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Sex differences and proteomics

Yu Shi, Wenguang Liu, Maoxian He
Bivalve mollusks exhibit hermaphroditism and sex reversal/differentiation. Studies generally focus on transcriptional profiling and specific genes related to sex determination and differentiation. Few studies on sex reversal/differentiation have been reported. A combination analysis of gonad proteomics and transcriptomics was conducted on Chlamys nobilis to provide a systematic understanding of sex reversal/differentiation in bivalves. We obtained 4258 unique peptides and 93,731 unigenes with good correlation between messenger RNA and protein levels...
March 15, 2018: Marine Biotechnology
Sineepat Talungchit, Waranun Buajeeb, Chotima Lerdtripop, Rudee Surarit, Kongthawat Chairatvit, Sittiruk Roytrakul, Hiroaki Kobayashi, Yuichi Izumi, Siribang-On Piboonniyom Khovidhunkit
BACKGROUND: Salivary protein biomarkers for screening and diagnosis of oral lichen planus (OLP) are not well-defined. The objective of this study was to identify putative protein biomarkers for OLP using proteomic approaches. METHODS: Pooled unstimulated whole saliva was collected from five OLP patients and five healthy control participants. Saliva samples were then subjected to two-dimensional gel electrophoresis, followed by mass spectrometry to identify putative protein biomarkers...
March 13, 2018: BMC Oral Health
Maree J Webster, Sanghyeon Kim
The Stanley Medical Research Institute Brain Collection distributes samples from specified cohorts that contain demographically matched groups of subjects with mental illnesses such as schizophrenia, bipolar disorder, and major depression, as well as unaffected controls. The groups are matched by age, sex, race, postmortem interval, pH, side of brain, and mRNA quality. The samples are distributed coded so that all data must be returned in order to obtain the demographic information. The database contains more than 5000 individual data sets, as well as data from high-throughput microarray, sequencing, and proteomic studies...
2018: Handbook of Clinical Neurology
Siamack Sabrkhany, Marijke J E Kuijpers, Jaco C Knol, Steven W M Olde Damink, Anne-Marie C Dingemans, Henk M Verheul, Sander R Piersma, Thang V Pham, Arjan W Griffioen, Mirjam G A Oude Egbrink, Connie R Jimenez
Platelets play an important role in tumor growth and, at the same time, platelet characteristics are affected by cancer presence. Therefore, we investigated whether the platelet proteome harbors differentially expressed proteins associated with early-stage cancer. For this proof-of-concept study, patients with early-stage lung (n = 8) or head of pancreas cancer (n = 4) were included, as were healthy sex- and age-matched controls for both subgroups. Blood samples were collected from controls and from patients before surgery...
February 9, 2018: Journal of Proteomics
Zhi Chen, Jie Li, Siwen Gui, Chanjuan Zhou, Jianjun Chen, Chuangchuang Yang, Zicheng Hu, Haiyang Wang, Xiaogang Zhong, Li Zeng, Ke Chen, Pengfei Li, Peng Xie
Major depressive disorder (MDD) is a highly prevalent and debilitating mental illness, which is associated with disorder of gut microbiota. However, few studies focusing on detection of the signatures of bacteria in feces of MDD patients using proteomics approach have been carried out. Here, a comparative metaproteomics analysis on the basis of an isobaric tag for relative and absolute quantification coupled with tandem mass spectrometry was carried out to explore the signature of gut microbiota in patients with MDD...
February 9, 2018: Neuroreport
Zi-Xuan Zhuang, Shen-Chang Chang, Chao-Jung Chen, Hong-Lin Chan, Min-Jung Lin, Hsin-Yi Liao, Chuen-Yu Cheng, Tsung-Yi Lin, Yu-Shine Jea, San-Yuan Huang
The purpose of this study was to investigate the change in protein expression in the testes of ganders at various breeding stages. A total of nine 3-year-old male White Roman ganders were used. The blood and testis samples were collected at the nonbreeding, sexual reactivation, and breeding stages for sex hormone analysis and proteomic analysis, respectively. The testicular weight and serum testosterone observed for ganders at the breeding stage were higher than those for ganders at nonbreeding and sexual reactivation stages (P < 0...
February 9, 2018: Animal Biotechnology
Angela Baralla, Alessandro G Fois, Elisabetta Sotgiu, Elisabetta Zinellu, Arduino A Mangoni, Salvatore Sotgia, Angelo Zinellu, Pietro Pirina, Ciriaco Carru
PURPOSE: Chronic obstructive pulmonary disease (COPD) is characterized by progressive airflow limitation and abnormal inflammatory response of the lungs to inhaled noxious particles or gases. We used a proteomic approach with 2-DE followed by MALDI TOF-MS analyses in order to identify potential biomarkers in the early stages of the disease: GOLD stage mild and moderate. EXPERIMENTAL DESIGN: Blood plasma was collected from 43 patients with mild and moderate COPD as well as from 43 age and sex matched control subjects...
February 7, 2018: Proteomics. Clinical Applications
Daniel Boda, Anca Oana Docea, Daniela Calina, Mihaela Adriana Ilie, Constantin Caruntu, Sabina Zurac, Monica Neagu, Carolina Constantin, Daciana Elena Branisteanu, Vlad Voiculescu, Charalampos Mamoulakis, George Tzanakakis, Demetrios A Spandidos, Nikolaos Drakoulis, Aristides M Tsatsakis
Human papilloma viruses (HPV) are a small group of non‑enveloped viruses belonging to the Papillomaviridae family with strong similarities to polyoma viruses. The viral particles consist of a genome in the form of a circular double‑stranded DNA, encompassing eight open reading frames, as well as a non‑enveloped icosahedral capsid. HPV infection is considered the most common sexually transmitted disease in both sexes and is strongly implicated in the pathogenesis of different types of cancer. 'High‑risk' mucosal HPV types, predominantly types 16, 18, 31, 33 and 35, are associated with most cervical, penile, vulvar, vaginal, anal, oropharyngeal cancers and pre‑cancers...
January 29, 2018: International Journal of Oncology
Janejira Kittivorapart, Vanja Karamatic Crew, Marieangela C Wilson, Kate J Heesom, Noppadol Siritanaratkul, Ashley M Toye
Hemoglobin E (HbE)/β-thalassemia has a wide spectrum of clinical manifestations that cannot be explained purely by its genetic background. Circulating extracellular vesicles (EVs) are one factor that likely contributes to disease severity. This study has explored the differences in protein composition and quantity between EVs from HbE/β-thalassemic patients and healthy individuals. We used tandem mass tag labeling mass spectrometry to analyze the EV proteins isolated from the plasma of 15 patients compared with the controls...
January 23, 2018: Blood Advances
Chui-Wen Deng, Li Wang, Yun-Yun Fei, Chao-Jun Hu, Yun-Jiao Yang, Lin-Yi Peng, Xiao-Feng Zeng, Feng-Chun Zhang, Yong-Zhe Li
AIM: To explore the pathogenesis of primary biliary cholangitis (PBC) by identifying candidate autoantibodies in serum samples by proteomics and bioinformatics. METHODS: Nine antimitochondrial antibody (AMA)-positive PBC patients and nine age- and sex-matched AMA-negative PBC patients were recruited. Antigen enrichment technology was applied to capture autoantigens of human intrahepatic biliary epithelial cells (HiBECs) that are recognized by autoantibodies from the sera of PBC patients...
December 28, 2017: World Journal of Gastroenterology: WJG
L Soler, I P Oswald
Mycotoxins are natural food and feed contaminants that are toxic to human and animals. Proteomics is an adequate toolbox to investigate the mode of action and the effects of mycotoxins, as these toxicants often alter protein synthesis and degradation, as well as induce changes of important post-translational modifications. For instance, the contaminant deoxynivalenol induces a severe ribosomal stress that affects protein production, whereas the toxin Fumonisin B1 can alter the phosphorylation of a large number of proteins, and patulin is a potent proteotoxic molecule...
December 26, 2017: Journal of Proteomics
Xi-Ming Yuan, Liam J Ward, Claes Forssell, Nabeel Siraj, Wei Li
BACKGROUND AND PURPOSE: Men differ from women in the manifestation of atherosclerosis and iron metabolism. Intraplaque hemorrhage and hemoglobin (Hb) catabolism by macrophages are associated with atherosclerotic lesion instability. The study aims were to investigate sex differences in (1) lesion severity in relation to blood Hb, (2) iron homeostasis in human carotid plaques, and (3) macrophage polarization within atheroma. METHODS: The carotid artery samples from 39 men and 23 women were immunostained with cell markers for macrophages, smooth muscle cells, ferritin, and TfR1 (transferrin receptor 1), which were further analyzed according to sex in relation to iron, Hb, and lipids in circulation...
December 28, 2017: Stroke; a Journal of Cerebral Circulation
Franz Theuring, Boris Neumann, Christian Scheler, Peter R Jungblut, Karima Schwab
We investigated sex differences in cardiac protein patterns of intact and castrated mice using proteomics and 1D and 2D immunoblotting. To exclude differences concerning developmental aspects gonadectomy was conducted in mature mice at the age of three months. The main sex-related regulation in the protein pattern of the myocardium occurred for proteins involved in metabolic processes whereas only few proteins involved in other pathways underwent a regulation. Many regulated proteins (2/3) displayed a characteristic V form, which means that these proteins are up- or down-regulated in sexually mature compared to young mice and are back-regulated after castration, emphasizing a direct regulation by gonadal hormones...
December 19, 2017: Journal of Proteomics
Grace Xiong, Nithya Lingampalli, Jayme C B Koltsov, Lawrence L Leung, Nidhi Bhutani, William H Robinson, Constance R Chu
BACKGROUND: Autologous platelet-rich plasma (PRP) is widely used for a variety of clinical applications. However, clinical outcome studies have not consistently shown positive effects. The composition of PRP differs based on many factors. An improved understanding of factors influencing the composition of PRP is important for the optimization of PRP use. HYPOTHESIS: Age and sex influence the PRP composition in healthy patients. STUDY DESIGN: Controlled laboratory study...
February 2018: American Journal of Sports Medicine
George T Noutsios, Nithyananda Thorenoor, Xuesheng Zhang, David S Phelps, Todd M Umstead, Faryal Durrani, Joanna Floros
BACKGROUND: Human innate host defense molecules, surfactant protein A1 (SP-A1), and SP-A2 differentially affect the function and proteome of the alveolar macrophage (AM). We hypothesized that SP-A genes differentially regulate the AM miRNome. METHODS: Humanized transgenic mice expressing SP-A1 and SP-A2 were subjected to O3 -induced oxidative stress (OxS) or filtered air (FA), AMs were isolated, and miRNA levels were measured. RESULTS: In SP-A2 males, we found significant changes in miRNome in terms of sex and sex-OxS effects, with 11 miRNAs differentially expressed under OxS...
December 4, 2017: Biology of Sex Differences
Roberta Baetta, Marta Pontremoli, Alma Martinez Fernandez, Corinne M Spickett, Cristina Banfi
Cardiovascular diseases (CVDs) represent the most important cause of mortality in women and in men. Contrary to the long-standing notion that the effects of the major risk factors on CVD outcomes are the same in both sexes, recent evidence recognizes new, potentially independent, sex/gender-related risk factors for CVDs, and sex/gender-differences in the clinical presentation of CVDs have been demonstrated. Furthermore, some therapeutic options may not be equally effective and safe in men and women. In this context, proteomics offers an extremely useful and versatile analytical platform for biomedical researches that expand from the screening of early diagnostic and prognostic biomarkers to the investigation of the molecular mechanisms underlying CDVs...
February 20, 2018: Journal of Proteomics
Jitong Sun, Susanna L Lundström, Bo Zhang, Roman A Zubarev, Johnny Steuer, Peter Gillgren, Mizanur Rahman, Sofia Ajeganova, Anquan Liu, Johan Frostegård
BACKGROUND AND AIMS: IgM antibodies against phosphorylcholine (anti-PC) are negatively associated with atherosclerosis, cardiovascular disease (CVD) and systemic lupus erythematosus (SLE), where the risk of CVD and atherosclerosis is high. We here study the effects of IgM anti-PC immune regulation. METHODS: Mononuclear leukocytes were isolated from peripheral blood (PBMC) obtained from healthy blood donors, six SLE patients with age- and sex-matched controls, and symptom-giving human atherosclerotic plaques...
January 2018: Atherosclerosis
Haifeng Song, Yanbao Lei, Sheng Zhang
In most forest soils, the availability of nitrogen (N) and phosphorus (P) nutrients is low and unable to meet the requirement of tree growth. In the past decades, sex-based differences in poplar have been investigated in morphology and physiology. Proteomic techniques provide new insights into sex-specific differences at the molecular level. This review gives a comparative overview of the effects of N and P deficiencies on poplar physiological and proteomic characteristics. Male poplars are more efficient at photosynthesis and nutrient usage than females...
November 21, 2017: Journal of Proteomics
Olivier D'Amours, Gilles Frenette, Sylvie Bourassa, Ézéchiel Calvo, Patrick Blondin, Robert Sullivan
Mammalian semen contains a heterogeneous population of sperm cells. This heterogeneity results from variability in the complex processes of cell differentiation in the testis, biochemical modifications undergone by spermatozoa during transit along the male reproductive tract, interactions with secretions from accessory sex glands at ejaculation, and, in the context of reproductive technologies, in the ability of ejaculated spermatozoa to resist damage associated with freeze-thaw procedures. When submitted to density gradient centrifugation, ejaculated spermatozoa distribute themselves into two distinct populations: a low-density population characterized by low motility parameters, and a high-density population with high motility characteristics...
January 5, 2018: Journal of Proteome Research
Claudia Pontillo, Zhen-Yu Zhang, Joost P Schanstra, Lotte Jacobs, Petra Zürbig, Lutgarde Thijs, Adela Ramírez-Torres, Hiddo J L Heerspink, Morten Lindhardt, Ronald Klein, Trevor Orchard, Massimo Porta, Rudolf W Bilous, Nishi Charturvedi, Peter Rossing, Antonia Vlahou, Eva Schepers, Griet Glorieux, William Mullen, Christian Delles, Peter Verhamme, Raymond Vanholder, Jan A Staessen, Harald Mischak, Joachim Jankowski
Introduction: CKD273 is a urinary biomarker, which in advanced chronic kidney disease predicts further deterioration. We investigated whether CKD273 can also predict a decline of estimated glomerular filtration rate (eGFR) to <60 ml/min per 1.73 m2. Methods: In analyses of 2087 individuals from 6 cohorts (46.4% women; 73.5% with diabetes; mean age, 46.1 years; eGFR ≥ 60 ml/min per 1.73 m2, 100%; urinary albumin excretion rate [UAE] ≥20 μg/min, 6.2%), we accounted for cohort, sex, age, mean arterial pressure, diabetes, and eGFR at baseline and expressed associations per 1-SD increment in urinary biomarkers...
November 2017: KI Reports
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