keyword
MENU ▼
Read by QxMD icon Read
search

Lipase lid

keyword
https://www.readbyqxmd.com/read/28106397/kinetics-of-detergent-induced-activation-and-inhibition-of-a-minimal-lipase
#1
Daniel Kübler, Anna Bergmann, Lukas Weger, Kim N Ingenbosch, Kerstin Hoffmann-Jacobsen
Detergents are commonly applied in lipase assays to solubilize sparingly soluble model substrates. However, detergents affect lipases as well as substrates in multiple ways. The effect of detergents on lipase activity is commonly attributed to conformational changes in the lid region. This study deals with the effect of the nonionic detergent, poly(ethylene glycol) dodecyl ether, on a lipase that does not contain a lid sequence, lipase A from Bacillus subtilis (BSLA). We show that BSLA activity depends strongly on the detergent concentration and the dependency profile changes with pH...
February 6, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28088576/lid-domain-plasticity-and-lipid-flexibility-modulate-enzyme-specificity-in-human-monoacylglycerol-lipase
#2
Laura Riccardi, Jose M Arencibia, Luca Bono, Andrea Armirotti, Stefania Girotto, Marco De Vivo
Human monoacylglycerol lipase (MAGL) is a membrane-interacting enzyme that generates pro-inflammatory signaling molecules. For this reason, MAGL inhibition is a promising strategy to treat pain, cancer, and neuroinflammatory diseases. MAGL can hydrolyze monoacylglycerols bearing an acyl chain of different lengths and degrees of unsaturation, cleaving primarily the endocannabinoid 2-arachidonoylglycerol. Importantly, the enzymatic binding site of MAGL is confined by a 75-amino-acid-long, flexible cap domain, named 'lid domain', which is structurally similar to that found in several other lipases...
January 12, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28063495/probing-conformational-changes-and-interfacial-recognition-site-of-lipases-with-surfactants-and-inhibitors
#3
E Mateos-Diaz, S Amara, A Roussel, S Longhi, C Cambillau, F Carrière
Structural studies on lipases by X-ray crystallography have revealed conformational changes occurring in the presence of surfactants/inhibitors and the pivotal role played by a molecular "lid" of variable size and structure depending on the enzyme. Besides controlling the access to the enzyme active site, the lid is involved in lipase activation, formation of the interfacial recognition site (IRS), and substrate docking within the active site. The combined use of surfactants and inhibitors has been critical for a better understanding of lipase structure-function relationships...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28049436/evolutionary-history-of-versatile-lipases-from-agaricales-through-reconstruction-of-ancestral-structures
#4
Jorge Barriuso, María Jesús Martínez
BACKGROUND: Fungal "Versatile carboxylic ester hydrolases" are enzymes with great biotechnological interest. Here we carried out a bioinformatic screening to find these proteins in genomes from Agaricales, by means of searching for conserved motifs, sequence and phylogenetic analysis, and three-dimensional modeling. Moreover, we reconstructed the molecular evolution of these enzymes along the time by inferring and analyzing the sequence of ancestral intermediate forms. RESULTS: The properties of the ancestral candidates are discussed on the basis of their three-dimensional structural models, the hydrophobicity of the lid, and the substrate binding intramolecular tunnel, revealing all of them featured properties of these enzymes...
January 3, 2017: BMC Genomics
https://www.readbyqxmd.com/read/27993564/interfacial-activation-of-m37-lipase-a-multi-scale-simulation-study
#5
Nathalie Willems, Mickaël Lelimousin, Heidi Koldsø, Mark S P Sansom
Lipases are enzymes of biotechnological importance that function at the interface formed between hydrophobic and aqueous environments. Hydrophobic interfaces can induce structural transitions in lipases that result in an increase in enzyme activity, although the detailed mechanism of this process is currently not well understood for many lipases. Here, we present a multi-scale molecular dynamics simulation study of how different interfaces affect the conformational dynamics of the psychrophilic lipase M37. Our simulations show that M37 lipase is able to interact both with anionic lipid bilayers and with triglyceride surfaces...
March 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27908755/synthesis-evaluation-and-molecular-modelling-studies-of-2-carbazol-3-yl-2-oxoacetamide-analogues-as-a-new-class-of-potential-pancreatic-lipase-inhibitors
#6
S N C Sridhar, George Ginson, P O Venkataramana Reddy, Mukund P Tantak, Dalip Kumar, Atish T Paul
A series of twenty four 2-(carbazol-3-yl)-2-oxoacetamide analogues were synthesized, characterized and evaluated for their pancreatic lipase (PL) inhibitory activity. Porcine PL was used against 4-nitrophenyl butyrate (method A) and tributyrin (methods B and C) as substrates during the PL inhibition assay. Compounds 7e, 7f and 7p exhibited potential PL inhibitory activity (IC50 values of 6.31, 8.72 and 9.58μM, respectively in method A; and Xi50 of 21.85, 21.94 and 26.2, respectively in method B). Further, inhibition kinetics of 7e, 7f and 7p against PL, using method A, revealed their competitive nature of inhibition...
January 15, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/27885194/isolation-and-characterization-of-a-thermostable-lipase-from-bacillus-thermoamylovorans-nb501
#7
Chihaya Yamada, Kota Sawano, Noriyasu Iwase, Masaki Matsuoka, Takatoshi Arakawa, Shigeo Nishida, Shinya Fushinobu
Two thermophilic bacterial strains, Bacillus thermoamylovorans NB501 and NB502, were isolated from a high-temperature aerobic fermentation reactor system that processes tofu refuse (okara) in the presence of used soybean oil. We cloned a lipase gene from strain NB501, which secretes a thermophilic lipase. The biochemical characteristics of the recombinant enzyme (Lip501r) were elucidated. Lip501r is monomeric in solution with an apparent molecular mass of 38 kDa on SDS-PAGE. The optimal pH and apparent optimal temperature of Lip501r were 8 and 60°C, respectively...
November 22, 2016: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/27871377/enhancement-of-methanol-resistance-of-yarrowia-lipolytica-lipase-2-using-%C3%AE-cyclodextrin-as-an-additive-insights-from-experiments-and-molecular-dynamics-simulation
#8
Hao Cao, Yang Jiang, Haiyang Zhang, Kaili Nie, Ming Lei, Li Deng, Fang Wang, Tianwei Tan
The methanol resistance of lipase is a critical parameter in enzymatic biodiesel production. In the present work, the methanol resistance of Yarrowia lipolytica Lipase 2 (YLLIP2) was significantly improved using β-cyclodextrin (β-CD) as an additive. According to the results, YLLIP2 with β-CD exhibited approximately 7000U/mg specific activity in 30wt% methanol for 60min compared with no activity without β-CD under the same conditions. Molecular dynamics (MD) simulation results indicated that the β-CD molecules weakened the conformational change of YLLIP2 and maintained a semi-open state of the lid by overcoming the interference caused by methanol molecules...
January 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/27821336/a-comparative-study-on-kinetics-and-substrate-specificities-of-phospholipase-a1-with-thermomyces-lanuginosus-lipase
#9
Ruipu Xin, Faez Iqbal Khan, Zexin Zhao, Zedong Zhang, Bo Yang, Yonghua Wang
The mechanism of lipase binding to the lipid-water interface is crucial for substrate specificity and kinetic properties. In this study, the chain-length specificity, regiospecificity and substrate specificity of Phospholipase A1 (PLA1) and its parent enzyme Thermomyces lanuginosus lipase (TLL) have been investigated using a classical emulsion system. The results show that both PLA1 and TLL are 1,3-regioselective lipases. Additionally, the hydrolytic activity of PLA1 is comparatively lower on short-chain triacylglyceride (TAG) and higher on phosphatidylcholine (PC) than the hydrolytic activity of TLL...
February 15, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/27779408/molecular-mechanism-behind-solvent-concentration-dependent-optimal-activity-of-thermomyces-lanuginosus-lipase-in-a-biocompatible-ionic-liquid-interfacial-activation-through-arginine-switch
#10
Sudip Das, Tarak Karmakar, Sundaram Balasubramanian
Thermomyces lanuginosus lipase (TLL) is an industrially significant catalyst for the production of biodiesel due to its operability over a wide range of pH's and temperatures. Molecular dynamics simulations of TLL in aqueous solutions of a biocompatible ionic liquid (IL), cholinium glycinate (ChGly), have been carried out to investigate the microscopic reasons for the experimentally observed enhancement in the activity of TLL upon addition of a room temperature IL (RTIL), especially at an optimal concentration...
November 17, 2016: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/27693248/controlled-lid-opening-in-thermomyces-lanuginosus-lipase-an-engineered-switch-for-studying-lipase-function
#11
Jakob Skjold-Jørgensen, Jesper Vind, Olga V Moroz, Elena Blagova, Vikram K Bhatia, Allan Svendsen, Keith S Wilson, Morten J Bjerrum
Here, we present a lipase mutant containing a biochemical switch allowing a controlled opening and closing of the lid independent of the environment. The closed form of the TlL mutant shows low binding to hydrophobic surfaces compared to the binding observed after activating the controlled switch inducing lid-opening. We directly show that lipid binding of this mutant is connected to an open lid conformation demonstrating the impact of the exposed amino acid residues and their participation in binding at the water-lipid interface...
September 28, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27529468/deciphering-the-toxicity-of-bisphenol-a-to-candida-rugosa-lipase-through-spectrophotometric-methods
#12
Rui Zhang, Lining Zhao, Rutao Liu
Bisphenol A is widely used in the manufacture of food packaging and beverage containers and can invade our food and cause contamination. Candida rugose lipase has been a versatile enzyme for biocatalysis and biotransformations to produce useful materials for food, pharmaceutical and flavor. The interactions between bisphenol A and Candida rugosa lipase in vitro were studied by UV-vis, steady-state fluorescence, circular dichroism, synchronous fluorescence, light scattering spectra, molecular docking and enzyme activity assay to better understand the toxicity and toxic mechanisms of bisphenol A...
October 2016: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/27499063/free-energy-studies-reveal-a-possible-mechanism-for-oxidation-dependent-inhibition-of-mgl
#13
Laura Scalvini, Federica Vacondio, Michele Bassi, Daniele Pala, Alessio Lodola, Silvia Rivara, Kwang-Mook Jung, Daniele Piomelli, Marco Mor
The function of monoacylglycerol lipase (MGL), a key actor in the hydrolytic deactivation of the endocannabinoid 2-arachidonoyl-sn-glycerol (2AG), is tightly controlled by the cell's redox state: oxidative signals such as hydrogen peroxide suppress MGL activity in a reversible manner through sulfenylation of the peroxidatic cysteines, C201 and C208. Here, using as a starting point the crystal structures of human MGL (hMGL), we present evidence from molecular dynamics and metadynamics simulations along with high-resolution mass spectrometry studies indicating that sulfenylation of C201 and C208 alters the conformational equilibrium of the membrane-associated lid domain of MGL to favour closed conformations of the enzyme that do not permit the entry of substrate into the active site...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27474867/toluene-promotes-lid-2-interfacial-activation-of-cold-active-solvent-tolerant-lipase-from-pseudomonas-fluorescens-strain-ams8
#14
Norhayati Yaacob, Mohd Shukuri Mohamad Ali, Abu Bakar Salleh, Raja Noor Zaliha Raja Abdul Rahman, Adam Thean Chor Leow
The utilization of cold active lipases in organic solvents proves an excellent approach for chiral synthesis and modification of fats and oil due to the inherent flexibility of lipases under low water conditions. In order to verify whether this lipase can function as a valuable synthetic catalyst, the mechanism concerning activation of the lid and interacting solvent residues in the presence of organic solvent must be well understood. A new alkaline cold-adapted lipase, AMS8, from Pseudomonas fluorescens was studied for its structural adaptation and flexibility prior to its exposure to non-polar, polar aprotic and protic solvents...
July 2016: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/27419053/lid-mobility-in-lipase-smg1-validated-using-a-thiol-disulfide-redox-potential-probe
#15
Shaohua Guo, Grzegorz Maria Popowicz, Daoming Li, Dongjuan Yuan, Yonghua Wang
Most lipases possess a lid domain above the catalytic site that is responsible for their activation. Lipase SMG1 from Malassezia globose CBS 7966 (Malassezia globosa LIP1), is a mono- and diacylglycerol lipase with an atypical loop-like lid domain. Activation of SMG1 was proposed to be solely through a gating mechanism involving two residues (F278 and N102). However, through disulfide bond cross-linking of the lid, this study shows that full activation also requires mobility of the lid domain, contrary to a previous proposal...
May 2016: FEBS Open Bio
https://www.readbyqxmd.com/read/27155582/lid-closure-dynamics-of-porcine-pancreatic-lipase-in-aqueous-solution
#16
Neshatul Haque, N Prakash Prabhu
BACKGROUND: Pancreatic lipases hydrolyze fatty acids in dietary pathway. The activity of porcine pancreatic lipase (PPL) is controlled by lid domain along with a coenzyme, colipase. The active open-state conformation of the protein could be induced by detergents or bile salts which would be further stabilized by binding of colipase. In the absence of these interactions, the lid preferably attains a closed conformation in water. METHODS: Molecular dynamic simulation was used to monitor the lid movement of PPL in open and closed conformations in water...
October 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27155580/lid-dynamics-of-porcine-pancreatic-lipase-in-non-aqueous-solvents
#17
Neshatul Haque, N Prakash Prabhu
BACKGROUND: Understanding the dynamics of enzymes in organic solvents has wider implications on their industrial applications. Pancreatic lipases, which show activity in their lid open-state, demonstrate enhanced activity in organic solvents at higher temperatures. However, the lid dynamics of pancreatic lipases in non-aqueous environment is yet to be clearly understood. METHODS: Dynamics of porcine pancreatic lipase (PPL) in open and closed conformations was followed in ethanol, toluene, and octanol using molecular simulation methods...
October 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27012424/structural-basis-of-lipid-targeting-and-destruction-by-the-type-v-secretion-system-of-pseudomonas-aeruginosa
#18
Paulo Vinicius da Mata Madeira, Samira Zouhir, Pauline Basso, David Neves, Aurélie Laubier, Richard Salacha, Sophie Bleves, Eric Faudry, Carlos Contreras-Martel, Andréa Dessen
The type V secretion system is a macromolecular machine employed by a number of bacteria to secrete virulence factors into the environment. The human pathogen Pseudomonas aeruginosa employs the newly described type Vd secretion system to secrete a soluble variant of PlpD, a lipase of the patatin-like family synthesized as a single macromolecule that also carries a polypeptide transport-associated domain and a 16-stranded β-barrel. Here we report the crystal structure of the secreted form of PlpD in its biologically active state...
May 8, 2016: Journal of Molecular Biology
https://www.readbyqxmd.com/read/26934700/analysis-of-comparative-sequence-and-genomic-data-to-verify-phylogenetic-relationship-and-explore-a-new-subfamily-of-bacterial-lipases
#19
Malihe Masomian, Raja Noor Zaliha Raja Abd Rahman, Abu Bakar Salleh, Mahiran Basri
Thermostable and organic solvent-tolerant enzymes have significant potential in a wide range of synthetic reactions in industry due to their inherent stability at high temperatures and their ability to endure harsh organic solvents. In this study, a novel gene encoding a true lipase was isolated by construction of a genomic DNA library of thermophilic Aneurinibacillus thermoaerophilus strain HZ into Escherichia coli plasmid vector. Sequence analysis revealed that HZ lipase had 62% identity to putative lipase from Bacillus pseudomycoides...
2016: PloS One
https://www.readbyqxmd.com/read/26930469/selective-disruption-of-disulphide-bonds-lowered-activation-energy-and-improved-catalytic-efficiency-in-talipb-from-trichosporon-asahii-msr54-md-simulations-revealed-flexible-lid-and-extended-substrate-binding-area-in-the-mutant
#20
Yogesh Singh, Namita Gupta, Ved Vrat Verma, Manisha Goel, Rani Gupta
TALipB (33 kDa) is a solvent stable, enantioselective lipase from Trichosporon asahii MSR54. It is cysteine-rich and shows activation in presence of thiol reducing agents. DIANNA server predicted three disulphide bridges C53-C195 (S1), C89-C228 (S2) and C164-C254 (S3) in the enzyme. Selective disruption of disulphide bonds by cysteine to alanine mutations at Cys53 and Cys89 of S1 and S2 bonds resulted in enzyme activation. Mutant mTALipB (S1+S2) showed increase in specific activity by ∼4-fold (834 mM/mg) and improved Vmax of 6...
March 25, 2016: Biochemical and Biophysical Research Communications
keyword
keyword
76160
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"