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https://www.readbyqxmd.com/read/28110249/design-an-aptasensor-based-on-structure-switching-aptamer-on-dendritic-gold-nanostructures-fe3o4-sio2-dabco-modified-screen-printed-electrode-for-highly-selective-detection-of-epirubicin
#1
Ayemeh Bagheri Hashkavayi, Jahan Bakhsh Raoof
The present work describes a label free electrochemical aptasensor for selective detection of epirubicin. In this project, 5'-thiole terminated aptamer was self-assembled on carbon screen printed electrode, which modified with electrodeposited gold nanoparticles on magnetic double-charged diazoniabicyclo [2.2.2] octane dichloride silica hybrid (Fe3O4@SiO2/DABCO) by Au-S bond. The interactions of epirubicin with aptamer on the AuNPs/Fe3O4@SiO2/DABCO/SPE have been studied by cyclic voltammetry, linear sweep voltammetry and electrochemical impedance spectroscopy...
January 14, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28110248/regulation-of-human-norovirus-vpg-nucleotidylylation-by-propol-and-nucleoside-triphosphate-binding-by-its-amino-terminal-sequence-in-vitro
#2
Alexei Medvedev, Prasanth Viswanathan, Jared May, Brent Korba
The VPg protein of human Norovirus (hNoV) is a multi-functional protein essential for virus replication. The un-cleaved viral precursor protein, ProPol (NS5-6) was 100-fold more efficient in catalyzing VPg nucleotidylylation than the mature polymerase (Pol, NS6), suggesting a specific intracellular role for ProPol. Sequential and single-point alanine substitutions revealed that several positively charged amino acids in the N-terminal region of VPg regulate its nucleotidylylation by ProPol. We provide evidence that VPg directly binds NTPs, inhibition of binding inhibits nucleotidylylation, and NTP binding appears to involve the first 13 amino acids of the protein...
January 19, 2017: Virology
https://www.readbyqxmd.com/read/28110208/inflammatory-pathway-genes-associated-with-inter-individual-variability-in-the-trajectories-of-morning-and-evening-fatigue-in-patients-receiving-chemotherapy
#3
Fay Wright, Marilyn Hammer, Steven M Paul, Bradley E Aouizerat, Kord M Kober, Yvette P Conley, Bruce A Cooper, Laura B Dunn, Jon D Levine, Gail DEramo Melkus, Christine Miaskowski
Fatigue, a highly prevalent and distressing symptom during chemotherapy (CTX), demonstrates diurnal and interindividual variability in severity. Little is known about the associations between variations in genes involved in inflammatory processes and morning and evening fatigue severity during CTX. The purposes of this study, in a sample of oncology patients (N=543) with breast, gastrointestinal (GI), gynecological (GYN), or lung cancer who received two cycles of CTX, were to determine whether variations in genes involved in inflammatory processes were associated with inter-individual variability in initial levels as well as in the trajectories of morning and evening fatigue...
January 19, 2017: Cytokine
https://www.readbyqxmd.com/read/28110092/cutin-synthase2-maintains-progressively-developing-cuticular-ridges-in-arabidopsis-sepals
#4
Lilan Hong, Joel Brown, Nicholas A Segerson, Jocelyn K C Rose, Adrienne H K Roeder
The cuticle is a crucial barrier on the aerial surfaces of land plants. In many plants, including Arabidopsis, the sepals and petals form distinctive nanoridges in their cuticles. Yet little is known about how the formation and maintenance of these nanostructures is coordinated with the growth and development of the underlying cells. Here we characterize the cutin synthase 2 (cus2) mutant, which causes a great reduction in cuticular ridges on the mature sepal epidermis, but only a moderate effect on petal cone cell ridges...
January 18, 2017: Molecular Plant
https://www.readbyqxmd.com/read/28110064/activation-of-dendritic-cells-by-low-molecular-weight-oyster-polysaccharides
#5
Ming Zhong, Cheng Zhong, Tingting Wang, Pei Hu, Guanghui Wang, Rujing Ren, Jing Zhang, Huijei Gao, Wen Cui, Wenjuan Duan, Jiantu Che
Dendritic cells play a primary role in antigen presentation to CD4(+) T cells, which initiate acquired immune responses. Therefore, determining positive modulators of dendritic cell activation to improve therapeutic approaches for cancer treatment might be useful. We here investigated the effects of low molecular weight oyster polysaccharides (LMW-OPS) on bone marrow-derived dendritic cells (BMDCs) obtained from mice. LMW-OPS increased the surface expression of major histocompatibility complex class II (MHC-II), CD40 and CD86 in BMDCs and induced the secretion of tumour necrosis factor (TNF)-α and interleukin (IL)-12, which were significantly decreased in the BMDCs derived from MyD88(-/-) mice but not from the lipopolysaccharide-resistant C3H/HeJ mice...
January 18, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28110028/surgical-ethical-and-psychosocial-considerations-in-head-transplantation
#6
REVIEW
Allen Furr, Mark A Hardy, Juan P Barret, John H Barker
Transplanting a head and brain is perhaps the final frontier of organ transplantation. The goal of body-to-head transplantation (BHT) is to sustain the life of individuals who suffer from terminal disease, but whose head and brain are healthy. Ideally BHT could provide a lifesaving treatment for several conditions where none currently exists. BHT is no ordinary experiment, to transfer a head to another body involves extraordinarily complex medical challenges as well as ethical and existential dilemmas that were previously confined to the imagination of writers of fiction...
January 18, 2017: International Journal of Surgery
https://www.readbyqxmd.com/read/28109950/coumarin-derivatives-as-potential-antitumor-agents-growth-inhibition-apoptosis-induction-and-multidrug-resistance-reverting-activity
#7
Alessandra Bisi, Concettina Cappadone, Angela Rampa, Giovanna Farruggia, Azzurra Sargenti, Federica Belluti, Rita M C Di Martino, Emil Malucelli, Alessia Meluzzi, Stefano Iotti, Silvia Gobbi
A small library of coumarins, carrying butynyl-amino chains, was synthesized continuing our studies in the field of MDR reverting ageEnts and in order to obtain multipotent agents to combat malignancies. In particular, the reported anticancer and chemopreventive natural product 7-isopentenyloxycoumarin was linked to different terminal amines, selected on the basis of our previously reported results. The anticancer behaviour and the MDR reverting ability of the new compounds were evaluated on human colon cancer cells, particularly prone to develop the MDR phenotype...
January 13, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28109905/cloning-genetic-engineering-and-characterization-of-tmof-expressed-in-saccharomyces-cerevisiae-to-control-larval-mosquitoes
#8
Borovsky Dov, Sabine Nauewelaers, Charles A Powell, Robert G Shatters
Trypsin modulating oostatic factor, a decapaptide isolated from the ovaries of A. aegypti, is the physiological factor that terminates the trypsin biosynthesis after the blood meal. Earlier results obtained from feeding mosquito larvae and injecting female mosquitoes with TMOF show that trypsin biosynthesis and egg development are inhibited, indicating that TMOF traverses the gut epithelial cells and modulates trypsin biosynthesis, making it a potential larvacidal peptide hormone. Therefore, TMOF and TMOF green fluorescent protein (GFP) fusion protein with a trypsin cleavage site, allowing TMOF release in the larval gut, were expressed in S...
January 18, 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/28109894/pregnancy-outcomes-following-direct-uterine-fibroid-thermal-ablation-a-review-of-the-literature
#9
REVIEW
Julia Keltz, Mark Levie, Scott Chudnoff
The objective of this review is to describe the reproductive outcomes of women after radiofrequency volumetric thermal ablation (RFVTA) of fibroids or magnetic resonance-guided high-intensity focused ultrasound (MRgHIFU). This is a literature review of the current case reports of reproductive outcomes following direct fibroid thermal ablation at multiple academic and private centers throughout the world. A literature search was performed using PubMed and Medline. All publications that included data of women who underwent radiofrequency fibroid ablation or magnetic resonance-guided high-intensity focused ultrasound of fibroids and subsequently conceived were included...
January 18, 2017: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/28109886/truncated-prion-protein-prp226-a-structural-view-on-its-role-in-amyloid-disease
#10
Valerija Kovač, Blaž Zupančič, Gregor Ilc, Janez Plavec, Vladka Čurin Šerbec
In the brain of patients with transmissible spongiform encephalopathies, besides PrP(Sc) aggregates, deposition of truncated PrP molecules was described. Jansen et al. reported two clinical cases with deposition of C-terminally truncated PrP, one of them ending with Tyr226. We have previously described the discovery of monoclonal antibody V5B2 that selectively recognizes this version of the prion protein, which we called PrP226*. Using monoclonal antibody V5B2 we showed that accumulation of PrP226* is characteristic for most types of human and animal TSEs...
January 18, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28109855/heterologous-expression-and-characterization-of-plant-taxadiene-5%C3%AE-hydroxylase-cyp725a4-in-escherichia-coli
#11
John Edward Rouck, Bradley Walters Biggs, Amogh Kambalyal, William R Arnold, Marjan De Mey, Parayil Kumaran Ajikumar, Aditi Das
Taxadiene-5α-Hydroxylase (CYP725A4) is a membrane-bound plant cytochrome P450 that catalyzes the oxidation of taxadiene to taxadiene-5α-ol. This oxidation is a key step in the production of the valuable cancer therapeutic and natural plant product, taxol. In this work, we report the bacterial expression and purification of six different constructs of CYP725A4. All six of these constructs are N-terminally modified and three of them are fused to cytochrome P450 reductase to form a chimera construct. The construct with the highest yield of CYP725A4 protein was then selected for substrate binding and kinetic analysis...
January 18, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28109755/construction-of-agrobacterium-tumefaciens-mediated-tomato-black-ring-virus-infectious-cdna-clones
#12
Aleksandra Zarzyńska-Nowak, Inmaculada Ferriol, Bryce W Falk, Natasza Borodynko-Filas, Beata Hasiów-Jaroszewska
Tomato black ring virus (TBRV, genus Nepovirus) infects a wide range of economically important plants such as tomato, potato, tobacco and cucumber. Here, a successful construction of infectious full-length cDNA clones of the TBRV genomic RNAs (RNA1 and RNA2) is reported for the first time. The engineered constructs consisting of PCR-amplified DNAs were cloned into binary vector pJL89 immediately downstream of a double Cauliflower mosaic virus (CaMV) 35S promoter, and upstream of the Hepatitis delta virus (HDV) ribozyme and nopaline synthase terminator (NOS)...
January 18, 2017: Virus Research
https://www.readbyqxmd.com/read/28109429/body-fluid-degradomics-and-characterization-of-basic-n-terminome
#13
F Sabino, O Hermes, U Auf dem Keller
Rapid improvements in instrumentation and data analysis make mass spectrometry-based proteomics the method of choice for global characterization of proteomes and discovery of protein-based biomarkers. On the contrary to tissue biopsies, body fluids-e.g., blood, wound fluid, urine, and saliva-are noninvasive and easy to collect and process. However, they are very complex and present high dynamic ranges of protein concentrations, rendering direct shotgun proteomics analysis as inefficient for identification of low-abundance proteins in these specimens...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28109384/failing-systemic-right-ventricles-with-persistent-pulmonary-hypertension-candidates-for-ventricular-assist-devices-as-destination-therapy
#14
Ophelie Loup, Kerstin Wustmann, Michele Vittorio Martinelli, Markus Schwerzmann, Paul Mohacsi, Thierry Pierre Carrel, Alexander Kadner
Dysfunction of the systemic right ventricle is common after the atrial switch procedure for transposition of the great arteries. Cardiac transplantation remains the only long-term solution in terminal systemic right ventricular (RV) failure, but concomitant pulmonary hypertension (PHT) may preclude it. The increasing number of such patients, together with the concerns related to combined heart-lung transplantation (HLTx), urge us to consider other therapeutic options.
February 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28109316/adenosine-induced-ventricular-fibrillation-in-a-structurally-normal-heart-a-case-report
#15
Christopher A Rajkumar, Norman Qureshi, Fu Siong Ng, Vasileios F Panoulas, Phang Boon Lim
BACKGROUND: Adenosine is the first-line pharmacotherapy for termination of supraventricular tachycardia through its action on the atrioventricular node. However, pro-arrhythmic effects of adenosine are also recognised, most notably in the presence of pre-excited atrial fibrillation. In this case report, we describe the induction of ventricular fibrillation in a patient with no demonstrable accessory pathway, nor any other structural heart disease. This rare, idiosyncratic reaction has never previously been reported and is of relevance given the widespread and routine use of adenosine in clinical practice...
January 22, 2017: Journal of Medical Case Reports
https://www.readbyqxmd.com/read/28109214/simulations-of-polymer-brushes-with-charged-end-monomers-under-external-electric-fields
#16
Huanda Ding, Chao Duan, Chaohui Tong
Using Langevin dynamics simulations, the response of neutral polymer brushes with charged terminal monomers to external electric fields has been investigated. The external electric field is equivalent to the field generated by the opposite surface charges on two parallel electrodes. The effects of charge valence of terminal monomers on the structure of double layers and overall charge balance near the two electrodes were examined. Using the charge density distributions obtained from simulations, the total electric field normal to the electrodes was calculated by numerically solving the Poisson equation...
January 21, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28109212/two-structure-thermodynamics-for-the-tip4p-2005-model-of-water-covering-supercooled-and-deeply-stretched-regions
#17
John W Biddle, Rakesh S Singh, Evan M Sparano, Francesco Ricci, Miguel A González, Chantal Valeriani, José L F Abascal, Pablo G Debenedetti, Mikhail A Anisimov, Frédéric Caupin
One of the most promising frameworks for understanding the anomalies of cold and supercooled water postulates the existence of two competing, interconvertible local structures. If the non-ideality in the Gibbs energy of mixing overcomes the ideal entropy of mixing of these two structures, a liquid-liquid phase transition, terminated at a liquid-liquid critical point, is predicted. Various versions of the "two-structure equation of state" (TSEOS) based on this concept have shown remarkable agreement with both experimental data for metastable, deeply supercooled water and simulations of molecular water models...
January 21, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28109185/folding-of-intrinsically-disordered-plant-lea-proteins-is-driven-by-glycerol-induced-crowding-and-the-presence-of-membranes
#18
Anne Bremer, Martin Wolff, Anja Thalhammer, Dirk K Hincha
Late embryogenesis abundant (LEA) proteins are related to cellular dehydration tolerance. Most LEA proteins are predicted to have no stable secondary structure in solution, i.e. to be intrinsically disordered proteins (IDPs), but they may acquire α-helical structure upon drying. In the model plant Arabidopsis thaliana the LEA proteins COR15A and COR15B are highly induced upon cold treatment and are necessary for the plants to attain full freezing tolerance. Freezing leads to increased intracellular crowding due to dehydration by extracellular ice crystals...
January 21, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28109157/concerted-regulation-of-iswi-by-an-autoinhibitory-domain-and-the-h4-n-terminal-tail
#19
Johanna Ludwigsen, Sabrina Pfennig, Ashish K Singh, Christina Schindler, Nadine Harrer, Ignasi Forné, Martin Zacharias, Felix Mueller-Planitz
ISWI-family nucleosome remodeling enzymes need the histone H4 N-terminal tail to mobilize nucleosomes. Here we mapped the H4-tail binding pocket of ISWI. Surprisingly the binding site was adjacent to but not overlapping with the docking site of an auto-regulatory motif, AutoN, in the N-terminal region (NTR) of ISWI, indicating that AutoN does not act as a simple pseudosubstrate as suggested previously. Rather, AutoN cooperated with a hitherto uncharacterized motif, termed AcidicN, to confer H4-tail sensitivity and discriminate between DNA and nucleosomes...
January 21, 2017: ELife
https://www.readbyqxmd.com/read/28109119/serotonin-transporter-protects-the-placental-cells-against-apoptosis-in-caspase-3-independent-pathway
#20
Coedy Hadden, Tariq Fahmi, Anthonya Cooper, Alena V Savenka, Vladimir V Lupashin, Drucilla J Roberts, Luc Maroteaux, Sylvie Hauguel-de Mouzon, Fusun Kilic
Serotonin (5-HT) and its specific transporter, SERT play important roles in pregnancy. Using placentas dissected from 18d gestational SERT-knock out (KO), peripheral 5-HT (TPH1)-KO and wild-type (WT) mice, we explored the role of 5-HT and SERT in placental functions in detail. An abnormal thick band of fibrosis and necrosis under the giant cell layer in SERT-KO placentas appeared only moderately in TPH1-KO and minimally present in WT placentas. The majority of the changes were located at the junctional zone of the placentas in SERT...
January 21, 2017: Journal of Cellular Physiology
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