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https://www.readbyqxmd.com/read/29765383/fish-based-markers-enable-identification-of-chromosomes-derived-from-tetraploid-thinopyrum-elongatum-in-hybrid-lines
#1
Daiyan Li, Tinghui Li, Yanli Wu, Xiaohui Zhang, Wei Zhu, Yi Wang, Jian Zeng, Lili Xu, Xing Fan, Lina Sha, Haiqin Zhang, Yonghong Zhou, Houyang Kang
Tetraploid Thinopyrum elongatum , which has superior abiotic stress tolerance characteristics, and exhibits resistance to stripe rust, powdery mildew, and Fusarium head blight, is a wild relative of wheat and a promising source of novel genes for wheat improvement. Currently, a high-resolution Fluorescence in situ hybridization (FISH) karyotype of tetraploid Th. elongatum is not available. To develop chromosome-specific FISH-based markers, the hexaploid Trititrigia 8801 and two accessions of tetraploid Th. elongatum were characterized by different repetitive sequences probes...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29746580/characterization-and-mapping-of-leaf-rust-resistance-in-four-durum-wheat-cultivars
#2
Dhouha Kthiri, Alexander Loladze, P R MacLachlan, Amidou N'Diaye, Sean Walkowiak, Kirby Nilsen, Susanne Dreisigacker, Karim Ammar, Curtis J Pozniak
Widening the genetic basis of leaf rust resistance is a primary objective of the global durum wheat breeding effort at the International Wheat and Maize Improvement Center (CIMMYT). Breeding programs in North America are following suit, especially after the emergence of new races of Puccinia triticina such as BBG/BP and BBBQD in Mexico and the United States, respectively. This study was conducted to characterize and map previously undescribed genes for leaf rust resistance in durum wheat and to develop reliable molecular markers for marker-assisted breeding...
2018: PloS One
https://www.readbyqxmd.com/read/29744525/mapping-of-a-dominant-rust-resistance-gene-revealed-two-r-genes-around-the-major-rust_qtl-in-cultivated-peanut-arachis-hypogaea-l
#3
Suvendu Mondal, Anand M Badigannavar
A consensus rust QTL was identified within a 1.25 cM map interval of A03 chromosome in cultivated peanut. This map interval contains a TIR-NB-LRR R gene and four pathogenesis-related genes. Disease resistance in plants is manifested due to the specific interaction between the R gene product and its cognate avirulence gene product (AVR) in the pathogen. Puccinia arachidis Speg. causes rust disease and inflicts economic damages to peanut. Till now, no experimental evidence is known for the action of R gene in peanut for rust resistance...
May 9, 2018: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29743956/molecular-cytogenetic-identification-of-three-rust-resistant-wheat-thinopyrum-ponticum-partial-amphiploids
#4
Yanru Pei, Yu Cui, Yanping Zhang, Honggang Wang, Yinguang Bao, Xingfeng Li
Background: Thinopyrum ponticum (2n = 10× = 70, JS JS JS JS JJJJJJ) is an important wild perennial Triticeae species that has a unique gene pool with many desirable traits for common wheat. The partial amphiploids derived from wheat- Th. ponticum set up a bridge for transferring valuable genes from Th. ponticum into common wheat. Results: In this study, genomic in situ hybridization (GISH), multicolor GISH (mcGISH) and fluorescence in situ hybridization (FISH) were used to analyze the genomic constitution of SN0389, SN0398 and SN0406, three octoploid accessions with good resistance to rust...
2018: Molecular Cytogenetics
https://www.readbyqxmd.com/read/29740461/genome-wide-association-mapping-of-seedling-and-adult-plant-resistance-to-barley-stripe-rust-puccinia-striiformis-f-sp-hordei-in-india
#5
Andrea Visioni, Sanjaya Gyawali, Rajan Selvakumar, Om P Gangwar, Pradeep S Shekhawat, Subhash C Bhardwaj, Ayed M Al-Abdallat, Zakaria Kehel, Ramesh P S Verma
Barley stripe rust is caused by Puccinia striiformis f.sp. hordei , (Psh), occurs worldwide, and is a major disease in South Asia. The aim of this work was to identify and estimate effects of loci underlying quantitative resistance to rust at seedling and adult plant stages. HI-AM panel of 261 barley genotypes consisting of released cultivars from North and South America, Europe, Australia, advanced breeding lines, and local landraces from ICARDA barley program were screened at seedling and adult plant stages for resistance to Psh...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29728059/functional-analysis-of-a-pathogenesis-related-thaumatin-like-protein-gene-talr35pr5-from-wheat-induced-by-leaf-rust-fungus
#6
Jiarui Zhang, Fei Wang, Fang Liang, Yanjun Zhang, Lisong Ma, Haiyan Wang, Daqun Liu
BACKGROUND: Plants have evolved multifaceted defence mechanisms to resist pathogen infection. Production of the pathogenesis-related (PR) proteins in response to pathogen attack has been implicated in plant disease resistance specialized in systemic-acquired resistance (SAR). Our earlier studies have reported that a full length TaLr35PR5 gene, encoding a protein exhibiting amino acid and structural similarity to a sweet protein thaumatin, was isolated from wheat near-isogenic line TcLr35...
May 4, 2018: BMC Plant Biology
https://www.readbyqxmd.com/read/29696297/mapping-and-characterization-of-the-new-adult-plant-leaf-rust-resistance-gene-lr77-derived-from-santa-fe-winter-wheat
#7
James A Kolmer, Zhenqi Su, Amy Bernardo, Guihua Bai, Shiaoman Chao
A new gene for adult plant leaf rust resistance in wheat was mapped to chromosome 3BL. This gene was designated as Lr77. 'Santa Fe' is a hard red winter cultivar that has had long-lasting resistance to the leaf rust fungus, Puccinia triticina. The objective of this study was to determine the chromosome location of the adult plant leaf rust resistance in Santa Fe wheat. A partial backcross line of 'Thatcher' (Tc) wheat with adult plant leaf rust resistance derived from Santa Fe was crossed with Thatcher to develop a Thatcher//Tc*2/Santa Fe F6 recombinant inbred line (RIL) population...
April 25, 2018: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29674436/mapping-of-leaf-rust-resistance-genes-and-molecular-characterization-of-the-2ns-2as-translocation-in-the-wheat-cultivar-jagger
#8
Shulin Xue, James A Kolmer, Shuwen Wang, Liuling Yan
Winter wheat cultivar 'Jagger' was recently found to have an alien chromosomal segment 2NS that has Lr37 , a gene conferring resistance against leaf rust caused by Puccinia triticina The objective of this study was to map and characterize the gene(s) for seedling leaf rust resistance in Jagger. The recombinant inbred line (RIL) population of Jagger × '2174' was inoculated with leaf rust pathogen THBJG and BBBDB, and evaluated for infection type (IT) response. A major quantitative trait locus (QTL) for THBJG and BBBDB was coincidently mapped to chromosome arm 2AS, and the QTL accounted for 56...
April 19, 2018: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/29666883/snp-based-pool-genotyping-and-haplotype-analysis-accelerate-fine-mapping-of-the-wheat-genomic-region-containing-stripe-rust-resistance-gene-yr26
#9
Jianhui Wu, Qingdong Zeng, Qilin Wang, Shengjie Liu, Shizhou Yu, Jingmei Mu, Shuo Huang, Hanan Sela, Assaf Distelfeld, Lili Huang, Dejun Han, Zhensheng Kang
NGS-assisted super pooling emerging as powerful tool to accelerate gene mapping and haplotype association analysis within target region uncovering specific linkage SNPs or alleles for marker-assisted gene pyramiding. Conventional gene mapping methods to identify genes associated with important agronomic traits require significant amounts of financial support and time. Here, a single nucleotide polymorphism (SNP)-based mapping approach, RNA-Seq and SNP array assisted super pooling analysis, was used for rapid mining of a candidate genomic region for stripe rust resistance gene Yr26 that has been widely used in wheat breeding programs in China...
April 17, 2018: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29649208/assessing-the-durability-and-efficiency-of-landscape-based-strategies-to-deploy-plant-resistance-to-pathogens
#10
Loup Rimbaud, Julien Papaïx, Jean-François Rey, Luke G Barrett, Peter H Thrall
Genetically-controlled plant resistance can reduce the damage caused by pathogens. However, pathogens have the ability to evolve and overcome such resistance. This often occurs quickly after resistance is deployed, resulting in significant crop losses and a continuing need to develop new resistant cultivars. To tackle this issue, several strategies have been proposed to constrain the evolution of pathogen populations and thus increase genetic resistance durability. These strategies mainly rely on varying different combinations of resistance sources across time (crop rotations) and space...
April 12, 2018: PLoS Computational Biology
https://www.readbyqxmd.com/read/29648947/pyramiding-for-resistance-durability-theory-and-practice
#11
Chris Mundt
Durable disease resistance is a key component of global food security, and combining resistance genes into "pyramids" is an important way to increase durability of resistance. The mechanisms by which pyramids impact durability are not well known. The traditional view of resistance pyramids considers the use of major resistance gene (R-gene) combinations deployed against pathogens that are primarily asexual. Interestingly, published examples of the successful use of pyramids in the traditional sense are rare...
April 12, 2018: Phytopathology
https://www.readbyqxmd.com/read/29619038/optimized-use-of-low-depth-genotyping-by-sequencing-for-genomic-prediction-among-multi-parental-family-pools-and-single-plants-in-perennial-ryegrass-lolium-perenne-l
#12
Fabio Cericola, Ingo Lenk, Dario Fè, Stephen Byrne, Christian S Jensen, Morten G Pedersen, Torben Asp, Just Jensen, Luc Janss
Ryegrass single plants, bi-parental family pools, and multi-parental family pools are often genotyped, based on allele-frequencies using genotyping-by-sequencing (GBS) assays. GBS assays can be performed at low-coverage depth to reduce costs. However, reducing the coverage depth leads to a higher proportion of missing data, and leads to a reduction in accuracy when identifying the allele-frequency at each locus. As a consequence of the latter, genomic relationship matrices (GRMs) will be biased. This bias in GRMs affects variance estimates and the accuracy of GBLUP for genomic prediction (GBLUP-GP)...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29616061/identifying-quantitative-trait-loci-qtls-and-developing-diagnostic-markers-linked-to-orange-rust-resistance-in-sugarcane-saccharum-spp
#13
Xiping Yang, Md S Islam, Sushma Sood, Stephanie Maya, Erik A Hanson, Jack Comstock, Jianping Wang
Sugarcane ( Saccharum spp.) is an important economic crop, contributing up to 80% of table sugar used in the world and has become a promising feedstock for biofuel production. Sugarcane production has been threatened by many diseases, and fungicide applications for disease control have been opted out for sustainable agriculture. Orange rust is one of the major diseases impacting sugarcane production worldwide. Identifying quantitative trait loci (QTLs) and developing diagnostic markers are valuable for breeding programs to expedite release of superior sugarcane cultivars for disease control...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29614079/identification-and-characterization-of-wheat-stem-rust-resistance-gene-sr21-effective-against-the-ug99-race-group-at-high-temperature
#14
Shisheng Chen, Wenjun Zhang, Stephen Bolus, Matthew N Rouse, Jorge Dubcovsky
Wheat stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), is a devastating foliar disease. The Ug99 race group has combined virulence to most stem rust (Sr) resistance genes deployed in wheat and is a threat to global wheat production. Here we identified a coiled-coil, nucleotide-binding leucine-rich repeat protein (NLR) completely linked to the Ug99 resistance gene Sr21 from Triticum monococcum. Loss-of-function mutations and transgenic complementation confirmed that this gene is Sr21. Sr21 transcripts were significantly higher at high temperatures, and this was associated with significant upregulation of pathogenesis related (PR) genes and increased levels of resistance at those temperatures...
April 2018: PLoS Genetics
https://www.readbyqxmd.com/read/29600262/the-stabilising-effect-of-the-anterior-oblique-ligament-to-prevent-directional-subluxation-at-the-trapeziometacarpal-joint-of-the-thumb-a-biomechanical-cadaveric-study
#15
Mark R McCann, Philippa A Rust, Robert Wallace
Background: The trapeziometacarpal joint (TMCJ) is inherently unstable, relying on ligament restraint to prevent subluxation. Subluxation of the thumb in a dorsoradial direction is often observed in clinical practice, either after acute ligament injury or more commonly with osteoarthritis (OA). This subluxation follows loss of function of trapeziometacarpal ligaments that stabilise this joint, resisting the deforming force of abductor pollicis longus (APL). The exact ligaments that stabilise and prevent the thumb from the pull of APL causing dorsoradial subluxation remain unknown, although the anterior oblique ligament (AOL) has been implicated...
March 2018: Archives of Bone and Joint Surgery
https://www.readbyqxmd.com/read/29589041/genome-wide-association-mapping-for-resistance-to-leaf-rust-stripe-rust-and-tan-spot-in-wheat-reveals-potential-candidate-genes
#16
Philomin Juliana, Ravi P Singh, Pawan K Singh, Jesse A Poland, Gary C Bergstrom, Julio Huerta-Espino, Sridhar Bhavani, Jose Crossa, Mark E Sorrells
Genome-wide association mapping in conjunction with population sequencing map and Ensembl plants was used to identify markers/candidate genes linked to leaf rust, stripe rust and tan spot resistance in wheat. Leaf rust (LR), stripe rust (YR) and tan spot (TS) are some of the important foliar diseases in wheat (Triticum aestivum L.). To identify candidate resistance genes for these diseases in CIMMYT's (International Maize and Wheat Improvement Center) International bread wheat screening nurseries, we used genome-wide association studies (GWAS) in conjunction with information from the population sequencing map and Ensembl plants...
March 27, 2018: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29564500/high-throughput-genotyping-by-sequencing-facilitates-molecular-tagging-of-a-novel-rust-resistance-gene-r-15-in-sunflower-helianthus-annuus-l
#17
G J Ma, Q J Song, S G Markell, L L Qi
A novel rust resistance gene, R15 , derived from the cultivated sunflower HA-R8 was assigned to linkage group 8 of the sunflower genome using a genotyping-by-sequencing approach. SNP markers closely linked to R15 were identified, facilitating marker-assisted selection of resistance genes. The rust virulence gene is co-evolving with the resistance gene in sunflower, leading to the emergence of new physiologic pathotypes. This presents a continuous threat to the sunflower crop necessitating the development of resistant sunflower hybrids providing a more efficient, durable, and environmentally friendly host plant resistance...
March 21, 2018: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29560515/characterisation-and-mapping-of-adult-plant-stripe-rust-resistance-in-wheat-accession-aus27284
#18
Vallence Nsabiyera, Harbans S Bariana, Naeela Qureshi, Debbie Wong, Matthew J Hayden, Urmil K Bansal
A new adult plant stripe rust resistance gene, Yr80, was identified in a common wheat landrace Aus27284. Linked markers were developed and validated for their utility in marker-assisted selection. Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is among the most important constraints to global wheat production. The identification and characterisation of new sources of host plant resistance enrich the gene pool and underpin deployment of resistance gene pyramids in new cultivars. Aus27284 exhibited resistance at the adult plant stage against predominant Pst pathotypes and was crossed with a susceptible genotype Avocet S...
March 20, 2018: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29559535/comparative-transcriptomics-among-four-white-pine-species
#19
Ethan A G Baker, Jill L Wegrzyn, Uzay U Sezen, Taylor Falk, Patricia E Maloney, Detlev R Vogler, Annette Delfino-Mix, Camille Jensen, Jeffry Mitton, Jessica Wright, Brian Knaus, Hardeep Rai, Richard Cronn, Daniel Gonzalez-Ibeas, Hans A Vasquez-Gross, Randi A Famula, Jun-Jun Liu, Lara M Kueppers, David B Neale
Conifers are the dominant plant species throughout the high latitude boreal forests as well as some lower latitude temperate forests of North America, Europe, and Asia. As such, they play an integral economic and ecological role across much of the world. This study focused on the characterization of needle transcriptomes from four ecologically important and understudied North American white pines within the Pinus subgenus Strobus. The populations of many Strobus species are challenged by native and introduced pathogens, native insects, and abiotic factors...
March 20, 2018: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/29541084/transcriptomic-analysis-reveal-the-molecular-mechanisms-of-wheat-higher-temperature-seedling-plant-resistance-to-puccinia-striiformis-f-sp-tritici
#20
Fei Tao, Junjuan Wang, Zhongfeng Guo, Jingjing Hu, Xiangming Xu, Jiarong Yang, Xianming Chen, Xiaoping Hu
Stripe rust, caused by Puccinia striiformis f. sp. tritici ( Pst ), is a destructive disease of wheat worldwide. The disease is preferably controlled by growing resistant cultivars. Wheat cultivar Xiaoyan 6 (XY 6) has been resistant to stripe rust since its release. In the previous studies, XY 6 was found to have higher-temperature seedling-plant (HTSP) resistance. However, the molecular mechanisms of HTSP resistance were not clear. To identify differentially expressed genes (DEGs) involved in HTSP resistance, we sequenced 30 cDNA libraries constructed from XY 6 seedlings exposed to several temperature treatments...
2018: Frontiers in Plant Science
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