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Sex differences and proteomics

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https://www.readbyqxmd.com/read/28914051/chromosome-centric-human-proteome-project-allies-with-developmental-biology-a-case-study-of-the-role-of-y-chromosome-genes-in-organ-development
#1
Anna Meyfour, Paria Pooyan, Sara Pahlavan, Mostafa Rezaei-Tavirani, Hamid Gourabi, Hossein Baharvand, Ghasem Hosseini Salekdeh
One of the main goals of Chromosome-Centric Human Proteome Project is to identify protein evidence for missing proteins (MPs). Here, we present a case study of the role of Y chromosome genes in organ development and how to overcome the challenges facing MPs identification by employing human pluripotent stem cell differentiation into cells of different organs yielding unprecedented biological insight into adult silenced proteins. Y chromosome is a male-specific sex chromosome which escapes meiotic recombination...
September 15, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28882797/modification-of-the-plasma-complement-protein-profile-by-exogenous-estrogens-is-indicative-of-a-compromised-immune-competence-in-marine-medaka-oryzias-melastigma
#2
Miao Dong, Frauke Seemann, Joseph L Humble, Yimin Liang, Drew R Peterson, Rui Ye, Honglin Ren, Hui-Su Kim, Jae-Seong Lee, Doris W T Au, Yun Wah Lam
Growing evidence suggests that the immune system of teleost is vulnerable to xenoestrogens, which are ubiquitous in the marine environment. This study detected and identified the major circulatory immune proteins deregulated by 17α-ethinylestradiol (EE2), which may be linked to fish susceptibility to pathogens in the marine medaka, Oryzias melastigma. Fish immune competence was determined using a host resistance assay to pathogenic bacteria Edwardsiella tarda. Females were consistently more susceptible to infection-induced mortality than males...
September 4, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28727099/identification-of-differentially-expressed-proteins-in-testicular-semen-of-sex-reversed-female-xx-and-normal-male-xy-rainbow-trout
#3
J Nynca, M Adamek, A Ciereszko
Masculinized females, named sex-reversed females (SRF), have a male phenotype but retain the female genotype (XX) and all spermatozoa produced in their testes carry the X chromosome. Masculinization of females leads to incomplete testicular development and the production of lower-quality semen. The mechanism of masculinization is unknown at present. Therefore, the aim of our study was to identify differentially abundant proteins in testicular semen of normal males and SRF using a difference in-gel electrophoresis approach...
July 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28698824/on-the-tear-proteome-of-the-house-mouse-mus-musculus-musculus-in-relation-to-chemical-signalling
#4
Romana Stopkova, Petr Klempt, Barbora Kuntova, Pavel Stopka
Mammalian tears are produced by lacrimal glands to protect eyes and may function in chemical communication and immunity. Recent studies on the house mouse chemical signalling revealed that major urinary proteins (MUPs) are not individually unique in Mus musculus musculus. This fact stimulated us to look for other sexually dimorphic proteins that may-in combination with MUPs-contribute to a pool of chemical signals in tears. MUPs and other lipocalins including odorant binding proteins (OBPs) have the capacity to selectively transport volatile organic compounds (VOCs) in their eight-stranded beta barrel, thus we have generated the tear proteome of the house mouse to detect a wider pool of proteins that may be involved in chemical signalling...
2017: PeerJ
https://www.readbyqxmd.com/read/28662025/biomolecular-changes-that-occur-in-the-antennal-gland-of-the-giant-freshwater-prawn-machrobrachium-rosenbergii
#5
Utpal Bose, Thanapong Kruangkum, Tianfang Wang, Min Zhao, Tomer Ventura, Shahida Akter Mitu, Mark P Hodson, Paul N Shaw, Prasert Sobhon, Scott F Cummins
In decapod crustaceans, the antennal gland (AnG) is a major primary source of externally secreted biomolecules, and some may act as pheromones that play a major role in aquatic animal communication. In aquatic crustaceans, sex pheromones regulate reproductive behaviours, yet they remain largely unidentified besides the N-acetylglucosamine-1,5-lactone (NAGL) that stimulates male to female attraction. In this study, we used an AnG transcriptome of the female giant freshwater prawn (Macrobrachium rosenbergii) to predict the secretion of 226 proteins, including the most abundantly expressed transcripts encoding the Spaetzle protein, a serine protease inhibitor, and an arthropodial cuticle protein AMP 8...
2017: PloS One
https://www.readbyqxmd.com/read/28562021/shotgun-proteomics-analysis-discards-alkali-labile-phosphate-as-a-reliable-method-to-assess-vitellogenin-levels-in-mytilus-galloprovincialis
#6
Paula Sánchez-Marín, Laura E Fernández-González, Leonardo Mantilla-Aldana, Angel P Diz, Ricardo Beiras
Vitellogenin, the egg yolk precursor, is a well-known biomarker of endocrine disruption in oviparous vertebrates. In invertebrates, such as bivalves, it has been used in the last 10 years for the same purpose, despite the limited knowledge of invertebrate endocrinology. In bivalves, vitellogenin levels are usually estimated using an indirect technique, alkali labile phosphate (ALP), that assumes that vitellogenin is the most abundant phosphorylated protein in the analyzed tissue. In this study, we applied shotgun proteomics for the identification and quantification of vitellogenin in marine mussel gonads and compared the results with those obtained with the ALP method...
June 13, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28529466/the-serum-protein-responses-to-treatment-with-xiaoke-pill-and-glibenclamide-in-type-2-diabetes-patients
#7
Xiuying Zhang, Haidan Sun, Sanjoy K Paul, Quanhui Wang, Xiaomin Lou, Guixue Hou, Bo Wen, Linong Ji, Siqi Liu
AIM: The Xiaoke Pill containing Chinese herb extracts and Glibenclamide, is used in therapy for type 2 diabetes mellitus (T2DM), and is effective in reducing the risk of hypoglycemia and improving diabetes symptoms compared with Glibenclamide. We describe a quantitative proteomics project to measure the T2DM serum proteome response to the Xiaoke Pill and Glibenclamide. METHODS: Based on a recently conducted 48-week clinical trial comparing the safety and efficacy of Glibenclamide (n = 400) and Xiaoke Pill (n = 400), after matching for age, sex, BMI, drug dose and whether hypoglycemia occurred, 32 patients were selected for the serum based proteomic analysis and divided into four groups (with/without hypoglycemia treated with Xiaoke Pill or Glibenclamide, n = 8 for each group)...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28501205/an-integrated-strategy-for-the-quantitative-analysis-of-endogenous-proteins-a-case-of-gender-dependent-expression-of-p450-enzymes-in-rat-liver-microsome
#8
Yuhao Shao, Xiaoxi Yin, Dian Kang, Boyu Shen, Zhangpei Zhu, Xinuo Li, Haofeng Li, Lin Xie, Guangji Wang, Yan Liang
Liquid chromatography mass spectrometry based methods provide powerful tools for protein analysis. Cytochromes P450 (CYPs), the most important drug metabolic enzymes, always exhibit sex-dependent expression patterns and metabolic activities. To date, analysis of CYPs based on mass spectrometry is still facing critical technical challenges due to the complexity and diversity of CYP isoforms besides lack of corresponding standards. The aim of present work consisted in developing a label-free qualitative and quantitative strategy for endogenous proteins, and then applying to the gender-difference study for CYPs in rat liver microsomes (RLMs)...
August 1, 2017: Talanta
https://www.readbyqxmd.com/read/28468631/2d-dige-proteomic-analysis-of-vastus-lateralis-from-copd-patients-with-low-and-normal-fat-free-mass-index-and-healthy-controls
#9
Ramzi Lakhdar, Ellen M Drost, William MacNee, Ricardo Bastos, Roberto A Rabinovich
BACKGROUND: Chronic obstructive pulmonary disease (COPD) is associated with several extra-pulmonary effects of which skeletal muscle wasting is one of the most common and contributes to reduced quality of life, increased morbidity and mortality. The molecular mechanisms leading to muscle wasting are not fully understood. Proteomic analysis of human skeletal muscle is a useful approach for gaining insight into the molecular basis for normal and pathophysiological conditions. METHODS: To identify proteins involved in the process of muscle wasting in COPD, we searched differentially expressed proteins in the vastus lateralis of COPD patients with low fat free mass index (FFMI), as a surrogate of muscle mass (COPDL, n = 10) (FEV1 33 ± 4...
May 3, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28428745/decreased-levels-of-foldase-and-chaperone-proteins-are-associated-with-an-early-onset-amyotrophic-lateral-sclerosis
#10
Melania Filareti, Silvia Luotti, Laura Pasetto, Mauro Pignataro, Katia Paolella, Paolo Messina, Elisabetta Pupillo, Massimiliano Filosto, Christian Lunetta, Jessica Mandrioli, Giuseppe Fuda, Andrea Calvo, Adriano Chiò, Massimo Corbo, Caterina Bendotti, Ettore Beghi, Valentina Bonetto
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by a progressive upper and lower motor neuron degeneration. One of the peculiar clinical characteristics of ALS is the wide distribution in age of onset, which is probably caused by different combinations of intrinsic and exogenous factors. We investigated whether these modifying factors are converging into common pathogenic pathways leading either to an early or a late disease onset. This would imply the identification of phenotypic biomarkers, that can distinguish the two populations of ALS patients, and of relevant pathways to consider in a therapeutic intervention...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/28331360/clear-differences-in-cerebrospinal-fluid-proteome-between-women-with-chronic-widespread-pain-and-healthy-women-a-multivariate-explorative-cross-sectional-study
#11
Patrik Olausson, Bijar Ghafouri, Emmanuel Bäckryd, Björn Gerdle
INTRODUCTION: Frequent chronic local pain can develop into chronic widespread pain (CWP). The spread of pain is correlated with pain intensity, anxiety, and depression, conditions that ultimately lead to a poor quality of life. Knowledge is incomplete about CWP's etiology, although it has been suggested that both central hyperexcitability and/or a combination with peripheral factors may be involved. Cerebrospinal fluid (CSF) could act as a mirror for the central nervous system as proteins are signal substances that activate the formation of algesics and control nociceptive processes...
2017: Journal of Pain Research
https://www.readbyqxmd.com/read/28326097/different-proteome-profiles-between-male-and-female-populus-cathayana-exposed-to-uv-b-radiation
#12
Yunxiang Zhang, Lihua Feng, Hao Jiang, Yuanbin Zhang, Sheng Zhang
With increasing altitude, solar UV-B radiation is enhanced. Based on the phenomenon of male-biased sex ratio of Populus cathayana Rehder in high altitude alpine area, we hypothesized that males have a faster and more sophisticated responsive mechanism to high UV-B radiation than that of females. Our previous studies have shown sexually different responses to high UV-B radiation were existed in P. cathayana at the morphological, physiological, and transcriptomic levels. However, the responses at the proteomic level remain unclear...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28273097/characterization-of-the-human-aqueous-humour-proteome-a-comparison-of-the-genders
#13
Natarajan Perumal, Caroline Manicam, Matthias Steinicke, Sebastian Funke, Norbert Pfeiffer, Franz H Grus
Aqueous humour (AH) is an important biologic fluid that maintains normal intraocular pressure and contains proteins that regulate the homeostasis of ocular tissues. Any alterations in the protein compositions are correlated to the pathogenesis of various ocular disorders. In recent years, gender-based medicine has emerged as an important research focus considering the prevalence of certain diseases, which are higher in a particular sex. Nevertheless, the inter-gender variations in the AH proteome are unknown...
2017: PloS One
https://www.readbyqxmd.com/read/28243524/intersexual-differences-of-heat-shock-response-between-two-amphipods-eulimnogammarus-verrucosus-and-eulimnogammarus-cyaneus-in-lake-baikal
#14
Daria Bedulina, Michael F Meyer, Anton Gurkov, Ekaterina Kondratjeva, Boris Baduev, Roman Gusdorf, Maxim A Timofeyev
Acute temperature fluctuations are common in surface waters, and aquatic organisms may manifest physiological responses to punctuated temperature spikes long before behavioral responses. Ectotherms, especially cryophilic stenotherms such as those endemic to Lake Baikal (Siberia), may demonstrate specialized physiological responses to acute temperature increases because their proteomes have evolved to function most efficiently at lower temperatures (e.g., <10 °C). Therefore, our study questioned the nature and degree of variation in physiological response to acute thermal stress in two congenerous, endemic Baikal amphipod species, Eulimnogammarus verrucosus and Eulimnogammarus cyaneus...
2017: PeerJ
https://www.readbyqxmd.com/read/28240232/venomics-of-tropidolaemus-wagleri-the-sexually-dimorphic-temple-pit-viper-unveiling-a-deeply-conserved-atypical-toxin-arsenal
#15
Choo Hock Tan, Kae Yi Tan, Michelle Khai Khun Yap, Nget Hong Tan
Tropidolaemus wagleri (temple pit viper) is a medically important snake in Southeast Asia. It displays distinct sexual dimorphism and prey specificity, however its venomics and inter-sex venom variation have not been thoroughly investigated. Applying reverse-phase HPLC, we demonstrated that the venom profiles were not significantly affected by sex and geographical locality (Peninsular Malaya, insular Penang, insular Sumatra) of the snakes. Essentially, venoms of both sexes share comparable intravenous median lethal dose (LD50) (0...
February 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28159752/studies-of-an-androgen-binding-protein-knockout-corroborate-a-role-for-salivary-abp-in-mouse-communication
#16
Amanda G Chung, Phillip M Belone, Barbora Vošlajerová Bímová, Robert C Karn, Christina M Laukaitis
The house mouse Androgen-binding protein (Abp) gene family is comprised of 64 paralogs, 30 Abpa and 34 Abpbg, encoding the alpha (ABPA) and beta-gamma (ABPBG) protein subunits that are disulfide-bridged to form dimers in secretions. Only 14 Abp genes are expressed in distinct patterns in the lacrimal (11) and submandibular glands (3). We created a knockout mouse line lacking two of the three genes expressed in submandibular glands, Abpa27 and Abpbg27, by replacing them with the neomycin resistance gene. The knockout genotype (-/-) showed no Abpa27 or Abpbg27 transcripts in submandibular gland complementary DNA (cDNA) libraries and there was a concomitant lack of protein expression of ABPA27 and ABPBG27 in the -/- genotype saliva, shown by elimination of these two proteins from the saliva proteome and the loss of cross-reactive material in the acinar cells of the submandibular glands...
April 2017: Genetics
https://www.readbyqxmd.com/read/28126901/sex-specific-biology-of-the-human-malaria-parasite-revealed-from-the-proteomes-of-mature-male-and-female-gametocytes
#17
COMPARATIVE STUDY
Jun Miao, Zhao Chen, Zenglei Wang, Sony Shrestha, Xiaolian Li, Runze Li, Liwang Cui
The gametocytes of the malaria parasites are obligate for perpetuating the parasite's life cycle through mosquitoes, but the sex-specific biology of gametocytes is poorly understood. We generated a transgenic line in the human malaria parasite Plasmodium falciparum, which allowed us to accurately separate male and female gametocytes by flow cytometry. In-depth analysis of the proteomes by liquid chromatography-tandem mass spectrometry identified 1244 and 1387 proteins in mature male and female gametocytes, respectively...
April 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28071813/sex-differences-in-tendon-structure-and-function
#18
Dylan C Sarver, Yalda Ashraf Kharaz, Kristoffer B Sugg, Jonathan P Gumucio, Eithne Comerford, Christopher L Mendias
Tendons play a critical role in the transmission of forces between muscles and bones, and chronic tendon injuries and diseases are among the leading causes of musculoskeletal disability. Little is known about sex-based differences in tendon structure and function. Our objective was to evaluate the mechanical properties, biochemical composition, transcriptome, and cellular activity of plantarflexor tendons from 4 month old male and female C57BL/6 mice using in vitro biomechanics, mass spectrometry-based proteomics, genome-wide expression profiling, and cell culture techniques...
January 10, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28062795/stable-isotope-labeling-with-amino-acids-silac-based-proteomics-of-primary-human-kidney-cells-reveals-a-novel-link-between-male-sex-hormones-and-impaired-energy-metabolism-in-diabetic-kidney-disease
#19
Sergi Clotet, Maria Jose Soler, Marta Riera, Julio Pascual, Fei Fang, Joyce Zhou, Ihor Batruch, Stella K Vasiliou, Apostolos Dimitromanolakis, Clara Barrios, Eleftherios P Diamandis, James W Scholey, Ana Konvalinka
Male sex predisposes to many kidney diseases. Considering that androgens exert deleterious effects in a variety of cell types within the kidney, we hypothesized that dihydrotestosterone (DHT) would alter the biology of the renal tubular cell by inducing changes in the proteome. We employed stable isotope labeling with amino acids (SILAC) in an indirect spike-in fashion to accurately quantify the proteome in DHT- and 17β-estradiol (EST)-treated human proximal tubular epithelial cells (PTEC). Of the 5043 quantified proteins, 76 were differentially regulated...
March 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28056817/proteomic-analyses-of-limbic-regions-in-neonatal-male-female-and-androgen-receptor-knockout-mice
#20
Anna Zettergren, Sara Karlsson, Erik Studer, Anna Sarvimäki, Petronella Kettunen, Annika Thorsell, Carina Sihlbom, Lars Westberg
BACKGROUND: It is well-established that organizational effects of sex steroids during early development are fundamental for sex-typical displays of, for example, mating and aggressive behaviors in rodents and other species. Male and female brains are known to differ with respect to neuronal morphology in particular regions of the brain, including the number and size of neurons, and the density and length of dendrites in nuclei of hypothalamus and amygdala. The aim of the present study was to use global proteomics to identify proteins differentially expressed in hypothalamus/amygdala during early development (postnatal day 8) of male, female and conditional androgen receptor knockout (AR(NesDel)) male mice, lacking androgen receptors specifically in the brain...
January 5, 2017: BMC Neuroscience
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