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https://www.readbyqxmd.com/read/27913707/correction-antagonists-of-igf-vitronectin-interactions-inhibit-igf-i-induced-breast-cancer-cell-functions
#1
(no author information available yet)
No abstract text is available yet for this article.
December 2016: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/27896223/urokinase-type-plasminogen-activator-receptor-upar-as-a-new-therapeutic-target-in-cancer
#2
Nunzia Montuori, Ada Pesapane, Francesca W Rossi, Valentina Giudice, Amato De Paulis, Carmine Selleri, Pia Ragno
The urokinase (uPA)-type plasminogen activator receptor (uPAR) is a GPI-anchored receptor that focuses urokinase (uPA) proteolytic activity on the cell surface. uPAR also regulates cell adhesion, migration and proliferation, protects from apoptosis and contributes to epithelial mesenchymal transition (EMT), independently of uPA enzymatic activity. Indeed, uPAR interacts with beta1, beta2 and beta3 integrins, thus regulating their activities. uPAR cross-talks with receptor tyrosine kinases through integrins and regulates cancer cell dormancy, proliferation and angiogenesis...
November 2016: Translational Medicine @ UniSa
https://www.readbyqxmd.com/read/27871448/diagnostic-and-prognostic-value-of-serum-vitronectin-levels-in-human-glioma
#3
Mao-Hua Chen, Chuan Lu, Jun Sun, Xian-Dong Chen, Jun-Xia Dai, Jian-Yong Cai, Xiang-Lin Chen
OBJECTIVE: Vitronectin is an extracellular matrix protein, the synthesis of which by glioma cells correlates with tumor grade. The current study was designed to investigate the relationship between serum vitronectin levels and clinicopathological characteristics, diagnosis and prognosis in glioma patients. METHODS: In a prospective observatory study, a total of 98 glioma patients, 98 healthy controls, 98 other non-glioma brain tumors, and 98 other non-tumor neurological diseases were recruited...
December 15, 2016: Journal of the Neurological Sciences
https://www.readbyqxmd.com/read/27845768/selection-of-a-novel-aptamer-against-vitronectin-using-capillary-electrophoresis-and-next-generation-sequencing
#4
Christopher H Stuart, Kathryn R Riley, Olcay Boyacioglu, Denise M Herpai, Waldemar Debinski, Shadi Qasem, Frank C Marini, Christa L Colyer, William H Gmeiner
Breast cancer (BC) results in ~40,000 deaths each year in the United States and even among survivors treatment of the disease may have devastating consequences, including increased risk for heart disease and cognitive impairment resulting from the toxic effects of chemotherapy. Aptamer-mediated drug delivery can contribute to improved treatment outcomes through the selective delivery of chemotherapy to BC cells, provided suitable cancer-specific antigens can be identified. We report here the use of capillary electrophoresis in conjunction with next generation sequencing to develop the first vitronectin (VN) binding aptamer (VBA-01; Kd 405 nmol/l, the first aptamer to vitronectin (VN; Kd = 405 nmol/l) , a protein that plays an important role in wound healing and that is present at elevated levels in BC tissue and in the blood of BC patients relative to the corresponding nonmalignant tissues...
November 15, 2016: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/27833126/binary-colloidal-crystals-bccs-as-a-feeder-free-system-to-generate-human-induced-pluripotent-stem-cells-hipscs
#5
Peng-Yuan Wang, Sandy Shen-Chi Hung, Helmut Thissen, Peter Kingshott, Raymond Ching-Bong Wong
Human induced pluripotent stem cells (hiPSCs) are capable of differentiating into any cell type and provide significant advances to cell therapy and regenerative medicine. However, the current protocol for hiPSC generation is relatively inefficient and often results in many partially reprogrammed colonies, which increases the cost and reduces the applicability of hiPSCs. Biophysical stimulation, in particular from tuning cell-surface interactions, can trigger specific cellular responses that could in turn promote the reprogramming process...
November 11, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27830022/transplantation-of-bone-marrow-derived-mesenchymal-stem-cells-rescues-partially-rachitic-phenotypes-induced-by-1-25-dihydroxyvitamin-d-deficiency-in-mice
#6
Zengli Zhang, Shaomeng Yin, Xian Xue, Ji Ji, Jian Tong, David Goltzman, Dengshun Miao
To determine whether the transplantation of bone marrow-derived mesenchymal stem cells (BM-MSCs) can improve the 1,25(OH)2D deficiency-induced rachitic phenotype, 2×10(6) BM-MSCs from wild-type mice or vehicle were transplanted by tail vein injection into mice deficient in 1,25(OH)2D due to targeted deletion of 1α(OH)ase (1α(OH)ase(-/-)). Our results show that 1α(OH)ase mRNA was expressed in the BM-MSCs derived from wild-type mice, and was detected in long bone, kidney and intestine from BM-MSC-transplanted 1α(OH)ase(-/-) recipients...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27828845/early-effects-of-paroxysmal-atrial-fibrillation-on-plasma-markers-of-fibrinolysis
#7
Mariya Negreva, Svetoslav Georgiev, Katerina Vitlianova
There are no studies to date on the early changes in the hemostasis profile of patients with paroxysmal atrial fibrillation (PAF).Given the key role of the fibrinolytic system in maintaining blood fluidity, our aim was to examine its activity in patients with clinical manifestation of the disease <24 hours.We studied 51 nonanticoagulated patients with a first episode of the disease (26 men, 25 women; mean age 59.84 ± 1.60 years) and 52 controls (26 men, 26 women; mean age 59.50 ± 1.46 years) who matched the patients in terms of gender, age, comorbidities, and conducted treatment...
November 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27826074/anatomical-differences-of-the-protein-profile-in-the-rabbit-sclera-during-growth
#8
Ying Liu, Pingbo Zhang, Jianbo Pan, Mark Augustine D'Souza, Craig P Dufresne, Richard D Semba, Jiang Qian, Deepak P Edward
We aimed to investigate the proteome changes in anatomical regions of sclera during growth and development of the rabbit. Sclera from New Zealand white rabbits of three ages (1 month, 2 months and 6 months) was dissected into three segments - anterior, equatorial, and posterior. A total of 36 samples were divided into groups by age and anatomical region. Tryptic digests of total proteins were analyzed by liquid chromatography followed by tandem mass spectrometry (LC-MS/MS). Label-free quantification based on spectral counts was used to determine the relative protein abundance and identify proteins with statistically significant differences between age groups or anatomical regions of the sclera...
November 5, 2016: Experimental Eye Research
https://www.readbyqxmd.com/read/27808427/integrin-%C3%AE-v-%C3%AE-8-adopts-a-high-affinity-state-for-soluble-ligands-under-physiological-conditions
#9
Ping Hu, Bing-Hao Luo
It has been proposed that integrins adopt a low affinity conformation under physiological conditions. Integrin can either be activated through cytoplasm or by binding of cations such as Mn(2+) to the head domain. The cytoplasmic activation pathway, i.e. inside-out signaling has been regarded as the physiological pathway for integrin activation. Integrin β8 is important for neuron vascular development. However, due to the highly divergent cytoplasmic domain, this integrin probably does not rely on inside-out signaling for affinity regulation...
November 3, 2016: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/27802203/diagnostic-and-prognostic-roles-of-serum-vitronectin-in-hepatitis-b-related-hepatocellular-carcinoma
#10
Xiao-Ping Yang, Li-Xing Zhou, Qi-Jun Yang, Ling Liu, Yang Cai, Sheng-Lin Ma
BACKGROUND: Vitronectin (VN) might be involved in the progression of hepatocellular carcinoma (HCC). OBJECTIVE: This study was designed to evaluate the diagnostic and prognostic value of serum vitronectin among HCC patients. METHODS: A total of 105 patients with HCC, 91 with liver cirrhosis, 102 with chronic hepatitis, and 100 healthy subjects were recruited. Serum VN and alpha-fetoprotein (AFP) levels were measured. RESULTS: Serum VN levels were significantly higher in HCC patients than in the other groups...
September 26, 2016: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/27798934/vitronectin-binds-to-a-specific-stretch-within-the-head-region-of-yersinia-adhesin-a-and-thereby-modulates-yersinia-enterocolitica-host-interaction
#11
Melanie C Mühlenkamp, Teresia Hallström, Ingo B Autenrieth, Erwin Bohn, Dirk Linke, Janina Rinker, Kristian Riesbeck, Birendra Singh, Jack C Leo, Sven Hammerschmidt, Peter F Zipfel, Monika S Schütz
Complement resistance is an important virulence trait of Yersinia enterocolitica (Ye). The predominant virulence factor expressed by Ye is Yersinia adhesin A (YadA), which enables bacterial attachment to host cells and extracellular matrix and additionally allows the acquisition of soluble serum factors. The serum glycoprotein vitronectin (Vn) acts as an inhibitory regulator of the terminal complement complex by inhibiting the lytic pore formation. Here, we show YadA-mediated direct interaction of Ye with Vn and investigated the role of this Vn binding during mouse infection in vivo...
November 1, 2016: Journal of Innate Immunity
https://www.readbyqxmd.com/read/27795698/implementation-and-evaluation-of-amyloidosis-subtyping-by-laser-capture-microdissection-and-tandem-mass-spectrometry
#12
Peter Mollee, Samuel Boros, Dorothy Loo, Jayde E Ruelcke, Vanessa A Lakis, Kim-Anh Lê Cao, Patricia Renaut, Michelle M Hill
BACKGROUND: Correct identification of the amyloidosis-causing protein is crucial for clinical management. Recently the Mayo Clinic reported laser-capture microdissection (LCM) with liquid chromatography-coupled tandem mass spectrometry (MS/MS) as a new diagnostic tool for amyloid diagnosis. Here, we report an independent implementation of this proteomic diagnostics method at the Princess Alexandra Hospital Amyloidosis Centre in Brisbane, Australia. RESULTS: From 2010 to 2014, 138 biopsies received from 35 different organ sites were analysed by LCM-MS/MS using Congo Red staining to visualise amyloid deposits...
2016: Clinical Proteomics
https://www.readbyqxmd.com/read/27767251/integrin-targeting-and-toxicological-assessment-of-peptide-conjugated-liposome-delivery-systems-to-activated-endothelial-cells
#13
Ali Kermanizadeh, Klaus Villadsen, Ragnhild G Østrem, Knud J Jensen, Peter Møller, Steffen Loft
Utilisation of functionalized liposomes as the means of targeted delivery of therapeutics may enhance specific transport of biologically active drugs to target tissues, while avoiding or reducing undesired side effects. In the present investigation, peptide-conjugated cationic liposomes were constructed with the aim of targeting integrins (i.e. vitronectin and/or fibronectin receptors) on activated endothelial cells. The peptide-conjugated liposomes induced only cytotoxicity at the highest concentration in non-activated or activated endothelial cells, as well as in co-culture of endothelial cells and macrophages...
October 21, 2016: Basic & Clinical Pharmacology & Toxicology
https://www.readbyqxmd.com/read/27725773/analysis-of-the-brain-mural-cell-transcriptome
#14
Liqun He, Michael Vanlandewijck, Elisabeth Raschperger, Maarja Andaloussi Mäe, Bongnam Jung, Thibaud Lebouvier, Koji Ando, Jennifer Hofmann, Annika Keller, Christer Betsholtz
Pericytes, the mural cells of blood microvessels, regulate microvascular development and function and have been implicated in many brain diseases. However, due to a paucity of defining markers, pericyte identification and functional characterization remain ambiguous and data interpretation problematic. In mice carrying two transgenic reporters, Pdgfrb-eGFP and NG2-DsRed, we found that double-positive cells were vascular mural cells, while the single reporters marked additional, but non-overlapping, neuroglial cells...
October 11, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27716559/patients-with-calcific-aortic-stenosis-exhibit-systemic-molecular-evidence-of-ischemia-enhanced-coagulation-oxidative-stress-and-impaired-cholesterol-transport
#15
Laura Mourino-Alvarez, Montserrat Baldan-Martin, Laura Gonzalez-Calero, Carlos Martinez-Laborde, Tamara Sastre-Oliva, Rafael Moreno-Luna, Luis F Lopez-Almodovar, Pedro L Sanchez, Francisco Fernandez-Aviles, Fernando Vivanco, Luis R Padial, Finn Akerstrom, Gloria Alvarez-Llamas, Fernando de la Cuesta, María G Barderas
BACKGROUND: The most common valve diseases are calcific aortic stenosis (AS) and aortic regurgitation (AR). The former is characterized by thickening of valve leaflets followed by progressive calcification, which produces progressive aortic valve (AV) narrowing, increased pressure afterload on the left ventricle (LV) and subsequent LV hypertrophy. On the other hand, AR is due to malcoaptation of the valve leaflets with resultant diastolic reflux of blood from aorta back to the LV producing volume and pressure overload and progressive LV dilatation...
September 26, 2016: International Journal of Cardiology
https://www.readbyqxmd.com/read/27713865/characterization-of-the-adherence-of-clostridium-difficile-spores-the-integrity-of-the-outermost-layer-affects-adherence-properties-of-spores-of-the-epidemic-strain-r20291-to-components-of-the-intestinal-mucosa
#16
Paola Mora-Uribe, Camila Miranda-Cárdenas, Pablo Castro-Córdova, Fernando Gil, Iván Calderón, Juan A Fuentes, Paula I Rodas, Saeed Banawas, Mahfuzur R Sarker, Daniel Paredes-Sabja
Clostridium difficile is the causative agent of the most frequently reported nosocomial diarrhea worldwide. The high incidence of recurrent infection is the main clinical challenge of C. difficile infections (CDI). Formation of C. difficile spores of the epidemic strain R20291 has been shown to be essential for recurrent infection and transmission of the disease in a mouse model. However, the underlying mechanisms of how these spores persist in the colonic environment remains unclear. In this work, we characterized the adherence properties of epidemic R20291 spores to components of the intestinal mucosa, and we assessed the role of the exosporium integrity in the adherence properties by using cdeC mutant spores with a defective exosporium layer...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/27709569/crispr-cas9-mediated-mutagenesis-of-human-pluripotent-stem-cells-in-defined-xeno-free-e8-medium
#17
Chew-Li Soh, Danwei Huangfu
The recent advent of engineered nucleases including the CRISPR/Cas9 system has greatly facilitated genome manipulation in human pluripotent stem cells (hPSCs). In addition to facilitating hPSC-based disease studies, the application of genome engineering in hPSCs has also opened up new avenues for cell replacement therapy. To improve consistency and reproducibility of hPSC-based studies, and to meet the safety and regulatory requirements for clinical translation, it is necessary to use a defined, xeno-free cell culture system...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27678055/persistence-of-fan-shaped-keratocytes-is-a-matrix-rigidity-dependent-mechanism-that-requires-%C3%AE-5%C3%AE-1-integrin-engagement
#18
Maryam Riaz, Marie Versaevel, Danahe Mohammed, Karine Glinel, Sylvain Gabriele
Despite the importance of matrix rigidity on cell functions, many aspects of the mechanosensing process in highly migratory cells remain elusive. Here, we studied the migration of highly motile keratocytes on culture substrates with similar biochemical properties and rigidities spanning the range between soft tissues (~kPa) and stiff culture substrates (~GPa). We show that morphology, polarization and persistence of motile keratocytes are regulated by the matrix stiffness over seven orders of magnitude, without changing the cell spreading area...
September 28, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27659097/pharmacological-targeting-of-plasminogen-activator-inhibitor-1-decreases-vascular-smooth-muscle-cell-migration-and-neointima-formation
#19
Yan Ji, Zhen Weng, Philip Fish, Neha Goyal, Mao Luo, Samantha P Myears, Tammy L Strawn, Bysani Chandrasekar, Jianbo Wu, William P Fay
OBJECTIVE: Plasminogen activator inhibitor-1 (PAI-1), a serine protease inhibitor that promotes and inhibits cell migration, plays a complex and important role in adverse vascular remodeling. Little is known about the effects of pharmacological PAI-1 inhibitors, an emerging drug class, on migration of vascular smooth muscle cells (SMCs) and endothelial cells (ECs), crucial mediators of vascular remodeling. We investigated the effects of PAI-039 (tiplaxtinin), a specific PAI-1 inhibitor, on SMC and EC migration in vitro and vascular remodeling in vivo...
November 2016: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/27650166/intra-and-extracellular-plasminogen-activator-inhibitor-1-regulate-effect-of-vitronectin-against-radiation-induced-endothelial-cell-death
#20
Masaharu Hazawa, Takeshi Yasuda, Ai Saotome-Nakamura, Kenichi Tomiyama, Chizuka Obara, Takaya Goto, Katsushi Tajima
Plasminogen activator inhibitor-1 (PAI-1) is induced by radiation resulting in endothelial cell impairment, potentially leading to multiple organ failure. Vitronectin (VN) is a 75-kDa glycoprotein (VN75) cleaved into two forms (VN75 or VN65/10) by furin, which is regulated by intracellular PAI-1. VN protects against radiation-induced endothelial cell death, but the mechanisms involved in VN processing and its interactions with intra- and extracellular PAI-1 remain unclear. We examined these processes in cells in vitro using recombinant proteins or overexpression of VN and PAI-1 genes, including furin-susceptible (T(381)) and furin-resistant VN (A(381))...
September 17, 2016: Vascular Pharmacology
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