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https://www.readbyqxmd.com/read/28930702/gcamp-expression-in-retinal-ganglion-cells-characterized-using-a-low-cost-fundus-imaging-system
#1
Yao-Chuan Chang, Steven T Walston, Robert H Chow, James D Weiland
OBJECTIVE: Virus-transduced, intracellular-calcium indicators are effective reporters of neural activity, offering the advantage of cell-specific labeling. Due to the existence of an optimal time window for the expression of calcium indicators, a suitable tool for tracking GECI expression in vivo following transduction is highly desirable. APPROACH: We developed a noninvasive imaging approach based on a custom-modified, low-cost fundus viewing system that allowed us to monitor and characterize in vivo bright-field and fluorescence images of the mouse retina...
October 2017: Journal of Neural Engineering
https://www.readbyqxmd.com/read/28928803/64-slice-spiral-double-low-ct-to-evaluate-the-degree-of-stenosis-and-plaque-composition-in-diagnosing-coronary-artery-disease
#2
Xiao-Rong Feng, Li-Hua Liang, Ju-Fang Wu, Yao-Qiang Chen, Shuang Liang
This study examined the application of 64-slice spiral double-low computed tomography (CT) to evaluate the degree of coronary artery stenosis. We examined 45 patients with coronary heart disease by 64-slice spiral double-low CT and coronary angiography (CAG) to determine CT accuracy in evaluating coronary artery stenosis. Imaging analysis from 64-slice spiral double-low CT identified 199 segments with coronary stenosis from 45 patients, including 46 segments with mild stenosis, 38 with moderate stenosis and 115 with severe stenosis or artery occlusion...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28923949/cytosine-deamination-and-base-excision-repair-cause-r-loop-induced-cag-repeat-fragility-and-instability-in-saccharomyces-cerevisiae
#3
Xiaofeng A Su, Catherine H Freudenreich
CAG/CTG repeats are structure-forming repetitive DNA sequences, and expansion beyond a threshold of ∼35 CAG repeats is the cause of several human diseases. Expanded CAG repeats are prone to breakage, and repair of the breaks can cause repeat contractions and expansions. In this study, we found that cotranscriptional R-loops formed at a CAG-70 repeat inserted into a yeast chromosome. R-loops were further elevated upon deletion of yeast RNaseH genes and caused repeat fragility. A significant increase in CAG repeat contractions was also observed, consistent with previous human cell studies...
September 18, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28923333/buccal-cell-micronucleus-frequency-is-significantly-elevated-in-patients-with-spinocerebellar-ataxia-type-2
#4
Dany A Cuello-Almarales, Luis E Almaguer-Mederos, Yaimé Vázquez-Mojena, Dennis Almaguer-Gotay, Pedro Zayas-Feria, José M Laffita-Mesa, Yanetza González-Zaldívar, Raúl Aguilera-Rodríguez, Annelié Rodríguez-Estupiñán, Luis Velázquez-Pérez
Spinocerebellar ataxia type 2 (SCA2) is part of a group of at least nine dominantly inherited disorders characterized by progressive degeneration of specific neuronal populations and a shared mutational mechanism involving the expansion of a CAG repeat tract in coding regions of novel genes. Efforts have been made to identify biomarkers of disease progression, which would allow timely preventive therapeutic interventions. In the present study was assessed the influence of several genome instability biomarkers on SCA2 clinical severity...
April 2017: Archives of Medical Research
https://www.readbyqxmd.com/read/28922327/releasable-single-suture-for-primary-pterygium-excision-with-a-conjunctival-autograft
#5
Gautam Singh Parmar, Sachin Arya, Ashok Kumar Meena, Bhushan Ghodke, Elesh Jain, Budhendra Jain
PURPOSE: To evaluate the efficacy of a releasable single suture for pterygium excision with a conjunctival autograft (CAG). METHODS: Retrospective case series at a tertiary eye care center in central India was conducted. Medical records of 150 patients, who underwent primary pterygium excision with a CAG secured by a single releasable 10-0 nylon suture in the last year were reviewed. The surgical duration was noted. The suture was released on the first postoperative day under topical anesthesia...
September 15, 2017: Cornea
https://www.readbyqxmd.com/read/28920889/therapies-targeting-dna-and-rna-in-huntington-s-disease
#6
REVIEW
Edward J Wild, Sarah J Tabrizi
No disease-slowing treatment exists for Huntington's disease, but its monogenic inheritance makes it an appealing candidate for the development of therapies targeting processes close to its genetic cause. Huntington's disease is caused by CAG repeat expansions in the HTT gene, which encodes the huntingtin protein; development of therapies to target HTT transcription and the translation of its mRNA is therefore an area of intense investigation. Huntingtin-lowering strategies include antisense oligonucleotides and RNA interference targeting mRNA, and zinc finger transcriptional repressors and CRISPR-Cas9 methods aiming to reduce transcription by targeting DNA...
October 2017: Lancet Neurology
https://www.readbyqxmd.com/read/28920088/metabolic-and-transcriptomic-analysis-of-huntington-s-disease-model-reveal-changes-in-intracellular-glucose-levels-and-related-genes
#7
Gepoliano Chaves, Rıfat Emrah Özel, Namrata V Rao, Hana Hadiprodjo, Yvonne Da Costa, Zachary Tokuno, Nader Pourmand
Huntington's Disease (HD) is a neurodegenerative disorder caused by an expansion in a CAG-tri-nucleotide repeat that introduces a poly-glutamine stretch into the huntingtin protein (mHTT). Mutant huntingtin (mHTT) has been associated with several phenotypes including mood disorders and depression. Additionally, HD patients are known to be more susceptible to type II diabetes mellitus (T2DM), and HD mice model develops diabetes. However, the mechanism and pathways that link Huntington's disease and diabetes have not been well established...
August 2017: Heliyon
https://www.readbyqxmd.com/read/28918024/antisense-oligonucleotide-mediated-removal-of-the-polyglutamine-repeat-in-spinocerebellar-ataxia-type-3-mice
#8
Lodewijk J A Toonen, Frank Rigo, Haico van Attikum, Willeke M C van Roon-Mom
Spinocerebellar ataxia type 3 (SCA3) is a currently incurable neurodegenerative disorder caused by a CAG triplet expansion in exon 10 of the ATXN3 gene. The resultant expanded polyglutamine stretch in the mutant ataxin-3 protein causes a gain of toxic function, which eventually leads to neurodegeneration. One important function of ataxin-3 is its involvement in the proteasomal protein degradation pathway, and long-term downregulation of the protein may therefore not be desirable. In the current study, we made use of antisense oligonucleotides to mask predicted exonic splicing signals, resulting in exon 10 skipping from ATXN3 pre-mRNA...
September 15, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28917316/humoral-and-cytokine-response-elicited-during-immunisation-with-recombinant-immune-mapped-protein-1-etimp-1-and-oocysts-of-eimeria-tenella
#9
Krishnendu Kundu, Rajat Garg, Saroj Kumar, Mrityunjay Mandal, Fiona M Tomley, Damer P Blake, Partha Sarathi Banerjee
Eimeria tenella, the causative agent of caecal coccidiosis, is a pathogenic gut dwelling protozoan which can cause severe morbidity and mortality in farmed chickens. Immune mapped protein-1 (IMP-1) has been identified as an anticoccidial vaccine candidate; in the present study allelic polymorphism was assessed across the IMP-1 coding sequence in E. tenella isolates from four countries and compared with the UK reference Houghton strain. Nucleotide diversity was low, limited to expansion/contraction of a CAG triplet repeat and five substitutions, three of which were non-synonymous...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28915409/androgen-receptor-cytosine-adenine-and-guanine-trinucleotide-repeat-polymorphism-in-korean-patients-with-endometriosis-a-case-control-study
#10
Jae Jun Shin, Young Min Choi, Hwa Young Choi, Soo Jin Chae, Kyuri Hwang, Jin Ju Kim, Gyoung Hoon Lee, Jong Mi Kim
STUDY OBJECTIVE: To investigate the association between the androgen receptor (AR) cytosine, adenine, and guanine (CAG) repeat polymorphisms and endometriosis. STUDY DESIGN: A prospective case-control, genetic association study was performed on women with surgically proven endometriosis (n=421) and controls free of endometriosis (n=349). AR CAG repeat lengths were determined from peripheral blood samples. The difference in the frequency of each alleles were compared in patients with endometriosis and controls using Chi-square test...
September 8, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28904990/the-multiple-faces-of-spinocerebellar-ataxia-type-2
#11
REVIEW
Antonella Antenora, Carlo Rinaldi, Alessandro Roca, Chiara Pane, Maria Lieto, Francesco Saccà, Silvio Peluso, Giuseppe De Michele, Alessandro Filla
Spinocerebellar ataxia type 2 (SCA2) is among the most common forms of autosomal dominant ataxias, accounting for 15% of the total families. Occurrence is higher in specific populations such as the Cuban and Southern Italian. The disease is caused by a CAG expansion in ATXN2 gene, leading to abnormal accumulation of the mutant protein, ataxin-2, in intracellular inclusions. The clinical picture is mainly dominated by cerebellar ataxia, although a number of other neurological signs have been described, ranging from parkinsonism to motor neuron involvement, making the diagnosis frequently challenging for neurologists, particularly when information about the family history is not available...
September 2017: Annals of Clinical and Translational Neurology
https://www.readbyqxmd.com/read/28901944/vitamin-d-deficiency-in-pregnant-women-and-their-infants
#12
Abdurrahman Avar Özdemir, Yasemin Ercan Gündemir, Mustafa Küçük, Deniz Yıldıran Sarıca, Yusuf Elgörmüş, Yakup Çağ, Günal Bilek
OBJECTIVE: Vitamin D deficiency is a serious health problem although the improvement in socio-economic status in Turkey. The aim of this study was to evaluate the maternal vitamin D status and their effect on neonatal vitamin D status after support program for pregnant women and to detect risk factors for vitamin D deficiency in Bağcılar region in İstanbul. METHODS: A total of 97 pregnant women and 90 infants were included in this study between January and October 2016...
September 13, 2017: Journal of Clinical Research in Pediatric Endocrinology
https://www.readbyqxmd.com/read/28898235/associations-of-helicobacter-pylori-infection-and-chronic-atrophic-gastritis-with-accelerated-epigenetic-ageing-in-older-adults
#13
Xu Gao, Yan Zhang, Hermann Brenner
BACKGROUND: Helicobacter pylori (HP) infection and chronic atrophic gastritis (CAG) have shown strong associations with the development of gastric cancer. This study aimed to examine whether both risk factors are associated with accelerated epigenetic ageing, as determined by the 'DNA methylation age', in a population-based study of older adults (n=1477). METHODS: Serological measurements of HP antibodies and pepsinogen I and II for CAG definition were obtained by ELISA kits...
September 12, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28894060/-redo-off-pump-coronary-artery-bypass-for-postoperative-vein-graft-stenosis-of-anomalous-origin-of-the-left-coronary-artery-from-the-pulmonary-artery-report-of-a-case
#14
Taro Minamida, Takashi Sugiki, Masamichi Itou, Yasuhiro Kamikubo, Makoto Takahira
Bland-White-Garland (BWG) syndrome (anomalous origin of the left coronary artery from the pulmonary artery) is a rare disease which may result in myocardial infarction, congestive heart failure or sometimes death during the early infantile period. We present a 57-year-old female with BWG syndrome. At the age of 20, she was diagnosed with BWG syndrome and underwent coronary artery bypass grafting of a saphenous vein to the proximal portion of the anterior descending branch of the left coronary artery and ligation of the anomalous artery...
September 2017: Kyobu Geka. the Japanese Journal of Thoracic Surgery
https://www.readbyqxmd.com/read/28893843/timing-of-angiography-and-outcomes-in-high-risk-patients-with-non-st-segment-elevation-myocardial-infarction-managed-invasively-insights-from-the-tao-trial
#15
Pierre Deharo, Gregory Ducrocq, Christoph Bode, Marc Cohen, Thomas Cuisset, Shamir Mehta, Charles V Pollack, Stephen D Wiviott, Yedid Elbez, Marc Sabatine, P Gabriel Steg
Background -In patients with non ST-elevation myocardial infarction (NSTEMI) and Global Registry of Acute Coronary Events (GRACE) score >140, coronary angiography (CAG) is recommended by European and American guidelines within 24h. We sought to study the association of a "very early" (i.e. ≤12h), early (12-24h) and delayed (>24h) CAG in NSTEMI with GRACE score >140 with ischemic outcomes. Methods -The Treatment of Acute coronary syndrome with Otamixaban (TAO) trial randomized patients with NSTEMI and CAG scheduled within 72h to heparin plus eptifibatide versus otamixaban...
September 11, 2017: Circulation
https://www.readbyqxmd.com/read/28890334/crispr-mediated-base-editing-enables-efficient-disruption-of-eukaryotic-genes-through-induction-of-stop-codons
#16
Pierre Billon, Eric E Bryant, Sarah A Joseph, Tarun S Nambiar, Samuel B Hayward, Rodney Rothstein, Alberto Ciccia
Standard CRISPR-mediated gene disruption strategies rely on Cas9-induced DNA double-strand breaks (DSBs). Here, we show that CRISPR-dependent base editing efficiently inactivates genes by precisely converting four codons (CAA, CAG, CGA, and TGG) into STOP codons without DSB formation. To facilitate gene inactivation by induction of STOP codons (iSTOP), we provide access to a database of over 3.4 million single guide RNAs (sgRNAs) for iSTOP (sgSTOPs) targeting 97%-99% of genes in eight eukaryotic species, and we describe a restriction fragment length polymorphism (RFLP) assay that allows the rapid detection of iSTOP-mediated editing in cell populations and clones...
September 4, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28889771/helicobacter-pylori-infection-and-risk-of-preeclampsia-a-systematic-review-and-meta-analysis
#17
Malihe Nourollahpour Shiadeh, Seyed Mohammad Riahi, Ishag Adam, Vafa Saber, Zahra Behboodi Moghadam, Bahram Armon, Adel Spotin, Hajar Nazari Kangavari, Ali Rostami
OBJECTIVE: Helicobacter pylori is associated with many pregnancy adverse effects such as preeclampsia. We performed this systematic review and meta-analysis study to assess the possible association between H. pylori infection and preeclampsia (PE) and this is the first meta-analysis to clarify this issue. METHODS: PubMed, ISI (Web of science), SCOPUS and Google Scholar databases were searched (up to April 2017) to identify the relevant studies. The meta-analysis of observational studies in epidemiology (MOOSE) and PRISMA guidelines were used to do this study...
September 10, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28882590/targeted-deletion-of-rankl-in-m-cell-inducer-cells-by-the-col6a1-cre-driver
#18
Kazuki Nagashima, Shinichiro Sawa, Takeshi Nitta, Alejandro Prados, Vasiliki Koliaraki, George Kollias, Tomoki Nakashima, Hiroshi Takayanagi
The gut-associated lymphoid tissues (GALTs), including Peyer's patches (PPs), cryptopatches (CPs) and isolated lymphoid follicles (ILFs), establish a host-microbe symbiosis by the promotion of immune reactions against gut microbes. Microfold cell inducer (MCi) cells in GALTs are the recently identified mesenchymal cells that express the cytokine RANKL and initiate bacteria-specific immunoglobulin A (IgA) production via induction of microfold (M) cell differentiation. In the previous study, the Twist2-Cre driver was utilized for gene deletion in mesenchymal cells including MCi cells...
September 5, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28877561/a-severe-phenotype-of-kennedy-s-disease-associated-with-a-very-large-cag-repeat-expansion
#19
João Lo Madeira, Alexandre Bc Souza, Flavia S Cunha, Rafael L Batista, Nathalia L Gomes, Andresa S Rodrigues, Frederico Mennucci de Haidar Jorge, Gerson Chadi, Dagoberto Callegaro, Berenice B Mendonca, Elaine Mf Costa, Sorahia Domenice
No abstract text is available yet for this article.
September 6, 2017: Muscle & Nerve
https://www.readbyqxmd.com/read/28875587/a-new-mouse-line-for-cell-ablation-by-diphtheria-toxin-subunit-a-controlled-by-a-cre-dependent-flex-switch
#20
Nicholas W Plummer, Erica K Ungewitter, Kathleen G Smith, Humphrey H-C Yao, Patricia Jensen
Recombinase responsive mouse lines expressing diphtheria toxin subunit A (DTA) are well established tools for targeted ablation of genetically defined cell populations. Here we describe a new knock-in allele at the Gt(Rosa)26Sor locus that retains the best features of previously described DTA alleles-including a CAG promoter, attenuated mutant DTA cDNA, and ubiquitous EGFP labeling-with the addition of a Cre-dependent FLEx switch for tight control of expression. The FLEx switch consists of two pairs of antiparallel lox sites requiring Cre-mediated recombination for inversion of the DTA to the proper orientation for transcription...
September 5, 2017: Genesis: the Journal of Genetics and Development
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