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https://www.readbyqxmd.com/read/28549210/selecting-sugarcane-genotypes-by-the-selection-index-reveals-high-gain-for-technological-quality-traits
#1
L A Silva, P E Teodoro, L A Peixoto, C Assis, K Gasparini, M H P Barbosa, L L Bhering
Sugarcane (Saccharum sp) is one of the most promising crops and researchers have sought for renewable alternative energy sources to reduce CO2 emission. The study of strategies, which allow breeders in the selection of superior genotypes for many traits simultaneously, is important. Therefore, the objectives of this study were: i) to apply path analysis to better understand the relationship between the lignocellulosic traits and technological quality traits with total recoverable sugars (TRS) and ii) to use several multivariate selection indexes to predict the genetic gain and to select superior genotypes in the sugarcane breeding...
May 25, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28548051/xenon-gas-field-ion-source-from-a-single-atom-tip
#2
Wei-Chiao Lai, Chun-Yueh Lin, Wei-Tse Chang, Po-Chang Li, Tsu-Yi Fu, Chia-Seng Chang, T T Tsong, Ing-Shouh Hwang
Focused ion beam (FIB) systems have become powerful diagnostic and modification tools for nanoscience and nanotechnology. Gas field ion sources (GFISs) built from atomic-size emitters offer the highest brightness among all ion sources and thus can improve the spatial resolution of FIB systems. Here we show that the Ir/W(111) single-atom tip (SAT) can emit high-brightness Xe(+) ion beams with a high current stability. The ion emission current versus extraction voltage was analyzed from 150 K up to 309 K. The optimal emitter temperature for maximum Xe(+) ion emission was ∼150 K and the reduced brightness at the Xe gas pressure of 1 × 10(-4) torr is two to three orders of magnitude higher than that of a Ga liquid metal ion source, and four to five orders of magnitude higher than that of a Xe inductively coupled plasma ion source...
May 26, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28548043/epitaxial-graphene-growth-on-fib-patterned-3c-sic-nanostructures-on-si-111-reducing-milling-damage
#3
Mojtaba Amjadipour, Jennifer MacLeod, Josh Lipton-Duffin, Francesca Iacopi, Nunzio Motta
Epitaxial growth of graphene on SiC is a scalable procedure which does not require any further transfer step, making this an ideal platform for graphene nanostructure fabrication. Focused ion beam (FIB) is a very promising tool for exploring the reduction of the lateral dimension of graphene on SiC to the nanometre scale. However, exposure of graphene to the Ga+ beam causes significant surface damage through amorphisation and contamination, preventing epitaxial graphene growth. In this paper we demonstrate that combining a protective silicon layer with FIB patterning implemented prior to graphene growth can significantly reduce the damage associated with FIB milling...
May 26, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28544769/serum-biomarkers-can-predict-a-change-in-liver-fibrosis-one-year-after-lifestyle-intervention-for-biopsy-proven-nash
#4
Eduardo Vilar-Gomez, Luis Calzadilla-Bertot, Scott L Friedman, Bienvenido Gra-Oramas, Licet Gonzalez-Fabian, Sacha Lazo-Del Vallin, Moises Diago, Leon A Adams
BACKGROUND & AIMS: The dynamic response of serum fibrosis biomarkers to histological changes within the liver following lifestyle intervention (LI) is unknown. We explored relationships between changes in serum biomarkers and liver fibrosis in NASH patients undergoing LI. METHODS: Paired liver biopsies were performed in 261 NASH patients to assess fibrosis change after 1 year of LI. We explored the utility of serum fibrosis markers to predict changes in hepatic fibrosis and developed and internally validated a model for predicting fibrosis improvement in patients with baseline fibrosis...
May 24, 2017: Liver International: Official Journal of the International Association for the Study of the Liver
https://www.readbyqxmd.com/read/28544398/prediction-of-long-term-clinical-outcome-in-a-diverse-chronic-hepatitis-b-population-role-of-the-page-b-score
#5
Willem P Brouwer, Adriaan J van der Meer, Andre Boonstra, Elisabeth P C Plompen, Suzan D Pas, Robert J de Knegt, Rob A de Man, Fiebo J W Ten Kate, Harry L A Janssen, Bettina E Hansen
BACKGROUND & AIMS: An abundance of non-invasive scores have been associated with fibrosis and hepatocellular carcinoma (HCC) development. We aimed to compare the prognostic ability of these scores in relation to liver histology in chronic hepatitis B (CHB) patients. METHODS: Liver biopsies from treatment-naïve CHB patients at one tertiary care centre were scored by a single hepato-pathologist. Laboratory values at liver biopsy were used to calculate the PAGE-B, REACH-B, GAG-HCC, CU-HCC, and FIB-4 scores...
May 24, 2017: Journal of Viral Hepatitis
https://www.readbyqxmd.com/read/28544156/m2bpgi-as-a-potential-diagnostic-tool-of-cirrhosis-in-chinese-patients-with-hepatitis-b-virus-infection
#6
Bin Wei, Shu Feng, Enqiang Chen, Dongdong Li, Tingting Wang, Yu Gou, Tingting Yang, Dongmei Zhang, Chuanmin Tao, Hong Tang
BACKGROUND: M2BPGi is a novel serum glycobiomarker of liver fibrosis. In this study, we aimed to evaluate the efficacy of M2BPGi for predicting liver fibrosis and disease progression in Chinese hepatitis B virus (HBV) infected patients. METHODS: We enrolled 228 HBV infected patients with different status of liver fibrosis diagnosed using FibroScan. We analyzed the diagnostic accuracy of M2BPGi, and compared it with AST-to-platelet ratio (APRI), FIB-4 index, AST to ALT ratio (AAR), and RDW to platelet ratio (RPR)...
May 24, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28543215/fib-4-stage-of-liver-fibrosis-predicts-incident-heart-failure-among-hiv-infected-and-uninfected-patients
#7
Kaku A So-Armah, Joseph K Lim, Vincent Lo Re, Janet P Tate, H Chang Chung-Chou, Adeel A Butt, Cynthia L Gibert, David Rimland, Vincent C Marconi, Matthew B Goetz, Maria C Rodriguez-Barradas, Michael E DeBakey, Matthew J Budoff, Hilary A Tindle, Jeffrey H Samet, Amy C Justice, Matthew S Freiberg
Liver fibrosis is common, particularly in human immunodeficiency virus infected (HIV+) people. HIV+ people have excess congestive heart failure (CHF) risk compared to uninfected people. It remains unknown if stage of liver fibrosis influences the CHF risk or if HIV or hepatitis C virus (HCV) infection modifies this association. Our objectives were to assess whether: 1) stage of liver fibrosis is independently associated with incident CHF; 2) the association between stage of liver fibrosis and incident CHF is modified by HIV/HCV status...
May 24, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28538189/evaluation-of-methods-to-sample-fecal-indicator-bacteria-in-foreshore-sand-and-pore-water-at-freshwater-beaches
#8
Laura J Vogel, Thomas A Edge, Denis M O'Carroll, Helena M Solo-Gabriele, Caitlin S E Kushnir, Clare E Robinson
Fecal indicator bacteria (FIB) are known to accumulate in foreshore beach sand and pore water (referred to as foreshore reservoir) where they act as a non-point source for contaminating adjacent surface waters. While guidelines exist for sampling surface waters at recreational beaches, there is no widely-accepted method to collect sand/sediment or pore water samples for FIB enumeration. The effect of different sampling strategies in quantifying the abundance of FIB in the foreshore reservoir is unclear. Sampling was conducted at six freshwater beaches with different sand types to evaluate sampling methods for characterizing the abundance of E...
May 9, 2017: Water Research
https://www.readbyqxmd.com/read/28537667/mechanism-of-the-high-coagulation-state-of-breast-cancer-tissue-factor
#9
X-K Mi, Q-R Liu, L Zhu, M-X Sang, L-R Guo, B-E Shan
OBJECTIVE: We conducted this study to analyze the mechanism behind the high coagulation state induced by circulating plasma microparticle tissue factor (MP-TF) in patients with breast cancer. PATIENTS AND METHODS: 87 cases of breast cancer patients (10 cases of TNM stage I, 16 cases of II, 32 cases of III, 29 cases of IV; 8 cases of pathological type in situ carcinoma, 15 cases of ductal carcinoma, 64 cases of invasive cancer) were used as the observation group and 20 cases of benign breast lesions were used as the control group to compare MP-TF levels of plasma and coagulation parameters including prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FIB) and D-dimer body (D-D) level and NF-κB signaling pathway index including P50, p65, TAK1 and IκBα levels...
May 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28535787/novel-serum-biomarkers-modified-by-the-body-mass-index-z-score-for-the-detection-of-liver-fibrosis-and-steatosis-in-children-with-chronic-hepatitis-c
#10
Maria Pokorska-Śpiewak, Barbara Kowalik-Mikołajewska, Małgorzata Aniszewska, Magdalena Pluta, Magdalena Marczyńska
BACKGROUND: There is a need for validation of noninvasive alternatives to liver biopsy for the evaluation of fibrosis in children with chronic hepatitis C (CHC). The aim of this study was to evaluate the diagnostic performance of serum biomarkers modified by the body mass index z-score (BMI z-score) for the detection of fibrosis and steatosis in children with CHC. METHODS: Thirty children aged 9.4 ± 3.7 years (14 males, 16 females) with CHC underwent liver biopsy...
May 23, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28528642/minimal-resin-embedding-of-multicellular-specimens-for-targeted-fib-sem-imaging
#11
Nicole L Schieber, Pedro Machado, Sebastian M Markert, Christian Stigloher, Yannick Schwab, Anna M Steyer
Correlative light and electron microscopy (CLEM) is a powerful tool to perform ultrastructural analysis of targeted tissues or cells. The large field of view of the light microscope (LM) enables quick and efficient surveys of the whole specimen. It is also compatible with live imaging, giving access to functional assays. CLEM protocols take advantage of the features to efficiently retrace the position of targeted sites when switching from one modality to the other. They more often rely on anatomical cues that are visible both by light and electron microscopy...
2017: Methods in Cell Biology
https://www.readbyqxmd.com/read/28524028/-efficacy-of-20-albumin-infusion-on-coagulation-and-pulmonary-fibrosis-in-rabbits-with-ards
#12
Ling Yao, Bo Liu, Feng Shen
OBJECTIVE: To observe the efficacy of 20% albumin infusion on coagulation and pulmonary fibrosis in rabbits with acute respiratory distress syndrome (ARDS) induced by two-hit of intravenous infusion of oleid acid (OA) and lipopolysaccharide (LPS). METHODS: Forty healthy adult male rabbits were randomly divided into five groups, namely sham group, model group, albumin group (20% albumin), saline group (0.9% sodium chloride injection) and Ringer group (lactate Ringer solution), with 8 rabbits in each group...
May 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28514753/impact-of-age-on-the-diagnostic-performances-and-cut-offs-of-apri-and-fib-4-for-significant-fibrosis-and-cirrhosis-in-chronic-hepatitis-b
#13
Qiang Li, Chuan Lu, Weixia Li, Yuxian Huang, Liang Chen
AIMS: Assessing the diagnostic performances of APRI and FIB-4 using age as a categorical marker. METHODS: 822 chronic hepatitis B (CHB) patients were included. Using METAVIR scoring system as a reference, the performances of APRI and FIB-4 were compared between patients aged≥30 and patients aged<30 years. RESULTS: The APRI AUROC in patients aged<30 years was lower than that in patients aged≥30 years for significant fibrosis (0.61 vs 0...
April 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/28504417/laser-preparation-of-geometrically-optimised-samples-for-x-ray-nano-ct
#14
J J Bailey, T M M Heenan, D P Finegan, X Lu, S R Daemi, F Iacoviello, N R Backeberg, O O Taiwo, D J L Brett, A Atkinson, P R Shearing
A robust and versatile sample preparation technique for the fabrication of cylindrical pillars for imaging by X-ray nano-computed tomography (nano-CT) is presented. The procedure employs simple, cost-effective laser micro-machining coupled with focused-ion beam (FIB) milling, when required, to yield mechanically robust samples at the micrometre length-scale to match the field-of-view (FOV) for nano-CT imaging. A variety of energy and geological materials are exhibited as case studies, demonstrating the procedure can be applied to a variety of materials to provide geometrically optimised samples whose size and shape are tailored to the attenuation coefficients of the constituent phases...
May 15, 2017: Journal of Microscopy
https://www.readbyqxmd.com/read/28504292/microstructure-degradation-of-ni-cgo-anodes-for-solid-oxide-fuel-cells-after-long-operation-time-using-3d-reconstructions-by-fib-tomography
#15
Atef Zekri, Martin Knipper, Jürgen Parisi, Thorsten Plaggenborg
Solid oxide fuel cells (SOFCs) are electrochemical conversion devices, which essentially consist of two porous electrodes separated by a dense, oxide ion conducting electrolyte. The performance and the durability of SOFCs strongly depend on the electrode microstructure. In this paper, the impact of a relatively long exposure time (up to 20 000 h) under realistic operation terms (temperature (T) = 850 °C, current density (J) = 190-250 mA cm(-2)) in the kinetics of microstructural degradation are investigated for porous nickel (Ni)/ceria gadolinium oxide (CGO) anodes, to understand the microstructural evolution in SOFC cermet anodes...
May 15, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28502670/direct-quantification-of-dual-protein-adsorption-dynamics-in-three-dimensional-systems-in-presence-of-cells
#16
Melika Sarem, Daniel Vonwil, Steffen Lüdeke, V Prasad Shastri
Understanding the composition of the adsorbed protein layer on a biomaterial surface is of an extreme importance as it directs the primary biological response. Direct detection using labeled proteins and indirect detection based on enzymatic assays or changes to mass, refractive index or density of a surface have been so far established. Nevertheless, using current methodologies, detection of multiple proteins simultaneously and particularly in a three-dimensional (3D) substrates is challenging, with the exception of radiolabeling...
May 11, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28499405/a-comparative-molecular-and-3-dimensional-structural-investigation-into-cross-continental-and-novel-avian-trypanosoma-spp-in-australia
#17
Crystal Cooper, R C Andrew Thompson, Adriana Botero, Amanda Kristancic, Christopher Peacock, Yaowanuj Kirilak, Peta L Clode
BACKGROUND: Molecular and structural information on avian Trypanosoma spp. throughout Australia is limited despite their intrinsic value in understanding trypanosomatid evolution, diversity, and structural biology. In Western Australia tissue samples (n = 429) extracted from 93 birds in 25 bird species were screened using generic PCR primers to investigate the diversity of Trypanosoma spp. To investigate avian trypanosome structural biology the first 3-dimensional ultrastructural models of a Trypanosoma spp...
May 12, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28497593/polymorphism-of-receptor-type-tyrosine-protein-phosphatase-delta-gene-in-the-development-of-non-alcoholic-fatty-liver-disease
#18
Shunsuke Nakajima, Hiroki Tanaka, Koji Sawada, Hidemi Hayashi, Takumu Hasebe, Masami Abe, Chitomi Hasebe, Mikihiro Fujiya, Toshikatsu Okumura
BACKGROUND AND AIM: Some single nucleotide polymorphisms (SNPs) are associated with the development of non-alcoholic fatty liver disease (NAFLD). As one of the genetic factors, PNPLA3 rs738409 (I148M) is important to associate with pathogenesis of NAFLD. Since other SNPs remain unclear in Japan, we performed high-throughput sequencing, which targeted more than 1,000 genes to identify a novel genetic variant in Japanese patients with NAFLD. METHODS: The present study in 36 NAFLD patients and 27 healthy volunteers (HVs) was performed...
May 11, 2017: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28487005/nitrogen-gas-field-ion-source-gfis-focused-ion-beam-fib-secondary-electron-imaging-a-first-look
#19
Marek E Schmidt, Anto Yasaka, Masashi Akabori, Hiroshi Mizuta
The recent technological advance of the gas field ion source (GFIS) and its successful integration into systems has renewed the interest in the focused ion beam (FIB) technology. Due to the atomically small source size and the use of light ions, the limitations of the liquid metal ion source are solved as device dimensions are pushed further towards the single-digit nanometer size. Helium and neon ions are the most widely used, but a large portfolio of available ion species is desirable, to allow a wide range of applications...
May 10, 2017: Microscopy and Microanalysis
https://www.readbyqxmd.com/read/28485425/antithrombotic-effect-and-mechanism-of-rubus-spp-blackberry
#20
Pingyao Xie, Yong Zhang, Xuebiao Wang, Jinfeng Wei, Wenyi Kang
The compounds of Rubus spp. Blackberry (RSB) were isolated and identified by a bioassay-guided method, and their antithrombotic effects and mechanism were investigated with the acute blood stasis rat model. The RSB extract was evaluated by activated partial thromboplastin time (APTT), thrombin time (TT), prothrombin time (PT), and fibrinogen (FIB) assays in vitro. Results indicated that RSB extract exhibited anticoagulant activity. In addition to compounds 1 and 6, the other compounds also exhibited anticoagulant activity in vitro...
May 24, 2017: Food & Function
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