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https://www.readbyqxmd.com/read/28334554/rapid-establishment-of-the-european-bank-for-induced-pluripotent-stem-cells-ebisc-the-hot-start-experience
#1
Paul A De Sousa, Rachel Steeg, Elisabeth Wachter, Kevin Bruce, Jason King, Marieke Hoeve, Shalinee Khadun, George McConnachie, Julie Holder, Andreas Kurtz, Stefanie Seltmann, Johannes Dewender, Sascha Reimann, Glyn Stacey, Orla O'Shea, Charlotte Chapman, Lyn Healy, Heiko Zimmermann, Bryan Bolton, Trisha Rawat, Isobel Atkin, Anna Veiga, Bernd Kuebler, Blanca Miranda Serano, Tomo Saric, Jürgen Hescheler, Oliver Brüstle, Michael Peitz, Cornelia Thiele, Niels Geijsen, Bjørn Holst, Christian Clausen, Majlinda Lako, Lyle Armstrong, Shailesh K Gupta, Alexander J Kvist, Ryan Hicks, Anna Jonebring, Gabriella Brolén, Andreas Ebneth, Alfredo Cabrera-Socorro, Patrik Foerch, Martine Geraerts, Tina C Stummann, Shawn Harmon, Carol George, Ian Streeter, Laura Clarke, Helen Parkinson, Peter W Harrison, Adam Faulconbridge, Luca Cherubin, Tony Burdett, Cesar Trigueros, Minal J Patel, Christa Lucas, Barry Hardy, Rok Predan, Joh Dokler, Maja Brajnik, Oliver Keminer, Ole Pless, Philip Gribbon, Carsten Claussen, Annette Ringwald, Beate Kreisel, Aidan Courtney, Timothy E Allsopp
A fast track "Hot Start" process was implemented to launch the European Bank for Induced Pluripotent Stem Cells (EBiSC) to provide early release of a range of established control and disease linked human induced pluripotent stem cell (hiPSC) lines. Established practice amongst consortium members was surveyed to arrive at harmonised and publically accessible Standard Operations Procedures (SOPs) for tissue procurement, bio-sample tracking, iPSC expansion, cryopreservation, qualification and distribution to the research community...
March 7, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28328544/non-toxic-fluorescent-phosphonium-probes-to-detect-mitochondrial-potential
#2
Ana Šarić, Ivo Crnolatac, Frédéric Bouillaud, Sandra Sobočanec, Ana-Matea Mikecin, Željka Mačak Šafranko, Todor Delgeorgiev, Ivo Piantanida, Tihomir Balog, Patrice X Petit
We evaluated our phosphonium-based fluorescent probes for selective staining of mitochondria. Currently used probes for monitoring mitochondrial membrane potential show varying degrees of interference with cell metabolism, photo-induced damage and probe binding. Here presented probes are characterised by highly efficient cellular uptake and specific accumulation in mitochondria. Fluorescent detection of the probes was accomplished using flow cytometry and confocal microscopy imaging of yeast and mammalian cells...
March 22, 2017: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/28237098/measurement-of-autolysosomal-ph-by-dual-wavelength-ratio-imaging
#3
A Saric, S Grinstein, S A Freeman
Cellular components sequestered by autophagosomes during macroautophagy must be degraded and their components recycled in order to maintain homeostasis. To this end cells orchestrate the fusion of autophagosomes with lysosomes, degradative organelles that are rich in hydrolases. Most of the lysosomal enzymes function optimally at low pH, and products of macromolecular catabolism are cotransported with protons across the autolysosomal membrane. These functions are facilitated by the ability of lysosomes to pump protons inward, acidifying their lumen...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28214505/positional-right-ventricular-obstruction-in-pectus-excavatum
#4
Katherine Underwood, Matthew Vorsanger, Muhamed Saric, Adam H Skolnick
Pectus excavatum is one of the most common congenital chest wall deformities. The degree of sternal depression, which may result in compression of the right heart by the chest wall, is variable. While typically asymptomatic, there are various symptoms that can result from severe pectus excavatum. We report on a patient with severe pectus excavatum leading to dynamic obstruction of the right ventricular outflow tract in the seated position.
April 15, 2017: American Journal of Cardiology
https://www.readbyqxmd.com/read/28191682/aortic-root-thrombus-complicated-by-left-main-coronary-artery-occlusion-visualized-by-3d-echocardiography-in-a-patient-with-continuous-flow-left-ventricular-assist-device
#5
Monique S Tanna, Alex Reyentovich, Leora B Balsam, John A Dodson, Alan F Vainrib, Ricardo J Benenstein, Barry P Rosenzweig, Muhamed Saric
Aortic root thrombus is an uncommon complication of continuous-flow left ventricular assist devices (LVAD). We present the case of a 71-year-old man with ischemic cardiomyopathy who underwent destination therapy HeartMate II LVAD placement. Eighteen months later, he presented with a cerebrovascular accident followed by myocardial infarction. Transesophageal echocardiography revealed an aortic root thrombus spanning the left and noncoronary cusps and obliterating the left main coronary artery. We discuss the incidence, risk factors, and management of aortic root thrombus in LVAD patients...
February 2017: Echocardiography
https://www.readbyqxmd.com/read/28089004/elder-mistreatment-training-gaps-among-dermatology-resident-physicians-and-opportunity-to-improve-care-of-a-vulnerable-population-a-cross-sectional-study
#6
Melissa Danesh, Julia Chang, Jillian Wong Millsop, Suzana Saric, Shufeng Li, Anne Lynn S Chang
No abstract text is available yet for this article.
February 2017: Journal of the American Academy of Dermatology
https://www.readbyqxmd.com/read/28036057/green-tea-and-other-tea-polyphenols-effects-on-sebum-production-and-acne-vulgaris
#7
REVIEW
Suzana Saric, Manisha Notay, Raja K Sivamani
Polyphenols are antioxidant molecules found in many foods including nuts, fruits, vegetables, chocolate, wine, and tea. Polyphenols have antimicrobial, anti-inflammatory, and antineoplastic properties. Recent studies suggest that tea polyphenols may be used for reducing sebum production in the skin and for treatment of acne vulgaris. This review examines the evidence for use of topically and orally ingested tea polyphenols against sebum production and for acne treatment and prevention. The PubMed database was searched for studies on tea polyphenols, sebum secretion, and acne vulgaris...
December 29, 2016: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/28009026/modeling-the-active-sites-of-co-promoted-mos2-particles-by-dft
#8
Manuel Šarić, Jan Rossmeisl, Poul Georg Moses
The atomic-scale structure of the Co-promoted MoS2 catalyst (CoMoS), used for hydrodesulfurization and as a potential replacement for platinum in the acidic hydrogen evolution reaction has been analyzed by modeling its sites using density functional theory and applying thermochemical corrections to account for different reaction conditions. The equilibrium structures of the edges, basal plane and corners have been found and used to obtain a picture of an ideal CoMoS nanoparticle under hydrodesulfurization and hydrogen evolution reaction conditions...
January 18, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28004091/comparison-of-elisa-hplc-fld-and-hplc-ms-ms-methods-for-determination-of-aflatoxin-m1-in-natural-contaminated-milk-samples
#9
Jovana Kos, Elizabet Janić Hajnal, Igor Jajić, Saša Krstović, Jasna Mastilović, Bojana Šarić, Pavle Jovanov
Presence of aflatoxin M1 (AFM1) in milk should be continuously controlled in order to protect the population from risks associated with its proven toxicity and carcinogenicity. During recent years, there has been an increase in demand for development of sensitive, accurate, simple and fast method which is reliable for detection of AFM1 at low concentrations found in milk samples. For that purpose, enzyme linked immunosorbent asssay (ELISA), high performance liquid chromatography with fluorescence detector (HPLC-FLD) and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) were optimized and validated in order to apply them for AFM1 analysis in naturally contaminated milk samples, and to assess the closeness of agreement between results of three different methods...
December 2016: Acta Chimica Slovenica
https://www.readbyqxmd.com/read/28000489/medical-futility-treatment-in-intensive-care-units
#10
Marko Jukić, Lenko Šarić, Ivana Prkić, Livia Puljak
OBJECTIVE: To investigate cases of potential medical futility treatment in intensive care unit (ICU). MATERIALS AND METHODS: Retrospective review of 1567 charts of patients treated during the three-year period (2012 - 2014) in the ICU of the University Hospital Centre Split, Croatia, was conducted. More detailed analysis of the deceased patients' (n=429) charts was performed to identify cases of potential medical futility treatment. There were 99 patients for which ICU treatment was questionable due to their low Glasgow coma scale (GCS) score...
November 2016: Acta Medica Academica
https://www.readbyqxmd.com/read/27931984/knowledge-about-sources-of-dietary-fibres-and-health-effects-using-a-validated-scale-a-cross-country-study
#11
R P F Guiné, J Duarte, M Ferreira, P Correia, M Leal, I Rumbak, I C Barić, D Komes, Z Satalić, M M Sarić, M Tarcea, Z Fazakas, D Jovanoska, D Vanevski, E Vittadini, N Pellegrini, V Szűcs, J Harangozó, A El-Kenawy, O El-Shenawy, E Yalçın, C Kösemeci, D Klava, E Straumite
OBJECTIVES: Dietary fibre (DF) is one of the components of diet that strongly contributes to health improvements, particularly on the gastrointestinal system. Hence, this work intended to evaluate the relations between some sociodemographic variables such as age, gender, level of education, living environment or country on the levels of knowledge about dietary fibre (KADF), its sources and its effects on human health, using a validated scale. STUDY DESIGN: The present study was a cross-sectional study...
December 2016: Public Health
https://www.readbyqxmd.com/read/27899830/the-development-of-the-croatian-competency-framework-for-pharmacists
#12
Iva Mucalo, Maja Ortner Hadžiabdić, Tihana Govorčinović, Martina Šarić, Andreia Bruno, Ian Bates
Objective. To adjust and validate the Global Competency Framework (GbCF) to be relevant for Croatian community and hospital pharmacists. Methods. A descriptive study was conducted in three steps: translation, consensus development, and validation by an expert panel and public consultation. Panel members were representatives from community pharmacies, hospital pharmacies, regulatory and professional bodies, academia, and industry. Results. The adapted framework consists of 96 behavioral statements organized in four clusters: Pharmaceutical Public Health, Pharmaceutical Care, Organization and Management, and Personal and Professional Competencies...
October 25, 2016: American Journal of Pharmaceutical Education
https://www.readbyqxmd.com/read/27896566/croatia-needs-a-gender-incongruence-diagnosis-for-prepubertal-children
#13
Nataša Jokić-Begić, Velimir Altabas, Vesna Antičević, Goran Arbanas, Dražen Begić, Srećko Budi, Miroslav Dumić, Marina Grubić, Jasenka Grujić, Nenad Jakušić, Gordana Stipančić, Davorka Šarić, Iva Žegura
No abstract text is available yet for this article.
November 28, 2016: Archives of Sexual Behavior
https://www.readbyqxmd.com/read/27879210/murine-transgenic-ips-cell-line-for-monitoring-and-selection-of-cardiomyocytes
#14
Azra Fatima, Guoxing Xu, Filomain Nguemo, Alexey Kuzmenkin, Karsten Burkert, Jürgen Hescheler, Tomo Šarić
We report here a transgenic murine induced pluripotent stem cell (iPSC) line expressing puromycin N-acetyltransferase (PAC) and enhanced green fluorescent protein (EGFP) under the control of α-myosin heavy chain promoter. This transgenic cell line reproducibly differentiates into EGFP-expressing cardiomyocytes (CMs) which can be generated at high purity with puromycin treatment and exhibit molecular and functional properties of immature heart muscle cells. This genetically modified iPSC line can be used for assessment of the utility of CMs for myocardial repair, pharmacological and toxicological applications and development of improved cardiac differentiation protocols...
September 2016: Stem Cell Research
https://www.readbyqxmd.com/read/27869216/erratum-lipid-membrane-mediated-attraction-between-curvature-inducing-objects
#15
Casper van der Wel, Afshin Vahid, Anđela Šarić, Timon Idema, Doris Heinrich, Daniela J Kraft
No abstract text is available yet for this article.
November 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27852343/pregnancy-and-bipolar-disorder-the-risk-of-recurrence-when-discontinuing-treatment-with-mood-stabilisers-a-systematic-review
#16
Erik Roj Larsen, Kristina Saric
OBJECTIVE: Bipolar disorder in pregnancy may be difficult to treat. The dilemma is whether the women should continue medication throughout pregnancy, and maybe accept a minor risk to harm their unborn child, or discontinue medication and increase the risk of recurrence, which can lead to maternal morbidity, thereby endangering themselves and their foetus. Design and methods In September 2016, three electronic search databases; PubMed, Scopus and PsycInfo, were used searching for clinical trials concerning this question...
November 17, 2016: Acta Neuropsychiatrica
https://www.readbyqxmd.com/read/27822161/hinari-access-to-research-in-health-program-networks-to-sustain-and-expand-success
#17
Karin Saric
No abstract text is available yet for this article.
October 2016: Journal of the Medical Library Association: JMLA
https://www.readbyqxmd.com/read/27722701/melting-transition-in-lipid-vesicles-functionalised-by-mobile-dna-linkers
#18
Stephan Jan Bachmann, Jurij Kotar, Lucia Parolini, Anđela Šarić, Pietro Cicuta, Lorenzo Di Michele, Bortolo Matteo Mognetti
We study phase behaviour of lipid-bilayer vesicles functionalised by ligand-receptor complexes made of synthetic DNA by introducing a modelling framework and a dedicated experimental platform. In particular, we perform Monte Carlo simulations that combine a coarse grained description of the lipid bilayer with state of art analytical models for multivalent ligand-receptor interactions. Using density of state calculations, we derive the partition function in pairs of vesicles and compute the number of ligand-receptor bonds as a function of temperature...
September 20, 2016: Soft Matter
https://www.readbyqxmd.com/read/27618764/lipid-membrane-mediated-attraction-between-curvature-inducing-objects
#19
Casper van der Wel, Afshin Vahid, Anđela Šarić, Timon Idema, Doris Heinrich, Daniela J Kraft
The interplay of membrane proteins is vital for many biological processes, such as cellular transport, cell division, and signal transduction between nerve cells. Theoretical considerations have led to the idea that the membrane itself mediates protein self-organization in these processes through minimization of membrane curvature energy. Here, we present a combined experimental and numerical study in which we quantify these interactions directly for the first time. In our experimental model system we control the deformation of a lipid membrane by adhering colloidal particles...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27618035/polyphenols-and-sunburn
#20
REVIEW
Suzana Saric, Raja K Sivamani
Polyphenols are antioxidant molecules found in many foods such as green tea, chocolate, grape seeds, and wine. Polyphenols have antioxidant, anti-inflammatory, and antineoplastic properties. Growing evidence suggests that polyphenols may be used for the prevention of sunburns as polyphenols decrease the damaging effects of ultraviolet A (UVA) and ultraviolet B (UVB) radiation on the skin. This review was conducted to examine the evidence for use of topically and orally ingested polyphenols in prevention of sunburns...
September 9, 2016: International Journal of Molecular Sciences
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