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Zebrafish brain development

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https://www.readbyqxmd.com/read/28100783/dual-functional-roles-of-molecular-beacon-a-microrna-detector-and-inhibitor
#1
Wai Ming Li, Ching-Man Chan, Andrew L Miller, Chow H Lee
MicroRNAs are essential in many cellular processes. The ability to detect microRNAs is important for understanding its function and biogenesis. This study is aimed at using a molecular beacon to detect miR-430 in developing zebrafish embryos as a proof of principle. miR-430 is crucial for the clearance of maternal mRNA during maternal zygotic transition (MZT) in embryonic development. Despite its known function, the temporal and spatial expression of miR-430 remains unclear. We used various imaging techniques, including laser scanning confocal microscopy, spinning disk and lightsheet microscopy, to study the localization of miR-430 and any developmental defects possibly caused by the molecular beacon...
January 18, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28098234/maternal-cortisol-stimulates-neurogenesis-and-affects-larval-behaviour-in-zebrafish
#2
Carol Best, Deborah M Kurrasch, Mathilakath M Vijayan
Excess glucocorticoid transferred from stressed mother to the embryo affects developing vertebrate offspring, but the underlying programming events are unclear. In this study, we tested the hypothesis that increased zygotic glucocorticoid deposition, mimicking a maternal stress scenario, modifies early brain development and larval behaviour in zebrafish (Danio rerio). Cortisol was microinjected into the yolk at one cell-stage, to mimic maternal transfer, and the larvae [96 hours post-fertilization (hpf)] displayed increased activity in light and a reduction in thigmotaxis, a behavioural model for anxiety, suggesting an increased propensity for boldness...
January 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28092494/zebrafish-are-able-to-detect-ethanol-in-their-environment
#3
Steven Tran, Hayden Chow, Benjamin Tsang, Amanda Facciol, Prabhlene Gandhi, Priyanka Desai, Robert Gerlai
Zebrafish have become a popular animal model for studying the development of alcohol addiction. Several behavioral paradigms for studying alcohol addiction have been developed for zebrafish, including conditioned place preference, alcohol-induced tolerance, and withdrawal. However, alcohol choice preference tasks have not been established in zebrafish as of yet. The ability of zebrafish to detect alcohol in their environment is required in alcohol choice or preference tasks. To our knowledge, it is currently unknown whether zebrafish are able to detect alcohol in their environment immediately following bath immersion...
January 16, 2017: Zebrafish
https://www.readbyqxmd.com/read/28065605/early-commissural-diencephalic-neurons-control-habenular-axon-extension-and-targeting
#4
Carlo A Beretta, Nicolas Dross, Luca Guglielmi, Peter Bankhead, Marina Soulika, Jose A Gutierrez-Triana, Alessio Paolini, Lucia Poggi, Julien Falk, Soojin Ryu, Marika Kapsimali, Ulrike Engel, Matthias Carl
Most neuronal populations form on both the left and right sides of the brain. Their efferent axons appear to grow synchronously along similar pathways on each side, although the neurons or their environment often differ between the two hemispheres [1-4]. How this coordination is controlled has received little attention. Frequently, neurons establish interhemispheric connections, which can function to integrate information between brain hemispheres (e.g., [5]). Such commissures form very early, suggesting their potential developmental role in coordinating ipsilateral axon navigation during embryonic development [4]...
December 29, 2016: Current Biology: CB
https://www.readbyqxmd.com/read/28051181/the-larvicide-pyriproxyfen-blamed-during-the-zika-virus-outbreak-does-not-cause-microcephaly-in-zebrafish-embryos
#5
Stefania Dzieciolowska, Anne-Laure Larroque, Elizabeth-Ann Kranjec, Pierre Drapeau, Eric Samarut
Although the zika virus (ZIKV) has now been strongly correlated with emerging cases of microcephaly in the Americas, suspicions have been raised regarding the use of pyriproxyfen, a larvicide that prevents mosquito development, in drinking water. The effects of this compound on neurodevelopment have not yet been addressed specifically in vertebrates. As a result, we aimed at addressing the effects, if any, of pyriproxyfen on neurodevelopment in the zebrafish embryo as a vertebrate model. Using zebrafish transgenic lines expressing GFP in different cell populations (elavl3 in newborn neurons, gfap and nestin in neural stem cells), we focused on the analysis of whole embryonic brain volume after confocal 3D-reconstruction and the quantification of purified neural stem cells during early neurodevelopment by FACS-cell sorting from whole in vivo embryos...
January 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28036051/developmental-neurotoxicity-of-methamidophos-in-the-embryo-larval-stages-of-zebrafish
#6
Xiaowei He, Jiawei Gao, Tianyu Dong, Minjian Chen, Kun Zhou, Chunxin Chang, Jia Luo, Chao Wang, Shoulin Wang, Daozhen Chen, Zuomin Zhou, Ying Tian, Yankai Xia, Xinru Wang
Methamidophos is a representative organophosphate insecticide. The knowledge of its developmental neurotoxicity is limited, especially for zebrafish in the early stages of their life. Four hour post-fertilization (hpf) zebrafish embryos were exposed to several environmentally relevant concentrations of methamidophos (0, 25, and 500 μg/L) for up to 72 hpf. Locomotor behavior was then studied in the zebrafish larvae at this timepoint. Acridine orange (AO) staining was carried out in the zebrafish larvae, and the mRNA levels of genes associated with neural development (mbp and syn2a) were analyzed by reverse transcription-polymerase chain reaction (RT-PCR)...
December 28, 2016: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/27979830/molecular-insights-into-adgra2-gpr124-and-reck-intracellular-trafficking
#7
Naguissa Bostaille, Anne Gauquier, Laure Twyffels, Benoit Vanhollebeke
Adgra2, formerly known as Gpr124, is a key regulator of cerebrovascular development in vertebrates. Together with the GPI-anchored glycoprotein Reck, this adhesion GPCR (aGPCR) stimulates Wnt7-dependent Wnt/β-catenin signaling to promote brain vascular invasion in an endothelial cell-autonomous manner. Adgra2 and Reck have been proposed to assemble a receptor complex at the plasma membrane, but the molecular modalities of their functional synergy remain to be investigated. In particular, as typically found in aGPCRs, the ectodomain of Adgra2 is rich in protein-protein interaction motifs whose contributions to receptor function are unknown...
December 15, 2016: Biology Open
https://www.readbyqxmd.com/read/27978793/generation-of-alzheimer-s-disease-transgenic-zebrafish-expressing-human-app-mutation-under-control-of-zebrafish-appb-promotor
#8
Yun-Zhu Pu, Liang Liang, Ai-Ling Fu, Yan Liu, Lan Sun, Qian Li, Dan Wu, Man-Ji Sun, Ying-Ge Zhang, Bao-Quan Zhao
Amyloid peptide precursor (APP) as the precursor protein of peptide beta-amyloid (β-amyloid, Aβ), which is thought to play a central role in the pathogenesis of Alzheimer's disease (AD), also has an important effect on the development and progression of AD. Through knocking-in APP gene in animals, numerous transgenic AD models have been set up for the investigation of the mechanisms behind AD pathogenesis and the screening of anti-AD drugs. However, there are some limitations to these models and here is a need for such an AD model that is economic as well as has satisfactory genetic homology with human...
December 1, 2016: Current Alzheimer Research
https://www.readbyqxmd.com/read/27936201/sterol-o-acyltransferase-2-contributes-to-the-yolk-cholesterol-trafficking-during-zebrafish-embryogenesis
#9
Nai-Yun Chang, Yen-Ju Chan, Shih-Torng Ding, Yen-Hua Lee, Wei-Chun HuangFu, I-Hsuan Liu
To elucidate whether Sterol O-acyltransferase (Soat) mediates the absorption and transportation of yolk lipids to the developing embryo, zebrafish soat1 and soat2 were cloned and studied. In the adult zebrafish, soat1 was detected ubiquitously while soat2 mRNA was detected specifically in the liver, intestine, brain and testis. Whole mount in situ hybridization demonstrated that both soat1 and soat2 expressed in the yolk syncytial layer, hatching gland and developing cardiovascular as well as digestive systems, suggesting that Soats may play important roles in the lipid trafficking and utilization during embryonic development...
2016: PloS One
https://www.readbyqxmd.com/read/27935819/a-novel-brain-tumour-model-in-zebrafish-reveals-the-role-of-yap-activation-in-mapk-pi3k-induced-malignant-growth
#10
Marie Mayrhofer, Victor Gourain, Markus Reischl, Pierre Affaticati, Arnim Jenett, Jean-Stephane Joly, Matteo Benelli, Francesca Demichelis, Pietro Luigi Poliani, Dirk Sieger, Marina Mione
Somatic mutations activating MAPK/PI3K signalling play a pivotal role in both tumours and brain developmental disorders. We developed a zebrafish model of brain tumour based on somatic expression of oncogenes that activate MAPK/PI3K signalling in neural progenitor cells. HRAS(V12) was the most effective in inducing both heterotopia and invasive tumours. Tumours, but not heterotopias, require persistent activation of phospho‑(p)ERK and express a gene signature similar to the mesenchymal glioblastoma subtype, with a strong YAP component...
November 24, 2016: Disease Models & Mechanisms
https://www.readbyqxmd.com/read/27933364/behavioral-alterations-of-zebrafish-larvae-after-early-embryonic-exposure-to-ketamine
#11
Luís M Félix, Luís M Antunes, Ana M Coimbra, Ana M Valentim
RATIONALE: Ketamine has been associated with pediatric risks that include neurocognitive impairment and long-term behavioral disorders. However, the neurobehavioral effects of ketamine exposure in early development remain uncertain. OBJECTIVES: This study aimed to test stage- and dose-dependent effects of ketamine exposure on certain brain functions by evaluating alterations in locomotion, anxiety-like and avoidance behaviors, as well as socialization. METHODS: Embryos were exposed to different concentrations of ketamine (0, 0...
December 8, 2016: Psychopharmacology
https://www.readbyqxmd.com/read/27919782/understanding-zebrafish-cognition
#12
REVIEW
Darya A Meshalkina, Marina N Kizlyk, Elana V Kysil, Adam D Collier, David J Echevarria, Murilo S Abreu, Leonardo J G Barcellos, Cai Song, Allan V Kalueff
Zebrafish (Danio rerio) are rapidly becoming a popular model organism in translational and cognitive neuroscience research. Both larval and adult zebrafish continue to increase our understanding of cognitive mechanisms and their genetic and pharmacological modulation. Here, we discuss the developing utility of zebrafish in understanding cognitive phenotypes and their deficits, relevant to a wide range human brain disorders. We also discuss the potential of zebrafish models for high-throughput genetic mutant and small molecule screening (e...
December 2, 2016: Behavioural Processes
https://www.readbyqxmd.com/read/27899647/spliceosomal-protein-eftud2-mutation-leads-to-p53-dependent-apoptosis-in-zebrafish-neural-progenitors
#13
Lei Lei, Shou-Yu Yan, Ran Yang, Jia-Yu Chen, Yumei Li, Ye Bu, Nannan Chang, Qinchao Zhou, Xiaojun Zhu, Chuan-Yun Li, Jing-Wei Xiong
Haploinsufficiency of EFTUD2 (Elongation Factor Tu GTP Binding Domain Containing 2) is linked to human mandibulofacial dysostosis, Guion-Almeida type (MFDGA), but the underlying cellular and molecular mechanisms remain to be addressed. We report here the isolation, cloning and functional analysis of the mutated eftud2 (snu114) in a novel neuronal mutant fn10a in zebrafish. This mutant displayed abnormal brain development with evident neuronal apoptosis while the development of other organs appeared less affected...
November 28, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27892483/down-regulation-of-coasy-the-gene-associated-with-nbia-vi-reduces-bmp-signaling-perturbs-dorso-ventral-patterning-and-alters-neuronal-development-in-zebrafish
#14
Deepak Khatri, Daniela Zizioli, Natascia Tiso, Nicola Facchinello, Sara Vezzoli, Alessandra Gianoncelli, Maurizio Memo, Eugenio Monti, Giuseppe Borsani, Dario Finazzi
Mutations in Pantothenate kinase 2 and Coenzyme A (CoA) synthase (COASY), genes involved in CoA biosynthesis, are associated with rare neurodegenerative disorders with brain iron accumulation. We showed that zebrafish pank2 gene plays an essential role in brain and vasculature development. Now we extended our study to coasy. The gene has high level of sequence identity with the human ortholog and is ubiquitously expressed from the earliest stages of development. The abrogation of its expression led to strong reduction of CoA content, high lethality and a phenotype resembling to that of dorsalized mutants...
November 28, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27878267/distribution-pharmacokinetics-and-primary-metabolism-model-of-tramadol-in-zebrafish
#15
Huiqin Zhuo, Hongwei Jin, Huifang Peng, Heqing Huang
The current study aimed to develop a rapid, robust and adequately sensitive method for simultaneous determination of the concentration of tramadol and its active metabolites in zebrafish. The pharmacokinetic and elimination pattern of tramadol and its major phase I metabolites following oral or intramuscular administration in zebrafish tissues was achieved using electrospray ionization‑quadrupole‑time of flight/mass spectrometry (ESI‑Q‑TOF/MS) and gas chromatography/mass spectrometry (GC‑MS). Following administration, the metabolisms were detected in the brain, eyes, muscle and gill tissues within 1 h...
December 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27862951/characterization-and-evolution-of-the-spotted-gar-retina
#16
Joshua M Sukeena, Carlos A Galicia, Jacob D Wilson, Tim McGinn, Janette W Boughman, Barrie D Robison, John H Postlethwait, Ingo Braasch, Deborah L Stenkamp, Peter G Fuerst
In this study, we characterize the retina of the spotted gar, Lepisosteus oculatus, a ray-finned fish. Gar did not undergo the whole genome duplication event that occurred at the base of the teleost fish lineage, which includes the model species zebrafish and medaka. The divergence of gars from the teleost lineage and the availability of a high-quality genome sequence make it a uniquely useful species to understand how genome duplication sculpted features of the teleost visual system, including photoreceptor diversity...
November 2016: Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
https://www.readbyqxmd.com/read/27829009/the-morphogenesis-of-cranial-sutures-in-zebrafish
#17
Jolanta M Topczewska, Ramy A Shoela, Joanna P Tomaszewski, Rupa B Mirmira, Arun K Gosain
Using morphological, histological, and TEM analyses of the cranium, we provide a detailed description of bone and suture growth in zebrafish. Based on expression patterns and localization, we identified osteoblasts at different degrees of maturation. Our data confirm that, unlike in humans, zebrafish cranial sutures maintain lifelong patency to sustain skull growth. The cranial vault develops in a coordinated manner resulting in a structure that protects the brain. The zebrafish cranial roof parallels that of higher vertebrates and contains five major bones: one pair of frontal bones, one pair of parietal bones, and the supraoccipital bone...
2016: PloS One
https://www.readbyqxmd.com/read/27826026/chitosan-nanoparticles-and-their-tween-80-modified-counterparts-disrupt-the-developmental-profile-of-zebrafish-embryos
#18
Zhongyue Yuan, Ying Li, Yulan Hu, Jian You, Kazuma Higashisaka, Kazuya Nagano, Yasuo Tsutsumi, Jianqing Gao
Chitosan nanoparticles (CS-NPs) and their Tween 80 modified counterparts (TmCS-NPs) are among the most commonly used brain-targeted vehicles. However, their potential developmental toxicity is poorly understood. In this study, zebrafish embryos are introduced as an in vivo platform. Both NPs showed a dose-dependent increase in developmental toxicity (decreased hatching rate, increased mortality and incidences of malformation). Neurobehavioral changes included decreased spontaneous movement in TmCS-NP treated embryos and hyperactive effect in CS-NP treated larvae...
December 30, 2016: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/27819330/stathmin-like-4-is-critical-for-the-maintenance-of-neural-progenitor-cells-in-dorsal-midbrain-of-zebrafish-larvae
#19
Meng-Ju Lin, Shyh-Jye Lee
A delicate balance between proliferating and differentiating signals is necessary to ensure proper growth and neuronal specification. By studying the developing zebrafish brain, we observed a specific and dynamic expression of a microtubule destabilizer gene, stathmin-like 4 (stmn4), in the dorsal midbrain region. The expression of stmn4 was mutually exclusive to a pan-neuronal marker, elavl3 that indicates its role in regulating neurogenesis. We showed the knockdown or overexpression of stmn4 resulted in premature neuronal differentiation in dorsal midbrain...
November 7, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27811295/spontaneous-alternation-behavior-in-larval-zebrafish
#20
Stefan Yu Bögli, Melody Ying-Yu Huang
Spontaneous alternation behavior (SAB) describes the tendency of animals to alternate their turn direction in consecutive turns. SAB, unlike other mnestic tasks, does not require any prior training or reinforcement. Because of its close correlation with the development and function of the hippocampus in mice, it is thought to reflect a type of memory. Adult zebrafish possess a hippocampus-like structure utilizing the same neurotransmitters as in human brains, and have thus been used to study memory. In the current study, we established SAB in zebrafish larvae at 6 days post-fertilization using a custom-made forced-turn maze with a rate of 57%...
January 15, 2017: Journal of Experimental Biology
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