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https://www.readbyqxmd.com/read/29220670/qm-mm-modeling-of-the-hydrolysis-and-transfructosylation-reactions-of-fructosyltransferase-from-aspergillus-japonicas-an-enzyme-that-produces-prebiotic-fructooligosaccharide
#1
Jitrayut Jitonnom, James R Ketudat-Cairns, Supa Hannongbua
Fructosyltransferases (FTs) act on sucrose by cleaving the β-(2→1) linkage, releasing glucose, and then transferring the fructosyl group to an acceptor molecule. These enzymes are capable of producing prebiotic fructooligosaccharides (FOSs) that are of industrial interest. While several FOS-synthesizing enzymes FTs have been investigated, their catalytic mechanism is not yet fully understood, especially the molecular details of how FOS are enzymatically synthesized from sucrose. Here, we present a comparative quantum mechanics/molecular mechanics (QM/MM) study on the hydrolysis and transfructosylation reactions catalyzed by A...
November 24, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/29209072/free-sepf-interferes-with-recruitment-of-late-cell-division-proteins
#2
Yongqiang Gao, Michaela Wenzel, Martijs J Jonker, Leendert W Hamoen
The conserved cell division protein SepF aligns polymers of FtsZ, the key cell division protein in bacteria, during synthesis of the (Fts)Z-ring at midcell, the first stage in cytokinesis. In addition, SepF acts as a membrane anchor for the Z-ring. Recently, it was shown that SepF overexpression in Mycobacterium smegmatis blocks cell division. Why this is the case is not known. Surprisingly, we found in Bacillus subtilis that SepF overproduction does not interfere with Z-ring assembly, but instead blocks assembly of late division proteins responsible for septum synthesis...
December 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29202769/impact-of-systolic-blood-pressure-limits-on-the-diagnostic-value-of-triage-algorithms
#3
Tobias Neidel, Nicolas Salvador, Axel R Heller
BACKGROUND: Major incidents are characterized by a lack of resources compared to an overwhelming number of casualties, requiring a prioritization of medical treatment. Triage algorithms are an essential tool for prioritizing the urgency of treatment for patients, but the evidence to support one over another is very limited. We determined the influence of blood pressure limits on the diagnostic value of triage algorithms, considering if pulse should be palpated centrally or peripherally...
December 4, 2017: Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine
https://www.readbyqxmd.com/read/29201061/selective-and-irreversible-induction-of-necroptotic-cell-death-in-lung-tumorspheres-by-short-term-exposure-to-verapamil-in-combination-with-sorafenib
#4
Juan Sebastian Yakisich, Yogesh Kulkarni, Neelam Azad, Anand Krishnan V Iyer
The presence of highly resistant cancer cells and the toxicity to normal cells are key factors that limit chemotherapy. Here, we used two models of highly resistant lung cancer cells: (1) adherent cells growing under prolonged periods of serum starvation (PPSS) and (2) cells growing as floating tumorspheres (FTs) to evaluate the effect of Verapamil (VP) in combination with Sorafenib (SF). Compared to cells growing under routine culture conditions (RCCs), PPPS cells or FTs were highly sensitive to short-term exposure (24 h) to VP 100 μM + SF 5 μM (VP100 + SF5)...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29175905/x-ray-physico-chemical-imaging-during-activation-of-cobalt-based-fischer-tropsch-synthesis-catalysts
#5
Andrew M Beale, Simon D M Jacques, Marco Di Michiel, J Frederick W Mosselmans, Stephen W T Price, Pierre Senecal, Antonios Vamvakeros, James Paterson
The imaging of catalysts and other functional materials under reaction conditions has advanced significantly in recent years. The combination of the computed tomography (CT) approach with methods such as X-ray diffraction (XRD), X-ray fluorescence (XRF) and X-ray absorption near-edge spectroscopy (XANES) now enables local chemical and physical state information to be extracted from within the interiors of intact materials which are, by accident or design, inhomogeneous. In this work, we follow the phase evolution during the initial reduction step(s) to form Co metal, for Co-containing particles employed as Fischer-Tropsch synthesis (FTS) catalysts; firstly, working at small length scales (approx...
January 13, 2018: Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
https://www.readbyqxmd.com/read/29169362/influence-of-enhanced-recovery-after-surgery-programs-on-laparoscopy-assisted-gastrectomy-for-gastric-cancer-a-systematic-review-and-meta-analysis-of-randomized-control-trials
#6
REVIEW
Zhengyan Li, Qian Wang, Bofei Li, Bin Bai, Qingchuan Zhao
BACKGROUND: This meta-analysis is aimed to evaluate the feasibility and safety of enhanced recovery after surgery (ERAS) programs in gastric cancer patients undergoing laparoscopy-assisted gastrectomy (LAG). METHODS: We performed a meta-analysis of randomized control trials involving either enhanced recovery after surgery (ERAS)/fast track surgery (FTS) for patients underwent LAG. EMBASE, Pubmed, Web of science, and Cochrane Library were searched. Primary outcomes included the length of postoperative hospital stay, cost of hospitalization, postoperative complications, and readmission rate...
November 23, 2017: World Journal of Surgical Oncology
https://www.readbyqxmd.com/read/29150399/a-plate-waste-evaluation-of-the-farm-to-school-program
#7
Jaclyn D Kropp, Saul J Abarca-Orozco, Glenn D Israel, David C Diehl, Sebastian Galindo-Gonzalez, Lauren B Headrick, Karla P Shelnutt
OBJECTIVE: To investigate the impacts of the Farm to School (FTS) program on the selection and consumption of fruits and vegetables. DESIGN: Plate waste data were recorded using the visual inspection method before and after implementation of the program. SETTING: Six elementary schools in Florida: 3 treatment and 3 control schools. PARTICIPANTS: A total of 11,262 meal observations of National School Lunch Program (NSLP) participants in grades 1-5...
November 14, 2017: Journal of Nutrition Education and Behavior
https://www.readbyqxmd.com/read/29124415/spatial-and-temporal-distribution-of-carbon-dioxide-gas-using-gosat-data-over-iran
#8
Samereh Falahatkar, Seyed Mohsen Mousavi, Manochehr Farajzadeh
CO2 concentration (XCO2) shows the spatial and temporal variation in Iran. The major purpose of this investigation is the assessment of the spatial distribution of carbon dioxide concentration in the different seasons of 2013 based on the Thermal And Near Infrared Sensor for Carbon Observation-Fourier Transform Spectrometer (TANSO-FTS) level 2 GOSAT data by implementing the ordinary kriging (OK) method. In this study, the Land Surface Temperature (LST), Normalized Difference Vegetation Index (NDVI) data from the MODerate resolution Imaging Spectroradiometer (MODIS), and metrological parameters (temperature and precipitation) were used for the analysis of the spatial distribution of CO2 over Iran in 2013...
November 9, 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/29110100/gills-as-morphological-biomarkers-in-extensive-and-intensive-rainbow-trout-oncorhynchus-mykiss-walbaum-1792-production-technologies
#9
Emilia Strzyżewska-Worotyńska, Józef Szarek, Izabella Babińska, Dominika Gulda
We investigated environmental impacts on rainbow trout (Oncorhynchus mykiss) reared at fish farms with either extensive technology, in a flow-through system (FTS, n = 3), or intensive technology, in a recirculating aquaculture system (RAS, n = 3). All fish were fed the same rations. Fish were caught in spring and autumn (body mass, 501-750 g) from these six farms. We performed macroscopic (intact fish) and microscopic (gills stained with haematoxylin/eosin) examinations. Lesions were categorised based on the type and location of structural abnormalities...
November 6, 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/29077897/in-vivo-oxygen-temperature-and-ph-dynamics-in-the-female-reproductive-tract-and-their-importance-in-human-conception-a-systematic-review
#10
Ka Ying Bonnie Ng, Roel Mingels, Hywel Morgan, Nick Macklon, Ying Cheong
BACKGROUND: Despite advances in ART, implantation and pregnancy rates per embryo transfer still remain low. IVF laboratories strive to ensure that the process of handling gametes in vitro closely mimics the in vivo environment. However, there remains a lack of knowledge regarding the in vivo regulation and dynamic variation in biophysical parameters such as oxygen concentration, pH and temperature within the reproductive tract. OBJECTIVE AND RATIONALE: To undertake a systematic review of the current understanding of the physico-chemical parameters of oxygen tension (pO2), pH and temperature within the female reproductive tract, and their potential implications in clinical and pathological processes related to fertility and those pertaining to limited reproductive capacity...
October 25, 2017: Human Reproduction Update
https://www.readbyqxmd.com/read/29041194/highly-efficient-machining-of-non-circular-freeform-optics-using-fast-tool-servo-assisted-ultra-precision-turning
#11
Zexiao Li, Fengzhou Fang, Xiaodong Zhang, Xianlei Liu, Huimin Gao
Freeform optics has been regarded as the next generation of the optical components, especially those with non-circular apertures are playing an increasingly significant role in scanning field and specialized optical system. However, there still exist challenges to machine non-circular optical freeform surface. This paper is focused on highly efficiently generating freeform surfaces with optical surface quality by ultra-precision turning using a fast tool servo (FTS). A systematic strategy of machining smooth freeform surfaces with rectangular aperture is proposed in this paper...
October 16, 2017: Optics Express
https://www.readbyqxmd.com/read/29041118/broadband-fourier-transform-spectrometer-enabling-modal-subset-identification-in-fabry-p%C3%A3-rot-based-astrocombs
#12
Jake M Charsley, Richard A McCracken, Lauren Reid, Derryck T Reid
A multi-GHz frequency comb (astrocomb) is typically realized by filtering modes of a sub-GHz frequency comb (source comb) in a Fabry-Pérot etalon, which can lead to ambiguities in determining which subset of source comb modes has been filtered. Here we demonstrate a broadband Fourier-transform spectrometer (FTS) with a resolving power of R = 430,000 at 550 nm, and apply it to the identification of comb subsets from a filtered 1‑GHz supercontinuum. After apodization the FTS demonstrated an instrument line shape width of 1...
August 7, 2017: Optics Express
https://www.readbyqxmd.com/read/29032676/following-the-molecular-mechanism-of-decarbonylation-of-unsaturated-cyclic-ketones-using-bonding-evolution-theory-coupled-with-nci-analysis
#13
Ehsan Zahedi, Samaneh Shaabani, Abolfazl Shiroudi
The synergetic use of bonding evolution theory (BET) and noncovalent interaction (NCI) analysis allows to obtain new insight into the bond breaking/forming processes and electron redistribution along the reaction path to understand the molecular mechanism of a reaction and recognize regions of strong and weak electron pairing. This viewpoint has been considered for cheletropic extrusion of CO from unsaturated cyclic ketones cyclohepta-3,5-dien-1-one CHD, cyclopent-3-en-1-one CPE, and bicyclo[2.2.1]hept-2-en-7-one BCH by using hybrid functional MPWB1K in conjugation with aug-cc-pVTZ basis set...
November 1, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/29021972/evaluating-factors-for-prophylactic-feeding-tube-placement-in-gastroesophageal-cancer-patients-undergoing-chemoradiotherapy
#14
Vivek Verma, Pamela K Allen, Steven H Lin
PURPOSE: Though better studied in head/neck cancers, there are currently no studies on timing of feeding tube (FT) placement in patients with gastroesophageal cancer. This study sought to discern characteristics of patients who used versus did not use a prophylactic FT (pFT), and also analyzed factors associated with placement of FTs during chemoradiotherapy (CRT). METHODS/MATERIALS: From 1998 to 2013, 1,329 patients underwent neoadjuvant CRT, of which 323 received an FT...
2017: Frontiers in Oncology
https://www.readbyqxmd.com/read/28992213/repression-of-terminal-flower1-primarily-mediates-floral-induction-in-pear-pyrus-pyrifolia-nakai-concomitant-with-change-in-gene-expression-of-plant-hormone-related-genes-and-transcription-factors
#15
Songling Bai, Pham Anh Tuan, Takanori Saito, Akiko Ito, Benjamin Ewa Ubi, Yusuke Ban, Takaya Moriguchi
Floral induction is an important event in the annual growth cycle of perennial fruit trees. For pear, this event directly affects fruit production in the following year. The flower buds in many species are induced by FLOWERING LOCUS T (FT), whose effect is repressed by the meristem-expressed gene TERMINAL FLOWER1 (TFL1). In this study, we investigated the functions of pear FT and TFL1 genes during floral development. Expression of pear FTs (PpFT1a and PpFT2a) in reproductive meristems was not obviously induced prior to floral initiation, while expression of TFL1s (PpTFL1-1a and PpTFL1-2a) rapidly decreased...
September 7, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28959218/maternal-sensitivity-buffers-the-association-between-slc6a4-methylation-and-socio-emotional-stress-response-in-3-month-old-full-term-but-not-very-preterm-infants
#16
Livio Provenzi, Monica Fumagalli, Roberto Giorda, Francesco Morandi, Ida Sirgiovanni, Uberto Pozzoli, Fabio Mosca, Renato Borgatti, Rosario Montirosso
BACKGROUND: Very preterm (VPT) infants are hospitalized in Neonatal Intensive Care Units (NICUs) and are exposed to life-saving procedures eliciting pain-related stress. Recent research documented that pain-related stress might result in birth-to-discharge increased methylation of serotonin transporter gene (SLC6A4) in VPT infants, leading to poorer stress regulation at 3 months of age in VPT infants compared to their full-term (FT) counterparts. Maternal sensitivity is thought to support infants' stress response, but its role in moderating the effects of altered SLC6A4 methylation is unknown...
2017: Frontiers in Psychiatry
https://www.readbyqxmd.com/read/28949259/peripherally-inserted-central-catheters-dependency-of-radiation-exposure-from-puncture-site-and-level-of-training
#17
Martin Jonczyk, Bernhard Gebauer, Dirk Schnapauff, Roman Rotzinger, Bernd Hamm, Federico Collettini
Background Several interventional procedures show a dependency on fluoroscopy times (FT) and level of training. Furthermore, FT and dose area products (DAP) vary depending on access site and target vessel for chest port implantations, but not for other thoracic interventions such as percutaneous coronary interventions. Purpose To evaluate the influence of the combination of venous access site and level of training on FTs and DAPs during peripherally inserted central catheters (PICC) implantations in a large cohort of patients...
January 1, 2017: Acta Radiologica
https://www.readbyqxmd.com/read/28939742/integrated-analysis-reveals-tubal-and-ovarian-originated-serous-ovarian-cancer-and-predicts-differential-therapeutic-responses
#18
Dapeng Hao, Jingjing Li, Shanshan Jia, Yuan Meng, Li Wang, Chao Zhang, Li-Jun Di
PURPOSE: The relative importance of fallopian tube (FT) compared to ovarian surface epithelium (OSE) in the genesis of serous type of ovarian cancer (SOC) is still unsettled. Here, we followed an integrated approach to study the tissue origin of SOC, as well as its association with clinical outcome and response to therapeutic drugs. EXPERIMENTAL DESIGN: A collection of transcriptome data of 80 FTs, 89 OSEs and 2,668 SOCs was systematically analyzed to determine the characteristic of FT-like and OSE-like tumors...
September 22, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28932707/neisseria-gonorrhoeae-challenge-increases-matrix-metalloproteinase-8-expression-in-fallopian-tube-explants
#19
Natalia E Juica, Paula I Rodas, Paula Solar, Paula Borda, Renato Vargas, Cristobal Muñoz, Rodolfo Paredes, Myron Christodoulides, Luis A Velasquez
Background:Neisseria gonorrhoeae (Ngo) is the etiological agent of gonorrhea, a sexually transmitted infection that initially infects the female lower genital tract. In untreated women, the bacteria can ascend to the upper genital reproductive tract and infect the fallopian tube (FTs), which is associated with salpingitis and can lead to impaired FT function and infertility. The extracellular matrix (ECM) plays an important role in cell migration and differentiation in the female genital tract, and some pathogens modify the ECM to establish successful infections...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28892505/frailty-is-associated-with-objectively-assessed-sedentary-behaviour-patterns-in-older-adults-evidence-from-the-toledo-study-for-healthy-aging-tsha
#20
Borja Del Pozo-Cruz, Asier Mañas, María Martín-García, Jorge Marín-Puyalto, Francisco J García-García, Leocadio Rodriguez-Mañas, Amelia Guadalupe-Grau, Ignacio Ara
OBJECTIVE: The aim of this study was to examine the association of sedentary behaviour patterns with frailty in older people. SETTING: Clinical setting. DESIGN: Cross-sectional, observational study. PARTICIPANTS AND MEASUREMENTS: A triaxial accelerometer was used in a subsample from the Toledo Study for Healthy Aging (519 participants, 67-97 years) to assess several sedentary behaviour patterns including sedentary time per day, the number and duration (min) of breaks in sedentary time per day, and the proportion of the day spent in sedentary bouts of 10 minutes or more...
2017: PloS One
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