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https://www.readbyqxmd.com/read/29140770/specificity-and-genetic-diversity-of-xylose-fermenting-scheffersomyces-yeasts-associated-with-small-blue-stag-beetles-of-the-genus-platycerus-in-east-asia
#1
Masahiko Tanahashi, Jong-Kuk Kim, Kana Watanabe, Takema Fukatsu, Kôhei Kubota
Among insect-fungus relationships, xylose-fermenting Scheffersomyces yeasts are well known for their potential in utilizing wood hemicelluloses and their association with various wood-feeding insects. However, their specificity to host insects or strain-level diversity within host species has not been clearly elucidated. In the insect family Lucanidae, larvae usually feed on decaying wood, and adult females consistently possess a fungus-storage organ, called the mycangium, near the abdominal tip. Here the authors investigated host-symbiont relationships between Scheffersomyces yeast symbionts and small blue stag beetles of the genus Platycerus (Coleoptera: Lucanidae) in East Asia by using intergenic spacer (IGS) region as a genetic marker...
November 9, 2017: Mycologia
https://www.readbyqxmd.com/read/29104233/the-complete-amomum-kravanh-chloroplast-genome-sequence-and-phylogenetic-analysis-of-the-commelinids
#2
Mingli Wu, Qing Li, Zhigang Hu, Xiwen Li, Shilin Chen
Amomum kravanh is an important edible and medicinal herb, the dried fruits of which are widely used in traditional herbal medicine as cardamom. We sequenced and analyzed the complete chloroplast (cp) genome of A. kravanh with herbgenomics technologies. The size of the A. kravanh cp genome was 162,766 bp, which consisted of long (LSC; 87,728 bp) and short (SSC; 15,390 bp) single-copy regions, separated by a pair of inverted repeats (IRs; 29,824 bp). The genome encoded 114 unique genes, including 80 protein-coding genes, 30 tRNAs and four rRNAs...
November 1, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29057217/characterization-and-distribution-of-the-autb-gene-in-neisseria-meningitidis
#3
Aiyu Zhang, Pan Zhao, Bingqing Zhu, Fenglin Shi, Li Xu, Yuan Gao, Na Xie, Zhujun Shao
We aimed to investigate and understand the characterization and distribution of the autB gene in Neisseria meningitidis in China. autB is flanked by two conservative genes, smpB and glcD, and it can be present in the majority of meningococcal isolates, but not in 053442 of clonal complex 4821 (CC4821) which contains a 968 bp sequence. In this study, we sequenced the intervenient region between smpB and glcD in 178 Chinese N. meningitidis strains isolated from both patients and carriers. There were 110 serogroupable strains, other 68 were non-groupable (NG)...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29047380/population-genetics-of-enterocytozoon-bieneusi-in-captive-giant-pandas-of-china
#4
Wei Li, Yuan Song, Zhijun Zhong, Xiangming Huang, Chengdong Wang, Caiwu Li, Haidi Yang, Haifeng Liu, Zhihua Ren, Jingchao Lan, Kongju Wu, Guangneng Peng
BACKGROUND: Most studies on Enterocytozoon bieneusi are conducted based on the internal transcribed spacer (ITS) region of the rRNA gene, whereas some have examined E. bieneusi population structures. Currently, the population genetics of this pathogen in giant panda remains unknown. The objective of this study was to determine the E. bieneusi population in captive giant pandas in China. RESULTS: We examined 69 E. bieneusi-positive specimens from captive giant pandas in China using five loci (ITS, MS1, MS3, MS4 and MS7) to infer E...
October 18, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28986641/first-detection-of-rickettsia-conorii-ssp-caspia-in-rhipicephalus-sanguineus-in-zambia
#5
Lidia Chitimia-Dobler, Gerhard Dobler, Sabine Schaper, Thomas Küpper, Simone Kattner, Silke Wölfel
Ticks are important vectors for Rickettsia spp. of the spotted fever group all around the world. Rickettsia conorii is the etiological agent of boutonneuse fever in the Mediterranean region and Africa. Tick identification was based on morphological features and further characterized using the 16S rRNA gene. The ticks were individually tested using pan-Rickettsia real-time-PCR for screening, and 23S-5S intergenic spacer region, 16S rDNA, gltA, sca4, ompB, and ompA genes were used to analyze the Rickettsia positive samples...
November 2017: Parasitology Research
https://www.readbyqxmd.com/read/28975409/diversity-and-prevalence-of-bartonella-species-in-small-mammals-from-slovakia-central-europe
#6
Eva Špitalská, Lenka Minichová, Elena Kocianová, Ľudovít Škultéty, Lenka Mahríková, Zuzana Hamšíková, Mirko Slovák, Mária Kazimírová
Wild-living rodents are important hosts for zoonotic pathogens. Bartonella infections are widespread in rodents; however, in Slovakia, knowledge on the prevalence of these bacteria in small mammals is limited. We investigated the prevalence and diversity of Bartonella species in the spleens of 640 rodents of six species (Apodemus flavicollis, Apodemus sylvaticus, Myodes glareolus, Microtus arvalis, Microtus subterraneus, and Micromys minutus) and in the European mole (Talpa europaea) from three different habitat types in south-western and central Slovakia...
November 2017: Parasitology Research
https://www.readbyqxmd.com/read/28966259/phylogenic-analysis-and-evaluation-of-ephedra-plants-and-aconites-for-medicinal-use
#7
Nobuko Kakiuchi
Although Kampo medicine is now fully integrated into the modern Japanese healthcare system, most Kampo formulations depend on imported crude drugs from limited foreign areas. To prepare for possible shortages of crude drugs in the future, a wider scope for the supply of medicinal plants is necessary. We conducted field research and collaborated with international laboratories for phylogenic analysis and evaluation of medicinal plant resources. Our research on ephedra plants from a wide region of Eurasia has, for example, confirmed their phylogenic structure: based on DNA sequencing analysis of nuclear ribosomal internal transcribed spacer region 1 (ITS1) as well as the chloroplast intergenic spacer between trnL and trnF (trnL-F), the 8 major Chinese species and related plants grown on the continent could be divided into 3 groups...
2017: Yakugaku Zasshi: Journal of the Pharmaceutical Society of Japan
https://www.readbyqxmd.com/read/28964561/-identification-of-species-of-mycoplasma-and-ureaplasma-diversum-from-argentinian-dairy-herds
#8
Camila Sosa, Liliana Tirante, Javier Chaves, Martín Pol, Arnaldo Ambrogi, José Angel Giraudo, Pablo Tamiozzo
Several species of Mycoplasma and Ureaplasma diversum can cause diseases in dairy cattle, which can be associated or not with clinical manifestations. In our country, the presence of Mycoplasma bovis, Mycoplasma californicum and Mycoplasma canadense has been detected, being the only mycoplasma species identified so far. The objective of this study was to identify other species of the Mycoplasmataceae family. Thirty-five Mycoplasma spp.-like isolates obtained from different samples from cattle, with or without clinical symptoms, from eight herds located in the provinces of Santa Fe, Cordoba, Buenos Aires and San Luis were utilized in the present study...
September 27, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/28962847/complete-sequence-and-characterization-of-the-ectropis-oblique-mitochondrial-genome-and-its-phylogenetic-implications
#9
Li-Shang Dai, Saima Kausar, Muhammad Nadeem Abbas, Tian-Tian Wang
In the present study, we sequenced the entire mitochondrial genome (mitogenome) of Ectropis oblique using PCR amplification and sequencing methods The entire mitogenome is 15,356bp long, including 13 protein coding genes, 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes and A+T rich element. The base composition and gene arrangement are identical to those of other lepidopterans. Of these 37 genes, twenty three are resided on heavy strand, while fourteen located on light strand. The newly sequenced mitogenome displayed a biased Adenine/thymine 84...
September 26, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28927427/first-molecular-detection-and-characterization-of-zoonotic-bartonella-species-in-fleas-infesting-domestic-animals-in-tunisia
#10
Saba Zouari, Fatma Khrouf, Youmna M'ghirbi, Ali Bouattour
BACKGROUND: Bartonellosis is an emerging vector-borne disease caused by different intracellular bacteria of the genus Bartonella (Rhizobiales: Bartonellaceae) that is transmitted primarily by blood-sucking arthropods such as sandflies, ticks and fleas. In Tunisia, there are no data available identifying the vectors of Bartonella spp. In our research, we used molecular methods to detect and characterize Bartonella species circulating in fleas collected from domestic animals in several of the country's bioclimatic areas...
September 19, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28912548/phylogenetic-implications-of-nuclear-rrna-igs-variation-in-stipa-l-poaceae
#11
Katarzyna Krawczyk, Marcin Nobis, Arkadiusz Nowak, Monika Szczecińska, Jakub Sawicki
The article takes up the problem of deficiency of molecular marker, which could illustrate molecular variability as well as phylogenetic relation within the genus of Stipa L. (Poaceae). Researches made so far hadn't delivered sufficient information about relationships between particular taxa from the genus of Stipa. In the present study, we analyzed variability and phylogenetic informativeness of nuclear ribosomal DNA in six species from the genus against five other species from Poaceae including a division of this region into functional elements and domains...
September 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28911667/dna-barcode-and-identification-of-the-varieties-and-provenances-of-taiwan-s-domestic-and-imported-made-teas-using-ribosomal-internal-transcribed-spacer-2-sequences
#12
Shih-Chieh Lee, Chia-Hsiang Wang, Cheng-En Yen, Chieh Chang
The major aim of made tea identification is to identify the variety and provenance of the tea plant. The present experiment used 113 tea plants [Camellia sinensis (L.) O. Kuntze] housed at the Tea Research and Extension Substation, from which 113 internal transcribed spacer 2 (ITS2) fragments, 104 trnL intron, and 98 trnL-trnF intergenic sequence region DNA sequences were successfully sequenced. The similarity of the ITS2 nucleotide sequences between tea plants housed at the Tea Research and Extension Substation was 0...
April 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28911333/profiling-bacterial-communities-by-minion-sequencing-of-ribosomal-operons
#13
Lee J Kerkhof, Kevin P Dillon, Max M Häggblom, Lora R McGuinness
BACKGROUND: An approach utilizing the long-read capability of the Oxford Nanopore MinION to rapidly sequence bacterial ribosomal operons of complex natural communities was developed. Microbial fingerprinting employs domain-specific forward primers (16S rRNA subunit), reverse primers (23S rRNA subunit), and a high-fidelity Taq polymerase with proofreading capabilities. Amplicons contained both ribosomal subunits for broad-based phylogenetic assignment (~ 3900 bp of sequence), plus the intergenic spacer (ITS) region (~ 300 bp) for potential strain-specific identification...
September 15, 2017: Microbiome
https://www.readbyqxmd.com/read/28886724/composition-of-anopheles-mosquitoes-their-blood-meal-hosts-and-plasmodium-falciparum-infection-rates-in-three-islands-with-disparate-bed-net-coverage-in-lake-victoria-kenya
#14
Edwin Ogola, Jandouwe Villinger, Danspaid Mabuka, David Omondi, Benedict Orindi, James Mutunga, Vincent Owino, Daniel K Masiga
BACKGROUND: Small islands serve as potential malaria reservoirs through which new infections might come to the mainland and may be important targets in malaria elimination efforts. This study investigated malaria vector species diversity, blood-meal hosts, Plasmodium infection rates, and long-lasting insecticidal net (LLIN) coverage on Mageta, Magare and Ngodhe Islands of Lake Victoria in western Kenya, a region where extensive vector control is implemented on the mainland. RESULTS: From trapping for six consecutive nights per month (November 2012 to March 2015) using CDC light traps, pyrethrum spray catches and backpack aspiration, 1868 Anopheles mosquitoes were collected...
September 8, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28874137/mitochondrial-genomes-of-blister-beetles-coleoptera-meloidae-and-two-large-intergenic-spacers-in-hycleus-genera
#15
Chao Du, Lifang Zhang, Ting Lu, Jingnan Ma, Chenjuan Zeng, Bisong Yue, Xiuyue Zhang
BACKGROUND: Insect mitochondrial genomes (mitogenomes) exhibit high diversity in some lineages. The gene rearrangement and large intergenic spacer (IGS) have been reported in several Coleopteran species, although very little is known about mitogenomes of Meloidae. RESULTS: We determined complete or nearly complete mitogenomes of seven meloid species. The circular genomes encode 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs) and two ribosomal RNAs (rRNAs), and contain a control region, with gene arrangement identical to the ancestral type for insects...
September 6, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28817651/comparative-genetic-analysis-of-the-45s-rdna-intergenic-spacers-from-three-saccharum-species
#16
COMPARATIVE STUDY
Yongji Huang, Fan Yu, Xueting Li, Ling Luo, Jiayun Wu, Yongqing Yang, Zuhu Deng, Rukai Chen, Muqing Zhang
The 45S ribosomal DNA (rDNA) units are separated by an intergenic spacer (IGS) containing the signals for transcription and processing of rRNAs. For the first time, we sequenced and analyzed the entire IGS region from three original species within the genus Saccharum, including S. spontaneum, S. robustum, and S. officinarum in this study. We have compared the IGS organization within three original species of the genus Saccharum. The IGS of these three original species showed similar overall organizations comprised of putative functional elements needed for rRNA gene activity as well as a non-transcribed spacer (NTS), a promoter region, and an external transcribed spacer (ETS)...
2017: PloS One
https://www.readbyqxmd.com/read/28800082/complete-chloroplast-genome-sequence-and-phylogenetic-analysis-of-the-medicinal-plant-artemisia-annua
#17
Xiaofeng Shen, Mingli Wu, Baosheng Liao, Zhixiang Liu, Rui Bai, Shuiming Xiao, Xiwen Li, Boli Zhang, Jiang Xu, Shilin Chen
The complete chloroplast genome of Artemisia annua (Asteraceae), the primary source of artemisinin, was sequenced and analyzed. The A. annua cp genome is 150,995 bp, and harbors a pair of inverted repeat regions (IRa and IRb), of 24,850 bp each that separate large (LSC, 82,988 bp) and small (SSC, 18,267 bp) single-copy regions. Our annotation revealed that the A. annua cp genome contains 113 genes and 18 duplicated genes. The gene order in the SSC region of A. annua is inverted; this fact is consistent with the sequences of chloroplast genomes from three other Artemisia species...
August 11, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28791039/impact-of-transgenic-brassica-napus-harboring-the-antifungal-synthetic-chitinase-nic-gene-on-rhizosphere-microbial-diversity-and-enzyme-activities
#18
Mohammad S Khan, Syed U Sadat, Asad Jan, Iqbal Munir
Transgenic Brassica napus harboring the synthetic chitinase (NiC) gene exhibits broad-spectrum antifungal resistance. As the rhizosphere microorganisms play an important role in element cycling and nutrient transformation, therefore, biosafety assessment of NiC containing transgenic plants on soil ecosystem is a regulatory requirement. The current study is designed to evaluate the impact of NiC gene on the rhizosphere enzyme activities and microbial community structure. The transgenic lines with the synthetic chitinase gene (NiC) showed resistance to Alternaria brassicicola, a common disease causing fungal pathogen...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28790364/chloroplast-genomes-of-arabidopsis-halleri-ssp-gemmifera-and-arabidopsis-lyrata-ssp-petraea-structures-and-comparative-analysis
#19
Sajjad Asaf, Abdul Latif Khan, Muhammad Aaqil Khan, Muhammad Waqas, Sang-Mo Kang, Byung-Wook Yun, In-Jung Lee
We investigated the complete chloroplast (cp) genomes of non-model Arabidopsis halleri ssp. gemmifera and Arabidopsis lyrata ssp. petraea using Illumina paired-end sequencing to understand their genetic organization and structure. Detailed bioinformatics analysis revealed genome sizes of both subspecies ranging between 154.4~154.5 kbp, with a large single-copy region (84,197~84,158 bp), a small single-copy region (17,738~17,813 bp) and pair of inverted repeats (IRa/IRb; 26,264~26,259 bp). Both cp genomes encode 130 genes, including 85 protein-coding genes, eight ribosomal RNA genes and 37 transfer RNA genes...
August 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28763486/comparative-analysis-of-complete-plastid-genomes-from-wild-soybean-glycine-soja-and-nine-other-glycine-species
#20
COMPARATIVE STUDY
Sajjad Asaf, Abdul Latif Khan, Muhammad Aaqil Khan, Qari Muhammad Imran, Sang-Mo Kang, Khdija Al-Hosni, Eun Ju Jeong, Ko Eun Lee, In-Jung Lee
The plastid genomes of different plant species exhibit significant variation, thereby providing valuable markers for exploring evolutionary relationships and population genetics. Glycine soja (wild soybean) is recognized as the wild ancestor of cultivated soybean (G. max), representing a valuable genetic resource for soybean breeding programmes. In the present study, the complete plastid genome of G. soja was sequenced using Illumina paired-end sequencing and then compared it for the first time with previously reported plastid genome sequences from nine other Glycine species...
2017: PloS One
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