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https://www.readbyqxmd.com/read/28723712/the-role-of-oncogenic-hpv-determination-for-diagnosis-of-high-grade-anal-intraepithelial-neoplasia-in-hiv-infected-msm
#1
Joaquin Burgos, J Hernández-Losa, S Landolfi, A Guelar, Mªc Dinares, J Villar, J Navarro, E Ribera, V Falcó, A Curran
OBJECTIVE: To assess the oncogenic human papillomavirus (HPV) determination and the co-testing HPV and anal cytology value to detect high-grade anal intraepithelial neoplasia (HGAIN) in a cohort of HIV-MSM. DESIGN AND METHODS: Prospective study of HIV-infected MSM who underwent screening for anal dysplasia. Screening program includes anal cytology, HPV testing, and high-resolution anoscopy (HRA) at each visit. Histological samples were obtained if suspicious lesions were revealed by HRA...
July 18, 2017: AIDS
https://www.readbyqxmd.com/read/28707725/preventive-effect-of-human-papillomavirus-vaccination-on-the-development-of-uterine-cervical-lesions-in-young-japanese-women
#2
Hidenori Tanaka, Hiromitsu Shirasawa, Dai Shimizu, Naoki Sato, Noriaki Ooyama, Osamu Takahashi, Yukihiro Terada
AIM: To examine the effect that human papillomavirus (HPV) vaccination has had in Akita Prefecture, by comparing cervical cytology results from women aged 20-24 years with or without HPV vaccination. METHODS: We examined cervical cytology results from 2425 subjects who underwent cervical cancer screening under the jurisdiction of the Akita Foundation for Healthcare between January 2014 and October 2016. We compared the prevalence of cytologic abnormalities among women aged 20-24 years with or without HPV vaccination...
July 14, 2017: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/28694293/the-trophic-life-cycle-stage-of-the-opportunistic-fungal-pathogen-pneumocystis-murina-hinders-the-ability-of-dendritic-cells-to-stimulate-cd4-t-cell-responses
#3
Heather M Evans, Andrew Simpson, Shu Shen, Arnold J Stromberg, Carol L Pickett, Beth A Garvy
The life cycle of the opportunistic fungal pathogen Pneumocystis murina consists of a trophic stage and an ascus-like cystic stage. Infection with the cyst stage induces proinflammatory immune responses, while trophic forms suppress the cytokine response to multiple PAMPs, including β-glucan. A targeted gene expression assay was used to evaluate the dendritic cell response following stimulation with trophic forms alone, a normal mixture of trophic forms and cysts, or with β-glucan. We demonstrate that stimulation with trophic forms downregulated the expression of multiple genes normally associated with the response to infection, including genes encoding transcription factors...
July 10, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28692865/live-cell-confocal-laser-imaging-studies-on-the-nuclear-behavior-during-meiosis-and-ascosporogenesis-in-morchella-importuna-under-artificial-cultivation
#4
Peixin He, Ke Wang, Yingli Cai, Wei Liu
The commercial production of Morchella mushrooms, especially M. importuna, has been realized since 2012 in China, which facilitates the fundamental studies of Morchella spp. In this paper, the cytological characteristics at three stages of ascocarpic development and the nuclear behavior during meiosis and ascosporogenesis in cultivated strain M. importuna 1(#) was visualized by confocal laser scanning microscopy. The results suggested that the strain sporulated at sporulation stage of ascocarpic development...
June 29, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28692681/cervical-hpv-type-specific-pre-vaccination-prevalence-and-age-distribution-in-croatia
#5
Ivan Sabol, Nina Milutin Gašperov, Mihaela Matovina, Ksenija Božinović, Goran Grubišić, Ivan Fistonić, Dragan Belci, Laia Alemany, Sonja Džebro, Mara Dominis, Mario Šekerija, Sara Tous, Silvia de Sanjosé, Magdalena Grce
The main etiological factor of precancerous lesion and invasive cervical cancer are oncogenic human papillomaviruses types (HPVs). The objective of this study was to establish the distribution of the most common HPVs in different cervical lesions and cancer prior to the implementation of organized population-based cervical screening and HPV vaccination in Croatia. In this study, 4,432 cervical specimens, collected through a 16-year period, were tested for the presence of HPV-DNA by polymerase chain reaction (PCR) with three sets of broad-spectrum primers and type-specific primers for most common low-risk (LR) types (HPV-6, 11) and the most common high-risk (HR) types (HPV-16, 18, 31, 33, 45, 52, 58)...
2017: PloS One
https://www.readbyqxmd.com/read/28688605/human-papillomavirus-prevalence-in-the-anus-and-urine-among-hiv-infected-japanese-men-who-have-sex-with-men
#6
Hiroshi Yaegashi, Kazuyoshi Shigehara, Ichiro Itoda, Mitsuaki Ohkodo, Kazufumi Nakashima, Shohei Kawaguchi, Mikio Ueda, Koji Izumi, Yoshifumi Kadono, Hiroko Ikeda, Mikio Namiki, Atsushi Mizokami
BACKGROUND: The present study investigated human papillomavirus (HPV) prevalence in anal and urine samples, and evaluated cytological findings among human immunodeficiency virus (HIV)-infected Japanese men who have sex with men (MSM). METHODS: A total of 148 patients were enrolled. Anal and urine samples were collected from each participant, and a HPV-DNA test and genotyping were performed using flow-through hybridization. In addition, anal cytology was evaluated based on Papanicolaou staining...
July 6, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28685798/cyto-colpo-histologic-correlation-about-an-analytical-study-of-120-colposcopies
#7
Olfa Slimani, Riadh Ben Temim, Tahar Makhlouf, Nabil Mathlouthi, Leila Attia
PROBLEM: Cervical cancer is the second gynecological cancer Tunisian women after breast. This is a sexually transmitted disease including the role of HPV has been proven. Cervical cancer screening is possible due to the accessibility of the cervix with a speculum examination and implementation of cervical smear. AIM OF THE WORK: To study the epidemiological and clinical data of patients, analyze the results of the Pap test, colposcopy and cervical biopsy staging are the lesions observed in colposcopy, compare the results of smears, colposcopy and cervical biopsy...
October 2016: La Tunisie Médicale
https://www.readbyqxmd.com/read/28676631/rna-editing-of-the-amd1-gene-is-important-for-ascus-maturation-and-ascospore-discharge-in-fusarium-graminearum
#8
Shulin Cao, Yi He, Chaofeng Hao, Yan Xu, Hongchang Zhang, Chenfang Wang, Huiquan Liu, Jin-Rong Xu
Ascospores are the primary inoculum in the wheat scab fungus Fusarium graminearum that was recently shown to have sexual stage-specific A-to-I RNA editing. One of the genes with premature-stop-codons requiring A-to-I editing to encode full-length functional proteins is AMD1 that encodes a protein with a major facilitator superfamily (MFS) domain. Here, we characterized the functions of AMD1 and its UAG to UGG editing event. The amd1 deletion mutant was normal in growth and conidiation but defective in ascospore discharge due to the premature breakdown of its ascus wall in older perithecia, which is consistent with the specific expression of AMD1 at later stages of sexual development...
July 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28657397/histological-outcomes-in-conventional-cervical-cytology-for-invasive-carcinoma-not-always-cancer
#9
Flávia Regina Peixoto Pereira, Leila Cristina Soares, Marco Aurélio Pinho de Oliveira
The value of cytology for the detection of invasive cancer remains unknown. We performed a retrospective cohort study with 884 patients diagnosed of premalignant lesions and invasive carcinoma in cytology. 382 (43.2%) of them had cytological results of high-grade squamous intraepithelial lesions (HSIL), 244 (27.6%) showed low-grade squamous intraepithelial lesions (LSIL), 120 (13.6%) patients had atypical squamous cells of undetermined significance (ASC-US), 67 (7.6%) patients with atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion (ASC-H), 38 (4...
June 28, 2017: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/28657224/nationwide-cervical-cancer-screening-in-korea-data-from-the-national-health-insurance-service-cancer-screening-program-and-national-cancer-screening-program-2009-2014
#10
Seung Hyuk Shim, Hyeongsu Kim, In Sook Sohn, Han Sung Hwang, Han Sung Kwon, Sun Joo Lee, Ji Young Lee, Soo Nyung Kim, Kunsei Lee, Sounghoon Chang
OBJECTIVE: The rates of participation in the Korean nationwide cervical cancer screening program and the rates of abnormal test results were determined. METHODS: The database of the National Health Insurance Service (NHIS) was used during the study period (2009-2014). RESULTS: The participation rate increased from 41.10% in 2009 to 51.52% in 2014 (annual percentage change, 4.126%; 95% confidence interval [CI]=2.253-6.034). During the study period, women ≥70 years of age had the lowest rate of participation (range, 21...
May 26, 2017: Journal of Gynecologic Oncology
https://www.readbyqxmd.com/read/28655061/outcomes-in-women-with-cytology-showing-atypical-squamous-cells-of-undetermined-significance-with-vs-without-human-papillomavirus-testing
#11
Jack Cuzick, Orrin Myers, Ji-Hyun Lee, Yang Shi, Julia C Gage, William C Hunt, Michael Robertson, Cosette M Wheeler
Importance: Little is known about the long-term yield of high-grade cervical intraepithelial neoplasia (CIN) and the influence on biopsy and treatment rates of human papillomavirus (HPV) triage of cytology showing atypical squamous cells of undetermined significance (hereafter ASC-US cytology). Objective: To examine 5-year outcomes after ASC-US cytology with vs without HPV testing. Design, Setting, and Participants: In this observational study, all cervical cytology and HPV testing reports from January 1, 2007, to December 31, 2012, were obtained for women throughout New Mexico and linked to pathology reports...
June 22, 2017: JAMA Oncology
https://www.readbyqxmd.com/read/28654967/human-papillomavirus-triage-of-women-with-atypical-squamous-cells-of-undetermined-significance-reduction-of-overtreatment-needed
#12
Chris J L M Meijer, Peter J F Snijders
No abstract text is available yet for this article.
June 22, 2017: JAMA Oncology
https://www.readbyqxmd.com/read/28643492/human-papillomavirus-genotype-distribution-among-18-815-women-in-13-korean-cities-and-relationship-with-cervical-cytology-findings
#13
Eun Hee Nah, Seon Cho, Suyoung Kim, Han Ik Cho
BACKGROUND: The prevalence and genotype distribution of Human papillomavirus (HPV) infection vary depending on geographical region and the immunity provided by vaccines. This study aimed to clarify the recent prevalence and genotype distribution of HPV according to age and cervical cytology findings in Korea. METHODS: This study included 18,815 health examinees that underwent cervical cytology and HPV genotyping tests at 16 centers of Korean Association of Health Promotion in 13 cities in Korea, between January 2014 and October 2015...
September 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28619772/cost-effectiveness-of-hpv-based-cervical-cancer-screening-in-the-public-health-system-in-nicaragua
#14
Nicole G Campos, Mercy Mvundura, Jose Jeronimo, Francesca Holme, Elisabeth Vodicka, Jane J Kim
OBJECTIVES: To evaluate the cost-effectiveness of human papillomavirus (HPV) DNA testing (versus Papanicolaou (Pap)-based screening) for cervical cancer screening in Nicaragua. DESIGN: A previously developed Monte Carlo simulation model of the natural history of HPV infection and cervical cancer was calibrated to epidemiological data from Nicaragua. Cost data inputs were derived using a micro-costing approach in Carazo, Chontales and Chinandega departments; test performance data were from a demonstration project in Masaya department...
June 15, 2017: BMJ Open
https://www.readbyqxmd.com/read/28587376/clinical-progress-of-human-papillomavirus-genotypes-and-their-persistent-infection-in-subjects-with-atypical-squamous-cells-of-undetermined-significance-cytology-statistical-and-latent-dirichlet-allocation-analysis
#15
Yee Suk Kim, Sungin Lee, Nansu Zong, Jimin Kahng
The present study aimed to investigate differences in prognosis based on human papillomavirus (HPV) infection, persistent infection and genotype variations for patients exhibiting atypical squamous cells of undetermined significance (ASCUS) in their initial Papanicolaou (PAP) test results. A latent Dirichlet allocation (LDA)-based tool was developed that may offer a facilitated means of communication to be employed during patient-doctor consultations. The present study assessed 491 patients (139 HPV-positive and 352 HPV-negative cases) with a PAP test result of ASCUS with a follow-up period ≥2 years...
June 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28580567/cytohistological-discrepancies-of-cervico-vaginal-smears-and-hpv-status
#16
İbrahim Alanbay, Mustafa Öztürk, Fahri Burçin Fıratlıgil, Kazım Emre Karaşahin, Müfit Cemal Yenen, Serkan Bodur
OBJECTIVES: Discrepancies between abnormal cervical cytology or high-risk human papillomavirus (HR-HPV) status (cytolo-gy negative/HPV positive) and subsequent histological findings are a common occurrence. After using co-testing, the dis-crepancies between the HR-HPV status and cervical cytology have become an issue. In this study, we aimed to determine the characteristics of women with a discrepancy between histology and cytology/HR-HPV status, in terms of diagnosis, review and identification...
2017: Ginekologia Polska
https://www.readbyqxmd.com/read/28535400/comparison-of-the-cervista-hpv-hr-test-and-luminex-xmap-technology-for-the-diagnosis-of-cervical-intraepithelial-neoplasia
#17
Pei-Yu Zhao, Hui-Ci Jiang, Yue Li, Jin-Bo Wang, Ting-Ting Zhang, Cheng-Hua Liu, Li-Wen Song, Jia-Jing Cheng
OBJECTIVES: This study was designed to compare the Cervista high risk (HR) human papillomavirus (HPV) test with Luminex XMAP technology for the detection of the relationship between HPV infection and cervical intraepithelial neoplasia. METHODS: In total, 3280 patients in a cervical specialty clinic were divided into two groups for either Cervista (1855 patients) or Luminex (1425 patients). Subsequent colposcopy examinations were performed in 1270 women with cytologic results showing ASCUS or higher level lesions...
May 16, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28529583/evaluation-of-microrna-205-expression-as-a-potential-triage-marker-for-patients-with-low-grade-squamous-intraepithelial-lesions
#18
Hong Xie, Ingrid Norman, Anders Hjerpe, Tomislav Vladic, Catharina Larsson, Weng-Onn Lui, Ellinor Östensson, Sonia Andersson
High-risk human papillomavirus (HPV) testing is a recommended triage approach for females with atypical squamous cells of undetermined significance (ASCUS), but due to its poor specificity this approach is not recommended for patients with low-grade squamous intraepithelial lesions (LSIL). The objective of the current study was to determine microRNA (miR)-205 expression levels in liquid-based cytology (LBC) samples, and evaluate their ability to predict cervical intraepithelial neoplasia grade 2/3 or worse (CIN2/3+) in females with minor cytological abnormalities...
May 2017: Oncology Letters
https://www.readbyqxmd.com/read/28521328/assessment-of-100-rapid-review-as-an-effective-tool-for-internal-quality-control-in-cytopathological-services
#19
José Queiroz Filho, Janaina Cristiana de Oliveira Crispim Freitas, Daliana Caldas Pessoa, José Eleutério Júnior, Paulo César Giraldo, Ana Katherine Gonçalves
OBJECTIVE: The aim of this study was to evaluate the 100% rapid review (100%-RR) as an effective tool for internal quality control (IQC) in gynecological cytopathology services. STUDY DESIGN: A total of 8,677 swabs were analyzed; the negative results were submitted to 100%-RR. Divergent cases were discussed in a consensus meeting to reach a conclusion on the final diagnosis. The data were entered into SAS statistical software, and the agreement of the 100%-RR results with the final diagnosis was tested with the weighted kappa statistic...
2017: Acta Cytologica
https://www.readbyqxmd.com/read/28511636/hpv-prevalence-and-type-distribution-in-cypriot-women-with-cervical-cytological-abnormalities
#20
George Krashias, Dana Koptides, Christina Christodoulou
BACKGROUND: Human papillomavirus (HPV) is the most common sexually transmitted agent, and it can cause cervical lesions and cancer in females. Currently, information regarding the prevalence of HPV in Cyprus is lacking. The aim of this study was to evaluate the HPV type-specific prevalence in 596 women, aged 19-65 years, with cytological abnormalities. Additionally, in a subset of 348 women for whom cytology results of the Pap test were available, the association between HPV infection and cervical disease was investigated...
May 16, 2017: BMC Infectious Diseases
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