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https://www.readbyqxmd.com/read/28074922/soybean-cyclophilin-gmcyp1-interacts-with-an-isoflavonoid-regulator-gmmyb176
#1
Hemanta Raj Mainali, Arun Kumaran Anguraj Vadivel, Xuyan Li, Mark Gijzen, Sangeeta Dhaubhadel
Cyclophilins (CYPs) belong to the immunophilin superfamily with peptidyl-prolyl cis-trans isomerase (PPIase) activity. They catalyze the interconversion of the cis- and trans-rotamers of the peptidyl-prolyl amide bond of peptides. A yeast-two-hybrid screening using the isoflavonoid regulator GmMYB176 as bait identified GmCYP1 as one of the interacting proteins in soybean embryos. GmCYP1 localizes both in the nucleus and cytoplasm, and interacts in planta with GmMYB176, in the nucleus, and with SGF14l (a soybean 14-3-3 protein) in the nucleus and the cytoplasm...
January 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28073984/transcription-factors-pverf15-and-pvmtf-1-form-a-cadmium-stress-transcriptional-pathway
#2
Tingting Lin, Wanning Yang, Wen Lu, Ying Wang, Xiaoting Qi
In plants, cadmium (Cd)-responsive transcription factors are key downstream effectors of Cd stress transcriptional pathways, which are capable of converging Cd stress signals through triggering the expression of Cd-detoxification genes. However, the upstream transcriptional regulatory pathways that modulate their responses to Cd are less clear. Previously, we identified a bean (Phaseolus vulgaris) metal response element-binding transcription factor1 (PvMTF-1) that responds to Cd and confers Cd tolerance in planta...
January 10, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28072559/control-of-endophytic-frankia-sporulation-by-alnus-nodule-metabolites
#3
Anne-Emmanuelle Hay De-Bettignies, Hasna Boubakri, Antoine Buonomo, Marjolaine Rey, Guillaume Meiffren, Laetitia Cotin-Galvan, Gilles Comte, Aude Herrera-Belaroussi
A unique case of microbial symbiont capable of dormancy within its living host cells has been reported in actinorhizal symbioses: some Frankia strains, named Sp+, are able to sporulate inside plant cells, contrarily to Sp- strains. The presence of metabolically slowed down bacterial structures in host cells alters our understanding of symbiosis based on reciprocal benefits between both partners, and its impact on the symbiotic processes remains unknown. The present work reports a metabolomic study of Sp+ and Sp- nodules (from Alnus glutinosa), in order to highlight variabilities associated with in-planta sporulation...
January 10, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28071976/precise-flexible-and-affordable-gene-stacking-for-crop-improvement
#4
Weiqiang Chen, David W Ow
Addendum to: Hou L, Yau YY, Wei J, Han Z, Dong Z, Ow DW. An open-source system for in planta gene stacking by Bxb1 and Cre recombinases. Mol Plant, 2014; 7(12):1756-1765. doi:10.1093/mp/ssu107; Li R, Han Z, Hou L, Kaur G, Yin Q, Ow DW. Method for Biolistic Site-Specific Integration in Plants Catalyzed by Bxb1 Integrase. In: Murata M, editor. Chromosome and Genomic Engineering in Plants: Methods and Protocols, Methods in Molecular Biology. 2016; p.15-30; and Chen W, Ow DW. Protocol for In Vitro Stacked Molecules Compatible with In Vivo Recombinase-Mediated Gene Stacking...
January 10, 2017: Bioengineered
https://www.readbyqxmd.com/read/28063194/evolution-of-copper-resistance-in-the-kiwifruit-pathogen-pseudomonas-syringae-pv-actinidiae-through-acquisition-of-integrative-conjugative-elements-and-plasmids
#5
Elena Colombi, Christina Straub, Sven Künzel, Matthew D Templeton, Honour C McCann, Paul B Rainey
Horizontal gene transfer can precipitate rapid evolutionary change. In 2010 the global pandemic of kiwifruit canker disease caused by Pseudomonas syringae pv. actinidiae (Psa) reached New Zealand. At the time of introduction, the single clone responsible for the outbreak was sensitive to copper, however, analysis of a sample of isolates taken in 2015 and 2016 showed that a quarter were copper resistant. Genome sequences of seven strains showed that copper resistance - comprising czc/cusABC and copABCD systems - along with resistance to arsenic and cadmium, was acquired via uptake of integrative conjugative elements (ICEs), but also plasmids...
January 7, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28060406/a-simple-protoplast-based-method-for-screening-potent-artificial-mirna-for-maximal-gene-silencing-in-arabidopsis
#6
Nannan Zhang, Dandan Zhang, Jian-Feng Li
The unpredictability of in planta silencing efficiency of artificial micro RNAs (amiRNAs) has greatly limited the application of the amiRNA technology in basic and applied plant research. This unit describes a simple and robust method called the epitope-tagged protein-based amiRNA (ETPamir) screen for identifying the most effective amiRNA for silencing a particular Arabidopsis gene. After selecting three to four amiRNA candidates designed by the WMD3 web server for the target gene, the silencing efficiencies of the amiRNA candidates can be compared by co-expressing individual amiRNAs with the target mRNA encoding an epitope-tagged target protein in Arabidopsis mesophyll protoplasts...
January 5, 2017: Current Protocols in Molecular Biology
https://www.readbyqxmd.com/read/28056069/engineering-potato-starch-with-a-higher-phosphate-content
#7
Xuan Xu, Xing-Feng Huang, Richard G F Visser, Luisa M Trindade
Phosphate esters are responsible for valuable and unique functionalities of starch for industrial applications. Also in the cell phosphate esters play a role in starch metabolism, which so far has not been well characterized in storage starch. Laforin, a human enzyme composed of a carbohydrate-binding module and a dual-specificity phosphatase domain, is involved in the dephosphorylation of glycogen. To modify phosphate content and better understand starch (de)phosphorylation in storage starch, laforin was engineered and introduced into potato (cultivar Kardal)...
2017: PloS One
https://www.readbyqxmd.com/read/28056034/btb-back-domain-protein-pob1-suppresses-immune-cell-death-by-targeting-ubiquitin-e3-ligase-pub17-for-degradation
#8
Beatriz Orosa, Qin He, Joelle Mesmar, Eleanor M Gilroy, Hazel McLellan, Chengwei Yang, Adam Craig, Mark Bailey, Cunjin Zhang, Jonathan David Moore, Petra C Boevink, Zhendong Tian, Paul R J Birch, Ari Sadanandom
Hypersensitive response programmed cell death (HR-PCD) is a critical feature in plant immunity required for pathogen restriction and prevention of disease development. The precise control of this process is paramount to cell survival and an effective immune response. The discovery of new components that function to suppress HR-PCD will be instrumental in understanding the regulation of this fundamental mechanism. Here we report the identification and characterisation of a BTB domain E3 ligase protein, POB1, that functions to suppress HR-PCD triggered by evolutionarily diverse pathogens...
January 5, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28050830/live-imaging-of-developing-xylem-in-planta
#9
Raymond Wightman
The xylem vessel develops from a long cylindrical cell that deposits a patterned secondary wall and ending with programmed cell death. The dynamic arrangement of cell wall enzymes, membranes and the cytoskeleton can be recorded using live fluorescence imaging. The protocol presented here focuses upon the microscopy of intracellular components in developing vessels of the root using either epifluorescent or confocal microscopes.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28050737/identification-and-characterization-of-bamboo-mosaic-virus-isolates-from-a-naturally-occurring-coinfection-in-bambusa-xiashanensis
#10
Wenwu Lin, Lu Wang, Wenkai Yan, Lingli Chen, Huihuang Chen, Wenting Yang, Maohui Guo, Zujian Wu, Liang Yang, Lianhui Xie
Bamboo mosaic virus (BaMV) is a well-characterized virus and a model of virus-host interaction in plants. Here, we identified naturally occurring BaMV isolates from Fujian Province, China and furthermore describe a naturally occurring BaMV coinfection in bamboo (Bambusa xiashanensis) plants. Two different types of BaMV were identified, represented by isolates BaMV-XSNZHA7 (X7) and BaMV-XSNZHA10 (X10). The phylogenetic relationships between X7- and X10-like isolates and published BaMV isolates were determined based on genomic RNA and amino acid sequences...
January 3, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28040774/comparative-omics-of-the-fusarium-fujikuroi-species-complex-highlights-differences-in-genetic-potential-and-metabolite-synthesis
#11
Eva-Maria Niehaus, Martin Münsterkötter, Robert H Proctor, Daren W Brown, Amir Sharon, Yifat Idan, Liat Oren-Young, Christian M Sieber, Ondřej Novák, Aleš Pěnčík, Danuše Tarkowská, Kristýna Hromadová, Stanley Freeman, Marcel Maymon, Meirav Elazar, Sahar A Youssef, El Said M El-Shabrawy, Abdel Baset A Shalaby, Petra Houterman, Nelson L Brock, Immo Burkhardt, Elena A Tsavkelova, Jeroen S Dickschat, Petr Galuszka, Ulrich Güldener, Bettina Tudzynski
Species of the Fusarium fujikuroi species complex (FFC) cause a wide spectrum of often devastating diseases on diverse agricultural crops, including coffee, fig, mango, maize, rice, and sugarcane. Although species within the FFC are difficult to distinguish by morphology, and their genes often share 90% sequence similarity, they can differ in host plant specificity and life style. FFC species can also produce structurally diverse secondary metabolites (SMs), including the mycotoxins fumonisins, fusarins, fusaric acid, and beauvericin, and the phytohormones gibberellins, auxins, and cytokinins...
December 31, 2016: Genome Biology and Evolution
https://www.readbyqxmd.com/read/28039930/interacting-protein-partners-of-arabidopsis-rna-binding-protein-atrbp45b
#12
Meenakumari Muthuramalingam, Yixing Wang, Yongfang Li, Ramamurthy Mahalingam
• RNA binding proteins (RBPs), important players in post-transcriptional gene regulation usually exist in ribonuclear complexes. However, even in model systems like Arabidopsis characterization of RBP associated proteins are limited. In this study, we investigated the interacting proteins of the Arabidopsis AtRBP45b, which is involved in stress signaling. • In vivo localization of AtRBP45b was conducted using 35S-GFP. FLAG-tagged AtRBP45b under the control of 35S promoter in the Atrbp45b-1 mutant background was used to pull down AtRBP45b interacting proteins...
December 31, 2016: Plant Biology
https://www.readbyqxmd.com/read/28034799/impact-of-biotic-and-abiotic-factors-on-the-expression-of-fungal-effector-encoding-genes-in-axenic-growth-conditions
#13
Michel Meyer, Salim Bourras, Julie Gervais, Karine Labadie, Corinne Cruaud, Marie-Hélène Balesdent, Thierry Rouxel
In phytopathogenic fungi, the expression of hundreds of small secreted protein (SSP)-encoding genes is induced upon primary infection of plants while no or a low level of expression is observed during vegetative growth. In some species such as Leptosphaeria maculans, this coordinated in-planta upregulation of SSP-encoding genes expression relies on an epigenetic control but the signals triggering gene expression in-planta are unknown. In the present study, biotic and abiotic factors that may relieve suppression of SSP-encoding gene expression during axenic growth of L...
December 26, 2016: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/28034789/bibac-gw-based-vectors-for-generating-reporter-lines-for-site-specific-genome-editing-in-planta
#14
Damar Tri Anggoro, Mariliis Tark-Dame, Aimee Walmsley, Rurika Oka, Mara de Sain, Maike Stam
When generating transgenic plants, one of the objectives is to achieve stable expression of the transgene. Transgene silencing can be avoided by single copy integration of the transgene. Binary systems that predominantly result in single copy integrations, such as BIBAC vectors, are also single-copy in E. coli, the organism in which the T-DNA to be delivered to the plant is assembled. Although a low-copy number is important for stable maintenance of large DNA fragments in E. coli, it hampers cloning into the vector due to a low DNA yield...
December 26, 2016: Plasmid
https://www.readbyqxmd.com/read/28032363/investigation-of-the-crosstalk-between-the-flg22-and-the-uv-b-induced-flavonol-pathway-in-arabidopsis-thaliana-seedlings
#15
Zheng Zhou, Dirk Schenke, Ying Miao, Daguang Cai
In Arabidopsis cell culture, we have shown that flavonol metabolite accumulation depends on expression of the flavonol pathway genes (FPGs), which are up-regulated by UV-B irradiation but repressed during microbe-associated molecular pattern (MAMP) -triggered immunity (MTI) induced by the bacterial elicitor flg22. The suppression of flavonoid production during MTI is believed to allow the plant focusing its metabolism on the pathogen defense by directing phenylalanine resources from UV-B protective flavonol production towards production of phytoalexins and cell wall fortification by lignin incorporation...
December 29, 2016: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28024547/metrics-of-rhamnogalacturonan-i-with-%C3%AE-1%C3%A2-4-linked-galactan-side-chains-and-structural-basis-for-its-self-aggregation
#16
Olga N Makshakova, Tatyana A Gorshkova, Polina V Mikshina, Yuriy F Zuev, Serge Perez
Within the family of plant cell wall polysaccharides rhamnogalacturonans I are the most diverse and structurally complex members. In present study we characterize the 3-dimensional structures and dynamic features of the constituents of RG-I along MD trajectories. It is demonstrated that extended threefold helical structure of the rhamnogalacturonan linear backbone is the most energetically favorable motif. Branching helps to stabilize a conformer of the backbone twisted along 1→2 glycosidic linkage triggering the orientation of long side chains without altering the extended overall backbone chain conformation...
February 20, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28019063/interaction-of-transgenic-and-natural-insect-resistance-mechanisms-against-spodoptera-littoralis-in-cotton
#17
Steffen Hagenbucher, Michael Eisenring, Michael Meissle, Jörg Romeis
BACKGROUND: Insect-resistant transgenic plants that express insecticidal Cry proteins from Bacillus thuringiensis (Bt), are grown on millions of hectares worldwide. While these proteins are efficient in controlling key lepidopteran pests, not all pests are affected and the development of resistance in target pests is always a danger. These short-comings could be supplemented by exploiting the natural insect resistance of cotton, especially inducible terpenoids like gossypol. RESULTS: To assess the potential of gossypol in supplementing Cry proteins as a resistance-trait, we conducted a range of feeding assays with Spodoptera littoralis using artificial diet with defined amounts of Cry proteins and gossypol...
December 26, 2016: Pest Management Science
https://www.readbyqxmd.com/read/28018392/narrow-leafed-lupin-lupinus-angustifolius-%C3%AE-1-and-%C3%AE-6-conglutin-proteins-exhibit-antifungal-activity-protecting-plants-against-necrotrophic-pathogen-induced-damage-from-sclerotinia-sclerotiorum-and-phytophthora-nicotianae
#18
Jose C Jimenez-Lopez, Su Melser, Kathleen DeBoer, Louise F Thatcher, Lars G Kamphuis, Rhonda C Foley, Karam B Singh
Vicilins (7S globulins) are seed storage proteins and constitute the main protein family in legume seeds, particularly in narrow-leafed lupin (Lupinus angustifolius L.; NLL), where seven vicilin genes, called β1- to β7-conglutin have been identified. Vicilins are involved in germination processes supplying amino acids for seedling growth and plant development, as well as in some cases roles in plant defense and protection against pathogens. The roles of NLL β-conglutins in plant defense are unknown. Here the potential role of five NLL β-conglutin family members in protection against necrotrophic fungal pathogens was investigated and it was demonstrated that recombinant purified 6xHis-tagged β1- and β6-conglutin proteins exhibited the strongest in vitro growth inhibitory activity against a range of necrotrophic fungal pathogens compared to β2, β3, and β4 conglutins...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28018312/enzyme-mediated-quenching-of-the-pseudomonas-quinolone-signal-pqs-promotes-biofilm-formation-of-pseudomonas-aeruginosa-by-increasing-iron-availability
#19
Beatrix Tettmann, Christine Niewerth, Frank Kirschhöfer, Anke Neidig, Andreas Dötsch, Gerald Brenner-Weiss, Susanne Fetzner, Joerg Overhage
The 2-alkyl-3-hydroxy-4(1H)-quinolone 2,4-dioxygenase HodC was previously described to cleave the Pseudomonas quinolone signal, PQS, which is exclusively used in the complex quorum sensing (QS) system of Pseudomonas aeruginosa, an opportunistic pathogen employing QS to regulate virulence and biofilm development. Degradation of PQS by exogenous addition of HodC to planktonic cells of P. aeruginosa attenuated production of virulence factors, and reduced virulence in planta. However, proteolytic cleavage reduced the efficacy of HodC...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28017611/regulation-of-differentiation-of-nitrogen-fixing-bacteria-by-microsymbiont-targeting-of-plant-thioredoxin-s1
#20
Carolina Werner Ribeiro, Fabien Baldacci-Cresp, Olivier Pierre, Marie Larousse, Sofiane Benyamina, Annie Lambert, Julie Hopkins, Claude Castella, Julie Cazareth, Geneviève Alloing, Eric Boncompagni, Jérémy Couturier, Peter Mergaert, Pascal Gamas, Nicolas Rouhier, Françoise Montrichard, Pierre Frendo
Legumes associate with rhizobia to form nitrogen (N2)-fixing nodules, which is important for plant fitness [1, 2]. Medicago truncatula controls the terminal differentiation of Sinorhizobium meliloti into N2-fixing bacteroids by producing defensin-like nodule-specific cysteine-rich peptides (NCRs) [3, 4]. The redox state of NCRs influences some biological activities in free-living bacteria, but the relevance of redox regulation of NCRs in planta is unknown [5, 6], although redox regulation plays a crucial role in symbiotic nitrogen fixation [7, 8]...
December 19, 2016: Current Biology: CB
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