keyword
MENU ▼
Read by QxMD icon Read
search

asparagine synthetase

keyword
https://www.readbyqxmd.com/read/29744685/outgrowth-of-rice-tillers-requires-availability-of-glutamine-in-the-basal-portions-of-shoots
#1
Miwa Ohashi, Keiki Ishiyama, Soichi Kojima, Noriyuki Konishi, Kazuhiro Sasaki, Mitsue Miyao, Toshihiko Hayakawa, Tomoyuki Yamaya
BACKGROUND: Our previous studies concluded that metabolic disorder in the basal portions of rice shoots caused by a lack of cytosolic glutamine synthetase1;2 (GS1;2) resulted in a severe reduction in the outgrowth of tillers. Rice mutants lacking GS1;2 (gs1;2 mutants) showed a remarkable reduction in the contents of both glutamine and asparagine in the basal portions of shoots. In the current study, we attempted to reveal the mechanisms for this decrease in asparagine content using rice mutants lacking either GS1;2 or asparagine synthetase 1 (AS1)...
May 9, 2018: Rice
https://www.readbyqxmd.com/read/29649183/transcriptome-and-metabolome-analyses-reveal-that-nitrate-strongly-promotes-nitrogen-and-carbon-metabolism-in-soybean-roots-but-tends-to-repress-it-in-nodules
#2
Shinji Ishikawa, Yuki Ono, Norikuni Ohtake, Kuni Sueyoshi, Sayuri Tanabata, Takuji Ohyama
Leguminous plants form root nodules with rhizobia that fix atmospheric dinitrogen (N₂) for the nitrogen (N) nutrient. Combined nitrogen sources, particular nitrate, severely repress nodule growth and nitrogen fixation activity in soybeans ( Glycine max [L.] Merr.). A microarray-based transcriptome analysis and the metabolome analysis were carried out for the roots and nodules of hydroponically grown soybean plants treated with 5 mM of nitrate for 24 h and compared with control without nitrate. Gene expression ratios of nitrate vs...
April 12, 2018: Plants (Basel, Switzerland)
https://www.readbyqxmd.com/read/29566166/the-nature-of-electron-acceptor-mniv-no3-triggers-differential-expression-of-genes-associated-with-stress-and-ammonium-limitation-responses-in-shewanella-algae-c6g3
#3
Axel Aigle, Patricia Bonin, Nicolas Fernandez -Nunez, Béatrice Loriod, Sophie Guasco, Aurélie Bergon, Fabrice Armougom, Chantal Iobbi-Nivol, Jean Imbert, Valérie Michotey
Shewanella algae C6G3 can reduce dissimilatively nitrate into ammonium and manganese-oxide (MnIV) into MnII. It has the unusual ability to produce anaerobically nitrite from ammonium in the presence of MnIV. To gain insight into their metabolic capabilities, global mRNA expression patterns were investigated by RNA-seq and qRT-PCR in cells growing with lactate and ammonium as carbon and nitrogen sources and with either MnIV or nitrate as electron acceptors. Gene exhibiting higher expression levels in the presence of MnIV belonged to functional categories of carbohydrate, coenzyme, lipid metabolisms and inorganic ion transport...
March 16, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29541827/biochemical-and-genetic-analyses-of-n-metabolism-in-maize-testcross-seedlings-2-roots
#4
Ignacio Trucillo Silva, Hari Kishan R Abbaraju, Lynne P Fallis, Hongjun Liu, Michael Lee, Kanwarpal S Dhugga
Intracellular factors differentially affected enzyme activities of N assimilation in the roots of maize testcrosses where alanine aminotransferase and glutamate synthase were the main enzymes regulating the levels of glutamate. N is a key macronutrient for plant growth and development. Breeding maize with improved efficiency in N use could help reduce environmental contamination as well as increase profitability for the farmers. Quantitative trait loci (QTL) mapping of traits related to N metabolism in the root tissue was undertaken in a maize testcross mapping population grown in hydroponic cultures...
March 14, 2018: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29518046/analysis-of-gene-regulatory-networks-of-maize-in-response-to-nitrogen
#5
Lu Jiang, Graham Ball, Charlie Hodgman, Anne Coules, Han Zhao, Chungui Lu
Nitrogen (N) fertilizer has a major influence on the yield and quality. Understanding and optimising the response of crop plants to nitrogen fertilizer usage is of central importance in enhancing food security and agricultural sustainability. In this study, the analysis of gene regulatory networks reveals multiple genes and biological processes in response to N. Two microarray studies have been used to infer components of the nitrogen-response network. Since they used different array technologies, a map linking the two probe sets to the maize B73 reference genome has been generated to allow comparison...
March 8, 2018: Genes
https://www.readbyqxmd.com/read/29414946/asparagine-bioavailability-governs-metastasis-in-a-model-of-breast-cancer
#6
Simon R V Knott, Elvin Wagenblast, Showkhin Khan, Sun Y Kim, Mar Soto, Michel Wagner, Marc-Olivier Turgeon, Lisa Fish, Nicolas Erard, Annika L Gable, Ashley R Maceli, Steffen Dickopf, Evangelia K Papachristou, Clive S D'Santos, Lisa A Carey, John E Wilkinson, J Chuck Harrell, Charles M Perou, Hani Goodarzi, George Poulogiannis, Gregory J Hannon
Using a functional model of breast cancer heterogeneity, we previously showed that clonal sub-populations proficient at generating circulating tumour cells were not all equally capable of forming metastases at secondary sites. A combination of differential expression and focused in vitro and in vivo RNA interference screens revealed candidate drivers of metastasis that discriminated metastatic clones. Among these, asparagine synthetase expression in a patient's primary tumour was most strongly correlated with later metastatic relapse...
February 15, 2018: Nature
https://www.readbyqxmd.com/read/29405484/report-of-four-novel-variants-in-asns-causing-asparagine-synthetase-deficiency-and-review-of-literature
#7
Chelna Galada, Malavika Hebbar, Leslie Lewis, Santosh Soans, Rajagopal Kadavigere, Anshika Srivastava, Stephanie Bielas, Katta M Girisha, Anju Shukla
No abstract text is available yet for this article.
February 6, 2018: Congenital Anomalies
https://www.readbyqxmd.com/read/29375865/clinical-whole-exome-sequencing-from-dried-blood-spot-identifies-novel-genetic-defect-underlying-asparagine-synthetase-deficiency
#8
Avinash Abhyankar, Michelle Lamendola-Essel, Kelly Brennan, Jessica L Giordano, Cecilia Esteves, Vanessa Felice, Ronald Wapner, Vaidehi Jobanputra
We add two novel variants to the existing mutation spectrum of ASNS gene. Loss of ASNS function should be suspected in newborns presenting with congenital microcephaly, intellectual disability, progressive cerebral atrophy, and intractable seizures. Acquisition and sequencing of stored newborn blood spot can be a valuable option when no biological samples are available from a deceased child.
January 2018: Clinical Case Reports
https://www.readbyqxmd.com/read/29316436/oncogenic-kras-regulates-amino-acid-homeostasis-and-asparagine-biosynthesis-via-atf4-and-alters-sensitivity-to-l-asparaginase
#9
Dana M Gwinn, Alex G Lee, Marcela Briones-Martin-Del-Campo, Crystal S Conn, David R Simpson, Anna I Scott, Anthony Le, Tina M Cowan, Davide Ruggero, E Alejandro Sweet-Cordero
KRAS is a regulator of the nutrient stress response in non-small-cell lung cancer (NSCLC). Induction of the ATF4 pathway during nutrient depletion requires AKT and NRF2 downstream of KRAS. The tumor suppressor KEAP1 strongly influences the outcome of activation of this pathway during nutrient stress; loss of KEAP1 in KRAS mutant cells leads to apoptosis. Through ATF4 regulation, KRAS alters amino acid uptake and asparagine biosynthesis. The ATF4 target asparagine synthetase (ASNS) contributes to apoptotic suppression, protein biosynthesis, and mTORC1 activation...
January 8, 2018: Cancer Cell
https://www.readbyqxmd.com/read/29299118/identification-of-a-cytogenetic-and-molecular-subgroup-of-acute-myeloid-leukemias-showing-sensitivity-to-l-asparaginase
#10
Salvatore Nicola Bertuccio, Salvatore Serravalle, Annalisa Astolfi, Annalisa Lonetti, Valentina Indio, Anna Leszl, Andrea Pession, Fraia Melchionda
L-Asparaginase (L-Asp) is an enzyme that catalyzes the hydrolysis of L-asparagine to L-aspartic acid, and its depletion induces leukemic cell death. L-Asp is an important component of treatment regimens for Acute Lymphoblastic Leukemia (ALL). Sensitivity to L-Asp is due to the absence of L-Asparagine synthetase (ASNS), the enzyme that catalyzes the biosynthesis of L-asparagine. ASNS gene is located on 7q21.3, and its increased expression in ALLs correlates with L-Asp resistance. Chromosome 7 monosomy (-7) is a recurrent aberration in myeloid disorders, particularly in adverse-risk Acute Myeloid Leukemias (AMLs) and therapy-related myeloid neoplasms (t-MN), that leads to a significant downregulation of the deleted genes, including ASNS ...
December 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/29279279/characterization-of-a-novel-variant-in-siblings-with-asparagine-synthetase-deficiency
#11
Stephanie J Sacharow, Elizabeth E Dudenhausen, Carrie L Lomelino, Lance Rodan, Christelle Moufawad El Achkar, Heather E Olson, Casie A Genetti, Pankaj B Agrawal, Robert McKenna, Michael S Kilberg
Asparagine Synthetase Deficiency (ASD) is a recently described inborn error of metabolism caused by bi-allelic pathogenic variants in the asparagine synthetase (ASNS) gene. ASD typically presents congenitally with microcephaly and severe, often medically refractory, epilepsy. Development is generally severely affected at birth. Tone is abnormal with axial hypotonia and progressive appendicular spasticity. Hyperekplexia has been reported. Neuroimaging typically demonstrates gyral simplification, abnormal myelination, and progressive cerebral atrophy...
March 2018: Molecular Genetics and Metabolism
https://www.readbyqxmd.com/read/29207147/effect-and-mechanism-of-rnai-targeting-wwtr1-on-biological-activity-of-gastric-cancer-cells-sgc7901
#12
Yuan Li, Fang-Liang Yang, Chun-Fu Zhu, Li-Ming Tang
Gastric cancer (GC) is one of the most common malignancies in the world. It is essential to develop novel targets and therapeutic approaches for GC, which requires identification of novel functional molecules. WW‑domain containing transcription regulator 1 (WWTR1) may activate many transcriptional factors and exhibit an important role in the development of various tissues in mammals. The results of the present study demonstrated that mRNA and protein levels of WWTR1 are increased in GC tissues and cell lines...
February 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29036367/phenylalanine-as-a-nitrogen-source-induces-root-growth-and-nitrogen-use-efficiency-in-populus-%C3%A3-canescens
#13
Yu Jiao, Yinghao Chen, Chaofeng Ma, Jingjing Qin, Thi Hong Nhung Nguyen, Di Liu, Honghao Gan, Shen Ding, Zhi-Bin Luo
To investigate the physiological responses of poplars to amino acids as sole nitrogen (N) sources, Populus × canescens (Ait.) Smith plants were supplied with one of three nitrogen fertilizers (NH4NO3, phenylalanine (Phe) or the mixture of NH4NO3 and Phe) in sand culture. A larger root system, and decreased leaf size and CO2 assimilation rate was observed in Phe- versus NH4NO3-treated poplars. Consistently, a greater root biomass and a decreased shoot growth were detected in Phe-supplied poplars. Decreased enzymatic activities of nitrate reductase (NR), glutamate synthase (GOGAT) and glutamate dehydrogenase (GDH) and elevated activities of nitrite reductase (NiR), phenylalanine ammonia lyase (PAL), glutamine synthetase (GS) and asparagine synthase (AS) were found in Phe-treated roots...
January 1, 2018: Tree Physiology
https://www.readbyqxmd.com/read/29025409/genes-for-asparagine-metabolism-in-lotus-japonicus-differential-expression-and-interconnection-with-photorespiration
#14
Margarita García-Calderón, Carmen M Pérez-Delgado, Alfredo Credali, José M Vega, Marco Betti, Antonio J Márquez
BACKGROUND: Asparagine is a very important nitrogen transport and storage compound in plants due to its high nitrogen/carbon ratio and stability. Asparagine intracellular concentration depends on a balance between asparagine biosynthesis and degradation. The main enzymes involved in asparagine metabolism are asparagine synthetase (ASN), asparaginase (NSE) and serine-glyoxylate aminotransferase (SGAT). The study of the genes encoding for these enzymes in the model legume Lotus japonicus is of particular interest since it has been proposed that asparagine is the principal molecule used to transport reduced nitrogen within the plant in most temperate legumes...
October 12, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28983307/qtl-mapping-by-whole-genome-re-sequencing-and-analysis-of-candidate-genes-for-nitrogen-use-efficiency-in-rice
#15
Xinghai Yang, Xiuzhong Xia, Zongqiong Zhang, Baoxuan Nong, Yu Zeng, Faqian Xiong, Yanyan Wu, Ju Gao, Guofu Deng, Danting Li
Nitrogen is a major nutritional element in rice production. However, excessive application of nitrogen fertilizer has caused severe environmental pollution. Therefore, development of rice varieties with improved nitrogen use efficiency (NUE) is urgent for sustainable agriculture. In this study, bulked segregant analysis (BSA) combined with whole genome re-sequencing (WGS) technology was applied to finely map quantitative trait loci (QTL) for NUE. A key QTL, designated as qNUE6 was identified on chromosome 6 and further validated by Insertion/Deletion (InDel) marker-based substitutional mapping in recombinants from F2 population (NIL-13B4 × GH998)...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28875262/erratum-to-asparagine-synthetase-deficiency-report-of-a-novel-mutation-and-review-of-literature
#16
Neerja Gupta, Vishal Vishnu Tewari, Manoj Kumar, Nitika Langeh, Aditi Gupta, Pallavi Mishra, Punit Kaur, Vedam Ramprasad, Sakthivel Murugan, Reema Kumar, Manisha Jana, Madhulika Kabra
No abstract text is available yet for this article.
December 2017: Metabolic Brain Disease
https://www.readbyqxmd.com/read/28842428/defective-lysosomal-clearance-of-autophagosomes-and-its-clinical-implications-in-nonalcoholic-steatohepatitis
#17
Xiaojuan Wang, Xiang Zhang, Eagle S H Chu, Xiaoting Chen, Wei Kang, Feng Wu, Ka-Fai To, Vincent W S Wong, Henry L Y Chan, Matthew T V Chan, Joseph J Y Sung, William K K Wu, Jun Yu
Autophagic impairment is implicated in nonalcoholic fatty liver disease (NAFLD), but the molecular mechanism is unclear. We found that autophagic flux was significantly inhibited in 3 murine models of NAFLD. Interestingly, the number of acidic organelles and the level of mature cathepsin D were reduced, suggesting defective lysosome acidification. Asparagine synthetase (ASNS) was induced by endoplasmic reticulum stress, leading to the generation of asparagine, which inhibited lysosome acidification. Both steatotic- and asparagine-treated hepatocytes showed reduced lysosomal acidity and retention of lysosomal calcium...
January 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28818378/nsr1-myr2-is-a-negative-regulator-of-asn1-expression-and-its-possible-involvement-in-regulation-of-nitrogen-reutilization-in-arabidopsis
#18
Yoshimi Nakano, Yuki Naito, Toshitsugu Nakano, Namie Ohtsuki, Kaoru Suzuki
Nitrogen (N) is a major macronutrient that is essential for plant growth. It is important for us to understand the key genes that are involved in the regulation of N utilization. In this study, we focused on a GARP-type transcription factor known as NSR1/MYR2, which has been reported to be induced under N-deficient conditions. Our results demonstrated that NSR1/MYR2 has a transcriptional repression activity and is specifically expressed in vascular tissues, especially in phloem throughout the plant under daily light-dark cycle regulation...
October 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28818367/metabolic-features-involved-in-drought-stress-tolerance-mechanisms-in-peanut-nodules-and-their-contribution-to-biological-nitrogen-fixation
#19
Ana Laura Furlan, Eliana Bianucci, Stella Castro, Karl-Josef Dietz
Legumes belong to the most important crops worldwide. They increase soil fertility due their ability to establish symbiotic associations with soil microorganisms, known as rhizobia, capable of fixing nitrogen from the atmosphere. However, they are frequently exposed to abiotic stress conditions in particular drought. Such adverse conditions impair the biological nitrogen fixation (BNF) and depend largely on the legume. Therefore, two peanut cultivars with contrasting tolerance to drought, namely the more tolerant EC-98 and the sensitive Granoleico, were investigated to elucidate the relative contribution of BNF to the tolerance to drought...
October 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28811348/a-critical-role-of-glutamine-and-asparagine-%C3%AE-nitrogen-in-nucleotide-biosynthesis-in-cancer-cells-hijacked-by-an-oncogenic-virus
#20
Ying Zhu, Tingting Li, Suzane Ramos da Silva, Jae-Jin Lee, Chun Lu, Hyungjin Eoh, Jae U Jung, Shou-Jiang Gao
While glutamine is a nonessential amino acid that can be synthesized from glucose, some cancer cells primarily depend on glutamine for their growth, proliferation, and survival. Numerous types of cancer also depend on asparagine for cell proliferation. The underlying mechanisms of the glutamine and asparagine requirement in cancer cells in different contexts remain unclear. In this study, we show that the oncogenic virus Kaposi's sarcoma-associated herpesvirus (KSHV) accelerates the glutamine metabolism of glucose-independent proliferation of cancer cells by upregulating the expression of numerous critical enzymes, including glutaminase 2 (GLS2), glutamate dehydrogenase 1 (GLUD1), and glutamic-oxaloacetic transaminase 2 (GOT2), to support cell proliferation...
August 15, 2017: MBio
keyword
keyword
75897
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"