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Time Replication

Yu Song, Shuxiang Guo, Xuanchun Yin, Linshuai Zhang, Hideyuki Hirata, Hidenori Ishihara, Takashi Tamiya
In this paper, a novel robot-assisted catheter operating system (RCOS) has been proposed as a method to reduce physical stress and X-ray exposure time to physicians during endovascular procedures. The unique design of this system allows the physician to apply conventional bedside catheterization skills (advance, retreat and rotate) to an input catheter, which is placed at the master side to control another patient catheter placed at the slave side. For this purpose, a magnetorheological (MR) fluids-based master haptic interface has been developed to measure the axial and radial motions of an input catheter, as well as to provide the haptic feedback to the physician during the operation...
June 20, 2018: Biomedical Microdevices
Balveer Singh, Pei-Yun Jenny Wu
Progression through the cell cycle is driven by the activities of the cyclin-dependent kinase (CDK) family of enzymes, which establish an ordered passage through the cell cycle phases. CDK activity is crucial for the cellular transitions from G1 to S and G2 to M, which are highly controlled to promote the faithful duplication of the genetic material and the transmission of the genome into daughter cells, respectively. While oscillations in CDK activity are essential for cell division, how its specific dynamics may shape cellular processes remains an open question...
June 20, 2018: Current Genetics
Chidiebere Akusobi, Benjamin K Chan, Elizabeth S C P Williams, John E Wertz, Paul E Turner
The emergence of antibiotic resistance has sparked interest in phage therapy, which uses virulent phages as antibacterial agents. Bacteriophage PP01 has been studied for potential bio-control of Escherichia coli O157:H7, its natural host, but in the laboratory, PP01 can be inefficient at killing this bacterium. Thus, the goal of this study was to improve the therapeutic potential of PP01 through short-term experimental evolution. Four replicate populations of PP01 were serially passaged 21 times on non-evolving E...
June 20, 2018: Pharmaceuticals
Xaver Sewald
Viral pathogens have adapted to the host organism to exploit the cellular machinery for virus replication and to modulate the host cells for efficient systemic dissemination and immune evasion. Much of our knowledge of the effects that virus infections have on cells originates from in vitro imaging studies using experimental culture systems consisting of cell lines and primary cells. Recently, intravital microscopy using multi-photon excitation of fluorophores has been applied to observe virus dissemination and pathogenesis in real-time under physiological conditions in living organisms...
June 20, 2018: Viruses
Christopher J Lehiy, Lindsey M Reister-Hendricks, Mark G Ruder, D Scott McVey, Barbara S Drolet
BACKGROUND: Hematophagous Culicoides spp. biting midges are of great agricultural importance as livestock, equine, and wildlife pests and as vectors of the orbiviruses bluetongue, epizootic hemorrhagic disease and African horse sickness. To obtain a blood meal, midges deposit saliva containing allergens, proteases, and anti-hemostatic factors, into the dermis to facilitate feeding. Infected midges deposit virus along with the myriad of salivary proteins during feeding. The extreme efficiency with which midges are able to transmit orbiviruses is not clearly understood, as much is still unknown about the physiological trauma of the bite and immune responses to saliva deposited during feeding...
June 20, 2018: Parasites & Vectors
S Özlü, O Elibol, J Brake
The effects of temperature fluctuation during 7 d of storage on stage of blastoderm development, embryonic mortality, and hatchability of broiler hatching eggs were studied. Hatching eggs from 2 commercial flocks of Ross 308 broiler breeders at 27 and 50 wk of age, respectively, were randomly assigned to replicate chambers with either a constant temperature (Constant) of 18°C or a temperature that fluctuated (Fluctuated) over a 40 min period 3 times daily (0900 h, 1300 h, and 1700 h) between 18°C and 21°C...
June 19, 2018: Poultry Science
Suzanne E Decker, Kristen P Morie, Bo Malin-Mayo, Charla Nich, Kathleen M Carroll
BACKGROUND: Cognitive-behavior therapy (CBT) for substance use disorder is empirically supported, and may be associated with change in affect over time. OBJECTIVES: Understanding the role of affect in cocaine use disorder could help to improve CBT outcomes. METHODS: This secondary analysis included 140 adults with cocaine use disorder, 57.9% female, drawn from two randomized controlled studies of web-based cognitive-behavior therapy who completed the Positive and Negative Affect Scale (PANAS) before and during treatment...
June 20, 2018: American Journal on Addictions
Bukola A Onarinde, Ronald A Dixon
Vibrio parahaemolyticus is an environmental organism normally found in subtropical estuarine environments which can cause seafood-related human infections. Clinical disease is associated with diagnostic presence of tdh and/or trh virulence genes and identification of these genes in our preliminary isolates from retail shellfish prompted a year-long surveillance of isolates from a temperate estuary in the north of England. The microbial and environmental analysis of 117 samples of mussels, seawater or sediment showed the presence of V...
2018: Frontiers in Microbiology
Louis S Matza, Kristina S Boye, Jessica B Jordan, Kirsi Norrbacka, Raffaella Gentilella, Amara R Tiebout, Chantelle Browne, Marco Orsini Federici, Giovanni Biricolti, Katie D Stewart
Objectives: Several glucagon-like peptide-1 receptor agonists are administered as weekly injections for treatment of type 2 diabetes (T2D). These medications vary in their injection processes, and a recent study in the UK found that these differences had an impact on patient preference and health state utilities. The purpose of this study was to replicate the UK study in Italy to examine preferences of an Italian patient sample, while allowing for comparison between utilities in the UK and Italy...
2018: Patient Preference and Adherence
Bryan L Benson, Lucy Li, Jay T Myers, R Dixon Dorand, Umut A Gurkan, Alex Y Huang, Richard M Ransohoff
Leukocyte adhesion and extravasation are maximal near the transition from capillary to post-capillary venule, and are strongly influenced by a confluence of scale-dependent physical effects. Mimicking the scale of physiological vessels using in vitro microfluidic systems allows the capture of these effects on leukocyte adhesion assays, but imposes practical limits on reproducibility and reliable quantification. Here we present a microfluidic platform that provides multiple (54-512) technical replicates within a 15-minute sample collection time, coupled with an automated computer vision analysis pipeline that captures leukocyte adhesion probabilities as a function of shear and extensional stresses...
June 19, 2018: Scientific Reports
Anaïs S C Appelgren, Verena Saladin, Heinz Richner, Blandine Doligez, Karen D McCoy
BACKGROUND: In host-parasite systems, relative dispersal rates condition genetic novelty within populations and thus their adaptive potential. Knowledge of host and parasite dispersal rates can therefore help us to understand current interaction patterns in wild populations and why these patterns shift over time and space. For generalist parasites however, estimates of dispersal rates depend on both host range and the considered spatial scale. Here, we assess the relative contribution of these factors by studying the population genetic structure of a common avian ectoparasite, the hen flea Ceratophyllus gallinae, exploiting two hosts that are sympatric in our study population, the great tit Parus major and the collared flycatcher Ficedula albicollis...
June 19, 2018: BMC Evolutionary Biology
Lina Quteineh, Agnieszka Wójtowicz, Pierre-Yves Bochud, Severine Crettol, Frederik Vandenberghe, Jean-Pierre Venetz, Oriol Manuel, Dela Golshayan, Roger Lehmann, Nicolas J Mueller, Isabelle Binet, Christian van Delden, Jürg Steiger, Paul Mohacsi, Jean-Francois Dufour, Paola M Soccal, Zoltan Kutalik, Pedro Marques-Vidal, Peter Vollenweider, Mike Recher, Christoph Hess, Manuel Pascual, Chin B Eap
New-onset diabetes after transplantation (NODAT) is a complication following solid organ transplantation (SOT) and may be related to immune or inflammatory responses. We investigated whether single nucleotide polymorphisms (SNPs) within 158 immune- or inflammation-related genes contribute to NODAT in SOT recipients. The association between 263 SNPs and NODAT were investigated in a discovery sample of SOT recipients from the Swiss Transplant Cohort Study (STCS, n1 =696). Positive results were tested in a first STCS replication sample (n2 =489) and SNPs remaining significant after multiple test corrections were tested in a second SOT replication sample (n3 =156)...
June 19, 2018: American Journal of Transplantation
Alexandrea M Kranz, Leonard G Forgan, Gemma L Cole, John A Endler
Light environments critically impact species that rely on vision to survive and reproduce. Animal visual systems must accommodate changes in light that occur from minutes to years, yet the mechanistic basis of their response to spectral (color) changes is largely unknown. Here we used a laboratory experiment where replicate guppy populations were kept under three different light environments for up to 8-12 generations to explore possible differences in the expression levels of nine guppy opsin genes. Previous evidence for opsin expression-light environment 'tuning' has been either correlative or focused exclusively on the relationship between the light environment and opsin expression over one or two generations...
June 19, 2018: Evolution; International Journal of Organic Evolution
Iria Uhía, Miles Priestman, Graham Joyce, Nitya Krishnan, Vahid Shahrezaei, Brian D Robertson
Correct chromosomal segregation, coordinated with cell division, is crucial for bacterial survival, but despite extensive studies, the mechanisms underlying this remain incompletely understood in mycobacteria. We report a detailed investigation of the dynamic interactions between ParA and ParB partitioning proteins in Mycobacterium smegmatis using microfluidics and time-lapse fluorescence microscopy to observe both proteins simultaneously. During growth and division, ParB presents as a focused fluorescent spot that subsequently splits in two...
2018: PloS One
Kenneth S Jensen, Ricky Adams, Richard S Bennett, John Bernbaum, Peter B Jahrling, Michael R Holbrook
Nipah virus (NiV) is a highly pathogenic zoonotic paramyxovirus that can result in severe pulmonary disease and fatal encephalitis in humans and is responsible for outbreaks in Bangladesh, Malaysia, Singapore, India and possibly the Philippines. NiV has a negative-sense RNA genome that contains six genes and serves as a template for production of viral mRNA transcripts. NiV mRNA transcripts are subsequently translated into viral proteins. Traditionally, NiV quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) assays have relied on using primer sets that amplify a target (N that encodes the nucleocapsid) within the coding region of the viral gene that also amplifies viral mRNA...
2018: PloS One
Li Liwei, Zhou Yanjun, Jiang Yifeng, Gao Fei, Shan Tongling, Zhao Kuan, Zhang Yujiao, Li Lin, Tong Guangzhi
Porcine galectin-3 (GAL3) is a 29-kDa protein encoded by a single gene, LGALS3, located on chromosome 1. Here, using a yeast two-hybrid screen of a cDNA library from porcine alveolar macrophage cells (PAMs), we report for the first time that GAL3 interacts with nonstructural protein 12 (Nsp12) of the porcine reproductive and respiratory syndrome virus (PRRSV). Although extensive research has focused on porcine reproductive and respiratory syndrome (PRRS), little is known about the pathogen and host interactions involving individual nonstructural viral proteins, especially Nsp12...
June 16, 2018: Virus Research
Kirsten Lochbuehler, E Paul Wileyto, Melissa Mercincavage, Valentina Souprountchouk, Jordan Z Burdge, Kathy Z Tang, Joseph N Cappella, Andrew A Strasser
Objectives: Recent research has shown that message congruency is beneficial to recall of pictorial health warning label (PWL) content after initial exposure. Despite less attention to the text warning, smokers exposed to congruent PWLs were more likely to recall the text and the message. This study aimed to replicate these findings and to examine whether congruency also affects recall after multiple exposures over time. Methods: 320 daily smokers (39.7% female; cigarettes/day: M = 15...
June 16, 2018: Nicotine & Tobacco Research: Official Journal of the Society for Research on Nicotine and Tobacco
Liliana Bordeianou, Christy E Cauley, Ruchin Patel, Ronald Bleday, Sadiqa Mahmood, Kevin Kennedy, Khawaja F Ahmed, Deborah Yokoe, David Hooper, Marc Rubin
OBJECTIVE: Create and validate diverticulitis surgical site infection prediction scale. BACKGROUND: Surgical site infections cause significant morbidity after colorectal surgery. An infection prediction scale could target infection prevention bundles to high-risk patients. METHODS: Prospectively collected National Surgical Quality Improvement Program and electronic medical record data obtained on diverticulitis colectomy patients across a Healthcare Network-wide Colorectal Surgery Collaborative (5 hospitals)...
June 18, 2018: Annals of Surgery
Jill A Dosso, Kevin H Roberts, Alessandra DiGiacomo, Alan Kingstone
There is a growing consensus among researchers that a complete description of human attention and action should include information about how these processes are informed by social context. When we actively engage in co-action with others, there are characteristic changes in action kinematics, reaction time, search behavior, as well as other processes (see Sebanz et al., 2003; Becchio et al., 2010; Wahn et al., 2017). It is now important to identify precisely what is shared between co-actors in these joint action situations...
2018: Frontiers in Psychology
Claire Bedez, Christophe Lotz, Claire Batisse, Arnaud Vanden Broeck, Roland H Stote, Eduardo Howard, Karine Pradeau-Aubreton, Marc Ruff, Valérie Lamour
Type 2 DNA topoisomerases (Top2) are critical components of key protein complexes involved in DNA replication, chromosome condensation and segregation, as well as gene transcription. The Top2 were found to be the main targets of anticancer agents, leading to intensive efforts to understand their functional and physiological role as well as their molecular structure. Post-translational modifications have been reported to influence Top2 enzyme activities in particular those of the mammalian Top2α isoform. In this study, we identified phosphorylation, and for the first time, acetylation sites in the human Top2α isoform produced in eukaryotic expression systems...
June 18, 2018: Scientific Reports
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