keyword
MENU ▼
Read by QxMD icon Read
search

CID

keyword
https://www.readbyqxmd.com/read/28808998/microfluidics-mass-spectrometry-of-protein-carbohydrate-interactions-applications-to-the-development-of-therapeutics-and-biomarker-discovery
#1
Alina D Zamfir
The functional interactions of carbohydrates and their protein receptors are the basis of biological events critical to the evolution of pathological states. Hence, for the past years, such interactions have become the focus of research for the development of therapeutics and discovery of novel glycan biomarkers based on their binding affinity. Due to the high sensitivity, throughput, reproducibility, and capability to ionize minor species in heterogeneous mixtures, microfluidics-mass spectrometry (MS) has recently emerged as a method of choice in protein-glycan interactomics...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28804477/glaciers-and-ice-sheets-as-analog-environments-of-potentially-habitable-icy-worlds
#2
REVIEW
Eva Garcia-Lopez, Cristina Cid
Icy worlds in the solar system and beyond have attracted a remarkable attention as possible habitats for life. The current consideration about whether life exists beyond Earth is based on our knowledge of life in terrestrial cold environments. On Earth, glaciers and ice sheets have been considered uninhabited for a long time as they seemed too hostile to harbor life. However, these environments are unique biomes dominated by microbial communities which maintain active biochemical routes. Thanks to techniques such as microscopy and more recently DNA sequencing methods, a great biodiversity of prokaryote and eukaryote microorganisms have been discovered...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28803470/effect-of-dmso-on-protein-structure-and-interactions-assessed-by-collision-induced-dissociation-and-unfolding
#3
Daniel S-H Chan, Madeline E Eve Kavanagh, Kirsty J McLean, Andrew William Munro, Dijana Matak-Vinkovic, Anthony G Coyne, Chris Abell
Native mass spectrometry is an ideal technique to investigate the effects of DMSO on protein structure and interac-tions. Given the frequent use of DMSO in biochemical and biophysical assays, it is desirable to understand the influence of DMSO concentration on the dissociation or unfolding behavior of proteins. In this study, the effects of DMSO on the structure and interac-tions of avidin and Mycobacterium tuberculosis (Mtb) CYP142A1 were assessed through collision-induced dissociation (CID) and collision-induced unfolding (CIU) as monitored by nanoelectrospray ionization-ion mobility-mass spectrometry (nESI-IM-MS)...
August 13, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28801822/simultaneous-quantification-of-free-cholesterol-cholesteryl-esters-and-triglycerides-without-ester-hydrolysis-by-uhplc-separation-and-in-source-collision-induced-dissociation-coupled-ms-ms
#4
Michael S Gardner, Lisa G McWilliams, Jeffrey I Jones, Zsuzsanna Kuklenyik, James L Pirkle, John R Barr
We demonstrate the application of in-source nitrogen collision-induced dissociation (CID) that eliminates the need for ester hydrolysis before simultaneous analysis of esterified cholesterol (EC) and triglycerides (TG) along with free cholesterol (FC) from human serum, using normal phase liquid chromatography (LC) coupled to atmospheric pressure chemical ionization (APCI) tandem mass spectrometry (MS/MS). The analysis requires only 50 μL of 1:100 dilute serum with a high-throughput, precipitation/evaporation/extraction protocol in one pot...
August 11, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28801783/screening-of-additives-and-other-chemicals-in-polyurethanes-by-direct-analysis-in-real-time-mass-spectrometry-dart-ms
#5
Ákos Kuki, Lajos Nagy, Tibor Nagy, Miklós Zsuga, Sándor Kéki
Direct analysis in real time mass spectrometry (DART-MS) was used to characterize commercial polyurethane (PUR) samples without sample pretreatment. More than 50 substances, such as catalysts, stabilizers, antioxidants, flame retardants, plasticizers, chain extenders, chain terminators, polyols, solvents, degradation products and contaminants, a few of them presumably toxic, were detected and identified in 18 PUR items. The identification of 16 compounds was further confirmed by DART MS/MS experiments. Catalysts were the largest class of compounds detected in the PURs by DART-MS...
August 11, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28801306/inducible-activation-of-myd88-and-cd40-in-car-t-cells-results-in-controllable-and-potent-antitumor-activity-in-preclinical-solid-tumor-models
#6
Melinda Mata, Claudia Gerken, Phuong Nguyen, Giedre Krenciute, David M Spencer, Stephen Gottschalk
Adoptive immunotherapy with T-cells expressing chimeric antigen receptors (CARs) has had limited success for solid tumors in early phase clinical studies. We reasoned that introducing into CAR T-cells an inducible co-stimulatory (iCO) molecule consisting of a chemical inducer of dimerization (CID)-binding domain and the MyD88 and CD40 signaling domains would improve and control CAR T-cell activation. In the presence of CID, T-cells expressing HER2-CARζ and a MyD88/CD40-based iCO molecule (HER2ζ.iCO T-cells) had superior T-cell proliferation, cytokine production, and ability to sequentially kill targets in vitro relative to HER2ζ...
August 11, 2017: Cancer Discovery
https://www.readbyqxmd.com/read/28800409/cytotoxic-effects-of-the-proton-pump-inhibitor-omeprazole-on-the-non-target-marine-microalga-tetraselmis-suecica
#7
Marta Seoane, Marta Esperanza, Ángeles Cid
Omeprazole (OMP) is one of the most commonly used drugs for the treatment of gastro-intestinal disorders. Although it is daily consumed in high quantities and commonly detected in waters worldwide, relatively little is known about its ecotoxicity. The aim of this study was to evaluate the potential acute toxicity of increasing concentrations of OMP on the marine microalga Tetraselmis suecica analysing several cytotoxicity biomarkers by flow cytometry after 24h of exposure. Results showed that OMP caused a decrease in growth and autofluorescence, an increase in cellular volume and intracellular complexity, hyperpolarization of cytoplasmic and mitochondrial membranes and intracellular acidification...
August 2, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28797762/ceramide-enhances-cox-2-expression-and-vsmc-contractile-hyperreactivity-via-er-stress-signal-activation
#8
Huina Zhang, Juanfen Li, Linghai Li, Pingsheng Liu, Yongxiang Wei, Zongjie Qian
Ceramide accumulation in blood vessels has been attributed to vascular dysfunction in progressive vascular complications in metabolic diseases. The present study showed that ceramide pretreatment promoted PE-induced vasoconstriction in rat endothelium-denuded vascular rings in a time- and dose-dependent manner. Endoplasmic reticulum (ER) stress inhibitors, 4-PBA and TUDCA, COX-2 inhibitors, Celecoxib and NS398, as well as PGE2 receptor antagonist AH-6809 attenuated ceramide-promoted vascular hyperreactivity...
August 7, 2017: Vascular Pharmacology
https://www.readbyqxmd.com/read/28797641/b2-kinin-receptors-in-the-dorsal-periaqueductal-gray-are-implicated-in-the-panicolytic-like-effect-of-opiorphin
#9
Caio César Sestile, Jhonatan Christian Maraschin, Marcel Pereira Rangel, Rosangela Getirana Santana, Hélio Zangrossi, Frederico Guilherme Graeff, Elisabeth Aparecida Audi
Reported results have shown that the pentapeptide opiorphin inhibits oligopeptidases that degrade brain neuropeptides, and has analgesic and antidepressant effects in experimental animals, without either tolerance or dependency after chronic administration. In a previous study we showed that opiorphin has a panicolytic-like effect in the dorsal periaqueductal gray (dPAG) electrical stimulation test (EST), mediated by the μ-opioid receptor (MOR). This study further analyzes the mechanism of opiorphin panicolytic action, using the EST and drug injection inside the dPAG...
August 7, 2017: Progress in Neuro-psychopharmacology & Biological Psychiatry
https://www.readbyqxmd.com/read/28793009/nonylphenol-properties-legislation-toxicity-and-determination
#10
Frederico G DE Araujo, Glauco F Bauerfeldt, Yara Peluso Cid
This paper aims to gather and discuss important information about nonylphenol, such as physical chemistry properties, toxicity and analytical methods in various matrices. As a degradation product of ethoxylated alkylphenols, nonylphenol presents a higher degree of reactivity than its precursor. Due to its harmful effects on the environment, use and production of nonylphenol has been banned in European Union countries, alongside their precursors. The guide on quality of drinking water (USEPA) recommends a maximum concentration of 28 µg L-1 for fresh water...
August 7, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/28789846/additive-value-of-the-crusade-score-to-the-grace-score-for-mortality-risk-prediction-in-patients-with-acute-coronary-syndromes
#11
Alberto Cordero, Moisés Rodriguez-Manero, Jose M García-Acuña, Ramón López-Palop, Belen Cid, Pilar Carrillo, Rosa Agra-Bermejo, Violeta González-Salvado, Diego Iglesias-Alvarez, Vicente Bertomeu-Martínez, Jose R González-Juanatey
INTRODUCTION: Acute coronary syndrome (ACS) treatments increase bleeding complications that also impair prognosis. Bleeding risk scores reclassification of actual mortality risk estimated by the GRACE score might improve overall estimation. METHODS: Observational and prospective study of all ACS patients admitted in two hospitals. Mortality risk was assessed by the GRACE score and bleeding risk by the CRUSADE score. We analyzed the net reclassification improvement (NRI) of adding the CRUSADE score to the GRACE score...
August 2, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/28783911/wind-induced-changes-in-the-dynamics-of-fluorescent-organic-matter-in-the-coastal-nw-mediterranean
#12
Fran L Aparicio, Mar Nieto-Cid, Eva Calvo, Carles Pelejero, Àngel López-Sanz, Josep Pascual, Jordi Salat, Elvia D Sánchez-Pérez, Patricia De La Fuente, Josep M Gasol, Cèlia Marrasé
Marine biogeochemistry dynamics in coastal marine areas is strongly influenced by episodic events such as rain, intense winds, river discharges and anthropogenic activities. We evaluated in this study the importance of these forcing events on modulating seasonal changes in the marine biogeochemistry of the northwestern coast of the Mediterranean Sea, based on data gathered from a fixed coastal sampling station in the area. A 4-year (2011-2014) monthly sampling at four depths (0.5m, 20m, 50m and 80m) was performed to examine the time variability of several oceanographic variables: seawater temperature, salinity, inorganic nutrient concentrations (NO3(-), PO4(3-) and SiO2), chlorophyll a (Chl a), dissolved organic carbon (DOC) and fluorescent dissolved organic matter (FDOM)...
August 1, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28783691/characterization-of-t-and-b-cell-repertoire-diversity-in-patients-with-rag-deficiency
#13
Yu Nee Lee, Francesco Frugoni, Kerry Dobbs, Irit Tirosh, Likun Du, Francesca A Ververs, Heng Ru, Lisa Ott de Bruin, Mehdi Adeli, Jacob H Bleesing, David Buchbinder, Manish J Butte, Caterina Cancrini, Karin Chen, Sharon Choo, Reem A Elfeky, Andrea Finocchi, Ramsay L Fuleihan, Andrew R Gennery, Dalia H El-Ghoneimy, Lauren A Henderson, Waleed Al-Herz, Elham Hossny, Robert P Nelson, Sung-Yun Pai, Niraj C Patel, Shereen M Reda, Pere Soler-Palacin, Raz Somech, Paolo Palma, Hao Wu, Silvia Giliani, Jolan E Walter, Luigi D Notarangelo
Recombination-activating genes 1 and 2 (RAG1 and RAG2) play a critical role in T and B cell development by initiating the recombination process that controls the expression of T cell receptor (TCR) and immunoglobulin genes. Mutations in the RAG1 and RAG2 genes in humans cause a broad spectrum of phenotypes, including severe combined immunodeficiency (SCID) with lack of T and B cells, Omenn syndrome, leaky SCID, and combined immunodeficiency with granulomas or autoimmunity (CID-G/AI). Using next-generation sequencing, we analyzed the TCR and B cell receptor (BCR) repertoire in 12 patients with RAG mutations presenting with Omenn syndrome (n = 5), leaky SCID (n = 3), or CID-G/AI (n = 4)...
December 16, 2016: Science Immunology
https://www.readbyqxmd.com/read/28765495/validating-reference-equations-for-impulse-oscillometry-in-healthy-mexican-children
#14
Laura Gochicoa-Rangel, Rodrigo Del Río-Hidalgo, Juana Hernández-Ruiz, Luis Rodríguez-Moreno, David Martínez-Briseño, Uri Mora-Romero, Silvia Cid-Juárez, Cecilia García-Sancho, Luis Torre-Bouscoulet
BACKGROUND: The impulse oscillometry system (IOS) measures the impedance (Z) of the respiratory system, but proper interpretation of its results requires adequate reference values. The objectives of this work were: (1) to validate the reference equations for the IOS published previously by our group and (2) to compare the adjustment of new available reference equations for the IOS from different countries in a sample of healthy children. METHODS: Subjects were healthy 4-15-y-old children from the metropolitan area of Mexico City, who performed an IOS test...
August 1, 2017: Respiratory Care
https://www.readbyqxmd.com/read/28764333/trimethylation-enhancement-using-13-c-diazomethane-gas-phase-charge-inversion-of-modified-phospholipid-cations-for-enhanced-structural-characterization
#15
Stella K Betancourt, Carlos R Canez, Samuel W J Shields, Jeffrey M Manthorpe, Jeffrey C Smith, Scott A McLuckey
Methylation of phospholipids (PL) leads to increased uniformity in positive electrospray ionization (ESI) efficiencies across the various PL subclasses. This effect is realized in the approach referred to as "trimethylation enhancement using (13)C-diazomethane" ((13)C-TrEnDi), which results in the methyl esterification of all acidic sites and the conversion of amines to quaternary ammonium sites. Collision-induced dissociation (CID) of these cationic modified lipids enables class identification by forming distinctive headgroup fragments based on the number of (13)C atoms incorporated during derivatization...
August 15, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28761929/validation-of-the-xdp-mdsp-rating-scale-for-the-evaluation-of-patients-with-x-linked-dystonia-parkinsonism
#16
Paul Matthew D Pasco, Roland Dominic G Jamora, Raymond L Rosales, Cid Czarina E Diesta, Arlene R Ng, Rosalia A Teleg, Criscely L Go, Lillian Lee, Hubert H Fernandez
X-linked dystonia-parkinsonism(XDP) is a neurodegenerative disorder endemic to the Philippines. A rating scale was developed by the authors under the guidance of the Movement Disorder Society of the Philippines (MDSP) to assess XDP severity and progression, functional impact, and response to treatment in future clinical trials. Our main objective was to validate our new scale, the XDP-MDSP scale. The initial validation process included pragmatic testing to XDP patients followed by a modified Delphi procedure with an international advisory panel of dystonia, parkinsonism and scale development experts...
2017: NPJ Parkinson's Disease
https://www.readbyqxmd.com/read/28760923/teaching-neuroimages-bilateral-intracerebral-hemorrhage-in-expanded-dengue-syndrome
#17
Wagner Cid Palmeira Cavalcante, Herval Ribeiro Soares Neto, Ronnyson Susano Grativvol, Raphael Ribeiro Spera, Fernando Pereira Frassetto, Leandro Tavares Lucato, Ricardo Nitrini
No abstract text is available yet for this article.
August 1, 2017: Neurology
https://www.readbyqxmd.com/read/28754746/tumor-derived-exosomes-modulate-pd-l1-expression-in-monocytes
#18
Franziska Haderk, Ralph Schulz, Murat Iskar, Laura Llaó Cid, Thomas Worst, Karolin V Willmund, Angela Schulz, Uwe Warnken, Jana Seiler, Axel Benner, Michelle Nessling, Thorsten Zenz, Maria Göbel, Jan Dürig, Sven Diederichs, Jérôme Paggetti, Etienne Moussay, Stephan Stilgenbauer, Marc Zapatka, Peter Lichter, Martina Seiffert
In chronic lymphocytic leukemia (CLL), monocytes and macrophages are skewed toward protumorigenic phenotypes, including the release of tumor-supportive cytokines and the expression of immunosuppressive molecules such as programmed cell death 1 ligand 1 (PD-L1). To understand the mechanism driving protumorigenic skewing in CLL, we evaluated the role of tumor cell-derived exosomes in the cross-talk with monocytes. We carried out RNA sequencing and proteome analyses of CLL-derived exosomes and identified noncoding Y RNA hY4 as a highly abundant RNA species that is enriched in exosomes from plasma of CLL patients compared with healthy donor samples...
July 28, 2017: Science Immunology
https://www.readbyqxmd.com/read/28753276/sequencing-and-identification-of-endogenous-neuropeptides-with-matrix-enhanced-secondary-ion-mass-spectrometry-tandem-mass-spectrometry
#19
Nina Ogrinc Potočnik, Gregory L Fisher, Arnoud Prop, Ron M A Heeren
Matrix-enhanced secondary ion mass spectrometry (ME-SIMS) has overcome one of the biggest disadvantages of SIMS analysis by providing the ability to detect intact biomolecules at high spatial resolution. By increasing ionization efficiency and minimizing primary ion beam-induced fragmentation of analytes, ME-SIMS has proven useful for detection of numerous biorelevant species, now including peptides. We report here the first demonstration of tandem ME-SIMS for de novo sequencing of endogenous neuropeptides from tissue in situ (i...
August 3, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28748995/-effects-of-a-mindfulness-intervention-in-chilean-high-schoolers
#20
Álvaro I Langer, Carlos Schmidt, José Manuel Aguilar-Parra, Cristian Cid, Antonella Magni
BACKGROUND: Mindfulness has been conceptualized as paying attention to present moment experience in a non-judgmental manner, and the practice of developing that skill. AIM: To determine the impact of a mindfulness-based intervention on negative emotional states of anxiety, stress, and depression in Chilean high schoolers. MATERIAL AND METHODS: Eighty-eight teenagers aged 13 ± 0.6 years (46 females) were randomly assigned to a mindfulness group or a control (41 and 47, respectively)...
April 2017: Revista Médica de Chile
keyword
keyword
75640
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"