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https://www.readbyqxmd.com/read/28073499/physiological-and-transcriptional-responses-to-inorganic-nutrition-in-a-tropical-pacific-strain-of-alexandrium-minutum-implications-for-the-saxitoxin-genes-and-toxin-production
#1
Kieng Soon Hii, Po Teen Lim, Nyuk Fong Kon, Yoshinobu Takata, Gires Usup, Chui Pin Leaw
Saxitoxins (STXs) constitute a family of potent sodium channel blocking toxins, causative agents of paralytic shellfish poisoning (PSP), and are produced by several species of marine dinoflagellates and cyanobacteria. Two STX-core genes, sxtA and sxtG, have been well elucidated in Alexandrium but the expression of these genes under various nutritional modes in tropical species remains unclear. This study investigates the physiological responses of a tropical Pacific strain of Alexandrium minutum growing with nitrate or ammonium, and with various nitrogen to phosphorus (N:P) supply ratios...
June 2016: Harmful Algae
https://www.readbyqxmd.com/read/28070555/molecular-ligand-modulation-of-palladium-nanocatalysts-for-highly-efficient-and-robust-heterogeneous-oxidation-of-cyclohexenone-to-phenol
#2
Teng Xue, Zhaoyang Lin, Chin-Yi Chiu, Yongjia Li, Lingyan Ruan, Gongming Wang, Zipeng Zhao, Chain Lee, Xiangfeng Duan, Yu Huang
Metallic nanoparticles are emerging as an exciting class of heterogeneous catalysts with the potential advantages of exceptional activity, stability, recyclability, and easier separation than homogeneous catalysts. The traditional colloid nanoparticle syntheses usually involve strong surface binding ligands that could passivate the surface active sites and result in poor catalytic activity. The subsequent removal of surface ligands could reactivate the surface but often leads to metal ion leaching and/or severe Ostwald ripening with diminished catalytic activity or poor stability...
January 2017: Science Advances
https://www.readbyqxmd.com/read/28065270/functional-study-of-the-vitamin-k-cycle-enzymes-in-live-cells
#3
J-K Tie, D W Stafford
Vitamin K-dependent carboxylation, an essential posttranslational modification catalyzed by gamma-glutamyl carboxylase, is required for the biological functions of proteins that control blood coagulation, vascular calcification, bone metabolism, and other important physiological processes. Concomitant with carboxylation, reduced vitamin K (KH2) is oxidized to vitamin K epoxide (KO). KO must be recycled back to KH2 by the enzymes vitamin K epoxide reductase and vitamin K reductase in a pathway known as the vitamin K cycle...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28062838/enzymes-as-modular-catalysts-for-redox-half-reactions-in-h2-powered-chemical-synthesis-from-biology-to-technology
#4
REVIEW
Holly A Reeve, Philip A Ash, HyunSeo Park, Ailun Huang, Michalis Posidias, Chloe Tomlinson, Oliver Lenz, Kylie A Vincent
The present study considers the ways in which redox enzyme modules are coupled in living cells for linking reductive and oxidative half-reactions, and then reviews examples in which this concept can be exploited technologically in applications of coupled enzyme pairs. We discuss many examples in which enzymes are interfaced with electronically conductive particles to build up heterogeneous catalytic systems in an approach which could be termed synthetic biochemistry We focus on reactions involving the H(+)/H2 redox couple catalysed by NiFe hydrogenase moieties in conjunction with other biocatalysed reactions to assemble systems directed towards synthesis of specialised chemicals, chemical building blocks or bio-derived fuel molecules...
January 15, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28055175/multifunctional-mesoporous-ionic-gels-and-scaffolds-derived-from-polyhedral-oligomeric-silsesquioxanes
#5
Jin Hong Lee, Albert S Lee, Jong-Chan Lee, Soon Man Hong, Seung Sang Hwang, Chong Min Koo
A new methodology for fabrication of inorganic-organic hybrid ionogels and scaffolds is developed through facile crosslinking and solution extraction of a newly developed ionic polyhedral oligomeric silsesquioxane with inorganic core. Through design of various cationic tertiary amines as well as crosslinkable functional groups on each arm of the inorganic core, high performance ionogels are fabricated with excellent electrochemical stability and unique ion conduction behavior, giving superior lithium ion battery performance...
January 5, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28049432/identification-of-genes-affecting-alginate-biosynthesis-in-pseudomonas-fluorescens-by-screening-a-transposon-insertion-library
#6
Helga Ertesvåg, Håvard Sletta, Mona Senneset, Yi-Qian Sun, Geir Klinkenberg, Therese Aursand Konradsen, Trond E Ellingsen, Svein Valla
BACKGROUND: Polysaccharides often are necessary components of bacterial biofilms and capsules. Production of these biopolymers constitutes a drain on key components in the central carbon metabolism, but so far little is known concerning if and how the cells divide their resources between cell growth and production of exopolysaccharides. Alginate is an industrially important linear polysaccharide synthesized from fructose 6-phosphate by several bacterial species. The aim of this study was to identify genes that are necessary for obtaining a normal level of alginate production in alginate-producing Pseudomonas fluorescens...
January 3, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28047839/th-cd-206-08-an-anthropopathic-deformable-phantom-for-geometric-and-dose-accumulation-accuracy-validation-of-deformable-image-registration
#7
Y Liao, H Chen, J Chen, X Gu, X Zhen, L Zhou
PURPOSE: To design and construct a three-dimensional (3D) anthropopathic abdominal phantom for evaluating deformable image registration (DIR) accuracy on images and dose deformation in adaptive radiation therapy (ART). METHOD: Organ moulds, including liver, kidney, spleen, stomach, vertebra and two metastasis tumors, are 3D printed using the contours from an ovarian cancer patient. The organ moulds are molded with deformable gels that made of different mixtures of polyvinyl chloride (PVC) and the softener dioctyl terephthalate...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28046136/effects-of-dietary-urea-concentration-and-zilpaterol-hydrochloride-on-performance-and-carcass-characteristics-of-finishing-steers
#8
K L Samuelson, M E Hubbert, C A Löest
Cattle receiving zilpaterol hydrochloride () may recycle less N and require a greater supply of RDP. This study evaluated effects of ZH on performance and carcass characteristics of steers fed diets with increasing dietary RDP concentrations supplied as urea. Steers (429 animals; BW = 423 ± 4.5 kg) were sorted into 3 blocks according to BW and assigned to 1 of 6 treatments (6 pens per treatment) in a randomized complete block design. Treatments were a 2 × 3 factorial arrangement of either no ZH or ZH (75 mg ZH per steer daily) supplemented to finishing diets containing 0, 0...
December 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/28043123/reduction-of-c1-substrates-to-hydrocarbons-by-the-homometallic-precursor-and-synthetic-mimic-of-the-nitrogenase-cofactor
#9
Nathaniel S Sickerman, Kazuki Tanifuji, Chi Chung Lee, Yasuhiro Ohki, Kazuyuki Tatsumi, Markus W Ribbe, Yilin Hu
Solvent-extracted nitrogenase cofactors can reduce C1 substrates (CN(-), CO and CO2) to hydrocarbons in reactions driven by a strong reductant, SmI2 (E(0') = -1.55 V vs SCE). Here we show that a synthetic [Et4N]4[Fe6S9(SEt)2] cluster (designated the Fe6(RHH)-cluster), which mimics the homometallic [Fe8S9C] core of the nitrogenase cofactor (designated the L-cluster), is capable of conversion of C1 substrates into hydrocarbons in the same reactions. Comparison of the yields and product profiles between these homometallic clusters and their heterometallic counterparts points to possible roles of the heterometal, interstitial carbide and belt sulfur-bridged iron atoms in catalysis...
January 6, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28040727/a-conserved-retromer-sorting-motif-is-essential-for-mitochondrial-dlp1-recycling-by-vps35-in-parkinson-s-disease-model
#10
Wenzhang Wang, Xiaopin Ma, Leping Zhou, Jun Liu, Xiongwei Zhu
Impaired mitochondria dynamics and quality control are involved in mitochondrial dysfunction and pathogenesis of Parkinson's disease (PD). VPS35 mutations cause autosomal dominant PD and we recently demonstrated that fPD-associated VPS35 mutants can cause mitochondrial fragmentation through enhanced VPS35-DLP1 interaction. In this study, we focused on the specific sites on DLP1 responsible for the VPS35-DLP1 interaction. A highly conserved FLV motif was identified in the C-terminus of DLP1, mutation of which significantly reduced VPS35-DLP1 interaction...
December 30, 2016: Human Molecular Genetics
https://www.readbyqxmd.com/read/28039942/metallosalen-based-ionic-porous-polymers-as-bifunctional-catalysts-for-the-conversion-of-co2-into-valuable-chemicals
#11
Rongchang Luo, Yaju Chen, Qian He, Xiaowei Lin, Qihang Xu, Xiaohui He, Wuying Zhang, Xiantai Zhou, Hongbing Ji
A series of novel metallosalen-based ionic porous organic polymers (POPs) were synthesized for the first time using a simple unique strategy from the free-radical copolymerization reaction. Various techniques were used to characterize the physicochemical properties of these catalysts. It was found that the well-designed materials endowed high surface area, hierarchical porous structures and superior CO2/N2 adsorptive selectivity. Moreover, these POPs having both metal centers (Lewis acid) and ionic units (nuclephile) could be served as bifunctional catalysts in the catalytic conversion of CO2 into high value-added chemicals without any additional cocatalyst under mild and solvent-free conditions, e...
December 31, 2016: ChemSusChem
https://www.readbyqxmd.com/read/28039820/selective-electrochemical-degradation-of-4-chlorophenol-at-a-ti-ruo2-iro2-anode-in-chloride-rich-wastewater
#12
Jonas De Coster, Wouter Vanherck, Lise Appels, Raf Dewil
The electrochemical degradation of 4-chlorophenol (4-CP) in the presence of chlorides by the use of a Ti/RuO2-IrO2 anode was investigated under different operational parameters such as applied current density (J) and chloride concentration ([NaCl]). By performing a design of experiments, a model for the removal of 4-chlorophenol under these circumstances was obtained. To investigate matrix effects for this oxidation process, the influence of various biodegradable substrates (such as glucose, a complex synthetic wastewater and a pilot-scale UASB effluent) on the degradation profile of 4-chlorophenol was investigated...
December 28, 2016: Journal of Environmental Management
https://www.readbyqxmd.com/read/28035046/the-pleckstrin-homology-domain-of-dynamin-is-dispensable-for-membrane-constriction-and-fission
#13
Srishti Dar, Thomas J Pucadyil
Classical dynamins bind the plasma membrane-localized phosphatidylinositol-4,5-bisphosphate using the pleckstrin-homology domain (PHD) and engage in rapid membrane fission during synaptic vesicle recycling. This domain is conspicuously absent among extant bacterial and mitochondrial dynamins, however, where loop regions manage membrane recruitment. Inspired by the core design of bacterial and mitochondrial dynamins, we reengineered the classical dynamin by replacing its PHD with a polyhistidine or polylysine linker...
January 1, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28032761/iron-catalysis-for-modular-pyrimidine-synthesis-through-%C3%AE-ammoniation-cyclization-of-saturated-carbonyl-compounds-with-amidines
#14
Xue-Qiang Chu, Wen-Bin Cao, Xiao-Ping Xu, Shun-Jun Ji
An efficient method for the modular synthesis of various pyrimidine derivatives by means of the reactions of ketones, aldehydes, or esters with amidines in the presence of an in situ prepared recyclable iron(II)-complex was developed. This operationally simple reaction proceeded with broad functional group tolerance in a regioselective manner via a remarkable unactivated β-C-H bond functionalization. Control experiments were performed to gain deep understanding of the mechanism, and the reactions are likely to proceed through a designed TEMPO complexation/enamine addition/transient α-occupation/β-TEMPO elimination/cyclization sequence...
January 9, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28027045/role-of-occupational-therapy-in-preventing-work-related-musculoskeletal-disorders-with-recycling-workers-a-pilot-study
#15
Thomas Fisher
Little is known about injury prevention in recycling workers; therefore, in this study, I aimed to identify physical and psychosocial risk factors for employees involved in single-stream recycling and to explore strategies for prevention and wellness. Fifteen participants who were either recycling workers or supervisors located throughout seven locations on an urban university campus participated. A mixed-methods research design was used to obtain data through a variety of standardized work environment and health questionnaires and semistructured interviews...
January 2017: American Journal of Occupational Therapy: Official Publication of the American Occupational Therapy Association
https://www.readbyqxmd.com/read/28013071/from-fat-to-fat-cd36-sr-b2-understanding-the-regulation-of-cellular-fatty-acid-uptake
#16
Jan F C Glatz, Joost J F P Luiken
The molecular mechanisms underlying the cellular uptake of long-chain fatty acids and the regulation of this process have been elucidated in appreciable detail in the last decades. Two main players in this field, each discovered in the early 1990s, are (i) a membrane-associated protein first identified in adipose ('fat') tissue and referred to as putative fatty acid translocase (FAT)/CD36 (now officially designated as SR-B2) which facilitates the transport of fatty acids across the plasma membrane, and (ii) the family of transcription factors designated peroxisome proliferator-activated receptors (PPARα, PPARγ, and PPARβ/δ) for which fatty acids and fatty acid metabolites are the preferred ligand...
December 21, 2016: Biochimie
https://www.readbyqxmd.com/read/28011085/self-excising-cre-mutant-lox-marker-recycling-system-for-multiple-gene-integrations-and-consecutive-gene-deletions-in-aspergillus-oryzae
#17
Silai Zhang, Akihiko Ban, Naoki Ebara, Osamu Mizutani, Mizuki Tanaka, Takahiro Shintani, Katsuya Gomi
In this study, we developed a self-excising Cre/loxP-mediated marker recycling system with mutated lox sequences to introduce a number of biosynthetic genes into Aspergillus oryzae. To construct the self-excising marker cassette, both the selectable marker, the Aspergillus nidulans adeA gene, and the Cre recombinase gene (cre), conditionally expressed by the xylanase-encoding gene promoter, were designed to be located between the mutant lox sequences, lox66 and lox71. However, construction of the plasmid failed, possibly owing to a slight expression of cre downstream of the fungal gene promoter in Escherichia coli...
December 20, 2016: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/27987444/environmental-friendly-technology-for-aluminum-electrolytic-capacitors-recycling-from-waste-printed-circuit-boards
#18
Jianbo Wang, Zhenming Xu
up to now, the recycling of e-waste should be developed towards more depth and refinement to promote industrial production of e-waste resource recovery. in the present study, the recycling of aluminum electrolytic capacitors (AECs) from waste printed circuit boards (WPCBs) is focused on. First of all, AECs are disassembled from WPCBs by a self-designed machine; meanwhile, the disassembled AECs are subjected to an integrated process, involving heating treatment, crushing, sieving, and magnetic separating, to recover aluminum and iron; finally, the off-gas and residue generated during the aforementioned processes are analyzed to evaluate environmental risks...
December 8, 2016: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/27979107/green-and-efficient-extraction-of-rutin-from-tartary-buckwheat-hull-by-using-natural-deep-eutectic-solvents
#19
Yao Huang, Fang Feng, Jie Jiang, Ying Qiao, Tao Wu, Josef Voglmeir, Zhi-Gang Chen
In this study, an efficient extraction technique using a combination of ultrasound and natural deep eutectic solvents (NADESs) was developed. Some basic physical properties, including viscosity, polarity, and solubility, of thirteen NADESs prepared from natural components were investigated systematically. Results show that the solubility of rutin increased in choline chloride- and glycerol-based NADESs by 660-1577times compared to water. NADESs with high rutin extractability can be designed by combining NADESs components...
April 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27979071/a-recyclable-protein-resource-derived-from-cauliflower-by-products-potential-biological-activities-of-protein-hydrolysates
#20
Yang Xu, Yuting Li, Tao Bao, Xiaodong Zheng, Wei Chen, Jianxu Wang
Cauliflower by-products (CBP) are rich in leaf protein. Every year tons of CBP will lead to environmental pollution. Therefore, this study was conducted to extract leaf protein from CBP and investigate its biological activities. Our results showed that the optimal extraction parameters were: a liquid to solid ratio of 4mL/g, a pH of 11, an ultrasonic extraction lasting 15min, and at an applied power of 175W. Under these optimized conditions, 12.066g of soluble leaf protein (SLP) was obtained from 1000g of CBP and its extraction yield was 53...
April 15, 2017: Food Chemistry
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