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ischemia and endoplasmic reticulum and autophagy

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https://www.readbyqxmd.com/read/27888631/autophagy-decreases-alveolar-macrophage-apoptosis-by-attenuating-endoplasmic-reticulum-stress-and-oxidative-stress
#1
Tao Fan, Lei Chen, Zhixin Huang, Zhangfan Mao, Wei Wang, Boyou Zhang, Yao Xu, Shize Pan, Hao Hu, Qing Geng
To study the impact of autophagy on alveolar macrophage apoptosis and its mechanism in the early stages of hypoxia, we established a cell hypoxia-reoxygenation model and orthotopic left lung ischemia-reperfusion model. Rat alveolar macrophages stably expressing RFP-LC3 were treated with autophagy inhibitor (3-methyladenine, 3-MA) or autophagy promoter (rapamycin), followed by hypoxia-reoxygenation treatment 2 h, 4 h or 6 h later. Twenty Sprague-Dawley male rats were randomly divided into four different groups: no blocking of left lung hilum (model group), left lung hilum blocked for 1h with DMSO lavage (control group), left lung hilum blocked for 1 h with 100 ml/kg 3-MA (5 μmol/L) lavage (3-MA group), and left lung hilum blocked for 1 h with 100 ml/kg rapamycin (250 nmol/L) lavage (rapamycin group)...
November 24, 2016: Oncotarget
https://www.readbyqxmd.com/read/27824068/sulforaphane-improves-ischemia-induced-detrusor-overactivity-by-downregulating-the-enhancement-of-associated-endoplasmic-reticulum-stress-autophagy-and-apoptosis-in-rat-bladder
#2
Huai-Ching Tai, Shiu-Dong Chung, Chiang-Ting Chien, Hong-Jeng Yu
Atherosclerosis-associated pelvic ischemia has been reported to be a risk factor for bladder dysfunction and subsequent lower urinary tract symptoms (LUTS) in the elderly population. However, the molecular mechanisms of this association remain unclear. We hypothesized that stress-induced cellular responses might play a role in the pathogenesis of ischemia-induced bladder dysfunction. In the present study, the animal model of bladder ischemia was induced by bilateral partial arterial occlusion (BPAO) in rats...
November 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27771898/trehalose-inhibits-protein-aggregation-caused-by-transient-ischemic-insults-through-preservation-of-proteasome-activity-not-via-induction-of-autophagy
#3
Ye Li, Yinan Luo, Tianfei Luo, Bin Lu, Chen Wang, Yanhong Zhang, Meihua Piao, Chunsheng Feng, Pengfei Ge
Protein aggregation has been proved to be a pathological basis accounting for neuronal death caused by either transient global ischemia or oxygen glucose deprivation (OGD), and inhibition of protein aggregation is emerging as a potential strategy of preventing brain damage. Trehalose was found to inhibit protein aggregation caused by neurodegenerative diseases via induction of autophagy, whereas its effect is still elusive on ischemia-induced protein aggregation. In this study, we investigated this issue by using rat model of transient global ischemia and SH-SY5Y model of OGD...
October 22, 2016: Molecular Neurobiology
https://www.readbyqxmd.com/read/27568838/associations-between-autophagy-the-ubiquitin-proteasome-system-and-endoplasmic-reticulum-stress-in-hypoxia-deoxygenation-or-ischemia-reperfusion
#4
Tao Fan, Zhixin Huang, Lei Chen, Wei Wang, Boyou Zhang, Yao Xu, Shize Pan, Zhangfan Mao, Hao Hu, Qing Geng
The activation of autophagy has been demonstrated to exert protective roles during hypoxia-reoxygenation (H/R)-induced brain injuries. This study aimed to investigate whether and how preconditioning with a proteasome inhibitor (MG-132), a proteasome promoter (Adriamycin, ADM), an autophagy inhibitor (3-methyladenine, 3-MA) and an autophagy promoter (Rapamycin, Rap) affected endoplasmic reticulum stress (ERS), the ubiquitin-proteasome system (UPS), autophagy, inflammation and apoptosis. Ubiquitin protein and 26S proteasome activity levels were decreased by MG-132 pretreatment but increased by ADM pretreatment at 2h, 4h and 6h following H/R treatment...
August 26, 2016: European Journal of Pharmacology
https://www.readbyqxmd.com/read/27317636/hydrogen-rich-saline-mediates-neuroprotection-through-the-regulation-of-endoplasmic-reticulum-stress-and-autophagy-under-hypoxia-ischemia-neonatal-brain-injury-in-mice
#5
Xuemei Bai, Song Liu, Lin Yuan, Yunkai Xie, Tong Li, Lingxiao Wang, Xueer Wang, Tiantian Zhang, Shucun Qin, Guohau Song, Li Ge, Zhen Wang
Hydrogen as a new medical gas exerts organ-protective effects through regulating oxidative stress, inflammation and apoptosis. Multiple lines of evidence reveal the protective effects of hydrogen in various models of brain injury. However, the exact mechanism underlying this protective effect of hydrogen against hypoxic-ischemic brain damage (HIBD) is not fully understood. The present study was designed to investigate whether hydrogen-rich saline (HS) attenuates HIBD in neonatal mice and whether the observed protection is associated with reduced endoplasmic reticulum (ER) stress and regulated autophagy...
September 1, 2016: Brain Research
https://www.readbyqxmd.com/read/26924060/autophagy-in-acute-kidney-injury
#6
REVIEW
Gur P Kaushal, Sudhir V Shah
Autophagy is a conserved multistep pathway that degrades and recycles damaged organelles and macromolecules to maintain intracellular homeostasis. The autophagy pathway is upregulated under stress conditions including cell starvation, hypoxia, nutrient and growth-factor deprivation, endoplasmic reticulum stress, and oxidant injury, most of which are involved in the pathogenesis of acute kidney injury (AKI). Recent studies demonstrate that basal autophagy in the kidney is vital for the normal homeostasis of the proximal tubules...
April 2016: Kidney International
https://www.readbyqxmd.com/read/26875637/orientin-protects-myocardial-cells-against-hypoxia-reoxygenation-injury-through-induction-of-autophagy
#7
Liya Liu, Youxi Wu, Xiulan Huang
Orientin, a flavonoid exists in Chinese traditional herbal Polygonum orientale L., has been previously demonstrated to protect against myocardial ischemia reperfusion injury (MIRI) through inhibition of apoptosis. However, the underlying mechanisms remain to be elucidated and we therefore in this study investigated the effects of orientin on autophagy during MIRI in rats. The results indicate that orientin, at the concentrations of 10 and 30 μM in the cultures of neonatal rat cardiomyocytes, promoted the induction of autophagy, increasing the formation of autophagosomes and enhancing the expression of LC3 puncta, LC3-II/LC3-I ratio and Beclin 1 after hypoxia/reoxygenation...
April 5, 2016: European Journal of Pharmacology
https://www.readbyqxmd.com/read/26797926/the-nuclear-melatonin-receptor-ror%C3%AE-is-a-novel-endogenous-defender-against-myocardial-ischemia-reperfusion-injury
#8
Ben He, Yichao Zhao, Longwei Xu, Lingchen Gao, Yuanyuan Su, Nan Lin, Jun Pu
Circadian rhythm disruption or decrease in levels of circadian hormones such as melatonin increases ischemic heart disease risk. The nuclear melatonin receptors RORs are pivotally involved in circadian rhythm regulation and melatonin effects mediation. However, the functional roles of RORs in the heart have never been investigated and were therefore the subject of this study on myocardial ischemia/reperfusion (MI/R) injury pathogenesis. RORα and RORγ subtypes were detected in the adult mouse heart, and RORα but not RORγ was downregulated after MI/R...
April 2016: Journal of Pineal Research
https://www.readbyqxmd.com/read/26711306/ire1-rack1-axis-orchestrates-er-stress-preconditioning-elicited-cytoprotection-from-ischemia-reperfusion-injury-in-liver
#9
Dong Liu, Xing Liu, Ti Zhou, William Yao, Jun Zhao, Zhigang Zheng, Wei Jiang, Fengsong Wang, Felix O Aikhionbare, Donald L Hill, Nerimah Emmett, Zhen Guo, Dongmei Wang, Xuebiao Yao, Yong Chen
Endoplasmic reticulum (ER) stress is involved in ischemic preconditioning that protects various organs from ischemia/reperfusion (I/R) injury. We established an in vivo ER stress preconditioning model in which tunicamycin was injected into rats before hepatic I/R. The hepatic I/R injury, demonstrated by serum aminotransferase level and the ultra-structure of the liver, was alleviated by administration of tunicamycin, which induced ER stress in rat liver by activating inositol-requiring enzyme 1 (IRE1) and upregulating 78 kDa glucose-regulated protein (GRP78)...
April 2016: Journal of Molecular Cell Biology
https://www.readbyqxmd.com/read/26683513/the-dichotomy-of-endoplasmic-reticulum-stress-response-in-liver-ischemia-reperfusion-injury
#10
COMPARATIVE STUDY
Haomming Zhou, Jianjun Zhu, Shi Yue, Ling Lu, Ronald W Busuttil, Jerzy W Kupiec-Weglinski, Xuehao Wang, Yuan Zhai
Endoplasmic reticulum (ER) stress plays critical roles in the pathogenesis of liver ischemia-reperfusion injury (IRI). As ER stress triggers an adaptive cellular response, the question of what determines its functional outcome in liver IRI remains to be defined. In a murine liver partial warm ischemia model, we studied how transient (30 minutes) or prolonged (90 minutes) liver ischemia regulated local ER stress response and autophagy activities and their relationship with liver IRI. Effects of chemical chaperon 4-phenylbutyrate (4-PBA) or autophagy inhibitor 3-methyladenine (3-MA) were evaluated...
February 2016: Transplantation
https://www.readbyqxmd.com/read/26562100/effects-of-placental-ischemia-are-attenuated-by-1-25-dihydroxyvitamin-d-treatment-and-associated-with-reduced-apoptosis-and-increased-autophagy
#11
Xiaoyu Tian, Suling Ma, Yaqi Wang, Lianguo Hou, Yun Shi, Min Yao, Xiaoning Wang, Huifeng Zhang, Lingling Jiang
We evaluated the effects of administration of 1,25-dihydroxyvitamin D (1,25(OH)2D) during pregnancy on relieving adverse outcomes of preeclampsia and the pathologic and biochemical changes in reduction in uteroplacental perfusion (RUPP) model of rats. On day 1, 7, and 14 of pregnancy, rats in pregnant RUPP plus 1,25(OH)2D (RUPP+VD) group (n = 15) received 120 ng/100 g body weight/week of 1,25(OH)2D by subcutaneous injection, while rats in normal pregnant (n = 12) and the RUPP group (n = 14) received 1,25(OH)2D vehicle (saline solution)...
February 2016: DNA and Cell Biology
https://www.readbyqxmd.com/read/26475925/endoplasmic-reticulum-stress-of-neutrophils-is-required-for-ischemia-reperfusion-induced-acute-lung-injury
#12
Rong Hu, Zhi-Feng Chen, Jia Yan, Qi-Fang Li, Yan Huang, Hui Xu, Xiao-Ping Zhang, Hong Jiang
Diverse clinical factors, including intestinal ischemia, contribute to acute lung injury (ALI), which has up to a 40% mortality rate. During the development of lung injury an immune response is elicited that exacerbates the lung insult. Neutrophils have been well studied in mediating the pulmonary insults through an assortment of mechanisms, such as release of granule contents and production of proinflammatory cytokines due to the overactivation of complement and cytokines. In this study, we found that enhanced endoplasmic reticulum (ER) stress was observed in infiltrated neutrophils in the early stage of an ALI mice model...
November 15, 2015: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/26473940/endoplasmic-reticulum-stress-and-ethanol-neurotoxicity
#13
REVIEW
Fanmuyi Yang, Jia Luo
Ethanol abuse affects virtually all organ systems and the central nervous system (CNS) is particularly vulnerable to excessive ethanol exposure. Ethanol exposure causes profound damages to both the adult and developing brain. Prenatal ethanol exposure induces fetal alcohol spectrum disorders (FASD) which is associated with mental retardation and other behavioral deficits. A number of potential mechanisms have been proposed for ethanol-induced brain damage; these include the promotion of neuroinflammation, interference with signaling by neurotrophic factors, induction of oxidative stress, modulation of retinoid acid signaling, and thiamine deficiency...
October 14, 2015: Biomolecules
https://www.readbyqxmd.com/read/26444017/endoplasmic-reticulum-stress-induced-autophagy-provides-cytoprotection-from-chemical-hypoxia-and-oxidant-injury-and-ameliorates-renal-ischemia-reperfusion-injury
#14
Bhavya B Chandrika, Cheng Yang, Yang Ou, Xiaoke Feng, Djamali Muhoza, Alexandrea F Holmes, Sue Theus, Sarika Deshmukh, Randy S Haun, Gur P Kaushal
We examined whether endoplasmic reticulum (ER) stress-induced autophagy provides cytoprotection from renal tubular epithelial cell injury due to oxidants and chemical hypoxia in vitro, as well as from ischemia-reperfusion (IR) injury in vivo. We demonstrate that the ER stress inducer tunicamycin triggers an unfolded protein response, upregulates ER chaperone Grp78, and activates the autophagy pathway in renal tubular epithelial cells in culture. Inhibition of ER stress-induced autophagy accelerated caspase-3 activation and cell death suggesting a pro-survival role of ER stress-induced autophagy...
2015: PloS One
https://www.readbyqxmd.com/read/26301516/micro-rna-30a-regulates-ischemia-induced-cell-death-by-targeting-heat-shock-protein-hspa5-in-primary-cultured-cortical-neurons-and-mouse-brain-after-stroke
#15
Peng Wang, Nan Zhang, Jia Liang, Jiefei Li, Song Han, Junfa Li
Micro-RNAs (miRs) have emerged as key gene regulators in many diseases, including stroke. We recently reported that miR-30a protects N2A cells against ischemic injury, in part through enhancing beclin 1-mediated autophagy. The present study explores further the involvement of miR-30a in ischemia-induced apoptosis and its possible mechanisms in primary cortical neurons and stroked mouse brain. We demonstrate that miR-30a level is significantly decreased in cortical neurons after 1-hr oxygen-glucose deprivation (OGD)/24-hr reoxygenation...
November 2015: Journal of Neuroscience Research
https://www.readbyqxmd.com/read/26293028/rapamycin-attenuates-mouse-liver-ischemia-and-reperfusion-injury-by-inhibiting-endoplasmic-reticulum-stress
#16
J Zhu, X Hua, D Li, J Zhang, Q Xia
The roles of endoplasmic reticulum (ER) stress in liver ischemia and reperfusion injury (IRI) have been well recognized. However, the impact of rapamycin (Rapa), a broadly used immunosuppressive agent in human liver transplantation, on ER stress during IRI remains unclear. This study was designed to investigate the roles of Rapa in the regulation of ER stress in vivo and in vitro. In a mouse liver partial warm ischemia and reperfusion mode, we demonstrated that Rapa markedly protected livers from IRI, as evidenced by serum alanine aminotransferase (sALT) levels and liver histology...
July 2015: Transplantation Proceedings
https://www.readbyqxmd.com/read/26289799/endoplasmic-reticulum-stress-in-brain-ischemia
#17
Yingchao Su, Feng Li
Endoplasmic reticulum (ER) stress is an intricate mechanism that mediates numerous responses during brain ischemia, thus being essential to determine the fate of neurons. In recent years, studies of the mechanisms of brain ischemic injury have centered on ER stress, glutamate excitotoxicity, dysfunction of mitochondria, inflammatory reactions, calcium overload and death receptor pathways. The role of ER stress is highly important. In addition to resulting in neuronal cell death through calcium toxicity and apoptotic pathways, ER stress also triggers a series of adaptive responses including unfolded protein response (UPR), autophagy, the expression of pro-survival proteins and the enhancement of ER self-repair ability, leading to less ischemic brain damage...
August 2016: International Journal of Neuroscience
https://www.readbyqxmd.com/read/26160707/ampk-energy-sensor-and-survival-mechanism-in-the-ischemic-heart
#18
REVIEW
Dake Qi, Lawrence H Young
AMP-activated protein kinase (AMPK) is a critical regulator of cellular metabolism and plays an important role in diabetes, cancer, and vascular disease. In the heart, AMPK activation is an essential component of the adaptive response to cardiomyocyte stress that occurs during myocardial ischemia. During ischemia-reperfusion, AMPK activation modulates glucose and fatty acid metabolism, mitochondrial function, endoplasmic reticulum (ER) stress, autophagy, and apoptosis. Pharmacological activation of AMPK prevents myocardial necrosis and contractile dysfunction during ischemia-reperfusion and potentially represents a cardioprotective strategy for the treatment of myocardial infarction...
August 2015: Trends in Endocrinology and Metabolism: TEM
https://www.readbyqxmd.com/read/26050121/endoplasmic-reticulum-stress-in-kidney-function-and-disease
#19
REVIEW
Mai Taniguchi, Hiderou Yoshida
PURPOSE OF REVIEW: Recently, a number of papers have reported that endoplasmic reticulum (ER) stress is involved in the onset of various kidney diseases, but the pathological mechanisms responsible have not been clarified. In this review, we summarize recent findings on this issue and try to clarify the pathology of ER stress-induced kidney diseases. RECENT FINDINGS: ER stress is evoked in various kidney diseases, including diabetic nephropathy, renal fibrosis, inflammation or osmolar contrast-induced renal injury, ischemia-reperfusion, genetic mutations of renal proteins, proteinuria and cyclosporine A treatment...
July 2015: Current Opinion in Nephrology and Hypertension
https://www.readbyqxmd.com/read/25832421/the-divergent-roles-of-autophagy-in-ischemia-and-preconditioning
#20
REVIEW
Rui Sheng, Zheng-hong Qin
Autophagy is an evolutionarily conserved and lysosome-dependent process for degrading and recycling cellular constituents. Autophagy is activated following an ischemic insult or preconditioning, but it may exert dual roles in cell death or survival during these two processes. Preconditioning or lethal ischemia may trigger autophagy via multiple signaling pathways involving endoplasmic reticulum (ER) stress, AMPK/TSC/mTOR, Beclin 1/BNIP3/SPK2, and FoxO/NF-κB transcription factors, etc. Autophagy then interacts with apoptotic and necrotic signaling pathways to regulate cell death...
April 2015: Acta Pharmacologica Sinica
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