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https://www.readbyqxmd.com/read/28728360/isolation-and-characterization-of-a-novel-gossypol-degrading-bacteria-bacillus-subtilis-strain-rbs
#1
Yunhua Zhang, Lijuan Chen, Zhengyou Zhang, Li Dai, Ying Liu, Maoji Cheng
Objective: An experiment was conducted to isolate gossypol-degrading bacteria and assessed its potential for gossypol degradation. Methods: Rumen liquid was collected from the rumen of cows through fistula from the experimental pasture. Approximately 1 ml of the rumen liquid was spread onto basal medium plates containing 2 g/l gossypol as the only source of carbon and was then cultured at 39°C to isolate gossypol-degrading bacteria. The isolated colonies were cultured for 6 h and then observed their size and shape by the microscope and scanning electron microscope...
June 27, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28727811/factors-limiting-sulfolane-biodegradation-in-contaminated-subarctic-aquifer-substrate
#2
Christopher P Kasanke, Mary Beth Leigh
Sulfolane, a water-soluble organosulfur compound, is used industrially worldwide and is associated with one of the largest contaminated groundwater plumes in the state of Alaska. Despite being widely used, little is understood about the degradation of sulfolane in the environment, especially in cold regions. We conducted aerobic and anaerobic microcosm studies to assess the biological and abiotic sulfolane degradation potential of contaminated subarctic aquifer groundwater and sediment from Interior Alaska...
2017: PloS One
https://www.readbyqxmd.com/read/28727446/reductive-outer-sphere-single-electron-transfer-is-an-exception-rather-than-the-rule-in-natural-and-engineered-chlorinated-ethene-dehalogenation
#3
Benjamin Heckel, Stefan Cretnik, Sarah Kliegman, Orfan Shouakar-Stash, Kristopher McNeill, Martin Elsner
Chlorinated ethenes (CEs) such as perchloroethylene, trichloroethylene and dichloroethylene are notorious groundwater contaminants. Although reductive dehalogenation is key to their environmental and engineered degradation, underlying reaction mechanisms remain elusive. Outer-sphere reductive single electron transfer (OS-SET) has been proposed for such different processes as Vitamin B12-dependent biodegradation and zero-valent metal-mediated dehalogenation. Compound-specific isotope effect (13C/12C, 37Cl/35Cl) analysis offers a new opportunity to test these hypotheses...
July 20, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28726229/monitoring-and-modeling-4-chlorophenol-biodegradation-kinetics-by-phenol-acclimated-activated-sludge-by-using-open-respirometry
#4
Cintia C Lobo, Nora C Bertola, Edgardo M Contreras, Noemí E Zaritzky
The aim of this study was to analyze the mechanisms, stoichiometry, and stability of 4-chlorophenol (4CP) biodegradation kinetics by phenol-acclimated activated sludge using open respirometry. While the removal of 4CP was higher than 98%, the removal of chemical oxygen demand (COD) ranged between 69 and 79% due to the accumulation of an intermediate metabolite. The value obtained from respirometric profiles for the stoichiometric ratio of O2 to 4CP (YO2/4CP) was 1.95 ± 0.04 mol of oxygen consumed per mol of 4CP removed...
July 20, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28726220/effect-of-operational-factors-on-bioregeneration-of-binary-phenol-and-4-chlorophenol-loaded-granular-activated-carbon-using-pva-immobilized-biomass-cryogels
#5
Kwok-Yii Leong, Rohana Adnan, Poh-Eng Lim, Si-Ling Ng, Chye-Eng Seng
The effects of dry biomass density in cryogel beads, shaking speed and initial concentration ratio of phenol to 4-chlorophenol (4-CP) on the bioregeneration efficiencies of binary phenol and 4-CP-loaded granular activated carbon (GAC) for phenol and 4-CP, respectively, were investigated under the simultaneous adsorption and biodegradation approach. The results revealed higher bioregeneration efficiencies of binary-loaded GAC for phenol and 4-CP at higher dry biomass density but moderate shaking speed. The optimum dry biomass density in cryogel beads and shaking speed for use in bioregeneration were found to be 0...
July 18, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28725934/-scaffold-based-bone-tissue-engineering
#6
REVIEW
B M Holzapfel, M Rudert, D W Hutmacher
Tissue engineering provides the possibility of regenerating damaged or lost osseous structures without the need for permanent implants. Within this context, biodegradable and bioresorbable scaffolds can provide structural and biomechanical stability until the body's own tissue can take over their function. Additive biomanufacturing makes it possible to design the scaffold's architectural characteristics to specifically guide tissue formation and regeneration. Its nano-, micro-, and macro-architectural properties can be tailored to ensure vascularization, oxygenation, nutrient supply, waste exchange, and eventually ossification not only in its periphery but also in its center, which is not in direct contact with osteogenic elements of the surrounding healthy tissue...
July 19, 2017: Der Orthopäde
https://www.readbyqxmd.com/read/28725529/recent-progress-on-piezoelectric-and-triboelectric-energy-harvesters-in-biomedical-systems
#7
REVIEW
Qiang Zheng, Bojing Shi, Zhou Li, Zhong Lin Wang
Implantable medical devices (IMDs) have become indispensable medical tools for improving the quality of life and prolonging the patient's lifespan. The minimization and extension of lifetime are main challenges for the development of IMDs. Current innovative research on this topic is focused on internal charging using the energy generated by the physiological environment or natural body activity. To harvest biomechanical energy efficiently, piezoelectric and triboelectric energy harvesters with sophisticated structural and material design have been developed...
July 2017: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://www.readbyqxmd.com/read/28725150/development-and-evaluation-of-ketoprofen-sustained-release-matrix-tablet-using-hibiscus-rosa-sinensis-leaves-mucilage
#8
M Kaleemullah, K Jiyauddin, E Thiban, S Rasha, S Al-Dhalli, S Budiasih, O E Gamal, A Fadli, Y Eddy
Currently, the use of natural gums and mucilage is of increasing importance in pharmaceutical formulations as valuable drug excipient. Natural plant-based materials are economic, free of side effects, biocompatible and biodegradable. Therefore, Ketoprofen matrix tablets were formulated by employing Hibiscus rosa-sinensis leaves mucilage as natural polymer and HPMC (K100M) as a synthetic polymer to sustain the drug release from matrix system. Direct compression method was used to develop sustained released matrix tablets...
July 2017: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://www.readbyqxmd.com/read/28724292/adsorption-of-proteins-on-colloidal-lignin-particles-for-advanced-biomaterials
#9
Timo Leskinen, Joanna Witos, Juan José Valle Delgado, Kalle Sakari Lintinen, Mauri A Kostiainen, Susanne Kristina Wiedmer, Monika Österberg, Maija-Liisa Mattinen
Coating of colloidal lignin particles (CLPs), or lignin nanoparticles (LNPs), with proteins was evaluated in order to establish a safe, self-assembly-mediated modification technique to tune their surface chemistry. Gelatin and poly- L-lysine formed the most pronounced protein corona on the CLP surface, as determined by dynamic light scattering (DLS) and zeta potential measurements. Spherical morphology of individual protein coated CLPs was confirmed by transmission electron (TEM) and atomic force (AFM) microscopy...
July 19, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28724255/effects-of-organic-composition-on-the-anaerobic-biodegradability-of-food-waste
#10
Yangyang Li, Yiying Jin, Aiduan Borrion, Hailong Li, Jinhui Li
This work investigated the influence of carbohydrates, proteins and lipids on the anaerobic digestion of food waste (FW) and the relationship between the parameters characterising digestion. Increasing the concentrations of proteins and lipids, and decreasing carbohydrate content in FW, led to high buffering capacity, reduction of proteins (52.7-65.0%) and lipids (57.4-88.2%), and methane production (385-627 mLCH4/g volatile solid), while achieving a short retention time. There were no significant correlations between the reduction of organics, hydrolysis rate constant (0...
July 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28724094/temperature-impacts-on-soil-microbial-communities-and-potential-implications-for-the-biodegradation-of-turfgrass-pesticides
#11
Lisa M Reedich, Michael D Millican, Paul L Koch
Maintaining healthy turfgrass often results in the use of pesticides to manage weed, insect, and disease pests. To identify and understand potential nontarget impacts of pesticide usage while still maintaining attractive and functional turfgrass sites, it is important to improve our understanding of how pesticides degrade in various environments throughout the growing season. Temperature heavily influences microbial community composition and activity, and the microbial community often heavily influences pesticide degradation in soil ecosystems...
May 2017: Journal of Environmental Quality
https://www.readbyqxmd.com/read/28723068/biomimetic-hybridization-of-kevlar-into-silk-fibroin-nanofibrous-strategy-for-improved-mechanic-properties-of-flexible-composites-and-filtration-membranes
#12
Lili Lv, Xiangsheng Han, Lu Zong, Mingjie Li, Jun You, Xiaochen Wu, Chaoxu Li
Silk, one of the strongest natural biopolymers, was hybridized with Kevlar, one of the strongest synthetic polymers, through a biomimetic nanofibrous strategy. Regenerated silk materials have outstanding properties in transparency, biocompatibility, biodegradability and sustainability, and promised the applications as diverse as in pharmaceutics, electronics, photonic devices and membranes. To compete with super mechanic properties of their natural counterpart, regenerated silk materials have been hybridized with inorganic fillers such as graphene and carbon nanotubes, but frequently losing essential mechanic flexibility...
July 19, 2017: ACS Nano
https://www.readbyqxmd.com/read/28722060/poly-amino-acid-polyester-graft-copolymer-nanoparticles-for-the-acid-mediated-release-of-doxorubicin
#13
Daniel J Price, Mthulisi Khuphe, Robert P W Davies, James R McLaughlan, Nicola Ingram, Paul D Thornton
Biodegradable polymers have emerged as highly effective drug delivery vehicles. We combine N-carboxyanhydride and O-carboxyanhydride ring opening polymerisations to synthesise a poly(amino acid)-polyester graft copolymer capable of encapsulating, and subsequently releasing doxorubicin via acid-mediated hydrolysis. Consequently, the nanoparticles detailed are extremely promising vehicles for the controlled delivery of chemotherapeutic agents.
July 19, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28721731/self-assembled-injectable-peptide-hydrogels-capable-of-triggering-antitumor-immune-response
#14
Ruirui Xing, Shukun Li, Ning Zhang, Guizhi Shen, Helmuth Möhwald, Xuehai Yan
Self-assembled peptide hydrogels are particularly appealing for drug delivery, tissue engineering, and anti-tumor therapy due to various advantageous features including excellent biocompatibility and biodegradability, defined molecular and higher organized structures and easy availability. However, the poor mechanical and rheological properties of assembled peptide hydrogels cause difficulties in injection, thus limiting further applications. Herein, injectable peptide-based hydrogels with tunable mechanical and rheological properties were obtained by combination with a positively charged poly-peptide (poly-L-lysine, PLL)...
July 19, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28721657/biological-decolorization-and-degradation-of-malachite-green-by-pseudomonas-sp-yb2-process-optimization-and-biodegradation-pathway
#15
Yanbin Tao, Fei Wang, Lingjun Meng, Yang Guo, Mengshu Han, Jinhai Li, Cheng Sun, Shaomang Wang
A novel strain Pseudomonas sp. YB2, which was isolated from activated sludge, was studied for biological decolorization and degradation of malachite green (MG). The results show that this aerobic strain is an aerobic bacterium, which has relatively high salt tolerance and high antibiotic resistance. The optimal performance condition for both bacterial growth and decolorization of MG was at 25-35 °C and pH 5.0-7.0. It could efficiently decolorize 90.40% MG at a high concentration up to 1500 mg/L in 24 h, and concentrations of MG lower than 1000 mg/L could be completely decolorized in 12 h...
July 18, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28721617/effect-of-membrane-property-and-feed-water-organic-matter-quality-on-long-term-performance-of-the-gravity-driven-membrane-filtration-process
#16
Dongwhi Lee, Yuri Lee, Shin Sik Choi, Sang-Ho Lee, Kyoung-Woong Kim, Yunho Lee
This study investigated the influence of membrane property and feed water organic matter quality on the permeate flux and water quality during gravity-driven membrane (GDM) filtration. GDM filtration was continuously carried out over 500 days at hydrostatic pressure of 65 mbar in dead-end mode without any back-flushing or membrane cleaning. Three ultrafiltration (UF) membranes (PES-100 kDa, PVDF-120 kDa, and PVDF-100 kDa) and one microfiltration (MF) membrane (PTFE-0.3 μm) were tested for treating lake water with varied organic matter qualities due to algal growth...
July 18, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28721045/synthesis-construction-and-evaluation-of-self-assembled-nano-bacitracin-a-as-an-efficient-antibacterial-agent-in-vitro-and-in-vivo
#17
Wei Hong, Xiang Gao, Peng Qiu, Jie Yang, Mingxi Qiao, Hong Shi, Dexian Zhang, Chunlian Tian, Shengli Niu, Mingchun Liu
Bacitracin A (BA) is an excellent polypeptide antibiotic that is active against gram-positive bacteria without triggering multidrug resistance. However, BA is inactive against gram-negative bacteria because of its inability to cross the outer membrane of these cells, and it has strong nephrotoxicity, thus limiting its clinical applications. Nanoantibiotics can effectively localize antibiotics to the periplasmic space of bacteria while decreasing the adverse effects of antibiotics. In this study, biodegradable hydrophobic copolymers of poly (d,l-lactide-co-glycolide) (PLGA) were attached to the N-termini of BA to design a novel class of self-assembled nano-bacitracin A (nano-BAs), and their potential as antibacterial agents was evaluated in vitro and in vivo...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28720895/rapid-response-of-eastern-mediterranean-deep-sea-microbial-communities-to-oil
#18
Jiang Liu, Stephen M Techtmann, Hannah L Woo, Daliang Ning, Julian L Fortney, Terry C Hazen
Deep marine oil spills like the Deepwater Horizon (DWH) in the Gulf of Mexico have the potential to drastically impact marine systems. Crude oil contamination in marine systems remains a concern, especially for countries around the Mediterranean Sea with off shore oil production. The goal of this study was to investigate the response of indigenous microbial communities to crude oil in the deep Eastern Mediterranean Sea (E. Med.) water column and to minimize potential bias associated with storage and shifts in microbial community structure from sample storage...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28720867/graphene-microfiber-as-a-scaffold-for-regulation-of-neural-stem-cells-differentiation
#19
Weibo Guo, Jichuan Qiu, Jingquan Liu, Hong Liu
We report the cytocompatibility and regulating effects of the nanostructured reduced graphene oxide (rGO) microfibers, which are synthesized through a capillary hydrothermal method, on neural differentiation of neural stem cells (NSCs). Our findings indicate that the flexible, mechanically strong, surface nanoporous, biodegradable, and cytocompatible nanostructured rGO microfibers not only offer a more powerful substrate for NSCs adhesion and proliferation compared with 2D graphene film and tissue cluture plate but also regulate the NSCs differentiation into neurons and form a dense neural network surrounding the microfiber...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28720419/isolation-of-two-ochrobactrum-sp-strains-capable-of-degrading-the-nootropic-drug-piracetam
#20
Marta Woźniak-Karczewska, Monika Čvančarová, Łukasz Chrzanowski, Boris Kolvenbach, Philippe F-X Corvini, Danuta Cichocka
Piracetam (2-oxo-1-pyrrolidine acetamide) is a popular cognitive enhancer, which has recently been detected in waste and drinking water. Nootropic drugs are designed to affect human metabolism and act on the nervous system, but their environmental effects have yet to be the subject of detailed studies. In this report, we present the efficient biodegradation of the cognitive enhancer, piracetam. Two bacterial strains capable of using this compound as the sole carbon source were isolated and later identified as Ochrobactrum anthropi strain MW6 and Ochrobactrum intermedium strain MW7...
July 15, 2017: New Biotechnology
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