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https://www.readbyqxmd.com/read/27914529/agglutinin-isolated-from-arisema-heterophyllum-blume-induces-apoptosis-and-autophagy-in-a549-cells-through-inhibiting-pi3k-akt-pathway-and-inducing-er-stress
#1
Li-Xing Feng, Peng Sun, Tian Mi, Miao Liu, Wang Liu, Si Yao, Yi-Min Cao, Xiao-Lu Yu, Wan-Ying Wu, Bao-Hong Jiang, Min Yang, De-An Guo, Xuan Liu
Arisaema heterophyllum Blume is one of the three medicinal plants known as traditional Chinese medicine Rhizoma Arisaematis (RA). RA has been popularly used to treat patients with convulsions, inflammation, and cancer for a long time. However, the underlying mechanisms for RA effects are still unclear. The present study was designed to determine the cytotoxicity of agglutinin isolated from Arisema heterophyllum Blume (AHA) and explore the possible mechanisms in human non-small-cell lung cancer A549 cells. AHA with purity up to 95% was isolated and purified from Arisaema heterophyllum Blume using hydrophobic interaction chromatography...
November 2016: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/27900564/effects-of-rnai-mediated-tusc3-silencing-on-radiation-induced-autophagy-and-radiation-sensitivity-of-human-lung-adenocarcinoma-cell-line-a549-under-hypoxic-condition
#2
Ya-Guang Li, Nai-Xin Liang, Ying-Zhi Qin, Dong-Jie Ma, Chang-Jin Huang, Lei Liu, Shan-Qing Li
This study examined the effects of RNAi-mediated TUSC3 silencing on radiation-induced autophagy and radiation sensitivity of human lung adenocarcinoma cell line A549 under hypoxic condition. Different CoCl2 concentrations were used to treat A549 cells and establish a CoCl2-induced hypoxic model of A549 cells. MTT and clone formation assays were used to determine the effects of different concentrations of CoCl2 on the growth and proliferation of A549 cells treated by different doses of X-ray irradiation. The siRNA-expressing vector was transfected by liposomes and for silencing of TUSC3...
November 30, 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/27899819/4-cholesten-3-one-suppresses-lung-adenocarcinoma-metastasis-by-regulating-translocation-of-hmgb1-hif1%C3%AE-and-caveolin-1
#3
Jinben Ma, Guobin Fu, Jing Wu, Shaoxian Han, Lishan Zhang, Ming Yang, Yong Yu, Mengyuan Zhang, Yanliang Lin, Yibing Wang
Metastasis is a great challenge in lung adenocarcinoma (ADC) therapy. Cholesterol has been implicated in ADC metastasis. 4-cholesten-3-one, as cholesterol metabolite and analog, can substitute membrane cholesterol and increase membrane fluidity. In this study, we explored the possibility that 4-cholesten-3-one inhibited ADC metastasis. Low-dose 4-cholesten-3-one significantly restrained ADC cells migration and invasion with little effects on cells viabilities. Further investigation showed that 4-cholesten-3-one promoted ROS generation, which transiently activated AMPKα1, increased HIF1α expression, reduced Bcl-2 expression and caused autophagy...
September 22, 2016: Cell Death & Disease
https://www.readbyqxmd.com/read/27890727/synthetic-tambjamine-analogues-induce-mitochondrial-swelling-and-lysosomal-dysfunction-leading-to-autophagy-blockade-and-necrotic-cell-death-in-lung-cancer
#4
Ananda M Rodilla, Luís Korrodi-Gregório, Elsa Hernando, Pilar Manuel-Manresa, Roberto Quesada, Ricardo Pérez-Tomás, Vanessa Soto-Cerrato
Current pharmacological treatments for lung cancer show very poor clinical outcomes, therefore, the development of novel anticancer agents with innovative mechanisms of action is urgently needed. Cancer cells have a reversed pH gradient compared to normal cells, which favours cancer progression by promoting proliferation, metabolic adaptation and evasion of apoptosis. In this regard, the use of ionophores to modulate intracellular pH appears as a promising new therapeutic strategy. Indeed, there is a growing body of evidence supporting ionophores as novel antitumour drugs...
November 24, 2016: Biochemical Pharmacology
https://www.readbyqxmd.com/read/27888631/autophagy-decreases-alveolar-macrophage-apoptosis-by-attenuating-endoplasmic-reticulum-stress-and-oxidative-stress
#5
Tao Fan, Lei Chen, Zhixin Huang, Zhangfan Mao, Wei Wang, Boyou Zhang, Yao Xu, Shize Pan, Hao Hu, Qing Geng
To study the impact of autophagy on alveolar macrophage apoptosis and its mechanism in the early stages of hypoxia, we established a cell hypoxia-reoxygenation model and orthotopic left lung ischemia-reperfusion model. Rat alveolar macrophages stably expressing RFP-LC3 were treated with autophagy inhibitor (3-methyladenine, 3-MA) or autophagy promoter (rapamycin), followed by hypoxia-reoxygenation treatment 2 h, 4 h or 6 h later. Twenty Sprague-Dawley male rats were randomly divided into four different groups: no blocking of left lung hilum (model group), left lung hilum blocked for 1h with DMSO lavage (control group), left lung hilum blocked for 1 h with 100 ml/kg 3-MA (5 μmol/L) lavage (3-MA group), and left lung hilum blocked for 1 h with 100 ml/kg rapamycin (250 nmol/L) lavage (rapamycin group)...
November 24, 2016: Oncotarget
https://www.readbyqxmd.com/read/27886851/the-role-of-necroptosis-in-pulmonary-diseases
#6
REVIEW
Kenji Mizumura, Shuichiro Maruoka, Yasuhiro Gon, Augustine M K Choi, Shu Hashimoto
By regulating the cell number and eliminating harmful cells, programmed cell death plays a critical role in development, homeostasis, and disease. While apoptosis is a recognized form of programmed cell death, necrosis was considered a type of uncontrolled cell death induced by extreme physical or chemical stress. However, recent studies have revealed the existence of a genetically programmed and regulated form of necrosis, termed necroptosis. Necroptosis is defined as necrotic cell death that is dependent on receptor-interacting protein kinase 3 (RIPK3)...
November 2016: Respiratory Investigation
https://www.readbyqxmd.com/read/27886850/cellular-senescence-and-autophagy-in-the-pathogenesis-of-chronic-obstructive-pulmonary-disease-copd-and-idiopathic-pulmonary-fibrosis-ipf
#7
REVIEW
Kazuyoshi Kuwano, Jun Araya, Hiromichi Hara, Shunsuke Minagawa, Naoki Takasaka, Saburo Ito, Kenji Kobayashi, Katsutoshi Nakayama
Aging is associated with impairments in homeostasis. Although aging and senescence are not equivalent, the number of senescent cells increases with aging. Cellular senescence plays important roles in tissue repair or remodeling, as well as embryonic development. Autophagy is a process of lysosomal self-degradation that maintains a homeostatic balance between the synthesis, degradation, and recycling of cellular proteins. Autophagy diminishes with aging; additionally, accelerated aging can be attributed to reduced autophagy...
November 2016: Respiratory Investigation
https://www.readbyqxmd.com/read/27871936/commd9-promotes-tfdp1-e2f1-transcriptional-activity-via-interaction-with-tfdp1-in-non-small-cell-lung-cancer
#8
Weihua Zhan, Wenjuan Wang, Tianyu Han, Caifeng Xie, Tingting Zhang, Mingxi Gan, Jian-Bin Wang
COMMD protein family is an evolutionarily conserved gene family implicated in a number of critical processes including inflammation, copper homeostasis, sodium balance, endosomal sorting and cancer. In an effort to profile the expression pattern of COMMD family in several non-small cell lung cancer (NSCLC) cell lines, we found that compared with that in human bronchial epithelial (HBE) cells, the mRNA expression levels of five COMMD genes including COMMD3, COMMD4, COMMD5, COMMD6 and COMMD8 were significantly down-regulated, whereas COMMD9 was up-regulated in NSCLC cell lines...
November 19, 2016: Cellular Signalling
https://www.readbyqxmd.com/read/27846374/ubiquitin-coated-nanodiamonds-bind-to-autophagy-receptors-for-entry-into-the-selective-autophagy-pathway
#9
Kuang-Kai Liu, Wei-Ru Qiu, Emmanuel Naveen Raj, Huei-Fang Liu, Hou-Syun Huang, Yu-Wei Lin, Chien-Jen Chang, Ting-Hua Chen, Chinpiao Chen, Huan-Cheng Chang, Jenn-Kang Hwang, Jui-I Chao
Selective macroautophagy/autophagy plays a pivotal role in the processing of foreign pathogens and cellular components to maintain homeostasis in human cells. To date, numerous studies have demonstrated the uptake of nanoparticles by cells, but their intracellular processing through selective autophagy remains unclear. Here we show that carbon-based nanodiamonds (NDs) coated with ubiquitin (Ub) bind to autophagy receptors (SQSTM1 [sequestosome 1], OPTN [optineurin], and CALCOCO2/NDP52 [calcium binding and coiled-coil domain 2]) and are then linked to MAP1LC3/LC3 (microtubule associated protein 1 light chain 3) for entry into the selective autophagy pathway...
November 15, 2016: Autophagy
https://www.readbyqxmd.com/read/27836757/pm2-5-exposure-induced-autophagy-is-mediated-by-lncrna-loc146880-which-also-promotes-the-migration-and-invasion-of-lung-cancer-cells
#10
Xiaobei Deng, Nannan Feng, Min Zheng, Xiaofei Ye, Hongyan Lin, Xiao Yu, Zhihua Gan, Zheng Fang, Huan Zhang, Ming Gao, Zhi-Jie Zheng, Herbert Yu, Wenjun Ding, Biyun Qian
BACKGROUND: Evidence shows that individuals who are under long-term exposure to environmental PM2.5 are at increased risk of lung cancer. Various laboratory experiments also suggest several mechanistic links between PM2.5 exposure and lung carcinogenesis. However, a long non-coding RNA (lncRNA) mediated pathogenic change after PM2.5 exposure and its potential roles in tumorigenesis and disease progression have not been reported. METHODS: Cytotoxicity induced by PM2...
November 9, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27835930/master-autophagy-regulator-transcription-factor-eb-tfeb-regulates-cigarette-smoke-induced-autophagy-impairment-and-copd-emphysema-pathogenesis
#11
Manish Bodas, Neel Patel, David Silverberg, Kyla Walworth, Neeraj Vij
AIMS: Recent studies have shown that cigarette smoke (CS)-induced oxidative-stress impairs autophagy resulting in aggresome-formation that correlates with the severity of COPD-emphysema. Although, specific step in autophagy-pathway that is impaired is unknown. Hence, in this study we aimed to evaluate the role of master autophagy-regulator and transcription factor-EB (TFEB) in CS-induced COPD-emphysema pathogenesis. RESULTS: We first observed that TFEB-accumulates in the peri-nuclear spaces of the COPD lung tissues of tobacco-smokers and severe-emphysema subjects...
November 11, 2016: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/27823620/immunomodulatory-proteins-fip-gts-and-chloroquine-induce-caspase-independent-cell-death-via-autophagy-for-resensitizing-cisplatin-resistant-urothelial-cancer-cells
#12
I-Lun Hsin, Shao-Chuan Wang, Jian-Ri Li, Tsai-Chun Ciou, Chih-Hsien Wu, Hung-Ming Wu, Jiunn-Liang Ko
BACKGROUND: Chloroquine, a lysosomal inhibitor, is used for malaria, rheumatoid arthritis, and lupus erythematosus therapy. In our previous study, FIP-gts, an immunomodulatory protein from Ganoderma tsugae, inhibited cell viability in lung cancer cells and urothelial cancer cells. Urothelial carcinoma is the most common type of bladder cancer. Cisplatin resistance is an important issue in urothelial carcinoma therapy. PURPOSE: The aim of this study is to investigate the effect of combination treatment with FIP-gts and chloroquine on cytotoxicity to resensitize the cisplatin-resistant cells...
December 1, 2016: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/27822066/inhibitory-effects-of-silibinin-on-proliferation-and-lung-metastasis-of-human-high-metastasis-cell-line-of-salivary-gland-adenoid-cystic-carcinoma-via-autophagy-induction
#13
Canhua Jiang, Shufang Jin, Zhisheng Jiang, Jie Wang
OBJECTIVE: To investigate the possible mechanisms and effects of silibinin (SIL) on the proliferation and lung metastasis of human lung high metastasis cell line of salivary gland adenoid cystic carcinoma (ACC-M). METHODS: A methyl thiazolyl tetrazolium assay was performed to detect the inhibitory effects of SIL on the proliferation of ACC-M cells in vitro. Fluorescence microscopy and transmission electron microscopy were used to observe the autophagic process. Western blot was performed to detect the expression of microtube-related protein 1 light-chain 3 (LC3)...
2016: OncoTargets and Therapy
https://www.readbyqxmd.com/read/27814589/er-chaperone-grp78-regulates-autophagy-by-modulation-of-p53-localization
#14
Mohd Kamil, Ejazul Haque, Safia Irfan, Saba Sheikh, Adria Hasan, Aamir Nazir, Mohtashim Lohani, Snober S Mir
GRP78 (glucose regulated protein 78) is a major Endoplasmic Reticulum (ER) chaperone that plays a pivotal role in normal ER functioning. Its increased expression also works as an indicator of ER stress. Its anti-apoptotic and pro-autophagic activity makes it an intriguing target to study the relationship between GRP78 and p53, which is also a major regulator of apoptosis and autophagy. Here, we studied the effect of Rotenone and Parathion on human lung cancer cells (A549 cell line) specifically with respect to ER stress and its association with different cell death pathways...
January 1, 2017: Frontiers in Bioscience (Elite Edition)
https://www.readbyqxmd.com/read/27811372/the-induction-of-autophagy-against-mitochondria-mediated-apoptosis-in-lung-cancer-cells-by-a-ruthenium-ii-imidazole-complex
#15
Lanmei Chen, Guodong Li, Fa Peng, Xinming Jie, Guangzhi Dongye, Kangrong Cai, Ruibing Feng, Baojun Li, Qingwang Zeng, Kaiyi Lun, Jincan Chen, Bilian Xu
In the present study, it was found that the ruthenium (II) imidazole complex [Ru(Im)4(dppz)]2+ (Ru1) could induce significant growth inhibition and apoptosis in A549 and NCI-H460 cells. Apart from the induction of apoptosis, it was reported for the first time that Ru1 induced an autophagic response in A549 and NCI-H460 cells as evidenced by the formation of autophagosomes, acidic vesicular organelles (AVOs), and the up-regulation of LC3-II. Furthermore, scavenging of reactive oxygen species (ROS) by antioxidant NAC or Tiron inhibited the release of cytochrome c, caspase-3 activity, and eventually rescued cancer cells from Ru1-mediated apoptosis, suggesting that Ru1 inducing apoptosis was partially caspase 3-dependent by triggering ROS-mediated mitochondrial dysfunction in A549 and NCI-H460 cells...
November 2, 2016: Oncotarget
https://www.readbyqxmd.com/read/27793929/inhibition-of-glutaminolysis-inhibits-cell-growth-via-down-regulating-mtorc1-signaling-in-lung-squamous-cell-carcinoma
#16
Xulu Ye, Qiliang Zhou, Yoshifumi Matsumoto, Masato Moriyama, Shun Kageyama, Masaaki Komatsu, Seijiro Satoh, Masanori Tsuchida, Yasuo Saijo
BACKGROUND/AIM: Inhibition of glutaminolysis has been reported as a promising therapeutic strategy to target several solid carcinomas. We aimed to investigate the effects of glutaminolysis on cell proliferation in lung squamous cell carcinoma cell lines and to explore the potential of targeting glutaminolysis as an anticancer strategy. MATERIALS AND METHODS: Glutamine (Gln) dependence was assessed in six lung squamous cell carcinoma cell lines. Cell proliferation, mammalian target of rapamycin complex 1 (mTORC1) activity and the induction of autophagy were assessed after inhibition of glutaminolysis via Gln depletion or glutaminase (GLS) inhibition...
November 2016: Anticancer Research
https://www.readbyqxmd.com/read/27786281/synthesis-and-biological-evaluation-of-matrine-derivatives-containing-benzo-%C3%AE-pyrone-structure-as-potent-anti-lung-cancer-agents
#17
Lichuan Wu, Guizhen Wang, Shuaibing Liu, Jinrui Wei, Sen Zhang, Ming Li, Guangbiao Zhou, Lisheng Wang
Matrine, an active component of root extracts from Sophora flavescens Ait, is the main chemical ingredient of Fufang Kushen injection which was approved by Chinese FDA (CFDA) in 1995 as an anticancer drug to treat non-small cell lung cancer and liver cancer in combination with other anticancer drugs. Owning to its druggable potential, matrine is considered as an ideal lead compound for modification. We delineate herein the synthesis and anticancer effects of 17 matrine derivatives bearing benzo-α-pyrone structures...
October 27, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27782356/simvastatin-alleviates-airway-inflammation-and-remodelling-through-up-regulation-of-autophagy-in-mouse-models-of-asthma
#18
Wen Gu, Rong Cui, Tao Ding, Xiaoming Li, Juan Peng, Weiguo Xu, Fengfeng Han, Xuejun Guo
BACKGROUND AND OBJECTIVE: Statins have been widely used in inflammatory diseases including asthma, because of their anti-inflammatory and immunomodulatory properties. It has been shown that simvastatin induces autophagy and cell death in some circumstances. However, the possible cross-talk between simvastatin and autophagic processes in lung disease is largely unknown. Thus, we investigated the impact of simvastatin on airway inflammation and airway remodelling and the possible relationship of these processes to a simvastatin-induced autophagic pathway in mouse models of asthma...
October 26, 2016: Respirology: Official Journal of the Asian Pacific Society of Respirology
https://www.readbyqxmd.com/read/27779694/fentanyl-induces-autophagy-via-activation-of-the-ros-mapk-pathway-and-reduces-the-sensitivity-of-cisplatin-in-lung-cancer-cells
#19
Jiaqi Yao, Chi Ma, Wei Gao, Jinxiao Liang, Chang Liu, Hongfang Yang, Qiu Yan, Qingping Wen
Cancer pain is the most common complication of lung carcinoma. Opioid agonist fentanyl is widely used for relieving pain in cancer patients, and cisplatin (DDP)‑based chemotherapy is commonly used for the treatment of advanced lung cancer; these two drugs are always used together in lung carcinoma patients. However, the mechanisms and related biological pathways by which fentanyl influences cisplatin sensitivity are relatively poorly reported. Here, we found that fentanyl reduces the sensitivity of cisplatin in human lung cancer cells and induces autophagy...
October 19, 2016: Oncology Reports
https://www.readbyqxmd.com/read/27765907/activation-of-endoplasmic-reticulum-stress-promotes-autophagy-and-apoptosis-and-reverses-chemoresistance-of-human-small-cell-lung-cancer-cells-by-inhibiting-the-pi3k-akt-mtor-signaling-pathway
#20
Xin-Shuang Yu, Juan Du, Yu-Jun Fan, Feng-Jun Liu, Li-Li Cao, Ning Liang, De-Guo Xu, Jian-Dong Zhang
OBJECTIVE: This study aims to investigate the effects of endoplasmic reticulum stress (ERS) on autophagy, apoptosis and chemoresistance of human small cell lung cancer (SCLC) cells via the PI3K/AKT/mTOR signaling pathway. RESULTS: The expressions of ERS-related proteins (PEAK, eIF2α and CHOP) up-regulated, autophagy-related proteins (LC3, LC3-II and Beclin1) and apoptosis-related proteins (Bax and procaspase-3) down-regulated in NCI-H446 and H69 cells after tunicamycin treatment for 24 h...
October 18, 2016: Oncotarget
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