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Time Replication

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https://www.readbyqxmd.com/read/29053958/structure-of-the-dnmt1-reader-module-complexed-with-a-unique-two-mono-ubiquitin-mark-on-histone-h3-reveals-the-basis-for-dna-methylation-maintenance
#1
Satoshi Ishiyama, Atsuya Nishiyama, Yasushi Saeki, Kei Moritsugu, Daichi Morimoto, Luna Yamaguchi, Naoko Arai, Rumie Matsumura, Toru Kawakami, Yuichi Mishima, Hironobu Hojo, Shintaro Shimamura, Fuyuki Ishikawa, Shoji Tajima, Keiji Tanaka, Mariko Ariyoshi, Masahiro Shirakawa, Mitsunori Ikeguchi, Akinori Kidera, Isao Suetake, Kyohei Arita, Makoto Nakanishi
The proper location and timing of Dnmt1 activation are essential for DNA methylation maintenance. We demonstrate here that Dnmt1 utilizes two-mono-ubiquitylated histone H3 as a unique ubiquitin mark for its recruitment to and activation at DNA methylation sites. The crystal structure of the replication foci targeting sequence (RFTS) of Dnmt1 in complex with H3-K18Ub/23Ub reveals striking differences to the known ubiquitin-recognition structures. The two ubiquitins are simultaneously bound to the RFTS with a combination of canonical hydrophobic and atypical hydrophilic interactions...
October 19, 2017: Molecular Cell
https://www.readbyqxmd.com/read/29053876/blood-hematological-and-biochemical-constituents-antioxidant-enzymes-immunity-and-lymphoid-organs-of-broiler-chicks-supplemented-with-propolis-bee-pollen-and-mannan-oligosaccharides-continuously-or-intermittently
#2
Y A Attia, H Al-Khalaifah, M S Ibrahim, A E Abd Al-Hamid, M A Al-Harthi, A El-Naggar
This study investigate the effect of bee pollen (BP) and/or propolis (Pro) as an alternative to Mannan oligosaccharides (Bio-MOS, a hydrolyzed yeast with natural and artificial flavors Alltech Inc) when given continuously or intermittently on antioxidant enzymes, immunity, weight and morphology of lymphoid organs of broilers. Thus, 324 unsexed one-day-old Arbor Acres broilers were randomly distributed into nine treatment groups, each replicated 6 times of 6 birds per replicate. The chicks were kept in wire cages and fed the same basal diet and were submitted to the following treatments: control without supplementation (control) or supplemented with BP at 300 mg, Pro at 300 mg, BP+Pro at 300 mg each and Bio-MOS at 0...
October 10, 2017: Poultry Science
https://www.readbyqxmd.com/read/29053872/resveratrol-alleviates-heat-stress-induced-impairment-of-intestinal-morphology-microflora-and-barrier-integrity-in-broilers
#3
C Zhang, X H Zhao, L Yang, X Y Chen, R S Jiang, S H Jin, Z Y Geng
This study was to investigate the effect of resveratrol on intestinal morphology, microfloras, and barrier integrity of broilers subjected to heat stress. Two-hundred-seventy 21-day-old Cobb male broilers were randomly allocated to 3 treatment groups, each of which included 6 replicates with 15 birds per replicate. The 3 treatment groups were as follows: the control group, in which birds were exposed to thermoneutral condition (22 ± 1°C), and the heat stress group and heat stress + resveratrol (400 mg/kg) group, in which birds were exposed to cyclic heat stress (33 ± 1°C for 10 h/d from 0800 to 1800 h and 22 ± 1°C for the remaining time...
October 10, 2017: Poultry Science
https://www.readbyqxmd.com/read/29053694/use-of-a-recombinant-mosquito-densovirus-as-a-gene-delivery-vector-for-the-functional-analysis-of-genes-in-mosquito-larvae
#4
Pei-Wen Liu, Jia-Bao Xu, Yun-Qiao Dong, Xiao-Guang Chen, Jin-Bao Gu
In vivo microinjection is the most commonly used gene transfer technique for analyzing the gene functions in individual mosquitoes. However, this method requires a more technically demanding operation and involves complicated procedures, especially when used in larvae due to their small size, relatively thin and fragile cuticle, and high mortality, which limit its application. In contrast, viral vectors for gene delivery have been developed to surmount extracellular and intracellular barriers. These systems have the advantages of easy manipulation, high gene transduction efficiency, long-term maintenance of gene expression, and the ability to produce persistent effects in vivo...
October 6, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29053527/a-paradigm-shift-from-brick-and-mortar-full-time-nursing-faculty-off-campus
#5
Marlene Beck, Holly B Bradley, Linda L Cook, Joslin B Leasca, Tammy Lampley, JoAnne Gatti-Petito
The organizational structure for the Master of Science in Nursing's online program at Sacred Heart University offers a remarkably different innovative faculty model. Full-time, doctorally prepared faculty reside in several different states and teach online but are fully integrated and immersed in all aspects of the college of nursing. This untraditional model, which has proven to be successful over time using best practices for online education, is replicable and offers an innovative option for online learning...
October 20, 2017: Nursing Education Perspectives
https://www.readbyqxmd.com/read/29052520/upregulation-of-dars2-by-hbv-promotes-hepatocarcinogenesis-through-the-mir-30e-5p-mapk-nfat5-pathway
#6
Xian Qin, Changsheng Li, Tao Guo, Jing Chen, Hai-Tao Wang, Yi-Tao Wang, Yu-Sha Xiao, Jun Li, Pengpeng Liu, Zhi-Su Liu, Quan-Yan Liu
BACKGROUND: Infection with the hepatitis B virus (HBV) is closely associated with the development of hepatocellular carcinoma (HCC). The osmoregulatory transcription factor nuclear factor of activated T-cells 5 (NFAT5) has been shown to play an important role in the development of many types of human cancers. The role of NFAT5 in HBV-associated HCC has never previously been investigated. METHODS: We compared expression profiles of NFAT5, DARS2 and miR-30e-5p in HCC samples, adjacent nontumor tissues and different hepatoma cell lines by quantitative real-time polymerase chain reaction and /or Western blot...
October 19, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/29051773/an-improved-microscale-method-for-extraction-of-phenolic-acids-from-maize
#7
Mariana Zavala-López, Silverio García-Lara
BACKGROUND: Phenolic acids are a major group of secondary metabolites widely distributed in plants. In the case of maize, the major proportion of these metabolites occurs in the edible grain and their antioxidant activities are associated with improvements in human health. However, conventional extraction of secondary metabolites is very time consuming and generates a substantial amount of solvent waste. One approach to resolve these limitations is the use of microscale approaches, which minimize the quantity of solvents required, as well as the sample amounts and processing times...
2017: Plant Methods
https://www.readbyqxmd.com/read/29051615/time-controllable-nkcc1-knockdown-replicates-reversible-hearing-loss-in-postnatal-mice
#8
Takahisa Watabe, Ming Xu, Miho Watanabe, Junichi Nabekura, Taiga Higuchi, Karin Hori, Mitsuo P Sato, Fumiaki Nin, Hiroshi Hibino, Kaoru Ogawa, Masatsugu Masuda, Kenji F Tanaka
Identification of the causal effects of specific proteins on recurrent and partially reversible hearing loss has been difficult because of the lack of an animal model that provides reversible gene knockdown. We have developed the transgenic mouse line Actin-tTS::Nkcc1 (tetO/tetO) for manipulatable expression of the cochlear K(+) circulation protein, NKCC1. Nkcc1 transcription was blocked by the binding of a tetracycline-dependent transcriptional silencer to the tetracycline operator sequences inserted upstream of the Nkcc1 translation initiation site...
October 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29050908/hand-hygiene-attitudes-and-practices-of-nurses-a-comparison-between-2007-and-2015
#9
Liz M Kingston, Barbara L Slevin, Nuala H O'Connell, Colum P Dunne
BACKGROUND: Hand hygiene reduces health care-associated infections significantly. However, international evidence suggests that practices are suboptimal. The objective of this study was to compare and contrast hand hygiene attitudes and practices and alcohol-based handrub (ABHR) use among nurses between 2007 and 2015. METHODS: In 2007, a random sample of nurses in a large teaching hospital was invited to complete a postal survey using a validated questionnaire. In 2015, the study was replicated among all nurses employed in a university hospital group, including the setting of the original study...
October 16, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/29050405/comparative-effects-of-in-ovo-versus-subcutaneous-administration-of-the-marek-s-disease-vaccine-and-pre-placement-holding-time-on-the-processing-yield-of-ross-708-broilers
#10
E D Peebles, T M Barbosa, T S Cummings, J Dickson, S K Womack, P D Gerard
Effects of the in ovo (i.o.) or subcutaneous (s.c.) method of administration (moa) of the Marek's disease (MD) vaccine and 4 or 18 h pre-placement holding time (pht) on the processing yield of male broilers through 49 d of age (doa) were investigated. Ross 708 broiler hatching eggs (3,900) were either i.o.-vaccinated at 18 d of incubation or chicks from eggs that were not i.o.-vaccinated were s.c.-vaccinated at hatch. The i.o. injections (50 μL) were delivered by a commercial multi-egg injector and s.c. injections (200 μL) were delivered by an automatic pneumatic s...
September 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/29049970/sexual-interest-and-sexual-self-control-in-men-with-self-reported-sexual-interest-in-children-a-first-eye-tracking-study
#11
Kirsten Jordan, Peter Fromberger, Isabel Müller, Martina Wernicke, Georg Stolpmann, Jürgen L Müller
Sexual child abuse is one of the most destructive events for child development. One possible approach to avert it is the preventive treatment of individuals with a sexual interest in children. The aim of the current eye tracking study was to compare people with a self-reported sexual interest in children who participate in the outpatient preventive treatment project "PsM", pedophilic forensic inpatients, and a non-pedophilic control group. Groups were compared with respect to sexual interest and attentional control in the presence of sexual stimuli, both assessed independently of self-report...
October 7, 2017: Journal of Psychiatric Research
https://www.readbyqxmd.com/read/29049654/association-of-blood-transfusion-from-female-donors-with-and-without-a-history-of-pregnancy-with-mortality-among-male-and-female-transfusion-recipients
#12
Camila Caram-Deelder, Aukje L Kreuger, Dorothea Evers, Karen M K de Vooght, Daan van de Kerkhof, Otto Visser, Nathalie C V Péquériaux, Francisca Hudig, Jaap Jan Zwaginga, Johanna G van der Bom, Rutger A Middelburg
Importance: Transfusion of red blood cells from female donors has been associated with increased mortality in male recipients. Objective: To quantify the association between red blood cell transfusion from female donors with and without a history of pregnancy and mortality of red blood cell recipients. Design, Setting, and Participants: Retrospective cohort study of first-time transfusion recipients at 6 major Dutch hospitals enrolled from May 30, 2005, to September 1, 2015; the final follow-up date was September 1, 2015...
October 17, 2017: JAMA: the Journal of the American Medical Association
https://www.readbyqxmd.com/read/29047370/the-recent-development-and-applications-of-fluidic-channels-by-3d-printing
#13
REVIEW
Yufeng Zhou
The technology of "Lab-on-a-Chip" allows the synthesis and analysis of chemicals and biological substance within a portable or handheld device. The 3D printed structures enable precise control of various geometries. The combination of these two technologies in recent years makes a significant progress. The current approaches of 3D printing, such as stereolithography, polyjet, and fused deposition modeling, are introduced. Their manufacture specifications, such as surface roughness, resolution, replication fidelity, cost, and fabrication time, are compared with each other...
October 18, 2017: Journal of Biomedical Science
https://www.readbyqxmd.com/read/29046869/staphylococcus-aureus-alpha-toxin-induces-the-formation-of-dynamic-tubules-labeled-with-lc3-within-host-cells-in-a-rab7-and-rab1b-dependent-manner
#14
María M López de Armentia, María C Gauron, María I Colombo
Staphylococcus aureus is a pathogen that causes severe infectious diseases that eventually lead to septic and toxic shock. S. aureus infection is characterized by the production of virulence factors, including enzymes and toxins. After internalization S. aureus resides in a phagosome labeled with Rab7 protein. Here, we show that S. aureus generates tubular structures marked with the small GTPases Rab1b and Rab7 and by the autophagic protein LC3 at early times post-infection. As shown by live cell imaging these tubular structures are highly dynamic, extend, branch and grow in length...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29046460/myeloid-dendritic-cells-repress-human-cytomegalovirus-gene-expression-and-spread-by-releasing-interferon-unrelated-soluble-antiviral-factors
#15
Bahram Kasmapour, Tobias Kubsch, Ulfert Rand, Britta Eiz-Vesper, Martin Messerle, Florian W R Vondran, Bettina Wiegmann, Axel Haverich, Luka Cicin-Sain
Cytomegalovirus (CMV) is a beta-herpesvirus that latently infects most adult humans worldwide and is a major cause of morbidity and mortality in immunocompromised hosts. Latent human CMV (HCMV) is believed to reside in precursors of myeloid-lineage, leukocytes and monocytes, which give raise to macrophages and dendritic cells. We report here that human monocyte derived DCs (mo-DC) suppress HCMV infection in coculture with infected fibroblasts target cells in an effector-to-target-ratio dependent manner. Intriguingly, optimal activation of mo-DC was achieved in coculture conditions, not by their direct infection with HCMV, implying that mo-DC may recognize unique molecular patterns on, or within, infected fibroblasts...
October 18, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29046457/hiv-1-infection-of-primary-cd4-t-cells-regulates-the-expression-of-specific-herv-k-hml-2-elements
#16
George R Young, Sandra N Terry, Lara Manganaro, Alvaro Cuesta-Dominguez, Gintaras Deikus, Dabeiba Bernal-Rubio, Laura Campisi, Ana Fernandez-Sesma, Robert Sebra, Viviana Simon, Lubbertus C F Mulder
Endogenous retroviruses (ERVs) occupy extensive regions of the human genome. Although many of these retroviral elements have lost their ability to replicate, those whose insertion took place more recently, such as the HML-2 group of HERV-K elements, still retain intact open reading frames and the capacity to produce certain viral RNA and/or proteins. Transcription of these ERVs is, however, tightly regulated by dedicated epigenetic control mechanisms. Nonetheless, it has been reported that some pathologic states, such as viral infections and certain cancers, coincide with ERV expression suggesting transcriptional reawakening is possible...
October 18, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29046447/a-functional-carboxy-terminal-fluorescent-protein-fusion-to-pseudorabies-virus-small-capsid-protein-vp26
#17
Ian B Hogue, Jolie Jean, Andrew D Esteves, Nikhila S Tanneti, Julian Scherer, Lynn W Enquist
Fluorescent protein fusions to herpesvirus capsids have proven to be a valuable method to study virus particle transport in living cells. Fluorescent protein fusions to the amino terminus of small capsid protein VP26 are the most widely-used method to visualize Pseudorabies Virus (PRV) and Herpes Simplex Virus (HSV) particles in living cells. However, these fusion proteins do not incorporate to full occupancy, and have modest effects on virus replication and pathogenesis. Recent cryo electron microscopy studies have revealed that herpesvirus small capsid proteins bind to capsids via their amino terminus, whereas the carboxy terminus is unstructured and may therefore better tolerate fluorescent protein fusions...
October 18, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29045827/yeast-srs2-helicase-promotes-redistribution-of-single-stranded-dna-bound-rpa-and-rad52-in-homologous-recombination-regulation
#18
Luisina De Tullio, Kyle Kaniecki, Youngho Kwon, J Brooks Crickard, Patrick Sung, Eric C Greene
Srs2 is a super-family 1 helicase that promotes genome stability by dismantling toxic DNA recombination intermediates. However, the mechanisms by which Srs2 remodels or resolves recombination intermediates remain poorly understood. Here, single-molecule imaging is used to visualize Srs2 in real time as it acts on single-stranded DNA (ssDNA) bound by protein factors that function in recombination. We demonstrate that Srs2 is highly processive and translocates rapidly (∼170 nt per second) in the 3'→5' direction along ssDNA saturated with replication protein A (RPA)...
October 17, 2017: Cell Reports
https://www.readbyqxmd.com/read/29045682/effects-of-hermetic-storage-on-adult-sitophilus-oryzae-l-coleoptera-curculionidae-acoustic-activity-patterns-and-mortality
#19
A W Njoroge, R W Mankin, B W Smith, D Baributsa, Thomas Phillips
Hermetic storage is of interest to farmers and warehouse managers as a method to control insect pests in small storage facilities. To develop improved understanding of effects of hermetic storage on insect pest activity and mortality over time, oxygen levels, acoustic signals, and observations of visual movement were recorded from replicates of 25, 50, and 100 adult Sitophilus oryzae (L.) (Coleoptera: Curculionidae) hermetically sealed in 500- and 1,000-ml glass jars. Recordings were done for 28 d; twice daily for the first 6 d and twice weekly thereafter...
October 16, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/29045445/occupancy-modeling-reveals-territory-level-effects-of-nest-boxes-on-the-presence-colonization-and-persistence-of-a-declining-raptor-in-a-fruit-growing-region
#20
Megan E Shave, Catherine A Lindell
Nest boxes for predators in agricultural regions are an easily implemented tool to improve local habitat quality with potential benefits for both conservation and agriculture. The potential for nest boxes to increase raptor populations in agricultural regions is of particular interest given their positions as top predators. This study examined the effects of cherry orchard nest boxes on the local breeding population of a declining species, the American Kestrel (Falco sparverius), in a fruit-growing region of Michigan...
2017: PloS One
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