keyword
MENU ▼
Read by QxMD icon Read
search

barcoding

keyword
https://www.readbyqxmd.com/read/29149735/carbamazepine-lamotrigine-levetiracetam-and-valproic-acid-in-dried-blood-spots-with-liquid-chromatography-tandem-mass-spectrometry-method-development-and-validation
#1
Camilla Linder, Anna Hansson, Sara Sadek, Lars L Gustafsson, Anton Pohanka
Monitoring of antiepileptic drugs in children with epilepsy require multiple visits at a clinic for blood collection. Dried blood spot sampling is an alternative way of collection, performed at home by self-collection and can save time and costs for patients and family members. The aim was to develop and validate an LC-MS/MS dried blood spot method for carbamazepine, lamotrigine, levetiracetam and valproic acid with the requirements of using standard equipment and material in a routine laboratory setting. Whatman-903 filter paper was utilized, and discs were punched into a 96 well plate with an automated puncher and barcode reading...
November 4, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29144208/high-throughput-sequencing-of-kdna-amplicons-for-the-analysis-of-leishmania-minicircles-and-identification-of-neotropical-species
#2
Arthur Kocher, Sophie Valière, Anne-Laure Bañuls, Jérôme Murienne
Leishmania kinetoplast DNA contains thousands of small circular molecules referred to as kinetoplast DNA (kDNA) minicercles. kDNA minicircles are the preferred targets for sensitive Leishmania detection, because they are present in high copy number and contain conserved sequence blocks in which polymerase chain reaction (PCR) primers can be designed. On the other hand, the heterogenic nature of minicircle networks has hampered the use of this peculiar genomic region for strain typing. The characterization of Leishmania minicirculomes used to require isolation and cloning steps prior to sequencing...
November 16, 2017: Parasitology
https://www.readbyqxmd.com/read/29143601/morphological-description-and-dna-barcoding-study-of-sand-rice-agriophyllum-squarrosum-chenopodiaceae-collected-in-kazakhstan
#3
Yuliya Genievskaya, Saule Abugalieva, Aibatsha Zhubanysheva, Yerlan Turuspekov
BACKGROUND: Sand rice (Agriophyllum squarrosum (L.) Moq.) is an annual shrub-like plant adapted to the mobile sand dunes in desert and semi-desert regions of Asia. It has a balanced nutrient composition with relatively high concentration of lipids and proteins, which results in its nutrition being similar to legumes. Sand rice's proteins contain the full range of essential amino acids. However, calories content is more similar to wheat. These features together with desert stress resistance make sand rice a potential food crop resilient to ongoing climate change...
November 14, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/29142319/dna-metabarcoding-for-high-throughput-monitoring-of-estuarine-macrobenthic-communities
#4
Jorge Lobo, Shadi Shokralla, Maria Helena Costa, Mehrdad Hajibabaei, Filipe Oliveira Costa
Morphology-based profiling of benthic communities has been extensively applied to aquatic ecosystems' health assessment. However, it remains a low-throughput, and sometimes ambiguous, procedure. Despite DNA metabarcoding has been applied to marine benthos, a comprehensive approach providing species-level identifications for estuarine macrobenthos is still lacking. Here we report a combination of experimental and field studies to assess the aptitude of COI metabarcoding to provide robust species-level identifications for high-throughput monitoring of estuarine macrobenthos...
November 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29141868/geographic-clonal-tracking-in-macaques-provides-insights-into-hspc-migration-and-differentiation
#5
Chuanfeng Wu, Diego A Espinoza, Samson J Koelle, E Lake Potter, Rong Lu, Brian Li, Di Yang, Xing Fan, Robert E Donahue, Mario Roederer, Cynthia E Dunbar
The geographic distribution of hematopoiesis at a clonal level is of interest in understanding how hematopoietic stem and progenitor cells (HSPCs) and their progeny interact with bone marrow (BM) niches during regeneration. We tagged rhesus macaque autologous HSPCs with genetic barcodes, allowing clonal tracking over time and space after transplantation. We found marked geographic segregation of CD34(+) HSPCs for at least 6 mo posttransplantation, followed by very gradual clonal mixing at different BM sites over subsequent months to years...
November 15, 2017: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/29139259/-progress-in-molecular-identification-of-snake-drugs
#6
REVIEW
Yi Xu, Yan Lu, Hai-Ping Wu, Ying Bu
Snake drugs have high values in clinical medication for anti-inflammatory, analgesia activities and dredging collaterals. However, owing to their deficient resource and substantial profit, many counterfeits for snake drugs have appeared on the market. Traditional methods for Chinese medicine authentication include identification of origin, morphology identification, microscopy and physiochemical identification. But these methods are restricted in application because of their high morphological requirement for specimens, complex process for assays and indeterminate results guided by subjective...
August 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29137957/phylogenetic-analyses-of-transcriptome-data-resolve-familial-assignments-for-genera-of-the-red-algal-acrochaetiales-palmariales-complex-nemaliophycidae
#7
Gary W Saunders, Chris Jackson, Eric D Salomaki
Phylogenetic analyses of transcriptome data for representatives of the red algal Acrochaetiales-Palmariales Complex provided robust support for the assignment of genera to the constituent families. In the Acrochaetiales, the genera Acrochaetium, Grania, and an unnamed genus-level lineage (Acrochaetiac sp._1Aus) were assigned to the Acrochaetiaceae, while Audouinella is placed in a resurrected Audouinellaceae and Rhodochorton and Rhododrewia constitute the resurrected Rhodochortonaceae. For the Palmariales, transcriptome data solidly support the inclusion of Camontagnea and Rhodothamniella in the Rhodothamniellaceae, Meiodiscus and Rubrointrusa in the Meiodiscaceae, Rhodonematella and Rhodophysema in the Rhodophysemataceae, while Devaleraea and Palmaria remained in the Palmariaceae...
November 11, 2017: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/29134141/metabarcoding-analysis-of-the-stomach-contents-of-the-antarctic-toothfish-dissostichus-mawsoni-collected-in-the-antarctic-ocean
#8
Tae-Ho Yoon, Hye-Eun Kang, Soo Rin Lee, Jae-Bong Lee, Gun Wook Baeck, Hyun Park, Hyun-Woo Kim
Stomach contents of the Antarctic toothfish, Dissostichus mawsoni, collected from subareas 58.4 and 88.3, were analyzed using next generation sequencing (NGS) technology. After processing the raw reads generated by the MiSeq platform, a total of 131,233 contigs (130 operational taxonomic units [OTUs]) were obtained from 163 individuals in subarea 58.4, and 75,961 contigs (105 OTUs) from 164 fish in subarea 88.3. At 98% sequence identity, species names were assigned to most OTUs in this study, indicating the quality of the DNA barcode database for the Antarctic Ocean was sufficient for molecular analysis, especially for fish species...
2017: PeerJ
https://www.readbyqxmd.com/read/29134041/a-streamlined-collecting-and-preparation-protocol-for-dna-barcoding-of-lepidoptera-as-part-of-large-scale-rapid-biodiversity-assessment-projects-exemplified-by-the-indonesian-biodiversity-discovery-and-information-system-indobiosys
#9
Olga Schmidt, Axel Hausmann, Bruno Cancian de Araujo, Hari Sutrisno, Djunijanti Peggie, Stefan Schmidt
Here we present a general collecting and preparation protocol for DNA barcoding of Lepidoptera as part of large-scale rapid biodiversity assessment projects, and a comparison with alternative preserving and vouchering methods. About 98% of the sequenced specimens processed using the present collecting and preparation protocol yielded sequences with more than 500 base pairs. The study is based on the first outcomes of the Indonesian Biodiversity Discovery and Information System (IndoBioSys). IndoBioSys is a German-Indonesian research project that is conducted by the Museum für Naturkunde in Berlin and the Zoologische Staatssammlung München, in close cooperation with the Research Center for Biology - Indonesian Institute of Sciences (RCB-LIPI, Bogor)...
2017: Biodiversity Data Journal
https://www.readbyqxmd.com/read/29134040/revision-of-the-west-palaearctic-polistes-latreille-with-the-descriptions-of-two-species-an-integrative-approach-using-morphology-and-dna-barcodes-hymenoptera-vespidae
#10
Christian Schmid-Egger, Kees van Achterberg, Rainer Neumeyer, Jérôme Morinière, Stefan Schmidt
The genus Polistes is revised for the West Palaearctic region based on morphology and DNA barcodes. The revision includes all known West Palaearctic species, raising the number of species in Europe to 14 and to 17 for the West Palaearctic realm. DNA barcodes were recovered from 15 species, 14 of which belong to the subgenus Polistes, and one, P. wattii, to the subgenus Gyrostoma. An integrative taxonomic approach combining morphology and molecular data (DNA barcoding) was employed to resolve longstanding taxonomic problems in this group...
2017: ZooKeys
https://www.readbyqxmd.com/read/29134033/a-new-cryptic-lactura-from-texas-lepidoptera-zygaenoidea-lacturidae
#11
Tanner Matson, David L Wagner
A new species of Lactura is described from Texas: Lactura rubritegulasp. n. Identity of the new species can be reliably determined by both larval and adult characters, CO1 haplotypes, and its late-spring period of flight activity. Male genitalic features overlap with those of L. basistriga (Barnes & McDunnough, 1913), whereas female structures differ markedly between the pair. The new Sideroxylon-feeding species, rare in collections, is found principally in limestone areas in the vicinity San Antonio, Texas, westward through the southern Hill Country...
2017: ZooKeys
https://www.readbyqxmd.com/read/29133989/a-new-species-of-wasp-mimicking-clearwing-moth-from-peninsular-malaysia-with-dna-barcode-and-behavioural-notes-lepidoptera-sesiidae
#12
Marta Agnieszka Skowron Volponi, Paolo Volponi
A new species of clearwing moth, Pyrophleps ellawi Skowron Volponi, sp. n., is described from Peninsular Malaysia. Information on the habitat, time and conditions of occurrence, flight and mud-puddling behaviour, functional morphology, and DNA barcode are also provided. Photographs and a supplementary video from the wild demonstrate the postures and behaviour of this species of Pyrophleps, whose remaining members were described only on the basis of pinned specimens. This is the first record of this genus in Peninsular Malaysia...
2017: ZooKeys
https://www.readbyqxmd.com/read/29131846/how-many-species-and-under-what-names-using-dna-barcoding-and-genbank-data-for-west-central-african-amphibian-conservation
#13
Jessica L Deichmann, Daniel G Mulcahy, Hadrien Vanthomme, Elie Tobi, Addison H Wynn, Breda M Zimkus, Roy W McDiarmid
Development projects in west Central Africa are proceeding at an unprecedented rate, often with little concern for their effects on biodiversity. In an attempt to better understand potential impacts of a road development project on the anuran amphibian community, we conducted a biodiversity assessment employing multiple methodologies (visual encounter transects, auditory surveys, leaf litter plots and pitfall traps) to inventory species prior to construction of a new road within the buffer zone of Moukalaba-Doudou National Park, Gabon...
2017: PloS One
https://www.readbyqxmd.com/read/29130757/international-barcode-of-life-focus-on-big-biodiversity-in-south-africa
#14
Sarah J Adamowicz, Peter M Hollingsworth, Sujeevan Ratnasingham, Michelle van der Bank
Participants in the 7th International Barcode of Life Conference (Kruger National Park, South Africa, 20-24 November 2017) share the latest findings in DNA barcoding research and its increasingly diversified applications. Here, we review prevailing trends synthesized from among 429 invited and contributed abstracts, which are collated in this open-access special issue of Genome. Hosted for the first time on the African continent, the 7th Conference places special emphasis on the evolutionary origins, biogeography, and conservation of African flora and fauna...
November 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/29130756/scientific-abstracts-from-the-7th-international-barcode-of-life-conference-r%C3%A3-sum%C3%A3-s-scientifiques-du-7-e-conf%C3%A3-rence-internationale-%C3%A2-barcode-of-life-%C3%A2
#15
(no author information available yet)
No abstract text is available yet for this article.
November 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/29130755/scientific-abstracts-from-the-7th-international-barcode-of-life-conference-r%C3%A3-sum%C3%A3-s-scientifiques-du-7-e-conf%C3%A3-rence-internationale-%C3%A2-barcode-of-life-%C3%A2
#16
(no author information available yet)
No abstract text is available yet for this article.
November 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/29120913/effectiveness-of-specimen-collection-technology-in-the-reduction-of-collection-turnaround-time-and-mislabeled-specimens-in-emergency-medical-surgical-critical-care-and-maternal-child-health-departments
#17
April M Saathoff, Ryan MacDonald, Erundina Krenzischek
The objective of this study was to evaluate the impact of specimen collection technology implementation featuring computerized provider order entry, positive patient identification, bedside specimen label printing, and barcode scanning on the reduction of mislabeled specimens and collection turnaround times in the emergency, medical-surgical, critical care, and maternal child health departments at a community teaching hospital. A quantitative analysis of a nonrandomized, pre-post intervention study design evaluated the statistical significance of reduction of mislabeled specimen percentages and collection turnaround times affected by the implementation of specimen collection technology...
November 8, 2017: Computers, Informatics, Nursing: CIN
https://www.readbyqxmd.com/read/29118941/prevalence-and-subtype-identification-of-blastocystis-isolated-from-humans-in-ahvaz-southwestern-iran
#18
Roya Salehi, Ali Haghighi, C Rune Stensvold, Farnaz Kheirandish, Eznelloah Azargashb, Saber Raeghi, Cobra Kohansal, Fares Bahrami
Aim: The aim of the present study was to determine the prevalence and subtype distribution of Blastocystis and its relation with demographic data and symptoms in humans referred to medical centers in Ahvaz 2014-2015. Background: Infections with intestinal parasites are one of the most important threats to human health worldwide, especially in tropical and subtropical areas. Blastocystis sp. is a common parasite of humans with a vast variety of non-human hosts. We aimed to study the prevalence and subtypes of Blastocystis sp...
2017: Gastroenterology and Hepatology From Bed to Bench
https://www.readbyqxmd.com/read/29118779/development-of-chloroplast-genomic-resources-for-oryza-species-discrimination
#19
Yun Song, Yan Chen, Jizhou Lv, Jin Xu, Shuifang Zhu, MingFu Li, Naizhong Chen
Rice is the most important crop in the world as the staple food for over half of the population. The wild species of Oryza represent an enormous gene pool for genetic improvement of rice cultivars. Accurate and rapid identification of these species is critical for effective utilization of the wild rice germplasm. In this study, we developed valuable chloroplast molecular markers by comparing the chloroplast genomes for species identification. Four chloroplast genomes of Oryza were newly sequenced on the Illumina HiSeq platform and other 14 Oryza species chloroplast genomes from Genbank were simultaneously taken into consideration for comparative analyses...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29118680/mapping-of-standards-to-facilitate-immunization-information-exchange-through-two-dimensional-bar-coding-of-vaccine-products
#20
Saira N Haque, Suzanne West, Alan O'Connor
Purpose: A two-dimensional barcode that includes both static information (vaccine identifier) and variable information (expiration date and lot number) can facilitate streamlined documentation and sharing of vaccine administration. We sought to identify how vaccine tracking information in the two-dimensional barcode is represented in systems that are used by pharmacies, providers, and public health agencies. Methods: We identified and reviewed relevant information technology standards that govern data storage and exchange for providers, pharmacy systems, billing systems, and manufacturers to identify how each system codes the vaccine identifier, expiration date, and lot number...
2017: Perspectives in Health Information Management
keyword
keyword
75802
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"