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intergenic spacer region

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https://www.readbyqxmd.com/read/27933047/diazotroph-diversity-in-the-sea-ice-melt-ponds-and-surface-waters-of-the-eurasian-basin-of-the-central-arctic-ocean
#1
Mar Fernández-Méndez, Kendra A Turk-Kubo, Pier L Buttigieg, Josephine Z Rapp, Thomas Krumpen, Jonathan P Zehr, Antje Boetius
The Eurasian basin of the Central Arctic Ocean is nitrogen limited, but little is known about the presence and role of nitrogen-fixing bacteria. Recent studies have indicated the occurrence of diazotrophs in Arctic coastal waters potentially of riverine origin. Here, we investigated the presence of diazotrophs in ice and surface waters of the Central Arctic Ocean in the summer of 2012. We identified diverse communities of putative diazotrophs through targeted analysis of the nifH gene, which encodes the iron protein of the nitrogenase enzyme...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27924535/targeting-conserved-genes-in-fusarium-species
#2
Jéssica Gil-Serna, Belén Patiño, Miguel Jurado, Salvador Mirete, Covadonga Vázquez, M Teresa González-Jaén
Fumonisins are important mycotoxins contaminating foods and feeds which are mainly produced by F. verticillioides and F. proliferatum. Additionally, both are pathogens of maize and other cereals. We describe two highly sensitive, rapid, and species-specific PCR protocols which enable detection and discrimination of these closely related species in cereal flour or grain samples. The specific primer pairs of these assays were based on the intergenic spacer region of the multicopy rDNA unit which highly improves the sensitivity of the PCR assay in comparison with single-copy target regions...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27917544/postvaccination-wounds-associated-predominantly-with-arcanobacterium-phocae-in-minks-neovison-vison-at-three-mink-farms
#3
Robert J Molenaar, Rianne Buter, Agnieszka Sroka
BACKGROUND: The emerging skin disease fur animal epidemic necrotic pyoderma (FENP) has been attributed to infection with Arcanobacterium phocae (ABP). The exact pathogenesis and risk factors of FENP have yet to be elucidated. ANIMALS: Three mink from each of three different mink farms (A-C) with postvaccination skin wounds at the vaccination site and six mink from an unaffected mink farm (D) that had used the same vaccine batch and vaccination site (hind leg). METHODS AND RESULTS: All minks from farms A-C had severe necrotizing to necropurulent dermatitis where they were vaccinated intramuscularly in the hind leg...
December 4, 2016: Veterinary Dermatology
https://www.readbyqxmd.com/read/27917315/the-first-complete-plastid-genomes-of-melastomataceae-are-highly-structurally-conserved
#4
Marcelo Reginato, Kurt M Neubig, Lucas C Majure, Fabian A Michelangeli
BACKGROUND: In the past three decades, several studies have predominantly relied on a small sample of the plastome to infer deep phylogenetic relationships in the species-rich Melastomataceae. Here, we report the first full plastid sequences of this family, compare general features of the sampled plastomes to other sequenced Myrtales, and survey the plastomes for highly informative regions for phylogenetics. METHODS: Genome skimming was performed for 16 species spread across the Melastomataceae...
2016: PeerJ
https://www.readbyqxmd.com/read/27834904/chemical-and-genetic-diversity-of-nodularia-spumigena-from-the-baltic-sea
#5
Hanna Mazur-Marzec, Mireia Bertos-Fortis, Anna Toruńska-Sitarz, Anna Fidor, Catherine Legrand
Nodularia spumigena is a toxic, filamentous cyanobacterium occurring in brackish waters worldwide, yet forms extensive recurrent blooms in the Baltic Sea. N. spumigena produces several classes of non-ribosomal peptides (NRPs) that are active against several key metabolic enzymes. Previously, strains from geographically distant regions showed distinct NRP metabolic profiles. In this work, conspecific diversity in N. spumigena was studied using chemical and genetic approaches. NRP profiles were determined in 25 N...
November 10, 2016: Marine Drugs
https://www.readbyqxmd.com/read/27818123/identification-and-molecular-survey-of-borrelia-burgdorferi-sensu-lato-in-sika-deer-cervus-nippon-from-jilin-province-north-eastern-china
#6
Bintao Zhai, Qingli Niu, Jifei Yang, Zhijie Liu, Junlong Liu, Hong Yin, Qiaoying Zeng
Lyme disease caused by Borrelia burgdorferi sensu lato (s.l.) is a common disease of domestic animals and wildlife worldwide. Sika deer is first-grade state-protected wildlife animals in China and have economic consequences for humans. It is reported that sika deer may serve as an important reservoir host for several species of B. burgdorferi s.l. and may transmit these species to humans and animals. However, little is known about the presence of Borrelia pathogens in sika deer in China. In this study, the existence and prevalence of Borrelia sp...
November 3, 2016: Acta Tropica
https://www.readbyqxmd.com/read/27813572/chloroplast-dna-analysis-of-tunisian-cork-oak-populations-quercus-suber-l-sequence-variations-and-molecular-evolution-of-the-trnl-uaa-trnf-gaa-region
#7
A Abdessamad, G Baraket, H Sakka, Y Ammari, M Ksontini, A Salhi Hannachi
Sequences of the trnL-trnF spacer and combined trnL-trnF region in chloroplast DNA of cork oak (Quercus suber L.) were analyzed to detect polymorphisms and to elucidate molecular evolution and demographic history. The aligned sequences varied in length and nucleotide composition. The overall ratio of transition/transversion (ti/tv) of 0.724 for the intergenic spacer and 0.258 for the pooled sequences were estimated, and indicated that transversions are more frequent than transitions. The molecular evolution and demographic history of Q...
October 24, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27790066/taxonomic-characterization-evaluation-of-toxigenicity-and-saccharification-capability-of-aspergillus-section-flavi-isolates-from-korean-traditional-wheat-based-fermentation-starter-nuruk
#8
Jyotiranjan Bal, Suk-Hyun Yun, Jeesun Chun, Beom-Tae Kim, Dae-Hyuk Kim
The most economically important species used in a wide range of fermentation industries throughout Asia belong to Aspergillus section Flavi, which are morphologically and phylogenetically indistinguishable, with a few being toxigenic and therefore a major concern. They are frequently isolated from Korean fermentation starters, such as nuruk and meju. The growing popularity of traditional Korean alcoholic beverages has led to a demand for their quality enhancement, therefore requiring selection of efficient non-toxigenic strains to assist effective fermentation...
September 2016: Mycobiology
https://www.readbyqxmd.com/read/27788060/detection-and-characterization-of-the-emerging-relapsing-fever-pathogen-borrelia-miyamotoi-from-the-ixodes-ricinus-tick-in-the-rural-trakya-thrace-region-of-northwestern-turkey
#9
Keiko Sakakibara, Ece Şen, Kozue Sato, Hiroki Kawabata, Norio Ohashi, Toshiyuki Masuzawa
The hard tick-borne relapsing fever agent, Borrelia miyamotoi infection in Ixodes ricinus ticks sampled from Istanbul and the countryside of Kirklareli in northwestern Turkey, was examined by TaqMan-PCR targeting 16S rDNA, nested PCR targeting 16S rDNA, the flagellin gene (flaB), and the 16S and 23S rDNA intergenic spacer (IGS), and sequencing analyses of these amplicons. B. miyamotoi was detected in 1 out of 248 I. ricinus ticks (infection rate 0.4%). The tick infected with B. miyamotoi was collected in Longos, Kirklareli province on the European side of Turkey near the Bulgarian border...
December 2016: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/27703667/rapid-and-high-throughput-molecular-identification-of-diverse-mosquito-species-by-high-resolution-melting-analysis
#10
Yvonne Ukamaka Ajamma, Enock Mararo, David Omondi, Thomas Onchuru, Anne W T Muigai, Daniel Masiga, Jandouwe Villinger
Mosquitoes are a diverse group of invertebrates, with members that are among the most important vectors of diseases. The correct identification of mosquitoes is paramount to the control of the diseases that they transmit. However, morphological techniques depend on the quality of the specimen and often unavailable taxonomic expertise, which may still not be able to distinguish mosquitoes among species complexes (sibling and cryptic species). High resolution melting (HRM) analyses, a closed-tube, post-polymerase chain reaction (PCR) method used to identify variations in nucleic acid sequences, has been used to differentiate species within the Anopheles gambiae and Culex pipiens complexes...
2016: F1000Research
https://www.readbyqxmd.com/read/27668995/community-structure-population-dynamics-and-diversity-of-fungi-in-a-full-scale-membrane-bioreactor-mbr-for-urban-wastewater-treatment
#11
P Maza-Márquez, R Vilchez-Vargas, F M Kerckhof, E Aranda, J González-López, B Rodelas
Community structure, population dynamics and diversity of fungi were monitored in a full-scale membrane bioreactor (MBR) operated throughout four experimental phases (Summer 2009, Autumn 2009, Summer 2010 and Winter, 2012) under different conditions, using the 18S-rRNA gene and the intergenic transcribed spacer (ITS2-region) as molecular markers, and a combination of temperature-gradient gel electrophoresis and 454-pyrosequencing. Both total and metabolically-active fungal populations were fingerprinted, by amplification of molecular markers from community DNA and retrotranscribed RNA, respectively...
November 15, 2016: Water Research
https://www.readbyqxmd.com/read/27629063/tectonic-and-climatic-impacts-on-the-biota-within-the-red-river-fault-evidence-from-phylogeography-of-cycas-dolichophylla-cycadaceae
#12
Ying Zheng, Jian Liu, Xun Gong
Dramatic crustal deformation and river incision in Southwest China induced by the Indo-Asian collision have long been argued to contribute to the complicated landscapes, heterogeneous environment and abundant biodiversity in this region. However, biological impacts in promoting intraspecific phylogeographical subdivision and divergence along the Red River Fault zone (RRF) remain poorly understood. To investigate the possible biological effects of tectonic movements and environment variations within the RRF, the phylogeography of Cycas dolichophylla-an endemic but widely distributed Cycas in Southwest China and North Vietnam along the RRF were carried out based on four chloroplast DNA intergenic spacers (cpDNA), three nuclear DNA sequences (nDNA) and 16 simple sequence repeat variations (SSR)...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27618991/structural-differences-in-gut-bacteria-communities-in-developmental-stages-of-natural-populations-of-lutzomyia-evansi-from-colombia-s-caribbean-coast
#13
Rafael José Vivero, Natalia Gil Jaramillo, Gloria Cadavid-Restrepo, Sandra I Uribe Soto, Claudia Ximena Moreno Herrera
BACKGROUND: Lutzomyia evansi, a phlebotomine insect endemic to Colombia's Caribbean coast, is considered to be the main vector of visceral and cutaneous leishmaniasis in the region. Although insects of this species can harbor pathogenic and non-pathogenic microorganisms in their intestinal microbiota, there is little information available about the diversity of gut bacteria present in Lutzomyia evansi. In this study, conventional microbiological methods and molecular tools were used to assess the composition of bacterial communities associated with Lutzomyia evansi guts in immature and adult stages of natural populations from the department of Sucre (Caribbean coast of Colombia)...
2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27576718/complete-sequence-construction-of-the-highly-repetitive-ribosomal-rna-gene-repeats-in-eukaryotes-using-whole-genome-sequence-data
#14
Saumya Agrawal, Austen R D Ganley
The ribosomal RNA genes (rDNA) encode the major rRNA species of the ribosome, and thus are essential across life. These genes are highly repetitive in most eukaryotes, forming blocks of tandem repeats that form the core of nucleoli. The primary role of the rDNA in encoding rRNA has been long understood, but more recently the rDNA has been implicated in a number of other important biological phenomena, including genome stability, cell cycle, and epigenetic silencing. Noncoding elements, primarily located in the intergenic spacer region, appear to mediate many of these phenomena...
2016: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27549079/diagnostic-performance-of-molecular-and-conventional-methods-for-identification-of-dermatophyte-species-from-clinically-infected-arabian-horses-in-egypt
#15
Yasmine H Tartor, Hend M El Damaty, Yasser S Mahmmod
BACKGROUND: Rapid and accurate identification of dermatophytes is crucial for the effective control of disease outbreaks. Current methods based on culture and microscopic characteristics may require weeks before positive identification is made. OBJECTIVES: To (i) identify the most common pathogenic dermatophytes affecting Arabian horses; (ii) compare the performance of direct microscopy (DM), culture, PCR using hair samples (PCRhair ) and PCR based on culture isolates (PCRculture ) for the diagnosis of dermatophytosis...
October 2016: Veterinary Dermatology
https://www.readbyqxmd.com/read/27543032/species-specific-optical-genosensors-for-the-detection-of-mycotoxigenic-fusarium-fungi-in-food-samples
#16
Riikka Peltomaa, Silvia Vaghini, Belén Patiño, Elena Benito-Peña, María C Moreno-Bondi
Plant-pathogenic Fusarium species, Fusarium verticillioides and Fusarium proliferatum, are the major producers of fumonisins which are one of the most common mycotoxins found in maize. Herein, we report the development of specific and sensitive genosensors for detecting these two closely related Fusarium species in food samples. The sensors are based on species-specific capture and detection probes, which bind to the intergenic spacer region of rDNA (IGS). Oligonucleotide functionalized magnetic microbeads are used to capture the target DNA which is then detected using biotinylated detection probes and a streptavidin-coupled label...
September 7, 2016: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27515974/porphyromonas-loveana-sp-nov-isolated-from-the-oral-cavity-of-australian-marsupials
#17
Philip S Bird, Darren J Trott, Deirdre Mikkelsen, Gabriel J Milinovich, Kristine M Hillman, Paul C Burrell, Linda L Blackall
An obligatory anaerobic, Gram-stain-negative coccobacillus with black-pigmented colonies was isolated from the oral cavity of selected Australian marsupial species. Phenotypic and molecular criteria showed that this bacterium was a distinct species within the genus Porphyromonas, and was closely related to Porphyromonas gingivalis and Porphyromonas gulae. This putative novel species and P. gulae could be differentiated from P. gingivalis by catalase activity. Further characterization by multi-locus enzyme electrophoresis of glutamate dehydrogenase and malate dehydrogenase enzyme mobility and matrix-assisted laser desorption ionization time-of-flight MS showed that this putative novel species could be differentiated phenotypically from P...
October 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27515668/infection-rates-of-wolbachia-sp-and-bartonella-sp-in-different-populations-of-fleas
#18
Antonio Zurita, Sara García Gutiérrez, Cristina Cutillas
In the present study, a molecular detection of Bartonella sp. and Wolbachia sp. in Ctenocephalides felis (Siphonaptera: Pulicidae) isolated from Canis lupus familiaris from different geographical areas of Spain, Iran and South Africa, and in Stenoponia tripectinata tripectinata isolated from Mus musculus from the Canary Islands has been carried out by amplification of the 16S ribosomal RNA partial gene of Wolbachia sp. and intergenic spacer region (its region) of Bartonella sp. A total of 70 % of C. felis analysed were infected by W...
November 2016: Current Microbiology
https://www.readbyqxmd.com/read/27513342/allopolyploid-origin-of-chenopodium-album-s-str-chenopodiaceae-a-molecular-and-cytogenetic-insight
#19
Karol Krak, Petr Vít, Alexander Belyayev, Jan Douda, Lucia Hreusová, Bohumil Mandák
Reticulate evolution is characterized by occasional hybridization between two species, creating a network of closely related taxa below and at the species level. In the present research, we aimed to verify the hypothesis of the allopolyploid origin of hexaploid C. album s. str., identify its putative parents and estimate the frequency of allopolyploidization events. We sampled 122 individuals of the C. album aggregate, covering most of its distribution range in Eurasia. Our samples included putative progenitors of C...
2016: PloS One
https://www.readbyqxmd.com/read/27510894/an-unusual-case-of-white-piedra-due-to-trichosporon-inkin-mimicking-trichobacteriosis
#20
Kaiwen Zhuang, Xin Ran, Yaling Dai, Jiaoqing Tang, Qin Yang, Sushmita Pradhan, Yuping Ran
White piedra is a superficial mycosis characterized by soft, white-to-tan, irregular nodules attached to the hair shafts. A 36-year-old man presented with small lumps in his pubic hair, without any other symptoms. The clinical features were suggestive of trichobacteriosis. Pathology analysis of the infected hair revealed that the concretions surrounding the hair shaft were full of fungal elements, parts of which had invaded into the cuticle. Culture on Sabouraud dextrose agar grew creamy, yellow-white colonies identified as Trichosporon inkin by the sequence of the nuclear ribosomal intergenic spacer region...
August 10, 2016: Mycopathologia
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