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Vitronectin

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https://www.readbyqxmd.com/read/28640945/production-of-human-vitronectin-in-nicotiana-benthamiana-using-the-inpact-hyper-expression-platform
#1
Benjamin Dugdale, Maiko Kato, Pradeep Deo, Manuel Plan, Mark Harrison, Robyn Lloyd, Terry Walsh, Robert Harding, James Dale
Human vitronectin (hVN) is a glycoprotein that functions as a cell adhesion molecule and a regulator of coagulation in blood plasma and the extracellular matrix. In vitro, hVN is added to serum-free media in order to promote the adhesion of animal cells to tissue culture surfaces and the proliferation of undifferentiated stem cells. Here, we report the production of hVN in Nicotiana benthamiana using the inducible In Plant Activation (INPACT) hyper-expression platform. N. benthamiana plants were transformed with an INPACT expression cassette encoding hVN, and both the Tobacco yellow dwarf virus Rep/RepA activator and Tomato bushy stunt virus p19 gene under the transcriptional control of the ethanol-inducible AlcR:alcA gene switch...
June 22, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28621746/biocompatibility-analyses-of-al%C3%A2-o%C3%A2-treated-titanium-plates-tested-with-osteocyte-and-fibroblast-cell-lines
#2
Alberto Smargiassi, Jessika Bertacchini, Marta Checchi, Francesco Cavani, Marzia Ferretti, Carla Palumbo
Osseointegration of a titanium implant is still an issue in dental/orthopedic implants durable over time. The good integration of these implants is mainly due to their surface and topography. We obtained an innovative titanium surface by shooting different-in-size particles of Al₂O₃ against the titanium scaffolds which seems to be ideal for bone integration. To corroborate that, we used two different cell lines: MLO-Y4 (murine osteocytes) and 293 (human fibroblasts) and tested the titanium scaffolds untreated and treated (i...
June 16, 2017: Biomedicines
https://www.readbyqxmd.com/read/28580316/interactome-mapping-guided-by-tissue-specific-phosphorylation-in-age-related-macular-degeneration
#3
Srinivas R Sripathi, Weilue He, Cameron L Prigge, O'Donnell Sylvester, Ji-Yeon Um, Folami L Powell, Musa Neksumi, Paul S Bernstein, Dong-Won Choo, Manuela Bartoli, Diana R Gutsaeva, Wan Jin Jahng
The current study aims to determine the molecular mechanisms of age-related macular degeneration (AMD) using the phosphorylation network. Specifically, we examined novel biomarkers for oxidative stress by protein interaction mapping using in vitro and in vivo models that mimic the complex and progressive characteristics of AMD. We hypothesized that the early apoptotic reactions could be initiated by protein phosphorylation in region-dependent (peripheral retina vs. macular) and tissue-dependent (retinal pigment epithelium vs...
February 2017: International Journal of Scientific and Engineering Research
https://www.readbyqxmd.com/read/28575979/live-cell-biosensor-for-assessment-of-adhesion-qualities-of-biomaterials
#4
Svetlana I Ivanova, Stoyan Chakarov, Albena Momchilova, Roumen Pankov
The present study describes a live-cell biosensor, suitable for general evaluation of adhesion qualities of different substrates. It is based on NIH/3T3 fibroblast cell line stably expressing fusion fluorescently tagged proteins mCherry-vinculin and GFP-tensin as quantifiable markers for assessment not only of focal but also of fibrillar contacts. Four measurable parameters - spreading, polarization and development of focal and fibrillar adhesions were used to standardize the adhesion of biosensor cells after plating on five substrates of natural origin - fibronectin, vitronectin, laminin-111, laminin-521 and collagen type I...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28567614/a-synonymous-mutation-at-bovine-alpha-vitronectin-domain-of-integrin-host-receptor-itgav-gene-effect-the-susceptibility-of-foot-and-mouth-disease-in-crossbred-cattle
#5
Rani Singh, Rani Alex, Umesh Singh, Sushil Kumar, Gyanendra Singh Sengar, T V Raja, R R Alyethodi, Ashish Kumar, Rajib Deb
Integrins are one of the major biologically active proteins responsible for Foot-and-mouth disease virus (FMDV)- host interaction. Out of various heterodimeric integrins discovered, αVβ6 heterodimer serves as the chief receptor for FMDV host tropism. Earlier studies reported that, SNPs at beta 6 subunit (ITGB6) were associated with the occurrence of the diseases in cattle. In this study we report the association between a synonymous SNP (rs719257875) at bovine alpha vitronectin domain of integrin receptor (ITGAV) gene and FMD susceptibility in cattle...
June 1, 2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28551079/adhesion-attenuates-respiratory-burst-induced-by-different-modes-of-triggering-in-resting-or-lps-primed-neutrophils
#6
Terukazu Sanui, Masaaki Takeshita, Takao Fukuda, Urara Tanaka, Rehab Alshargabi, Yoshitomi Aida, Fusanori Nishimura
The effects of adherence on neutrophil superoxide anion (O2(-)) generation triggered by surface, soluble ligand, or adherence were studied. Resting-neutrophils adhered to the uncoated tubes resulting in O2(-) generation, but not on plasma-, fibrinogen-, vitronectin-, fibronectin-, laminin-, collagen-, or poly HEMA-coated surfaces. Enhanced N-formyl-methionyl-leucyl-phenylalanine (fMLP)-stimulated O2(-) generation by LPS-primed-neutrophils was induced by the incubation on plasma, fibrinogen, vitronectin, fibronectin, or laminin in the absence of Mg(2+)...
August 2017: Immunobiology
https://www.readbyqxmd.com/read/28537496/feeder-free-derivation-of-na%C3%A3-ve-human-pluripotent-stem-cells
#7
Emily Ward, Kirk Twaroski, Jakub Tolar
Human pluripotent stem cells (HPSCs) cultured in conditions that maintain pluripotency via FGF and TGFβ signaling have been described as being in a primed state. These cells have been shown to exhibit characteristics more closely related to mouse epiblast-derived stem cells than to so called naïve mouse PSCs said to possess a more ground state pluripotency that mimics the early mouse embryo inner cell mass. Initial attempts to create culture conditions favorable for generation of naïve HPSCs from primed HPSCs has required the use of mouse embryonic fibroblasts as a feeder layer to support this transition...
May 24, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28501006/liquid-chromatography-mass-spectrometry-based-quantitative-proteomics-analysis-reveals-chondroprotective-effects-of-astragaloside-iv-in-interleukin-1%C3%AE-induced-sw1353-chondrocyte-like-cells
#8
Huali Luo, Ling Yao, Yudi Zhang, Rongheng Li
OBJECTIVE: Chondrocyte apoptosis played a key role on the progression of Osteoarthritis (OA). Safe and effective drugs are urgently needed for the treatment of OA. Previous study reported that Astragaloside IV (ASG-IV) had exerted a protective effect against articular cartilage degeneration by promoting rapid proliferation of chondrocyte. Therefore, the aim of our study is to explore the effects and mechanisms of ASG-IV in chondrocyte apoptosis. METHODS: Isobaric Tags For Relative And Absolute Quantitation (iTRAQ)-based quantitative proteomics was used to quantitatively detect and map proteins in SW1353 chondrocyte-like cells pre-treated with ASG-IV or interleukin-1β (IL-1β) or ASG-IV+IL-1β...
July 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28473293/moesin-and-merlin-regulate-urokinase-receptor-dependent-endothelial-cell-migration-adhesion-and-angiogenesis
#9
Bernard Degryse, Mishan Britto, Chun Xu Shan, Robert G Wallace, Keith D Rochfort, Philip M Cummins, Gerardene Meade, Ronan P Murphy
The glycosyl-phosphatidyl-inositol (GPI)-anchored urokinase receptor (uPAR) has no intracellular domain, but nevertheless initiates signalling through proximal interactions with other membrane receptors including integrins. The relationships between uPAR and ezrin/radixin/moesin (ERM) proteins, moesin and merlin have never been explored. Moesin and merlin are versatile membrane-actin links and regulators of receptors signalling, respectively. We show that uPAR controls moesin and merlin, which propagate uPAR-initiated signals and modulate integrin functions, thereby regulating uPAR activity...
May 1, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28427432/anti-human-platelet-antigen-hpa-1a-antibodies-may-affect-trophoblast-functions-crucial-for-placental-development-a-laboratory-study-using-an-in-vitro-model
#10
Mariana Eksteen, Gøril Heide, Heidi Tiller, Yan Zhou, Nora Hersoug Nedberg, Inigo Martinez-Zubiaurre, Anne Husebekk, Bjørn R Skogen, Tor B Stuge, Mette Kjær
BACKGROUND: Fetal and neonatal alloimmune thrombocytopenia (FNAIT) is a bleeding disorder caused by maternal antibodies against paternal human platelet antigens (HPAs) on fetal platelets. Antibodies against HPA-1a are accountable for the majority of FNAIT cases. We have previously shown that high levels of maternal anti-HPA-1a antibodies are associated with clinically significant reduced birth weight in newborn boys. Chronic inflammatory placental lesions are associated with increased risk of reduced birth weight and have previously been reported in connection with FNAIT pregnancies...
April 21, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28411808/validation-of-a-new-method-by-nano-liquid-chromatography-on-chip-tandem-mass-spectrometry-for-combined-quantitation-of-c3f-and-the-v65-vitronectin-fragment-as-biomarkers-of-diagnosis-and-severity-of-osteoarthritis
#11
Gaël Cobraiville, Marianne Fillet, Mohammed Sharif, Khadija Ourradi, Gwenaël Nys, Michel G Malaise, Dominique de Seny
Microfluidic liquid chromatography coupled to a nanoelectrospray source ion trap mass spectrometry was used for the absolute and simultaneous quantitation of C3f and the V65 vitronectin fragment in serum. The method was first carefully optimized and then validated in serum biological matrix. Stable isotopes for the two biomarkers of interest were used as stable isotope labeled peptide standards. A weighted 1/x(2) quadratic regression for C3f and a weighted 1/x quadratic regression for the V65 vitronectin peptide were selected for calibration curves...
July 1, 2017: Talanta
https://www.readbyqxmd.com/read/28398652/brucella-abortus-activated-microglia-induce-neuronal-death-through-primary-phagocytosis
#12
Ana M Rodríguez, M Victoria Delpino, M Cruz Miraglia, Miriam M Costa Franco, Paula Barrionuevo, Vida A Dennis, Sergio C Oliveira, Guillermo H Giambartolomei
Inflammation has long been implicated as a contributor to pathogenesis in neurobrucellosis. Many of the associated neurocognitive symptoms of neurobrucellosis may be the result of neuronal dysfunction resulting from the inflammatory response induced by Brucella abortus infection in the central nervous system. In this manuscript, we describe an immune mechanism for inflammatory activation of microglia that leads to neuronal death upon B. abortus infection. B. abortus was unable to infect or harm primary cultures of mouse neurons...
April 11, 2017: Glia
https://www.readbyqxmd.com/read/28377270/expression-and-functional-characterization-of-vitronectin-gene-from-japanese-flounder-paralichthys-olivaceus
#13
Shuo Li, Gaixiang Hao, Weijiao Peng, Xuyun Geng, Jinsheng Sun
Vitronectin (Vtn) is a multifunctional protein that plays significant roles in cell adhesion, migration, spreading and survival, and in the regulation of membrane attack complex formation and the terminal pathway of complement activation in innate immune response. However, the expression and immune significance of Vtn in fish remains largely unknown. In order to understand the involvement of Vtn in fish innate immune response, here we cloned and characterized a full-length Vtn ortholog cDNA, termed PoVtn, from Japanese flounder Paralichthys olivaceus...
April 1, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28336232/tgf%C3%AE-1-induces-endometrial-cancer-cell-adhesion-and-migration-by-up-regulating-integrin-%C3%AE-v%C3%AE-3-via-smad-independent-mek-erk1-2-signaling
#14
Siyuan Xiong, Christian Klausen, Jung-Chien Cheng, Peter C K Leung
Endometrial cancer is the most common, and second most lethal, gynecological malignancy, and its rates of incidence and death are growing. This is likely attributable to increased numbers of high-risk type II endometrial cancers which account for ~30% of cases but ~75% of deaths due to their aggressive and metastatic behaviour. Histopathological and in vitro functional studies suggest that aberrant TGFβ1 signaling may contribute to endometrial cancer development and the acquisition of invasive/metastatic characteristics...
March 21, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28332572/xeno-free-culture-of-human-pluripotent-stem-cells-on-oligopeptide-grafted-hydrogels-with-various-molecular-designs
#15
Yen-Ming Chen, Li-Hua Chen, Meng-Pei Li, Hsing-Fen Li, Akon Higuchi, S Suresh Kumar, Qing-Dong Ling, Abdullah A Alarfaj, Murugan A Munusamy, Yung Chang, Giovanni Benelli, Kadarkarai Murugan, Akihiro Umezawa
Establishing cultures of human embryonic (ES) and induced pluripotent (iPS) stem cells in xeno-free conditions is essential for producing clinical-grade cells. Development of cell culture biomaterials for human ES and iPS cells is critical for this purpose. We designed several structures of oligopeptide-grafted poly (vinyl alcohol-co-itaconic acid) hydrogels with optimal elasticity, and prepared them in formations of single chain, single chain with joint segment, dual chain with joint segment, and branched-type chain...
March 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28315346/inhibition-of-osteoporosis-by-the-%C3%AE-v%C3%AE-3-integrin-antagonist-of-rhodostomin-variants
#16
Tzu-Hung Lin, Rong-Sen Yang, Huang-Ju Tu, Houng-Chi Liou, Yen-Ming Lin, Woie-Jer Chuang, Wen-Mei Fu
Integrins are heterodimeric cell surface receptors that mediate cell-cell and cell-matrix interaction. The vitronectin and osteopontin receptor αvβ3 integrin has increased expression levels and is implicated in the adhesion, activation, and migration of osteoclasts on the bone surface as well as osteoclast polarization. αvβ3 integrin plays an important role in osteoclast differentiation and resorption. In addition, Arg-Gly-Asp (RGD)-containing peptides, small molecular inhibitors, and antibodies to αvβ3 integrin have been shown to inhibit bone resorption in vitro and in vivo...
June 5, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28299286/the-rickettsia-conorii-adr1-interacts-with-the-c-terminus-of-human-vitronectin-in-a-salt-sensitive-manner
#17
Abigail I Fish, Sean P Riley, Birendra Singh, Kristian Riesbeck, Juan J Martinez
Spotted fever group (SFG) Rickettsia species are inoculated into the mammalian bloodstream by hematophagous arthropods. Once in the bloodstream and during dissemination, the survival of these pathogens is dependent upon the ability of these bacteria to evade serum-borne host defenses until a proper cellular host is reached. Rickettsia conorii expresses an outer membrane protein, Adr1, which binds the complement inhibitory protein vitronectin to promote resistance to the anti-bacterial effects of the terminal complement complex...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28279737/targeting-tumors-with-cyclic-rgd-conjugated-lipid-nanoparticles-loaded-with-an-ir780-nir-dye-in-vitro-and-in-vivo-evaluation
#18
Jungyoon Choi, Emilie Rustique, Maxime Henry, Mélanie Guidetti, Véronique Josserand, Lucie Sancey, Jérôme Boutet, Jean-Luc Coll
Like several 50nm-large nanocarriers, lipid nanoparticles (LNPs) can passively accumulate in tumors through the Enhanced Permeability and Retention effect. In this study, we developed PEGylated LNPs loaded with IR780 iodide as a contrast agent for NIR fluorescence imaging and modified them with cyclic RGD peptides in order to target integrin avβ3. We demonstrate a specific targeting of the receptor with cRGD-LNPs but not with cRAD-LNP and standard LNP using HEK293(β3), HEK293(β3)-αvRFP, DU145 and PC3 cell lines...
March 6, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28256735/using-affinity-capillary-electrophoresis-and-computational-models-for-binding-studies-of-heparinoids-with-p-selectin-and-other-proteins
#19
Mona Mozafari, Shantheya Balasupramaniam, Lutz Preu, Sami El Deeb, Christian G Reiter, Hermann Wätzig
A fast and precise affinity capillary electrophoresis (ACE) method has been developed and applied for the investigation of the binding interactions between P-selectin and heparinoids as potential P-selectin inhibitors in the presence and absence of calcium ions. Furthermore, model proteins and vitronectin were used to appraise the binding behavior of P-selectin. The normalized mobility ratios (∆R/Rf ), which provided information about the binding strength and the overall charge of the protein-ligand complex, were used to evaluate the binding affinities...
March 3, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28247073/-proteome-analysis-of-undiluted-vitreous-humor-in-patients-with-branch-retinal-vein-occlusion
#20
I Dacheva, M Reich, M Nobl, K Ceglowska, J Wasiak, J Siwy, P Zürbig, H Mischak, F H J Koch, J Kopitz, F T A Kretz, T Tandogan, G U Auffarth, M J Koss
BACKGROUND: The pathophysiological mechanisms of macular edema secondary to branch retinal vein occlusion (BRVO) remain unclear. OBJECTIVES: To analyze the protein profile of human vitreous of patients with BRVO and to identify specific dysregulated proteins. MATERIALS AND METHODS: Undiluted vitreous humor samples from patients with treatment naïve BRVO and 15 controls with idiopathic floaters were analyzed in this clinical-experimental study using capillary electrophoresis coupled to a mass spectrometer (CE-MS) and tandem mass spectrometry (MS/MS)...
February 28, 2017: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
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