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Vitronectin

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https://www.readbyqxmd.com/read/28086938/alpha-enolase-eno1-controls-alpha-v-beta-3-integrin-expression-and-regulates-pancreatic-cancer-adhesion-invasion-and-metastasis
#1
Moitza Principe, Simone Borgoni, Mariafrancesca Cascione, Michelle Samuel Chattaragada, Sammy Ferri-Borgogno, Michela Capello, Sara Bulfamante, Jennifer Chapelle, Francesca Di Modugno, Paola Defilippi, Paola Nisticò, Paola Cappello, Chiara Riganti, Stefano Leporatti, Francesco Novelli
BACKGROUND: We have previously shown that in pancreatic ductal adenocarcinoma (PDA) cells, the glycolytic enzyme alpha-enolase (ENO1) also acts as a plasminogen receptor and promotes invasion and metastasis formation. Moreover, ENO1 silencing in PDA cells induces oxidative stress, senescence and profoundly modifies PDA cell metabolism. Although anti-ENO1 antibody inhibits PDA cell migration and invasion, little is known about the role of ENO1 in regulating cell-cell and cell-matrix contacts...
January 13, 2017: Journal of Hematology & Oncology
https://www.readbyqxmd.com/read/28068081/peptide-decorated-nanofibrous-niche-augments-in-vitro-directed-osteogenic-conversion-of-human-pluripotent-stem-cells
#2
Yi Deng, Yuanyi Yang, Shicheng Wei
Realization of clinical potential of human pluripotent stem cells (hPSCs) in bone regenerative medicine requires development of simple and safe biomaterials for expansion of hPSCs followed by directing their lineage commitment to osteoblasts. In the present study, a chemically defined peptide-decorated polycaprolactone (PCL) nanofibrous microenvironment was prepared through electrospinning technology and subsequent conjugation with vitronectin peptide to promote the culture and osteogenic potential of hPSCs in vitro...
January 9, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28053625/lipid-raft-integrity-is-required-for-survival-of-triple-negative-breast-cancer-cells
#3
Anil Badana, Madhuri Chintala, Gayathri Varikuti, Nagaseshu Pudi, Seema Kumari, Vijaya Rachel Kappala, Rama Rao Malla
PURPOSE: Lipid rafts are cholesterol enriched microdomains that colocalize signaling pathways involved in cell proliferation, metastasis, and angiogenesis. We examined the effect of methyl-β-cyclodextrin (MβCD)-mediated cholesterol extraction on the proliferation, adhesion, invasion, and angiogenesis of triple negative breast cancer (TNBC) cells. METHODS: We measured cholesterol and estimated cell toxicity. Detergent resistant membrane (DRM) and non-DRM fractions were separated using the OptiPrep gradient method...
December 2016: Journal of Breast Cancer
https://www.readbyqxmd.com/read/28017869/endosteal-like-extracellular-matrix-expression-on-melt-electrospun-written-scaffolds
#4
Maria Lourdes Muerza-Cascante, Ali Shokoohmand, Kiarash Khosrotehrani, David Haylock, Paul D Dalton, Dietmar W Hutmacher, Daniela Loessner
: Tissue engineering technology platforms constitute a unique opportunity to integrate cells and extracellular matrix (ECM) proteins into scaffolds and matrices that mimic the natural microenvironments in vitro. The developments of tissue-engineered 3D models that mimic the endosteal microenvironment enable researchers to discover the causes and improve treatments for blood and immune-related diseases. The aim of this study was to establish a physiologically relevant in vitro model using 3D printed scaffolds to assess the contribution of human cells to the formation of a construct that mimics human endosteum...
December 22, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/28009413/role-of-dextran-in-maintaining-adhesive-and-stiffness-properties-of-prestripped-dmek-lenticules
#5
Mohit Parekh, Alessandro Ruzza, Rosa Di Mundo, Stefano Ferrari, Giuseppina Recchia, Hossein Elbadawy, Giuseppe Carbone, Diego Ponzin
PURPOSE: To investigate the adhesive and stiffness properties of prestripped Descemet membrane endothelial keratoplasty (DMEK) lenticules in different preservation conditions (with and without dextran). METHODS: The study included 3 conditions: (C1) tissues collected from tissue culture media (TCM), stripped and preserved in TCM; (C2) tissues collected from transport media (TM) (TCM supplemented with 6% dextran T-500), stripped and preserved in TM; and (C3) tissues collected from TCM, stripped and preserved in TM...
November 25, 2016: European Journal of Ophthalmology
https://www.readbyqxmd.com/read/27998942/integrins-functioning-in-uterine-endometrial-stromal-and-epithelial-cells-in-estrus
#6
Hye Jin Park, Ji Eun Park, Hyun Lee, Seong Jae Kim, Jung Im Yun, Minseok Kim, Kyu Hyun Park, Seung Tae Lee
Here, as a basic study in the construction of a non-cellular niche that supports artificial organization of three dimensional endometrial tissue, we defined the types of integrin heterodimers that are expressed transcriptionally, translationally, and functionally in endometrial stromal (ES) and endometrial epithelial (EE) cells isolated from the mouse uterus in estrus. Gene and protein expression of integrin subunits were analyzed at the transcriptional and translational level by real-time PCR and fluorescent immunoassay, respectively...
December 20, 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/27989923/a-robust-vitronectin-derived-peptide-for-the-scalable-long-term-expansion-and-neuronal-differentiation-of-human-pluripotent-stem-cell-hpsc-derived-neural-progenitor-cells-hnpcs
#7
Divya Varun, Gayathri Rajaram Srinivasan, Yi-Huan Tsai, Hyun-Je Kim, Joshua Cutts, Francis Petty, Ryan Merkley, Nicholas Stephanopoulos, Dasa Dolezalova, Martin Marsala, David A Brafman
Despite therapeutic advances, neurodegenerative diseases and disorders remain some of the leading causes of mortality and morbidity in the United States. Therefore, cell-based therapies to replace lost or damaged neurons and supporting cells of the central nervous system (CNS) are of great therapeutic interest. To that end, human pluripotent stem cell (hPSC) derived neural progenitor cells (hNPCs) and their neuronal derivatives could provide the cellular 'raw material' needed for regenerative medicine therapies for a variety of CNS disorders...
January 15, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/27989763/the-multifaceted-roles-of-leptospira-enolase
#8
Natália Salazar, Matilde Costa Lima de Souza, Amanda Gameiro Biasioli, Ludmila Bezerra da Silva, Angela Silva Barbosa
A previous study had demonstrated that Leptospira enolase is secreted extracellularly by a yet unknown mechanism and reassociates with the bacterial membrane. Surface-anchored leptospiral enolase displays plasminogen binding activity. In this work, we explored the consequences of this interaction and also assessed whether Leptospira enolase might display additional moonlighting functions by interacting with other host effector proteins. We first demonstrated that enolase-bound plasminogen is converted to its active form, plasmin...
October 28, 2016: Research in Microbiology
https://www.readbyqxmd.com/read/27978828/integrin-fak-signaling-rapidly-and-potently-promotes-mitochondrial-function-through-stat3
#9
Nishant P Visavadiya, Matthew P Keasey, Vladislav Razskazovskiy, Kalpita Banerjee, Cuihong Jia, Chiharu Lovins, Gary L Wright, Theo Hagg
BACKGROUND: STAT3 is increasingly becoming known for its non-transcriptional regulation of mitochondrial bioenergetic function upon activation of its S727 residue (S727-STAT3). Lengthy mitochondrial dysfunction can lead to cell death. We tested whether an integrin-FAK-STAT3 signaling pathway we recently discovered regulates mitochondrial function and cell survival, and treatments thereof. METHODS: Cultured mouse brain bEnd5 endothelial cells were treated with integrin, FAK or STAT3 inhibitors, FAK siRNA, as well as integrin and STAT3 activators...
December 15, 2016: Cell Communication and Signaling: CCS
https://www.readbyqxmd.com/read/27965978/rebamipide-an-amino-acid-analog-of-2-1h-quinolinone-inhibits-the-formation-of-human-osteoclasts
#10
Yuki Nanke, Tsuyoshi Kobashigawa, Toru Yago, Manabu Kawamoto, Hisashi Yamanaka, Shigeru Kotake
Objectives. Drug repositioning or drug reprofiling (DR) has recently been growing in importance. DR has a significant advantage over traditional drug development because the repositioned drug has already passed toxicity tests; its safety is known, and the risk of adverse toxicology is reduced. In the current study, we investigated the role of rebamipide, a mucosa-protecting agent, with recently reported anti-inflammatory function, in human osteoclastogenesis. Methods. Peripheral blood mononuclear cells (PBMCs) were cultured in the presence of M-CSF and sRANKL...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27938493/thrombospondin-1-derived-peptide-rfyvvmwk-improves-the-adhesive-phenotype-of-cd34sup-sup-cells-from-atherosclerotic-patients-with-type-2-diabetes
#11
Sylvie Cointe, Éric Rhéaume, Catherine Martel, Olivier Blanc-Brude, Evemie Dubé, Florence Sabatier, Françoise Dignat-George, Jean-Claude Tardif, Arnaud Bonnefoy
<p>Background: CD34(+) progenitor cells are growingly used for vascular repair. However, in diabetic individuals with cardiovascular diseases, these cells have dysfunctional engraftment capabilities, which compromise their use for autologous cell therapy. The thrombospondin-1-derived peptide RFYVVMWK has previously been reported to stimulate cell adhesiveness through CD47 and integrin activation pathways.</p><p>Objectives: Our aim was to test whether RFYVVMWK preconditioning could modulate CD34(+) cells phenotype and enhance its pro-adhesive properties in diabetic patients...
6, 2016: Cell Transplantation
https://www.readbyqxmd.com/read/27913707/correction-antagonists-of-igf-vitronectin-interactions-inhibit-igf-i-induced-breast-cancer-cell-functions
#12
(no author information available yet)
No abstract text is available yet for this article.
December 2016: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/27896223/urokinase-type-plasminogen-activator-receptor-upar-as-a-new-therapeutic-target-in-cancer
#13
Nunzia Montuori, Ada Pesapane, Francesca W Rossi, Valentina Giudice, Amato De Paulis, Carmine Selleri, Pia Ragno
The urokinase (uPA)-type plasminogen activator receptor (uPAR) is a GPI-anchored receptor that focuses urokinase (uPA) proteolytic activity on the cell surface. uPAR also regulates cell adhesion, migration and proliferation, protects from apoptosis and contributes to epithelial mesenchymal transition (EMT), independently of uPA enzymatic activity. Indeed, uPAR interacts with beta1, beta2 and beta3 integrins, thus regulating their activities. uPAR cross-talks with receptor tyrosine kinases through integrins and regulates cancer cell dormancy, proliferation and angiogenesis...
November 2016: Translational Medicine @ UniSa
https://www.readbyqxmd.com/read/27871448/diagnostic-and-prognostic-value-of-serum-vitronectin-levels-in-human-glioma
#14
Mao-Hua Chen, Chuan Lu, Jun Sun, Xian-Dong Chen, Jun-Xia Dai, Jian-Yong Cai, Xiang-Lin Chen
OBJECTIVE: Vitronectin is an extracellular matrix protein, the synthesis of which by glioma cells correlates with tumor grade. The current study was designed to investigate the relationship between serum vitronectin levels and clinicopathological characteristics, diagnosis and prognosis in glioma patients. METHODS: In a prospective observatory study, a total of 98 glioma patients, 98 healthy controls, 98 other non-glioma brain tumors, and 98 other non-tumor neurological diseases were recruited...
December 15, 2016: Journal of the Neurological Sciences
https://www.readbyqxmd.com/read/27845768/selection-of-a-novel-aptamer-against-vitronectin-using-capillary-electrophoresis-and-next-generation-sequencing
#15
Christopher H Stuart, Kathryn R Riley, Olcay Boyacioglu, Denise M Herpai, Waldemar Debinski, Shadi Qasem, Frank C Marini, Christa L Colyer, William H Gmeiner
Breast cancer (BC) results in ~40,000 deaths each year in the United States and even among survivors treatment of the disease may have devastating consequences, including increased risk for heart disease and cognitive impairment resulting from the toxic effects of chemotherapy. Aptamer-mediated drug delivery can contribute to improved treatment outcomes through the selective delivery of chemotherapy to BC cells, provided suitable cancer-specific antigens can be identified. We report here the use of capillary electrophoresis in conjunction with next generation sequencing to develop the first vitronectin (VN) binding aptamer (VBA-01; Kd 405 nmol/l, the first aptamer to vitronectin (VN; Kd = 405 nmol/l) , a protein that plays an important role in wound healing and that is present at elevated levels in BC tissue and in the blood of BC patients relative to the corresponding nonmalignant tissues...
November 15, 2016: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/27833126/binary-colloidal-crystals-bccs-as-a-feeder-free-system-to-generate-human-induced-pluripotent-stem-cells-hipscs
#16
Peng-Yuan Wang, Sandy Shen-Chi Hung, Helmut Thissen, Peter Kingshott, Raymond Ching-Bong Wong
Human induced pluripotent stem cells (hiPSCs) are capable of differentiating into any cell type and provide significant advances to cell therapy and regenerative medicine. However, the current protocol for hiPSC generation is relatively inefficient and often results in many partially reprogrammed colonies, which increases the cost and reduces the applicability of hiPSCs. Biophysical stimulation, in particular from tuning cell-surface interactions, can trigger specific cellular responses that could in turn promote the reprogramming process...
November 11, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27830022/transplantation-of-bone-marrow-derived-mesenchymal-stem-cells-rescues-partially-rachitic-phenotypes-induced-by-1-25-dihydroxyvitamin-d-deficiency-in-mice
#17
Zengli Zhang, Shaomeng Yin, Xian Xue, Ji Ji, Jian Tong, David Goltzman, Dengshun Miao
To determine whether the transplantation of bone marrow-derived mesenchymal stem cells (BM-MSCs) can improve the 1,25(OH)2D deficiency-induced rachitic phenotype, 2×10(6) BM-MSCs from wild-type mice or vehicle were transplanted by tail vein injection into mice deficient in 1,25(OH)2D due to targeted deletion of 1α(OH)ase (1α(OH)ase(-/-)). Our results show that 1α(OH)ase mRNA was expressed in the BM-MSCs derived from wild-type mice, and was detected in long bone, kidney and intestine from BM-MSC-transplanted 1α(OH)ase(-/-) recipients...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27828845/early-effects-of-paroxysmal-atrial-fibrillation-on-plasma-markers-of-fibrinolysis
#18
Mariya Negreva, Svetoslav Georgiev, Katerina Vitlianova
There are no studies to date on the early changes in the hemostasis profile of patients with paroxysmal atrial fibrillation (PAF).Given the key role of the fibrinolytic system in maintaining blood fluidity, our aim was to examine its activity in patients with clinical manifestation of the disease <24 hours.We studied 51 nonanticoagulated patients with a first episode of the disease (26 men, 25 women; mean age 59.84 ± 1.60 years) and 52 controls (26 men, 26 women; mean age 59.50 ± 1.46 years) who matched the patients in terms of gender, age, comorbidities, and conducted treatment...
November 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27826074/anatomical-differences-of-the-protein-profile-in-the-rabbit-sclera-during-growth
#19
Ying Liu, Pingbo Zhang, Jianbo Pan, Mark Augustine D'Souza, Craig P Dufresne, Richard D Semba, Jiang Qian, Deepak P Edward
We aimed to investigate the proteome changes in anatomical regions of sclera during growth and development of the rabbit. Sclera from New Zealand white rabbits of three ages (1 month, 2 months and 6 months) was dissected into three segments - anterior, equatorial, and posterior. A total of 36 samples were divided into groups by age and anatomical region. Tryptic digests of total proteins were analyzed by liquid chromatography followed by tandem mass spectrometry (LC-MS/MS). Label-free quantification based on spectral counts was used to determine the relative protein abundance and identify proteins with statistically significant differences between age groups or anatomical regions of the sclera...
November 5, 2016: Experimental Eye Research
https://www.readbyqxmd.com/read/27808427/integrin-%C3%AE-v-%C3%AE-8-adopts-a-high-affinity-state-for-soluble-ligands-under-physiological-conditions
#20
Ping Hu, Bing-Hao Luo
It has been proposed that integrins adopt a low affinity conformation under physiological conditions. Integrin can either be activated through cytoplasm or by binding of cations such as Mn(2+) to the head domain. The cytoplasmic activation pathway, i.e. inside-out signaling has been regarded as the physiological pathway for integrin activation. Integrin β8 is important for neuron vascular development. However, due to the highly divergent cytoplasmic domain, this integrin probably does not rely on inside-out signaling for affinity regulation...
November 3, 2016: Journal of Cellular Biochemistry
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