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https://www.readbyqxmd.com/read/28346796/harnessing-external-cues-development-and-evaluation-of-an-in-vitro-culture-system-for-osteochondral-tissue-engineering
#1
Deborah L Dorcemus, Eve O George, Caroline N Dealy, Syam P Nukavarapu
Over the last decade, engineered structures have been developed for osteochondral (OC) tissue regeneration. While the optimal structure design is yet to be determined, these scaffolds require in vitro evaluation before clinical use. However, the means by which complex scaffolds, such as OC scaffolds, can be tested are limited. Taking advantage of a mesenchymal stem cell's (MSC's) ability to respond to its surrounding we harness external cues, such as the cell's mechanical environment and delivered factors, to create an in vitro culture system for OC tissue engineering with a single cell source on a gradient yet integrated scaffold system...
March 24, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28346376/blend-hydrogel-microspheres-of-carboxymethyl-chitosan-and-gelatin-for-the-controlled-release-of-5-fluorouracil
#2
Vanarchi Rajini Kanth, Praveen B Kajjari, Priya M Madalageri, Sakey Ravindra, Lata S Manjeshwar, Tejraj M Aminabhavi
Carboxymethyl chitosan (CMCS) was synthesized and blended with gelatin (GE) to prepare hydrogel microspheres by w/o emulsion cross-linking in the presence of glutaraldehyde (GA), which acted as a cross-linker. 5-Fluorouracil (5-FU) was encapsulated to investigate its controlled release (CR) characteristics in acidic (pH 1.2) and alkaline (pH 7.4) buffer media. The microspheres which formed were spherical in nature, with smooth surfaces, as judged by the scanning electron microscopy (SEM). Fourier transform infrared spectroscopy (FTIR) confirmed the carboxymethylation of CS and the chemical stability of 5-FU in the formulations...
March 27, 2017: Pharmaceutics
https://www.readbyqxmd.com/read/28346104/bioengineered-3d-glial-cell-culture-systems-and-applications-for-neurodegeneration-and-neuroinflammation
#3
P Marc D Watson, Edel Kavanagh, Gary Allenby, Matthew Vassey
Neurodegeneration and neuroinflammation are key features in a range of chronic central nervous system (CNS) diseases such as Alzheimer's and Parkinson's disease, as well as acute conditions like stroke and traumatic brain injury, for which there remains significant unmet clinical need. It is now well recognized that current cell culture methodologies are limited in their ability to recapitulate the cellular environment that is present in vivo, and there is a growing body of evidence to show that three-dimensional (3D) culture systems represent a more physiologically accurate model than traditional two-dimensional (2D) cultures...
February 1, 2017: SLAS Discov
https://www.readbyqxmd.com/read/28345876/engineering-cellular-microenvironments-with-photo-and-enzymatically-responsive-hydrogels-toward-biomimetic-3d-cell-culture-models
#4
Roger Y Tam, Laura J Smith, Molly S Shoichet
Conventional cell culture techniques using 2D polystyrene or glass have provided great insight into key biochemical mechanisms responsible for cellular events such as cell proliferation, differentiation, and cell-cell interactions. However, the physical and chemical properties of 2D culture in vitro are dramatically different than those found in the native cellular microenvironment in vivo. Cells grown on 2D substrates differ significantly from those grown in vivo, and this explains, in part, why many promising drug candidates discovered through in vitro drug screening assays fail when they are translated to in vivo animal or human models...
March 27, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28345866/biocompatible-hydroxylated-boron-nitride-nanosheets-polyvinyl-alcohol-interpenetrating-hydrogels-with-enhanced-mechanical-and-thermal-responses
#5
Lin Jing, Hongling Li, Roland Yingjie Tay, Bo Sun, Siu Hon Tsang, Olivier Cometto, Jinjun Lin, Edwin Hang Tong Teo, Alfred Iing Yoong Tok
Polyvinyl alcohol (PVA) hydrogels with tissue-like viscoelasticity, excellent biocompatibility and high hydrophilicity have been considered as promising cartilage replacement materials. However, lack of sufficient mechanical properties is a critical barrier to their usage as load-bearing cartilage substitutes. Herein, we report hydroxylated boron nitride nanosheets (OH-BNNS)/PVA interpenetrating hydrogels by cyclically freezing/thawing the aqueous mixture of PVA and highly hydrophilic OH-BNNS (up to 0.6 mg/mL, two times the highest reported so far)...
March 27, 2017: ACS Nano
https://www.readbyqxmd.com/read/28345591/activation-of-the-mechanosensitive-ion-channel-mscl-by-mechanical-stimulation-of-supported-droplet-hydrogel-bilayers
#6
Kadla R Rosholm, Matthew A B Baker, Pietro Ridone, Yoshitaka Nakayama, Paul R Rohde, Luis G Cuello, Lawrence K Lee, Boris Martinac
The droplet on hydrogel bilayer (DHB) is a novel platform for investigating the function of ion channels. Advantages of this setup include tight control of all bilayer components, which is compelling for the investigation of mechanosensitive (MS) ion channels, since they are highly sensitive to their lipid environment. However, the activation of MS ion channels in planar supported lipid bilayers, such as the DHB, has not yet been established. Here we present the activation of the large conductance MS channel of E...
March 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28345415/chondrogenic-gene-expression-differences-between-chondrocytes-from-osteoarthritic-and-non-oa-trauma-joints-in-a-3d-collagen-type-i-hydrogel
#7
Vivek Jeyakumar, Florian Halbwirth, Eugenia Niculescu-Morzsa, Christoph Bauer, Hannes Zwickl, Daniela Kern, Stefan Nehrer
Objective The purpose of the current study was to compare the donor age variation of chondrocytes from non-OA (osteoarthritic) trauma joints in patients of young to middle age (20.5 ± 3.7, 31.8 ± 1.9, 41.9 ± 4.1 years) embedded in matrix-associated autologous chondrocyte transplantation (MACT) grafts (CaReS). The chondrocyte-specific gene expression of CaReS grafts were then compared to chondrocytes from OA joints (in patients aged 63.8 ± 10 years) embedded in a collagen type I hydrogel. Design OA chondrocytes and articular chondrocyte-laden grafts were cultured over 14 days in chondrogenic growth medium...
April 2017: Cartilage
https://www.readbyqxmd.com/read/28345345/continuously-electrotriggered-core-coalescence-of-double-emulsion-drops-for-microreactions
#8
Likai Hou, Yukun Ren, Yankai Jia, Xiaokang Deng, Weiyu Liu, Xiangsong Feng, Hongyuan Jiang
Microfluidically-generated double emulsions are promising templates for microreactions, which protect the reaction from external disturbance and enable in vitro analyses with large-scale samples. Controlled combination of their inner droplets in a continuous manner is an essential requirement toward truly applications. Here, we first generate dual-cored double-emulsion drops with different inner encapsulants using a capillary microfluidic device; then, we transfer the emulsion drops into another electrode integrated PDMS microfluidic device and utilize external AC electric field to continuously trigger the coalescence of inner cores inside these emulsion drops in continuous flow...
March 27, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28345045/bend-stretch-and-touch-locating-a-finger-on-an-actively-deformed-transparent-sensor-array
#9
Mirza Saquib Sarwar, Yuta Dobashi, Claire Preston, Justin K M Wyss, Shahriar Mirabbasi, John David Wyndham Madden
The development of bendable, stretchable, and transparent touch sensors is an emerging technological goal in a variety of fields, including electronic skin, wearables, and flexible handheld devices. Although transparent tactile sensors based on metal mesh, carbon nanotubes, and silver nanowires demonstrate operation in bent configurations, we present a technology that extends the operation modes to the sensing of finger proximity including light touch during active bending and even stretching. This is accomplished using stretchable and ionically conductive hydrogel electrodes, which project electric field above the sensor to couple with and sense a finger...
March 2017: Science Advances
https://www.readbyqxmd.com/read/28345030/wearable-disposable-sweat-based-glucose-monitoring-device-with-multistage-transdermal-drug-delivery-module
#10
Hyunjae Lee, Changyeong Song, Yong Seok Hong, Min Sung Kim, Hye Rim Cho, Taegyu Kang, Kwangsoo Shin, Seung Hong Choi, Taeghwan Hyeon, Dae-Hyeong Kim
Electrochemical analysis of sweat using soft bioelectronics on human skin provides a new route for noninvasive glucose monitoring without painful blood collection. However, sweat-based glucose sensing still faces many challenges, such as difficulty in sweat collection, activity variation of glucose oxidase due to lactic acid secretion and ambient temperature changes, and delamination of the enzyme when exposed to mechanical friction and skin deformation. Precise point-of-care therapy in response to the measured glucose levels is still very challenging...
March 2017: Science Advances
https://www.readbyqxmd.com/read/28344094/controlled-release-of-antibiotic-amoxicillin-drug-using-carboxymethyl-cellulose-cl-poly-itaconic-acid-co-lactic-acid-hydrogel
#11
S Sood, Vinod Kumar Gupta, Shilpi Agarwal, Kamal Dev, Deepak Pathania
In this paper, microwave assisted preparation of carboxymethyl cellulose-cl-poly(lactic acid-co-itaconic acid) (CMC-cl-P(LA-co-IA)) hydrogel was reported via facile graft copolymerization using N, N(1)-methylene-bis-acrylamide (MBA) and potassium persulphate as cross linker and initiator. Different reaction parameters were optimized to achieve good yield. The formation of hydrogel was confirmed by characterization techniques such as Fourier transform infrared spectroscopy, field emission scanning electron microscopy, X-ray diffraction, thermo gravimetric analysis, transmission electron microscopy etc...
March 23, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28344093/synthesis-and-characterization-of-a-novel-cationic-hydrogel-base-on-salecan-g-pmaptac
#12
Wei Wei, Xiaoliang Qi, Junjian Li, Yin Zhong, Gancheng Zuo, Xihao Pan, Ting Su, Jianfa Zhang, Wei Dong
Salecan is a biological macromolecular and biocompatible polysaccharide that have been investigated for recent years. Herein, we report a novel cationic hydrogel fabricated by graft-polymerizing 3-(methacryloylamino)propyl-trimethylammonium chloride (MAPTAC) onto salecan chains. The obtained hydrogels were transparent, solid-elastic, macro-porous, ion-sensitive, and non-cytotoxic. The swelling ratios increased with salecan content, while mechanical strength does the opposite. Moreover, drug delivery test was studied as a potential application...
March 23, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28344018/enhanced-topical-penetration-system-exposure-and-anti-psoriasis-activity-of-two-particle-sized-curcumin-loaded-plga-nanoparticles-in-hydrogel
#13
Lin Sun, Zeyu Liu, Lun Wang, Dongmei Cun, Henry H Y Tong, Ru Yan, Xin Chen, Ruibing Wang, Ying Zheng
Psoriasis is an immune-mediated skin disorder, which is triggered by the aberrant activation of dendritic cells in skin. This activation is followed by the complex interaction between the immune cells in the skin and keratinocyte in the epidermis. To improve the conditions of poor aqueous solubility and chemical stability, overcome skin barriers, and enhance in-vivo anti-psoriatic activity, curcumin (Cur) loaded poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) were fabricated and administered by topical route to treat Imiquimod (IMQ)-induced psoriasis-like mouse model...
March 23, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28343999/a-genome-wide-analysis-of-human-pluripotent-stem-cell-derived-endothelial-cells-in-2d-or-3d-culture
#14
Jue Zhang, Michael P Schwartz, Zhonggang Hou, Yongsheng Bai, Hamisha Ardalani, Scott Swanson, John Steill, Victor Ruotti, Angela Elwell, Bao Kim Nguyen, Jennifer Bolin, Ron Stewart, James A Thomson, William L Murphy
A defined protocol for efficiently deriving endothelial cells from human pluripotent stem cells was established and vascular morphogenesis was used as a model system to understand how synthetic hydrogels influence global biological function compared with common 2D and 3D culture platforms. RNA sequencing demonstrated that gene expression profiles were similar for endothelial cells and pericytes cocultured in polyethylene glycol (PEG) hydrogels or Matrigel, while monoculture comparisons identified distinct vascular signatures for each cell type...
March 10, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28343120/nanoemulgel-using-a-bicephalous-heterolipid-as-a-novel-approach-to-enhance-transdermal-permeation-of-tenofovir
#15
Sanjeev Rambharose, Rahul S Kalhapure, Thirumala Govender
Improvements in permeation enhancement strategies, such as nanoemulsions (NEs) and nanoemulgels (NEGs), have led to a renewed interest in transdermal drug delivery (TDD). This study aimed to investigate the potential of LLA1E, a novel dendritic permeation enhancer, as an oily phase in the development of a NEG for the TDD of tenofovir (TNF). TNF loaded NEs (TNEs) were prepared and analysed for mean globule diameter (MGD), polydispersity index (PDI), zeta potential (ZP) and morphology. NEGs of the TNEs (TNEGs) were prepared and evaluated for ex vivo transdermal permeation efficacy...
March 19, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28343005/development-of-a-uv-crosslinked-biodegradable-hydrogel-containing-adipose-derived-stem-cells-to-promote-vascularization-for-skin-wounds-and-tissue-engineering
#16
Gozde Eke, Naside Mangir, Nesrin Hasirci, Sheila MacNeil, Vasif Hasirci
The aim of this study was to design a dermal substitute containing adipose derived stem cells (ADSC) that can be used to improve the regeneration of skin on difficult wound beds by stimulating rapid neovascularization. This was achieved by first synthesizing methacrylated gelatin (GelMA) and methacrylated hyaluronic acid (HAMA) precursors which could be stored at -80 (o)C after lyophilisation. Polymer precursors were then dissolved in media (in 15:1 ratio), ADSCs added together with the photoinitiator and crosslinked with 40 s of UV...
March 18, 2017: Biomaterials
https://www.readbyqxmd.com/read/28342296/control-of-osmotic-pressure-to-improve-cell-viability-in-cell-laden-tissue-engineering-constructs
#17
A F Carvalho, L Gasperini, R S Ribeiro, A P Marques, R L Reis
Design of tissue engineering strategies deals with the need to balance both biomaterials characteristics and techniques specificities, often resulting in cell-compromising processing conditions. One important factor often disregarded is the osmotic pressure to which cells are exposed to. An in-house microfluidic system was used to prove that addition of an osmotic regulator significantly benefits the generation of viable cell-laden hydrogels under harsh processing conditions. Human adipose-derived stem cells (hASCs) were resuspended in 1...
March 25, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28340712/a-double-mediator-based-whole-cell-electrochemical-biosensor-for-acute-biotoxicity-assessment-of-wastewater
#18
Guanyue Gao, Deyu Fang, Yuan Yu, Liangzhuan Wu, Yu Wang, Jinfang Zhi
This work investigates the feasibility and sensitivity of a double-mediator based whole cell electrochemical biosensor to detect the acute biotoxicity of wastewater. The lipophilic mediator menadione was used to mediate the intracellular metabolic activities whereas hydrophilic potassium ferricyanide was employed as extracellular electron acceptor to transport the electron from the menadiol to anode. A chitosan hydrogel polymer film with boron-doped nanocrystalline diamond (BND) particles was electrodeposited onto a glassy carbon (GC) electrode to immobilize Saccharomyces cerevisiae cells and the mediators...
May 15, 2017: Talanta
https://www.readbyqxmd.com/read/28340537/study-on-a-3d-hydrogel-based-culture-model-for-characterizing-growth-of-fibroblasts-under-viral-infection-and-drug-treatment
#19
Xiaolu Zhu, Xianting Ding
Three-dimensional (3D) in vitro tissue models provide an approach for the systematic, repetitive, and quantitative study of drugs. In this study, we constructed an in vitro 3D acrylated hyaluronic acid (AHA) hydrogel model encapsulating fibroblasts, performed long-period 3D culture, and tested cellular topological changes and proliferation variation in the presence of herpes simplex virus-1 (HSV-1) as an infecting virus and acyclovir (ACV) as the treatment drug. The AHA hydrogels were formed by using Michael addition chemistry of bis-cysteine containing MMP-degradable cross-linker onto AHA prefunctionalized with cell adhesion peptides (RGD)...
March 1, 2017: SLAS Discov
https://www.readbyqxmd.com/read/28338415/hyaluronic-acid-hydrogel-functionalized-with-self-assembled-micelles-of-amphiphilic-pegylated-kartogenin-for-the-treatment-of-osteoarthritis
#20
Mi-Lan Kang, Se-Young Jeong, Gun-Il Im
Synthetic hyaluronic acid (HA) containing a covalently integrated drug is capable of releasing therapeutic molecules and is an attractive candidate for the intra-articular treatment of osteoarthritis (OA). Herein, self-assembled PEGylated kartogenin (PEG/KGN) micelles consisting of hydrophilic polyethylene glycol (PEG) and hydrophobic KGN, which has been shown to induce chondrogenesis in human mesenchymal stem cells, were prepared by covalent crosslinking. HA hydrogels containing PEG/KGN micelles (HA/PEG/KGN) were prepared by covalently bonding PEG chains to HA...
March 24, 2017: Tissue Engineering. Part A
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