporali, Daniela Laricchiuta, Patricia Sampedro-Piquero, Francesca Gelfo, Matteo Pesoli, Francesca Foti, Azucena Begega, Laura Petrosini
Environmental enrichment (EE) is an experimental setting broadly used for investigating the effects of complex social, cognitive, and sensorimotor stimulations on brain structure and function. Recent studies point out that parental EE experience, even occurring in the pre-reproductive phase, affects neural development and behavioral trajectories of the offspring. In the present study we investigated the influences of pre-reproductive EE of female rats on maternal behavior and adolescent male offspring's coping response to an inescapable stressful situation after chronic social isolation...
2017: Frontiers in Behavioral Neuroscience
https://www.readbyqxmd.com/read/28535997/sperm-sexing-in-nili-ravi-buffalo-through-modified-swim-up-validation-using-sybr-%C3%A2-green-real-time-pcr
#19
Asma-Ul-Husna, Muhammad Amjad Awan, Abid Mehmood, Tasawar Sultana, Qaisar Shahzad, Muhammad Sajjad Ansari, Bushra Allah Rakha, S M Saqlan Naqvi, Shamim Akhter
Sperm sexing through flow-sorting technology is relatively expensive, requires considerable technical support and is actually not practicable in many developing countries. The aim of this study was to investigate the feasibility of producing enriched pools of X or Y chromosome-bearing sperm by a modified swim-up method. For this purpose semen was collected from five mature Nili-Ravi buffalo bulls for a period of six weeks. The qualifying ejaculates were divided into two aliquots for further processing through modified swim-up or control (untreated)...
May 6, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28535845/phylogenetic-distribution-of-the-euryarchaeal-archaellum-regulator-eara-and-complementation-of-a-methanococcus-maripaludis-%C3%A2-eara-mutant-with-heterologous-eara-homologues
#20
Yan Ding, Alison Berezuk, Cezar M Khursigara, Ken F Jarrell
Archaella are the swimming organelles in the Archaea. Recently, the first archaellum regulator in the Euryarchaeota, EarAMma, was identified in Methanococcus maripaludis, one of the model organisms used for archaellum studies. EarAMma binds to 6 bp consensus sequences upstream of the fla promoter to activate the transcription of the fla operon, which encodes most of the proteins required for archaella synthesis. In this study, synteny analysis showed that earA homologues are widely distributed in the phylum of Euryarchaeota, with the notable exception of extreme halophiles...
May 23, 2017: Microbiology
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