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https://www.readbyqxmd.com/read/28346318/predictors-of-impaired-hdl-function-in-hiv-1-infected-compared-to-uninfected-individuals
#1
Theodoros Kelesidis, Michael N Oda, Mark S Borja, Yumin Yee, Kit F Ng, Diana Huynh, David Elashoff, Judith S Currier
OBJECTIVE: HDL function rather than absolute level may be a more accurate indicator for cardiovascular disease (CVD). Novel methods can measure HDL function using patient samples. The objective of this study is to identify factors that may contribute to HDL dysfunction in chronic, treated HIV-1 infection. DESIGN: Retrospective study of HDL function measured in two ways in HIV-1 infected males with low overall CVD risk and healthy males with no known CVD risk matched by race to the HIV-1 infected participants...
March 23, 2017: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/28345534/electron-transfer-processes-occurring-on-platinum-neural-stimulating-electrodes-calculated-charge-storage-capacities-are-inaccessible-during-applied-stimulation
#2
Eric M Hudak, Doe Kumsa, Heidi Martin, J Mortimer
<i>Objective.</i> Neural prostheses employing platinum electrodes are often constrained by a charge/charge-density parameter known as the Shannon limit. In examining the relationship between charge injection and observed tissue damage, the electrochemistry at the electrode-tissue interface should be considered. The charge-storage capacity (CSC) is often used as a predictor of how much charge an electrode can inject during stimulation, but calculating charge from a steady-state i-E curve (cyclic voltammogram) over the water window misrepresents how electrodes operate during stimulation...
March 27, 2017: Journal of Neural Engineering
https://www.readbyqxmd.com/read/28345378/probing-the-behavior-of-bovine-serum-albumin-upon-binding-to-atenolol-insights-from-spectroscopic-and-molecular-docking-approaches
#3
Tuo-Ying Jiang, Kai-Li Zhou, Yan-Yue Lou, Dong-Qi Pan, Jie-Hua Shi
Molecular interaction of atenolol, a selective β1 receptor antagonist with the major carrier protein, bovine serum albumin (BSA), was investigated under imitated physiological conditions (pH 7.4) by means of fluorescence spectroscopy, UV absorption spectroscopy, Fourier transform infrared spectroscopy (FT-IR), and molecular modeling studies. The steady-state fluorescence spectra manifested that static type, due to formation of the atenolol-BSA complex, was the dominant mechanism for fluorescence quenching...
March 27, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/28344412/clinical-and-hematobiochemical-response-in-canine-monocytic-ehrlichiosis-seropositive-dogs-of-punjab
#4
Manasa R Kottadamane, Pritpal Singh Dhaliwal, Lachhman Das Singla, Baljinder Kumar Bansal, Sanjeev Kumar Uppal
AIM: As in India especially, the Punjab state sero-prevalence and distribution of ehrlichiosis in relation to clinico-hematobiochemical response remains largely unexplored. Thus, this study was designed to determine the prevalence of vector (tick)-borne tropical canine pancytopenia caused by Ehrlichia canis through enzyme labeled ImmunoComb(®) (IC) assay in dogs from in and around Ludhiana, Punjab. Correlation of prevalence was made with various clinico-hematobiochemical parameters. MATERIALS AND METHODS: Seroprevalence study was carried out using IC(®) test kit (Biogal, Galed Labs)...
February 2017: Veterinary World
https://www.readbyqxmd.com/read/28344400/sex-related-differences-in-hemato-biochemical-indices-of-adult-vanaraja-chickens-during-summer-and-winter-seasons
#5
Kuldeep Kumar Panigrahy, Kumaresh Behera, Lal Mohan Mohapatra, Aditya Prasad Acharya, Kamdev Sethy, Sasmita Panda, Shailesh Kumar Gupta
AIM: The objective of this study was to evaluate the changes in hemato-biochemical indices in male and female Vanaraja chickens under tropical environment during summer and winter season. MATERIALS AND METHODS: A total of 120 day-old sexed Vanaraja chicks were selected as experimental chickens and distributed equally in two groups having 60 female and 60 male chickens in each group, respectively. The experiment was continued for 8 weeks (56 days) and both male and female chickens were slaughtered by cervical dislocation method...
February 2017: Veterinary World
https://www.readbyqxmd.com/read/28343107/phosphorylated-tyrosine-based-pseudobioaffinity-adsorbent-for-the-purification-of-immunoglobulin-g
#6
Gisele Luiza Pavan, Igor Tadeu Lazzarotto Bresolin, Angélica Grespan, Sonia Maria Alves Bueno
The present study evaluated the phosphorylated-tyrosine (P-Tyr) based pseudobioaffinity adsorbent for the purification of human immunoglobulin G (IgG). P-Tyr was selected as a ligand to mimic the natural interactions that occur between the immunoreceptor tyrosine-based activation motif and the IgG. The ligand was coupled to bisoxirane-activated agarose gel and the effect of buffer system, pH, and conductivity was performed to elucidate the nature of IgG-P-Tyr interactions. P-Tyr-agarose was able to purify IgG from human plasma solution in HEPES buffer at pH 7...
March 21, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28342847/na-k-atpase-interaction-with-methylglyoxal-as-reactive-metabolic-side-product
#7
Marika Svrckova, Martina Zatloukalova, Petra Dvorakova, Dominika Coufalova, David Novak, Lenka Hernychova, Jan Vacek
Proteins are subject to oxidative modification and the formation of adducts with a broad spectrum of reactive species via enzymatic and non-enzymatic mechanisms. Here we report that in vitro non-enzymatic methylglyoxal (MGO) binding causes the inhibition and formation of MGO advanced glycation end-products (MAGEs) in Na(+)/K(+)-ATPase (NKA). Concretely, MGO adducts with NKA amino acid residues (mainly Arg) and N(ε)-(carboxymethyl)lysine (CML) formation were found. MGO is not only an inhibitor for solubilized NKA (IC50 = 91±16 μM), but also for reconstituted NKA in the lipid bilayer environment, which was clearly demonstrated using a DPPC/DPPE liposome model in the presence or absence of the NKA-selective inhibitor ouabain...
March 22, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28340769/development-of-a-nano-sio2-based-enzyme-linked-ligand-binding-assay-for-the-determination-of-ibuprofen-in-human-urine
#8
Qian-Long Wang, Jing Xie, Xing-De Li, Li-Sheng Ding, Jian Liang, Pei Luo, Lin-Sen Qing
The application domains of classic enzyme-linked ligand binding assay (ELBA) is relatively narrow due to the high cost and hardly available binding receptor. In here, we described for the first time the possibility of developing a new ELBA based on silica nanoparticles (nano-SiO2) to assess the ibuprofen in human urine. Nano-SiO2 with a large surface area was introduced as stationary phase to improve the analytical performance. In the experiment, a competitively binding procedure with human serum albumin (HSA) was performed between the ibuprofen presented in sample and horseradish peroxidase labeled ibuprofen (HRP-ibuprofen) subsequently added...
May 15, 2017: Talanta
https://www.readbyqxmd.com/read/28340058/predictive-modeling-for-monitoring-egg-freshness-during-variable-temperature-storage-conditions
#9
S M Yimenu, J Y Kim, J Koo, B S Kim
The overall aim of this research was to develop egg freshness prediction models in terms of selected quality indices. Six experiments (4 constant temperatures and 2 variable temperatures) were carried out on hen eggs for a total period of 10, 21, 26, 13, and 105 d at storage temperatures of 30, 20, 20 to 10, 30 to 10, and 5 and 10°C, to observe trends in the relative weight loss (RWL), Haugh unit (HU), yolk index (YI), albumin index (AI), yolk pH, and albumin pH. The results showed that there was an increasing trend in the RWL and a decreasing trend in the YI, AI, and HU for all temperature conditions...
March 2, 2017: Poultry Science
https://www.readbyqxmd.com/read/28339557/deficiency-of-circulating-progenitor-cells-associated-with-vascular-thrombosis-of-hemodialysis-patients
#10
Mu-Yang Hsieh, Tsung-Yan Chen, Lin Lin, Shao-Yuan Chuang, Shing-Jong Lin, Der-Cheng Tarng, Po-Hsun Huang, Chih-Cheng Wu
Background.: Hemodialysis (HD) patients have an increased risk of thrombosis. Endothelial progenitor cells (EPCs), which function in vascular repair, are deficient in HD patients. Nonetheless, the relationship between EPC deficiency and thrombosis in HD patients is unknown. Methods.: From January 2010 to December 2012, circulating levels of EPCs that were positive for CD34 and kinase insert domain receptor (KDR) were measured in 269 HD patients. Patients received prospective follow-ups at 6-month intervals until May 2015...
January 17, 2017: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/28339520/efficacy-of-bacillus-subtilis-and-bacitracin-methylene-disalicylate-on-growth-performance-digestibility-blood-metabolites-immunity-and-intestinal-microbiota-after-intramuscular-inoculation-with-escherichia-coli-in-broilers
#11
M Manafi, S Khalaji, M Hedayati, N Pirany
This experiment was conducted to evaluate the effect of Bacillus subtilis (BS) on broiler performance and health after intramuscular inoculation with E. coli and compare its effect with a growth promoter antibiotic. In a completely randomized design manner, 360 male Ross 308 chicks were divided into 6 treatments and 5 replicates of 12 chicks in each replicate. Experimental treatments included control diet, control + E. coli (0.5 mL of culture containing 108 CFU of E. coli/ml), control + 0.1% BS, control + 0...
October 6, 2016: Poultry Science
https://www.readbyqxmd.com/read/28336870/evidence-of-protein-adsorption-in-pegylated-liposomes-influence-of-liposomal-decoration
#12
Marc Sangrà, Joan Estelrich, Raimon Sabaté, Alba Espargaró, Maria Antònia Busquets
In order to contribute to a better knowledge of the events involved in the formation of the protein corona when nanoparticles (NPs) come in contact with proteins, we report a study about the changes on the physicochemical properties of pristine, PEGylated and Cyclic Arginine-Glycine-Aspartate peptide (RGD)-functionalized large unilamelar liposomes (LUVs) or magnetoliposomes (MLs) upon incubation with Bovine Serum Albumin (BSA). The main phospholipid component of both LUVs and MLs was l-α-phosphatydylcholine (PC) or 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) with 20% of cholesterol...
February 10, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28335671/the-relationship-between-survival-rate-and-intradialytic-blood-pressure-changes-in-maintenance-hemodialysis-patients
#13
Jiayue Lu, Minxia Zhu, Shang Liu, Mingli Zhu, Huihua Pang, Xinghui Lin, Zhaohui Ni, Jiaqi Qian, Hong Cai, Weiming Zhang
OBJECTIVE: The objective of this study is to investigate the relationship between blood pressure changes and all-cause mortality, and between blood pressure changes and cardiovascular mortality, for maintenance hemodialysis (MHD) patients during dialysis. METHODS: Data regarding general condition, biochemical indices, and survival prognosis of MHD patients who were treated at the Shanghai Jiao Tong University School of Medicine-affiliated Renji Hospital from July 2007 to December 2012 were collected, in order to evaluate the relationship between patients' blood pressure changes during hemodialysis and mortality...
November 2017: Renal Failure
https://www.readbyqxmd.com/read/28335264/antimicrobial-properties-of-biofunctionalized-silver-nanoparticles-on-clinical-isolates-of-streptococcus-mutans-and-its-serotypes
#14
Ángel Manuel Martínez-Robles, Juan Pablo Loyola-Rodríguez, Norma Verónica Zavala-Alonso, Rita Elizabeth Martinez-Martinez, Facundo Ruiz, René Homero Lara-Castro, Alejandro Donohué-Cornejo, Simón Yobanny Reyes-López, León Francisco Espinosa-Cristóbal
(1) Background: Streptococcus mutans (S. mutans) is the principal pathogen involved in the formation of dental caries. Other systemic diseases have also been associated with specific S. mutans serotypes (c, e, f, and k). Silver nanoparticles (SNP) have been demonstrated to have good antibacterial effects against S. mutans; therefore, limited studies have evaluated the antimicrobial activity of biofunctionalized SNP on S. mutans serotypes. The purpose of this work was to prepare and characterize coated SNP using two different organic components and to evaluate the antimicrobial activity of SNP in clinical isolates of S...
July 22, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28335244/human-serum-albumin-nanoparticles-for-use-in-cancer-drug-delivery-process-optimization-and-in-vitro-characterization
#15
Nikita Lomis, Susan Westfall, Leila Farahdel, Meenakshi Malhotra, Dominique Shum-Tim, Satya Prakash
Human serum albumin nanoparticles (HSA-NPs) are widely-used drug delivery systems with applications in various diseases, like cancer. For intravenous administration of HSA-NPs, the particle size, surface charge, drug loading and in vitro release kinetics are important parameters for consideration. This study focuses on the development of stable HSA-NPs containing the anti-cancer drug paclitaxel (PTX) via the emulsion-solvent evaporation method using a high-pressure homogenizer. The key parameters for the preparation of PTX-HSA-NPs are: the starting concentrations of HSA, PTX and the organic solvent, including the homogenization pressure and its number cycles, were optimized...
June 15, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28333064/the-removal-of-typical-pollutants-in-secondary-effluent-by-the-combined-process-of-powdered-activated-carbon-ultrafiltration
#16
Lihua Sun, Ning He, Tianmin Yu, Xi Duan, Cuimin Feng, Yajun Zhang
This paper focused on the effects of powdered activated carbon (PAC) dosage on ultrafiltration (UF) membrane flux caused by natural organic matter (NOM). Three model foulants, humic acid (HA), bovine serum albumin (BSA) and sodium alginate (SA), were adopted to represent different NOM fractions in secondary effluent treated by the combined process of PAC-UF. Moreover, the membrane fouling resistance and fouling mechanism were also analyzed. The results indicated that the best PAC dosage for the membrane flux variation was 20 mg/L for HA and SA, and 10 mg/L for BSA...
March 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28332473/correlation-analysis-of-renal-ultrasound-elastography-and-clinical-and-pathological-changes-in-patients-with-chronic-kidney-disease%C3%A2
#17
Lingyan Peng, Tingting Zhong, Qiuling Fan, Yanjun Liu, Xuemei Wang, Lining Wang
OBJECTIVE: To analyze the correlations of renal tissue elastography with clinical biochemical indicators and pathological changes in patients with chronic kidney disease (CKD) as well as to explore the potential for renal tissue elastography as a new, noninvasive method for the dynamic monitoring of renal disease progression, efficacy assessment, and prognosis evaluation. METHODS: Patients admitted to the Department of Nephrology of the First Affiliated Hospital of China Medical University from August 2014 to January 2015 who had undergone renal biopsies were selected...
March 23, 2017: Clinical Nephrology
https://www.readbyqxmd.com/read/28331937/whey-protein-and-albumin-effects-upon-urinary-risk-factors-for-stone-formation
#18
Camila Mithie Hattori, Hans-Göran Tiselius, Ita Pfeferman Heilberg
Protein supplements are consumed for an expected increase in muscle mass and improved exercise performance, but as their impact on lithogenic parameters are unknown, we aimed to evaluate the effects of Whey protein (WP) and Albumin upon the risk factors for nephrolithiasis. WP or Albumin supplements (one scoop/day) were administered for 3 days to 18 healthy volunteers, with 1-week washout period between them. Serum and 24-h urine samples were collected at baseline and after completing each intervention. All participants were asked to replicate their baseline diet during the subsequent urine collection...
March 22, 2017: Urolithiasis
https://www.readbyqxmd.com/read/28331312/investigation-of-silk-fibroin-nanoparticle-decorated-poly-l-lactic-acid-composite-scaffolds-for-osteoblast-growth-and-differentiation
#19
Biao-Qi Chen, Ranjith Kumar Kankala, Ai-Zheng Chen, Ding-Zhu Yang, Xiao-Xia Cheng, Ni-Na Jiang, Kai Zhu, Shi-Bin Wang
Attempts to reflect the physiology of organs is quite an intricacy during the tissue engineering process. An ideal scaffold and its surface topography can address and manipulate the cell behavior during the regeneration of targeted tissue, affecting the cell growth and differentiation significantly. Herein, silk fibroin (SF) nanoparticles were incorporated into poly(l-lactic acid) (PLLA) to prepare composite scaffolds via phase-inversion technique using supercritical carbon dioxide (SC-CO2). The SF nanoparticle core increased the surface roughness and hydrophilicity of the PLLA scaffolds, leading to a high affinity for albumin attachment...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28329340/baseline-characteristics-of-participants-in-the-aspree-aspirin-in-reducing-events-in-the-elderly-study
#20
John J McNeil, Robyn L Woods, Mark R Nelson, Anne M Murray, Christopher M Reid, Brenda Kirpach, Elsdon Storey, Raj C Shah, Rory S Wolfe, Andrew M Tonkin, Anne B Newman, Jeff D Williamson, Jessica E Lockery, Karen L Margolis, Michael E Ernst, Walter P Abhayaratna, Nigel Stocks, Sharyn M Fitzgerald, Ruth E Trevaks, Suzanne G Orchard, Lawrence J Beilin, Geoffrey A Donnan, Peter Gibbs, Colin I Johnston, Richard H Grimm
Background: There are no primary prevention trials of aspirin with relevant geriatric outcomes in elderly people. ASPirin in Reducing Events in the Elderly (ASPREE) is a placebo-controlled trial of low-dose aspirin that will determine whether 5 years of daily 100-mg enteric-coated aspirin extends disability-free and dementia-free life in a healthy elderly population and whether these benefits outweigh the risks. Methods: Set in primary care, this randomized double-blind placebo-controlled trial has a composite primary endpoint of death, incident dementia or persistent physical disability...
January 20, 2017: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
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