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https://www.readbyqxmd.com/read/27908996/draft-genome-sequence-of-aeromonas-caviae-ch129-a-marine-derived-bacterium-isolated-from-the-coast-of-s%C3%A3-o-paulo-state-brazil
#1
Flávio Augusto Cardozo, Nadia Catalina Alfonso Vargas, Cristina Kraemer Zimpel, Adalberto Pessoa, Irma Nelly Gutierrez Rivera
We report here the draft genome sequence of Aeromonas caviae CH129, a marine-derived bacterium isolated from the coast of São Paulo state, Brazil. Genomic analysis revealed genes encoding enzymes involved in binding, transport, and chitin metabolism and different virulence-associated factors.
December 1, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27907048/chemical-defence-in-a-millipede-evaluation-and-characterization-of-antimicrobial-activity-of-the-defensive-secretion-from-pachyiulus-hungaricus-karsch-1881-diplopoda-julida-julidae
#2
Slaviša Stanković, Ivica Dimkić, Ljubodrag Vujisić, Sofija Pavković-Lučić, Zvezdana Jovanović, Tatjana Stević, Ivana Sofrenić, Bojan Mitić, Vladimir Tomić
The chemical defence of the millipede Pachyiulus hungaricus is reported in the present paper, in which a chemical characterization is given and antimicrobial activity is determined. In total, independently of sex, 44 compounds were identified. All compounds belong to two groups: quinones and pentyl and hexyl esters of long-chain fatty acids. The relative abundances of quinones and non-quinones were 94.7% vs. 5.3% (males) and 87.3% vs. 12.7% (females), respectively. The two dominant quinones in both sexes were 2-methyl-1,4,-benzoquinone and 2-methoxy-3-methyl-1,4-benzoquinone...
2016: PloS One
https://www.readbyqxmd.com/read/27903452/acute-stress-induced-the-hemolytic-activity-of-the-complement-system-%C3%AE-glucan-induced-cortisol-levels-improve-the-early-immune-response-in-matrinx%C3%A3-brycon-amazonicus
#3
Luz N Franco Montoya, Talísia P Martins, Rodrigo Y Gimbo, Fábio S Zanuzzo, Elisabeth C Urbinati
This study investigated the role of endogenous cortisol on the innate immune response in matrinxã (Brycon amazonicus) fed with β-glucan, prior to and after stressor exposure and bacterial challenge. For this, we evaluated the serum cortisol and plasma glucose levels, the serum lysozyme levels, the hemolytic activity of the complement system, and the respiratory activity of leukocytes, as well as the number of circulating erythrocytes and leukocytes of fish fed during 15 days with diets containing β-glucan 0...
November 26, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27899335/design-and-characterization-of-plasmids-encoding-antigenic-peptides-of-aha1-from-aeromonas-hydrophila-as-prospective-fish-vaccines
#4
Pradipta R Rauta, Bismita Nayak, Gabriel A Monteiro, Marília Mateus
The current investigation aimed at designing DNA vaccines against Aeromonas hydrophila infections. The DNA vaccine candidates were designed to express two antigenic outer membrane protein (Aha1) peptides and to be delivered by a nanoparticle-based delivery system. Gene sequences of conserved regions of antigenic Aha1 [aha1(211-381), aha1(211-381)opt, aha1(703-999) and aha1(703-999)opt] were cloned into pVAX-GFP expression vector. The selected DNA vaccine candidates were purified from E. coli DH5α and transfected into Chinese hamster ovary cells...
November 26, 2016: Journal of Biotechnology
https://www.readbyqxmd.com/read/27898025/identification-and-characterization-of-micrornas-in-the-liver-of-blunt-snout-bream-megalobrama-amblycephala-infected-by-aeromonas-hydrophila
#5
Lei Cui, Hongtao Hu, Wei Wei, Weimin Wang, Hong Liu
MicroRNAs (miRNAs) are small RNA molecules that play key roles in regulation of various biological processes. In order to better understand the biological significance of miRNAs in the context of Aeromonas hydrophila infection in Megalobrama amblycephala, small RNA libraries obtained from fish liver at 0 (non-infection), 4, and 24 h post infection (poi) were sequenced using Illumina deep sequencing technology. A total of 11,244,207, 9,212,958, and 7,939,157 clean reads were obtained from these three RNA libraries, respectively...
November 25, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27891182/case-control-study-of-diarrheal-disease-etiology-in-individuals-over-5%C3%A2-years-in-southwest-china
#6
Shun-Xian Zhang, Chun-Li Yang, Wen-Peng Gu, Lin Ai, Emmanuel Serrano, Pin Yang, Xia Zhou, Shi-Zhu Li, Shan Lv, Zhi-Sheng Dang, Jun-Hu Chen, Wei Hu, Li-Guang Tian, Jia-Xu Chen, Xiao-Nong Zhou
BACKGROUND: Acute diarrhea is one of the major public health problems worldwide. Most of studies on acute diarrhea have been made on infants aged below 5 years and few efforts have been made to identify the etiological agents of acute diarrhea in people over five, especially in China. METHODS: 271 diarrhea cases and 149 healthy controls over 5 years were recruited from four participating hospitals between June 2014 and July 2015. Each stool specimen was collected to detect a series of enteric pathogens, involving five viruses (Rotavirus group A, RVA; Norovirus, NoV; Sapovirus, SaV; Astrovirus, As; and Adenovirus, Ad), seven bacteria (diarrheagenic Escherichia coli, DEC; non-typhoidal Salmonella, NTS; Shigella spp...
2016: Gut Pathogens
https://www.readbyqxmd.com/read/27889136/characterization-of-the-microbiota-in-lightly-salted-bighead-carp-aristichthys-nobilis-fillets-stored-at-4%C3%A2-%C3%A2-c
#7
Xiaochang Liu, Yuemei Zhang, Dapeng Li, Yongkang Luo
The microbiota of unsalted and salted (dry-cured with 2% salt) bighead carp (Aristichthys nobilis) fillets during storage at 4 °C were identified by 16S rRNA gene analysis. Eleven genera were present in the initial microbiota of bighead carp fillets, where Acinetobacter, Aeromonas and Kocuria were the dominant bacteria. As storage time progressed, the microbial composition of both unsalted and salted fillets became less diverse. Additionally, differences in microbiota were observed between these two treatments...
April 2017: Food Microbiology
https://www.readbyqxmd.com/read/27888132/vitamin-e-deficiency-depressed-fish-growth-disease-resistance-and-the-immunity-and-structural-integrity-of-immune-organs-in-grass-carp-ctenopharyngodon-idella-referring-to-nf-%C3%AE%C2%BAb-tor-and-nrf2-signaling
#8
Jia-Hong Pan, Lin Feng, Wei-Dan Jiang, Pei Wu, Sheng-Yao Kuang, Ling Tang, Yong-An Zhang, Xiao-Qiu Zhou, Yang Liu
This study investigated the effects of dietary vitamin E on growth, disease resistance and the immunity and structural integrity of head kidney, spleen and skin in grass carp (Ctenopharyngodon idella). The fish were fed six diets containing graded levels of vitamin E (0, 45, 90, 135, 180 and 225 mg/kg diet) for 10 weeks. Subsequently, a challenge test was conducted by injection of Aeromonas hydrophila. The results showed that compared with optimal vitamin E supplementation, vitamin E deficiency caused depressed growth, poor survival rates and increased skin lesion morbidity in grass carp...
November 22, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27885558/aeromonas-and-plesiomonas-species-from-scarlet-ibis-eudocimus-ruber-and-their-environment-monitoring-antimicrobial-susceptibility-and-virulence
#9
Débora Souza Collares Maia Castelo-Branco, Aline Lobão da Silva, Frederico Ozanan Barros Monteiro, Glaucia Morgana de Melo Guedes, Jamille Alencar Sales, Jonathas Sales de Oliveira, José Erisvaldo Maia Junior, Stefânia Araújo Miranda, José Júlio Costa Sidrim, Lucas Pereira de Alencar, Raimunda Sâmia Nogueira Brilhante, Rossana de Aguiar Cordeiro, Tereza de Jesus Pinheiro Gomes Bandeira, Waldemiro de Aquino Pereira Neto, Marcos Fábio Gadelha Rocha
The present study aimed at evaluating the role of captive scarlet ibises (Eudocimus ruber) and their environment as reservoirs of Aeromonas spp. and Plesiomonas spp., and analyzing the in vitro antimicrobial susceptibility and virulence of the recovered bacterial isolates. Thus, non-lactose and weak-lactose fermenting, oxidase positive Gram-negative bacilli were recovered from cloacal samples (n = 30) of scarlet ibises kept in a conservational facility and from water samples (n = 30) from their environment...
November 24, 2016: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/27881349/-diagnosis-of-drowning-by-detecting-gyrb-and-16s-rrna-genes-of-aeromonas-hydrophila-using-pcr-capillary-electrophoresis
#10
Bai-Sheng Mai, Qu-Yi Xu, Chao Liu, Jian Zhao, Ya-Li Han
OBJECTIVE: To establish a method for diagnosis of freshwater drowning by amplifying gyrB and 16S rRNA genes of Aeromonas hydrophila using PCR coupled with capillary electrophoresis (CE). METHODS: DNA samples were extracted from human, 18 planktons (including Candida albicans, Aeromonas hydrophila, and 16 species of algae), and 30 cases of tissue samples (including the lung, liver, and kidney, all examined with microwave digestion-vacuum filtration-automated scanning electron microscopy) from human cadavers, including 28 freshwater drowning victims and 2 with natural death...
November 20, 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/27876624/differences-in-structure-and-changes-in-gene-regulation-of-murrel-molecular-chaperone-hsp-family-during-epizootic-ulcerative-syndrome-eus-infection
#11
Akila Sathyamoorthy, Mukesh Kumar Chaurasia, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Ramasamy Harikrishnan, Jesu Arockiaraj
Heat shock proteins (HSPs) are immunogenic, ubiquitous class of molecular chaperones, which are induced in response to various environmental and microbial stressful conditions. It plays a vital role in maintaining cellular protein homeostasis in eukaryotic cells. In this study, we described a comprehensive comparative data by bioinformatics approach on three different full length cDNA sequences of HSP family at molecular level. The cDNA sequences of three HSPs were identified from constructed cDNA library of Channa striatus and named as CsCPN60, CsHSP60 and CsHSP70...
November 20, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27867114/serine-protease-inhibitor-kazal-type-2-is-expressed-in-the-male-reproductive-tract-of-carp-with-a-possible-role-in-antimicrobial-protection
#12
Mariola A Dietrich, Mariola Słowińska, Halina Karol, Mikołaj Adamek, Dieter Steinhagen, Anna Hejmej, Barbara Bilińska, Andrzej Ciereszko
The presence of the low-molecular-mass serine protease inhibitor Kazal-type (Spink) is a characteristic feature of vertebrate semen. Its main function is control of the serine protease in the acrosome, acrosin. Here we showed for the first time that Spink is present in the seminal plasma of carp, which have anacrosomal spermatozoa. Using a three-step isolation procedure that consisted in gel filtration and RP-HPLC and re-RP-HPLC, we isolated this inhibitor and identified it as serine protease inhibitor Kazal-type 2 (Spink2), a reproductive-derived member of the Spink family...
November 17, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27858184/pathogenic-mechanisms-of-actin-cross-linking-toxins-peeling-away-the-layers
#13
Elena Kudryashova, David B Heisler, Dmitri S Kudryashov
Actin cross-linking toxins are produced by Gram-negative bacteria from Vibrio and Aeromonas genera. The toxins were named actin cross-linking domains (ACD), since the first and most of the subsequently discovered ACDs were found as effector domains in larger MARTX and VgrG toxins. Among recognized human pathogens, ACD is produced by Vibrio cholerae, Vibrio vulnificus, and Aeromonas hydrophila. Upon delivery to the cytoplasm of a host cell, ACD covalently cross-links actin monomers into non-polymerizable actin oligomers of various lengths...
November 18, 2016: Current Topics in Microbiology and Immunology
https://www.readbyqxmd.com/read/27856595/draft-genome-sequence-of-pseudomonas-mosselii-gil3-isolated-from-catfish-and-antagonistic-against-hypervirulent-aeromonas-hydrophila
#14
Dunhua Zhang, De-Hai Xu, Junqiang Qiu, Cody R Rasmussen-Ivey, Mark R Liles, Benjamin H Beck
Pseudomonas mosselii Gil3 was isolated from a catfish that survived from lethal challenge with hypervirulent Aeromonas hydrophila (vAh). When assayed in vitro, the bacterium showed antagonism against vAh. Sequence analysis revealed that the genome of P. mosselii Gil3 encodes numerous aromatic metabolism pathways and proteins for biosynthesis of antimicrobial compounds.
November 17, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27856589/draft-genome-sequence-of-marine-derived-aeromonas-caviae-chz306-a-potential-chitinase-producer-strain
#15
Flávio Augusto Cardozo, Cristina Kraemer Zimpel, Ana Marcia Sa Guimaraes, Adalberto Pessoa, Irma Nelly Gutierrez Rivera
We report here a draft genome sequence of Aeromonas caviae CHZ306, a marine-derived bacterium with the ability to hydrolyze chitin and express high levels of chitinases. The assembly resulted in 65 scaffolds with approximately 4.78 Mb. Genomic analysis revealed different genes encoding chitin-degrading enzymes that can be used for chitin derivative production.
November 17, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27853553/the-role-of-three-heat-shock-protein-genes-in-the-immune-response-to-aeromonas-hydrophila-challenge-in-marbled-eel-anguilla-marmorata
#16
Fenfei Liang, Guosong Zhang, Shaowu Yin, Li Wang
Heat shock proteins (HSPs) are highly conserved molecular chaperones that play critical roles in both innate and adaptive immunity. However, little information about HSPs from marbled eel Anguilla marmorata is known. In this study, the full-length Amhsp90 (2527 bp), Amhsp70 (2443 bp) and Amhsc70 (2247 bp) were first cloned from A. marmorata, using rapid amplification of cDNA ends, containing open reading frames of 2181, 1932 and 1950 bp in length, and encoding proteins with 726, 643 and 649 amino acids, respectively...
October 2016: Royal Society Open Science
https://www.readbyqxmd.com/read/27836722/multiple-gene-and-transcript-variants-encoding-trout-c-polysaccharide-binding-proteins-are-differentially-but-strongly-induced-after-infection-with-aeromonas-salmonicida
#17
Judith M Köbis, Henrike Rebl, Tom Goldammer, Alexander Rebl
Two 'trout C-polysaccharide-binding proteins,' TCBP1 and -2, with relevance to early inflammatory events have been discovered in the last century. The present study characterises the respective cDNA sequences from rainbow trout (Oncorhynchus mykiss), including multiple TCBP1 transcript variants. These variants are generated either by the use of alternative splice sites or the exclusion of exons. The longest mRNA isoform, TCBP1-1, encodes a 245-aa protein with a large signal peptide and a complement component C1q domain...
November 8, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27832917/immune-response-characterization-and-vaccine-potential-of-a-recombinant-chimera-comprising-b-cell-epitope-of-aeromonas-hydrophila-outer-membrane-protein-c-and-ltb
#18
Mahima Sharma, Aparna Dixit
Aeromonas hydrophila is one of the most virulent fish pathogens, causing colossal economic losses to the aquaculture industry annually. The absence of a safe and effective vaccine makes it very difficult to control this infection. Outer membrane proteins have been widely illustrated to confer protective immunity against a broad spectrum of gram negative bacteria. In the current study, we have analyzed the ability of B-cell epitopes of A. hydrophila's outer membrane protein C (OmpC) to confer protection against bacterial virulence...
December 7, 2016: Vaccine
https://www.readbyqxmd.com/read/27830678/-cloning-and-expression-analysis-of-two-pro-inflammatory-cytokines-il-1%C3%AE-and-its-receptor-il-1r2-in-the-asian-swamp-eel-monopterus-albus
#19
Q Q Xu, P Xu, J W Zhou, T S Pan, R Tuo, K Ai, D Q Yang
Interleukin-1β (IL-1β) is the prototypic pro-inflammatory cytokine, whose functions are mediated through interaction with its receptors (IL-1R1 and IL-1R2). Herein, we cloned the full-length cDNA and genomic DNA of IL-1β and IL-1R2 in the Asian swamp eel (Monopterus albus). The eel IL-1β cDNA encodes a putative polypeptide of 246 amino acids. The protein sequence includes a typical IL-1 family signature, but lacked an interleukin-converting enzyme cleavage site. The genomic DNA of eel IL-1β was 2520 bp and comprised five exons and four introns...
September 2016: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/27827926/assessment-of-chemical-impact-of-invasive-bryozoan-pectinatella-magnifica-on-the-environment-cytotoxicity-and-antimicrobial-activity-of-p-magnifica-extracts
#20
Peter Kollar, Karel Šmejkal, Hana Salmonová, Eva Vlková, Olga Lepšová-Skácelová, Zuzana Balounová, Josef Rajchard, Josef Cvačka, Libor Jaša, Pavel Babica, Jiří Pazourek
Pectinatella magnifica, an invasive bryozoan, might significantly affect ecosystem balance due to its massive occurrence in many areas in Europe and other parts of the world. Biological and chemical analyses are needed to get complete information about the impact of the animal on the environment. In this paper, we aimed to evaluate in vitro cytotoxic effects of five extracts prepared from P. magnifica using LDH assay on THP-1 cell line. Antimicrobial activities of extracts against 22 different bacterial strains were tested by microdilution method...
November 4, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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