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Vitronectin

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https://www.readbyqxmd.com/read/28209711/serotype-3-pneumococci-sequester-platelet-derived-human-thrombospondin-1-via-the-adhesin-and-immune-evasion-protein-hic
#1
Ulrike Binsker, Thomas P Kohler, Krystin Krauel, Sylvia Kohler, Johanna Habermeyer, Hansjörg Schwertz, Sven Hammerschmidt
Streptococcus pneumoniae serotype 3 strains emerge frequently within clinical isolates of invasive diseases. Bacterial invasion into deeper tissues is associated with colonization and immune evasion mechanisms. Thus, pneumococci express a versatile repertoire of surface proteins sequestering and interacting specifically with components of the human extracellular matrix and serum. Hic, a PspC-like pneumococcal surface protein possesses vitronectin and Factor H binding activity. Here, we show that heterologously expressed Hic domains interact, similar to the classical PspC molecule, with human matricellular thrombospondin-1 (hTSP-1)...
February 16, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28191759/heparin-promotes-cardiac-differentiation-of-human-pluripotent-stem-cells-in-chemically-defined-albumin-free-medium-enabling-consistent-manufacture-of-cardiomyocytes
#2
Yongshun Lin, Kaari L Linask, Barbara Mallon, Kory Johnson, Michael Klein, Jeanette Beers, Wen Xie, Yubin Du, Chengyu Liu, Yinzhi Lai, Jizhong Zou, Mark Haigney, Hushan Yang, Mahendra Rao, Guokai Chen
Cardiomyocytes can be differentiated from human pluripotent stem cells (hPSCs) in defined conditions, but efficient and consistent cardiomyocyte differentiation often requires expensive reagents such as B27 supplement or recombinant albumin. Using a chemically defined albumin-free (E8 basal) medium, we identified heparin as a novel factor that significantly promotes cardiomyocyte differentiation efficiency, and developed an efficient method to differentiate hPSCs into cardiomyocytes. The treatment with heparin helped cardiomyocyte differentiation consistently reach at least 80% purity (up to 95%) from more than 10 different hPSC lines in chemically defined Dulbecco's modified Eagle's medium/F-12-based medium on either Matrigel or defined matrices like vitronectin and Synthemax...
February 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28190785/crosstalk-between-insulin-like-growth-factor-igf-receptor-and-integrins-through-direct-integrin-binding-to-igf1
#3
Yoshikazu Takada, Yoko K Takada, Masaaki Fujita
It has been generally accepted that integrin cell adhesion receptors are involved in growth factor signaling (integrin-growth factor crosstalk), since antagonists to integrins often suppress growth factor signaling. Partly because integrins have been originally identified as cell adhesion receptors to extracellular matrix (ECM) proteins, current models of the crosstalk between IGF1 and integrins propose that ECM ligands (e.g., vitronectin) bind to integrins and IGF1 binds to IGF receptor type 1 (IGF1R), and two separate signals merge inside the cells...
February 3, 2017: Cytokine & Growth Factor Reviews
https://www.readbyqxmd.com/read/28186697/complement-activated-vitronectin-enhances-the-invasion-of-non-phagocytic-cells-by-bacterial-pathogens-burkholderia-and-klebsiella
#4
Yi Han Tan, Akshamal Gamage, Yunn-Hwen Gan
Burkholderia pseudomallei is a serum-resistant Gram-negative bacterium capable of causing disseminated infections with metastatic complications. However, their interaction with non-phagocytic cells is poorly understood. We observed that exposure of B. pseudomallei and the closely related yet avirulent B. thailandensis to human plasma increased epithelial cell invasion by >20 fold. Enhanced invasion was primarily driven by a plasma factor which required a functional complement cascade, but surprisingly, was downstream of C3 mediated opsonisation...
February 10, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28183283/comparative-proteomics-analysis-of-teleost-intermuscular-bones-and-ribs-provides-insight-into-their-development
#5
Chun-Hong Nie, Shi-Ming Wan, Tea Tomljanovic, Tomislav Treer, Chung-Der Hsiao, Wei-Min Wang, Ze-Xia Gao
BACKGROUND: Intermuscular bones (IBs) and ribs both are a part of skeletal system in teleosts, but with different developing process. The chemical composition of fish IBs and ribs as well as the underlying mechanism about their development have not been investigated. In the present study, histological structures showed that one bone cavity containing osteoclasts were existed in ribs, but not in IBs of Megalobrama amblycephala. We constructed the first proteomics map for fish bones including IBs and ribs, and identified the differentially expressed proteins between IBs and ribs through iTRAQ LC-MS/MS proteomic analysis...
February 10, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28150868/identification-of-second-arginine-glycine-aspartic-acid-motif-of-ovine-vitronectin-as-the-complement-c9-binding-site-and-its-implication-in-bacterial-infection
#6
Prasada Rao T, Lakshmi Prasanth T, Parvathy R, Murugavel S, Karuna Devi, Paritosh Joshi
Vitronectin (Vn), a multifunctional protein of blood and extracellular matrix interacts with complement C9. This interaction may modulate innate immunity. Details of Vn-C9 interaction are limited. An assessment of Vn-C9 interaction was made employing goat homologous system. Vn binding to C9 was observed in three different assays. Using recombinant fragments, the C9 binding was mapped to the N-terminus of Vn. Site directed mutagenesis was performed to alter the second RGD sequence (RGD-2) of Vn. Change of R to G or D to A in RGD-2 caused significant decrease in Vn binding to C9 whereas change of R to G in the first RGD motif (RGD-1) had no effect on Vn binding to C9...
February 2, 2017: Microbiology and Immunology
https://www.readbyqxmd.com/read/28143632/extracellular-like-matrices-and-leukaemia-inhibitory-factor-for-in-vitro-culture-of-human-primordial-follicles
#7
Assiel J Younis, Galit Lerer-Serfaty, Dana Stav, Bethsabee Sabbah, Tzippy Shochat, Gania Kessler-Icekson, Muayad A Zahalka, Michal Shachar-Goldenberg, Avi Ben-Haroush, Benjamin Fisch, Ronit Abir
The possibility of maturing human primordial follicles in vitro would assist fertility restoration without the danger of reseeding malignancies. Leukaemia inhibitory factor (LIF) and certain culture matrices may promote human follicular growth. The present study compared human primordial follicular growth on novel culture matrices, namely human recombinant vitronectin (hrVit), small intestine submucosa (SIS), alginate scaffolds and human recombinant virgin collagen bioengineered in tobacco plant lines (CollPlant)...
February 1, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28134277/efficient-adhesion-culture-of-human-pluripotent-stem-cells-using-laminin-fragments-in-an-uncoated-manner
#8
Takamichi Miyazaki, Takehisa Isobe, Norio Nakatsuji, Hirofumi Suemori
We describe highly effective adhesion culture of human pluripotent stem cells (hPSCs) using laminin fragments without precoating. Culture substrates have been generally thought to exert a cell adhesion effect when they are precoated onto culture vessels. However, simple addition of laminin fragments to a cell suspension during passaging accelerated the adhesion of single dissociated hPSCs onto culture vessels that were not precoated with any culture substrate. Interestingly, similar to conventional precoating, the uncoated addition of laminin fragments supported robust adhesion of single hPSCs and maximum adhesion at a much lower concentration compared with precoating...
January 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28133907/discovery-of-gastric-cancer-specific-biomarkers-by-the-application-of-serum-proteomics
#9
Moon-Won Yoo, Jisook Park, Hye-Seung Han, Yeo-Min Yun, Jeong Won Kang, Do-Young Choi, Joon Won Lee, Jae Hun Jung, Kyung-Yung Lee, Kwang Pyo Kim
Current diagnostic markers for gastric cancer are not sufficiently specific or sensitive for use in clinical practice. The aims of this study are to compare the proteomes of serum samples from patients with gastric cancers and normal controls, and to develop useful tumor markers of gastric cancer by quantitative proteomic analysis. We identified a total of 388 proteins with a ≤1% false discovery rate and with at least two unique peptides from the sera of each group. Among them, 215, 251, and 260 proteins were identified in serum samples of patients in an advanced cancer group, early cancer group, and normal control group, respectively...
January 29, 2017: Proteomics
https://www.readbyqxmd.com/read/28128374/the-influence-of-surface-modified-poly-l-lactic-acid-films-on-the-differentiation-of-human-monocytes-into-macrophages
#10
Clara R Correia, Joana Gaifem, Mariana B Oliveira, Ricardo Silvestre, João F Mano
Macrophages play a crucial role in the biological performance of biomaterials, as key factors in defining the optimal inflammation-healing balance towards tissue regeneration and implant integration. Here, we investigate how different surface modifications performed on poly(l-lactic acid) (PLLA) films would influence the differentiation of human monocytes into macrophages. We tested PLLA films without modification, surface-modified by plasma treatment (pPLLA) or by combining plasma treatment with different coating materials, namely poly(l-lysine) and a series of proteins from the extracellular matrix: collagen I, fibronectin, vitronectin, laminin and albumin...
January 27, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28121998/rheumatic-heart-disease-and-myxomatous-degeneration-differences-and-similarities-of-valve-damage-resulting-from-autoimmune-reactions-and-matrix-disorganization
#11
Carlo de Oliveira Martins, Lea Demarchi, Frederico Moraes Ferreira, Pablo Maria Alberto Pomerantzeff, Carlos Brandao, Roney Orismar Sampaio, Guilherme Sobreira Spina, Jorge Kalil, Edecio Cunha-Neto, Luiza Guilherme
Autoimmune inflammatory reactions leading to rheumatic fever (RF) and rheumatic heart disease (RHD) result from untreated Streptococcus pyogenes throat infections in individuals who exhibit genetic susceptibility. Immune effector mechanisms have been described that lead to heart tissue damage culminating in mitral and aortic valve dysfunctions. In myxomatous valve degeneration (MXD), the mitral valve is also damaged due to non-inflammatory mechanisms. Both diseases are characterized by structural valve disarray and a previous proteomic analysis of them has disclosed a distinct profile of matrix/structural proteins differentially expressed...
2017: PloS One
https://www.readbyqxmd.com/read/28086938/alpha-enolase-eno1-controls-alpha-v-beta-3-integrin-expression-and-regulates-pancreatic-cancer-adhesion-invasion-and-metastasis
#12
Moitza Principe, Simone Borgoni, Mariafrancesca Cascione, Michelle Samuel Chattaragada, Sammy Ferri-Borgogno, Michela Capello, Sara Bulfamante, Jennifer Chapelle, Francesca Di Modugno, Paola Defilippi, Paola Nisticò, Paola Cappello, Chiara Riganti, Stefano Leporatti, Francesco Novelli
BACKGROUND: We have previously shown that in pancreatic ductal adenocarcinoma (PDA) cells, the glycolytic enzyme alpha-enolase (ENO1) also acts as a plasminogen receptor and promotes invasion and metastasis formation. Moreover, ENO1 silencing in PDA cells induces oxidative stress, senescence and profoundly modifies PDA cell metabolism. Although anti-ENO1 antibody inhibits PDA cell migration and invasion, little is known about the role of ENO1 in regulating cell-cell and cell-matrix contacts...
January 13, 2017: Journal of Hematology & Oncology
https://www.readbyqxmd.com/read/28068081/peptide-decorated-nanofibrous-niche-augments-in-vitro-directed-osteogenic-conversion-of-human-pluripotent-stem-cells
#13
Yi Deng, Yuanyi Yang, Shicheng Wei
Realization of clinical potential of human pluripotent stem cells (hPSCs) in bone regenerative medicine requires development of simple and safe biomaterials for expansion of hPSCs followed by directing their lineage commitment to osteoblasts. In the present study, a chemically defined peptide-decorated polycaprolactone (PCL) nanofibrous microenvironment was prepared through electrospinning technology and subsequent conjugation with vitronectin peptide to promote the culture and osteogenic potential of hPSCs in vitro...
January 24, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28053625/lipid-raft-integrity-is-required-for-survival-of-triple-negative-breast-cancer-cells
#14
Anil Badana, Madhuri Chintala, Gayathri Varikuti, Nagaseshu Pudi, Seema Kumari, Vijaya Rachel Kappala, Rama Rao Malla
PURPOSE: Lipid rafts are cholesterol enriched microdomains that colocalize signaling pathways involved in cell proliferation, metastasis, and angiogenesis. We examined the effect of methyl-β-cyclodextrin (MβCD)-mediated cholesterol extraction on the proliferation, adhesion, invasion, and angiogenesis of triple negative breast cancer (TNBC) cells. METHODS: We measured cholesterol and estimated cell toxicity. Detergent resistant membrane (DRM) and non-DRM fractions were separated using the OptiPrep gradient method...
December 2016: Journal of Breast Cancer
https://www.readbyqxmd.com/read/28017869/endosteal-like-extracellular-matrix-expression-on-melt-electrospun-written-scaffolds
#15
Maria Lourdes Muerza-Cascante, Ali Shokoohmand, Kiarash Khosrotehrani, David Haylock, Paul D Dalton, Dietmar W Hutmacher, Daniela Loessner
: Tissue engineering technology platforms constitute a unique opportunity to integrate cells and extracellular matrix (ECM) proteins into scaffolds and matrices that mimic the natural microenvironment in vitro. The development of tissue-engineered 3D models that mimic the endosteal microenvironment enables researchers to discover the causes and improve treatments for blood and immune-related diseases. The aim of this study was to establish a physiologically relevant in vitro model using 3D printed scaffolds to assess the contribution of human cells to the formation of a construct that mimics human endosteum...
December 22, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/28009413/role-of-dextran-in-maintaining-adhesive-and-stiffness-properties-of-prestripped-dmek-lenticules
#16
Mohit Parekh, Alessandro Ruzza, Rosa Di Mundo, Stefano Ferrari, Giuseppina Recchia, Hossein Elbadawy, Giuseppe Carbone, Diego Ponzin
PURPOSE: To investigate the adhesive and stiffness properties of prestripped Descemet membrane endothelial keratoplasty (DMEK) lenticules in different preservation conditions (with and without dextran). METHODS: The study included 3 conditions: (C1) tissues collected from tissue culture media (TCM), stripped and preserved in TCM; (C2) tissues collected from transport media (TM) (TCM supplemented with 6% dextran T-500), stripped and preserved in TM; and (C3) tissues collected from TCM, stripped and preserved in TM...
November 25, 2016: European Journal of Ophthalmology
https://www.readbyqxmd.com/read/27998942/integrins-functioning-in-uterine-endometrial-stromal-and-epithelial-cells-in-estrus
#17
Hye Jin Park, Ji Eun Park, Hyun Lee, Seong Jae Kim, Jung Im Yun, Minseok Kim, Kyu Hyun Park, Seung Tae Lee
Here, as a basic study in the construction of a non-cellular niche that supports artificial organization of three-dimensional endometrial tissue, we defined the types of integrin heterodimers that are expressed transcriptionally, translationally and functionally in endometrial stromal (ES) and endometrial epithelial (EE) cells isolated from the mouse uterus in estrus. Gene and protein expression of integrin subunits were analyzed at the transcriptional and translational level by real-time PCR and fluorescent immunoassay, respectively...
March 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/27989923/a-robust-vitronectin-derived-peptide-for-the-scalable-long-term-expansion-and-neuronal-differentiation-of-human-pluripotent-stem-cell-hpsc-derived-neural-progenitor-cells-hnpcs
#18
Divya Varun, Gayathri Rajaram Srinivasan, Yi-Huan Tsai, Hyun-Je Kim, Joshua Cutts, Francis Petty, Ryan Merkley, Nicholas Stephanopoulos, Dasa Dolezalova, Martin Marsala, David A Brafman
Despite therapeutic advances, neurodegenerative diseases and disorders remain some of the leading causes of mortality and morbidity in the United States. Therefore, cell-based therapies to replace lost or damaged neurons and supporting cells of the central nervous system (CNS) are of great therapeutic interest. To that end, human pluripotent stem cell (hPSC) derived neural progenitor cells (hNPCs) and their neuronal derivatives could provide the cellular 'raw material' needed for regenerative medicine therapies for a variety of CNS disorders...
January 15, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/27989763/the-multifaceted-roles-of-leptospira-enolase
#19
Natália Salazar, Matilde Costa Lima de Souza, Amanda Gameiro Biasioli, Ludmila Bezerra da Silva, Angela Silva Barbosa
A previous study had demonstrated that Leptospira enolase is secreted extracellularly by a yet unknown mechanism and reassociates with the bacterial membrane. Surface-anchored leptospiral enolase displays plasminogen binding activity. In this work, we explored the consequences of this interaction and also assessed whether Leptospira enolase might display additional moonlighting functions by interacting with other host effector proteins. We first demonstrated that enolase-bound plasminogen is converted to its active form, plasmin...
October 28, 2016: Research in Microbiology
https://www.readbyqxmd.com/read/27978828/integrin-fak-signaling-rapidly-and-potently-promotes-mitochondrial-function-through-stat3
#20
Nishant P Visavadiya, Matthew P Keasey, Vladislav Razskazovskiy, Kalpita Banerjee, Cuihong Jia, Chiharu Lovins, Gary L Wright, Theo Hagg
BACKGROUND: STAT3 is increasingly becoming known for its non-transcriptional regulation of mitochondrial bioenergetic function upon activation of its S727 residue (S727-STAT3). Lengthy mitochondrial dysfunction can lead to cell death. We tested whether an integrin-FAK-STAT3 signaling pathway we recently discovered regulates mitochondrial function and cell survival, and treatments thereof. METHODS: Cultured mouse brain bEnd5 endothelial cells were treated with integrin, FAK or STAT3 inhibitors, FAK siRNA, as well as integrin and STAT3 activators...
15, 2016: Cell Communication and Signaling: CCS
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