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Joaquim Carreras, Yara Y Kikuti, Sílvia Beà, Masashi Miyaoka, Shinichiro Hiraiwa, Haruka Ikoma, Ryoko Nagao, Sakura Tomita, David Martin-Garcia, Itziar Salaverria, Ai Sato, Akifumi Ichiki, Giovanna Roncador, Juan F Garcia, Kiyoshi Ando, Elias Campo, Naoya Nakamura
AIMS: We aimed to define the clinicopathological characteristics of 29 primary sinonasal diffuse large B cell lymphoma (DLBCL(sn) ) in a series of 240 cases of DLBCL not otherwise specified [DLBCL(all ()(NOS)()) ], including DLBCL(sn) training set (n = 11) and validation set (n = 18), and DLBCL(non-sn) (n = 211). METHODS AND RESULTS: In the training set, 82% had a non-germinal center B-cell-like (Hans' Classifier) (non-GCB) phenotype and 18% were Epstein-Barr virus-encoded small RNAs (EBER)(+) ...
March 2017: Histopathology
T S Gan'shina, E V Kurza, I N Kurdyumov, D V Maslennikov, R S Mirzoyan
Experiments on nonlinear rats subjected to global transient cerebral ischemia revealed the ability of glutamic acid to improve cerebral circulation. Consequently, the excitatory amino acid can produce adverse (neurotoxic) and positive (anti-ischemic) effects in cerebral ischemia. The cerebrovascular effect of glutamic acid in cerebral ischemia is attenuated on the background action of the MNDA receptor blocker MK-801 (0.5 mg/kg intravenously) and eliminated by bicuculline. When glutamic acid is combined with the non-competitive MNDA receptor antagonist MK-801, neither one nor another drug shows its vasodilator effect...
2016: Eksperimental'naia i Klinicheskaia Farmakologiia
Michiel van den Brand, Jos Rijntjes, Konnie M Hebeda, Laura Menting, Carolyn V Bregitha, Wendy B C Stevens, Walter J F M van der Velden, Bastiaan B J Tops, J Han J M van Krieken, Patricia J T A Groenen
AIMS: To investigate the spectrum of mutations in 20 genes involved in B-cell receptor and/or Toll-like receptor signalling resulting in activation of nuclear factor-κB (NF-κB) in 20 nodal marginal zone lymphomas (NMZLs), 20 follicular lymphomas (FLs), and 11 cases of B-cell lymphoma, unclassifiable (BCL-u). METHODS AND RESULTS: Nodal marginal zone lymphomas were diagnosed according to strict criteria, including the expression of at least one putative marginal zone marker (MNDA and/or IRTA1)...
January 2017: Histopathology
V Somasundaram, S Soni, A Chopra, S Rai, M Mahapatra, R Kumar, H Pati
INTRODUCTION: The diagnosis of myelodysplastic syndrome (MDS) based on morphology is particularly difficult in low-grade MDS. Thus, the role of myeloid nuclear differentiation antigen (MNDA) and other flow cytometric (FCM) parameters in MDS was evaluated. METHODS: Bone marrow aspirates (BMA) collected from 52 patients with unexplained persistent cytopenias were divided into three groups: (i) proven MDS (n = 12) based on morphology and/or cytogenetics; (ii) suspected MDS (n = 6), noncontributory morphology, and cytogenetics; and (iii) non-MDS (n = 34)...
April 2016: International Journal of Laboratory Hematology
Ryan C Johnson, Jinah Kim, Yasodha Natkunam, Uma Sundram, Aharon G Freud, Bryan Gammon, Michael J Cascio
Myeloid neoplasms constitute one of the most common malignancies in adults. In most cases these proliferations initially manifest in the blood and marrow; however, extramedullary involvement may precede blood or marrow involvement in a subset of cases, making a definitive diagnosis challenging by morphologic and immunohistochemical assessment alone. Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare, aggressive entity that frequently presents in extramedullary sites and can show morphologic and immunophenotypic overlap with myeloid neoplasms...
April 2016: American Journal of Surgical Pathology
Vladan P Čokić, Pascal Mossuz, Jing Han, Nuria Socoro, Bojana B Beleslin-Čokić, Olivera Mitrović, Tijana Subotički, Miloš Diklić, Danijela Leković, Mirjana Gotić, Raj K Puri, Constance Tom Noguchi, Alan N Schechter
The gene and protein expression profiles in myeloproliferative neoplasms (MPNs) may reveal gene and protein markers of a potential clinical relevance in diagnosis, treatment and prediction of response to therapy. Using cDNA microarray analysis of 25,100 unique genes, we studied the gene expression profile of CD34+ cells and granulocytes obtained from peripheral blood of subjects with essential thrombocythemia (ET), polycythemia vera (PV) and primary myelofibrosis (PMF). The microarray analyses of the CD34+ cells and granulocytes were performed from 20 de novo MPN subjects: JAK2 positive ET, PV, PMF subjects, and JAK2 negative ET/PMF subjects...
2015: PloS One
Frauke Bellos, Wolfgang Kern
BACKGROUND: Confirming diagnosis of myelodysplastic syndromes (MDS) is often challenging. Standard diagnostic methods are cytomorphology (CM) and cytogenetics. Multiparameter flow cytometry (MFC) is upcoming in MDS diagnostic work up, comparability and investigator experiences are critical. Myeloid nuclear differentiation antigen (MNDA) in myelomonocytic cells might be expressed more weakly in patients with MDS. The analysis of MNDA may thus improve diagnostic capabilities of MFC in MDS...
July 17, 2015: Cytometry. Part B, Clinical Cytometry
(no author information available yet)
BACKGROUND: Gastrostomy feeding is commonly used to support patients with amyotrophic lateral sclerosis who develop severe dysphagia. Although recommended by both the American Academy of Neurology and the European Federation of Neurological Societies, currently little evidence indicates the optimum method and timing for gastrostomy insertion. We aimed to compare gastrostomy insertion approaches in terms of safety and clinical outcomes. METHODS: In this large, longitudinal, prospective cohort study (ProGas), we enrolled patients with a diagnosis of definite, probable, laboratory supported, or possible amyotrophic lateral sclerosis who had agreed with their treating clinicians to undergo gastrostomy at 24 motor neuron disease care centres or clinics in the UK...
July 2015: Lancet Neurology
Benjamin A Diner, Tuo Li, Todd M Greco, Marni S Crow, John A Fuesler, Jennifer Wang, Ileana M Cristea
The human PYHIN proteins, AIM2, IFI16, IFIX, and MNDA, are critical regulators of immune response, transcription, apoptosis, and cell cycle. However, their protein interactions and underlying mechanisms remain largely uncharacterized. Here, we provide the interaction network for all PYHIN proteins and define a function in sensing of viral DNA for the previously uncharacterized IFIX protein. By designing a cell-based inducible system and integrating microscopy, immunoaffinity capture, quantitative mass spectrometry, and bioinformatics, we identify over 300 PYHIN interactions reflective of diverse functions, including DNA damage response, transcription regulation, intracellular signaling, and antiviral response...
January 2015: Molecular Systems Biology
Frauke Bellos, Wolfgang Kern
Background: Confirming diagnosis of myelodysplastic syndromes (MDS) is often challenging. Standard diagnostic methods are cytomorphology (CM) and cytogenetics (CG). Multiparameter flow cytometry (MFC) is upcoming in MDS diagnostic work up, comparability and investigator experience are critical. Myeloid nuclear differentiation antigen (MNDA) in myelomonocytic cells might be expressed more weakly in patients with MDS. The analysis of MNDA may thus improve diagnostic capabilities of MFC in MDS. Methods: Staining methods and antibody combinations for MFC in MDS are outlined, giving details for interpretation of results in regard to dyspoiesis...
September 25, 2014: Cytometry. Part B, Clinical Cytometry
Shannon L Rigler, Denise M Kay, Robert J Sicko, Ruzong Fan, Aiyi Liu, Michele Caggana, Marilyn L Browne, Charlotte M Druschel, Paul A Romitti, Lawrence C Brody, James L Mills
PURPOSE: Heterotaxy is a clinically and genetically heterogeneous disorder. We investigated whether screening cases restricted to a classic phenotype would result in the discovery of novel, potentially causal copy-number variants. METHODS: We identified 77 cases of classic heterotaxy from all live births in New York State during 1998-2005. DNA extracted from each infant's newborn dried blood spot was genotyped with a microarray containing 2.5 million single-nucleotide polymorphisms...
May 2015: Genetics in Medicine: Official Journal of the American College of Medical Genetics
Dympna J Connolly, Andrew G Bowie
The innate immune response depends on the ability of immune cells to detect pathogens through germline-encoded pattern recognition receptors (PRRs). Recently discovered PRRs include some members of the Pyrin and HIN domain (PYHIN) family, which are encoded on an interferon-inducible gene cluster located on chromosome 1q23. There are five human PYHIN proteins; Absent in melanoma 2 (AIM2), IFN-γ inducible protein 16 (IFI16), Myeloid cell nuclear differentiation antigen (MNDA), Pyrin and HIN domain family member 1 (PYHIN1) and the recently identified Pyrin domain only protein 3 (POP3)...
December 1, 2014: Biochemical Pharmacology
E Popova
In order to evaluate the site of action of picrotoxin (antagonist of ionotropic GABA receptors) on the electroretinographic (ERG) b- and d-waves, in this study we compared its effects on the intensity-response function of the ERG waves in intact light adapted frog eyecup preparations with its effects in eyecups, where the activity of proximal neurons was blocked by 1 mMN-methyl-d-aspartate (MNDA). Picrotoxin markedly enhanced the b- and d-wave amplitude and slowed the time course of the responses at all stimulus intensities in the intact eyecups...
August 2014: Vision Research
Ryan A Metcalf, Ahmad Monabati, Monika Vyas, Giovanna Roncador, Gabriela Gualco, Carlos E Bacchi, Sheren F Younes, Yasodha Natkunam, Aharon G Freud
The diagnosis of marginal zone lymphomas (MZL) is challenged by the lack of specific markers that distinguish them from other low-grade non-Hodgkin B-cell lymphomas. Myeloid cell nuclear differentiation antigen (MNDA) is a nuclear protein that labels myelomonocytic cells as well as B lymphocytes that localize to the marginal zone areas of splenic white pulp. We evaluated MNDA expression in a large series of B-cell lymphomas to assess the sensitivity and specificity of this antigen for the characterization of MZL...
August 2014: Human Pathology
Rachele Cagliani, Diego Forni, Mara Biasin, Manuel Comabella, Franca R Guerini, Stefania Riva, Uberto Pozzoli, Cristina Agliardi, Domenico Caputo, Sunny Malhotra, Xavier Montalban, Nereo Bresolin, Mario Clerici, Manuela Sironi
AIM2-like receptors (ALRs) are a family of nucleic acid sensors essential for innate immune responses against viruses and bacteria. We performed an evolutionary analysis of ALR genes (MNDA, PYHIN1, IFI16, and AIM2) by analyzing inter- and intraspecies diversity. Maximum-likelihood analyses indicated that IFI16 and AIM2 evolved adaptively in primates, with branch-specific selection at the catarrhini lineage for IFI16. Application of a population genetics-phylogenetics approach also allowed identification of positive selection events in the human lineage...
April 2014: Genome Biology and Evolution
He Li, Zhi-Xin Wang, Jia-Wei Wu
The HIN domain of myeloid nuclear differentiation antigen (MNDA) was expressed and purified as a monomer using E. coli JM109 as host. The protein interacted with double-stranded DNA at a Kd of 3.15 μM and did not recognize the termini of double-stranded DNA. Isothermal titration calorimetry indicated that the interaction between the protein and double-stranded DNA is mainly mediated by electrostatic attractions and hydrogen bonding. We developed a model to analyze the potential DNA binding site of the MNDA HIN domain...
May 2014: Biotechnology Letters
Chengliang Sun, Chuanju Liu, Jun Dong, Dong Li, Wei Li
Despite improvements over the past two decades, the outcome for patients with advanced osteosarcoma remains poor. Targeted therapies have emerged as promising treatment options for various malignancies. However, effective targeted cancer therapies require the identification of key molecules in the pathogenesis of cancer. The aim of this study was to evaluate the value of the myeloid cell nuclear differentiation antigen (MNDA), a member of the interferon-inducible p200 (IFI-200) family, as a therapeutic target for osteosarcoma by analyzing the baseline expression of MNDA in human osteosarcoma cells and determining the effect of MNDA overexpression on the proliferation and apoptosis profiles and migration/invasion ability in osteosarcoma cells...
March 2014: Oncology Letters
Sumitha K Reddy, Anna Rosengren, Sylvia Klaubauf, Tejas Kulkarni, Eva Nordberg Karlsson, Ronald P de Vries, Henrik Stålbrand
Phylogenetic analysis of glycoside hydrolase family 2 including Aspergillus sequences and characterised β-mannosidases from other organisms, clusters putative Aspergillus β-mannosidases in two distinct clades (A and B). Aspergillus species have at least one paralog in each of the two clades. It appears that clade A members are extracellular and clade B members intracellular. Substrate specificity analysis of MndA of Aspergillus niger (clade A) and MndB of Aspergillus nidulans (clade B) show that MndB, in contrast to MndA, does not hydrolyse polymeric mannan and has probably evolved to hydrolyse small unbranched β-mannosides like mannobiose...
November 1, 2013: FEBS Letters
Yulan Jin, Ashok Sharma, Colleen Carey, Diane Hopkins, Xiaoxiao Wang, David G Robertson, Bruce Bode, Stephen W Anderson, John Chip Reed, R Dennis Steed, Leigh Steed, Jin-Xiong She
OBJECTIVE: Our previous gene expression microarray studies identified a number of genes differentially expressed in patients with type 1 diabetes (T1D) and islet autoantibody-positive subjects. This study was designed to validate these gene expression changes in T1D patients and to identify gene expression changes in diabetes complications. RESEARCH DESIGH AND METHODS: We performed high-throughput real-time RT-PCR to validate gene expression changes in peripheral blood mononuclear cells (PBMCs) from a large sample set of 928 T1D patients and 922 control subjects...
September 2013: Diabetes Care
Eric Milot, Nasser Fotouhi-Ardakani, János G Filep
Sepsis and septic shock are characterized by prolonged inflammation and delayed resolution, which are associated with suppression of neutrophil apoptosis. The role of the intrinsic apoptotic pathway and intracellular factors in regulation of neutrophil apoptosis remain incompletely understood. We previously reported that the nuclear factor MNDA (myeloid nuclear differentiation antigen) is fundamental to execution of the constitutive neutrophil death program. During neutrophil apoptosis MNDA is cleaved by caspases and relocated to the cytoplasm...
2012: Frontiers in Immunology
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