keyword
MENU ▼
Read by QxMD icon Read
search

MvaT

keyword
https://www.readbyqxmd.com/read/26833155/amino-acid-substitutions-account-for-most-mexs-alterations-in-clinical-nfxc-mutants-of-pseudomonas-aeruginosa
#1
Charlotte Richardot, Paulo Juarez, Katy Jeannot, Isabelle Patry, Patrick Plésiat, Catherine Llanes
Multidrug-resistant mutants ofPseudomonas aeruginosathat overproduce the active efflux system MexEF-OprN (callednfxCmutants) have rarely been characterized in the hospital setting. Screening of 221 clinical strains exhibiting a reduced susceptibility to ciprofloxacin (a substrate of MexEF-OprN) and imipenem (a substrate of the negatively coregulated porin OprD) led to the identification of 43 (19.5%)nfxCmutants. Subsequent analysis of 22 nonredundant mutants showed that, in contrast to theirin vitro-selected counterparts, only 3 of them (13...
April 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/26594207/antibacterial-phage-orfans-of-pseudomonas-aeruginosa-phage-luz24-reveal-a-novel-mvat-inhibiting-protein
#2
Jeroen Wagemans, Anne-Sophie Delattre, Birgit Uytterhoeven, Jeroen De Smet, William Cenens, Abram Aertsen, Pieter-Jan Ceyssens, Rob Lavigne
The functional elucidation of small unknown phage proteins ('ORFans') presents itself as one of the major challenges of bacteriophage molecular biology. In this work, we mined the Pseudomonas aeruginosa-infecting phage LUZ24 proteome for antibacterial and antibiofilm proteins against its host. Subsequently, their putative host target was identified. In one example, we observed an interaction between LUZ24 gp4 and the host transcriptional regulator MvaT. The polymerization of MvaT across AT-rich DNA strands permits gene silencing of foreign DNA, thereby limiting any potentially adverse effects of such DNA...
2015: Frontiers in Microbiology
https://www.readbyqxmd.com/read/26247404/distribution-of-putative-xenogeneic-silencers-in-prokaryote-genomes
#3
Ernesto Perez-Rueda, J Antonio Ibarra
Gene silencing is an important function as it keeps newly acquired foreign DNA repressed, thereby avoiding possible deleterious effects in the host organism. Known transcriptional regulators associated with this process are called xenogeneic silencers (XS) and belong to either the H-NS, Lsr2, MvaT or Rok families. In the work described here we looked for XS-like regulators and their distribution in prokaryotic organisms was evaluated. Our analysis showed that putative XS regulators similar to H-NS, Lsr2, MvaT or Rok are present only in bacteria (31...
October 2015: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/26068099/a-novel-at-rich-dna-recognition-mechanism-for-bacterial-xenogeneic-silencer-mvat
#4
Pengfei Ding, Kirsty A McFarland, Shujuan Jin, Grace Tong, Bo Duan, Ally Yang, Timothy R Hughes, Jun Liu, Simon L Dove, William Wiley Navarre, Bin Xia
Bacterial xenogeneic silencing proteins selectively bind to and silence expression from many AT rich regions of the chromosome. They serve as master regulators of horizontally acquired DNA, including a large number of virulence genes. To date, three distinct families of xenogeneic silencers have been identified: H-NS of Proteobacteria, Lsr2 of the Actinomycetes, and MvaT of Pseudomonas sp. Although H-NS and Lsr2 family proteins are structurally different, they all recognize the AT-rich DNA minor groove through a common AT-hook-like motif, which is absent in the MvaT family...
June 2015: PLoS Pathogens
https://www.readbyqxmd.com/read/25952329/nucleoid-associated-proteins-encoded-on-plasmids-occurrence-and-mode-of-function
#5
Masaki Shintani, Chiho Suzuki-Minakuchi, Hideaki Nojiri
Nucleoid-associated proteins (NAPs) play a role in changing the shape of microbial DNA, making it more compact and affecting the regulation of transcriptional networks in host cells. Genes that encode NAPs include H-NS family proteins (H-NS, Ler, MvaT, BpH3, Bv3F, HvrA, and Lsr2), FIS, HU, IHF, Lrp, and NdpA, and are found in both microbial chromosomes and plasmid DNA. In the present study, NAP genes were distributed among 442 plasmids out of 4602 plasmid sequences, and many H-NS family proteins, and HU, IHF, Lrp, and NdpA were found in plasmids of Alpha-, Beta-, and Gammaproteobacteria, while HvrA, Lsr2, HU, and Lrp were found in other classes including Actinobacteria and Bacilli...
July 2015: Plasmid
https://www.readbyqxmd.com/read/25845844/cysb-negatively-affects-the-transcription-of-pqsr-and-pseudomonas-quinolone-signal-production-in-pseudomonas-aeruginosa
#6
John M Farrow, L Lynn Hudson, Greg Wells, James P Coleman, Everett C Pesci
UNLABELLED: Pseudomonas aeruginosa is a Gram-negative bacterium that is ubiquitous in the environment, and it is an opportunistic pathogen that can infect a variety of hosts, including humans. During the process of infection, P. aeruginosa coordinates the expression of numerous virulence factors through the production of multiple cell-to-cell signaling molecules. The production of these signaling molecules is linked through a regulatory network, with the signal N-(3-oxododecanoyl) homoserine lactone and its receptor LasR controlling the induction of a second acyl-homoserine lactone signal and the Pseudomonas quinolone signal (PQS)...
June 15, 2015: Journal of Bacteriology
https://www.readbyqxmd.com/read/25372370/single-molecule-study-on-histone-like-nucleoid-structuring-protein-h-ns-paralogue-in-pseudomonas-aeruginosa-mvau-bears-dna-organization-mode-similarities-to-mvat
#7
Ricksen S Winardhi, Sandra Castang, Simon L Dove, Jie Yan
Pseudomonas aeruginosa contains two distinct members of H-NS family of nucleoid-structuring proteins: MvaT and MvaU. Together, these proteins bind to the same regions of the chromosome and function coordinately in the regulation of hundreds of genes. Due to their structural similarity, they can associate to form heteromeric complexes. These findings left us wondering whether they bear similar DNA binding properties that underlie their gene-silencing functions. Using single-molecule stretching and imaging experiments, we found striking similarities in the DNA organization modes of MvaU compared to the previously studied MvaT...
2014: PloS One
https://www.readbyqxmd.com/read/25137042/oligomerization-mechanisms-of-an-h-ns-family-protein-pmr-encoded-on-the-plasmid-pcar1-provide-a-molecular-basis-for-functions-of-h-ns-family-members
#8
Chiho Suzuki, Kohei Kawazuma, Shoichiro Horita, Tohru Terada, Masaru Tanokura, Kazunori Okada, Hisakazu Yamane, Hideaki Nojiri
Enterobacterial H-NS-like proteins and Pseudomonas MvaT-like proteins share low homology at the amino acid sequence level, but both can function as xenogeneic silencers and are included in the H-NS family of proteins. H-NS family members have dimerization/oligomerization and DNA-binding domains connected by a flexible linker and form large nucleoprotein complexes using both domains. Pmr, an MvaT-like protein encoded on the IncP-7 carbazole-degradative plasmid pCAR1, is a key regulator of an interaction between pCAR1 and its host Pseudomonas putida KT2440...
2014: PloS One
https://www.readbyqxmd.com/read/25006001/robustness-of-the-near-infrared-spectroscopy-method-determined-using-univariate-and-multivariate-approach
#9
Milica Pojić, Jasna Mastilović, Nineta Majcen
The robustness assessment is a part of a method validation protocol, during which several characteristics of an analytical method are also evaluated (e.g. accuracy, repeatability, reproducibility, linearity, intermediate precision, measurement uncertainty) in order to assess its fitness for purpose. The purpose of robustness assessment of the near infrared spectroscopy method (NIRS) is to indicate which factor significantly influence the obtained results, as well as to point to the potential problems that might occur in the routine application of the method...
October 1, 2012: Food Chemistry
https://www.readbyqxmd.com/read/24497189/bswr-controls-bacterial-motility-and-biofilm-formation-in-pseudomonas-aeruginosa-through-modulation-of-the-small-rna-rsmz
#10
Chao Wang, Fuzhou Ye, Veerendra Kumar, Yong-Gui Gao, Lian-Hui Zhang
Pseudomonas aeruginosa relies on cell motility and ability to form biofilms to establish infections; however, the mechanism of regulation remains obscure. Here we report that BswR, a xenobiotic response element-type transcriptional regulator, plays a critical role in regulation of bacterial motility and biofilm formation in P. aeruginosa. Transcriptomic and biochemical analyses showed that BswR counteracts the repressor activity of MvaT, controls the transcription of small RNA rsmZ and regulates the biogenesis of bacterial flagella...
April 2014: Nucleic Acids Research
https://www.readbyqxmd.com/read/24436158/serum-influences-the-expression-of-pseudomonas-aeruginosa-quorum-sensing-genes-and-qs-controlled-virulence-genes-during-early-and-late-stages-of-growth
#11
COMPARATIVE STUDY
Cassandra Kruczek, Uzma Qaisar, Jane A Colmer-Hamood, Abdul N Hamood
In response to diverse environmental stimuli at different infection sites, Pseudomonas aeruginosa, a serious nosocomial pathogen, coordinates the production of different virulence factors through a complicated network of the hierarchical quorum-sensing (QS) systems including the las, rhl, and the 2-alkyl-4-quinolone-related QS systems. We recently showed that at early stages of growth serum alters the expression of numerous P. aeruginosa genes. In this study, we utilized transcriptional analysis and enzyme assays to examine the effect of serum on the QS and QS-controlled virulence factors during early and late phases of growth of the P...
February 2014: MicrobiologyOpen
https://www.readbyqxmd.com/read/22934351/-mvat-and-mvav-transcriptionally-regulate-pcoi-pcor-quorum-sensing-system-in-pseudomonas-fluorescens-2p24
#12
Xiaogang Wu, Yarui Wei, Jiucheng Liu, Liqun Zhang
OBJECTIVE: Pseudomonas fluorescens 2P24 is an effective biocontrol agent for soil-borne plant diseases caused by microbial pathogens. The PcoI/PcoR quorum-sensing system, which influences the colonization ability of 2P24 on wheat rhizosphere, is an important factor for disease suppression. In this study we performed random mutagenesis to screen novel regulators of the pcoI gene, a biosynthase gene responsible for N-acyl-homoserine lactone (AHL) production. METHODS: A gacA gene mutant carrying a pcoI-lacZ fusion was employed as the reporter strain and subjected to a random mini-Tn5 insertion mutagenesis...
June 4, 2012: Wei Sheng Wu Xue Bao, Acta Microbiologica Sinica
https://www.readbyqxmd.com/read/22821971/basis-for-the-essentiality-of-h-ns-family-members-in-pseudomonas-aeruginosa
#13
Sandra Castang, Simon L Dove
Members of the histone-like nucleoid-structuring (H-NS) family of proteins have been shown to play important roles in silencing gene expression and in nucleoid compaction. In Pseudomonas aeruginosa, the two H-NS family members MvaT and MvaU are thought to bind the same AT-rich regions of the chromosome and function coordinately to control a common set of genes. Here we present evidence that the loss of both MvaT and MvaU cannot be tolerated because it results in the production of Pf4 phage that superinfect and kill cells or inhibit their growth...
September 2012: Journal of Bacteriology
https://www.readbyqxmd.com/read/22798496/higher-order-oligomerization-is-required-for-h-ns-family-member-mvat-to-form-gene-silencing-nucleoprotein-filament
#14
Ricksen S Winardhi, Wenbo Fu, Sandra Castang, Yanan Li, Simon L Dove, Jie Yan
MvaT from Pseudomonas aeruginosa is a member of the histone-like nucleoid structuring protein (H-NS) family of nucleoid-associated proteins widely spread among Gram-negative bacteria that functions to repress the expression of many genes. Recently, it was reported that H-NS from Escherichia coli can form rigid nucleoproteins filaments on DNA, which are important for their gene-silencing function. This raises a question whether the gene-silencing function of MvaT, which has only ∼18% sequence similarity to H-NS, is also based on the formation of nucleoprotein filaments...
October 2012: Nucleic Acids Research
https://www.readbyqxmd.com/read/22265250/silencing-of-foreign-dna-in-bacteria
#15
REVIEW
Sabrina S Ali, Bin Xia, Jun Liu, William Wiley Navarre
Xenogeneic silencing proteins facilitate horizontal gene transfer by silencing expression of AT-rich sequences. By virtue of their activity these proteins serve as master regulators of a variety of important functions including motility, drug resistance, and virulence. Three families of silencers have been identified to date: the H-NS like proteins of Gram-negative bacteria, the MvaT like proteins of Pseudomonacae, and the Lsr2 proteins of Actinobacteria. Structural and biochemical characterization of these proteins have revealed that they share surprising commonalities in mechanism and function despite extensive divergence in both sequence and structure...
April 2012: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/21911574/role-of-the-mexef-oprn-efflux-system-in-low-level-resistance-of-pseudomonas-aeruginosa-to-ciprofloxacin
#16
Catherine Llanes, Thilo Köhler, Isabelle Patry, Barbara Dehecq, Christian van Delden, Patrick Plésiat
In this study, we investigated the resistance mechanisms to fluoroquinolones of 85 non-cystic fibrosis strains of Pseudomonas aeruginosa exhibiting a reduced susceptibility to ciprofloxacin (MICs from 0.25 to 2 μg/ml). In addition to MexAB-OprM (31 of 85 isolates) and MexXY/OprM (39 of 85), the MexEF-OprN efflux pump (10 of 85) was found to be commonly upregulated in this population that is considered susceptible or of intermediate susceptibility to ciprofloxacin, according to current breakpoints. Analysis of the 10 MexEF-OprN overproducers (nfxC mutants) revealed the presence of various mutations in the mexT (2 isolates), mexS (5 isolates), and/or mvaT (2 isolates) genes, the inactivation of which is known to increase the expression of the mexEF-oprN operon in reference strain PAO1-UW...
December 2011: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/21350637/distribution-of-genes-encoding-nucleoid-associated-protein-homologs-in-plasmids
#17
Toshiharu Takeda, Choong-Soo Yun, Masaki Shintani, Hisakazu Yamane, Hideaki Nojiri
Bacterial nucleoid-associated proteins (NAPs) form nucleoprotein complexes and influence the expression of genes. Recent studies have shown that some plasmids carry genes encoding NAP homologs, which play important roles in transcriptional regulation networks between plasmids and host chromosomes. In this study, we determined the distributions of the well-known NAPs Fis, H-NS, HU, IHF, and Lrp and the newly found NAPs MvaT and NdpA among the whole-sequenced 1382 plasmids found in Gram-negative bacteria. Comparisons between NAP distributions and plasmid features (size, G+C content, and putative transferability) were also performed...
2011: International Journal of Evolutionary Biology
https://www.readbyqxmd.com/read/20815825/high-order-oligomerization-is-required-for-the-function-of-the-h-ns-family-member-mvat-in-pseudomonas-aeruginosa
#18
Sandra Castang, Simon L Dove
H-NS is an abundant DNA-binding protein that has been implicated in the silencing of foreign DNA in several different bacteria. The ability of H-NS dimers to form higher-order oligomers is thought to aid the polymerization of the protein across AT-rich stretches of DNA and facilitate gene silencing. Although the oligomerization of H-NS from enteric bacteria has been the subject of intense investigation, little is known regarding the oligomerization of H-NS family members from bacteria outside of the enterobacteriaceae, many of which share little sequence similarity with their enteric counterparts...
November 2010: Molecular Microbiology
https://www.readbyqxmd.com/read/19832907/pseudomonas-aeruginosa-pa14-cupd-transcription-is-activated-by-the-rcsb-response-regulator-but-repressed-by-its-putative-cognate-sensor-rcsc
#19
Gianlucca G Nicastro, Ana Laura Boechat, Cecília M Abe, Gilberto H Kaihami, Regina L Baldini
The opportunistic pathogen Pseudomonas aeruginosa PA14 possesses four fimbrial cup clusters, which may confer the ability to adapt to different environments. cupD lies in the pathogenicity island PAPI-1 next to genes coding for a putative phosphorelay system composed of the hybrid histidine kinase RcsC and the response regulator RcsB. The main focus of this work was the regulation of cupD at the mRNA level. It was found that the HN-S-like protein MvaT does not exert a strong influence on cupD transcript levels, as it does for cupA...
November 2009: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/19788653/identification-of-genes-regulated-by-the-mvat-like-paralogues-tura-and-turb-of-pseudomonas-putida-kt2440
#20
Francesco Renzi, Emanuela Rescalli, Enrica Galli, Giovanni Bertoni
The genome of the Pseudomonas putida strain KT2440 contains five paralogous proteins (TurA, TurB, TurC, TurD and TurE) of the H-NS-like MvaT class of transcription regulators. TurA and TurB belong to groups I and II, respectively, both containing orthologous MvaT proteins that are present in all Pseudomonadaceae species. On the contrary, TurC, TurD and TurE belong to group III, which contains species-specific paralogous MvaT proteins. We analysed the global effects on the P. putida KT2440 transcriptome of eliminating the conserved TurA and TurB proteins, which had been identified in our previous studies aimed to search for novel specific co-regulators of the upper TOL operon for toluene biodegradation...
January 2010: Environmental Microbiology
keyword
keyword
7585
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"