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https://www.readbyqxmd.com/read/28346457/confined-space-facilitates-g-quadruplex-formation
#1
Prakash Shrestha, Sagun Jonchhe, Tomoko Emura, Kumi Hidaka, Masayuki Endo, Hiroshi Sugiyama, Hanbin Mao
Molecular simulations suggest that the stability of a folded macromolecule increases in a confined space due to entropic effects. However, due to the interactions between the confined molecular structure and the walls of the container, clear-cut experimental evidence for this prediction is lacking. Here, using DNA origami nanocages, we show the pure effect of confined space on the property of individual human telomeric DNA G-quadruplexes. We induce targeted mechanical unfolding of the G-quadruplex while leaving the nanocage unperturbed...
March 27, 2017: Nature Nanotechnology
https://www.readbyqxmd.com/read/28344994/a-new-broad-range-plasmid-for-dna-delivery-in-eukaryotic-cells-using-lactic-acid-bacteria-in%C3%A2-vitro-and-in%C3%A2-vivo-assays
#2
Pamela Mancha-Agresti, Mariana Martins Drumond, Fillipe Luiz Rosa do Carmo, Monica Morais Santos, Janete Soares Coelho Dos Santos, Franco Venanzi, Jean-Marc Chatel, Sophie Yvette Leclercq, Vasco Azevedo
Lactococcus lactis is well documented as a promising candidate for development of novel oral live vaccines. It has been broadly engineered for heterologous expression, as well as for plasmid expression vector delivery, directly inside eukaryotic cells, for DNA vaccine, or as therapeutic vehicle. This work describes the characteristics of a new plasmid, pExu (extra chromosomal unit), for DNA delivery using L. lactis and evaluates its functionality both by in vitro and in vivo assays. This plasmid exhibits the following features: (1) a theta origin of replication and (2) an expression cassette containing a multiple cloning site and a eukaryotic promoter, the cytomegalovirus (pCMV)...
March 17, 2017: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/28344778/methodological-advances-in-imaging-intravital-axonal-transport
#3
REVIEW
James N Sleigh, Alessio Vagnoni, Alison E Twelvetrees, Giampietro Schiavo
Axonal transport is the active process whereby neurons transport cargoes such as organelles and proteins anterogradely from the cell body to the axon terminal and retrogradely in the opposite direction. Bi-directional transport in axons is absolutely essential for the functioning and survival of neurons and appears to be negatively impacted by both aging and diseases of the nervous system, such as Alzheimer's disease and amyotrophic lateral sclerosis. The movement of individual cargoes along axons has been studied in vitro in live neurons and tissue explants for a number of years; however, it is currently unclear as to whether these systems faithfully and consistently replicate the in vivo situation...
2017: F1000Research
https://www.readbyqxmd.com/read/28342429/non-parasitic-life-cycle-of-the-cattle-tick-rhipicephalus-boophilus-microplus-in-panicum-maximum-pastures-in-northern-argentina
#4
Mariano Mastropaolo, Atilio J Mangold, Alberto A Guglielmone, Santiago Nava
The aim of this work was to study the non-parasitic phase of the Rhipicephalus microplus life cycle in Panicum maximum grasses from northern Argentina, in order to provide ecological information for designing methods of tick control. Four localities were chosen as replicates. The biological parameters measured were proportion of females ovipositing, the pre-oviposition period, the proportion of egg clusters hatching, the incubation period of eggs, larval longevity, and the total non-parasitic period (time from the exposure of the female to the date of death of the last larva) (TNPP))...
March 21, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28341934/molecular-chaperone-jiv-promotes-the-rna-replication-of-classical-swine-fever-virus
#5
Kangkang Guo, Haimin Li, Xuechao Tan, Mengmeng Wu, Qizhuang Lv, Wei Liu, Yanming Zhang
The nonstructural protein 2 (NS2) of classical swine fever virus (CSFV) is a self-splicing ribozyme wherein the precursor protein NS2-3 is cleaved, and the cleavage efficiency of NS2-3 is crucial to the replication of viral RNA. However, the proteolytic activity of NS2 autoprotease may be achieved through a cellular chaperone called J-domain protein interacting with viral protein (Jiv) or its fragment Jiv90, as evidence suggests that Jiv is required for the proper functioning of the NS2 protein of bovine viral diarrhea virus...
March 24, 2017: Virus Genes
https://www.readbyqxmd.com/read/28341043/sorghum-forage-in-precision-fed-dairy-heifer-diets
#6
F Pino, A J Heinrichs
Sorghum forage is an alternative crop that is more adapted to drier conditions and more resistant than corn to drought conditions. Thus, sorghum forage maximizes water utilization. The objective of this study was to evaluate sorghum silage (SS), including digestibility and fermentation parameters, in precision-fed dairy heifers. Eight Holstein heifers (13.7±0.6mo of age and 364.8±17.64kg of body weight) fitted with rumen cannulas were used in a replicated 4×4 Latin Square design; treatments were 4 levels of forage to concentrate ratios (85:15, 75:25, 65:35, and 55:45)...
January 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28340750/elemental-tagged-immunoassay-combined-with-inductively-coupled-plasma-mass-spectrometry-for-the-detection-of-tumor-cells-using-a-lead-sulfide-nanoparticle-label
#7
Bin Yang, Yuan Zhang, Beibei Chen, Man He, Bin Hu
This work reports an efficient, specific and sensitive immunoassay protocol for detection of tumor cells by using inductively coupled plasma mass spectrometry (ICP-MS) with lead sulfide nanoparticles (PbS NPs) as the elemental tags. PbS NPs conjugated with anti-EpCAM target the HepG2 cells with high affinity and specificity. The conditions of the ICP-MS based immunoassay for the detection of HepG2 cells were carefully optimized, including the incubation time, the concentration of the labeling probe, and the elution conditions...
May 15, 2017: Talanta
https://www.readbyqxmd.com/read/28340552/strauto-automation-and-parallelization-of-structure-analysis
#8
Vikram E Chhatre, Kevin J Emerson
BACKGROUND: Population structure inference using the software STRUCTURE has become an integral part of population genetic studies covering a broad spectrum of taxa including humans. The ever-expanding size of genetic data sets poses computational challenges for this analysis. Although at least one tool currently implements parallel computing to reduce computational overload of this analysis, it does not fully automate the use of replicate STRUCTURE analysis runs required for downstream inference of optimal K...
March 24, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28339766/higher-bioavailability-of-doxycycline-in-broiler-chickens-with-a-novel-in-feed-pharmaceutical-formulation
#9
Lilia Gutiérrez, Juan Zermeño, Yazmín Alcalá, Héctor Sumano
Bioavailability of a new, long-acting (LA) pharmaceutical preparation for administering doxycycline as in-feed medication to broiler chickens was compared to the standard in-feed administration of doxycycline. A commercial poultry house harboring Ross-308 broiler chickens, weighing 450 g, was divided into 7 sections as follows: doxy-FOLA group (n = 6,000 chickens divided into 3 replicates) medicated with 10% doxycyline, long-acting pellets at a dose of 400 g of doxycycline HCl/ton of food, resulting in a calculated dose of 48 mg/kg for 5 d; doxy-ref group (n = 6,000, divided into 3 replicates) medicated as for doxy-FOLA, but using a 20% commercial preparation of doxycycline...
February 23, 2017: Poultry Science
https://www.readbyqxmd.com/read/28339715/determination-of-pre-cecal-phosphorus-digestibility-of-inorganic-phosphates-and-bone-meal-products-in-broilers
#10
J van Harn, J W Spek, C A van Vuure, M M van Krimpen
A broiler study was performed to determine the pre-cecal phosphorus (P) digestibility of 5 P sources, 3 from animal (Delfos, Calfos, and porcine bone meal) and 2 of inorganic (monocalcium phosphate [MCP] and dicalcium phosphate [DCP]) origin. Delfos is processed from bones resulting in a dicalcium phosphate product, and Calfos is processed from bones in which part of the gelatin is removed but in which the hydroxy-apatite matrix is preserved. During the first 14 d, birds were housed in floor pens bedded with wood shavings and received a commercial starter diet...
February 22, 2017: Poultry Science
https://www.readbyqxmd.com/read/28339711/interactions-of-full-fat-canola-seed-oat-hulls-as-an-insoluble-fiber-source-and-pellet-temperature-for-nutrient-utilization-and-growth-performance-of-broiler-chickens
#11
R Barekatain, R A Swick, M Toghyani, C T de Koning
The effectiveness of the addition of oat hulls (OH) as an insoluble fiber for improving nutrient digestibility and performance of birds fed diets containing full-fat canola seed (CS) was studied. A 2 × 2 × 2 factorial arrangement of treatments was used to assess the main effects of canola source (CS vs canola meal plus oil as control), OH (0 or 3%), pellet temperature (75 and 90°C) and their interactions. A total of 576 male day-old Ross 308 chickens were assigned to 8 experimental treatments, each replicated 6 times (12 birds per replicate)...
February 22, 2017: Poultry Science
https://www.readbyqxmd.com/read/28339537/stimulatory-effects-of-fibroblast-growth-factor-2-on-proliferation-and-migration-of-uterine-luminal-epithelial-cells-during-early-pregnancy%C3%A2-%C3%A2
#12
Whasun Lim, Hyocheol Bae, Fuller W Bazer, Gwonhwa Song
Fibroblast growth factor 2 (FGF2) is a mitogen that induces proliferation, differentiation, and migration of cells, as well as angiogenesis and carcinogenesis via autocrine or paracrine actions. Fibroblast growth factor 2 expression is abundant in porcine conceptuses and endometrium during the estrous cycle and peri-implantation period of pregnancy. However, its intracellular actions in uterine epithelial cells have not been reported. The results of this study indicated abundant expression of FGFR1 and FGFR2 predominantly in uterine luminal and glandular epithelia during early pregnancy and that their expression decreased with increasing parity of the sows...
December 20, 2016: Biology of Reproduction
https://www.readbyqxmd.com/read/28339526/effect-of-glass-markings-on-drinking-rate-in-social-alcohol-drinkers
#13
David M Troy, Angela S Attwood, Olivia M Maynard, Nicholas E Scott-Samuel, Matthew Hickman, Theresa M Marteau, Marcus R Munafò
The main aim of these studies was to explore the influence of volume information on glassware on the time taken to consume an alcoholic beverage. In Study 1, male and female social alcohol consumers ( n = 159) were randomised to drink 12 fl oz of either low or standard strength lager, from either a curved glass marked with yellow tape at the midpoint or an unmarked curved glass, in a between-subjects design. In Study 2, male and female social alcohol consumers ( n = 160) were randomised to drink 12 fl oz of standard strength lager from either a curved glass marked with ¼, ½ and ¾ volume points or an unmarked curved glass, in a between-subjects design...
October 20, 2016: European Journal of Public Health
https://www.readbyqxmd.com/read/28339479/mouse-newborn-cells-allow-highly-productive-mouse-cytomegalovirus-replication-constituting-a-novel-convenient-primary-cell-culture-system
#14
Vu Thuy Khanh Le-Trilling, Mirko Trilling
Mammalian cell culture is indispensable for most aspects of current biomedical research. Immortalized cell lines are very convenient, but transforming principles (e.g. oncogenic viruses or their oncogenes) can heavily influence the experimental outcome. Primary cells do not share this apparent disadvantage but are more laborious to generate. Certain viruses (e.g. mouse cytomegalovirus) do not replicate efficiently in most transformed cell lines. In the past, such viruses have been routinely propagated on primary mouse embryonic fibroblasts (MEF) established around day 17 (d17) of gestation...
2017: PloS One
https://www.readbyqxmd.com/read/28339409/practice-based-evidence-from-a-clinical-cohort-that-received-pediatric-constraint-induced-movement-therapy
#15
Stephanie C DeLuca, Mary Rebekah Trucks, Dorian A Wallace, Sharon L Ramey
PURPOSE: Constraint-Induced Movement Therapy (CIMT) is now designated a highly efficacious treatment for children with cerebral palsy, based on rigorous clinical trials. Yet virtually no evidence confirms that these moderate to large size effects can be replicated in clinical practice for a more heterogeneous clinical population. Thus there is a need to collect and report treatment outcome data based on actual clinical practice as a critical next step for implementation. METHODS: This study presents results from a prospective study conducted on a clinical cohort of 88 children, 18 months to 12 years old (M = 55 months, SD = 5 months), who received high-intensity CIMT known as ACQUIREc...
2017: Journal of Pediatric Rehabilitation Medicine
https://www.readbyqxmd.com/read/28338050/schisandrin-a-inhibits-dengue-viral-replication-via-upregulating-antiviral-interferon-responses-through-stat-signaling-pathway
#16
Jung-Sheng Yu, Yu-Hsuan Wu, Chin-Kai Tseng, Chun-Kuang Lin, Yao-Chin Hsu, Yen-Hsu Chen, Jin-Ching Lee
Dengue virus (DENV) infects 400 million people worldwide annually. Infection of more than one serotype of DENV highly corresponds to dengue hemorrhagic fever and dengue shock syndrome, which are the leading causes of high mortality. Due to lack of effective vaccines and unavailable therapies against DENV, discovery of anti-DENV agents is urgently needed. We first characterize that Schisandrin A can inhibit the replication of four serotypes of DENV in a concentration- and time-dependent manner, with an effective half-maximal effective concentration 50% (EC50) value of 28...
March 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28336572/the-computational-and-neural-basis-of-rhythmic-timing-in-medial-premotor-cortex
#17
Hugo Merchant, Bruno B Averbeck
The neural underpinnings of rhythmic behavior, including music and dance, have been studied using the synchronization-continuation task (SCT), where subjects initially tap in synchrony with an isochronous metronome and then keep tapping at a similar rate via an internal beat mechanism. Here, we provide behavioral and neural evidence that supports a resetting drift-diffusion model (DDM) during SCT. Behaviorally, we show the model replicates the linear relation between the mean and standard-deviation of the intervals produced by monkeys in SCT...
March 23, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28336521/defending-strong-tobacco-packaging-and-labelling-regulations-in-uruguay-transnational-tobacco-control-network-versus-philip-morris-international
#18
Eric Crosbie, Particia Sosa, Stanton A Glantz
OBJECTIVE: Describe the process of enacting and defending strong tobacco packaging and labelling regulations in Uruguay amid Philip Morris International's (PMI) legal threats and challenges. METHODS: Triangulated government legislation, news sources and interviews with policy-makers and health advocates in Uruguay. RESULTS: In 2008 and 2009, the Uruguayan government enacted at the time the world's largest pictorial health warning labels (80% of front and back of package) and prohibited different packaging or presentations for cigarettes sold under a given brand...
March 23, 2017: Tobacco Control
https://www.readbyqxmd.com/read/28336519/anticollagen-type-ii-antibodies-are-associated-with-an-acute-onset-rheumatoid-arthritis-phenotype-and-prognosticate-lower-degree-of-inflammation-during-5%C3%A2-years-follow-up
#19
Vivek Anand Manivel, Mohammed Mullazehi, Leonid Padyukov, Helga Westerlind, Lars Klareskog, Lars Alfredsson, Saedis Saevarsdottir, Johan Rönnelid
OBJECTIVE: Antifibrillar collagen type II (anti-CII) antibody-positive patients with rheumatoid arthritis (RA) have early but not late signs of increased inflammation and joint erosions. We wanted to replicate this in a large RA cohort, and to relate to human leukocyte antigen (HLA)-DRB1* alleles. METHODS: Anti-CII and anti-cyclic citrullinated peptide (CCP)2 were measured at baseline in 773 patients with RA from the Swedish Epidemiological Investigation in Rheumatoid Arthritis (EIRA) study with clinical follow-up data from the Swedish Rheumatology Quality Register (SRQ) registry, and 1476 with HLA-DRB1* information...
March 23, 2017: Annals of the Rheumatic Diseases
https://www.readbyqxmd.com/read/28335094/growth-and-physiological-responses-of-broiler-chickens-to-diets-containing-raw-full-fat-soybean-meal-and-supplemented-with-a-high-impact-microbial-protease
#20
Mammo Mengesha Erdaw, Shubiao Wu, Paul A Iji, Rider A Perez-Maldonado
Objective: This study was to evaluate the change and function of the pancreas, and small intestine in relation to growth performance of broilers on diets supplemented with raw soybean meal (RSBM) and protease. Samples of test ingredients and diets, after mixing and prior to being used were also assessed on contents of anti-nutritional factors. Methods: A 3 x 3 factorial study was used, with three levels of RSBM (commercial SBM was replaced by RSBM at 0, 10 or 20%) and protease (0...
March 21, 2017: Asian-Australasian Journal of Animal Sciences
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