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IFN-gamma signaling

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https://www.readbyqxmd.com/read/29278364/tnf-%C3%AE-and-ifn-%C3%AE-together-up-regulates-par-4-expression-and-induce-apoptosis-in-human-neuroblastomas
#1
Ganesh V Shelke, Jayashree C Jagtap, Dae-Kyum Kim, Reecha D Shah, Gowry Das, Mruthyunjaya Shivayogi, Radha Pujari, Padma Shastry
The objective of this study was to examine the combined effect of Interferon-gamma (IFN-γ) and Tumor Necrosis factor-alpha (TNF-α) on cytotoxicity and expression of prostate apoptosis response-4 (Par-4) and Par-4 interacting proteins B-cell lymphoma (Bcl-2), nuclear factor kappa-light-chain-enhancer of activated B cells/p65 subunit (NF-κB/p65), Ak mouse strain thymoma (Akt) in human neuroblastoma (NB) cells. Materials and methods included human neuroblastoma cell lines-SK-N-MC, SK-N-SH, and SH-SY5Y, which were treated with IFN-γ and TNF-α individually, or in combination, and were assessed for viability by tetrazolium (MTT) assay...
December 26, 2017: Biomedicines
https://www.readbyqxmd.com/read/29275861/natural-killer-cells-control-tumor-growth-by-sensing-a-growth-factor
#2
Alexander D Barrow, Melissa A Edeling, Vladimir Trifonov, Jingqin Luo, Piyush Goyal, Benjamin Bohl, Jennifer K Bando, Albert H Kim, John Walker, Mary Andahazy, Mattia Bugatti, Laura Melocchi, William Vermi, Daved H Fremont, Sarah Cox, Marina Cella, Christian Schmedt, Marco Colonna
Many tumors produce platelet-derived growth factor (PDGF)-DD, which promotes cellular proliferation, epithelial-mesenchymal transition, stromal reaction, and angiogenesis through autocrine and paracrine PDGFRβ signaling. By screening a secretome library, we found that the human immunoreceptor NKp44, encoded by NCR2 and expressed on natural killer (NK) cells and innate lymphoid cells, recognizes PDGF-DD. PDGF-DD engagement of NKp44 triggered NK cell secretion of interferon gamma (IFN)-γ and tumor necrosis factor alpha (TNF-α) that induced tumor cell growth arrest...
December 20, 2017: Cell
https://www.readbyqxmd.com/read/29261164/lipopolysaccharide-induced-acute-kidney-injury-is-dependent-on-an-il-18-receptor-signaling-pathway
#3
Yuji Nozaki, Shoichi Hino, Jinhai Ri, Kenji Sakai, Yasuaki Nagare, Mai Kawanishi, Kaoru Niki, Masanori Funauchi, Itaru Matsumura
The proinflammatory cytokine interleukin (IL)-18 is an important mediator of the organ failure induced by endotoxemia. IL-18 (known as an interferon-gamma (IFN-γ) inducing factor), and other inflammatory cytokines have important roles in lipopolysaccharide (LPS)-induced acute kidney injury (AKI). We investigated the effect of inflammatory cytokines and Toll-like receptor 4 (TLR4) expression, an event that is accompanied by an influx of monocytes, including CD4⁺ T cells and antigen-presenting cells (APCs) in IL-18Rα knockout (KO) mice and wild-type (WT) mice after LPS injection...
December 20, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29250063/m-il-4-tissue-macrophages-support-efficient-interferon-gamma-production-in-antigen-specific-cd8-t-cells-with-reduced-proliferative-capacity
#4
Rylend Mulder, Andra Banete, Kyle Seaver, Sameh Basta
CD8+ cytotoxic T cell (CTL) responses are necessary for the lysis of virally infected cells and control of infection. CTLs are activated when their TCRs bind a major histocompatibility complex (MHC)-I/peptide complex on the surface of antigen presenting cells such as macrophages (MΦ). It is now apparent that MΦ display remarkable plasticity in response to environmental signals to polarize into classically activated M(LPS + IFN-γ) or alternatively activated M(IL-4). However, little is known about how MΦ activation status influences their antigen presentation function to CD8+ T cell in models of virus infection...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29249315/the-immunomodulatory-function-of-equine-mscs-is-enhanced-by-priming-through-an-inflammatory-microenvironment-or-tlr3-ligand
#5
Jennifer M Cassano, Lauren V Schnabel, Margaret B Goodale, Lisa A Fortier
Mesenchymal stem cells (MSCs) have the therapeutic potential to treat a variety of inflammatory and degenerative disease processes, however the effects of the tissue environment on MSCs have been overlooked. Our hypothesis was that the immunomodulatory function of MSCs would be impaired by TLR4 stimulation or exposure to inflammatory macrophages, whereas their immunosuppressive properties would be enhanced by TLR3 stimulation. MSCs were exposed to polyinosinic:polycytidylic acid (poly I:C) to stimulate TLR3 receptors or lipopolysaccharide (LPS) to stimulate TLR4 receptors...
January 2018: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/29230805/uspio-labeling-in-m1-and-m2-polarized-macrophages-an-in-vitro-study-using-a-clinical-magnetic-resonance-scanner
#6
Chiara Zini, Mary Anna Venneri, Selenia Miglietta, Damiano Caruso, Natale Porta, Andrea Isidori, Daniela Fiore, Daniele Gianfrilli, Vincenzo Petrozza, Andrea Laghi
BACKGROUND: To evaluate USPIO labeling in different macrophage populations using a clinical 3.0T MR unit with optical and electron microscopy as the gold standard. METHODS: Human monocytic cell line were differentiated into macrophages. Afterwards M0 macrophages were incubated with IL-4 and IL-13 in order to obtain M2 polarized macrophages or with IFN-gamma and LPS for classical macrophage activation (M1). These groups were incubated with USPIO MR contrast agent (P904) for 36 hours; M0, M0+P904, M1+P904 and M2+P904 were analyzed in gel phantoms with a 3...
December 12, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29218573/dual-neutralization-of-tnfr-2-and-mmp-2-regulates-the-severity-of-s-aureus-induced-septic-arthritis-correlating-alteration-in-the-level-of-interferon-gamma-and-interleukin-10-in-terms-of-tnfr2-blocking
#7
Sahin Sultana, Rajen Dey, Biswadev Bishayi
Severity of S. aureus septic arthritis is correlated to prolonged inflammation by inflammatory cytokines like TNF-α, IL-1β, and IL-6 even after successful elimination of bacteria. Role of TNF-α via TNFR2 is not well established in this aspect. IFN-γ induces TNF-α release from the macrophages augmenting the inflammatory arthritis. IL-10 modulates the levels of pro-inflammatory cytokines promoting resolution of inflammation. TNF-α-TNFR2 signaling upregulates both of these cytokines. Higher level of MMP-2 induction by inflammatory cytokines during arthritis promotes tissue destruction...
December 7, 2017: Immunologic Research
https://www.readbyqxmd.com/read/29187651/-comparison-of-effects-between-il-2-and-il-7-on-glutamic-acid-decarboxylase-65-reactive-t-cell-responses-in-patients-with-type-1-diabetes
#8
Jiao Yuan, Qiuyan Cui, Wei Tang, Chen Chao, Zhiguang Zhou, Lin Yang
To explore the type of cytokine (IL-2 or IL-7) and its most optimal concentration regarding the improvement of the signal-to-noise ratio of glutamic acid decarboxylase 65 (GAD65) in enzyme-linked immunospot (ELISPOT) assay in Type 1 diabetic (T1DM) patients.
 Methods: Twenty T1DM patients (Group A) and sixteen healthy controls matched with age and sex (Group B) were enrolled in our study, and their peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll method. GAD65, internal control and Pediacel served as "five-for-one" vaccine were selected as the stimulating antigen...
November 28, 2017: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/29187449/high-stat4-expression-indicates-better-disease-free-survival-in-patients-with-gastric-cancer
#9
Masaaki Nishi, Bat-Erdene Batsaikhan, Kozo Yoshikawa, Jun Higashijima, Takuya Tokunaga, Chie Takasu, Hideya Kashihara, Daichi Ishikawa, Mitsuo Shimada
BACKGROUND: The aim of this study was to investigate the significance of signal transducer and activator of transcription 4 (STAT4) expression and the correlation between STAT4 and interferon gamma (IFN-γ) in patients with gastric cancer. PATIENTS AND METHODS: Sixty-two patients who underwent gastrectomy for gastric cancer were enrolled in the study. STAT4 and IFNG mRNA expression was evaluated by quantitative real-time polymerase chain reaction (PCR). Immunohistochemistry was performed to examine CD8+ T-cells, and STAT4 and IFN-γ expression...
December 2017: Anticancer Research
https://www.readbyqxmd.com/read/29143896/modulation-of-stimulator-of-interferon-genes-sting-expression-by-interferon-%C3%AE-in-human-keratinocytes
#10
Yohei Nishikawa, Yasushi Matsuzaki, Kazuyuki Kimura, Akiko Rokunohe, Hajime Nakano, Daisuke Sawamura
Infection of microbial pathogen triggers the innate immune system, and the induction of interferons (IFNs) play a vital role in host antiviral response. Stimulator of interferon genes (STING) was identified as a crucial regulator of the DNA sensing pathway, and activates both nuclear factor-κB and interferon regulatory factor 3 transcription pathways to evoke IFNs production. In this study, we studied the upregulation of STING mRNA expression, induced by IFN-γ in human keratinocytes (HaCaT). STING promoter assays clarified that a gamma-activated sequence (GAS), located at - 7 to - 15-bp, is required for IFN-γ-upregulated promoter activity...
November 16, 2017: Biochemical Genetics
https://www.readbyqxmd.com/read/29126802/gamma-irradiated-black-ginseng-extract-inhibits-mast-cell-degranulation-and-suppresses-atopic-dermatitis-like-skin-lesions-in-mice
#11
Jung Ae Kang, Ha-Yeon Song, Eui-Hong Byun, Nam-Geun Ahn, Hye-Min Kim, You Ree Nam, Gyeong Hee Lee, Beom-Su Jang, Dae Seong Choi, Dong-Eun Lee, Eui-Baek Byun
Gamma irradiation is able to affect various structural modification and an increase of the biological properties of biomaterials. This study was conducted to investigate the anti-allergenic effect of γ-irradiated black ginseng extract (BGE) using in vitro and in vivo experiments. IgEantigen complex-induced degranulation was measured in RBL-2H3 mast cells. In addition, an anti-atopic dermatitis (AD) test was carried out by spreading γ-irradiated BGE on the dorsal skin of 2,4-dinitrochlorobenzene (DNCB)-induced BALB/c mice...
November 7, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29113698/the-control-of-oligodendrocyte-bioenergetics-by-interferon-gamma-ifn-%C3%AE-and-src-homology-region-2-domain-containing-phosphatase-1-shp-1
#12
Scott B Minchenberg, Paul T Massa
Glycolysis and mitochondrial respiration are essential for oligodendrocyte metabolism in both the developing and adult CNS. Based on recent reports on the effects of the proinflammatory cytokine IFN-γ on metabolism and on oligodendrocytes, we addressed whether IFN-γ may affect oligodendrocyte bioenergetics in ways relevant to CNS disease. Oligodendrocytes of mice treated with IFN-γ showed significant reductions in aerobic glycolysis and mitochondrial respiration. As expected, IFN-γ treatment led to the induction of STAT1 in oligodendrocytes indicating active signaling into these cells...
October 28, 2017: Journal of Neuroimmunology
https://www.readbyqxmd.com/read/29110394/methylisothiazolinone-may-induce-cell-death-and-inflammatory-response-through-dna-damage-in-human-liver-epithelium-cells
#13
Eun-Jung Park, Sanghwa Kim, Jaerak Chang
Methylisothiazolinone (MIT) is a powerful biocide and preservative, which is widely used alone or in a 1:3 ratio with methylchloroisothiazolinone (MCIT) under the trade name of Kathons in the manufacture of numerous personal and household products. Considering that Kathons injected intravenously is distributed in the blood and then in the liver, we explored the toxic mechanism of MIT on human liver epithelium cells. At 24 h after exposure, MIT bound to the plasma membrane and the inner wall of vacuoles in the cells, and rupture of the cell membrane and nuclear envelop, autophagosome-like vacuoles formation and mitochondrial damage were observed...
November 7, 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/29109772/the-anti-tumor-effects-of-m1-macrophage-loaded-poly-ethylene-glycol-and-gelatin-based-hydrogels-on-hepatocellular-carcinoma
#14
Alberto Daniel Guerra, Oscar W H Yeung, Xiang Qi, W John Kao, Kwan Man
Background and Aims: Recently we reported that direct injection of M1 macrophages significantly caused tumor regression in vivo. Despite the promising result, a major limitation in translating this approach is the induction of acute inflammatory response. To improve the strategy, a biocompatible scaffold for cell presentation and support is essential to control cell fate. Here, we aimed to elucidate the anti-tumor effects of a poly(ethylene glycol) diacrylate (PEGdA) and thiolated gelatin poly(ethylene glycol) (Gel-PEG-Cys) cross-linked hydrogels capsulated with M1 macrophages in both in vitro and in vivo disease models...
2017: Theranostics
https://www.readbyqxmd.com/read/29100332/the-farnesoid-x-receptor-negatively-regulates-osteoclastogenesis-in-bone-remodeling-and-pathological-bone-loss
#15
Ting Zheng, Ju-Hee Kang, Jung-Sun Sim, Jung-Woo Kim, Jeong-Tae Koh, Chan Soo Shin, Hyungsik Lim, Mijung Yim
Farnesoid X receptor (FXR, NR1H4) is a member of the nuclear receptor superfamily of ligand-activated transcription factors. Since the role of FXR in osteoclast differentiation remains ill-defined, we investigated the biological function of FXR on osteoclastogenesis, using FXR-deficient mice. We demonstrated that FXR deficiency increases osteoclast formation in vitro and in vivo. First, FXR deficiency was found to accelerate osteoclast formation via down-regulation of c-Jun N-terminal kinase (JNK) 1/2 expression...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29100036/nafamostat-mesilate-a-serine-protease-inhibitor-suppresses-interferon-gamma-induced-up-regulation-of-programmed-cell-death-ligand-1-in-human-cancer-cells
#16
Sadamu Homma, Kazumi Hayashi, Kosaku Yoshida, Yukiko Sagawa, Yuko Kamata, Masaki Ito
Programmed cell death ligand-1 (PD-L1) plays a pivotal role in the suppression of antitumour immunity by binding to programmed cell death-1 (PD-1) on tumouricidal cytotoxic T lymphocytes (CTLs), rendering them inactive. As blockade of PD-1/PD-L1 interaction by the monoclonal antibodies induced effective T cell-mediated antitumour response, suppression of PD-L1 expression in tumour cells by the chemical agent might contribute to treatment against malignant tumours. Nafamostat mesilate (NM), a serine protease inhibitor that is frequently used in the clinic, potently suppressed interferon-gamma (IFN-gamma)-induced up-regulation of PD-L1 in cultured human lung cancer cells (HLC-1) at both the messenger RNA (mRNA) and protein levels...
January 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29036979/-role-of-ash2-absent-small-or-homeotic-like-and-jumonji-domain-containing-protein-3-on-histone-methylation-of-interferon-gamma-gene-and-their-associations-with-vascular-damage-of-kawasaki-disease
#17
J H Mei, G Tang, Q Wang, P Q Wen, M G Xu, D Cui, D L Ma, C Liu, G B Wang
Objective: To investigate the impacts of ash2 (absent, small, or homeotic)-like (Ash2L) and Jumonji domain-containing protein 3 (Jmjd3) on histone methylation of interferon-gamma(IFN-γ) gene and association with vascular damage of Kawasaki disease (KD) in acute phase. Methods: This study was performed among 36 children with KD in acute phase (KD group) and 28 age-matched health children (control group), who were treated or underwent physical examination in our hospital between February 2015 and June 2016. Patients were further divided into KD groups with or without coronary artery lesions (KD-CAL(+) , 16 cases; KD-CAL(-), 20 cases)...
September 24, 2017: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/29034543/pd-l1-expression-is-mainly-regulated-by-interferon-gamma-associated-with-jak-stat-pathway-in-gastric-cancer
#18
Kousaku Mimura, Jun Liang Teh, Hirokazu Okayama, Kensuke Shiraishi, Ley-Fang Kua, Vivien Koh, Duane T Smoot, Hassan Ashktorab, Takahiro Oike, Yoshiyuki Suzuki, Zul Fazreen, Bernadette R Asuncion, Asim Shabbir, Wei-Peng Yong, Jimmy So, Richie Soong, Koji Kono
Despite multidisciplinary treatment for patients with advanced gastric cancer, their prognosis remains poor. Therefore, the development of novel therapeutic strategies is urgently needed, and immunotherapy utilizing anti-programmed death 1/-programmed death ligand-1 mAb is an attractive approach. However, as there is limited information on how programmed death ligand-1 is upregulated on tumor cells within the tumor microenvironment, we examined the mechanism of programmed death ligand-1 regulation with a particular focus on interferon gamma in an in vitro setting and in clinical samples...
October 16, 2017: Cancer Science
https://www.readbyqxmd.com/read/29033545/downregulation-of-a20-increases-the-cytotoxicity-of-ifn-%C3%AE-in-hepatocellular-carcinoma-cells
#19
Lei Yin, Zheng Fang, Ning-Jia Shen, Ying-He Qiu, Ai-Jun Li, Yong-Jie Zhang
Hepatocellular carcinoma (HCC) is a highly fatal disease mandating development of novel, effective therapeutic strategy. Interferon-gamma (IFN-γ) is a pleiotropic cytokine with immunomodulatory, antiviral, and antitumor effects. Although IFN-γ is a promising antitumor agent, its application is limited by resistance in tumor cells. A20 is a zinc-finger protein that was initially identified as a gene product induced by tumor necrosis factor α in human umbilical vein endothelial cells. In this study, we found that silencing of A20 combined with IFN-γ significantly represses cell viability, and induces apoptosis and cell-cycle arrest in HCC cells...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/28968560/human-b-cells-fail-to-secrete-type-i-interferons-upon-cytoplasmic-dna-exposure
#20
Anna M Gram, Chenglong Sun, Sanne L Landman, Timo Oosenbrug, Hester J Koppejan, Mark J Kwakkenbos, Rob C Hoeben, Søren R Paludan, Maaike E Ressing
Most cells are believed to be capable of producing type I interferons (IFN I) as part of an innate immune response against, for instance, viral infections. In macrophages, IFN I is potently induced upon cytoplasmic exposure to foreign nucleic acids. Infection of these cells with herpesviruses leads to triggering of the DNA sensors interferon-inducible protein 16 (IFI16) and cyclic GMP-AMP (cGAMP) synthase (cGAS). Thereby, the stimulator of interferon genes (STING) and the downstream molecules TANK-binding kinase 1 (TBK1) and interferon regulatory factor 3 (IRF3) are sequentially activated culminating in IFN I secretion...
November 2017: Molecular Immunology
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