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Lobke Bastings, Johan R Westphal, Catharina C M Beerendonk, Ruud L M Bekkers, Petra L M Zusterzeel, Jan C M Hendriks, Didi D M Braat, Ronald Peek
PURPOSE: Different protocols are being used worldwide for the cryopreservation of human ovarian tissue for fertility preservation purposes. The efficiency and efficacy of the majority of these protocols has not been extensively evaluated, possibly resulting in sub-optimally cryopreserved ovarian tissue. To address the impact of this issue, we assessed the effects of two clinically successful human ovarian tissue slow-freezing cryopreservation procedures on the quality of the cryopreserved tissue...
October 6, 2016: Journal of Assisted Reproduction and Genetics
Jae Hoon Cha, Sun Rim Kim, Hyun Joong Kang, Myung Hwan Kim, Ae Wha Ha, Woo Kyoung Kim
BACKGROUND/OBJECTIVES: Corn silk (CS) extract contains large amounts of maysin, which is a major flavonoid in CS. However, studies regarding the effect of CS extract on cholesterol metabolism is limited. Therefore, the purpose of this study was to determine the effect of CS extract on cholesterol metabolism in C57BL/6J mouse fed high-fat diets. MATERIALS/METHODS: Normal-fat group fed 7% fat diet, high-fat (HF) group fed 25% fat diet, and high-fat with corn silk (HFCS) group were orally administered CS extract (100 mg/kg body weight) daily...
October 2016: Nutrition Research and Practice
Dongqing Guo, Ming Lu, Xihan Hu, Jiajia Xu, Guangjing Hu, Ming Zhu, Xiaowei Zhang, Qin Li, Catherine Cy Chang, Ta-Yuan Chang, Baoliang Song, Ying Xiong, Boliang Li
Acyl-coenzyme A:cholesterol acyltransferases (ACATs) are the exclusive intracellular enzymes that catalyze the formation of cholesteryl/steryl esters (CE/SE). In our previous work, we found that the high-level expression of human ACAT2 gene with the CpG hypomethylation of its whole promoter was synergistically regulated by two transcription factors Cdx2 and HNF1α in the intestine and fetal liver. Here, we first observed that the specific CpG-hypomethylated promoter was correlated with the low expression of human ACAT2 gene in monocytic cell line THP-1...
September 29, 2016: Acta Biochimica et Biophysica Sinica
Elif Tanriverdi, Murat Acat, Guler Ozgul, Kenan Abbasli, Sule Gul, Zehra Yasar, Mustafa Cortuk, Neslihan Akanil Fener, Hasan Akin, Mehmet Akif Ozgul, Erdogan Cetinkaya
No abstract text is available yet for this article.
September 23, 2016: Leukemia & Lymphoma
Wakako Takabe, Yasuomi Urano, Diep-Khanh Ho Vo, Kimiyuki Shibuya, Masaaki Tanno, Hiroaki Kitagishi, Toyoshi Fujimoto, Noriko Noguchi
24(S)-hydroxycholesterol (24S-OHC), which plays an important role in maintaining brain cholesterol homeostasis, has been shown to possess neurotoxicity. We have previously reported that 24S-OHC esterification by acyl-CoA:cholesterol acyltransferase 1 (ACAT1) and the resulting lipid droplet (LD) formation are responsible for 24S-OHC-induced cell death. In the present study, we investigate the functional roles of 24S-OHC esters and LD formation in 24S-OHC-induced cell death, and we identify four long-chain unsaturated fatty acids (oleic acid, linoleic acid, arachidonic acid, and docosahexaenoic acid) with which 24S-OHC is esterified in human neuroblastoma SH-SY5Y cells treated with 24S-OHC...
September 19, 2016: Journal of Lipid Research
Barry Jubraj, Sheena Patel, Iram Naseem, Samantha Copp, Dimitrios Karagkounis
BACKGROUND: The Acute Care Assessment Tool (ACAT) was developed as a workplace-based assessment (WPBA) for trainee performance whilst working in acute medicine. Here, we discuss the multi-professional potential of ACAT through a pilot with foundation and senior hospital pharmacists. CONTEXT: The pharmacy profession is engaging meaningfully with foundation training for pharmacists akin to doctor foundation training, and has launched a post-foundation recognition scheme as a route to advanced generalist or specialist practice...
September 14, 2016: Clinical Teacher
Elif Tanrıverdi, Mehmet Akif Özgül, Oğuz Uzun, Şule Gül, Mustafa Çörtük, Zehra Yaşar, Murat Acat, Naciye Arda, Erdoğan Çetinkaya
Tracheobronchial amyloidosis is a rare presentation and accounts for about 1% of benign tumors in this area. The diagnosis of disease is delayed due to nonspecific pulmonary symptoms. Therapeutic approaches are required to control progressive pulmonary symptoms for most of the patients. Herein, we report a case of a 68-year-old man admitted with progressive dyspnea to our institution for further evaluation and management. He was initially diagnosed with and underwent management for bronchial asthma for two years but had persistent symptoms despite optimal medical therapy...
2016: Case Reports in Medicine
Junichi Takano, Kazuya Maeda, Michael B Bolger, Yuichi Sugiyama
Intestinal CYP3A and P-glycoprotein (P-gp) decrease the intestinal absorption of substrate drugs. Since substrate specificity of CYP3A often overlaps that of P-gp, and estimation of their saturability in the intestine is difficult, dose-dependent FaFg (fraction of the administered drugs that reach the portal blood) of substrate drugs and the relative importance of CYP3A and P-gp have not been clarified in many cases. Thus, we tried to establish the universal methodology for predicting the in vivo absorption of several CYP3A and/or P-gp substrates from in vitro assays...
November 2016: Drug Metabolism and Disposition: the Biological Fate of Chemicals
K Müller, H Baumeister, B Abberger
OBJECTIVE: The computer adaptive test ACAT-cardio has been developed in order to screen cardiovascular rehabilitation patients for anxiety. This study aims at investigating the criterion validity, and identifying appropriate cut-off values for its use in cardiac rehabilitation. METHODS: 106 cardiovascular rehabilitation patients were tested for anxiety disorders using the instruments ACAT-cardio and SKID-I. Receiver operating characteristics were employed to analyse the results of 3 stopping rules of the ACAT-cardio...
August 2016: Die Rehabilitation
Tülün Öztürk, Kamil Vural, İbrahim Tuğlu, Ahmet Var, Taner Kurdal, Işıl Aydemir
OBJECTIVE: To evaluate the protective effects of preinjury atenolol (acute v chronic) on apoptosis, contractility, oxidative stress, and inflammatory markers in hypercholesterolemic rats undergoing intestinal ischemia-reperfusion (I/R) injury. DESIGN: Prospective, experimental animal study. SETTING: University laboratory. PARTICIPANTS: Male Wistar rats (n = 32). INTERVENTIONS: Rats were divided into the following 4 groups: 1 group was fed a normal diet (ND) (group ND+NoAT [no atenolol]), and the other 3 groups were fed a high-cholesterol diet (HCD)-group HCD+NoAT, group HCD+ChAT (chronic atenolol, 3 mg/kg/day for 8 weeks), and group HCD+AcAT (acute atenolol, 1...
August 2016: Journal of Cardiothoracic and Vascular Anesthesia
Syed Khund-Sayeed, Ximiao He, Timothy Holzberg, Jun Wang, Divya Rajagopal, Shriyash Upadhyay, Stewart R Durell, Sanjit Mukherjee, Matthew T Weirauch, Robert Rose, Charles Vinson
We evaluated DNA binding of the B-HLH family members TCF4 and USF1 using protein binding microarrays (PBMs) containing double-stranded DNA probes with cytosine on both strands or 5-methylcytosine (5mC) or 5-hydroxymethylcytosine (5hmC) on one DNA strand and cytosine on the second strand. TCF4 preferentially bound the E-box motif (CAN|NTG) with strongest binding to the 8-mer CAG|GTGGT. 5mC uniformly decreases DNA binding of both TCF4 and USF1. The bulkier 5hmC also inhibited USF1 binding to DNA. In contrast, 5hmC dramatically enhanced TCF4 binding to E-box motifs ACAT|GTG and ACAC|GTG, being better bound than any 8-mer containing cytosine...
September 12, 2016: Integrative Biology: Quantitative Biosciences From Nano to Macro
Jun Yang, Chen Ji, Yongrui Wu
Maize transcription factors (TFs) opaque2 (O2) and the O2 heterodimerizing proteins (OHP1 and OHP2) originated from an ancient segmental duplication. The 22-kDa (z1C) and 19-kDa (z1A, z1B, and z1D) α-zeins are the most abundant storage proteins in maize endosperm. O2 is known to regulate α-zein gene expression, but its target motifs in the 19-kDa α-zein gene promoters have not been identified. The mechanisms underlying the regulation of α-zein genes by these TFs are also not well understood. In this study, we found that the O2 binding motifs in the α-zein gene promoters are quite flexible, with ACGT being present in the z1C and z1A promoters and a variant, ACAT, being present in the z1B and z1D promoters...
October 2016: Genetics
Won-Sang Cho, Jeong Eun Kim, Jin Chul Paeng, Minseok Suh, Yong-Il Kim, Hyun-Seung Kang, Young Je Son, Jae Seung Bang, Chang Wan Oh
BACKGROUND: Patients with moyamoya disease are frequently encountered with improved symptoms related to anterior cerebral artery territory (ACAt) and middle cerebral artery territory (MCAt) after bypass surgery at MCAt. OBJECTIVE: To evaluate hemodynamic changes in MCAt and ACAt after bypass surgery in adult moyamoya disease. METHODS: Combined bypass surgery was performed on 140 hemispheres in 126 patients with MCAt symptoms. Among them, 87 hemispheres (62...
July 25, 2016: Neurosurgery
Jean-Philippe Drouin-Chartier, André J Tremblay, Valéry Lemelin, Marie-Claude Lépine, Benoît Lamarche, Patrick Couture
AIM: The objective of the present study was to gain further insight into intestinal cholesterol homeostasis in dyslipidemic men with insulin resistance (IR) by examining the impact of treatment with ezetimibe on the expression of key genes involved in cholesterol synthesis and LDL receptor (R)-mediated uptake of lipoproteins. METHODS: Twenty-five men with dyslipidemia and IR were recruited to participate in this double-blinded, randomized, cross-over, placebo-controlled trial...
July 27, 2016: Diabetes, Obesity & Metabolism
Emilienne Tudor Ngo Sock, Gaétan Mayer, Jean-Marc Lavoie
The purpose of this study was to investigate the effects of three weeks of rosuvastatin (Ros) treatment alone and in combination with voluntary training (Tr) on expression of genes involved in cholesterol metabolism (LDLR, PCSK9, LRP-1, SREBP-2, IDOL, ACAT-2 and HMGCR) in the liver of eight week-old ovariectomized (Ovx) rats. Sprague Dawley rats were Ovx or sham-operated (Sham) and kept sedentary for 8 weeks under a standard diet. Thereafter, rats were transferred for three weeks in running wheel cages for Tr or kept sedentary (Sed) with or without Ros treatment (5mg/kg/day)...
2016: PloS One
M Rozsa, A Ford, L Flicker
BACKGROUND: A cross-sectional survey of all patients reviewed by the aged care assessment team (ACAT) service and memory clinics between 1 January and 31 December 2012. The ACAT catchment included an estimated 14 325 people over the age of 70. AIMS: To determine the numbers and outcomes of assessments for cognitive problems by the ACAT and hospital memory clinics for patients within a single ACAT catchment area. METHODS: Data collected included patient demographics, diagnoses, referral and pharmacological treatment...
September 2016: Internal Medicine Journal
Yi Zeng, Le Zhang, Zhiping Hu, Qidong Yang, Mingming Ma, Baoqiong Liu, Jian Xia, Hongwei Xu, Yunhai Liu, Xiaoping Du
Protein Z (PZ) and factor (F) VII are two important factors in the clotting pathway which have similar structure, linked function and nearby gene sites. The aims of this study were to investigate whether the common variants of PZ and FVII genes are associated with the risk of cerebral hemorrhage (CH) and to explore the combined effects of PZ and FVII polymorphisms for CH risk. We performed genotyping analysis for two single-nucleotide polymorphisms (SNPs) of FVII (rs510317 and rs6046) and three SNPs of PZ (rs2273971, rs3024718 and rs3024731) both in a population-based case-control study and in a family-based association study...
June 2016: Journal of Genetics
Lian-Sheng Qiao, Xian-Bao Zhang, Lu-di Jiang, Yan-Ling Zhang, Gong-Yu Li
Acyl-coenzyme A cholesterol acyltransferase (ACAT) plays an important role in maintaining cellular and organismal cholesterol homeostasis. Two types of ACAT isozymes with different functions exist in mammals, named ACAT-1 and ACAT-2. Numerous studies showed that ACAT-2 selective inhibitors are effective for the treatment of hypercholesterolemia and atherosclerosis. However, as a typical endoplasmic reticulum protein, ACAT-2 protein has not been purified and revealed, so combinatorial ligand-based methods might be the optimal strategy for discovering the ACAT-2 selective inhibitors...
June 21, 2016: Molecular Diversity
N Gobeau, R Stringer, S De Buck, T Tuntland, B Faller
PURPOSE: The aim of this study was to evaluate the oral exposure predictions obtained early in drug discovery with a generic GastroPlus Advanced Compartmental And Transit (ACAT) model based on the in vivo intravenous blood concentration-time profile, in silico properties (lipophilicity, pKa) and in vitro high-throughput absorption-distribution-metabolism-excretion (ADME) data (as determined by PAMPA, solubility, liver microsomal stability assays). METHODS: The model was applied to a total of 623 discovery molecules and their oral exposure was predicted in rats and/or dogs...
September 2016: Pharmaceutical Research
Stefano Spolitu, Sabrina Uda, Stefania Deligia, Alessandra Frau, Maria Collu, Fabrizio Angius, Barbara Batetta
Tumor cells are characterised by a high content of cholesterol esters (CEs), while tumor-bearing patients show low levels of high-density lipoproteins (HDLs). The origin and significance of high CE levels in cancer cell biology has not been completely clarified. Recent evidence that lymphoblastic cells selectively acquire exogenous CE from HDL via the scavenger receptor SR-BI has drawn attention to the additional membrane proteins involved in this pathway. P-glycopotein-MDR1 (P-gp) is a product of the MDR1 gene and confers resistance to antitumor drugs...
2016: American Journal of Cancer Research
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