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Praveen Krishna Veerasubramanian, Ponrasu Thangavel, Ramya Kannan, Sudip Chakraborty, Balaji Ramachandran, Lonchin Suguna, Vignesh Muthuvijayan
We have developed a novel hydrogel composed of konjac glucomannan (KGM), human hair proteins (KER), and an ethanolic extract of Avena sativa (OAT) and evaluated its potential as a dressing material for diabetic wounds. KGM is an excellent biocompatible gelling agent that stimulates fibroblast proliferation and immunomodulation. Human hair proteins (KER) are biocompatible, biodegradable, and possess abundant cell adhesion sites. KER also promotes fibroblast attachment and proliferation, keratinocyte migration, and collagen expression, which can accelerate wound healing...
February 13, 2018: Colloids and Surfaces. B, Biointerfaces
Karine Modolon Zepon, Morgana Souza Marques, Marcos Marques da Silva Paula, Fernando Dal Pont Morisso, Luiz Alberto Kanis
This manuscript was focused on introducing a facile, green and scalable method to produce kappa-carrageenan (κC) hydrogel membranes containing in situ synthesized silver nanoparticles (AgNPs). In a typical protocol, κC hydrogels were obtained by heating (sol phase), followed by cooling (gel phase) the polysaccharide solution, which enabled the simultaneous synthesis of AgNPs during the heating time. The as synthesized AgNPs were characterized spectrophotometrically, and by dynamic light scattering and transmission electron microscopy...
February 19, 2018: International Journal of Biological Macromolecules
Manuel Gregoritza, Kathrin Abstiens, Moritz Graf, Achim M Goepferich
Reducing burst effects, providing controlled release, and safeguarding biologics against degradation are a few of several highly attractive applications for microgels in the field of controlled release. However, the incorporation of proteins into microgels without impairing stability is highly challenging. In this proof of concept study, the combination of microfluidics and thiol-ene photoclick chemistry was evaluated for the fabrication of antibody-loaded microgels with narrow size distribution. Norbornene-modified eight-armed poly(ethylene glycol) with an average molecular mass of 10,000 Da, 20,000 Da, or 40,000 Da were prepared as macromonomers for microgel formation...
February 19, 2018: European Journal of Pharmaceutics and Biopharmaceutics
Maria Lucia Frade, Sarah Raquel de Annunzio, Giovana Maria Fioramonti Calixto, Francesca Damiani Victorelli, Marlus Chorilli, Carla Raquel Fontana
Chitosan (CH) is a biopolymer that exhibits a number of interesting properties such as anti-inflammatory and antibacterial activity and is also a promising platform for the incorporation of photosensitizing agents. This study aimed to evaluate the efficacy of antimicrobial activity of chitosan hydrogel formulation alone and in combination with the methylene blue (MB) associated with antimicrobial photodynamic therapy (aPDT) against planktonic and biofilm phase of Propionibacterium acnes . Suspensions were sensitized with 12...
February 22, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Jiqing Yang, Xiaotong Hu, Jia Xu, Xin Liu, Li Yang
A novel capillary electrophoresis-integrated immobilized enzyme reactor (CE-integrated IMER) is developed using single-step in-situ acetylcholinesterase (AChE)-mediated alginate hydrogelation and enzyme encapsulation. Alginate hydrogelation with "egg-box" structure is triggered inside a capillary with releasing of Ca2+ by changing the pH of the sol solution, which is accomplished in-situ by AChE-catalyzed hydrolysis reaction of acetylthiocholine to produce acetic acid. AChE and any other enzyme initially contained in the sol solution [e...
February 22, 2018: Analytical Chemistry
L Datko Williams, A Farley, M Cupelli, S Alapati, M S Kennedy, D Dean
Dental pulp stromal cells (DPSCs) can be differentiated down lineages known to either express bone or dentin specific protein markers. . Since the differentiation of cells can be heavily influenced by their environment, it may be possible to influence the osteogenic/odontogenic potential of DPSCs by modulating the mechanical properties of substrate on which they are grown. In this study, human DPSCs were grown with and without hydroxyapatite (HA) microparticles on a range of substrates including fibronectin-coated hydrogels and glass substrates, which represented an elastic moduli range of ∼ 3 kPa - 50 GPa, over a 21 day period...
February 22, 2018: Journal of Biomedical Materials Research. Part A
Akshay A Narkhede, James H Crenshaw, Riley M Manning, Shreyas S Rao
Breast cancer brain metastasis marks the most advanced stage of breast cancer no longer considered curable with a median survival period of ∼ 4-16 months. Apart from the genetic susceptibility (subtype) of breast tumors, brain metastasis is also dictated by the biophysical/chemical interactions of tumor cells with native brain microenvironment, which remain obscure, primarily due to the lack of tunable biomimetic in vitro models. To address this need, we utilized a biomimetic hyaluronic acid (HA) hydrogel platform to elucidate the impact of matrix stiffness on the behavior of MDA-MB-231Br cells, a brain metastasizing variant of the triple negative breast cancer line MDA-MB-231...
February 22, 2018: Journal of Biomedical Materials Research. Part A
Mirele S Vasconcelos, Tamiris F G Souza, Ingrid S Figueiredo, Emília T Sousa, Felipe D Sousa, Renato A Moreira, Nylane M N Alencar, José V Lima-Filho, Márcio V Ramos
The healing performance of a hydrogel composed of hemicelluloses extracted from seeds of Caesalpinia pulcherrima (Fabaceae) and mixed with phytomodulatory proteins obtained from the latex of Calotropis procera was characterized on excisional wounds. The hydrogel did not induce dermal irritability. When topically used on excisional wounds, the hydrogel enhanced healing by wound contraction. Histology and the measurement of inflammatory mediators (myeloperoxidase, interleukin-1β, and interleukin-6) suggested that the inflammatory phase of the healing process was intensified, stimulating fibroplasia and neovascularization (proliferative phase) and tissue remodeling by increasing new collagen fiber deposition...
February 22, 2018: Phytotherapy Research: PTR
Noam Cohen, Einat Toister, Yonatan Lati, Meni Girshengorn, Lilach Levin, Lea Silberstein, Dror Seliktar, Eyal Epstein
Recent advances in the bioengineering field have introduced new opportunities enabling cell encapsulation in three-dimensional (3D) structures using either various natural or synthetic materials. However, such hydrogel scaffolds have not been fully biocompatible for cell cultivation due to the lack of physical stability or bioactivity. Here, we utilized a uniquely fabricated semi-synthetic 3D polyethylene glycol-fibrinogen (PEG-Fb) hydrogel scaffold, which exhibits both high stability and high bioactivity, to encapsulate HEK293 cells for the production of human recombinant acetylcholine esterase (AChE)...
February 21, 2018: Cytotechnology
Liping Sun, Yong Zhong, Jie Gui, Xianwu Wang, Xiaorong Zhuang, Jian Weng
Background: Alzheimer's disease (AD) is a neurodegenerative disorder characterized by progressive cognitive and memory impairment. It is the most common neurological disease that causes dementia. Soluble amyloid-beta oligomers (AβO) in blood or cerebrospinal fluid (CSF) are the pathogenic biomarker correlated with AD. Methods: A simple electrochemical biosensor using graphene oxide/gold nanoparticles (GNPs) hydrogel electrode was developed in this study. Thiolated cellular prion protein (PrPC ) peptide probe was immobilized on GNPs of the hydrogel electrode to construct an AβO biosensor...
2018: International Journal of Nanomedicine
Chao Wang, Jinqiang Wang, Xudong Zhang, Shuangjiang Yu, Di Wen, Quanyin Hu, Yanqi Ye, Hunter Bomba, Xiuli Hu, Zhuang Liu, Gianpietro Dotti, Zhen Gu
Patients with low-immunogenic tumors respond poorly to immune checkpoint blockade (ICB) targeting the programmed death-1 (PD-1)/programmed death-ligand 1 (PD-L1) pathway. Conversely, patients responding to ICB can experience various side effects. We have thus engineered a therapeutic scaffold that, when formed in situ, allows the local release of gemcitabine (GEM) and an anti-PD-L1 blocking antibody (aPDL1) with distinct release kinetics. The scaffold consists of reactive oxygen species (ROS)-degradable hydrogel that releases therapeutics in a programmed manner within the tumor microenvironment (TME), which contains abundant ROS...
February 21, 2018: Science Translational Medicine
Alexia Vite, Caimei Zhang, Roslyn Yi, Sabrina Emms, Glenn L Radice
Shortly after birth, muscle cells of the mammalian heart lose their ability to divide. At the same time, the N-cadherin/catenin cell adhesion complex accumulates at the cell termini creating a specialized type of cell-cell contact called the intercalated disc (ICD). To investigate the relationship between ICD maturation and proliferation, αE-catenin ( Ctnna1 ) and αT-catenin ( Ctnna3 ) genes were deleted to generate cardiac-specific α-catenin double knockout (DKO) mice. DKO mice exhibited aberrant N-cadherin expression, mislocalized actomyosin activity, and increased cardiomyocyte proliferation that was dependent on Yap activity...
February 21, 2018: Development
Qi Zhu, Xietao Yuan, Yihao Zhu, Jiangfeng Ni, Xiaohua Zhang, Zhaohui Yang
In this paper we fabricate a robust flexible solid-state supercapacitor (FSC) device by embedding a conductive poly (vinyl alcohol) hydrogel into the ACNT arrays. We carefully investigate the distribution, interface and densification effect on the electrochemical properties of CNTs in the gel matrix. A total electrochemical capacitance of the device is measured to be 227 mF·cm-3 with the maximum energy density of 0.02 mW·h·cm-3, which is dramatically enhanced compared to the similar device composed of unparallel CNTs...
February 21, 2018: Nanotechnology
Zheng Zhang, Chen Liu, Chunzheng Yang, Yuyang Wu, Feng Yu, Yong Chen, Jie Du
Various hydrogels have been used as proteins delivery in the treatment of human diseases. Nevertheless, it is always difficult to control capture and release of multiple proteins in different regions and periods. This research successfully proves that multiple proteins can be captured and released from the aptamer-patterned hydrogel films with adjustable release rate at prospective time and in specific regions utilizing the complementary DNA strand (cDNA) of aptamer via photoclick chemistry and DNA hybridization...
February 21, 2018: ACS Applied Materials & Interfaces
Xiaohu Zhou, Tianzhen Li, Jiahui Wang, Fan Chen, Dan Zhou, Qi Liu, Baijia Li, Jingyue Cheng, Xuechang Zhou, Bo Zheng
Stimuli-responsive hydrogels that undergo programmable shape deformation are of great importance for a wide variety of applications spanning from soft robotics and biomedical devices to tissue engineering and drug delivery. To guide the shape morphing, anisotropic elements need to be encoded into the hydrogels during the fabrication yet extremely difficult to alter afterward. This study reports a simple and reliable mechano-chemical regulation strategy to post-engineer the hydrogels by encoding structures of high stiffness locally into pre-stretched tough hydrogels through ion transfer printing with a paper cut...
February 21, 2018: ACS Applied Materials & Interfaces
C R Carvalho, S Wrobel, C Meyer, C Brandenberger, I F Cengiz, R López-Cebral, J Silva-Correia, G Ronchi, R L Reis, C Grothe, J M Oliveira, K Haastert-Talini
Peripheral nerve injuries (PNI) resulting in a gap to be bridged between the transected nerve ends are commonly reconstructed with autologous nerve tissue, but there is a need for valuable alternatives. This experimental work considers the innovative use of the biomaterial Gellan Gum (GG) as a luminal filler for nerve guidance channels made from chitosan with a 5% degree of acetylation. The engineered constructs should remodel the structural support given to regenerating axons by the so-called bands of Büngner...
February 21, 2018: Biomaterials Science
Zelong Chen, Chenggong Yan, Shina Yan, Qin Liu, Meirong Hou, Yikai Xu, Rui Guo
Numerous biodegradable hydrogels for cartilage regeneration have been widely used in the field of tissue engineering. However, to non-invasively monitor hydrogel degradation and efficiently evaluate cartilage restoration in situ is still challenging. Methods: A ultrasmall superparamagnetic iron oxide (USPIO)-labeled cellulose nanocrystal (CNC)/silk fibroin (SF)-blended hydrogel system was developed to monitor hydrogel degradation during cartilage regeneration. The physicochemical characterization and biocompatibility of the hydrogel were evaluated in vitro ...
2018: Theranostics
Ruixiang Li, Jianming Liang, Yuwei He, Jing Qin, Huining He, Seungjin Lee, Zhiqing Pang, Jianxin Wang
The outcome of scaffold-based stem cell transplantation remains unsatisfied due to the poor survival of transplanted cells. One of the major hurdles associated with the stem cell survival is the immune rejection, which can be effectively reduced by the use of immunosuppressant. However, ideal localized and sustained release of immunosuppressant is difficult to be realized, because it is arduous to hold the drug delivery system within scaffold for a long period of time. In the present study, the sustained release of immunosuppressant for the purpose of improving the survival of stem cells was successfully realized by a nanoparticle-anchoring hydrogel scaffold we developed...
2018: Theranostics
Rasheed Ahmad, Yan Deng, Ravina Singh, Mubashir Hussain, Muhammad Ali Abdullah Shah, Sauli Elingarami, Nongyue He, Yueming Sun
Materials derived from biological sources not only offer biocompatibility but also adjust with the disease for elongated treatments and more effective therapies. These materials can be utilized as building blocks to construct state of the art drug delivery vehicles like nanoparticles, hydrogels, and nanofibers capable of dramatically enhancing the therapeutic efficiency in cancer treatment. New emerging trends in drug delivery design are constantly reported in recent literature using carbohydrates like cellulose, chitosan, and alginate and proteins like albumin, collagen, gelatin, and zein...
January 1, 2018: Journal of Biomedical Nanotechnology
Victoria Horbert, Long Xin, Peter Foehr, Olaf Brinkmann, Matthias Bungartz, Rainer H Burgkart, T Graeve, Raimund W Kinne
Objective Limitations of matrix-assisted autologous chondrocyte implantation to regenerate functional hyaline cartilage demand a better understanding of the underlying cellular/molecular processes. Thus, the regenerative capacity of a clinically approved hydrogel collagen type I implant was tested in a standardized bovine cartilage punch model. Methods Cartilage rings (outer diameter 6 mm; inner defect diameter 2 mm) were prepared from the bovine trochlear groove. Collagen implants (± bovine chondrocytes) were placed inside the cartilage rings and cultured up to 12 weeks...
February 1, 2018: Cartilage
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