Use the keywords feature with a free QxMD account.
Use Read by QxMD to access full text via your institution or open access sources.
Read also provides personalized recommendations to keep you up to date in your field.
Existing User
Sign InNew to Read
Sign Up#21
JOURNAL ARTICLE
Yi-Shan Cheng, Ye-Dong Sun, Jia-Ying Xing, Lu Zhan, Xiu-Jie Li, Jing Huang, Ming-Hui Zhao, Zhi-Fu Guo
Brassinosteroids (BR) play crucial roles in plant development and abiotic stresses in plants. Exogenous application of BR can significantly enhance cold tolerance in rice. However, the regulatory relationship between cold tolerance and the BR signaling pathway in rice remains largely unknown. Here, we characterized functions of the BR receptor OsBRI1 in response to cold tolerance in rice using its loss-of-function mutant (d61-1). Our results showed that mutant d61-1 was less tolerant to cold stress than wild-type (WT)...
February 29, 2024: Plant Physiology and Biochemistry: PPB
#22
JOURNAL ARTICLE
Ali Abdi Maalim, Zihan Wang, Yimin Huang, Ting Lei
Higher-grade meningiomas (WHO grade II and III) are characterized by aggressive invasiveness and high postoperative recurrence rates. The prognosis remains inadequate even with adjuvant radiotherapy and currently there is no definitive pharmacological treatment strategy and target for malignant meningiomas. This study aims to unveil the mechanisms driving the malignant progression of meningiomas and to identify potential inhibitory targets, with significant clinical implications. Implementing techniques such as protein immunoprecipitation, mass spectrometry, RNA interference, and transcriptome sequencing, we investigated the malignancy mechanisms in meningioma cell lines IOMM-LEE and CH157-MN...
February 13, 2024: Cancers
#23
JOURNAL ARTICLE
Tomonori Kaneko, Sally Ezra, Rober Abdo, Courtney Voss, Shanshan Zhong, Xuguang Liu, Owen Hovey, Marat Slessarev, Logan Robert Van Nynatten, Mingliang Ye, Douglas Fraser, Shawn Shun-Cheng Li
SARS-CoV-2 infection triggers extensive host immune reactions, leading to severe diseases in certain individuals. However, the molecular basis underlying the excessive yet non-productive immune responses in severe COVID-19 remains incompletely understood. In this study, we conducted a comprehensive analysis of the peripheral blood mononuclear cell (PBMC) proteome and phosphoproteome in sepsis patients positive or negative for SARS-CoV-2 infection, as well as healthy subjects, using quantitative mass spectrometry...
February 22, 2024: Clinical Proteomics
#24
JOURNAL ARTICLE
Xuemei Li, Qinghuan Yang, Peiran Jiang, Jun Wen, Yue Chen, Jiagui Huang, Mingfen Tian, Jiangxia Ren, Qin Yang
Fibrotic scars play important roles in tissue reconstruction and functional recovery in the late stage of nervous system injury. However, the mechanisms underlying fibrotic scar formation and regulation remain unclear. Casein kinase II (CK2) is a protein kinase that regulates a variety of cellular functions through the phosphorylation of proteins, including bromodomain-containing protein 4 (BRD4). CK2 and BRD4 participate in fibrosis formation in a variety of tissues. However, whether CK2 affects fibrotic scar formation remains unclear, as do the mechanisms of signal regulation after cerebral ischemic injury...
February 21, 2024: Neurochemical Research
#25
JOURNAL ARTICLE
Maria Ignatyeva, Abdul Kareem Mohideen Patel, Abdulkhaleg Ibrahim, Raed S Albiheyri, Ali T Zari, Ahmed Bahieldin, Christian Bronner, Jamal S M Sabir, Ali Hamiche
HIRIP3 is a mammalian protein homologous to the yeast H2A.Z deposition chaperone Chz1. However, the structural basis underlying Chz's binding preference for H2A.Z over H2A, as well as the mechanism through which Chz1 modulates histone deposition or replacement, remains enigmatic. In this study, we aimed to characterize the function of HIRIP3 and to identify its interacting partners in HeLa cells. Our findings reveal that HIRIP3 is specifically associated in vivo with H2A-H2B dimers and CK2 kinase. While bacterially expressed HIRIP3 exhibited a similar binding affinity towards H2A and H2A...
February 1, 2024: Cells
#26
Deepti Mudaliar, Rachel H Mansky, Angel White, Grace Baudhuin, Jon Hawkinson, Henry Wong, Michael A Walters, Rocio Gomez-Pastor
Protein Kinase CK2 is a holoenzyme composed of two regulatory subunits (CK2β) and two catalytic subunits (CK2α and CK2α'). CK2 controls several cellular processes including proliferation, inflammation, and cell death. However, CK2α and CK2α' possess different expression patterns and substrates and therefore impact each of these processes differently. Elevated CK2α participates in the development of cancer, while increased CK2α' has been associated with neurodegeneration, especially Huntington's disease (HD)...
January 22, 2024: bioRxiv
#27
JOURNAL ARTICLE
Qingzhu Liu, Shuyue Cai, Jiacong Ye, Quan Xie, Rongbin Liu, Ling Qiu, Jianguo Lin
PURPOSE: Neuropilin-1 (NRP-1) is a multifunctional protein involved in a variety of biological processes such as angiogenesis, tumorigenesis and immunomodulation. It was usually overexpressed in many cancer cell lines and correlated with poor prognosis of breast cancer. Positron emission tomography (PET) is an advanced imaging technique for detecting the function and metabolism of tumor-associated molecules in real time, dynamically, quantitatively and noninvasively. To improve the level of early diagnosis and evaluate the prognosis of breast cancer, an NRP-1 targeting peptide-based tracer [68 Ga]Ga-NOTA-PEG4 -CK2 was designed to sensitively and specifically detect the NRP-1 expression in vivo via PET imaging...
February 6, 2024: European Journal of Nuclear Medicine and Molecular Imaging
#28
JOURNAL ARTICLE
Alexandre Bancet, Rita Frem, Florian Jeanneret, Angélique Mularoni, Pauline Bazelle, Caroline Roelants, Jean-Guy Delcros, Jean-François Guichou, Catherine Pillet, Isabelle Coste, Toufic Renno, Christophe Battail, Claude Cochet, Thierry Lomberget, Odile Filhol, Isabelle Krimm
Although the involvement of protein kinase CK2 in cancer is well-documented, there is a need for selective CK2 inhibitors suitable for investigating CK2 specific roles in cancer-related biological pathways and further exploring its therapeutic potential. Here, we report the discovery of AB668, an outstanding selective inhibitor that binds CK2 through a bivalent mode, interacting both at the ATP site and an allosteric αD pocket unique to CK2. Using caspase activation assay, live-cell imaging, and transcriptomic analysis, we have compared the effects of this bivalent inhibitor to representative ATP-competitive inhibitors, CX-4945, and SGC-CK2-1...
February 16, 2024: IScience
#29
Shichang Liu, Erin Atkinson, Adriana Paulucci-Holthauzen, Bin Wang
No abstract text is available yet for this article.
February 5, 2024: Nature Communications
#30
JOURNAL ARTICLE
Nusrat Shafiq, Bushra Shakoor, Nazia Yaqoob, Shagufta Parveen, Simone Brogi, Ahmad Mohammad Salamatullah, Maryam Rashid, Mohammed Bourhia
Breast cancer is a major issue of investigation in drug discovery due to its rising frequency and global dominance. Plants are significant natural sources for the development of novel medications and therapies. Medicinal mushrooms have many biological response modifiers and are used for the treatment of many physical illnesses. In this research, a database of 89 macro-molecules with anti-breast cancer activity, which were previously isolated from the mushrooms in literature, has been selected for the three-dimensional quantitative structure-activity relationships (3D-QSAR) studies...
February 1, 2024: Journal of Biomolecular Structure & Dynamics
#31
JOURNAL ARTICLE
Ruiqi Sun, Yanyu Guo, Lilin Zhang, Huixia Zhang, Boxuan Yin, Xiaoyang Li, Changyan L, Liu Yang, Lei Zhang, Zexing Li, Jinhai Huang
Porcine reproductive and respiratory syndrome virus (PRRSV) is a major economically devastating pathogen that has evolved various strategies to evade innate immunity. Downregulation of antiviral interferon largely contributes to PRRSV immunoevasion through cytoplasmic melanoma differentiation-associated gene 5 (MDA5), a receptor that senses viral RNA. In this study, the downregulated transcription and expression levels of porcine MDA5 in PRRSV infection were observed, but the detailed mechanisms remain unclear...
January 23, 2024: Virologica Sinica
#32
JOURNAL ARTICLE
Maria Winiewska-Szajewska, Daniel Paprocki, Ewa Marzec, Jarosław Poznański
Histidine residues contribute to numerous molecular interactions, owing to their structure with the ionizable aromatic side chain with pKa close to the physiological pH. Herein, we studied how the two histidine residues, His115 and His160 of the catalytic subunit of human protein kinase CK2, affect the binding of the halogenated heterocyclic ligands at the ATP-binding site. Thermodynamic studies on the interaction between five variants of hCK2α (WT protein and four histidine mutants) and three ionizable bromo-benzotriazoles and their conditionally non-ionizable benzimidazole counterparts were performed with nanoDSF, MST, and ITC...
January 17, 2024: Scientific Reports
#33
JOURNAL ARTICLE
Ranjan Devkota, Jonnell C Small, Kaycee Carbone, Michael A Glass, Amedeo Vetere, Bridget K Wagner
Glucolipotoxicity (GLT), in which elevated levels of glucose and fatty acids have deleterious effects on β-cell biology, is thought to be one of the major contributors in progression of type 2 diabetes. In search of novel small molecules that protects β-cells against GLT, we previously discovered KD025, an inhibitor of Rho-associated coiled-coil containing kinase isoform 2 (ROCK2), as a GLT-protective compound in INS-1E cells and dissociated human islets. To further understand the mechanism of action of KD025, we found that pharmacological and genetic inhibition of ROCK2 was not responsible for the protective effects of KD025 against GLT...
January 11, 2024: Diabetes
#34
JOURNAL ARTICLE
Jilan A Nazeam, Sylvia A Boshra, Esraa Z Mohammed, Heba A El Gizawy
Ephedra is one of the oldest known medicinal plants and the largest genera of the Ephedraceae family. In vivo antitumor evaluation of Ephedra foeminea revealed that ethyl acetate (EtOAc) was the most bioactive fraction. Bio-guided fractionation of EtOAc fraction afforded nine compounds isolated for the first time from the plant species. Macrocyclic spermine alkaloids (1,9), proanthocyanidins (2,4,5), quinoline alkaloids (7,8), phenolic (3), and nucleoside (6) were identified and elucidated by spectroscopic analyses including 1D and 2D NMR, ESI-MS-MS spectrometry...
December 29, 2023: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
#35
JOURNAL ARTICLE
Yeseul Choi, Seong-Ryong Yu, Yujin Lee, Ann-Yae Na, Sangkyu Lee, Joseph Heitman, Ran Seo, Han-Seung Lee, Jong-Seung Lee, Yong-Sun Bahn
The casein kinase 2 (CK2) complex, crucial for eukaryotic growth, differentiation, and metabolic regulation, presents a promising therapeutic target for various human diseases, including cancer, diabetes, and obesity. Its potential as an antifungal target is further highlighted in this study, which explores CK2's functions in C. neoformans , a key fungal meningitis pathogen. The CK2 complex in C. neoformans , comprising the Cka1 catalytic subunit and Ckb1/2 regulatory subunits, is integral to processes like growth, cell cycle, morphogenesis, stress response, drug resistance, and virulence...
January 9, 2024: MBio
#36
JOURNAL ARTICLE
Ann Y Park, Michael Leney-Greene, Matthew Lynberg, Justin Q Gabrielski, Xijin Xu, Benjamin Schwarz, Lixin Zheng, Arasu Balasubramaniyam, Hyoungjun Ham, Brittany Chao, Yu Zhang, Helen F Matthews, Jing Cui, Yikun Yao, Satoshi Kubo, Jean Michel Chanchu, Aaron R Morawski, Sarah A Cook, Ping Jiang, Juan C Ravell, Yan H Cheng, Alex George, Aiman Faruqi, Alison M Pagalilauan, Jenna R E Bergerson, Sundar Ganesan, Samuel D Chauvin, Jahnavi Aluri, Joy Edwards-Hicks, Eric Bohrnsen, Caroline Tippett, Habib Omar, Leilei Xu, Geoffrey W Butcher, John Pascall, Elif Karakoc-Aydiner, Ayca Kiykim, Holden Maecker, İlhan Tezcan, Saliha Esenboga, Raul Jimenez Heredia, Deniz Akata, Saban Tekin, Altan Kara, Zarife Kuloglu, Emel Unal, Tanıl Kendirli, Figen Dogu, Esra Karabiber, T Prescott Atkinson, Claude Cochet, Odile Filhol, Catherine M Bosio, Mark M Davis, Richard P Lifton, Erika L Pearce, Oliver Daumke, Caner Aytekin, Gülseren Evirgen Şahin, Aysel Ünlüsoy Aksu, Gulbu Uzel, V Koneti Rao, Sinan Sari, Buket Dalgıç, Kaan Boztug, Deniz Cagdas, Sule Haskologlu, Aydan Ikinciogullari, David Schwefel, Silvia Vilarinho, Safa Baris, Ahmet Ozen, Helen C Su, Michael J Lenardo
Preserving cells in a functional, non-senescent state is a major goal for extending human healthspans. Model organisms reveal that longevity and senescence are genetically controlled, but how genes control longevity in different mammalian tissues is unknown. Here, we report a new human genetic disease that causes cell senescence, liver and immune dysfunction, and early mortality that results from deficiency of GIMAP5, an evolutionarily conserved GTPase selectively expressed in lymphocytes and endothelial cells...
February 2024: Nature Immunology
#37
JOURNAL ARTICLE
Joyce Eliza de Oliveira Souza, Shayane Martins Rodrigues Gomes, Ana Karina Castro Lima, Andréia Carolinne de Souza Brito, Silvia Amaral Gonçalves Da-Silva, Angela Hampshire de Carvalho Santos Lopes, Mário Alberto Cardoso Silva-Neto, Geórgia Correa Atella, Patricia Maria Lourenço Dutra
Chagas disease, endemic from Latin America, is caused by Trypanosoma cruzi and is transmitted by triatomine feces. This parasite undergoes complex morphological changes through its life cycle, promoted by significant changes in signal transduction pathways. The activity of protein kinase CK2 has been described in trypanosomatids. Using a specific peptide and radioactive ATP, we identified CK2 activity on the cellular surface and the cytoplasmic content in Trypanosoma cruzi, apart from the secreted form. Dephosphorylated casein promoted an increase of 48% in the secreted CK2 activity...
January 2, 2024: Parasitology Research
#38
REVIEW
Huilin Deng, Xinrui Rao, Sijia Zhang, Leichong Chen, Yan Zong, Rui Zhou, Rui Meng, Xiaorong Dong, Gang Wu, Qianwen Li
The protein kinase casein kinase 2 (CK2) exerts its influence on the metabolism of three major cellular substances by phosphorylating essential protein molecules involved in various cellular metabolic pathways. These substances include hormones, especially insulin, rate-limiting enzymes, transcription factors of key genes, and cytokines. This regulatory role of CK2 is closely tied to important cellular processes such as cell proliferation and apoptosis. Additionally, tumor cells undergo metabolic reprogramming characterized by aerobic glycolysis, accelerated lipid β-oxidation, and abnormally active glutamine metabolism...
December 29, 2023: BioFactors
#39
JOURNAL ARTICLE
Formulation and Investigation of CK2 Inhibitor-Loaded Alginate Microbeads with Different Excipients.
Boglárka Papp, Marc Le Borgne, Florent Perret, Christelle Marminon, Liza Józsa, Ágota Pető, Dóra Kósa, Lajos Nagy, Sándor Kéki, Zoltán Ujhelyi, Ádám Pallér, István Budai, Ildikó Bácskay, Pálma Fehér
The aim of this study was to formulate and characterize CK2 inhibitor-loaded alginate microbeads via the polymerization method. Different excipients were used in the formulation to improve the penetration of an active agent and to stabilize our preparations. Transcutol® HP was added to the drug-sodium alginate mixture and polyvinylpyrrolidone (PVP) was added to the hardening solution, alone and in combination. To characterize the formulations, mean particle size, scanning electron microscopy analysis, encapsulation efficiency, swelling behavior, an enzymatic stability test and an in vitro dissolution study were performed...
November 29, 2023: Pharmaceutics
#40
REVIEW
Dimitar Tonev, Albena Momchilova
The pathogenesis of multiple sclerosis (MS) suggests that, in genetically susceptible subjects, T lymphocytes undergo activation in the peripheral compartment, pass through the BBB, and cause damage in the CNS. They produce pro-inflammatory cytokines; induce cytotoxic activities in microglia and astrocytes with the accumulation of reactive oxygen species, reactive nitrogen species, and other highly reactive radicals; activate B cells and macrophages and stimulate the complement system. Inflammation and neurodegeneration are involved from the very beginning of the disease...
December 7, 2023: International Journal of Molecular Sciences
Make the most of Read.
Download the mobile app.
Download the mobile app.
Fetching more papers... 
