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https://www.readbyqxmd.com/read/29684853/naringenin-improve-hepatitis-c-virus-infection-induced-insulin-resistance-by-increase-pten-expression-via-p53-dependent-manner
#1
Benli Jia, Dongsheng Yu, Gang Yu, Yunsheng Cheng, Yang Wang, Xiaoqiang Yi, Xiaoping Li, Yong Wang
Hepatitis C virus (HCV) infection may finally lead to hepatocellular carcinoma (HCC), and also associated with insulin resistance (IR). Naringenin (NGEN), a flavonoid found in grapefruit, has anti-virus, anti-inflammation and insulin sensitization effects. In the present study we examined the effects of NGEN on HCV core protein (HCVCP) infection induced IR and investigated the mechanism involved. We found that NGEN ameliorated IR and glucose tolerance in HCVCP infected mice by increase the phosphorylation of Akt at both Ser473 and Thr308 site, and also inhibited the inflammation cytokine production and T-cell immune response...
April 20, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29684847/the-association-between-p53-protein-phosphorylation-at-serine-15-serine-20-and-sensitivity-of-cells-isolated-from-patients-with-ovarian-cancer-and-cell-lines-to-chemotherapy-in-in-vitro-study
#2
Iwona Kamińska, Julia K Bar
BACKGROUND: The association between p53 protein phosphorylated at serine 15 (Ser15), serine 20 (Ser20) and ovarian tumor cell sensitivity after chemotherapy was analyzed in order to define the influence of p53 activation on tumor cell sensitivity to chemotherapy. METHODS: The study was performed on ovarian cancer cell line (OvBH-1), colon adenocarcinoma metastasis to ovary (SW626) and on cells isolated from ascitic fluids from patients with ovarian cancer: with (p53+) or without (p53-) p53 nuclear protein accumulation...
December 15, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/29684791/the-role-of-promyelocytic-leukemia-protein-in-steatosis-associated-hepatic-tumors-related-to-chronic-hepatitis-b-virus-infection
#3
Yih-Lin Chung, Mei-Ling Wu
The persistence of hepatitis B surface antigen (HBsAg) is a risk factor for the development of steatosis-associated tumors in chronic hepatitis B virus (HBV) infection, yet little is known about the metabolic link with this factor. We correlated HBV-related pathogenesis in genetically engineered mice and human carriers with metabolic proteomics and lipogenic gene expression profiles. The immunohistochemistry showed that the promyelocytic leukemia protein (PML, a tumor suppressor involved in genome maintenance and fatty acid oxidation), being inversely influenced by the dynamic HBsAg levels from acute phase to seroclearance, appeared as a lipo-metabolic switch linking HBsAg-induced steatosis (lipogenesis) to HBsAg-lost fat-burning hepatocarcinogenesis (lipolysis)...
April 20, 2018: Translational Oncology
https://www.readbyqxmd.com/read/29684757/microrna-144-suppresses-the-expression-of-cytokines-through-targeting-rankl-in-the-matured-immune-cells
#4
Rui-Dong Li, Cong-Huan Shen, Yi-Feng Tao, Xiao-Fei Zhang, Quan-Bao Zhang, Zhen-Yu Ma, Zheng-Xin Wang
To investigate whether the microRNA-144 (miR-144) had immune regulation effect on matured immune cells, we firstly used quantitative RT-PCR (qRT-PCR) to detect the expression changes of miR-144 between the matured and immature dendritic cells (DCs), macrophages, and the peripheral blood mononuclear cells (PBMCs). Then we went on inspecting the expression changes of TNF-α, IL-1β, IL-6 and IL-23 in the matured DCs treated with miR-144 mimics or inhibitors using qRT-PCR, and also performed western blot to test phosphorylation state of ERK, JNK, p38 and p65 in these cells...
April 20, 2018: Cytokine
https://www.readbyqxmd.com/read/29684755/impaired-tgf-%C3%AE-signaling-in-patients-with-active-systemic-lupus-erythematosus-is-associated-with-an-overexpression-of-il-22
#5
Raja Rekik, Monia Smiti Khanfir, Thara Larbi, Imen Zamali, Asma Beldi-Ferchiou, Ons Kammoun, Soumaya Marzouki, Saloua Hamzaoui, Skander Mrad, Mohamed Ridha Barbouche, Mohamed Habib Houman, Melika Ben Ahmed
The mechanisms leading to the disruption of self-tolerance in systemic lupus erythematosus (SLE) remain elusive. Herein, we aimed to decipher the molecular basis of the impaired response of mononuclear cells to TGF-β1. The Smad3-pathway was explored on CD3+ lymphocytes in either active or non active SLE patients. An impaired transcription of TGF-β1 target genes was demonstrated in the CD3+ lymphocytes of active SLE patients confirming that the defect involves T cells and pointing to its extrinsic nature. We further demonstrate that the defect did not result from an impaired TGF-βRII expression or Smad2/3 phosphorylation suggesting that the mechanism lies downstream Smad2/3 translocation...
April 20, 2018: Cytokine
https://www.readbyqxmd.com/read/29684683/brain-related-proteins-as-potential-csf-biomarkers-of-alzheimer-s-disease-a-targeted-mass-spectrometry-approach
#6
Ilijana Begcevic, Davor Brinc, Marshall Brown, Eduardo Martinez-Morillo, Oliver Goldhardt, Timo Grimmer, Viktor Magdolen, Ihor Batruch, Eleftherios P Diamandis
Alzheimer's disease (AD) is the most common cause of dementia, characterized by progressive cognitive decline. The main disease hallmarks include amyloid beta aggregates and neurofibrillary tangles. Brain pathology is reflected in cerebrospinal fluid (CSF); the core biomarkers amyloid beta 1-42, total and phosphorylated tau protein levels are changed, relative to cognitively normal elderly. Still, there is a need for additional biomarkers which could identify disease more accurately and at an earlier stage, predict severity and be used in research settings...
April 20, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29684538/molecular-cloning-characterization-and-expression-analysis-of-c-ebp-%C3%AE-%C3%AE-and-%C3%AE-in-adipose-related-tissues-and-adipocyte-of-duck-anas-platyrhynchos
#7
Jiamin Qiu, Wanxia Wang, Shenqiang Hu, Yushi Wang, Wenqiang Sun, Jiwei Hu, Jiwen Wang
CCAAT/enhancer binding protein α, β, δ (C/EBP α, β, δ) are essential transcriptional factors in regulating adipose development. However, information about their sequence characteristics and functions during adipocyte development still remains scarce in birds. In present study, we found that duck C/EBP α, β, δ differed in their phosphorylation sites and low complexity regions (LCRs) among their orthologs and paralogs. Phylogenetic analysis showed that C/EBP α, β, δ had different evolutionary patterns, and each of duck C/EBP α, β, δ was strikingly diverged from orthologs of other Aves...
April 20, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29684490/phosphorylated-nucleolar-tau-protein-is-related-to-the-neuronal-in-vitro-differentiation
#8
Concetta Federico, Laura Gil, Francesca Bruno, Agata Grazia D'Amico, Velia D'Agata, Salvatore Saccone
Tau is a multifunctional protein, originally identified as a cytoplasmic protein associated with microtubules. It is codified by the MAPT gene, and the alternative splicing, in the neuronal cells, results in six different isoforms. Tau was subsequently observed in the cell nucleus, where its function is not yet clearly understood. Here, we studied the MAPT gene and the cellular localization of the AT8 and Tau-1 epitopes of Tau protein, in the SK-N-BE cell line, which differentiates in neuronal-like cells after retinoic acid treatment...
April 20, 2018: Gene
https://www.readbyqxmd.com/read/29684479/impairment-of-the-gn%C3%AE-11-controlled-expression-of-claudin-1-and-mmp-9-and-collective-migration-of-human-breast-cancer-mcf-7-cells-by-dheas
#9
Neha Upmanyu, Ahmed Bulldan, Dimitrios Papadopoulos, Raimund Dietze, Viveka Nand Malviya, Georgios Scheiner-Bobis
Although dehydroepiandrosterone sulfate (DHEAS) constitutes the most abundant steroid in humans, in-depth investigations of its effects are rather scarce. We address here DHEAS effects on the estrogen receptor-positive metastatic human breast cancer cell line MCF-7. We focus on DHEAS-mediated signaling that might influence expression of claudin-1 and matrix metalloproteinase-9 (MMP-9), both known to be critical factors for migration and invasiveness of various cancers, including breast cancer cells. Physiological concentrations of DHEAS trigger persistent phosphorylation of Erk1/2 in MCF-7 cells...
April 20, 2018: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29684334/the-mkk7-inhibitor-peptide-gadd45%C3%AE-i-attenuates-er-stress-induced-mitochondrial-dysfunction-in-ht22-cells-involvement-of-jnk-wnt-pathway
#10
Guang-Hua Xu, Bing-Jun Song, Dan Liu, Yu-Hua Chen, Yuan Zhou, Wen-Bo Liu, Hua Li, Tian-Lin Long, Rui Zhang, Wei Liu
JNK, a member of the mitogen activated protein kinases (MAPKs) superfamily, plays a key role in cell death in many neurological disorders, but systemic inhibition of JNK has detrimental side effects. JNK can be regulated by two direct upstream kinases: MAPK kinase 4 (MKK4) and MAPK kinase 7 (MKK7). Here, we investigated the effect of GADD45β-I, a recently designed cell-permeable inhibitor peptide for MKK7, on endoplasmic reticulum (ER) stress-induced cytotoxicity in neuronal HT22 cells. We found that treatment with the ER stress inducer tunicamycin (TM) increased the phosphorylation of JNK and MKK7 in HT22 cells, which was nullified by GADD45β-I...
April 20, 2018: Brain Research
https://www.readbyqxmd.com/read/29684333/effects-of-alterations-of-the-e-coli-lipopolysaccharide-layer-on-membrane-permeabilization-events-induced-by-cecropin-a
#11
Anurag Agrawal, James C Weisshaar
The outermost layer of Gram negative bacteria is composed of a lipopolysaccharide (LPS) network that forms a dense protective hydrophilic barrier against entry of hydrophobic drugs. At low μM concentrations, a large family of cationic polypeptides known as antimicrobial peptides (AMPs) is able to penetrate the LPS layer and permeabilize the outer membrane (OM) and the cytoplasmic membrane (CM), causing cell death. Cecropin A is a well-studied cationic AMP from moth. Here a battery of time-resolved, single-cell microscopy experiments explores how deletion of sugar layers and/or phosphoryl negative charges from the core oligosaccharide layer (core OS) of K12 E...
April 20, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29684324/taxonomic-distribution-structure-function-relationship-and-metabolic-context-of-the-two-families-of-sulfide-dehydrogenases-sqr-and-fcsd
#12
Filipe M Sousa, Juliana G Pereira, Bruno C Marreiros, Manuela M Pereira
Hydrogen sulfide (H2 S) is a versatile molecule with different functions in living organisms: it can work as a metabolite of sulfur and energetic metabolism or as a signaling molecule in higher Eukaryotes. H2 S is also highly toxic since it is able to inhibit heme cooper oxygen reductases, preventing oxidative phosphorylation. Due to the fact that it can both inhibit and feed the respiratory chain, the immediate role of H2 S on energy metabolism crucially relies on its bioavailability, meaning that studying the central players involved in the H2 S homeostasis is key for understanding sulfide metabolism...
April 20, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29684271/protein-semisynthesis-provides-access-to-tau-disease-associated-post-translational-modifications-ptms-and-paves-the-way-to-deciphering-the-tau-ptm-code-in-health-and-diseased-states
#13
Mahmood Haj-Yahya, Hilal A Lashuel
The microtubule-associated protein Tau plays a central role in neurodegeneration and is a leading therapeutic target for the treatment of Alzheimer's disease (AD). Several lines of evidence suggest that post-translational modifications (PTMs) regulate the function(s) of Tau, including its subcellular localization, clearance, aggregation, toxicity, and pathology spreading. However, the lack of tools and methodologies that allow site-specific introduction of PTMs in Tau have limited our ability to dissect the role of PTMs in regulating Tau functions in health and disease...
April 23, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29684168/emerin-modulates-spatial-organization-of-chromosome-territories-in-cells-on-softer-matrices
#14
Roopali Pradhan, Devika Ranade, Kundan Sengupta
Cells perceive and relay external mechanical forces into the nucleus through the nuclear envelope. Here we examined the effect of lowering substrate stiffness as a paradigm to address the impact of altered mechanical forces on nuclear structure-function relationships. RNA sequencing of cells on softer matrices revealed significant transcriptional imbalances, predominantly in chromatin associated processes and transcriptional deregulation of human Chromosome 1. Furthermore, 3-Dimensional fluorescence in situ hybridization (3D-FISH) analyses showed a significant mislocalization of Chromosome 1 and 19 Territories (CT) into the nuclear interior, consistent with their transcriptional deregulation...
April 19, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29684161/impact-of-heat-stress-during-the-follicular-phase-on-porcine-ovarian-steroidogenic-and-phosphatidylinositol-3-signaling
#15
M J Dickson, C L Hager, A Al-Shaibi, P Q Thomas, L H Baumgard, J W Ross, A F Keating
Environmental conditions that impede heat dissipation and increase body temperature cause heat stress (HS). The study objective was to evaluate impacts of HS on the follicular phase of the estrous cycle. Post-pubertal gilts (126.0 ± 21.6 kg) were orally administered altrenogest to synchronize estrus, and subjected to either 5 d of thermal neutral (TN; 20.3 ± 0.5°C; n = 6) or cyclical HS (25.4 - 31.9°C; n = 6) conditions during the follicular phase preceding behavioral estrus. On d 5, blood samples were obtained, gilts were euthanized, and ovaries collected...
April 19, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29684114/exploring-genetic-outcomes-as-frailty-biomarkers
#16
Vanessa Valdiglesias, María Sánchez-Flores, Diego Marcos-Pérez, Laura Lorenzo-López, Ana Maseda, José C Millán-Calenti, Eduardo Pásaro, Blanca Laffon
Frailty has emerged as a reliable measure of the aging process. Since the early detection of frailty is crucial to prevent or even revert it, the use of biomarkers would allow an earlier and more objective identification of frail individuals. In order to improve the understanding of the biological features associated with frailty as well as to explore different biomarkers for its early identification, several genetic outcomes - mutagenicity, different types of genetic damage, and cellular repair capacity - were analysed in a population of older adults classified into frail, pre-frail and non-frail...
April 19, 2018: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
https://www.readbyqxmd.com/read/29684069/autophosphorylation-of-ser-6-via-an-intermolecular-mechanism-is-important-for-the-rapid-reduction-of-ntcdpk1-kinase-activity-for-substrate-rsg
#17
Takeshi Ito, Sarahmi Ishida, Yohsuke Takahashi
Tobacco (Nicotiana tabacum) Ca2+-dependent protein kinase 1 (NtCDPK1) is involved in feedback regulation of the plant hormone gibberellin through the phosphorylation of the transcription factor, REPRESSION OF SHOOT GROWTH (RSG). Previously, Ser-6 and Thr-21 were identified as autophosphorylation sites in NtCDPK1. Autophosphorylation of Ser-6 and Thr-21 not only decreases the binding affinity of NtCDPK1 for RSG, but also inhibits the homodimerization of NtCDPK1. Furthermore, autophosphorylation decreases the phosphorylation efficiency of RSG...
2018: PloS One
https://www.readbyqxmd.com/read/29683976/activating-p2x7-receptors-increases-proliferation-of-human-pancreatic-cancer-cells-via-erk1-2-and-jnk
#18
Ji Hun Choi, Young Geon Ji, Jung Jae Ko, Han Jun Cho, Dong Hyeon Lee
OBJECTIVES: The aim of this study was to investigate the effects of the activated P2X7 receptors on the proliferation and growth of human pancreatic cancer cells. METHODS: Proliferation was measured by incorporating bromodeoxyuridine into pancreatic cancer cells, MIA PaCa-2 and HPAC. Expression of P2 receptors and signal molecules was examined using quantitative reverse transcription/polymerase chain reaction and/or Western blot. Proliferative effects of the P2X7 receptors in vivo were examined using a xenotransplant model of pancreatic cancer cell lines...
April 19, 2018: Pancreas
https://www.readbyqxmd.com/read/29683756/de-novo-expression-of-transfected-sirtuin-3-enhances-susceptibility-of-human-mcf-7-breast-cancer-cells-to-hyperoxia-treatment
#19
Marija Pinterić, Iva I Podgorski, Sandra Sobočanec, Marijana Popović Hadžija, Mladen Paradžik, Ana Dekanić, Maja Marinović, Mirna Halasz, Robert Belužić, Grazia Davidović, Andreja Ambriović Ristov, Tihomir Balog
Sirtuin 3 (Sirt3) has a promising role in cancer tumourigenesis and treatment, but there have been controversies about its role as oncogene or tumour suppressor in different types of cancer. Changes in its expression are associated with the excessive production of reactive oxygen species (ROS), thus contributing to mitochondrial dysfunction and age-related pathologies. Hyperoxic treatment (i.e. generator of ROS) was shown to support some tumourigenic properties, but finally suppresses growth of certain mammary carcinoma cells...
April 23, 2018: Free Radical Research
https://www.readbyqxmd.com/read/29683733/cep55-overexpression-causes-male-specific-sterility-in-mice-by-suppressing-foxo1-nuclear-retention-through-sustained-activation-of-pi3k-akt-signaling
#20
Debottam Sinha, Murugan Kalimutho, Josephine Bowles, Ai-Leen Chan, D Jo Merriner, Amanda L Bain, Jacinta L Simmons, Raimundo Freire, J Alejandro Lopez, Robin M Hobbs, Moira K O'Bryan, Kum Kum Khanna
Spermatogenesis is a dynamic process involving self-renewal and differentiation of spermatogonial stem cells, meiosis, and ultimately, the differentiation of haploid spermatids into sperm. Centrosomal protein (CEP)-55 is necessary for somatic cell abscission during cytokinesis. It facilitates equal segregation of cytoplasmic contents between daughter cells by recruiting endosomal sorting complex required for transport machinery (ESCRT) at the midbody. In germ cells, CEP55, in partnership with testes expressed-14 protein (TEX14), has also been shown to be an integral component of intercellular bridge before meiosis...
April 17, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
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