Debora L Gisch, Michelle Brennan, Blue B Lake, Jeannine Basta, Mark S Keller, Ricardo Melo Ferreira, Shreeram Akilesh, Reetika Ghag, Charles Lu, Ying-Hua Cheng, Kimberly S Collins, Samir V Parikh, Brad H Rovin, Lynn Robbins, Lisa Stout, Kimberly Y Conklin, Dinh Diep, Bo Zhang, Amanda Knoten, Daria Barwinska, Mahla Asghari, Angela R Sabo, Michael J Ferkowicz, Timothy A Sutton, Katherine J Kelly, Ian H De Boer, Sylvia E Rosas, Krzysztof Kiryluk, Jeffrey B Hodgin, Fadhl Alakwaa, Seth Winfree, Nichole Jefferson, Aydın Türkmen, Joseph P Gaut, Nils Gehlenborg, Carrie L Phillips, Tarek M El-Achkar, Pierre C Dagher, Takashi Hato, Kun Zhang, Jonathan Himmelfarb, Matthias Kretzler, Shamim Mollah, Sanjay Jain, Michael Rauchman, Michael T Eadon
There is a need to define regions of gene activation or repression that control human kidney cells in states of health, injury, and repair to understand the molecular pathogenesis of kidney disease and design therapeutic strategies. Comprehensive integration of gene expression with epigenetic features that define regulatory elements remains a significant challenge. We measure dual single nucleus RNA expression and chromatin accessibility, DNA methylation, and H3K27ac, H3K4me1, H3K4me3, and H3K27me3 histone modifications to decipher the chromatin landscape and gene regulation of the kidney in reference and adaptive injury states...
January 10, 2024: Nature Communications