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Sex differences and proteomics

Daniel V Guebel, Néstor V Torres
Motivation: In the brain of elderly-healthy individuals, the effects of sexual dimorphism and those due to normal aging appear overlapped. Discrimination of these two dimensions would powerfully contribute to a better understanding of the etiology of some neurodegenerative diseases, such as "sporadic" Alzheimer. Methods: Following a system biology approach, top-down and bottom-up strategies were combined. First, public transcriptome data corresponding to the transition from adulthood to the aging stage in normal, human hippocampus were analyzed through an optimized microarray post-processing (Q-GDEMAR method) together with a proper experimental design (full factorial analysis)...
2016: Frontiers in Aging Neuroscience
Jürgen Böhm, Frank Pianka, Nina Stüttgen, Junghyun Rho, Biljana Gigic, Yuzheng Zhang, Nina Habermann, Petra Schrotz-King, Clare Abbenhardt-Martin, Lin Zielske, Paul D Lampe, Alexis Ulrich, Markus K Diener, Cornelia M Ulrich
BACKGROUND: Ventral incisional hernia is the most common long-term complication after an abdominal operation. Among newly diagnosed colorectal cancer patients, we screened the preoperative plasma proteome to explore predictive markers for the development of an incisional hernia. METHODS: We utilized preoperative plasma samples of 72 newly diagnosed colorectal cancer patients who underwent midline incision for tumor resection between 2010 and 2013. A total of 21 patients with incisional hernia occurrence were matched with 51 patients with at least 18 months follow-up without an incisional hernia by sex, age, and body mass index...
October 13, 2016: Surgery
Carmela Nardelli, Ilaria Granata, Laura Iaffaldano, Valeria D'Argenio, Valentina Del Monaco, Giuseppe Maria Maruotti, Luigi Del Vecchio, Pasquale Martinelli, Francesco Salvatore, Mario Rosario Guarracino, Lucia Sacchetti, Lucio Pastore
Experimental evidence indicates differences between women and men in several medical areas, including susceptibility to metabolic diseases. Sexual dimorphism could be influenced by microRNA (miRNA)-mediated regulation of gene expression, and miRNAs have also been implicated in fetal programming of obesity. Our previous finding of altered proteome in human amniotic stem cells (hA-MSCs) during obesity (Ob-) prompted us to look for gender-related differences in the miRNA regulation of gene expression in Ob-hA-MSCs that might be involved in metabolic changes...
October 5, 2016: Stem Cells and Development
Qin Qiao, Séverine Le Manach, Benoit Sotton, Hélène Huet, Evelyne Duvernois-Berthet, Alain Paris, Charlotte Duval, Loïc Ponger, Arul Marie, Alain Blond, Lucrèce Mathéron, Joelle Vinh, Gérard Bolbach, Chakib Djediat, Cécile Bernard, Marc Edery, Benjamin Marie
Sexual dimorphism describes the features that discriminate between the two sexes at various biological levels. Especially, during the reproductive phase, the liver is one of the most sexually dimorphic organs, because of different metabolic demands between the two sexes. The liver is a key organ that plays fundamental roles in various physiological processes, including digestion, energetic metabolism, xenobiotic detoxification, biosynthesis of serum proteins, and also in endocrine or immune response. The sex-dimorphism of the liver is particularly obvious in oviparous animals, as the female liver is the main organ for the synthesis of oocyte constituents...
2016: Scientific Reports
Stefania Ghisaura, Barbara Loi, Grazia Biosa, Maura Baroli, Daniela Pagnozzi, Tonina Roggio, Sergio Uzzau, Roberto Anedda, Maria Filippa Addis
We report the proteomic dataset of gonads from wild Paracentrotus lividus related to the research article entitled "Proteomic changes occurring along gonad maturation in the edible sea urchin Paracentrotus lividus" [1]. Gonads of three individuals per sex in the recovery, pre-mature, mature, and spent stages were analyzed using a shotgun proteomics approach based on filter-aided sample preparation followed by tandem mass spectrometry, protein identification carried out using Sequest-HT as the search engine within the Proteome Discoverer informatics platform, and label-free differential analysis...
September 2016: Data in Brief
Irene Izquierdo, Isaac Rosa, Susana Belén Bravo, Esteban Guitián, Alexandra Pérez-Serra, Oscar Campuzano, Ramon Brugada, Alipio Mangas, Ángel García, Rocio Toro
UNLABELLED: Dilated cardiomyopathy (DCM) is a severe heart disease characterized by progressive ventricular dilation and impaired systolic function of the left ventricle. We recently identified a novel pathogenic mutation in the LMNA gene in a family affected by DCM showing sudden death background. We now aimed to identify potential biomarkers of disease status, as well as sudden death predictors, in members of this family. We analysed plasma samples from 14 family members carrying the mutation, four of which (with relevant clinical symptoms) were chosen for the proteomic analysis...
October 4, 2016: Journal of Proteomics
Jin Hyoung Cho, Jin Young Jeong, Ra Ham Lee, Mi Na Park, Seok-Ho Kim, Seon-Min Park, Jae-Cheon Shin, Young-Joo Jeon, Jung-Hyun Shim, Nag-Jin Choi, Kang Seok Seo, Young Sik Cho, MinSeok S Kim, Sungho Ko, Jae-Min Seo, Seung-Youp Lee, Jung-Il Chae, Hyun-Jeong Lee
Adipose tissue in the loin muscle area of beef cattle as a marbling factor is directly associated with beef quality. To elucidate whether properties of proteins involved in depot specific adipose tissue were sex-dependent, we analyzed protein expression of intramuscular adipose tissue (IMAT) and omental adipose tissue (OMAT) from Hanwoo cows, steers, and bulls of Korean native beef cattle by liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomic analysis, quantitative polymerase chain reaction (PCR) and western blot analysis...
August 2016: Asian-Australasian Journal of Animal Sciences
Chris G C Jacobs, Sandra Steiger, David G Heckel, Natalie Wielsch, Andreas Vilcinskas, Heiko Vogel
The burying beetle Nicrophorus vespilloides has emerged as a model system for the investigation of adaptations that allow the utilization of carrion as a diet and as a resource for reproduction. The survival of beetles and their offspring given their exposure to soil-dwelling and cadaver-borne microbes requires mechanisms that reduce bacterial contamination in the diet and that achieve sanitation of the microhabitat. To explore the role of antimicrobial peptides (AMPs) in this context, we analyzed burying beetle males and females at different stages of their breeding cycle using the RNA-Seq and proteomics approaches...
2016: Scientific Reports
Liming Shen, Youjiao Chen, Aochu Yang, Cheng Chen, Liping Liao, Shuiming Li, Ming Ying, Jing Tian, Qiong Liu, Jiazuan Ni
Oxidative stress is a key event in the onset and progression of neurodegenerative diseases, including Alzheimer's disease (AD). To investigate the role of oxidative stress in AD and to search for potential biomarkers in peripheral blood, serums were collected in this study from the 3-, 6-, and 12-month-old triple transgenic AD mice (3×Tg-AD mice) and the age- and sex-matched non-transgenic (non-Tg) littermates. The serum oxidized proteins were quantified by slot-blot analysis and enzyme-linked immunosorbent assay (ELISA) to investigate the total levels of serum protein carbonyl groups...
2016: International Journal of Molecular Sciences
Min-Dan Xu, Xian-Zheng Wu, Yun Zhou, Ying Xue, Ke-Qin Zhang
OBJECTIVE: This study aimed to evaluate the proteomic characteristics of plasma microparticles (MPs) from patients with newly diagnosed type 2 diabetes (T2DM). METHODS: The subjects comprised eight male T2DM patients recruited between December 2013 and March 2014, as well as eight age and sex-matched healthy controls enrolled during the same period. Plasma microparticles (MPs) were extracted from the blood of each subject, and subjected to proteomics analysis using label-free methods...
2016: American Journal of Translational Research
Denina B D Simmons, James P Sherry
There are questions about the potential for oil sands related chemicals to enter the Athabasca River, whether from tailing ponds, atmospheric deposition, precipitation, or transport of mining dust, at concentrations sufficient to negatively impact the health of biota. We applied shotgun proteomics to generate protein profiles of mature male and female White Sucker (Catostomus commersonii) that were collected from various sites along the main stem of the Athabasca River in 2011 and 2012. On average, 399±131 (standard deviation) proteins were identified in fish plasma from each location in both years...
September 2016: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
Nikolaos Tsotakos, David S Phelps, Christopher M Yengo, Vernon M Chinchilli, Joanna Floros
BACKGROUND: Surfactant protein A (SP-A) contributes to lung immunity by regulating inflammation and responses to microorganisms invading the lung. The huge genetic variability of SP-A in humans implies that this protein is highly important in tightly regulating the lung immune response. Proteomic studies have demonstrated that there are differential responses of the macrophages to SP-A1 and SP-A2 and that there are sex differences implicated in these responses. METHODS: Purified SP-A variants were used for administration to alveolar macrophages from SP-A knockout (KO) mice for in vitro studies, and alveolar macrophages from humanized SP-A transgenic mice were isolated for ex vivo studies...
2016: Biology of Sex Differences
Xavier Gallart-Palau, Benjamin S T Lee, Sunil S Adav, Jingru Qian, Aida Serra, Jung Eun Park, Mitchell K P Lai, Christopher P Chen, Raj N Kalaria, Siu Kwan Sze
BACKGROUND: Dementia risk in women is higher than in men, but the molecular neuropathology of this gender difference remains poorly defined. In this study, we used unbiased, discovery-driven quantitative proteomics to assess the molecular basis of gender influences on risk of Alzheimer's disease with cerebrovascular disease (AD + CVD). RESULTS: We detected modulation of several redox proteins in the temporal lobe of AD + CVD subjects, and we observed sex-specific alterations in the white matter (WM) and mitochondria proteomes of female patients...
2016: Molecular Brain
André Zelanis, Milene C Menezes, Eduardo S Kitano, Tarcísio Liberato, Alexandre K Tashima, Antonio F M Pinto, Nicholas E Sherman, Paulo L Ho, Jay W Fox, Solange M T Serrano
UNLABELLED: Variation in the snake venom proteome is a well-documented phenomenon; however, sex-based variation in the venom proteome/peptidome is poorly understood. Bothrops jararaca shows significant sexual size dimorphism and here we report a comparative proteomic/peptidomic analysis of venoms from male and female specimens and correlate it with the evaluation of important venom features. We demonstrate that adult male and female venoms have distinct profiles of proteolytic activity upon fibrinogen and gelatin...
April 29, 2016: Journal of Proteomics
Nicholas J Hunt, Leo Phillips, Karen A Waters, Rita Machaalani
UNLABELLED: Matrix assisted laser desorption/ionisation imaging mass spectrometry (MALDI-IMS) has not previously been utilised to examine sudden infant death syndrome (SIDS). This study aimed to optimise MALDI IMS for use on archived formalin-fixed-paraffin-embedded human infant medulla tissue (n=6, controls; n=6, SIDS) to evaluate differences between multiple nuclei of the medulla by using high resolution IMS. Profiles were compared between SIDS and age/sex matched controls. LC-MALDI identified 55 proteins based on 321 peptides across all samples; 286 peaks were found using IMS, corresponding to these 55 proteins that were directly compared between controls and SIDS...
April 14, 2016: Journal of Proteomics
George R Saade, Kim A Boggess, Scott A Sullivan, Glenn R Markenson, Jay D Iams, Dean V Coonrod, Leonardo M Pereira, M Sean Esplin, Larry M Cousins, Garrett K Lam, Matthew K Hoffman, Robert D Severinsen, Trina Pugmire, Jeff S Flick, Angela C Fox, Amir J Lueth, Sharon R Rust, Emanuele Mazzola, ChienTing Hsu, Max T Dufford, Chad L Bradford, Ilia E Ichetovkin, Tracey C Fleischer, Ashoka D Polpitiya, Gregory C Critchfield, Paul E Kearney, J Jay Boniface, Durlin E Hickok
BACKGROUND: Preterm delivery remains the leading cause of perinatal mortality. Risk factors and biomarkers have traditionally failed to identify the majority of preterm deliveries. OBJECTIVE: To develop and validate a mass spectrometry-based serum test to predict spontaneous preterm delivery in asymptomatic pregnant women. STUDY DESIGN: A total of 5501 pregnant women were enrolled between 17(0/7) and 28(6/7) weeks gestational age in the prospective Proteomic Assessment of Preterm Risk study at 11 sites in the United States between 2011 and 2013...
May 2016: American Journal of Obstetrics and Gynecology
Sheng Zhang, Yunxiang Zhang, Yanchun Cao, Yanbao Lei, Hao Jiang
Previous studies have shown that there are significant sexual differences in the morphological and physiological responses of Populus cathayana Rehder to nitrogen and phosphorus deficiencies, but little is known about the sex-specific differences in responses to iron deficiency. In this study, the effects of iron deficiency on the morphology, physiology, and proteome of P. cathayana males and females were investigated. The results showed that iron deficiency (25 days) significantly decreased height growth, photosynthetic rate, chlorophyll content, and tissue iron concentration in both sexes...
March 4, 2016: Journal of Proteome Research
Alexandra Dumitriu, Javad Golji, Adam T Labadorf, Benbo Gao, Thomas G Beach, Richard H Myers, Kenneth A Longo, Jeanne C Latourelle
BACKGROUND: Parkinson disease (PD) is a neurodegenerative disease characterized by the accumulation of alpha-synuclein (SNCA) and other proteins in aggregates termed "Lewy Bodies" within neurons. PD has both genetic and environmental risk factors, and while processes leading to aberrant protein aggregation are unknown, past work points to abnormal levels of SNCA and other proteins. Although several genome-wide studies have been performed for PD, these have focused on DNA sequence variants by genome-wide association studies (GWAS) and on RNA levels (microarray transcriptomics), while genome-wide proteomics analysis has been lacking...
2016: BMC Medical Genomics
Qin Shao, Jonathan Fallica, Kevin M Casin, Elizabeth Murphy, Charles Steenbergen, Mark J Kohr
Premenopausal women exhibit endogenous cardioprotective signaling mechanisms that are thought to result from the beneficial effects of estrogen, which we have shown to increase protein S-nitrosylation in the heart. S-nitrosylation is a labile protein modification that increases with a number of different forms of cardioprotection, including ischemic preconditioning. Herein, we sought to identify a potential role for protein S-nitrosylation in sex-dependent cardioprotection. We utilized a Langendorff-perfused mouse heart model of ischemia-reperfusion injury with male and female hearts, and S-nitrosylation-resin-assisted capture with liquid chromatography tandem mass spectrometry to identify S-nitrosylated proteins and modification sites...
February 15, 2016: American Journal of Physiology. Heart and Circulatory Physiology
Sun Eun Lee, Keith P West, Robert N Cole, Kerry J Schulze, Parul Christian, Lee Shu-Fune Wu, James D Yager, John Groopman, Ingo Ruczinski
Inflammation is a condition stemming from complex host defense and tissue repair mechanisms, often simply characterized by plasma levels of a single acute reactant. We attempted to identify candidate biomarkers of systemic inflammation within the plasma proteome. We applied quantitative proteomics using isobaric mass tags (iTRAQ) tandem mass spectrometry to quantify proteins in plasma of 500 Nepalese children 6-8 years of age. We evaluated those that co-vary with inflammation, indexed by α-1-acid glycoprotein (AGP), a conventional biomarker of inflammation in population studies...
2015: PloS One
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