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Sex differences and proteomics

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https://www.readbyqxmd.com/read/28529466/the-serum-protein-responses-to-treatment-with-xiaoke-pill-and-glibenclamide-in-type-2-diabetes-patients
#1
Xiuying Zhang, Haidan Sun, Sanjoy K Paul, Quanhui Wang, Xiaomin Lou, Guixue Hou, Bo Wen, Linong Ji, Siqi Liu
AIM: The Xiaoke Pill containing Chinese herb extracts and Glibenclamide, is used in therapy for type 2 diabetes mellitus (T2DM), and is effective in reducing the risk of hypoglycemia and improving diabetes symptoms compared with Glibenclamide. We describe a quantitative proteomics project to measure the T2DM serum proteome response to the Xiaoke Pill and Glibenclamide. METHODS: Based on a recently conducted 48-week clinical trial comparing the safety and efficacy of Glibenclamide (n = 400) and Xiaoke Pill (n = 400), after matching for age, sex, BMI, drug dose and whether hypoglycemia occurred, 32 patients were selected for the serum based proteomic analysis and divided into four groups (with/without hypoglycemia treated with Xiaoke Pill or Glibenclamide, n = 8 for each group)...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28501205/an-integrated-strategy-for-the-quantitative-analysis-of-endogenous-proteins-a-case-of-gender-dependent-expression-of-p450-enzymes-in-rat-liver-microsome
#2
Yuhao Shao, Xiaoxi Yin, Dian Kang, Boyu Shen, Zhangpei Zhu, Xinuo Li, Haofeng Li, Lin Xie, Guangji Wang, Yan Liang
Liquid chromatography mass spectrometry based methods provide powerful tools for protein analysis. Cytochromes P450 (CYPs), the most important drug metabolic enzymes, always exhibit sex-dependent expression patterns and metabolic activities. To date, analysis of CYPs based on mass spectrometry is still facing critical technical challenges due to the complexity and diversity of CYP isoforms besides lack of corresponding standards. The aim of present work consisted in developing a label-free qualitative and quantitative strategy for endogenous proteins, and then applying to the gender-difference study for CYPs in rat liver microsomes (RLMs)...
August 1, 2017: Talanta
https://www.readbyqxmd.com/read/28468631/2d-dige-proteomic-analysis-of-vastus-lateralis-from-copd-patients-with-low-and-normal-fat-free-mass-index-and-healthy-controls
#3
Ramzi Lakhdar, Ellen M Drost, William MacNee, Ricardo Bastos, Roberto A Rabinovich
BACKGROUND: Chronic obstructive pulmonary disease (COPD) is associated with several extra-pulmonary effects of which skeletal muscle wasting is one of the most common and contributes to reduced quality of life, increased morbidity and mortality. The molecular mechanisms leading to muscle wasting are not fully understood. Proteomic analysis of human skeletal muscle is a useful approach for gaining insight into the molecular basis for normal and pathophysiological conditions. METHODS: To identify proteins involved in the process of muscle wasting in COPD, we searched differentially expressed proteins in the vastus lateralis of COPD patients with low fat free mass index (FFMI), as a surrogate of muscle mass (COPDL, n = 10) (FEV1 33 ± 4...
May 3, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28428745/decreased-levels-of-foldase-and-chaperone-proteins-are-associated-with-an-early-onset-amyotrophic-lateral-sclerosis
#4
Melania Filareti, Silvia Luotti, Laura Pasetto, Mauro Pignataro, Katia Paolella, Paolo Messina, Elisabetta Pupillo, Massimiliano Filosto, Christian Lunetta, Jessica Mandrioli, Giuseppe Fuda, Andrea Calvo, Adriano Chiò, Massimo Corbo, Caterina Bendotti, Ettore Beghi, Valentina Bonetto
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by a progressive upper and lower motor neuron degeneration. One of the peculiar clinical characteristics of ALS is the wide distribution in age of onset, which is probably caused by different combinations of intrinsic and exogenous factors. We investigated whether these modifying factors are converging into common pathogenic pathways leading either to an early or a late disease onset. This would imply the identification of phenotypic biomarkers, that can distinguish the two populations of ALS patients, and of relevant pathways to consider in a therapeutic intervention...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/28331360/clear-differences-in-cerebrospinal-fluid-proteome-between-women-with-chronic-widespread-pain-and-healthy-women-a-multivariate-explorative-cross-sectional-study
#5
Patrik Olausson, Bijar Ghafouri, Emmanuel Bäckryd, Björn Gerdle
INTRODUCTION: Frequent chronic local pain can develop into chronic widespread pain (CWP). The spread of pain is correlated with pain intensity, anxiety, and depression, conditions that ultimately lead to a poor quality of life. Knowledge is incomplete about CWP's etiology, although it has been suggested that both central hyperexcitability and/or a combination with peripheral factors may be involved. Cerebrospinal fluid (CSF) could act as a mirror for the central nervous system as proteins are signal substances that activate the formation of algesics and control nociceptive processes...
2017: Journal of Pain Research
https://www.readbyqxmd.com/read/28326097/different-proteome-profiles-between-male-and-female-populus-cathayana-exposed-to-uv-b-radiation
#6
Yunxiang Zhang, Lihua Feng, Hao Jiang, Yuanbin Zhang, Sheng Zhang
With increasing altitude, solar UV-B radiation is enhanced. Based on the phenomenon of male-biased sex ratio of Populus cathayana Rehder in high altitude alpine area, we hypothesized that males have a faster and more sophisticated responsive mechanism to high UV-B radiation than that of females. Our previous studies have shown sexually different responses to high UV-B radiation were existed in P. cathayana at the morphological, physiological, and transcriptomic levels. However, the responses at the proteomic level remain unclear...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28273097/characterization-of-the-human-aqueous-humour-proteome-a-comparison-of-the-genders
#7
Natarajan Perumal, Caroline Manicam, Matthias Steinicke, Sebastian Funke, Norbert Pfeiffer, Franz H Grus
Aqueous humour (AH) is an important biologic fluid that maintains normal intraocular pressure and contains proteins that regulate the homeostasis of ocular tissues. Any alterations in the protein compositions are correlated to the pathogenesis of various ocular disorders. In recent years, gender-based medicine has emerged as an important research focus considering the prevalence of certain diseases, which are higher in a particular sex. Nevertheless, the inter-gender variations in the AH proteome are unknown...
2017: PloS One
https://www.readbyqxmd.com/read/28243524/intersexual-differences-of-heat-shock-response-between-two-amphipods-eulimnogammarus-verrucosus-and-eulimnogammarus-cyaneus-in-lake-baikal
#8
Daria Bedulina, Michael F Meyer, Anton Gurkov, Ekaterina Kondratjeva, Boris Baduev, Roman Gusdorf, Maxim A Timofeyev
Acute temperature fluctuations are common in surface waters, and aquatic organisms may manifest physiological responses to punctuated temperature spikes long before behavioral responses. Ectotherms, especially cryophilic stenotherms such as those endemic to Lake Baikal (Siberia), may demonstrate specialized physiological responses to acute temperature increases because their proteomes have evolved to function most efficiently at lower temperatures (e.g., <10 °C). Therefore, our study questioned the nature and degree of variation in physiological response to acute thermal stress in two congenerous, endemic Baikal amphipod species, Eulimnogammarus verrucosus and Eulimnogammarus cyaneus...
2017: PeerJ
https://www.readbyqxmd.com/read/28240232/venomics-of-tropidolaemus-wagleri-the-sexually-dimorphic-temple-pit-viper-unveiling-a-deeply-conserved-atypical-toxin-arsenal
#9
Choo Hock Tan, Kae Yi Tan, Michelle Khai Khun Yap, Nget Hong Tan
Tropidolaemus wagleri (temple pit viper) is a medically important snake in Southeast Asia. It displays distinct sexual dimorphism and prey specificity, however its venomics and inter-sex venom variation have not been thoroughly investigated. Applying reverse-phase HPLC, we demonstrated that the venom profiles were not significantly affected by sex and geographical locality (Peninsular Malaya, insular Penang, insular Sumatra) of the snakes. Essentially, venoms of both sexes share comparable intravenous median lethal dose (LD50) (0...
February 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28159752/studies-of-an-androgen-binding-protein-knockout-corroborate-a-role-for-salivary-abp-in-mouse-communication
#10
Amanda G Chung, Phillip M Belone, Barbora Vošlajerová Bímová, Robert C Karn, Christina M Laukaitis
The house mouse Androgen-binding protein (Abp) gene family is comprised of 64 paralogs, 30 Abpa and 34 Abpbg, encoding the alpha (ABPA) and beta-gamma (ABPBG) protein subunits that are disulfide-bridged to form dimers in secretions. Only 14 Abp genes are expressed in distinct patterns in the lacrimal (11) and submandibular glands (3). We created a knockout mouse line lacking two of the three genes expressed in submandibular glands, Abpa27 and Abpbg27, by replacing them with the neomycin resistance gene. The knockout genotype (-/-) showed no Abpa27 or Abpbg27 transcripts in submandibular gland complementary DNA (cDNA) libraries and there was a concomitant lack of protein expression of ABPA27 and ABPBG27 in the -/- genotype saliva, shown by elimination of these two proteins from the saliva proteome and the loss of cross-reactive material in the acinar cells of the submandibular glands...
April 2017: Genetics
https://www.readbyqxmd.com/read/28126901/sex-specific-biology-of-the-human-malaria-parasite-revealed-from-the-proteomes-of-mature-male-and-female-gametocytes
#11
Jun Miao, Zhao Chen, Zenglei Wang, Sony Shrestha, Xiaolian Li, Runze Li, Liwang Cui
The gametocytes of the malaria parasites are obligate for perpetuating the parasite's life cycle through mosquitoes, but the sex-specific biology of gametocytes is poorly understood. We generated a transgenic line in the human malaria parasite Plasmodium falciparum, which allowed us to accurately separate male and female gametocytes by flow cytometry. In-depth analysis of the proteomes by liquid chromatography-tandem mass spectrometry identified 1244 and 1387 proteins in mature male and female gametocytes, respectively...
April 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28071813/sex-differences-in-tendon-structure-and-function
#12
Dylan C Sarver, Yalda Ashraf Kharaz, Kristoffer B Sugg, Jonathan P Gumucio, Eithne Comerford, Christopher L Mendias
Tendons play a critical role in the transmission of forces between muscles and bones, and chronic tendon injuries and diseases are among the leading causes of musculoskeletal disability. Little is known about sex-based differences in tendon structure and function. Our objective was to evaluate the mechanical properties, biochemical composition, transcriptome, and cellular activity of plantarflexor tendons from 4 month old male and female C57BL/6 mice using in vitro biomechanics, mass spectrometry-based proteomics, genome-wide expression profiling, and cell culture techniques...
January 10, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28062795/stable-isotope-labeling-with-amino-acids-silac-based-proteomics-of-primary-human-kidney-cells-reveals-a-novel-link-between-male-sex-hormones-and-impaired-energy-metabolism-in-diabetic-kidney-disease
#13
Sergi Clotet, Maria Jose Soler, Marta Riera, Julio Pascual, Fei Fang, Joyce Zhou, Ihor Batruch, Stella K Vasiliou, Apostolos Dimitromanolakis, Clara Barrios, Eleftherios P Diamandis, James W Scholey, Ana Konvalinka
Male sex predisposes to many kidney diseases. Considering that androgens exert deleterious effects in a variety of cell types within the kidney, we hypothesized that dihydrotestosterone (DHT) would alter the biology of the renal tubular cell by inducing changes in the proteome. We employed stable isotope labeling with amino acids (SILAC) in an indirect spike-in fashion to accurately quantify the proteome in DHT- and 17β-estradiol (EST)-treated human proximal tubular epithelial cells (PTEC). Of the 5043 quantified proteins, 76 were differentially regulated...
March 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28056817/proteomic-analyses-of-limbic-regions-in-neonatal-male-female-and-androgen-receptor-knockout-mice
#14
Anna Zettergren, Sara Karlsson, Erik Studer, Anna Sarvimäki, Petronella Kettunen, Annika Thorsell, Carina Sihlbom, Lars Westberg
BACKGROUND: It is well-established that organizational effects of sex steroids during early development are fundamental for sex-typical displays of, for example, mating and aggressive behaviors in rodents and other species. Male and female brains are known to differ with respect to neuronal morphology in particular regions of the brain, including the number and size of neurons, and the density and length of dendrites in nuclei of hypothalamus and amygdala. The aim of the present study was to use global proteomics to identify proteins differentially expressed in hypothalamus/amygdala during early development (postnatal day 8) of male, female and conditional androgen receptor knockout (AR(NesDel)) male mice, lacking androgen receptors specifically in the brain...
January 5, 2017: BMC Neuroscience
https://www.readbyqxmd.com/read/27998914/human-igm-antibodies-to-malondialdehyde-conjugated-with-albumin-are-negatively-associated-with-cardiovascular-disease-among-60-year-olds
#15
Divya Thiagarajan, Anna G Frostegård, Sudhir Singh, Mizanur Rahman, Anquan Liu, Max Vikström, Karin Leander, Bruna Gigante, Mai-Lis Hellenius, Bo Zhang, Roman A Zubarev, Ulf de Faire, Susanna L Lundström, Johan Frostegård
BACKGROUND: Malondialdehyde (MDA) is generated during lipid peroxidation as in oxidized low-density lipoprotein, but antibodies against oxidized low-density lipoprotein show variable results in clinical studies. We therefore studied the risk of cardiovascular disease (CVD) associated with IgM antibodies against MDA conjugated with human albumin (anti-MDA). METHODS AND RESULTS: In a 5- to 7-year follow-up of 60-year-old men and women from Stockholm County previously screened for cardiovascular risk factors (2039 men, 2193 women), 209 incident CVD cases (defined as new events of coronary heart disease, fatal and nonfatal myocardial infarction, ischemic stroke, and hospitalization for angina pectoris) and 620 age- and sex-matched controls were tested for IgM anti-MDA by ELISA...
December 20, 2016: Journal of the American Heart Association
https://www.readbyqxmd.com/read/27935948/alternative-splicing-within-and-between-drosophila-species-sexes-tissues-and-developmental-stages
#16
Lauren Gibilisco, Qi Zhou, Shivani Mahajan, Doris Bachtrog
Alternative pre-mRNA splicing ("AS") greatly expands proteome diversity, but little is known about the evolutionary landscape of AS in Drosophila and how it differs between embryonic and adult stages or males and females. Here we study the transcriptomes from several tissues and developmental stages in males and females from four species across the Drosophila genus. We find that 20-37% of multi-exon genes are alternatively spliced. While males generally express a larger number of genes, AS is more prevalent in females, suggesting that the sexes adopt different expression strategies for their specialized function...
December 2016: PLoS Genetics
https://www.readbyqxmd.com/read/27794105/use-of-a-proximity-extension-assay-proteomics-chip-to-discover-new-biomarkers-associated-with-albuminuria
#17
Axel C Carlsson, Johan Sundström, Juan Jesus Carrero, Stefan Gustafsson, Markus Stenemo, Anders Larsson, Lars Lind, Johan Ärnlöv
Background The underlying mechanisms for the development of albuminuria and the increased cardiovascular risk in patients with elevated albuminuria levels are incompletely understood. We therefore investigated the associations between 80 cardiovascular proteins and the urinary albumin to creatinine ratio (ACR). Methods We used a discovery/replication approach in two independent community-based cohorts of elderly patients: the Uppsala Longitudinal Study of Adult Men ( n = 662; mean age 78 years) and the Prospective Investigation of the Vasculature in Uppsala Seniors ( n = 757; mean age 75 years; 51% women)...
January 1, 2016: European Journal of Preventive Cardiology
https://www.readbyqxmd.com/read/27788204/changes-to-the-aqueous-humor-proteome-during-glaucoma
#18
Martha Andrea Kaeslin, Hanspeter Ezriel Killer, Cyril Adrian Fuhrer, Nauke Zeleny, Andreas Robert Huber, Albert Neutzner
PURPOSE: To investigate the aqueous humor proteome in patients with glaucoma and a control group. METHOD: Aqueous humor was obtained from five human donors diagnosed with primary open angle glaucoma (POAG) and five age- and sex-matched controls undergoing cataract surgery. Quantitative proteome analysis of the aqueous humor by hyper reaction monitoring mass spectrometry (HRM-MS) based on SWATH technology was performed. RESULTS: Expression levels of 87 proteins were found to be different between glaucomatous and control aqueous humor...
2016: PloS One
https://www.readbyqxmd.com/read/27761111/sexual-dimorphism-and-aging-in-the-human-hyppocampus-identification-validation-and-impact-of-differentially-expressed-genes-by-factorial-microarray-and-network-analysis
#19
Daniel V Guebel, Néstor V Torres
Motivation: In the brain of elderly-healthy individuals, the effects of sexual dimorphism and those due to normal aging appear overlapped. Discrimination of these two dimensions would powerfully contribute to a better understanding of the etiology of some neurodegenerative diseases, such as "sporadic" Alzheimer. Methods: Following a system biology approach, top-down and bottom-up strategies were combined. First, public transcriptome data corresponding to the transition from adulthood to the aging stage in normal, human hippocampus were analyzed through an optimized microarray post-processing (Q-GDEMAR method) together with a proper experimental design (full factorial analysis)...
2016: Frontiers in Aging Neuroscience
https://www.readbyqxmd.com/read/27745870/discovery-of-novel-plasma-proteins-as-biomarkers-for-the-development-of-incisional-hernias-after-midline-incision-in-patients-with-colorectal-cancer-the-colocare-study
#20
Jürgen Böhm, Frank Pianka, Nina Stüttgen, Junghyun Rho, Biljana Gigic, Yuzheng Zhang, Nina Habermann, Petra Schrotz-King, Clare Abbenhardt-Martin, Lin Zielske, Paul D Lampe, Alexis Ulrich, Markus K Diener, Cornelia M Ulrich
BACKGROUND: Ventral incisional hernia is the most common long-term complication after an abdominal operation. Among newly diagnosed colorectal cancer patients, we screened the preoperative plasma proteome to explore predictive markers for the development of an incisional hernia. METHODS: We utilized preoperative plasma samples of 72 newly diagnosed colorectal cancer patients who underwent midline incision for tumor resection between 2010 and 2013. A total of 21 patients with incisional hernia occurrence were matched with 51 patients with at least 18 months follow-up without an incisional hernia by sex, age, and body mass index...
March 2017: Surgery
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