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https://www.readbyqxmd.com/read/29772511/estrogenic-potency-of-mc-lr-is-induced-via-stimulating-steroidogenesis-in-vitro-and-in-vivo-evidence
#1
Jie Hou, Yujing Su, Wang Lin, Honghui Guo, Li Li, Donald M Anderson, Dapeng Li, Rong Tang, Wei Chi, Xi Zhang
Waterborne microcystin-LR (MC-LR) has been reported to disrupt sex hormones, while its estrogenic potency remains controversial. We hypothesized that MC-LR could induce estrogenic effects via disrupting sex hormone synthesis, and verified this hypothesis by in vitro and in vivo assays. Effects of MC-LR (1, 10, 100, 500, 1000 and 5000 μg/L) on steroidogenesis were assessed in the H295R cells after 48 h. The contents of 17β-estradiol (E2) and testosterone (T) increased in a non-dose-dependent manner, which showed positive correlations with the expression of steroidogenic genes...
May 14, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29768214/noise-in-the-vertebrate-segmentation-clock-is-boosted-by-time-delays-but-tamed-by-notch-signaling
#2
Sevdenur Keskin, Gnanapackiam S Devakanmalai, Soo Bin Kwon, Ha T Vu, Qiyuan Hong, Yin Yeng Lee, Mohammad Soltani, Abhyudai Singh, Ahmet Ay, Ertuğrul M Özbudak
Taming cell-to-cell variability in gene expression is critical for precise pattern formation during embryonic development. To investigate the source and buffering mechanism of expression variability, we studied a biological clock, the vertebrate segmentation clock, controlling the precise spatiotemporal patterning of the vertebral column. By counting single transcripts of segmentation clock genes in zebrafish, we show that clock genes have low RNA amplitudes and expression variability is primarily driven by gene extrinsic sources, which is suppressed by Notch signaling...
May 15, 2018: Cell Reports
https://www.readbyqxmd.com/read/29751160/interactions-of-oxidized-multiwalled-carbon-nanotube-with-cadmium-on-zebrafish-cell-line-the-influence-of-two-co-exposure-protocols-on-in-vitro-toxicity-tests
#3
Mariana Morozesk, Lidiane S Franqui, Adrislaine S Mansano, Diego Stéfani T Martinez, Marisa N Fernandes
The widespread production and application of carbon nanotubes (CNT) have raising concerns about their release into the environment and, the joint toxicity of CNT with pre-existing contaminants needs to be assessed. This is the first study that investigated the co-exposure of oxidized multiwalled carbon nanotubes (ox-MWCNT) and cadmium (Cd) using a zebrafish liver cell line (ZFL). Two in vitro co-exposure protocols differing by the order of ox-MWCNT interaction with Cd and fetal bovine serum (FBS) proteins were evaluated...
May 5, 2018: Aquatic Toxicology
https://www.readbyqxmd.com/read/29743725/ufd1-contributes-to-myc-mediated-leukemia-aggressiveness-through-suppression-of-the-proapoptotic-unfolded-protein-response
#4
L N Huiting, Y Samaha, G L Zhang, J E Roderick, B Li, N M Anderson, Y W Wang, L Wang, Fjf Laroche, J W Choi, C T Liu, M A Kelliher, H Feng
Despite the pivotal role of MYC in tumorigenesis, the mechanisms by which it promotes cancer aggressiveness remain incompletely understood. Here, we show that MYC transcriptionally upregulates the ubiquitin fusion degradation 1 (UFD1) gene in T-cell acute lymphoblastic leukemia (T-ALL). Allelic loss of ufd1 in zebrafish induces tumor cell apoptosis and impairs MYC-driven T-ALL progression but does not affect general health. As the E2 component of an endoplasmic reticulum (ER)-associated degradation (ERAD) complex, UFD1 facilitates the elimination of misfolded/unfolded proteins from the ER...
April 25, 2018: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
https://www.readbyqxmd.com/read/29735715/znrf3-functions-in-mammalian-sex-determination-by-inhibiting-canonical-wnt-signaling
#5
Abigail Harris, Pam Siggers, Silvia Corrochano, Nick Warr, Danielle Sagar, Daniel T Grimes, Makoto Suzuki, Rebecca D Burdine, Feng Cong, Bon-Kyoung Koo, Hans Clevers, Isabelle Stévant, Serge Nef, Sara Wells, Raja Brauner, Bochra Ben Rhouma, Neïla Belguith, Caroline Eozenou, Joelle Bignon-Topalovic, Anu Bashamboo, Ken McElreavey, Andy Greenfield
Mammalian sex determination is controlled by the antagonistic interactions of two genetic pathways: The SRY-SOX9-FGF9 network promotes testis determination partly by opposing proovarian pathways, while RSPO1/WNT-β-catenin/FOXL2 signals control ovary development by inhibiting SRY-SOX9-FGF9. The molecular basis of this mutual antagonism is unclear. Here we show that ZNRF3, a WNT signaling antagonist and direct target of RSPO1-mediated inhibition, is required for sex determination in mice. XY mice lacking ZNRF3 exhibit complete or partial gonadal sex reversal, or related defects...
May 7, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29722096/the-14-th-international-workshops-on-opportunistic-protists-iwop-14
#6
Melanie T Cushion, Andrew H Limper, Aleksey Porollo, Vivian Saper, Anthony P Sinai, Louis M Weiss
The 14th International Workshops on Opportunistic Protists (IWOP-14) was held August 10 to 12, 2017 in Cincinnati, OH, USA. The IWOP meetings focus on opportunistic protists (OIs); e.g. free-living amoebae, Pneumocystis, Cryptosporidium, Toxoplasma, the Microsporidia, and kinetoplastid flagellates. Highlights of Pneumocystis research included reports of primary homothallism for mating; a potential requirement for sexual replication in its life cycle; a new antigen on the surface of small asci; and roles for CLRs, Dectin-1 and Mincle in host responses; and identification of MSG families and mechanisms used for surface variation...
May 3, 2018: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/29679867/n-acetylcysteine-protects-against-microcystin-lr-induced-endoplasmic-reticulum-stress-and-germ-cell-apoptosis-in-zebrafish-testes
#7
Sujuan Zhao, Ying Liu, Fang Wang, Dexiang Xu, Ping Xie
Previous studies have shown that microcystin-LR (MCLR) is a reproductive toxicant that induces germ cell apoptosis in the testes, but the underlying mechanisms have not been well understood. In this study, we investigated that MCLR induces germ cell apoptosis is through activation of endoplasmic reticulum (ER) stress and N-acetylcysteine (NAC), an antioxidant could protect against germ cell apoptosis by inhibiting the ER stress. Healthy male zebrafish were intraperitoneally injected with NAC (500 nM), beginning at 2 h before different doses of MCLR (0, 50, 100, 200 μg/kg)...
August 2018: Chemosphere
https://www.readbyqxmd.com/read/29672531/embryominer-a-new-framework-for-interactive-knowledge-discovery-in-large-scale-cell-tracking-data-of-developing-embryos
#8
Benjamin Schott, Manuel Traub, Cornelia Schlagenhauf, Masanari Takamiya, Thomas Antritter, Andreas Bartschat, Katharina Löffler, Denis Blessing, Jens C Otte, Andrei Y Kobitski, G Ulrich Nienhaus, Uwe Strähle, Ralf Mikut, Johannes Stegmaier
State-of-the-art light-sheet and confocal microscopes allow recording of entire embryos in 3D and over time (3D+t) for many hours. Fluorescently labeled structures can be segmented and tracked automatically in these terabyte-scale 3D+t images, resulting in thousands of cell migration trajectories that provide detailed insights to large-scale tissue reorganization at the cellular level. Here we present EmbryoMiner, a new interactive open-source framework suitable for in-depth analyses and comparisons of entire embryos, including an extensive set of trajectory features...
April 19, 2018: PLoS Computational Biology
https://www.readbyqxmd.com/read/29615667/lipoprotein-lipase-regulates-hematopoietic-stem-progenitor-cell-maintenance-through-dha-supply
#9
Chao Liu, Tianxu Han, David L Stachura, Huawei Wang, Boris L Vaisman, Jungsu Kim, Richard L Klemke, Alan T Remaley, Tariq M Rana, David Traver, Yury I Miller
Lipoprotein lipase (LPL) mediates hydrolysis of triglycerides (TGs) to supply free fatty acids (FFAs) to tissues. Here, we show that LPL activity is also required for hematopoietic stem progenitor cell (HSPC) maintenance. Knockout of Lpl or its obligatory cofactor Apoc2 results in significantly reduced HSPC expansion during definitive hematopoiesis in zebrafish. A human APOC2 mimetic peptide or the human very low-density lipoprotein, which carries APOC2, rescues the phenotype in apoc2 but not in lpl mutant zebrafish...
April 3, 2018: Nature Communications
https://www.readbyqxmd.com/read/29615555/imaging-neuronal-activity-in-the-optic-tectum-of-late-stage-larval-zebrafish
#10
Katharina Bergmann, Paola Meza Santoscoy, Konstantinos Lygdas, Yulia Nikolaeva, Ryan B MacDonald, Vincent T Cunliffe, Anton Nikolaev
The zebrafish is an established model to study the development and function of visual neuronal circuits in vivo, largely due to their optical accessibility at embryonic and larval stages. In the past decade multiple experimental paradigms have been developed to study visually-driven behaviours, particularly those regulated by the optic tectum, the main visual centre in lower vertebrates. With few exceptions these techniques are limited to young larvae (7-9 days post-fertilisation, dpf). However, many forms of visually-driven behaviour, such as shoaling, emerge at later developmental stages...
March 9, 2018: Journal of Developmental Biology
https://www.readbyqxmd.com/read/29601828/identification-of-variants-in-ret-and-ihh-pathway-members-in-a-large-family-with-history-of-hirschsprung-disease
#11
Y Sribudiani, R K Chauhan, M M Alves, L Petrova, E Brosens, C Harrison, T Wabbersen, B M de Graaf, T Rügenbrink, G Burzynski, R W W Brouwer, W F J van IJcken, S M Maas, A de Klein, J Osinga, B J L Eggen, A J Burns, A S Brooks, I T Shepherd, R M W Hofstra
BACKGROUND & AIMS: Hirschsprung disease (HSCR) is an inherited congenital disorder characterized by absence of enteric ganglia in the distal part of the gut. Variants in ret proto-oncogene (RET) have been associated with up to 50% of familial and 35% of sporadic cases. We searched for variants that affect disease risk in a large, multi-generational family with history of HSCR in a linkage region previously associated with the disease (4q31.3-q32.3) and exome wide. METHODS: We performed exome sequencing analyses of a family in the Netherlands with 5 members diagnosed with HSCR and 2 members diagnosed with functional constipation...
March 27, 2018: Gastroenterology
https://www.readbyqxmd.com/read/29590635/identification-of-protective-postexposure-mycobacterial-vaccine-antigens-using-an-immunosuppression-based-reactivation-model-in-the-zebrafish
#12
Henna Myllymäki, Mirja Niskanen, Hanna Luukinen, Mataleena Parikka, Mika Rämet
Roughly one third of the human population carries a latent Mycobacterium tuberculosis infection, with a 5-10% lifetime risk of reactivation to active tuberculosis and further spreading the disease. The mechanisms leading to the reactivation of a latent Mycobacterium tuberculosis infection are insufficiently understood. Here, we used a natural fish pathogen, Mycobacterium marinum , to model the reactivation of a mycobacterial infection in the adult zebrafish ( Danio rerio ). A low-dose intraperitoneal injection (∼40 colony-forming units) led to a latent infection, with mycobacteria found in well-organized granulomas surrounded by a thick layer of fibrous tissue...
March 13, 2018: Disease Models & Mechanisms
https://www.readbyqxmd.com/read/29560280/micro-rnas-are-involved-in-activation-of-epicardium-during-zebrafish-heart-regeneration
#13
Marcello Ceci, Claudia Carlantoni, Maria Azzurra Missinato, Davide Bonvissuto, Bruna Di Giacomo, Riccardo Contu, Nicla Romano
Zebrafish could be an interesting translational model to understand and improve the post-infarction trial and possible regeneration in humans. The adult zebrafish is able to regenerate efficiently after resecting nearly 20% of the ventricular apex. This process requires the concert activation of the epicardium and endocardium, as well as trans-differentiation of pre-existing cardiomyocytes that together replace the lost tissue. The molecular mechanisms involved in this activation process are not completely clarified...
2018: Cell Death Discovery
https://www.readbyqxmd.com/read/29550364/mir-126-is-a-conserved-modulator-of-lymphatic-development
#14
Zacharias Kontarakis, Andrea Rossi, Sophie Ramas, Michael T Dellinger, Didier Y R Stainier
Organ homeostasis relies upon cellular and molecular processes that restore tissue structure and function in a timely fashion. Lymphatic vessels help maintain fluid equilibrium by returning interstitial fluid that evades venous uptake back to the circulation. Despite its important role in tissue homeostasis, cancer metastasis, and close developmental origins with the blood vasculature, the number of molecular players known to control lymphatic system development is relatively low. Here we show, using genetic approaches in zebrafish and mice, that the endothelial specific microRNA mir-126, previously implicated in vascular integrity, regulates lymphatic development...
May 15, 2018: Developmental Biology
https://www.readbyqxmd.com/read/29546880/cell-motility-in-cancer-invasion-and-metastasis-insights-from-simple-model-organisms
#15
REVIEW
Christina H Stuelten, Carole A Parent, Denise J Montell
Metastasis remains the greatest challenge in the clinical management of cancer. Cell motility is a fundamental and ancient cellular behaviour that contributes to metastasis and is conserved in simple organisms. In this Review, we evaluate insights relevant to human cancer that are derived from the study of cell motility in non-mammalian model organisms. Dictyostelium discoideum, Caenorhabditis elegans, Drosophila melanogaster and Danio rerio permit direct observation of cells moving in complex native environments and lend themselves to large-scale genetic and pharmacological screening...
May 2018: Nature Reviews. Cancer
https://www.readbyqxmd.com/read/29540787/microvillar-and-ciliary-defects-in-zebrafish-lacking-an-actin-binding-bioactive-peptide-amidating-enzyme
#16
Dhivya Kumar, Rebecca T Thomason, Maya Yankova, Jonathan D Gitlin, Richard E Mains, Betty A Eipper, Stephen M King
The assembly of membranous extensions such as microvilli and cilia in polarized cells is a tightly regulated, yet poorly understood, process. Peptidylglycine α-amidating monooxygenase (PAM), a membrane enzyme essential for the synthesis of amidated bioactive peptides, was recently identified in motile and non-motile (primary) cilia and has an essential role in ciliogenesis in Chlamydomonas, Schmidtea and mouse. In mammalian cells, changes in PAM levels alter secretion and organization of the actin cytoskeleton...
March 14, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29540468/genetic-reduction-in-left-ventricular-protein-kinase-c-%C3%AE-and-adverse-ventricular-remodeling-in-human-subjects
#17
Ray Hu, Michael P Morley, Jeffrey Brandimarto, Nathan R Tucker, Victoria A Parsons, Sihai D Zhao, Benjamin Meder, Hugo A Katus, Frank Rühle, Monika Stoll, Eric Villard, François Cambien, Honghuang Lin, Nicholas L Smith, Janine F Felix, Ramachandran S Vasan, Pim van der Harst, Christopher Newton-Cheh, Jin Li, Cecilia E Kim, Hakon Hakonarson, Sridhar Hannenhalli, Euan A Ashley, Christine S Moravec, W H Wilson Tang, Marjorie Maillet, Jeffery D Molkentin, Patrick T Ellinor, Kenneth B Margulies, Thomas P Cappola
BACKGROUND: Inhibition of PKC-α (protein kinase C-α) enhances contractility and cardioprotection in animal models, but effects in humans are unknown. Genotypes at rs9912468 strongly associate with PRKCA expression in the left ventricle, enabling genetic approaches to measure effects of reduced PKC-α in human populations. METHODS AND RESULTS: We analyzed the cis expression quantitative trait locus for PRKCA marked by rs9912468 using 313 left ventricular specimens from European Ancestry patients...
March 2018: Circulation. Genomic and precision medicine
https://www.readbyqxmd.com/read/29538649/slit-robo-signalling-in-heart-development
#18
Juanjuan Zhao, Mathilda T M Mommersteeg
The Slit ligands and their Robo receptors are well-known for their roles during axon guidance in the central nervous system, but are still relatively unknown in the cardiac field. However, data from different animal models suggest a broad involvement of the pathway in many aspects of heart development, from cardiac cell migration and alignment, lumen formation, chamber formation, to the formation of the ventricular septum, semilunar and atrioventricular valves, caval veins and pericardium. Absence of one or more of the genes in the pathway results in defects ranging from bicuspid aortic valves to ventricular septal defects and abnormal venous connections to the heart...
March 10, 2018: Cardiovascular Research
https://www.readbyqxmd.com/read/29534099/genetic-analysis-of-zebrafish-homologs-of-human-foxq1-foxq1a-and-foxq1b-in-innate-immune-cell-development-and-bacterial-host-response
#19
Alison M Earley, Cameron T Dixon, Celia E Shiau
FOXQ1 is a member of the forkhead-box transcription factor family that has important functions in development, cancer, aging, and many cellular processes. The role of FOXQ1 in cancer biology has raised intense interest, yet much remains poorly understood. We investigated the possible function of the two zebrafish orthologs (foxq1a and foxq1b) of human FOXQ1 in innate immune cell development and function. We employed CRISPR-Cas9 targeted mutagenesis to create null mutations of foxq1a and foxq1b in zebrafish...
2018: PloS One
https://www.readbyqxmd.com/read/29526718/testis-transcriptome-alterations-in-zebrafish-danio-rerio-with-reduced-fertility-due-to-developmental-exposure-to-17%C3%AE-ethinyl-estradiol
#20
T Porseryd, N Reyhanian Caspillo, K Volkova, L Elabbas, T Källman, P Dinnétz, P-E Olsson, I Porsch-Hällström
17α-Ethinylestradiol (EE2 ) is a ubiquitous aquatic contaminant shown to decrease fish fertility at low concentrations, especially in fish exposed during development. The mechanisms of the decreased fertility are not fully understood. In this study, we perform transcriptome analysis by RNA sequencing of testes from zebrafish with previously reported lowered fertility due to exposure to low concentrations of EE2 during development. Fish were exposed to 1.2 and 1.6 ng/L (measured concentration; nominal concentrations 3 and 10 ng/L) of EE2 from fertilization to 80 days of age, followed by 82 days of remediation in clean water...
June 1, 2018: General and Comparative Endocrinology
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