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MMP and prolapse

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https://www.readbyqxmd.com/read/27922684/excess-mechanical-stress-and-hydrogen-peroxide-remodel-extracellular-matrix-of-cultured-human-uterosacral-ligament-fibroblasts-by-disturbing-the-balance-of-mmps-timps-via-the-regulation-of-tgf%C3%A2-%C3%AE-1-signaling-pathway
#1
Qifan Zhang, Cheng Liu, Shasha Hong, Jie Min, Qing Yang, Ming Hu, Yang Zhao, Li Hong
The regulation of the extracellular matrix (ECM) by mechanical stress is of interest as the ECM is essential in the development of pelvic organ prolapse. In the present study, the effect of overexposure to mechanical stress on the ECM, and the probable underlying mechanisms in cultured human uterosacral ligament fibroblasts (hUSLFs), was explored. Mechanical stress has an effect on oxidation‑antioxidation products in parametrial ligament fibroblasts. Thus, hUSLFs were incubated with different concentrations of hydrogen peroxide to elucidate any potential interactions...
December 6, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27588175/matrix-metalloproteinase-3-gene-promoter-polymorphisms-a-potential-risk-factor-for-pelvic-organ-prolapse
#2
Charalampos Karachalios, Panagiotis Bakas, Georgios Kaparos, Styliani Demeridou, Ilias Liapis, Charalampos Grigoriadis, Aggelos Liapis
Pelvic organ prolapse (POP) is a common multifactorial condition. Matrix metalloproteinases (MMPs) are enzymes capable of breaking down various connective tissue elements. Single-nucleotide polymorphisms (SNPs) in regulatory areas of MMP-encoding genes can alter their transcription rate, and therefore the possible effect on pelvic floor supporting structures. The insertion of an adenine (A) base in the promoter of the MMP-3 gene at position -1612/-1617 produces a sequence of six adenines (6A), whereas the other allele has five (5A)...
September 2016: Biomedical Reports
https://www.readbyqxmd.com/read/27540720/matrix-metalloproteinase-1-expression-in-women-with-and-without-pelvic-organ-prolapse-a-systematic-review-and-meta-analysis
#3
Y Feng, Y Wang, B Yan, L Li, Y Deng
This meta-analysis was conducted to estimate the association between matrix metalloproteinase-1 (MMP-1) expression and pelvic organ prolapse (POP) in women. Relevant studies published before 6 December 2015 were identified by searching PubMed, Ovid, EBSCO, and EMBASE. A total number of five case-control studies, including 182 POP cases and 192 controls, were identified. The results indicated that women without POP had a lower MMP-1 level of expression compared with women with POP (odds ratio = 0.54, 95% confidence interval: 0...
August 19, 2016: Clinical and Translational Science
https://www.readbyqxmd.com/read/27124299/pelvic-organ-support-in-animals-with-partial-loss-of-fibulin-5-in-the-vaginal-wall
#4
Kathleen Chin, Cecilia Wieslander, Haolin Shi, Sunil Balgobin, T Ignacio Montoya, Hiromi Yanagisawa, R Ann Word
Compromise of elastic fiber integrity in connective tissues of the pelvic floor is most likely acquired through aging, childbirth-associated injury, and genetic susceptibility. Mouse models of pelvic organ prolapse demonstrate systemic deficiencies in proteins that affect elastogenesis. Prolapse, however, does not occur until several months after birth and is thereby acquired with age or after parturition. To determine the impact of compromised levels of fibulin-5 (Fbln5) during adulthood on pelvic organ support after parturition and elastase-induced injury, tissue-specific conditional knockout (cKO) mice were generated in which doxycycline (dox) treatment results in deletion of Fbln5 in cells that utilize the smooth muscle α actin promoter-driven reverse tetracycline transactivator and tetracycline responsive element-Cre recombinase (i...
2016: PloS One
https://www.readbyqxmd.com/read/27094962/host-response-to-synthetic-mesh-in-women-with-mesh-complications
#5
Alexis L Nolfi, Bryan N Brown, Rui Liang, Stacy L Palcsey, Michael J Bonidie, Steven D Abramowitch, Pamela A Moalli
BACKGROUND: Despite good anatomic and functional outcomes, urogynecologic polypropylene meshes that are used to treat pelvic organ prolapse and stress urinary incontinence are associated with significant complications, most commonly mesh exposure and pain. Few studies have been performed that specifically focus on the host response to urogynecologic meshes. The macrophage has long been known to be the key cell type that mediates the foreign body response. Conceptually, macrophages that respond to a foreign body can be dichotomized broadly into M1 proinflammatory and M2 proremodeling subtypes...
August 2016: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/26936098/collagen-metabolic-disorder-induced-by-oxidative-stress-in-human-uterosacral-ligament%C3%A2-derived-fibroblasts-a-possible-pathophysiological-mechanism-in-pelvic-organ-prolapse
#6
Cheng Liu, Qing Yang, Gui Fang, Bing-Shu Li, De-Bin Wu, Wen-Jun Guo, Sha-Sha Hong, Li Hong
Pelvic organ prolapse (POP) is a global health problem, for which the pathophysiological mechanism remains to be fully elucidated. The loss of extracellular matrix protein has been considered to be the most important molecular basis facilitating the development of POP. Oxidative stress (OS) is a well‑recognized mechanism involved in fiber metabolic disorders. The present study aimed to clarify whether OS exists in the uterosacral ligament (USL) with POP, and to investigate the precise role of OS in collagen metabolism in human USL fibroblasts (hUSLFs)...
April 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/25463637/systemic-markers-of-collagen-metabolism-and-vitamin-c-in-smokers-and-non-smokers-with-pelvic-organ-prolapse
#7
M Victoria Estanol, Catrina C Crisp, Susan H Oakley, Steven D Kleeman, Angela N Fellner, Rachel N Pauls
OBJECTIVE: To evaluate systemic markers of collagen metabolism and vitamin C in female smokers with pelvic organ prolapse (POP). Secondary aims were to compare these levels in women based on prolapse or smoking history alone. STUDY DESIGN: This was a cross-sectional study with four groups: smokers with POP, non-smokers with POP, smokers without POP and non-smokers without POP. Subjects were age-matched based on smoking history and presence of POP. All underwent a fasting blood panel, including plasma procollagen 1-N propeptide (P1NP), matrix metalloproteinase 9 (MMP-9), and vitamin C...
January 2015: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/25189765/functional-characteristics-of-vaginal-fibroblastic-cells-from-premenopausal-women-with-pelvic-organ-prolapse
#8
Alejandra M Ruiz-Zapata, Manon H Kerkhof, Behrouz Zandieh-Doulabi, Hans A M Brölmann, Theo H Smit, Marco N Helder
Pelvic organ prolapse (POP) remains a great therapeutic challenge with no optimal treatment available. Tissue maintenance and remodelling are performed by fibroblasts, therefore altered cellular functionality may influence tissue quality. In this study, we evaluated functional characteristics of fibroblastic cells from tissues involved in POP. To rule out normal ageing tissue degeneration, biopsies from 18 premenopausal women were collected from the precervical region (non-POP site) after hysterectomy of 8 healthy and 10 POP cystocele cases (POP-Q stage ≥ II)...
November 2014: Molecular Human Reproduction
https://www.readbyqxmd.com/read/25185601/transforming-growth-factor-%C3%AE-1-and-extracellular-matrix-protease-expression-in-the-uterosacral-ligaments-of-patients-with-and-without-pelvic-organ-prolapse
#9
Ava Leegant, Lisa C Zuckerwise, Keith Downing, Jurriaan Brouwer-Visser, Changcheng Zhu, Maria Jose Cossio, Felix Strube, Xianhong Xie, Erika Banks, Gloria S Huang
OBJECTIVES: This study was undertaken to evaluate the expression of transforming growth factor β1 (TGF-β1) and matrix metalloproteinase 9 (MMP-9), key regulators of the extracellular matrix composition, in the uterosacral ligaments (USLs) of women with pelvic organ prolapse (POP) compared with controls. METHODS: Under an institutional review board approval, USL samples were obtained from women undergoing vaginal hysterectomy for stage 2 or greater POP (cases, n = 21) and from women without POP undergoing vaginal hysterectomy for benign indications (controls, n = 19)...
January 2015: Female Pelvic Medicine & Reconstructive Surgery
https://www.readbyqxmd.com/read/25128444/impact-of-prolapse-meshes-on-the-metabolism-of-vaginal-extracellular-matrix-in-rhesus-macaque
#10
Rui Liang, Wenjun Zong, Stacy Palcsey, Steven Abramowitch, Pamela A Moalli
OBJECTIVE: The impact of polypropylene mesh implantation on vaginal collagen and elastin metabolism was analyzed using a nonhuman primate model to further delineate the mechanism of mesh induced complications. STUDY DESIGN: Forty-nine middle-aged parous rhesus macaques underwent surgical implantation of 3 synthetic meshes via sacrocolpopexy. Gynemesh PS (n = 12) (Ethicon, Somerville, NJ) and 2 lower-weight, higher-porosity, lower-stiffness meshes (UltraPro [n = 19] [Ethicon] and Restorelle [n = 8] [Coloplast, Minneapolis, MN]) were implanted, in which UltraPro was implanted with its blue orientation lines perpendicular (low stiffness direction, n = 11) and parallel (high stiffness direction, n = 8) to the longitudinal axis of the vagina...
February 2015: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/25110112/differential-expression-profiling-of-matrix-metalloproteinases-and-tissue-inhibitors-of-metalloproteinases-in-females-with-or-without-pelvic-organ-prolapse
#11
Xiaohong Wang, Yiqi Li, Jie Chen, Xiaoli Guo, Han Guan, Candong Li
Pelvic organ prolapse (POP) is a common disorder that can disturb the health and quality of life of females. However, the basic pathophysiology and underlying mechanism of POP are not fully understood. Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) have been reported to be associated with the onset and development of POP. In the present study, to characterize the differential expression profile of MMPs and TIMPs in female patients with and without POP, a total of 72 POP patients were sampled as a patient group and 72 non-POP patients that underwent hysterectomy due to benign tumors were sampled as a control group...
October 2014: Molecular Medicine Reports
https://www.readbyqxmd.com/read/24947034/effects-of-preoperative-local-estrogen-in-postmenopausal-women-with-prolapse-a-randomized-trial
#12
RANDOMIZED CONTROLLED TRIAL
David D Rahn, Meadow M Good, Shayzreen M Roshanravan, Haolin Shi, Joseph I Schaffer, Ravinder J Singh, R Ann Word
CONTEXT: Pelvic organ prolapse (POP) increases in prevalence with age; recurrence after surgical repair is common. OBJECTIVE: The objective of the study was to determine the effects of local estrogen treatment on connective tissue synthesis and breakdown in the vaginal wall of postmenopausal women planning surgical repair of POP. DESIGN: This was a randomized trial. SETTING: The study was conducted at an academic tertiary medical center...
October 2014: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/24670922/a-novel-role-of-sympathetic-activity-in-regulating-mitral-valve-prolapse
#13
Xiang Hu, Hao-Zhe Wang, Jun Liu, An-Qin Chen, Xiao-Feng Ye, Qiang Zhao
BACKGROUND: Increased sympathetic activity, commonly reported in mitral valve prolapse, indicates that the sympathetic nervous system might play an important role in regulating mitral interstitial cells. Hence, the aim of this study is to determine the level and pattern of adrenergic receptors expressed in human mitral valve leaflets and to investigate the effect of norepinephrine on physiologic behaviors of mitral interstitial cells. METHODS AND RESULTS: Immunohistochemistry displayed significantly increased expressions of β1, β2, and α1 adrenergic receptors in mitral valve prolapse...
2014: Circulation Journal: Official Journal of the Japanese Circulation Society
https://www.readbyqxmd.com/read/24343133/expression-of-extracellular-matrix-remodeling-proteins-is-altered-in-vaginal-tissue-of-premenopausal-women-with-severe-pelvic-organ-prolapse
#14
May Alarab, Hala Kufaishi, Stephen Lye, Harold Drutz, Oksana Shynlova
AIM: The molecular etiology of pelvic organ prolapse (POP) is complex and not well understood. We compared the expression/activity of extracellular matrix (ECM)-processing (procollagen I N-proteinase/ a disintegrin and metalloproteinase with thrombospondin motifs [ADAMTS]-2,-3,-14) and ECM-degrading (matrix metalloproteinase [MMP]-1, -2, -7, -8, -9, -12) enzymes and their natural tissue inhibitors (tissue inhibitors of metalloproteinase [TIMP]-1,-2,-3,-4) in vaginal tissues from premenopausal women with advanced POP (POP-Q stage ≥ 3) and asymptomatic controls (POP-Q = 0)...
June 2014: Reproductive Sciences
https://www.readbyqxmd.com/read/24202438/expression-of-matrix-metalloproteinase-1-in-round-ligament-and-uterosacral-ligament-tissue-from-women-with-pelvic-organ-prolapse
#15
Akın Usta, Kadir Guzin, Mehmet Kanter, Mustafa Ozgül, Ceyda Sancaklı Usta
To evaluate the matrix metalloproteinase-1 (MMP-1) expression in different parts of pelvic connective tissue in postmenopausal women with and without pelvic organ prolapse (POP). Ninety-one samples were obtained from only postmenopausal women (42 with POP and 49 non-POP subjects). All women were evaluated by pelvic organ prolapse quantitation. The POP group had stage 2 or more, and the controls had stage 1 or less. Round ligament (RL) and uterosacral ligament (USL) biopsies were obtained from women with POP and controls...
June 2014: Journal of Molecular Histology
https://www.readbyqxmd.com/read/24126727/is-anatomical-failure-following-anterior-vaginal-repair-associated-with-weak-native-vaginal-tissues-a-biomechanical-and-immunohistochemical-study
#16
A Khaja, P Winlove, M Waterfield, A Oriolowo, O A Adekanmi, R M Freeman
INTRODUCTION AND HYPOTHESIS: This study was performed to determine whether anatomical recurrence of cystocoele 1 year after anterior vaginal repair is related to biomechanical properties and/or the content of collagen in the vaginal wall and epithelial tissues. METHODS: In this prospective, observational study in a UK teaching hospital, we assessed women undergoing surgery for symptomatic anterior compartment prolapse. Outcome measures were anatomical recurrence, biomechanical strength and collagen content in vaginal tissues...
February 2014: International Urogynecology Journal
https://www.readbyqxmd.com/read/23727183/the-ethyl-acetate-fraction-of-polytrichum-commune-l-ex-hedw-induced-cell-apoptosis-via-reactive-oxygen-species-in-l1210-cells
#17
Xiaoxia Cheng, Yaping Xiao, Pan Wang, Xiaobing Wang, Yuetao Zhou, Han Yan, Quanhong Liu
ETHNOPHARMACOLOGICAL RELEVANCE: Polytrichum commune L.ex Hedw is a traditional Chinese herb for treatment of fever, hemostatic, uterine prolapse and especially for leukemia. Previous studies indicated its anti-leukemia effect but the potential mechanisms have not been fully explained. AIMS OF THE STUDY: The present study was further to investigate the underlying mechanism of ethyl acetate extract of Polytrichum commune L.ex Hedw (EEF)-induced toxicity and apoptosis in L1210 cells...
July 30, 2013: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/23437119/dysregulation-of-protease-and-protease-inhibitors-in-a-mouse-model-of-human-pelvic-organ-prolapse
#18
Madhusudhan Budatha, Simone Silva, Teodoro Ignacio Montoya, Ayako Suzuki, Sheena Shah-Simpson, Cecilia Karin Wieslander, Masashi Yanagisawa, Ruth Ann Word, Hiromi Yanagisawa
Mice deficient for the fibulin-5 gene (Fbln5(-/-)) develop pelvic organ prolapse (POP) due to compromised elastic fibers and upregulation of matrix metalloprotease (MMP)-9. Here, we used casein zymography, inhibitor profiling, affinity pull-down, and mass spectrometry to discover additional protease upregulated in the vaginal wall of Fbln5(-/-) mice, herein named V1 (25 kDa). V1 was a serine protease with trypsin-like activity similar to protease, serine (PRSS) 3, a major extrapancreatic trypsinogen, was optimum at pH 8...
2013: PloS One
https://www.readbyqxmd.com/read/23108733/the-polymorphisms-of-the-mmp-1-and-the-mmp-3-genes-and-the-risk-of-pelvic-organ-prolapse
#19
Paweł Skorupski, Katarzyna Jankiewicz, Paweł Miotła, Małgorzata Marczak, Beata Kulik-Rechberger, Tomasz Rechberger
INTRODUCTION AND HYPOTHESIS: To investigate the associations between single nucleotide polymorphism (SNP) type 1G/2G at position -1607/-1608 of the matrix metalloproteinase (MMP)-1 gene and SNP type 5A/6A at position -1612/-1617 of the MMP-3 gene and the development of pelvic organ prolapse (POP) in women. METHODS: 133 patients with symptomatic POP were included in the study group. The control group consisted of 132 women with a normal pelvic floor. 1G/2G MMP-1 and 5A/6A MMP-3 SNPs were determined by polymerase chain reaction (PCR) and restriction fragments length polymorphism analysis...
June 2013: International Urogynecology Journal
https://www.readbyqxmd.com/read/22944155/-role-of-canonical-wnt-signaling-transduction-pathway-in-the-pathogenesis-of-pelvic-organ-prolapse
#20
Zhen Wang, Hong-hui Shi, Guan Chen, Lan Zhu
OBJECTIVE: To explore the effects of canonical Wnt signaling transduction pathway on the proliferation and secretion of fibroblasts of uterosacral ligament so as to elucidate the pathogenic mechanism of pelvic organ prolapse (POP). METHODS: Five patients with grade ≥Ш POP and five with other benign gynecologic disorders as control were recruited. Specimens were taken from uterosacral ligaments and fibroblasts were cultured and purified. After the confirmation of cultured fibroblasts by immunohistochemical staining, their growth and proliferation activity were detected by thiazolyl blue tetrazolium bromide (MTT) assay and the expressions of matrix metal proteinase-2 (MMP-2), Wnt16, FZD5 and β-catenin by Western blot...
June 26, 2012: Zhonghua Yi Xue za Zhi [Chinese medical journal]
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