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Monika Mioduchowska, Michał Jan Czyż, Bartłomiej Gołdyn, Jarosław Kur, Jerzy Sell
The cytochrome c oxidase subunit I (cox1) gene is the main mitochondrial molecular marker playing a pivotal role in phylogenetic research and is a crucial barcode sequence. Folmer's "universal" primers designed to amplify this gene in metazoan invertebrates allowed quick and easy barcode and phylogenetic analysis. On the other hand, the increase in the number of studies on barcoding leads to more frequent publishing of incorrect sequences, due to amplification of non-target taxa, and insufficient analysis of the obtained sequences...
2018: PloS One
Zhong-Min Dai, Yanan Xiong, Weiran He, Yukun Fang, Ye-Qing Qian, Xiao-Jing Zhu
Wnt signaling play important roles in a variety of developmental and pathological processes. Here we show that Wntless, the main regulator for Wnt secretion, is involved in the innate immune response of the giant freshwater prawn, Macrobrachium rosenbergii. The full-length cDNA of the prawn Wntless (named MrWntless) is 2173 bp in length and contains a 1602-bp open reading frame (ORF), which is conceptually translated into a 533-amino acids sequence. MrWntless protein contains a highly conserved Wnt-binding domain which is required for secretion of Wnt ligands, and exhibits 57-67% identity with known Wntless proteins of other animals...
June 19, 2018: Fish & Shellfish Immunology
Yulin Bai, Yubang Shen, Xiao-Yan Xu, Yuqi Bai, Yuan Fang, Meng Zhang, Yiheng Miao, Xueshu Zhang, Jia-le Li
Growth arrest and DNA damage inducible 45-beta (Gadd45B) is essential for mitogen-activated protein kinases (MAPK) activities, and involved in regulating growth, apoptosis, and DNA demethylation. In the present study, the cDNA of gcGadd45Ba and gcGadd45Bb in grass carp was identified. And the expression levels show that they were widely distributed in the tested tissues and showed significant immune responses both in vitro and in vivo after challenge with bacteria and pathogen-associated molecular patterns (PAMPs)...
June 19, 2018: Developmental and Comparative Immunology
Claire Bernardin-Souibgui, Sylvie Barraud, Emilie Bourgeois, Jean-Baptiste Aubin, Celine Becouze-Lareure, Laure Wiest, Laurence Marjolet, Celine Colinon, Ghislain Lipeme Kouyi, Benoit Cournoyer, Didier Blaha
The nature and fate of urban contaminants washed by stormwater events and accumulating in a detention basin (DB) were investigated. Relations between bacterial and chemical contaminants of trapped urban sediments, and field parameters were analyzed. Fecal indicators and some pathogens known to be environmentally transmitted (Nocardia, Pseudomonas aeruginosa, and Aeromonas caviae) were tracked, and their persistence investigated. Six sampling campaigns were carried out over 3 years, using five sites including a settling chamber (SC)...
June 21, 2018: Environmental Science and Pollution Research International
Kateryna Fesko, Dmitry Suplatov, Vytas Švedas
Understanding the role of specific amino acid residues in the molecular mechanism of a protein's function is one of the most challenging problems in modern biology. A systematic bioinformatic analysis of protein families and superfamilies can help in the study of structure-function relationships and in the design of improved variants of enzymes/proteins, but represents a methodological challenge. The pyridoxal-5'-phosphate (PLP)-dependent enzymes are catalytically diverse and include the aspartate aminotransferase superfamily which implements a common structural framework known as type fold I...
June 2018: FEBS Open Bio
Tolga Çakmak, İbrahim Çaltekin, Emre Gökçen, Atakan Savrun, Erdoğan Yaşar
Kounis Syndrome is the development of acute coronary syndrome (ACS) because of allergic reactions; allergens, such as foods, insect venom, iodine contrast agent or drugs can cause this syndrome. Hirudotherapy (leech therapy) is increasingly used as a useful therapeutic option in a variety of medical and surgical procedures, and potential complications related to this therapy include Aeromonas species infections, bleeding, anemia and allergic reactions. Here, we present a patient diagnosed as Type 1 Kounis Syndrome after using hirudotherapy for her knee aches...
June 2018: Turkish Journal of Emergency Medicine
Eiji Kakizaki, Ai Sonoda, Masahiro Sakai, Nobuhiro Yukawa
We developed a novel molecular tool for assisting the diagnosis of death by drowning and evaluated its validity in forensic practical cases. Two novel sets of loop-mediated isothermal amplification (LAMP) primers were designed to detect either representative freshwater (Aeromonas) or marine (Vibrio, Photobacterium, Listonella) bacterioplankton (aquatic bacteria) in one tube using the LAMP technique. The assay involves only mixing template DNA with seven reagents and incubating at 64°C for 80min and does not require special or expensive equipment because detection is based on visual observation under natural light...
May 30, 2018: Forensic Science International
Rasha M Reda, M A El-Hady, Khaled M Selim, Hassanin M El-Sayed
The present study was conducted to evaluate the supplementation of three autochthonous Bacillus strains (B. subtilis, B. amyloliquefaciens and B. cereus) and a commercial B. amyloliquefaciensin doses of 1 × 1010 CFU/kg on the growth performance, hematology, antioxidant activities, digestive enzyme levels, immune status and disease resistance of Clarias gariepinus. A total of 300 fish (75.23 ± 1.6 g) were randomly divided into 5 groups (each group was subdivided into 2 subgroups, 30 fish/each). The control group was fed basal diet (D0 )...
June 16, 2018: Fish & Shellfish Immunology
Dandan Huang, Zhiyi Bai, Jiexuan Shen, Liting Zhao, Jiale Li
Tumor necrosis factor receptor-associated factor 6 (TRAF6) acts as a central intracellular signal adapter molecule that mediates the tumor necrosis factor receptor superfamily and the interleukin-1 receptor/Toll-like receptor family in vertebrates and invertebrates. In the present study, HcTRAF6, a molluscan homologue of TRAF6 from Hyriopsis cumingii, has been cloned and identified. The entire open reading frame of HcTRAF6 was found to comprise a 1965-bp region that encodes a predicted protein of 654 amino acids, which contains conserved characteristic domains including a RING domain, two TRAF-type zinc finger domains, a typical coiled coil and the MATH domain...
June 18, 2018: Fish & Shellfish Immunology
Prapansak Srisapoome, Nawanith Klongklaew, Nontawith Areechon, Ratree Wongpanya
Anti-lipopolysaccharide factor (ALF) is an immune-related protein that is crucially involved in immune defense mechanisms against invading pathogens in crustaceans. In the current study, three different ALFs of giant river prawn (Mr-ALF3, Mr-ALF8 and Mr-ALF9) were discovered. Based on sequence analysis, Mr-ALF3 and Mr-ALF9 were identified as new members of ALFs in crustaceans (groups F and G, respectively). Structurally, each newly identified Mr-ALF contained three α-helices packed against a four-stranded β-sheet bearing the LPS-binding motif, which usually binds to the cell wall components of bacteria...
June 14, 2018: Fish & Shellfish Immunology
Yan Liu, Ying-Hao Zhang, Tong Li, Xiao-Tong Cao, Yang Zhou, Jun-Fa Yuan, Ze-Mao Gu, Jiang-Feng Lan
Antimicrobial peptides (AMPs) play important roles in innate immunity against pathogens and lysozymes are a particularly type of AMP. Lysozymes are hydrolytic enzymes that are characterized by their ability to cleave the beta-(1,4)-glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in peptidoglycan, which is the major bacterial cell wall polymer. In this work, a lysozyme was identified from Procambarus clarkii and designated PcLys-i3. Quantitative RT-PCR was used to analyze the tissue distribution and expression profiles of PcLys-i3...
May 31, 2018: Developmental and Comparative Immunology
Meimei Zhang, Qingpi Yan, Leilei Mao, Suyun Wang, Lixing Huang, Xiaojin Xu, Yingxue Qin
The success of the pathogenic bacteria is partly attributable to their ability to thwart host innate immune responses, which includes resisting the antimicrobial functions of macrophages. And reactive oxygen species (ROS) is one of the most effective antimicrobial components of macrophages to kill invading bacteria. Our previous studies found that Aeromonas hydrophila can survive in fish macrophages, which suggested that this bacterium might take fish macrophages as their shelters to resist drug killings and other immune damage...
June 12, 2018: Gene
Theresa Burns, Brian R Radke, Tyler Stitt, Carl Ribble
Antimicrobial resistance (AMR) data generated by diagnostic animal health laboratories are underutilized for AMR reporting in Canada. Data assessment, review of practices in other jurisdictions, and expert interviews were used to develop an evidence-guided plan to generate AMR reports from British Columbia Animal Health Centre (AHC) data that would provide transparent, timely, and useful information to public health practitioners, the food animal sector, and the general public. Using the Canadian Animal Health Surveillance Network (CAHSN) platform was the most efficient method of data retrieval...
May 2018: Canadian Veterinary Journal. la Revue Vétérinaire Canadienne
Salih Kumru, Hasan C Tekedar, Matt J Griffin, Geoffrey C Waldbieser, Mark R Liles, Tad Sonstegard, Steven G Schroeder, Mark L Lawrence, Attila Karsi
Aeromonas bestiarum is a Gram-negative mesophilic motile bacterium causing acute hemorrhagic septicemia or chronic skin ulcers in fish. Here, we report the draft genome sequence of A. bestiarum strain GA97-22, which was isolated from rainbow trout in 1997. This genome sequence will improve our understanding of the complex taxonomy of motile aeromonads.
June 14, 2018: Genome Announcements
Shailendra Kumar Singh, Samuel R Major, Haiyuan Cai, Feng Chen, Russell T Hill, Yantao Li
We report here the whole-genome sequencing results of two bacterial isolates, Aeromonas jandaei IMET J and Cloacibacterium normanense IMET F, that inhibit (possibly due to denitrifying gene clusters) and promote (possibly due to an ammonification system), respectively, the growth of the microalgal strains Scenedesmus HTB1 and Chlorella vulgaris 1807.
June 14, 2018: Genome Announcements
Jinwei Gao, Bingwen Xi, Kai Chen, Rui Song, Ting Qin, Jun Xie, Liangkun Pan
Stress is an important contributing factor in the outbreak of infectious fish diseases. To comprehensively understand the impact of catecholamine stress hormone norepinephrine (NE) on the pathogenicity of Aeromonas hydrophila, we assessed variations in bacterial growth, virulence-related genes expression and virulence factors activity after NE addition in serum-SAPI medium. Further, we assessed the effects of NE on A. hydrophila virulence in vivo by challenging fish with pathogenic strain AH196 and following with or without NE injection...
June 13, 2018: MicrobiologyOpen
Rasha M Reda, Khaled M Selim, Rania Mahmoud, Iman E El-Araby
The present study investigated the effects of dietary supplementation of yeast nucleotides on the hematology, antioxidant activity, non-specific immunity, expression of intestinal cytokines, and disease resistance in Nile Tilapia (Oreochromis niloticus). Fish weighing 42.90 ± 0.14 g were randomly divided into four groups. Each group was set in triplicate (15 fish per replicate). Fish were fed on four dietary levels of yeast nucleotides (NTs) supplemented with the basal diet 0% (control), 0.05%, 0.15%, and 0...
June 9, 2018: Fish & Shellfish Immunology
Yingying Zhang, Hui Yang, Weijia Song, Dan Cui, Lixin Wang
Lysozymes act as important innate immunity molecule against various pathogen infections. In the present study, a new c-type and g-type lysozymes were isolated from rare minnow Gobiocypris rarus. The deduced amino acid sequence of c-type lysozyme contained 145 residues and possessed conserved catalytic residues (Glu53 and Asp72 ). The deduced g-type lysozyme contained 185 residues and possessed three conserved catalytic residues (Glu73 , Asp86 and Asp97 ). qPCR analysis revealed that GrlysG and GrlysC were constitutively expressed in all examined tissues...
June 2018: Genes & Genomics
Quanxin Gao, Yanfeng Yue, Minghua Min, Shiming Peng, Zhaohong Shi, Wenquan Sheng, Tao Zhang
Toll like receptor (TLR) 5 and 9 are important members of the TLR family that play key roles in innate immunity in all vertebrates. In this study, paTLR5 and paTLR9 were identified in silver pomfret (Pampus argenteus), a marine teleost of great economic value. Open reading frames (ORFs) of paTLR5 and paTLR9 are 2646 and 3225 bp, encoding polypeptides of 881 and 1074 amino acids, respectively. Sequence analysis revealed several conserved characteristic features, including signal peptides, leucine-rich repeat (LRR) motifs, and a Toll/interleukin-I receptor (TIR) domain...
June 8, 2018: Fish & Shellfish Immunology
Meng Chen, Mingmei Ding, Yuan Li, Xiaofang Zhong, Shuo Liu, Zheng Guo, Xiaoxue Yin, Shengli Fu, Jianmin Ye
Complement component 1q (C1q) is the initial protein of the classical complement pathway and plays an important role in immune response against bacterial infection. In this study, the full-length of C1q subcomponent A, B and C chain genes (C1qA, C1qB and C1qC) were identified and characterized from Nile tilapia (Oreochromis niloticus). Molecular characterization of these three C1q subcomponents (OnC1qs) harbored conserved amino acids through analyses of multiple sequence alignment and phylogenetic tree, which were homologous to other teleost species...
June 8, 2018: Developmental and Comparative Immunology
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