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https://www.readbyqxmd.com/read/27933080/identification-of-a-dolabellane-type-diterpene-synthase-and-other-root-expressed-diterpene-synthases-in-arabidopsis
#1
Qiang Wang, Meirong Jia, Jung-Hyun Huh, Andrew Muchlinski, Reuben J Peters, Dorothea Tholl
Arabidopsis thaliana maintains a complex metabolism for the production of secondary or specialized metabolites. Such metabolites include volatile and semivolatile terpenes, which have been associated with direct and indirect defensive activities in flowers and leaves. In comparison, the structural diversity and function of terpenes in Arabidopsis roots has remained largely unexplored despite a substantial number of root-expressed genes in the Arabidopsis terpene synthase (TPS) gene family. We show that five root-expressed TPSs of an expanded subfamily-a type clade in the Arabidopsis TPS family function as class I diterpene synthases that predominantly convert geranylgeranyl diphosphate (GGPP) to different semi-volatile diterpene products, which are in part detectable at low levels in the ecotypes Columbia (Col) and Cape Verde Island (Cvi)...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27933071/stress-mediated-cis-element-transcription-factor-interactions-interconnecting-primary-and-specialized-metabolism-in-planta
#2
REVIEW
S A Sheshadri, M J Nishanth, Bindu Simon
Plant specialized metabolites are being used worldwide as therapeutic agents against several diseases. Since the precursors for specialized metabolites come through primary metabolism, extensive investigations have been carried out to understand the detailed connection between primary and specialized metabolism at various levels. Stress regulates the expression of primary and specialized metabolism genes at the transcriptional level via transcription factors binding to specific cis-elements. The presence of varied cis-element signatures upstream to different stress-responsive genes and their transcription factor binding patterns provide a prospective molecular link among diverse metabolic pathways...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27929123/rnai-mediated-mortality-of-the-whitefly-through-transgenic-expression-of-double-stranded-rna-homologous-to-acetylcholinesterase-and-ecdysone-receptor-in-tobacco-plants
#3
Hassan Jamil Malik, Amir Raza, Imran Amin, Jodi A Scheffler, Brian E Scheffler, Judith K Brown, Shahid Mansoor
The whitefly Bemisia tabaci (Genn.) is a pest and vector of plant viruses to crop and ornamental plants worldwide. Using RNA interference (RNAi) to down regulate whitefly genes by expressing their homologous double stranded RNAs in plants has great potential for management of whiteflies to reduce plant virus disease spread. Using a Tobacco rattle virus-derived plasmid for in planta transient expression of double stranded RNA (dsRNA) homologous to the acetylcholinesterase (AChE) and ecdysone receptor (EcR) genes of B...
December 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27926860/l-met-activates-arabidopsis-glr-ca-2-channels-upstream-of-ros-production-and-regulates-stomatal-movement
#4
Dongdong Kong, Heng-Cheng Hu, Eiji Okuma, Yuree Lee, Hui Sun Lee, Shintaro Munemasa, Daeshik Cho, Chuanli Ju, Leah Pedoeim, Barbara Rodriguez, Juan Wang, Wonpil Im, Yoshiyuki Murata, Zhen-Ming Pei, June M Kwak
Plant glutamate receptor homologs (GLRs) have long been proposed to function as ligand-gated Ca(2+) channels, but no in planta evidence has been provided. Here, we present genetic evidence that Arabidopsis GLR3.1 and GLR3.5 form Ca(2+) channels activated by L-methionine (L-Met) at physiological concentrations and regulate stomatal apertures and plant growth. The glr3.1/3.5 mutations resulted in a lower cytosolic Ca(2+) level, defective Ca(2+)-induced stomatal closure, and Ca(2+)-deficient growth disorder, all of which involved L-Met...
December 6, 2016: Cell Reports
https://www.readbyqxmd.com/read/27924543/evaluating-fumonisin-gene-expression-in-fusarium-verticillioides
#5
Valeria Scala, Ivan Visentin, Francesca Cardinale
Transcript levels of key genes in a biosynthetic pathway are often taken as a proxy for metabolite production. This is the case of FUM1, encoding the first dedicated enzyme in the metabolic pathway leading to the production of the mycotoxins Fumonisins by fungal species belonging to the genus Fusarium. FUM1 expression can be quantified by different methods; here, we detail a protocol based on quantitative reverse transcriptase polymerase chain reaction (RT-qPCR), by which relative or absolute transcript abundance can be estimated in Fusaria grown in vitro or in planta...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27924129/interplay-of-genes-in-plant-pathogen-interactions-in-planta-expression-and-docking-studies-of-a-beta-1-3-glucanase-gene-from-piper-colubrinum-and-a-glucanase-inhibitor-gene-from-phytophthora-capsici
#6
K Johnson George, O Rosana Babu, I P Vijesh Kumar, J Santhosh Eapen, M Anandaraj
Oomycete pathogen, Phytophthora capsici is devastating for black pepper (Piper nigrum L.) and causes foot rot disease at all stages of plant growth. Phytophthora secretes a glucanase inhibitor protein (GIP), which is capable of inhibiting defence proteins like endoglucanases. In this particular study Quantitative PCR analysis, molecular docking studies and analysis of sequences of Glucanase inhibitor protein and beta-1,3 glucanse genes were done mainly depending on the data derived from Phytophthora capsici whole genome sequencing and Piper colubrinum RNA-sequencing (RNA-Seq)...
October 2016: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/27923776/establishment-of-expression-in-the-shortroot-scarecrow-transcriptional-cascade-through-opposing-activities-of-both-activators-and-repressors
#7
Erin E Sparks, Colleen Drapek, Allison Gaudinier, Song Li, Mitra Ansariola, Ning Shen, Jessica H Hennacy, Jingyuan Zhang, Gina Turco, Jalean J Petricka, Jessica Foret, Alexander J Hartemink, Raluca Gordân, Molly Megraw, Siobhan M Brady, Philip N Benfey
Tissue-specific gene expression is often thought to arise from spatially restricted transcriptional cascades. However, it is unclear how expression is established at the top of these cascades in the absence of pre-existing specificity. We generated a transcriptional network to explore how transcription factor expression is established in the Arabidopsis thaliana root ground tissue. Regulators of the SHORTROOT-SCARECROW transcriptional cascade were validated in planta. At the top of this cascade, we identified both activators and repressors of SHORTROOT...
December 5, 2016: Developmental Cell
https://www.readbyqxmd.com/read/27918247/type-iv-effector-proteins-involved-in-the-sinorhizobium-medicago-symbiosis
#8
Matthew S Nelson, Chan Lan Chun, Michael Jay Sadowsky
In this study, we investigated genetic elements of the type IV secretion system (T4SS) found in Sinorhizobium and the role they play in symbiosis. Sinorhizobium meliloti and S. medicae each contain a putative T4SS, similar to that used by Agrobacterium tumefaciens during pathogenesis. The Cre reporter assay for translocation (CRAfT) system was used to validate potential effector proteins. Both S. meliloti and S. medicae contained the effector protein TfeA, which was translocated into the host plant. Sequence analysis revealed the presence of a nod box, involved in transcriptional activation of symbiosis-related genes, upstream of the transcriptional regulator (virG) in the Sinorhizobium T4SS...
December 5, 2016: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/27916887/cucumber-metallothionein-like-2-csmtl2-exhibits-metal-binding-properties
#9
Yu Pan, Yanglu Pan, Junpeng Zhai, Yan Xiong, Jinhua Li, Xiaobing Du, Chenggang Su, Xingguo Zhang
We identified a novel member of the metallothionein (MT) family, Cucumis sativus metallothionein-like 2 (CsMTL2), by screening a young cucumber fruit complementary DNA (cDNA) library. The CsMTL2 encodes a putative 77-amino acid Class II MT protein that contains two cysteine (Cys)-rich domains separated by a Cys-free spacer region. We found that CsMTL2 expression was regulated by metal stress and was specifically induced by Cd(2+) treatment. We investigated the metal-binding characteristics of CsMTL2 and its possible role in the homeostasis and/or detoxification of metals by heterologous overexpression in Escherichia coli cells...
November 30, 2016: Genes
https://www.readbyqxmd.com/read/27911943/enhanced-in-planta-fitness-through-adaptive-mutations-in-efpr-a-dual-regulator-of-virulence-and-metabolic-functions-in-the-plant-pathogen-ralstonia-solanacearum
#10
Anthony Perrier, Rémi Peyraud, David Rengel, Xavier Barlet, Emmanuel Lucasson, Jérôme Gouzy, Nemo Peeters, Stéphane Genin, Alice Guidot
Experimental evolution of the plant pathogen Ralstonia solanacearum, where bacteria were maintained on plant lineages for more than 300 generations, revealed that several independent single mutations in the efpR gene from populations propagated on beans were associated with fitness gain on bean. In the present work, novel allelic efpR variants were isolated from populations propagated on other plant species, thus suggesting that mutations in efpR were not solely associated to a fitness gain on bean, but also on additional hosts...
December 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27911046/verticillium-dahliae-lysm-effectors-differentially-contribute-to-virulence-on-plant-hosts
#11
Anja Kombrink, Hanna Rovenich, Xiaoqian Shi-Kunne, Eduardo Rojas-Padilla, Grardy C M van den Berg, Emmanouil Domazakis, Ronnie de Jonge, Dirk-Jan Valkenburg, Andrea Sánchez-Vallet, Michael F Seidl, Bart P H J Thomma
Chitin-binding LysM effectors contribute to virulence of various plant pathogenic fungi that are causal agents of foliar diseases. Here, we report on LysM effectors of the soil-borne fungal vascular wilt pathogen Verticillium dahliae. Comparative genomics revealed three core LysM effectors that are conserved in a collection of V. dahliae strains. Remarkably, and in contrast to the previously studied LysM effectors of other plant pathogens, no expression of core LysM effectors was monitored in planta in a taxonomically diverse panel of host plants...
December 2, 2016: Molecular Plant Pathology
https://www.readbyqxmd.com/read/27901078/asian-citrus-psyllid-rnai-pathway-rnai-evidence
#12
Clauvis N T Taning, Eduardo C Andrade, Wayne B Hunter, Olivier Christiaens, Guy Smagghe
Diaphorina citri, known as the Asian citrus psyllid, is an important pest of citrus because it transmits a phloem-limited bacteria strongly implicated in huanglongbing (citrus greening disease). Emerging biotechnologies, such as RNA interference, could provide a new sustainable and environmentally friendly strategy for the management of this pest. In this study, genome and functional analysis were performed to verify whether the RNAi core genes are present in the Asian psyllid genome and if the RNAi machinery could be exploited to develop a management strategy for this pest...
November 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27899535/a-tomato-universal-stress-protein-involved-in-oxidative-stress-is-a-cipk6-phosphorylation-target
#13
Emilio Gutierrez-Beltran, José María Personat, Fernando N de la Torre, Olga Del Pozo
Calcineurin B-like interacting protein kinases (CIPKs) decode calcium signals upon interaction with the calcium sensors calcineurin B like proteins (CBLs) into phosphorylation events that result into adaptation to environmental stresses. Few phosphorylation targets of CIPKs are known and therefore the molecular mechanisms underlying their downstream output responses are not fully understood. Tomato (Solanum lycopersicum) Cipk6 regulates immune and susceptible Programmed cell death (PCD) in immunity transforming Ca2+ signals into Reactive Oxygen Species (ROS) signaling...
November 29, 2016: Plant Physiology
https://www.readbyqxmd.com/read/27895938/understanding-colonization-and-proliferation-potential-of-endophytes-and-pathogen-in-planta-via-plating-polymerase-chain-reaction-and-ergosterol-assay
#14
Yiing Yng Chow, Sadequr Rahman, Adeline Su Yien Ting
This study aimed to establish the colonization behavior and proliferation potential of three endophytes and one pathogen Ganoderma boninense (Gb) introduced into oil palm ramets (host model). The endophytes selected were Diaporthe phaseolorum (WAA02), Trichoderma asperellum (T2), and Penicillium citrinum (BTF08). Ramets were first inoculated with 100 mL of fungal cells (10(6) cfu mL(-)(1)) via soil drenching. For the next 7 days, ramets were sampled and subjected to three different assays to detect and identify fungal colonization, and establish their proliferation potential in planta...
January 2017: Journal of Advanced Research
https://www.readbyqxmd.com/read/27895701/a-dna-based-real-time-pcr-assay-for-robust-growth-quantification-of-the-bacterial-pathogen-pseudomonas-syringae-on-arabidopsis-thaliana
#15
Annegret Ross, Imre E Somssich
BACKGROUND: The interaction of Pseudomonas syringae with Arabidopsis is one of the most commonly used systems to study various bacterial-host interrelationships. Currently, most studies are based on the growth quantification of the pathogen to characterize resistance or virulence targets. However, the standard available method for determining bacterial proliferation in planta is laborious and has several limitations. RESULTS: Here we present an alternative robust approach, which is based on the quantification of bacterial DNA by real-time PCR...
2016: Plant Methods
https://www.readbyqxmd.com/read/27884263/non-weight-bearing-and-weight-bearing-ultrasonography-of-select-foot-muscles-in-young-asymptomatic-participants-a-descriptive-and-reliability-study
#16
Patrick J Battaglia, Ross Mattox, Brett Winchester, Norman W Kettner
OBJECTIVE: The primary aim of this study was to determine the reliability of diagnostic ultrasound imaging for select intrinsic foot muscles using both non-weight-bearing and weight-bearing postures. Our secondary aim was to describe the change in muscle cross-sectional area (CSA) and dorsoplantar thickness when bearing weight. METHODS: An ultrasound examination was performed with a linear ultrasound transducer operating between 9 and 12 MHz. Long-axis and short-axis ultrasound images of the abductor hallucis, flexor digitorum brevis, and quadratus plantae were obtained in both the non-weight-bearing and weight-bearing postures...
November 2016: Journal of Manipulative and Physiological Therapeutics
https://www.readbyqxmd.com/read/27875982/comparative-genomics-to-explore-phylogenetic-relationship-cryptic-sexual-potential-and-host-specificity-of-rhynchosporium-species-on-grasses
#17
Daniel Penselin, Martin Münsterkötter, Susanne Kirsten, Marius Felder, Stefan Taudien, Matthias Platzer, Kevin Ashelford, Konrad H Paskiewicz, Richard J Harrison, David J Hughes, Thomas Wolf, Ekaterina Shelest, Jenny Graap, Jan Hoffmann, Claudia Wenzel, Nadine Wöltje, Kevin M King, Bruce D L Fitt, Ulrich Güldener, Anna Avrova, Wolfgang Knogge
BACKGROUND: The Rhynchosporium species complex consists of hemibiotrophic fungal pathogens specialized to different sweet grass species including the cereal crops barley and rye. A sexual stage has not been described, but several lines of evidence suggest the occurrence of sexual reproduction. Therefore, a comparative genomics approach was carried out to disclose the evolutionary relationship of the species and to identify genes demonstrating the potential for a sexual cycle. Furthermore, due to the evolutionary very young age of the five species currently known, this genus appears to be well-suited to address the question at the molecular level of how pathogenic fungi adapt to their hosts...
November 22, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27871149/pstha5a23-a-candidate-effector-from-the-obligate-biotrophic-pathogen-puccinia-striiformis-f-sp-tritici-is-involved-in-plant-defense-suppression-and-rust-pathogenicity
#18
Yulin Cheng, Kuan Wu, Juanni Yao, Shumin Li, Xiaojie Wang, Lili Huang, Zhensheng Kang
During the infection of host plants, pathogens can deliver virulence-associated "effector" proteins to promote plant susceptibility. However, little is known about effector function in the obligate biotrophic pathogen Puccinia striiformis f. sp. tritici (Pst) that is an important fungal pathogen in wheat production worldwide. Here, we report our findings on an in planta highly induced candidate effector from Pst, PSTha5a23. The PSTha5a23 gene is unique to Pst and shows a low level of intra-species polymorphism...
November 21, 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/27870066/a-nonnative-and-a-native-fungal-plant-pathogen-similarly-stimulate-ectomycorrhizal-development-but-are-perceived-differently-by-a-fungal-symbiont
#19
Elisa Zampieri, Luana Giordano, Guglielmo Lione, Alfredo Vizzini, Fabiano Sillo, Raffaella Balestrini, Paolo Gonthier
The effects of plant symbionts on host defence responses against pathogens have been extensively documented, but little is known about the impact of pathogens on the symbiosis and if such an impact may differ for nonnative and native pathogens. Here, this issue was addressed in a study of the model system comprising Pinus pinea, its ectomycorrhizal symbiont Tuber borchii, and the nonnative and native pathogens Heterobasidion irregulare and Heterobasidion annosum, respectively. In a 6-month inoculation experiment and using both in planta and gene expression analyses, we tested the hypothesis that H...
November 21, 2016: New Phytologist
https://www.readbyqxmd.com/read/27856908/characterization-of-a-dynamic-metabolon-producing-the-defense-compound-dhurrin-in-sorghum
#20
Tomas Laursen, Jonas Borch, Camilla Knudsen, Krutika Bavishi, Federico Torta, Helle Juel Martens, Daniele Silvestro, Nikos S Hatzakis, Markus R Wenk, Timothy R Dafforn, Carl Erik Olsen, Mohammed Saddik Motawia, Björn Hamberger, Birger Lindberg Møller, Jean-Etienne Bassard
Metabolic highways may be orchestrated by the assembly of sequential enzymes into protein complexes, or metabolons, to facilitate efficient channeling of intermediates and to prevent undesired metabolic cross-talk while maintaining metabolic flexibility. Here we report the isolation of the dynamic metabolon that catalyzes the formation of the cyanogenic glucoside dhurrin, a defense compound produced in sorghum plants. The metabolon was reconstituted in liposomes, which demonstrated the importance of membrane surface charge and the presence of the glucosyltransferase for metabolic channeling...
November 18, 2016: Science
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