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https://www.readbyqxmd.com/read/27897453/clinical-usefulness-of-simultaneous-measurement-of-the-tear-levels-of-ccl17-ccl24-and-il-16-for-the-biomarkers-of-allergic-conjunctival-disorders
#1
Jun Shoji, Hiroshi Aso, Noriko Inada
PURPOSE: This study investigated the clinical usefulness of a multiple tear cytokine/chemokine test by simultaneously determining tear levels of CC chemokine ligand 17 (CCL17)/thymus and activation-regulated chemokine (TARC), CCL24/eotaxin-2, and interleukin-16 (IL-16) for assessing acute and chronic allergic inflammation in allergic conjunctival disorders (ACDs). SUBJECTS AND METHODS: This study included 37 patients with ACD and 11 healthy adults (controls). Patients with ACDs were divided into the following three groups; patients with allergic conjunctivitis (AC group, n = 17), patients with atopic keratoconjunctivitis (AKC group, n = 6), and patients with vernal keratoconjunctivitis (VKC group, n = 14)...
November 29, 2016: Current Eye Research
https://www.readbyqxmd.com/read/27891662/acute-mechanical-hemolysis-as-a-complication-of-extracorporeal-photopheresis-in-a-low-weight-child
#2
Robert A DeSimone, Sandeep N Wontakal, Alexander K Lyashchenko, Joseph Schwartz
Graft-versus-host disease (GVHD) is a complication of allogeneic hematopoietic stem cell transplantation with high morbidity and mortality. Extracorporeal photopheresis (ECP) is an effective therapy for treating medication-refractory GVHD, however, there is scant evidence of whether ECP can be safely performed in patients weighing less than 15 kg. Here, we report the implementation of a successful protocol to perform ECP in a 21-month-old, 10.6 kg female with medication-refractory GVHD. Our initial ECP treatment resulted in significant hemolysis that was most likely mechanical...
November 28, 2016: Journal of Clinical Apheresis
https://www.readbyqxmd.com/read/27889121/increasing-estrus-expression-in-the-lactating-dairy-cow
#3
J A Sauls, B E Voelz, S L Hill, L G D Mendonça, J S Stevenson
Using an activity monitoring system (AMS) equipped with an accelerometer, 2 experiments were conducted to test the hypotheses that (1) enhancing progesterone before inducing luteolysis or (2) exposing cows to estradiol cypionate (ECP) or testosterone propionate (TP) after luteolysis would increase occurrence and intensity of estrus. Our goal was to determine if more cows could be detected in estrus by an AMS compared with other estrus-detection aids. In experiment 1, cows (n = 154) were fitted with both an AMS collar and a pressure-sensitive, rump-mounted device (HeatWatch; HW) and assigned to 3 treatments: (1) no CL + progesterone insert (CIDR) for 5 d, (2) CL only, or (3) CL + 2 CIDR inserts for 5 d to achieve a range in concentrations of progesterone...
November 23, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27888642/histamine-h4-receptor-gene-polymorphisms-a-potential-predictor-of-oral-h1-antihistamine-efficacy-for-allergic-rhinitis
#4
Jian Gu, Xiao-Hui Mao, Xi-Zhi Yang, Hua-Fei Ao, Zhe Zhang, You Li
BACKGROUND: Our study aimed to investigate the associations between Histamine H4 receptor (HRH4) gene polymorphisms (rs77485247, rs74604924, and rs77041280) and oral H1 antihistamine efficacy for the treatment of allergic rhinitis (AR) patients. METHODS: A total of 142 AR patients were selected as a case group and 160 healthy individuals were recruited as a control group. Single nucleotide polymorphisms (SNPs) in the HRH4 gene were detected using direct sequencing...
November 26, 2016: International Forum of Allergy & Rhinology
https://www.readbyqxmd.com/read/27884809/construction-of-a-streptococcus-agalactiae-phob-mutant-and-evaluation-of-its-potential-as-an-attenuated-modified-live-vaccine-in-golden-pompano-trachinotus-ovatus
#5
Xiaohui Cai, Bei Wang, Yinhui Peng, Yuan Li, Yishan Lu, Yucong Huang, Jichang Jian, Zaohe Wu
Streptococcus agalactiae is a Gram-positive pathogen that can survive inside professional phagocytes and nonphagocytic cells to cause septicemia and meningoencephalitis in freshwater and marine fish. However, vaccines based on extracellular products (ECP) and formalin-killed whole S. agalactiae cells, as well as subunit vaccine are unable to protect fish from infection by variant serotypes S. agalactiae. The search for live attenuated vaccine with highly conserved and virulent-related genes is essential for producing a vaccine to help understand and control streptococcosis In this study, the phoB gene was cloned from pathogenic S...
November 21, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27883241/neutrophil-autophagy-and-extracellular-dna-traps-contribute-to-airway-inflammation-in-severe-asthma
#6
Duy Le Pham, Ga-Young Ban, Seung-Hyun Kim, Yoo Seob Shin, Young-Min Ye, Yong-Joon Chwae, Hae-Sim Park
BACKGROUND: Autophagy and neutrophil extracellular DNA traps (NETs) are implicated in asthma; however, their roles in asthma pathogenesis have not been elucidated. OBJECTIVES: We compared autophagy and NET production levels from peripheral blood neutrophils (PBNs) of patients with severe asthma (SA) and non-severe asthma (NSA). Additionally, we investigated the inflammatory effects of NETs on human airway epithelial cells (AECs) and peripheral blood eosinophils (PBEs)...
November 24, 2016: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/27882015/are-serum-eosinophilic-cationic-protein-levels-of-toll-collectors-affected-by-diesel-exhaust-exposure
#7
Cahit Bilgin, Peri Arbak, Ozlem Yavuz, Ege Gulec Balbay, Oner Balbay, Ali Nihat Annakkaya
OBJECTIVE: There are few studies on the diesel exhaust particulates (DEP) / eosinophilic cationic protein (ECP) level relationship. This study aimed to detect ECP levels in a highly DE exposed group, named as toll collectors. METHODS: In a cross-sectional study, levels of serum ECP, rates of respiratory symptoms, mean levels of respiratory functions, smoking status, and variations in peak expiratory flow (PEF) during weekends and working days were compared for 68 toll collectors (TC) (range of age, 24-48 years) and 28 controls (range of age, 25-61 years)...
September 2016: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/27869585/the-critical-components-of-an-electronic-care-plan-tool-for-primary-care-an-exploratory-qualitative-study
#8
Lisa Rotenstein, Suhavi Tucker, Rose Kakoza, Lori Tishler, Adrian Zai, Charlotte Wu
BACKGROUND: A critical need exists for effective electronic tools that facilitate multidisciplinary care for complex patients in patient-centered medical homes.Objective To identify the essential components of a primary care (PC) based electronic care plan (ECP) tool that facilitates coordination of care for complex patients. METHOD: Three focus groups and nine semi-structured interviews were conducted at an academic PC practice in order to identify the ideal components of an ECP...
July 4, 2016: Journal of Innovation in Health Informatics
https://www.readbyqxmd.com/read/27861297/extracorporeal-photopheresis-for-treatment-of-acute-and-chronic-graft-versus-host-disease-an-italian-multicentric-retrospective-analysis-on-94-patients-on-behalf-of-the-gruppo-italiano-trapianto-di-midollo-osseo
#9
Michele Malagola, Valeria Cancelli, Cristina Skert, Pierino Ferremi Leali, Emilio Ferrari, Alessandra Tiburzi, Maria Luisa Sala, Irene Donnini, Patrizia Chiusolo, Alberto Mussetti, Marta Battista, Alessandro Turra, Federica Cattina, Benedetta Rambaldi, Francesca Schieppati, Nicola Polverelli, Simona Bernardi, Simone Perucca, Mirella Marini, Daniele Laszlo, Chiara Savignano, Francesca Patriarca, Paolo Corradini, Nicola Piccirillo, Simona Sica, Alberto Bosi, Domenico Russo
BACKGROUND: Extracorporeal photopheresis (ECP) is considered a valid second-line treatment for acute and chronic graft versus host disease (GVHD). METHODS: Ninety-four patients with acute GVHD (aGVHD) (n = 45) and chronic GVHD (cGVHD) (n = 49), retrospectively recruited in 6 Italian centers, were submitted to ECP for second-line treatment. At the time of ECP, 22 (49%) and 23 (51%) of 45 patients with aGHVD were nonresponsive and in partial remission (PR) after steroids, respectively, and all the 49 patients with cGVHD were steroid refractory...
December 2016: Transplantation
https://www.readbyqxmd.com/read/27826294/evidence-for-cleavage-of-the-metalloprotease-vsm-from-vibrio-splendidus-strain-jz6-by-an-m20-peptidase-pept-like-protein-at-low-temperature
#10
Rui Liu, Limei Qiu, Qi Cheng, Huan Zhang, Lingling Wang, Linsheng Song
Metalloprotease Vsm is a major extracellular virulence factor of Vibrio splendidus. The toxicity of Vsm from V. splendidus strain JZ6 has been characterized, and production of this virulence factor proved to be temperature-regulated. The present study provides evidence that two forms (JZE1 and JZE2) of Vsm protein exist in extracellular products (ECPs) of strain JZ6, and a significant conversion of these two forms was detected by SDS-PAGE and immunoblotting analyses of samples obtained from cells grown at 4, 10, 16, 20, 24, and 28°C...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27807906/activin-a-and-follistatin-in-patients-with-asthma-does-severity-make-the-difference
#11
Anastasia Papaporfyriou, Petros Bakakos, Konstantinos Kostikas, Georgios Papatheodorou, Georgios Hillas, Rodoula Trigidou, Patroklos Katafigiotis, Nikolaos G Koulouris, Spyros A Papiris, Stelios Loukides
BACKGROUND AND OBJECTIVE: Activin A is a pleiotropic cytokine holding a fundamental role in inflammation and tissue remodelling. Follistatin can modulate the bioactivity of activin. We aimed to measure activin A and follistatin in sputum supernatants and bronchoalveolar lavage (BAL) of asthmatic patients and to determine the possible associations with severity as well as with inflammatory and remodelling indices. METHODS: A total of 58 asthmatic patients (33 with severe refractory asthma (SRA)) and 10 healthy controls underwent sputum induction for % cells, activin A, follistatin, eosinophilic cationic protein (ECP), transforming growth factor beta 1 (TGF-β1), IL-13 and IL-8 measurements...
November 2, 2016: Respirology: Official Journal of the Asian Pacific Society of Respirology
https://www.readbyqxmd.com/read/27804973/general-hyperconcentration-of-photonic-polarization-time-bin-hyperentanglement-assisted-by-nitrogen-vacancy-centers-coupled-to-resonators
#12
Fang-Fang Du, Fu-Guo Deng, Gui-Lu Long
Entanglement concentration protocol (ECP) is used to extract the maximally entangled states from less entangled pure states. Here we present a general hyperconcentration protocol for two-photon systems in partially hyperentangled Bell states that decay with the interrelation between the time-bin and the polarization degrees of freedom (DOFs), resorting to an input-output process with respect to diamond nitrogen-vacancy centers coupled to resonators. We show that the resource can be utilized sufficiently and the success probability is largely improved by iteration of the hyper-ECP process...
November 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27770460/alcohol-hyper-responsiveness-in-chronic-rhinosinusitis-with-nasal-polyps
#13
Els De Schryver, Lara Derycke, Paloma Campo, Eline Gabriels, Guy F Joos, Thibaut Van Zele, Claus Bachert, Peter W Hellings, Philippe Gevaert
BACKGROUND: An important percentage of subjects diagnosed with chronic upper airway disease report alcohol-induced worsening of their symptoms. The prevalence and characteristics of respiratory reactions provoked by alcohol-containing drinks has not been fully investigated yet. OBJECTIVE: The aim of this study was to estimate the prevalence and characteristics of alcohol hyper-responsiveness in patients with chronic airway disease and healthy controls. Furthermore, nasal inflammation was evaluated in nasal polyp patients with and without hyper-responsiveness...
October 22, 2016: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/27765572/mouse-eosinophil-associated-ribonucleases-mechanism-of-cytotoxic-antibacterial-and-antiparasitic-activities
#14
Ayush Attery, Janendra K Batra
Ribonuclease A family is a group of proteins having similar structures and catalytic mechanism but different functions. Human eosinophil granules contain two ribonucleases belonging to the RNase A family, eosinophil cationic protein (ECP) and eosinophil derived neurotoxin (EDN). In mouse, 15 orthologs of EDN and ECP, called mouse eosinophil associated ribonucleases (mEARs) have been reported which are expressed under different pathophysiological conditions. In this study, we have characterized mEAR2, mEAR5, mEAR7 and mEAR11, and compared them with ECP for their catalytic, cytotoxic, antibacterial and antiparasitic activities...
October 17, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27760844/injuries-in-an-extreme-conditioning-program
#15
Kyle T Aune, Joseph M Powers
BACKGROUND: Extreme conditioning programs (ECPs) are fitness training regimens relying on aerobic, plyometric, and resistance training exercises, often with high levels of intensity for a short duration of time. These programs have grown rapidly in popularity in recent years, but science describing the safety profile of these programs is lacking. HYPOTHESIS: The rate of injury in the extreme conditioning program is greater than the injury rate of weightlifting and the majority of injuries occur to the shoulder and back...
October 19, 2016: Sports Health
https://www.readbyqxmd.com/read/27742969/stress-coping-styles-of-459%C3%A2-emergency-care-physicians-in%C3%A2-germany-a%C3%A2-pilot-study
#16
M Sand, S Hessam, D Sand, F G Bechara, C Vorstius, M Bromba, E Stockfleth, I Shiue
AIM: In addition to assessing stress-coping strategies in patients, equal attention should be paid to health-care professionals. The literature on the stress-coping strategies of emergency physicians - health-care professionals who are frequently subject to stress in a fast-paced clinical setting - is scant. Therefore, we aimed to investigate the stress-coping strategies of emergency-care physicians (ECPs) in Germany. METHODS: We conducted a cross-sectional study by approaching German Associations of Emergency Medicine Physicians and the two largest ECP recruitment agencies in Germany to invite their members to participate...
October 14, 2016: Der Anaesthesist
https://www.readbyqxmd.com/read/27741338/75-complete-response-and-15-partial-response-to-extracorporeal-photopheresis-combined-with-other-therapies-in-resistant-early-stage-cutaneous-t-cell-lymphoma
#17
Sila Seremet, Sunil Abhyankar, Tiffany J Herd, Daniel Aires
INTRODUCTION: Extracorporeal photopheresis (ECP) has been used for the treatment of advanced stage or treatment refractory cutaneous T-cell lymphoma (CTCL) since 1987, and more recently has also been shown to be of benefit for earlier stage resistant CTCL. Complete response rates in prior studies of ECP in early CTCL have ranged from 0% to 40%. METHODS: We reviewed electronic medical records of all CTCL patients seen in the University of Kansas Cancer Center between June 2007 and May 2011...
October 1, 2016: Journal of Drugs in Dermatology: JDD
https://www.readbyqxmd.com/read/27739258/physicians-knowledge-of-and-attitudes-toward-use-of-opioids-in-long-term-care-facilities
#18
Charlotte Griffioen, Eva G Willems, Sanne M Kouwenhoven, Monique A A Caljouw, Wilco P Achterberg
BACKGROUND: Insufficient pain management in vulnerable older persons living in long-term care facilities is common, and opiophobia might contribute to this. As opiophobia and its related factors have not been investigated in long-term care, this study evaluates the degree of knowledge of opioids among elderly-care physicians (ECPs) and ECP trainees, as well as their attitudes and other factors possibly influencing the clinical use of opioids in these facilities. METHODS: A questionnaire was designed and distributed among ECPs and ECP trainees by email, regional symposia, and all three university training faculties for elderly-care medicine in the Netherlands...
October 14, 2016: Pain Practice: the Official Journal of World Institute of Pain
https://www.readbyqxmd.com/read/27739024/expression-of-inflammatory-mediators-in-induced-sputum-comparative-study-in-asthma-and-copd
#19
Magdalena Paplińska-Goryca, Patrycja Nejman-Gryz, Katarzyna Górska, Katarzyna Białek-Gosk, Joanna Hermanowicz-Salamon, Rafał Krenke
Asthma and COPD are the most common obstructive lung diseases characterized by inflammation in the lower airways which contribute to airflow limitation. Different inflammatory mediators are thought to play a key role in these diseases. This study was conducted in 13 patients with asthma, 12 patients with COPD, and 13 control subjects. The expression of mRNA of IL-6, IL-13, CXCL8, TSLP, IL-33, IL-25, IL-17, ECP, mast cell tryptase, CCL24, and CCL26 was assessed in induced sputum cells by real time PCR. We found that CXCL8 was strongly related to the neutrophil percentage but differed significantly in COPD and asthma patients...
October 14, 2016: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/27722102/comparison-of-protein-expression-pattern-between-the-plasmodium-falciparum-chloroquine-resistant-rkl9-and-chloroquine-sensitive-mrc2-strains
#20
Hiasindh Ashmi Antony, Vrushali Pathak, Kanjaksha Ghosh, Subhash Chandra Parija
OBJECTIVE: The objective of this study was to compare the protein expression patterns of Plasmodium falciparum extracellular and intracellular proteins separated by two-dimensional electrophoresis (2-DE) from the chloroquine-sensitive (CQS) MRC2 strain and chloroquine-resistant (CQR) RKL9 strain. Materials and Methods: Both the extracellular protein (ECP) and intracellular protein (ICP) were extracted and solubilized. The proteins were separated by 2-DE, first based on their charges using isoelectric focusing and then their sizes by electrophoresis...
July 2016: Tropical Parasitology
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