keyword
MENU ▼
Read by QxMD icon Read
search

Vitronectin

keyword
https://www.readbyqxmd.com/read/29474926/the-fibrogenic-actions-of-the-coagulant-and-plasminogen-activation-systems-in-pulmonary-fibrosis
#1
REVIEW
Michael Schuliga, Christopher Grainge, Glen Westall, Darryl Knight
Fibrosis causes irreversible damage to lung structure and function in restrictive lung diseases such as idiopathic pulmonary fibrosis (IPF). Extravascular coagulation involving fibrin formation in the intra-alveolar compartment is postulated to have a pivotal role in the development of pulmonary fibrosis, serving as a provisional matrix for migrating fibroblasts. Furthermore, proteases of the coagulation and plasminogen activation (plasminergic) systems that form and breakdown fibrin respectively directly contribute to pulmonary fibrosis...
February 20, 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29463024/overexpression-of-the-vitronectin-v10-subunit-in-patients-with-nonalcoholic-steatohepatitis-implications-for-noninvasive-diagnosis-of-nash
#2
Maria Del Ben, Diletta Overi, Licia Polimeni, Guido Carpino, Giancarlo Labbadia, Francesco Baratta, Daniele Pastori, Valeria Noce, Eugenio Gaudio, Francesco Angelico, Carmine Mancone
Nonalcoholic steatohepatitis (NASH) is the critical stage of nonalcoholic fatty liver disease (NAFLD). The persistence of necroinflammatory lesions and fibrogenesis in NASH is the leading cause of liver cirrhosis and, ultimately, hepatocellular carcinoma. To date, the histological examination of liver biopsies, albeit invasive, remains the means to distinguish NASH from simple steatosis (NAFL). Therefore, a noninvasive diagnosis by serum biomarkers is eagerly needed. Here, by a proteomic approach, we analysed the soluble low-molecular-weight protein fragments flushed out from the liver tissue of NAFL and NASH patients...
February 18, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29427759/quantitative-analysis-of-core-fucosylation-of-serum-proteins-in-liver-diseases-by-lc-ms-mrm
#3
Junfeng Ma, Miloslav Sanda, Renhuizi Wei, Lihua Zhang, Radoslav Goldman
Aberrant core fucosylation of proteins has been linked to liver diseases. In this study, we carried out multiple reaction monitoring (MRM) quantification of core fucosylated N-glycopeptides of serum proteins partially deglycosylated by a combination of endoglycosidases (endoF1, endoF2, and endoF3). To minimize variability associated with the preparatory steps, the analysis was performed without enrichment of glycopeptides or fractionation of serum besides the nanoRP chromatography. Specifically, we quantified core fucosylation of 22 N-glycopeptides derived from 17 proteins together with protein abundance of these glycoproteins in a cohort of 45 participants (15 disease-free control, 15 fibrosis and 15 cirrhosis patients) using a multiplex nanoUPLC-MS-MRM workflow...
February 7, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29410615/leptin-receptor-expression-in-mouse-intracranial-perivascular-cells
#4
Xuefeng Yuan, Alexandre Caron, Hua Wu, Laurent Gautron
Past studies have suggested that non-neuronal brain cells express the leptin receptor. However, the identity and distribution of these leptin receptor-expressing non-neuronal brain cells remain debated. This study assessed the distribution of the long form of the leptin receptor (LepRb) in non-neuronal brain cells using a reporter mouse model in which LepRb-expressing cells are permanently marked by tdTomato fluorescent protein (LepRb-CretdTomato ). Double immunohistochemistry revealed that, in agreement with the literature, the vast majority of tdTomato-tagged cells across the mouse brain were neurons (i...
2018: Frontiers in Neuroanatomy
https://www.readbyqxmd.com/read/29399943/high-throughput-serum-proteomics-for-the-identification-of-protein-biomarkers-of-mortality-in-older-men
#5
Eric S Orwoll, Jack Wiedrick, Jon Jacobs, Erin S Baker, Paul Piehowski, Vladislav Petyuk, Yuqian Gao, Tujin Shi, Richard D Smith, Douglas C Bauer, Steven R Cummings, Carrie M Nielson, Jodi Lapidus
The biological perturbations associated with incident mortality are not well elucidated, and there are limited biomarkers for the prediction of mortality. We used a novel high-throughput proteomics approach to identify serum peptides and proteins associated with 5-year mortality in community-dwelling men age ≥65 years who participated in a longitudinal observational study of musculoskeletal aging (Osteoporotic Fractures in Men: MrOS). In a discovery phase, serum specimens collected at baseline in 2473 men were analyzed using liquid chromatography-ion mobility-mass spectrometry, and incident mortality in the subsequent 5 years was ascertained by tri-annual questionnaire...
February 5, 2018: Aging Cell
https://www.readbyqxmd.com/read/29398086/autolysin-mediated-adherence-of-staphylococcus-aureus-with-fibronectin-gelatin-and-heparin
#6
Chandni Porayath, Maneesha K Suresh, Raja Biswas, Bipin G Nair, Nandita Mishra, Sanjay Pal
Major autolysin (Atl) of Staphylococcus aureusis a cell surface associated peptidoglycan hydrolase with amidase and glucosaminidase domains. Atl enzymes (amidase and glucosaminidase) are known to participate in biofilm formation and also can bind with host matrices. Earlier studies demonstrated the binding of Atlwithfibronectin, thrombospondin 1, vitronectin and heat shock cognate protein Hsc70. Here, we have shown, Atl mediates attachment of S.aureus to heparin and gelatine as well. The atl mutant strain demonstrated around 2...
February 2, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29382358/integrin-upar-signaling-leads-to-fra-1-phosphorylation-and-enhanced-breast-cancer-invasion
#7
Matthew G Annis, Veronique Ouellet, Jonathan P Rennhack, Sylvain L'Esperance, Claudine Rancourt, Anne-Marie Mes-Masson, Eran R Andrechek, Peter M Siegel
BACKGROUND: The Fos-related antigen 1 (FRA-1) transcription factor promotes tumor cell growth, invasion and metastasis. Phosphorylation of FRA-1 increases protein stability and function. We identify a novel signaling axis that leads to increased phosphorylation of FRA-1, increased extracellular matrix (ECM)-induced breast cancer cell invasion and is prognostic of poor outcome in patients with breast cancer. METHODS: While characterizing five breast cancer cell lines derived from primary human breast tumors, we identified BRC-31 as a novel basal-like cell model that expresses elevated FRA-1 levels...
January 30, 2018: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/29369695/integrin-heterodimers-expressed-on-the-surface-of-porcine-spermatogonial-stem-cells
#8
Min Hee Park, Min Seong Kim, Jung Im Yun, Jung Hoon Choi, Eunsong Lee, Seung Tae Lee
To date, in vitro culture systems able to sufficiently expand the small population of spermatogonial stem cells (SSCs), a tool for the development of sperm-mediated gene transfer techniques in transgenic pigs, in the porcine seminiferous tubule have not been reported. Therefore, as a step toward engineering a noncellular niche to support the in vitro maintenance of porcine SSC self-renewal, we investigated the types of integrin heterodimers that are expressed and functional on their membrane. The α and β integrin subunit protein expressions were analyzed using immunocytochemistry and fluorescence immunoassay, and the function of integrin heterodimers was confirmed by attachment and antibody inhibition assays...
January 25, 2018: DNA and Cell Biology
https://www.readbyqxmd.com/read/29366178/modulation-of-human-vsmc-migration-by-vitronectin-studies-of-transcriptional-regulation
#9
Íngrid Martí-Pàmies, Laia Cañes, Cristina Rodriguez, José Martínez-González
No abstract text is available yet for this article.
August 2017: Atherosclerosis
https://www.readbyqxmd.com/read/29342970/the-possible-importance-of-%C3%AE-3-integrins-for-leukemogenesis-and-chemoresistance-in-acute-myeloid-leukemia
#10
REVIEW
Silje Johansen, Annette K Brenner, Sushma Bartaula-Brevik, Håkon Reikvam, Øystein Bruserud
Acute myeloid leukemia (AML) is an aggressive bone marrow malignancy where the immature leukemia cells communicate with neighboring cells through constitutive cytokine release and through their cell surface adhesion molecules. The primary AML cells express various integrins. These heterodimeric molecules containing an α and a β chain are cell surface molecules that bind extracellular matrix molecules, cell surface molecules and soluble mediators. The β3 integrin (ITGB3) chain can form heterodimers only with the two α chains αIIb and αV...
January 15, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29340063/a-molecular-signature-of-dormancy-in-cd34-cd38-acute-myeloid-leukaemia-cells
#11
Mazin Gh Al-Asadi, Grace Brindle, Marcos Castellanos, Sean T May, Ken I Mills, Nigel H Russell, Claire H Seedhouse, Monica Pallis
Dormant leukaemia initiating cells in the bone marrow niche are a crucial therapeutic target for total eradication of acute myeloid leukaemia. To study this cellular subset we created and validated an in vitro model employing the cell line TF-1a, treated with Transforming Growth Factor β1 (TGFβ1) and a mammalian target of rapamycin inhibitor. The treated cells showed decreases in total RNA, Ki-67 and CD71, increased aldehyde dehydrogenase activity, forkhead box 03A (FOX03A) nuclear translocation and growth inhibition, with no evidence of apoptosis or differentiation...
December 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29338828/screening-serum-differential-proteins-for-childhood-asthma-at-different-control-levels-by-isobaric-tags-for-relative-and-absolute-quantification-based-proteomic-technology
#12
Ji Yan Liu, Ying Wang, Yuan Zhao Wang, Jia Wen Zhu, Fang Dian Dai
Objective To screen serum differential proteins for childhood asthma at different control levels,which provided the basis for the prevention and treatment of childhood asthma. Methods Isobaric tags for relative and absolute quantification,two-dimensional liquid chromatography,nanoelectrospray ionization,and high-resolution tandem mass spectrometry using the hybrid quadrupole time-of-flight platform was used to screen the differential proteins in serum samples from pediatric patients with controlled,partly controlled,or uncontrolled childhood asthma...
December 20, 2017: Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
https://www.readbyqxmd.com/read/29330502/targeting-insulin-like-growth-factor-i-and-extracellular-matrix-interactions-in-melanoma-progression
#13
Berline Murekatete, Ali Shokoohmand, Jacqui McGovern, Lipsa Mohanty, Christoph Meinert, Brett G Hollier, Alfred Zippelius, Zee Upton, Abhishek S Kashyap
Insulin-like growth factor (IGF)-I binds to the ECM protein vitronectin (VN) through IGF binding proteins (IGFBPs) to enhance proliferation and migration of skin keratinocytes and fibroblasts. Although evidence exists for the role of individual components of the complex (IGF-I, IGFBP-3 and VN), the cellular functions stimulated by these proteins together as a complex remains un-investigated in melanoma cells. We report here that the IGF-I:IGFBP-3:VN trimeric complex stimulates a dose-dependent increase in the proliferation and migration of WM35 and Sk-MEL28 melanoma cells...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29320683/changes-in-cholesterol-level-alter-integrin-sequestration-in-raft-mimicking-lipid-mixtures
#14
Yifan Ge, Jiayun Gao, Rainer Jordan, Christoph A Naumann
The influence of cholesterol (CHOL) level on integrin sequestration in raft-mimicking lipid mixtures forming coexisting liquid-ordered (lo) and liquid-disordered (ld) lipid domains is investigated using complementary, single-molecule-sensitive, confocal detection methods. Systematic analysis of membrane protein distribution in such a model membrane environment demonstrates that variation of CHOL level has a profound influence on lo-ld sequestration of integrins, thereby exhibiting overall ld preference in the absence of ligands and lo affinity upon vitronectin addition...
January 9, 2018: Biophysical Journal
https://www.readbyqxmd.com/read/29286443/reprogramming-primary-amniotic-fluid-and-membrane-cells-to-pluripotency-in-xeno-free-conditions
#15
Jaroslav Slamecka, Javier Laurini, Troy Shirley, Simon Philipp Hoerstrup, Benedikt Weber, Laurie Owen, Steven McClellan
Autologous cell-based therapies got a step closer to reality with the introduction of induced pluripotent stem cells. Fetal stem cells, such as amniotic fluid and membrane mesenchymal stem cells, represent a unique type of undifferentiated cells with promise in tissue engineering and for reprogramming into iPSC for future pediatric interventions and stem cell banking. The protocol presented here describes an optimized procedure for extracting and culturing primary amniotic fluid and membrane mesenchymal stem cells and generating episomal induced pluripotent stem cells from these cells in fully chemically defined culture conditions utilizing human recombinant vitronectin and the E8 medium...
November 27, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29276918/-analysis-of-urine-proteome-in-patients-with-postinfarction-cardiosclerosis-combined-with-hypertensive-disease-for-assessing-endothelial-dysfunction
#16
V P Masenko, L K Pastushkova, D N Kashirina, I V Dobrokhotov, A S Kononikhin, E S Tiys, V A Ivanisenko, O M Veselova, O N Vyborov, A M Nosovskiy, I N Goncharov, E N Nikolaev, I M Larina
In our study urine protein composition of 18 healthy volunteers was compared with that of 18 patients with ischemic heart disease and concomitant hypertension. Liquid chromatography-mass-spectrometry (LC-MS) analysis of the second fraction of morning urine was carried out using nano-line high performance liquid chromatograph and hybrid mass spectrometer. The analysis revealed 23 proteins expressed in the endothelium, according to the information contained in the database Bgee, and 49 proteins, with direct functional link with the processes in the endothelium in the reconstruction of associative networks using ANDSystem program...
November 2017: Kardiologiia
https://www.readbyqxmd.com/read/29250784/survey-for-the-presence-of-a-vitronectin-like-protein-in-micro-and-macroalgae-and-cyanobacteria
#17
Lacey M Field, Wayne R Fagerberg, S Anne Böttger
Vitronectin is a glycoprotein which serves a wide variety of roles in multicellular organisms. It was first identified in multicellular animals but has also been isolated from land plants and some algae where it appears to serve as an extracellular adhesive molecule. In order to further elucidate presence and localization of a vitronectin-like protein and its potential role in algae we surveyed different morphological regions of 24 species of macro- and microalgae and three species of cyanobacteria for the presence of a vitronectin-like protein...
December 18, 2017: Journal of Phycology
https://www.readbyqxmd.com/read/29250746/neutrophils-in-the-lung-the-first-responders
#18
REVIEW
Gurpreet Kaur Aulakh
Neutrophils, constituting the first line of defense, perform vital functions during immune surveillance. A key feature that assists in their prompt response to an inflammatory signal is rapid migration to the affected site. They are normally short-lived but can be activated to live longer under the influence of an inflammatory stimulus. They can, thereby, release their toxic granule contents that are differentially housed inside the cytoplasm. Although these events are well characterized in the peripheral circulation, we are still far from fully understanding their recruitment in lungs...
December 18, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/29222114/blood-vitronectin-is-a-major-activator-of-lif-and-il-6-in-the-brain-through-integrin-fak-and-upar-signaling
#19
M P Keasey, C Jia, L F Pimentel, R S Sante, C Lovins, T Hagg
We defined how blood-derived vitronectin (VTN) rapidly and potently activates leukemia inhibitory factor (LIF) and pro-inflammatory interleukin 6 (IL-6) in vitro and after vascular injury in the brain. VTN (not fibrinogen, fibronectin, laminin-111, collagen-I) robustly increased LIF and IL-6 within 4 hr in C6-astroglioma cells, while VTN-/- mouse plasma was less effective than wildtype. LIF and IL-6 were induced by intracerebral injection of rhVTN in mice, but less by intracerebral hemorrhage in VTN-/- than wildtype littermates...
December 8, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/29215086/genetic-predisposition-to-infection-in-a-case-of-atypical-hemolytic-uremic-syndrome
#20
Lambertus van den Heuvel, Kristian Riesbeck, Omaima El Tahir, Valentina Gracchi, Mariann Kremlitzka, Servaas A Morré, A Marceline van Furth, Birendra Singh, Marcin Okrój, Nicole van de Kar, Anna M Blom, Elena Volokhina
Most cases of hemolytic uremic syndrome (HUS) are caused by infection with enterohemorrhagic Escherichia coli (EHEC). Genetic defects causing uncontrolled complement activation are associated with the more severe atypical HUS (aHUS). Non-EHEC infections can trigger the disease, however, complement defects predisposing to such infections have not yet been studied. We describe a 2-month-old patient infected with different Gram-negative bacterial species resulting in aHUS. Serum analysis revealed slow complement activation kinetics...
November 13, 2017: Journal of Human Genetics
keyword
keyword
7620
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"