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https://www.readbyqxmd.com/read/30220189/enhancement-of-expression-level-of-modified-t-pa-tnkase-in-leishmania-tarentolae-by-induction-system
#1
Mohammad Mehdi Attarpour Yazdi, Nikky Tofighi, Taraneh Rajaee, Marzieh Ghahremanlou, Ahmad Adeli, Bolhassani Azam, Mohammad Azizi, Noushin Davoudi
Background: The expression of bio-therapeutic proteins in mammalian cells, such as CHO, attains high homogeneity related to post-translational modifications. Although CHO remains the most popular cell line for bestselling biotherapeutic proteins on the market, there are still drawbacks such as expensive culture media, long time line, and high drug cost. Recently, researches on a novel Leishmania protozoan system have confirmed that this low level eukaryote could represent a competitive alternative to the mammalian cell lines...
September 17, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/30219864/smad7-in-keratinocytes-promotes-skin-carcinogenesis-by-activating-atm-dependent-dna-repair-and-an-egfr-mediated-cell-proliferation-pathway
#2
Huyen Trang Ha Thi, Hye-Youn Kim, Young-Jae Lee, Seong-Jin Kim, Suntaek Hong
SMAD7 is a general inhibitor of transforming growth factor β (TGF-β) signaling that acts through interaction and degradation of TGF-β receptors. SMAD7 has been demonstrated to be transcriptionally upregulated in chemical-induced skin tumors and TGF-β-treated normal keratinocytes. To evaluate the function of SMAD7 in skin carcinogenesis in vivo, Smad7 transgenic mice that specifically express either wild-type SMAD7 (TG-Smad7-WT) or mutant SMAD7 (TG-Smad7-MT) in keratinocytes, as well as Smad7 keratinocyte-specific knockout (Smad72f/2f-K14Cre) mice were subjected to chemical-induced skin carcinogenesis...
September 14, 2018: Carcinogenesis
https://www.readbyqxmd.com/read/30218285/retrovirus-mediated-transfection-of-the-tissue-type-plasminogen-activator-gene-results-in-increased-thrombolysis-of-blood-clots
#3
Xianhua Wu, Yongsheng Gong, Xuebing Ding, Gang Cheng, Weiya Yan, Xiaowei She, Changxing Wang, Xia Li
Tissue-type plasminogen activator (tPA) is involved in the lysis of blood clots. In this study, we attempted to target thrombolysis and enhance blood clot lysis by generating a construct (pLEGFP-N1-tPA) to integrate tPA gene into the genome of different cell lines. pLEGFP-N1-tPA construct was generated and used to target the tPA gene in different cell lines. The thrombolytic effects mediated by the supernatant from transfected HeLa cells and Linx cells were assessed using plasma thrombus plates. Furthermore, enhanced green fluorescent protein (EGFP), which was fused to the tPA gene in the pLEGFP-N1-tPA construct, was analyzed under the fluorescent microscope to assess tPA localization...
September 14, 2018: Biochemical Genetics
https://www.readbyqxmd.com/read/30217636/analysis-of-risk-factors-increased-hemorrhagic-transformation-after-acute-ischemic-stroke
#4
Wan-Qian Ge, Jie Chen, Hong Pan, Fei Chen, Cheng-Ye Zhou
BACKGROUND: The purpose of this study was to analyze the risk factors of hemorrhagic transformation (HT) after intravenous thrombolysis using a recombinant tissue plasminogen activator (r-tPA) in acute ischemic stroke (AIS). METHODS: We included 199 consecutive patients in the First Affiliated Hospital of Wenzhou Medical University from January 2016 to October 2017 with a diagnosis of AIS. The patients were divided into 2 groups: HT and non-HT. The related risk factors were recruited before and after receiving r-tPA thrombolysis...
September 11, 2018: Journal of Stroke and Cerebrovascular Diseases: the Official Journal of National Stroke Association
https://www.readbyqxmd.com/read/30216916/ketamine-ameliorates-depressive-like-behaviors-by-tpa-mediated-conversion-of-probdnf-to-mbdnf-in-the-hippocampus-of-stressed-rats
#5
Fan Zhang, Jie Luo, Xianlin Zhu
Some studies have indicated that ketamine has a rapid antidepressant effects, but the underlying molecular mechanism is still unclear. Researchers have found that mature brain-derived neurotrophic factor (mBDNF) and its precursor proBDNF are related to depression; they elicit opposite effects on cellular functions. It is clear that tissue plasminogen activator (tPA) is a key regulatory element in the conversion of proBDNF to mBDNF. The chronic unpredicted mild stress (CUMS) procedure is a classical and reliable method to establish the model of depression...
August 20, 2018: Psychiatry Research
https://www.readbyqxmd.com/read/30216061/combining-non-linear-optical-and-fluorescence-analysis-of-did-to-enhance-lipid-phase-recognition
#6
Silvio Osella, Florent Di Meo, N Arul Murugan, Gabin Fabre, Marcel Ameloot, Patrick Trouillas, Stefan Knippenberg
The widespread interest in phase recognition of lipid membranes has led to the use of different optical techniques to enable differentiation of healthy and not fully functional cells. In this work, we show how the combination of different (non-)linear optical methods such as One Photon Absorption (OPA), Two Photon Absorption (TPA) and Second Harmonic Generation (SHG) as well as the study of the fluorescence decay time leads to an enhanced screening of membrane phases using a fluorescent 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine (DiD) probe...
September 14, 2018: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/30199286/screen-time-policies-and-practices-in-early-care-and-education-centers-in-relationship-to-child-physical-activity
#7
Amanda E Staiano, Elizabeth Kipling Webster, Andrew T Allen, Amber R Jarrell, Corby K Martin
BACKGROUND: Few preschool children meet physical activity recommendations, whereas the majority exceeds screen-time recommendations. The purpose of this study was to examine the relationship of screen-time policies and practices with children's physical activity and sedentary time in early care and education (ECE) centers. METHODS: We conducted a cross-sectional study of 104 children (3.3 ± 0.5 years; 50% girls) attending 10 ECE centers in the United States...
August 2018: Childhood Obesity
https://www.readbyqxmd.com/read/30199217/facile-synthesis-of-terephthalic-acid-based-organic-fluorophore-with-strong-and-color-tunable-emission-in-both-solution-and-solid-states-for-led-applications
#8
Yuan Zhan, Lifeng Lin, Min Chen, Limin Wu
Dual-state emission (DSE) fluorophores with strong photoluminescence in both solution and solid states, have wide applications in fluorescent probes and photoelectric devices. However, most of the existing DSE fluorophores involve complex synthetic strategies or only one or two emission colors in solid state, which may hinder their practical applications. Herein, we report a facile and effective strategy to fabricate a kind of novel adjustable DSE fluorophore TPAA-Cu based on the small molecule terephthalic acid (TPA) and ascorbic acid (AA) with CuCl2 as the catalyst...
September 10, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/30197987/aptamer-based-proteomics-identifies-mortality-associated-serum-biomarkers-in-dialysis-dependent-aki-patients
#9
Li-Rong Yu, Jinchun Sun, Jaclyn R Daniels, Zhijun Cao, Laura Schnackenberg, Devasmita Choudhury, Paul M Palevsky, Jennie Z Ma, Richard D Beger, Didier Portilla
Introduction: Currently, no effective therapies exist to reduce the high mortality associated with dialysis-dependent acute kidney injury (AKI-D). Serum biomarkers may be useful in understanding the pathophysiological processes involved with AKI and the severity of injury, and point to novel therapeutic targets. Methods: Study day 1 serum samples from 100 patients and day 8 samples from 107 patients enrolled in the Veteran's Affairs/National Institutes of Health Acute Renal Failure Trial Network study were analyzed by the slow off-rate modified aptamers scan proteomic platform to profile 1305 proteins in each sample...
September 2018: KI Reports
https://www.readbyqxmd.com/read/30197114/impact-of-code-stroke-on-thrombolytic-therapy-in-patients-with-acute-ischemic-stroke-at-a-secondary-referral-hospital-in-taiwan
#10
Cheng-Lun Hsiao, Yu-Chin Su, Fu-Yi Yang, Chih-Yang Liu, Han-Lin Chiang, Guei-Chiuan Chen, Po-Jen Hsu, Pei-Ya Chen, Shinn-Kuang Lin
BACKGROUND: Efficacy of thrombolytic therapy decreases with time elapsed from symptom onset. We sought to identify the impact of code stroke on the thrombolytic therapy. METHODS: Code stroke is activated by the emergency physician when a patient is eligible for thrombolytic therapy. We retrospectively reviewed patients with acute ischemic stroke between January 2011 and December 2014. RESULTS: In total, 1809 patients were enrolled. Code stroke was activated in 233 of 351 patients arriving at the emergency room (ER) within 3 h of symptom onset, and in 21 patients arriving >3 h...
September 6, 2018: Journal of the Chinese Medical Association: JCMA
https://www.readbyqxmd.com/read/30194604/methods-for-assessing-serpins-as-neuroprotective-therapeutics
#11
Jacek M Kwiecien
As the systematic work on the pathogenesis of the white matter injury in the spinal cord models progresses, it becomes obvious that a severe and extraordinarily protracted, destructive inflammation follows the initial injury. Appropriate anti-inflammatory therapies of sufficient duration should not only inhibit but also lead to the elimination of this destructive inflammation, thus resulting in neuroprotection of the spinal cord tissue and a greater preservation of the neurologic function. While dexamethasone, a powerful, anti-inflammatory steroid analog administered continuously by subdural infusion for 7 days inhibited severe macrophage infiltration in the cavity of injury, the dose used was remarkably toxic...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/30194552/global-sagittal-alignment-in-elderly-patients-with-osteoporosis-and-its-relationship-with-severity-of-vertebral-fracture-and-quality-of-life
#12
Zongshan Hu, Gene C W Man, Anthony K L Kwok, Sheung-Wai Law, Winnie W C Chu, Wing-Hoi Cheung, Yong Qiu, Jack C Y Cheng
We compared global sagittal alignment and quality of life in osteoporotic patients with and without vertebral compression fracture (VCF) and determined its relationship with VCF severity. The findings revealed osteoporotic patients with VCF showed decreased quality of life and worse global sagittal alignment, which was significantly associated with VCF severity. INTRODUCTION: The aim of this study was to compare the global sagittal alignment and quality of life in elderly osteoporotic patients with and without vertebral compression fracture (VCF), and to investigate the relationship between global sagittal alignment and severity of VCF...
September 7, 2018: Archives of Osteoporosis
https://www.readbyqxmd.com/read/30192600/nonlinear-spectroscopy-with-x-ray-two-photon-absorption-in-metallic-copper
#13
Kenji Tamasaku, Eiji Shigemasa, Yuichi Inubushi, Ichiro Inoue, Taito Osaka, Tetsuo Katayama, Makina Yabashi, Akihiro Koide, Toshihiko Yokoyama, Tetsuya Ishikawa
X-ray two-photon absorption (TPA) spectrum of metallic copper is measured using a free-electron laser (XFEL). The spectrum differs from that measured by the conventional one-photon absorption (OPA), and characterized by a peak below the Fermi level, which is assigned to the transition to the 3d state. The impact of the XFEL pulse on the OPA spectrum is discussed by analyzing the pulse-energy dependence, which indicates that the intrinsic TPA spectrum is measured.
August 24, 2018: Physical Review Letters
https://www.readbyqxmd.com/read/30192506/tissue-plasminogen-activator-porous-magnetic-microrods-for-targeted-thrombolytic-therapy-after-ischemic-stroke
#14
Jiangnan Hu, Shengwei Huang, Lu Zhu, Weijie Huang, Yiping Zhao, Kunlin Jin, Qichuan Zhuge
Tissue plasminogen activator (tPA) is the only FDA approved thrombolytic drug for acute ischemic stroke but concerns regarding its limitations remain. Here, we developed a new strategy by incorporating tPA into porous magnetic iron oxide (Fe3 O4 )-microrods (tPA-MRs) for targeted thrombolytic therapy in ischemic stroke induced by distal middle cerebral artery occlusion. We showed that intra-arterial injection of tPA-MRs could target the cerebral blood clot in vivo under the guidance of an external magnet, where tPA was subsequently released at the site of embolism...
September 7, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/30191912/successful-intravenous-thrombolysis-of-a-wake-up-stroke-with-underlying-valvular-atrial-fibrillation
#15
K K Shahedah, C S Khoo, W Y Wan Nur Nafisah, C F Ng, I Noor Ashikin, M Y Mohd Naim, O Syazarina Sharis, Z Rozman, W Z Wan Asyraf
A 42-year-old female admitted with new-onset atrial fibrillation had a wake-up stroke on the high-dependency unit and the time last seen well (TLSW) was 6.5 h. She suffered left-sided body weakness and her National Institutes of Health Stroke Scale (NIHSS) score was 17. An emergency CT perfusion showed right M1 segment occlusion with more than 50% penumbra. She was given recombinant tissue plasminogen activator (r-tPA) at 9 h from TLSW. An immediate diagnostic angiogram with intention to treat, owing to the presence of large vessel occlusion, showed complete reperfusion after intravenous r-tPA...
September 2018: Journal of the Royal College of Physicians of Edinburgh
https://www.readbyqxmd.com/read/30186557/prognostic-factors-in-patients-with-acute-ischemic-stroke-treated-with-intravenous-tissue-plasminogen-activator-the-first-study-among-iranian-patients
#16
Elyar Sadeghi-Hokmabadi, Mohammad Yazdchi, Mehdi Farhoudi, Homayoun Sadeghi, Aliakbar Taheraghdam, Reza Rikhtegar, Hannaneh Aliyar, Sahar Mohammadi-Fallah, Rogayyeh Asadi, Elham Mehdizadeh-Far, Neda Ghaemian
Background: Tissue plasminogen activator (tPA) has been long approved as an efficacious treatment in patients with acute ischemic stroke (AIS); however, due to some serious complications, particularly intracranial hemorrhage (ICH), many physicians are still reluctant to use it liberally. This study sought to find potential prognostic factors in patients with AIS treated with tPA. Methods: A retrospective, hospital-bases observational study was conducted. Consecutively, a total of 132 patients with AIS treated with intravenous tPA, form June 2011 to July 2015 were enrolled...
January 5, 2018: Iranian Journal of Neurology
https://www.readbyqxmd.com/read/30186459/applications-and-development-of-permeability-imaging-in-ischemic-stroke
#17
REVIEW
Hui Chen, Guangming Zhu, Nan Liu, Ying Li, Yonghong Xia
Brain permeability imaging techniques are specific for the assessment of blood-brain barrier integrity. The present review article primarily focuses on the application of permeability imaging in cases of ischemic stroke. The permeability maps may be used to predict future hemorrhagic transformation in patients following acute ischemic stroke, that have been treated with tissue plasminogen activator (tPA) or recanalization therapy. The permeability imaging would help make the clinical decision to administer tPA following acute ischemic stroke or not, which is not only due to the current 3-4...
September 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30186221/optimal-delay-time-of-ct-perfusion-for-predicting-cerebral-parenchymal-hematoma-after-intra-arterial-tpa-treatment
#18
Bing Wu, Nan Liu, Max Wintermark, Mark W Parsons, Hui Chen, Longting Lin, Shuai Zhou, Gang Hu, Yongwei Zhang, Jun Hu, Ying Li, Zihua Su, Xinhuai Wu, Guangming Zhu
Background and Purpose: Cerebral hemorrhage is a serious potential complication of stroke revascularization, especially in patients receiving intra-arterial tissue-type plasminogen activator (tPA) therapy. We investigated the optimal pre-intervention delay time (DT) of computed tomography perfusion (CTP) measurement to predict cerebral parenchymal hematoma (PH) in acute ischemic stroke (AIS) patients after intra-arterial tissue plasminogen activator (tPA) treatment. Methods: The study population consisted of a series of patients with AIS who received intra-arterial tPA treatment and had CTP and follow-up computed tomography/magnetic resonance imaging (CT/MRI) to identify hemorrhagic transformation...
2018: Frontiers in Neurology
https://www.readbyqxmd.com/read/30180275/influence-of-methods-of-joint-inspection-during-tibial-plateau-leveling-osteotomy-on-the-radiographic-appearance-of-the-patellar-tendon
#19
Deandra J Owen, Rebecca Manley, Sue A Casale
OBJECTIVE: To determine the influence of methods of joint inspection during tibial plateau leveling osteotomy (TPLO) on radiographic appearance of the patellar tendon. STUDY DESIGN: Retrospective study. ANIMALS: Client-owned dogs (191) treated with TPLO (199). METHODS: Data collected from medical records included signalment, weight, duration of anesthesia and surgery, preoperative and postoperative tibial plateau angle (TPA), cranial cruciate ligament status, meniscal status, and meniscal treatment...
September 4, 2018: Veterinary Surgery: VS
https://www.readbyqxmd.com/read/30179096/effect-of-patellar-luxation-on-radiographic-measurements-of-tibial-plateau-angle-in-small-breed-dogs
#20
Michael J Orencole, Adam M Nylund, Jeremiah D Moorer, Robert W Wills, Tige H Witsberger
OBJECTIVE To determine whether medial patellar luxation would affect radiographic tibial plateau angle (TPA) measurements in small-breed dogs. DESIGN Prospective cross-sectional study. ANIMALS 15 small-breed dogs (25 stifle joints) with grade 2 or 3 medial patellar luxation (5 dogs with unilateral luxation and 10 dogs with bilateral luxation). PROCEDURES Digital mediolateral radiographic images of each affected stifle joint were acquired with the patella in manually reduced (n = 25) and luxated (25) positions...
September 15, 2018: Journal of the American Veterinary Medical Association
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