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https://www.readbyqxmd.com/read/28748172/protective-effect-of-tribulus-terrestris-fruit-extract-on-cerulein-induced-acute-pancreatitis-in-mice
#1
Mina Borran, Mohsen Minaiyan, Behzad Zolfaghari, Parvin Mahzouni
OBJECTIVE: Antioxidant, anti-inflammatory, analgesic and antimicrobial activities of Tribulus terrestris (T. terrestris) could be helpful in the treatment of acute pancreatitis; thus, this study was designed to investigate the effects of T. terrestris on cerulein-induced acute pancreatitis in mice. MATERIALS AND METHODS: Three doses (100, 200 and 400 mg/kg) of T. terrestris hydro-alcoholic extract were administered both orally (60 minutes before pancreatitis induction, p...
May 2017: Avicenna Journal of Phytomedicine
https://www.readbyqxmd.com/read/28747869/effect-of-dietary-copper-addition-on-lipid-metabolism-in-rabbits
#2
Liu Lei, Sui Xiaoyi, Li Fuchang
The present study was conducted to investigate the effect of copper supplementation on lipid metabolism in rabbits. Our study showed dietary copper addition (5-45 mg/kg) increased body mass gain, but decreased fat and liver weights compared with those in the control group (P < 0.05). Copper (45 mg/kg) addition significantly increased the skeletal muscle weight, but inhibited cytoplasmic lipid accumulation in liver, skeletal muscle and adipose tissue (P < 0.05). Compared with the control group, dietary copper addition (45 mg/kg) significantly increased plasma triglyceride levels but decreased very low density lipoprotein levels (P < 0...
2017: Food & Nutrition Research
https://www.readbyqxmd.com/read/28747860/lipolytic-efficacy-of-alginate-double-layer-nanoemulsion-containing-oleoresin-capsicum-in-differentiated-3t3-l1-adipocytes
#3
Mak-Soon Lee, Sunyoon Jung, Yoonjin Shin, Seohyun Lee, Chong-Tai Kim, In-Hwan Kim, Yangha Kim
Background: Oleoresin capsicum (OC) is an organic extract from fruits of the genus Capsicum, and has been reported to have an anti-obesity effect. Objective: This study comparatively investigated lipolytic effects of single-layer nanoemulsion (SN) and alginate double-layer nanoemulsion (AN) containing OC in 3T3-L1 adipocytes. Methods: SN and AN were compared by analyzing the intracellular lipid accumulation, triglyceride (TG) content, release of free fatty acids (FFAs) and glycerol, and mRNA expression of genes related to adipogenesis and lipolysis were analyzed in fully differentiated 3T3-L1 adipocytes...
2017: Food & Nutrition Research
https://www.readbyqxmd.com/read/28747058/pancreatic-enzyme-replacement-therapy-in-cystic-fibrosis-dose-variability-and-coefficient-of-fat-absorption
#4
Joaquim Calvo-Lerma, Sandra Martínez-Barona, Etna Masip, Victoria Fornés, Carmen Ribes-Koninckx
OBJECTIVES: Pancreatic enzyme replacement therapy (PERT) remains a backbone in the nutritional treatment of cystic fibrosis. Currently, there is a lack of an evidence-based tool that allows dose adjustment. To date, no studies have found an association between PERT dose and fat absorption. Therefore, the aim of the study was to assess the influence of both the PERT dose and the variability in this dose on the coefficient of fat absorption (CFA). METHODS: This is a retrospective longitudinal study of 16 pediatric patients (192 food records) with three consecutive visits to the hospital over a twelve-month period...
July 27, 2017: Revista Española de Enfermedades Digestivas
https://www.readbyqxmd.com/read/28745876/ionic-liquids-as-tool-to-improve-enzymatic-organic-synthesis
#5
Toshiyuki Itoh
Ionic liquids (ILs) have now been acknowledged as reaction media for biotransformations. The first three examples were reported in this field in 2000, and since then, numerous applications have been reported for biocatalytic reactions using ILs. Two topics using ILs for enzymatic reactions have been reviewed from the standpoint of biocatalyst mediating organic synthesis; the first is "Biocatalysis in Ionic Liquids" which includes various types of biocatalytic reactions in ILs (section 2): (1) recent examples of lipase-mediated reactions using ILs as reaction media for biodiesel oil production and for sugar ester production, (2) oxidase-catalyzed reactions in ILs, (3) laccase-catalyzed reactions, (4) peroxidase-catalyzed reactions, (4) cytochrome-mediated reactions, (5) microbe-mediated hydrations, (6) protease-catalyzed reactions, (8) whole cell mediated asymmetric reduction of ketones, (10) acylase-catalyzed reactions, (11) glycosylation or cellulase-mediated hydrolysis of polysaccharides, (12) hydroxynitrile lyase-catalyzed reaction, (13) fluorinase or haloalkane dehydrogenase-catalyzed reaction, (14) luciferase-catalyzed reactions, and (15) biocatalytic promiscuity of enzymatic reactions for organic synthesis using ILs...
July 26, 2017: Chemical Reviews
https://www.readbyqxmd.com/read/28745311/outer-membrane-vesicle-associated-lipase-ftla-enhances-cellular-invasion-and-virulence-in-francisella-tularensis-lvs
#6
Fei Chen, Guolin Cui, Shuxia Wang, Manoj Kumar Mohan Nair, Lihong He, Xinyi Qi, Xiangmin Han, Hanqi Zhang, Jing-Ren Zhang, Jingliang Su
Francisella tularensis is a highly infectious intracellular pathogen that infects a wide range of host species and causes fatal pneumonic tularemia in humans. ftlA was identified as a potential virulence determinant of the F. tularensis live vaccine strain (LVS) in our previous transposon screen, but its function remained undefined. Here, we show that an unmarked deletion mutant of ftlA was avirulent in a pneumonia mouse model with a severely impaired capacity to infect host cells. Consistent with its sequence homology with GDSL lipase/esterase family proteins, the FtlA protein displayed lipolytic activity in both E...
July 26, 2017: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/28743658/high-level-expression-and-characterization-of-solvent-tolerant-lipase
#7
Yu Wang, Dan Luo, Yunshi Zhao, Shufang Tian, Wenpeng Deng, Chunhua Li, Lixin Ma
In this study, the coding sequence of the lipase from Proteus sp. SW1 was optimized via codon optimization and subjected to expression in Pichia pastoris GS115. The maximum enzyme yield was 387 mg/L in the supernatants of the shake-flask culture. The purified recombinant lipase exhibited a specific activity of 130 U/mg toward p-nitrophenyl Laurate. Its optimum pH and temperature were 8.0 and 40°C, respectively. It was highly stable and even activated in water-miscible solvents, showing over 102% residual activity after 24 h incubation in ethanol, acetone, isopropanol and acetonitrile...
July 22, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28742841/bacillus-sp-jr3-esterase-lipj-a-new-mesophilic-enzyme-showing-traces-of-a-thermophilic-past
#8
Judit Ribera, Mónica Estupiñán, Alba Fuentes, Amanda Fillat, Josefina Martínez, Pilar Diaz
A search for extremophile enzymes from ancient volcanic soils in El Hierro Island (Canary Islands, Spain) allowed isolation of a microbial sporulated strain collection from which several enzymatic activities were tested. Isolates were obtained after sample cultivation under several conditions of nutrient contents and temperature. Among the bacterial isolates, supernatants from the strain designated JR3 displayed high esterase activity at temperatures ranging from 30 to 100°C, suggesting the presence of at least a hyper-thermophilic extracellular lipase...
2017: PloS One
https://www.readbyqxmd.com/read/28742518/severe-hypertriglyceridemia-at-new-onset-type-1-diabetes-mellitus
#9
Tyler Fick, Julie Jack, Amy L Pyle-Eilola, Rohan K Henry
BACKGROUND: Severe hypertriglyceridemia (HTG) as well as diabetic ketoacidosis (DKA) are complications of type 1 diabetes (T1DM). HTG is an exceedingly rare complication in the pediatric population and herein we report a case of HTG at new-onset T1DM in DKA and discuss management and potential complications. CASE PRESENTATION: An 11-year-old previously well patient with a history of fatigue and weight loss presented with: glucose >600 mg/dL, venous blood gas: pH 7...
July 25, 2017: Journal of Pediatric Endocrinology & Metabolism: JPEM
https://www.readbyqxmd.com/read/28742013/first-canadian-experience-with-donation-after-cardiac-death-simultaneous-pancreas-and-kidney-transplants
#10
Patrick T Anderson, Shahid Aquil, Kelly McLean, Vivian C McAlister, Alp Sener, Patrick P Luke
BACKGROUND: Compared with neurologic determination of death (NDD) donor organs, donation after cardiac death (DCD) donor organs have traditionally been considered of inferior quality owing to warm ischemia experienced during procurement. We present, to our knowledge, the first analysis of simultaneous pancreas and kidney (SPK) transplants using DCD donor organs in Canada. METHODS: We carried out a retrospective cohort study of SPK transplants from 13 DCD and 68 NDD donors performed between October 2008 and July 2016...
August 1, 2017: Canadian Journal of Surgery. Journal Canadien de Chirurgie
https://www.readbyqxmd.com/read/28741463/effect-of-organic-solvents-on-porcine-pancreatic-lipase-thermal-aggregation
#11
Mahsa Vaezzadeh, Marjan Sabbaghian, Parichehreh Yaghmaei, Azadeh Ebrahim-Habibi
Proteins tend to form inactive aggregates under harsh conditions used in industrial processes. Lipases are enzymes that hydrolyze triglycerides to glycerol and free fatty acids, but are able to catalyze various other transformations in the presence of organic solvents. Considering this potential, a study on lipases behavior at high temperature and in presence of organic solvents is appropriate. Heat-induced aggregation of porcine pancreatic lipase (PPL) was followed by UV-visible spectroscopy at 400 nm wavelength for 600 seconds, at the isoelectric point (pH 5, phosphate solution) and 50°C, and in presence or absence of various percentages of dimethyl sulfoxide (DMSO), propanol, isopropanol, acetone and trifluoroethanol (TFE)...
July 24, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28741338/fungi-of-the-genus-rhodotorula-isolated-from-the-oral-cavity-of-oncologic-patients-with-colorectal-cancer
#12
Piotr Troska, Ewa Sucharzewska, Maria Dynowska, Elżbieta Ejdys
This paper concerns the frequency of occurrence and enzymatic activity of fungi of the genus Rhodotorula isolated from the oral cavity of patients with colorectal cancer. Swabs from the oral cavity were subjected to standard mycological diagnostic procedures; enzymatic activity of the fungi was evaluated using and API Zym test by BioMereux. The fungi of the genus Rhodotorula were noted in 22.5% positive isolates originating from women and 25.7% positive isolates originating from men. They included mono-species isolates of: Rh...
2017: Annals of Parasitology
https://www.readbyqxmd.com/read/28741302/estimates-of-biologic-variation-in-specific-feline-pancreatic-lipase-concentrations-in-cats-without-clinical-or-ultrasonographic-evidence-of-pancreatitis
#13
Margaret Cohn-Urbach, Craig G Ruaux, Sarah Nemanic
BACKGROUND: Serum concentrations of feline-specific pancreatic lipase are commonly used for diagnosis and monitoring of pancreatitis in cats, but little is known regarding biologic variation of this analyte. OBJECTIVES: The purpose of the study was to determine biologic variation, index of individuality, and reference change values (RCV) for specific feline pancreatic lipase concentrations (Spec fPL) in apparently healthy cats. METHODS: Four blood samples were collected prospectively from 12 apparently healthy cats at 2-week intervals...
July 24, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28741151/probiotic-characteristics-of-lactobacillus-curvatus-dn317-a-strain-isolated-from-chicken-ceca
#14
Mohamed Zommiti, Nathalie Connil, Jeannette Ben Hamida, Mounir Ferchichi
The probiotic characteristics of Lactobacillus curvatus DN317, a strain isolated from chicken ceca, were evaluated. This strain was selected for study from the isolated Lactobacillus strains because it has specific anti-microbial activity against Campylobacter jejuni ATCC 33560, Camp. jejuni NCTC 11168, Listeria monocytogenes ATCC 7644, and Bacillus subtilis ATCC 8633. Lact. curvatus DN317 showed an auto-aggregation percentage of 72% and presented the highest co-aggregation with Lact. monocytogenes ATCC 7644 (68%) compared to B...
July 24, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28740939/effect-of-a-short-period-whole-body-vibration-with-10-hz-on-blood-biomarkers-in-wistar-rats
#15
Milena de Oliveira Bravo Monteiro, Danúbia da Cunha de Sá-Caputo, Eloá Moreira-Marconi, Éric Heleno Freire Ferreira Frederico, Cintia Renata de Sousa-Gonçalves, Luciana Camargo Bernardo, Carlos Alberto Sampaio Guimarães, Mario Bernardo-Filho
BACKGROUND: Exposure to whole body vibration exercises (WBVE), besides some biological effects, causes alterations in the concentration of some blood biomarkers. The aim of this study is to evaluate the action of vibration (10 Hz) of WBVE on the concentration of blood biomarkers in Wistar rats. MATERIALS AND METHODS: Wistar rats were divided in 2 groups. The experimental group (EG) was subjected to vibrations of 10Hz (one min per day, one week, total time of seven min), while the control group (CG) has not experienced vibration...
2017: African Journal of Traditional, Complementary, and Alternative Medicines: AJTCAM
https://www.readbyqxmd.com/read/28739210/bile-salt-stimulated-lipase-inhibition-by-phospholipids-and-relief-by-phospholipase-a2
#16
Elena Venuti, Dmitry Shishmarev, Philip W Kuchel, Shoma Dutt, Caron S Blumenthal, Kevin J Gaskin
INTRODUCTION: Bile salt stimulated lipase (BSSL; Enzyme Commission (EC) number 3.1.1.13) has been a candidate triglyceridase for improving enzyme therapy for pancreatic insufficiency; however, its efficacy is near absent. We hypothesise that similarly to pancreatic lipase, BSSL is inhibited by phospholipids and this inhibition is relieved by Phospholipase A2 (PLA2; EC 3.1.1.4), and the present study was undertaken to explore this possibility. MATERIALS AND METHODS: Synthetic emulsions of triglyceride and phosphatidylcholine (PC) or lysophosphatidylcholine (LPC)/bile salt mixed micelles were used as a model of intestinal digestion-media...
July 21, 2017: Journal of Cystic Fibrosis: Official Journal of the European Cystic Fibrosis Society
https://www.readbyqxmd.com/read/28739185/novel-atherogenic-pathways-from-the-differential-transcriptome-analysis-of-diabetic-epicardial-adipose-tissue
#17
V Camarena, D Sant, M Mohseni, T Salerno, M L Zaleski, G Wang, G Iacobellis
BACKGROUND AND AIM: To evaluate the epicardial adipose tissue (EAT) transcriptome in comparison to subcutaneous fat (SAT) in coronary artery disease (CAD) and type 2 diabetes (T2DM). METHODS AND RESULTS: SAT and EAT samples were obtained from subjects with T2DM and CAD (n = 5) and those without CAD with or without T2DM (=3) undergoing elective cardiac surgery. RNA-sequencing analysis was performed in both EAT and SAT. Gene enrichment analysis was conducted to identify pathways affected by the differentially expressed genes...
June 10, 2017: Nutrition, Metabolism, and Cardiovascular Diseases: NMCD
https://www.readbyqxmd.com/read/28735047/sesamol-exhibits-potent-antimycobacterial-activity-underlying-mechanisms-and-impact-on-virulence-traits
#18
Sandeep Hans, Sharda Sharma, Saif Hameed, Zeeshan Fatima
OBJECTIVES: Novel strategies to overcome multidrug resistance (MDR) in Tuberculosis (TB) still remain a concern. Usage of natural compounds nowadays to surmount the increasing burden of MDR-TB has shown promising results. The aim of this study was to evaluate the antimycobacterial potential of sesamol (Ses) a natural phenolic compound against Mycobacterium smegmatis, a surrogate for MTB and its underlying mechanism of action along with its effect on mycobacterial virulence traits. METHODS: Cell surface phenotypes were estimated microscopically and spectrophotometrically respectively...
July 19, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28734759/nivolumab-in-patients-with-metastatic-dna-mismatch-repair-deficient-or-microsatellite-instability-high-colorectal-cancer-checkmate-142-an-open-label-multicentre-phase-2-study
#19
Michael J Overman, Ray McDermott, Joseph L Leach, Sara Lonardi, Heinz-Josef Lenz, Michael A Morse, Jayesh Desai, Andrew Hill, Michael Axelson, Rebecca A Moss, Monica V Goldberg, Z Alexander Cao, Jean-Marie Ledeine, Gregory A Maglinte, Scott Kopetz, Thierry André
BACKGROUND: Metastatic DNA mismatch repair-deficient (dMMR)/microsatellite instability-high (MSI-H) colorectal cancer has a poor prognosis after treatment with conventional chemotherapy and exhibits high levels of tumour neoantigens, tumour-infiltrating lymphocytes, and checkpoint regulators. All of these features are associated with the response to PD-1 blockade in other tumour types. Therefore, we aimed to study nivolumab, a PD-1 immune checkpoint inhibitor, in patients with dMMR/MSI-H metastatic colorectal cancer...
July 19, 2017: Lancet Oncology
https://www.readbyqxmd.com/read/28733934/draft-genome-sequence-of-the-deep-sea-bacterium-moritella-sp-jt01-and-identification-of-biotechnologically-relevant-genes
#20
Robert Cardoso de Freitas, Estácio Jussie Odisi, Chiaki Kato, Marcus Adonai Castro da Silva, André Oliveira de Souza Lima
Deep-sea bacteria can produce various biotechnologically relevant enzymes due to their adaptations to high pressures and low temperatures. To identify such enzymes, we have sequenced the genome of the polycaprolactone-degrading bacterium Moritella sp. JT01, isolated from sediment samples from Japan Trench (6957 m depth), using a Illumina HiSeq2000 sequencer (12.1 million paired-end reads) and CLC Genomics Workbench (version 6.5.1) for the assembly, resulting in a 4.83-Mb genome (42 scaffolds). The genome was annotated using Rapid Annotation using Subsystem Technology (RAST), Protein Homology/analogY Recognition Engine V 2...
July 22, 2017: Marine Biotechnology
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