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https://www.readbyqxmd.com/read/28205414/avoidance-of-maternal-cell-contamination-and-overgrowth-in-isolating-fetal-chorionic-villi-mesenchymal-stem-cells-from-human-term-placenta
#1
Varda S Sardesai, Abbas Shafiee, Nicholas M Fisk, Rebecca A Pelekanos
Human placenta is rich in mesenchymal stem/stromal cells (MSC), with their origin widely presumed fetal. Cultured placental MSCs are confounded by a high frequency of maternal cell contamination. Our recent systematic review concluded that only a small minority of placental MSC publications report fetal/maternal origin, and failed to discern a specific methodology for isolation of fetal MSC from term villi. We determined isolation conditions to yield fetal and separately maternal MSC during ex vivo expansion from human term placenta...
February 16, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28202638/vitamin-concentrations-in-human-milk-vary-with-time-within-feed-circadian-rhythm-and-single-dose-supplementation
#2
Daniela Hampel, Setareh Shahab-Ferdows, M Munirul Islam, Janet M Peerson, Lindsay H Allen
Background: Human milk is the subject of many studies, but procedures for representative sample collection have not been established. Our improved methods for milk micronutrient analysis now enable systematic study of factors that affect its concentrations.Objective: We evaluated the effects of sample collection protocols, variations in circadian rhythms, subject variability, and acute maternal micronutrient supplementation on milk vitamin concentrations.Methods: In the BMQ (Breast-Milk-Quality) study, we recruited 18 healthy women (aged 18-26 y) in Dhaka, Bangladesh, at 2-4 mo of lactation for a 3-d supplementation study...
February 15, 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28199415/temperament-in-infancy-and-behavioral-and-emotional-problems-at-age-5-5-the-eden-mother-child-cohort
#3
Xian Abulizi, Laura Pryor, Grégory Michel, Maria Melchior, Judith van der Waerden
OBJECTIVE: Early temperamental characteristics may influence children's developmental pathways and predict future psychopathology. However, the environmental context may also shape or interact with infant temperament and indirectly contribute to increased vulnerability to adverse developmental outcomes. The aim of the present study is to explore the long-term contribution of temperamental traits at twelve months of age to the presence of emotional and behavioral problems later in childhood, and whether this association varies with the child's sex, parental separation, family socioeconomic status and maternal depression...
2017: PloS One
https://www.readbyqxmd.com/read/28197651/effects-of-early-life-stress-and-adolescent-ethanol-exposure-on-adult-cognitive-performance-in-the-5-choice-serial-reaction-time-task-in-wistar-male-rats
#4
Nathalie Boutros, Andre Der-Avakian, Athina Markou, Svetlana Semenova
RATIONALE: Early life stress combined with heavy adolescent alcohol use predicts impaired neuropsychological functioning in adulthood. We investigated whether adolescent intermittent ethanol (AIE) exposure combined with neonatal maternal separation in rats altered attentional processes and impulsivity in adulthood. METHODS: Male Wistar rat pups were exposed to maternal separation (postnatal days (PNDs) 1-14) and moderate AIE exposure (PNDs 28-57). Adult rats were tested in the five-choice serial reaction time task, which provides separate measures of attention, motor impulsivity, and compulsivity...
February 14, 2017: Psychopharmacology
https://www.readbyqxmd.com/read/28196405/psychosocial-interventions-for-supporting-women-to-stop-smoking-in-pregnancy
#5
REVIEW
Catherine Chamberlain, Alison O'Mara-Eves, Jessie Porter, Tim Coleman, Susan M Perlen, James Thomas, Joanne E McKenzie
BACKGROUND: Tobacco smoking remains one of the few preventable factors associated with complications in pregnancy, and has serious long-term implications for women and babies. Smoking in pregnancy is decreasing in high-income countries, but is strongly associated with poverty and is increasing in low- to middle-income countries. OBJECTIVES: To assess the effects of smoking cessation interventions during pregnancy on smoking behaviour and perinatal health outcomes...
February 14, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28193074/cobalamin-concentrations-in-fetal-liver-show-gender-differences-a-result-from-using-a-high-pressure-liquid-chromatography-inductively-coupled-plasma-mass-spectrometry-as-an-ultratrace-cobalt-speciation-method
#6
Janine Bosle, Sven Goetz, Andrea Raab, Eva M Krupp, Kirk G Scheckel, Enzo Lombi, Andrew A Meharg, Paul A Fowler, Jörg Feldmann
Maternal diet and lifestyle choices may affect placental transfer of cobalamin (Cbl) to the fetus. Fetal liver concentration of Cbl reflects nutritional status with regards to vitamin B12, but at these low concentration current Cbl measurement methods lack robustness. An analytical method based on enzymatic extraction with subsequent reversed-phase-high-pressure liquid chromatography (RP-HPLC) separation and parallel ICPMS and electrospray ionization (ESI)-Orbitrap-MS to determine specifically Cbl species in liver samples of only 10-50 mg was developed using 14 pig livers...
December 20, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/28188472/the-effect-of-postpartum-depression-and-current-mental-health-problems-of-the-mother-on-child-behaviour-at-eight-years
#7
R Closa-Monasterolo, M Gispert-Llaurado, J Canals, V Luque, M Zaragoza-Jordana, B Koletzko, V Grote, M Weber, D Gruszfeld, K Szott, E Verduci, A ReDionigi, J Hoyos, G Brasselle, J Escribano Subías
Background Maternal postpartum depression (PPD) could affect children's emotional development, increasing later risk of child psychological problems. The aim of our study was to assess the association between child's emotional and behavioural problems and mother's PPD, considering maternal current mental health problems (CMP). Methods This is a secondary analysis from the EU-Childhood Obesity Project (NCT00338689). Women completed the Edinburgh Postnatal Depression Scale (EPDS) at, 2, 3 and 6 months after delivery and the General Health Questionnaire (GHQ-12) to assess CMP once the children reached the age of 8 years...
February 11, 2017: Maternal and Child Health Journal
https://www.readbyqxmd.com/read/28185885/behavioral-effects-in-adolescence-and-early-adulthood-in-two-length-models-of-maternal-separation-in-male-rats
#8
María Banqueri, Marta Méndez, Jorge L Arias
Maternal separation (MS) is an extensively used early life stress model. There is some variability in the MS lengths used. Maternal separation leads to emotional and behavioral alterations such as anxiety, despair, or memory problems. We performed MS in Wistar rats with two length models from postnatal day 1 until day 10 and from postnatal day 1 until day 21 during 4h per day in both groups. We performed a test battery of a wide range of behaviors to measure anxiety, despair, prepulse inhibition, recognition memory, and associative memory both in adolescent and adult subjects...
February 7, 2017: Behavioural Brain Research
https://www.readbyqxmd.com/read/28183739/association-of-sociodemographic-and-household-characteristics-with-non-fatal-burns-among-children-under-the-age-of-10%C3%A2-years-in-sudan-an-exploratory-secondary-analysis-of-the-sudan-household-health-survey-2010
#9
Safa Abdalla, Muna Abdel Aziz, Igbal Basheir
BACKGROUND: Globally, children have the highest mortality rates from fire-related burns. Sudan is no exception, but there had been no prior investigations of potentially preventable risk factors. We undertook this analysis to investigate the role of various sociodemographic and household factors. METHODS: We used Sudan Household Health Survey 2010 data from a national stratified multistage cluster sample of 15 000 households. The dependent variable was whether the child had a non-fatal burn in the 12 months preceding the survey, based on the most recent injury...
February 9, 2017: Injury Prevention: Journal of the International Society for Child and Adolescent Injury Prevention
https://www.readbyqxmd.com/read/28179485/variation-in-sepsis-evaluation-across-a-national-network-of-nurseries
#10
Sagori Mukhopadhyay, James A Taylor, Isabelle Von Kohorn, Valerie Flaherman, Anthony E Burgos, Carrie A Phillipi, Nui Dhepyasuwan, Elizabeth King, Miren Dhudasia, Karen M Puopolo
BACKGROUND AND OBJECTIVES: The extent to which clinicians use currently available guidelines for early-onset sepsis (EOS) screening has not been described. The Better Outcomes through Research for Newborns network represents 97 nurseries in 34 states across the United States. The objective of this study was to describe EOS risk management strategies across a national sample of newborn nurseries. METHODS: A Web-based survey was sent to each Better Outcomes through Research for Newborns network nursery site representative...
February 8, 2017: Pediatrics
https://www.readbyqxmd.com/read/28179012/the-effect-of-mobile-application-interventions-on-influencing-healthy-maternal-behaviour-and-improving-perinatal-health-outcomes-a-systematic-review-protocol
#11
Lisa M Daly, Dell Horey, Philippa F Middleton, Frances M Boyle, Vicki Flenady
BACKGROUND: Perinatal morbidity and mortality remain significant public health issues globally, with enduring impact on the health and well-being of women and their families. Pregnant women who adopt, practice and maintain healthy behaviours can potentially improve the health of themselves and their babies. Mobile applications are an increasingly popular mode of accessing, storing and sharing health information among pregnant women. The main objective of this review is to evaluate the effects of mobile application interventions during pregnancy on maternal behaviour and associated maternal and infant outcomes...
February 8, 2017: Systematic Reviews
https://www.readbyqxmd.com/read/28178750/prolonged-maternal-separation-attenuates-bdnf-erk-signaling-correlated-with-spine-formation-in-the-hippocampus-during-early-brain-development
#12
Ken-Ichi Ohta, Shingo Suzuki, Katsuhiko Warita, Tomohiro Kaji, Takashi Kusaka, Takanori Miki
Maternal separation (MS) is known to affect hippocampal function such as learning and memory, yet the molecular mechanism remains unknown. We hypothesized that these impairments are attributed to abnormities of neural circuit formation by MS, and focused on brain-derived neurotrophic factor (BDNF) as key factor because BDNF signaling has an essential role in synapse formation during early brain development. Using rat offspring exposed to MS for 6 h/day during postnatal days (PD) 2-20, we estimated BDNF signaling in the hippocampus during brain development...
February 8, 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/28174020/necessity-is-the-mother-of-invention-ciliates-transposons-and-transgenerational-inheritance
#13
REVIEW
Sarah E Allen, Mariusz Nowacki
Ciliates are a fascinating model system for the study of the interaction between eukaryotic germlines and somatic lines, especially with regard to the invasion and defence against transposable elements. They separate their germline and somatic line into two nuclei within the same cell, and they silence transposons and repetitive elements by way of deleting them from their somatic genome. This large-scale deletion event uses a series of intricate sequence targeting pathways involving small RNAs and transposases, part of which consists of a transnuclear comparison between maternal soma and daughter germline...
February 4, 2017: Trends in Genetics: TIG
https://www.readbyqxmd.com/read/28170291/evaluating-a-medical-school-s-climate-for-women-s-success-outcomes-for-faculty-recruitment-retention-and-promotion
#14
Amparo C Villablanca, Yueju Li, Laurel A Beckett, Lydia Pleotis Howell
OBJECTIVE: Women are under-represented in academia. Causative factors include challenges of career-family integration. We evaluated factors reflecting institutional culture (promotion, retention, hiring, and biasing language in promotion letters) as part of an intervention to help shift culture and raise awareness of flexibility policies at the University of California, Davis (UCD). MATERIALS AND METHODS: Data on faculty use of family-friendly policies were obtained at baseline, and surveys for policy awareness were conducted pre(2010)/post(2013) an NIH-funded study educational intervention...
February 7, 2017: Journal of Women's Health
https://www.readbyqxmd.com/read/28169319/early-life-stress-impairs-recognition-memory-and-perturbs-the-functional-maturation-of-prefrontal-hippocampal-perirhinal-networks
#15
Samuel A J Reincke, Ileana L Hanganu-Opatz
Early life exposure to stressful situations impairs cognitive performance of adults and contributes to the etiology of several psychiatric disorders. Most of affected cognitive abilities rely on coupling by synchrony within complex neuronal networks, including prefrontal cortex (PFC), hippocampus (HP), and perirhinal cortex (PRH). Yet it remains poorly understood how early life stress (ELS) induces dysfunction within these networks during the course of development. Here we used intermittent maternal separation during the first 2 postnatal weeks to mimic ELS and monitored the recognition memory and functional coupling within prefrontal-hippocampal-perirhinal circuits in juvenile rats...
February 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28166792/mothers-pupillary-responses-to-infant-facial-expressions
#16
Santeri Yrttiaho, Dana Niehaus, Eileen Thomas, Jukka M Leppänen
BACKGROUND: Human parental care relies heavily on the ability to monitor and respond to a child's affective states. The current study examined pupil diameter as a potential physiological index of mothers' affective response to infant facial expressions. METHODS: Pupillary time-series were measured from 86 mothers of young infants in response to an array of photographic infant faces falling into four emotive categories based on valence (positive vs. negative) and arousal (mild vs...
February 6, 2017: Behavioral and Brain Functions: BBF
https://www.readbyqxmd.com/read/28164167/neonatal-maternal-separation-increases-susceptibility-to-experimental-colitis-and-acute-stress-exposure-in-male-mice
#17
Isabella M Fuentes, Natalie K Walker, Angela N Pierce, Briana R Holt, Elizabeth R Di Silvestro, Julie A Christianson
Experiencing early life stress can result in maladjusted stress response via dysregulation of the hypothalamic-pituitary-adrenal axis and serves as a risk factor for developing chronic pelvic pain disorders. We investigated whether neonatal maternal separation (NMS) would increase susceptibility to experimental colitis or exposure to acute or chronic stress. Male mice underwent NMS from postnatal day 1-21 and as adults were assessed for open field behavior, hindpaw sensitivity, and visceromotor response (VMR) to colorectal distension (CRD)...
December 2016: IBRO Rep
https://www.readbyqxmd.com/read/28162848/altered-functionality-of-the-corticotrophin-releasing-hormone-receptor-2-in-the-hypothalamic-paraventricular-nucleus-of-hyperphagic-maternally-separated-rats
#18
V Alcántara-Alonso, M I Amaya, G Matamoros-Trejo, P de Gortari
Early-life stress induces endocrine and metabolic alterations that increase food intake and overweight in adulthood. The stress response activates the corticotropin-releasing hormone (CRH) and urocortins' (Ucns) system in the hypothalamic paraventricular nucleus (PVN). These peptides induce anorexic effects through CRH-R2 receptor activation; however, chronic stressed animals develop hyperphagia despite of high PVN CRH expression. We analyzed this paradoxical behavior in adult rats subjected to maternal separation (MS) for 180min/daily during post-natal days 2-14, evaluating their body weight gain, food intake, serum corticosterone and vasopressin concentrations, PVN mRNA expression of CRH-R1, CRH-R2, CRH, Ucn2, Ucn3, vasopressin and CRH-R2 protein levels...
February 1, 2017: Neuropeptides
https://www.readbyqxmd.com/read/28161059/dnk-derived-ifn-%C3%AE-mediates-vsmc-migration-and-apoptosis-via-the-induction-of-lncrna-meg3-a-role-in-uterovascular-transformation
#19
Weifang Liu, Xiaoxia Liu, Minglian Luo, Xiaoping Liu, Qingqing Luo, Hui Tao, Di Wu, Sisi Lu, Jing Jin, Yin Zhao, Li Zou
INTRODUCTION: Appropriate spiral artery remodeling is critical for successful fetal development and pregnancy outcomes. The vascular smooth muscle cell (VSMC) loss and separation, involving cell apoptosis and migration, plays an important role in this process. Decidual natural killer cells (dNK)-derived interferon gamma (IFN-γ), a key regulator of uterine arterial remodeling, can facilitate separation of VSMC layers, however, the specific mechanisms of it action are unknown. Long non-coding RNA MEG3 functions as tumor suppressor by regulating apoptosis and migration...
February 2017: Placenta
https://www.readbyqxmd.com/read/28154300/effects-of-early-life-adverse-experiences-on-the-brain-implications-from-maternal-separation
#20
Mayumi Nishi, Takayo Sasagawa, Noriko Horii-Hayashi
No abstract text is available yet for this article.
2017: Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica
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