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https://www.readbyqxmd.com/read/27771064/a-pasteurella-multocida-strain-affecting-nulliparous-heifers-and-calves-in-different-ways
#1
Conny Turni, Denise Dayao, Gorka Aduriz, Nekane Cortabarria, Carolina Tejero, Jose C Ibabe, Reema Singh, Pat Blackall
Pasteurella multocida isolates from dairy cattle on a farm in Spain were associated with pneumonia of calves (six isolates) and mastitis of heifers (five isolates). The objective was to determine if the P. multocida isolates retrieved from both disease scenarios were the same strain or whether more than one strain was present. The isolates were identified by a species-specific polymerase chain (PCR) assay, serotyped by the Heddleston scheme and then typed by a number of molecular genotyping assays including multi-locus sequence typing (MLST)...
November 15, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/27727405/resistance-to-linezolid-in-staphylococcus-spp-clinical-isolates-associated-with-ribosomal-binding-site-modifications-novel-mutation-in-domain-v-of-23s-rrna
#2
Rosario Musumeci, Enrico Calaresu, Jolanda Gerosa, Davide Oggioni, Simone Bramati, Patrizia Morelli, Ida Mura, Andrea Piana, Bianca Maria Are, Clementina Elvezia Cocuzza
Linezolid is the main representative of the oxazolidinones, introduced in 2000 in clinical practice to treat severe Gram-positive infections. This compound inhibits protein synthesis by binding to the peptidyl transferase centre of the 50S bacterial ribosomal subunit. The aim of this study was to characterize 12 clinical strains of linezolid-resistant Staphylococcus spp. isolated in Northern Italy. All isolates of Staphylococcus spp. studied showed a multi-antibiotic resistance phenotype. In particular, all isolates showed the presence of the mecA gene associated with SSCmec types IVa, V or I...
September 13, 2016: New Microbiologica
https://www.readbyqxmd.com/read/27707768/marrow-adipose-tissue-spectrum-in-obesity-and-type-2-diabetes-mellitus
#3
Iana M de Araujo, Carlos E G Salmon, Andressa K Nahas, Marcello H Nogueira-Barbosa, Jorge Elias Junior, Francisco Ja de Paula
OBJECTIVE: To assess the association of bone mass and marrow adipose tissue (MAT) with other fat depots, insulin resistance, bone remodeling markers, adipokines and glucose control in type 2 diabetes and obesity. DESIGN AND METHODS: Cross-sectional study; comprised 24 controls (C), 26 obese (O) and 28 type 2 diabetes. Dual-energy X-ray absorptiometry was used to determine bone mineral density (BMD). Blood samples were collected for biochemical measurements. 1H Magnetic resonance spectroscopy was used to assess MAT in the L3 vertebra and abdominal magnetic resonance imaging was used to assess intrahepatic lipids, visceral (VAT) and subcutaneous adipose tissue...
October 5, 2016: European Journal of Endocrinology
https://www.readbyqxmd.com/read/27649071/classification-of-high-intensity-zones-of-the-lumbar-spine-and-their-association-with-other-spinal-mri-phenotypes-the-wakayama-spine-study
#4
Masatoshi Teraguchi, Dino Samartzis, Hiroshi Hashizume, Hiroshi Yamada, Shigeyuki Muraki, Hiroyuki Oka, Jason Pui Yin Cheung, Ryohei Kagotani, Hiroki Iwahashi, Sakae Tanaka, Hiroshi Kawaguchi, Kozo Nakamura, Toru Akune, Kenneth Man-Chee Cheung, Noriko Yoshimura, Munehito Yoshida
INTRODUCTION: High intensity zones (HIZ) of the lumbar spine are a phenotype of the intervertebral disc noted on MRI whose clinical relevance has been debated. Traditionally, T2-weighted (T2W) magnetic resonance imaging (MRI) has been utilized to identify HIZ of lumbar discs. However, controversy exists with regards to HIZ morphology, topography, and association with other MRI spinal phenotypes. Moreover, classification of HIZ has not been thoroughly defined in the past and the use of additional imaging parameters (e...
2016: PloS One
https://www.readbyqxmd.com/read/27559167/partial-breakdown-of-input-specificity-of-stdp-at-individual-synapses-promotes-new-learning
#5
Maxim Volgushev, Jen-Yung Chen, Vladimir Ilin, Roman Goz, Marina Chistiakova, Maxim Bazhenov
UNLABELLED: Hebbian-type learning rules, which underlie learning and refinement of neuronal connectivity, postulate input specificity of synaptic changes. However, theoretical analyses have long appreciated that additional mechanisms, not restricted to activated synapses, are needed to counteract positive feedback imposed by Hebbian-type rules on synaptic weight changes and to achieve stable operation of learning systems. The biological basis of such mechanisms has remained elusive. Here we show that, in layer 2/3 pyramidal neurons from slices of visual cortex of rats, synaptic changes induced at individual synapses by spike timing-dependent plasticity do not strictly follow the input specificity rule...
August 24, 2016: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/27543534/a-cadaver-study-comparing-spread-of-dye-and-nerve-involvement-after-three-different-quadratus-lumborum-blocks
#6
L Carline, G A McLeod, C Lamb
BACKGROUND: Posterior variants of abdominal wall block include the quadratus lumborum type I, quadratus lumborum type II and quadratus lumborum transmuscular blocks. Our objectives were to compare the spread of injectate and nerve involvement, after conducting blocks using ultrasound guidance in soft embalmed cadavers. METHODS: After randomization, an experienced anaesthetist conducted three quadratus lumborum 1, three quadratus lumborum 2 and four transmuscular blocks on the left or right sides of five cadavers...
September 2016: British Journal of Anaesthesia
https://www.readbyqxmd.com/read/27497888/the-fatty-degeneration-of-lumbar-paraspinal-muscles-on-computed-tomography-scan-according-to-age-and-disc-level
#7
Shin Heon Lee, Seung Won Park, Young Baeg Kim, Taek Kyun Nam, Young Seok Lee
BACKGROUND CONTEXT: Spinal degeneration can occur not only in the bone and disc but also in muscles. Fatty infiltration (FI) and decreased volume have been described as characteristic changes that occur in muscle degeneration. Many studies about the lumbar paraspinal muscles have been conducted on patients with spine problems. However, the natural changes of age-dependent degeneration in the paraspinal muscles have not been studied properly. PURPOSE: The purpose of this study is to investigate age- and level-dependent changes of the lumbar paraspinal muscles in the population without lumbar spinal symptoms...
August 4, 2016: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/27436292/detailed-morphology-of-all-life-stages-of-the-agave-red-worm-comadia-redtenbacheri-hammerschmidt-lepidoptera-cossidae
#8
R Castro-Torres, C Llanderal-Cázares
The agave red worm, Comadia redtenbacheri (Hammerschmidt), is an important source of food and income in Mexico. Despite its importance, several aspects of its biology, morphology, and behavior remain poorly studied. In this work, we describe and illustrate the morphology of all the life stages that may aid in understanding certain aspects of its biology. To obtain all life stages, last instar larvae were collected from agave plants and allowed to pupate; after the adults emerged, they were allowed to mate and oviposit...
July 19, 2016: Neotropical Entomology
https://www.readbyqxmd.com/read/27433426/a-biomechanical-comparison-of-shape-design-and-positioning-of-transforaminal-lumbar-interbody-fusion-cages
#9
Garet C Comer, Anthony Behn, Shashank Ravi, Ivan Cheng
STUDY DESIGN: Cadaveric biomechanical analysis. OBJECTIVE: The aim of this study was to compare three interbody cage shapes and their position within the interbody space with regards to construct stability for transforaminal lumbar interbody fusion. METHODS: Twenty L2-L3 and L4-L5 lumbar motion segments from fresh cadavers were potted in polymethyl methacrylate and subjected to testing with a materials testing machine before and after unilateral facetectomy, diskectomy, and interbody cage insertion...
August 2016: Global Spine Journal
https://www.readbyqxmd.com/read/27406956/computer-aided-detection-and-classification-of-acute-lymphoblastic-leukemia-cell-subtypes-based-on-microscopic-image-analysis
#10
Morteza MoradiAmin, Ahmad Memari, Nasser Samadzadehaghdam, Saeed Kermani, Ardeshir Talebi
Acute lymphoblastic leukemia (ALL) is a cancer that starts from the early version of white blood cells called lymphocytes in the bone marrow. It can spread to different parts of the body rapidly and if not treated, would probably be deadly within a couple of months. Leukemia cells are categorized into three types of L1, L2, and L3. The cancer is detected through screening of blood and bone marrow smears by pathologists. But manual examination of blood samples is a time-consuming and boring procedure as well as limited by human error risks...
July 13, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27364167/fusion-angle-affects-intervertebral-adjacent-spinal-segment-joint-forces-model-based-analysis-of-patient-specific-alignment
#11
Marco Senteler, Bernhard Weisse, Dominique A Rothenfluh, Mazda T Farshad, Jess G Snedeker
This study addresses the hypothesis that adjacent segment intervertebral joint loads are sensitive to the degree of lordosis that is surgically imposed during vertebral fusion. Adjacent segment degeneration is often observed after lumbar fusion, but a causative mechanism is not yet clearly evident. Altered kinematics of the adjacent segments and potentially nonphysiological mechanical joint loads have been implicated in this process. However, little is known of how altered alignment and kinematics influence loading of the adjacent intervertebral joints under consideration of active muscle forces...
July 1, 2016: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/27355713/suture-choice-in-lumbar-dural-closure-contributes-to-variation-in-leak-pressures-experimental-model
#12
George M Ghobrial, Christopher M Maulucci, Matthew J Viereck, Sara Beygi, Ameet Chitale, Srinivas Prasad, Jack Jallo, Joshua Heller, Ashwini D Sharan, James S Harrop
STUDY DESIGN: Open-label Laboratory Investigational Study; Non-animal Surgical Simulation OBJECTIVES:: The authors perform a comparison of dural closure strength in a durotomy simulator across two different suture materials. SUMMARY OF BACKGROUND DATA: Incidental durotomy leading to persistent cerebrospinal fluid (CSF) leak adds considerable morbidity to spinal procedures, often complicating routine elective lumbar spinal procedures. Using an experimental durotomy simulation, the authors compare the strength of closure using Gore-tex™ with other suture types and sizes, using various closure techniques...
June 28, 2016: Clinical Spine Surgery
https://www.readbyqxmd.com/read/27338229/bone-formation-and-degradation-behavior-of-nanocrystalline-hydroxyapatite-with-or-without-collagen-type-1-in-osteoporotic-bone-defects-an-experimental-study-in-osteoporotic-goats
#13
Volker Alt, Wing Hoi Cheung, Simon K H Chow, Ulrich Thormann, Edmond N M Cheung, Katrin S Lips, Reinhard Schnettler, Kwok-Sui Leung
The intention of the current work is to assess new bone formation and degradation behavior of nanocrystalline hydroxyapatite with (HA/col-1) or without collagen-type I (HA) in osteoporotic metaphyseal bone defects in goats. After ovariectomy and special low-calcium diet for three months, 3 drill hole defects in the vertebrae of L3, L4, L5, 4 drill hole defects in the right and left iliac crest and 1 drill hole defect at the distal femur were created in three Chinese mountain goats with a total of 24 defects...
June 2016: Injury
https://www.readbyqxmd.com/read/27333058/outcomes-of-surgery-in-patients-with-lumbar-spinal-canal-stenosis-comparison-of-three-types-of-stenosis-on-mri
#14
Parisa Azimi, Shirzad Azhari, Edward C Benzel, Hamid Khayat Kashany, Hossein Nayeb Aghaei, Hassan Reza Mohammadi, Meysam Ebrahimi
The aim of the study was to compare outcome of surgery in patients with lumbar canal stenosis (LCS) based on magnetic resonance imaging (MRI) morphology. This was a prospective study of 96 consecutive patients who underwent surgery at 143 levels of LCS (from L3-L4 to L5-S1). Using patterns on T2 axial MRI, the type of stenosis was determined for each patient. The Swiss Spinal Stenosis Score (SSS) was used to evaluate patients' functionality and outcomes. The definition of treatment success was based on the criteria developed by Stucki et al...
2016: PloS One
https://www.readbyqxmd.com/read/27326958/minimizing-pedicle-screw-pullout-risks-a-detailed-biomechanical-analysis-of-screw-design-and-placement
#15
Rohan-Jean Bianco, Pierre-Jean Arnoux, Eric Wagnac, Jean-Marc Mac-Thiong, Carl-Éric Aubin
STUDY DESIGN: Detailed biomechanical analysis of the anchorage performance provided by different pedicle screw design and placement strategies under pullout loading. OBJECTIVE: To biomechanically characterize the specific effects of surgeon-specific pedicle screw design parameters on anchorage performance using a finite element model (FEM). SUMMARY OF BACKGROUND DATA: Pedicle screw fixation is commonly used in the treatment of spinal pathologies...
June 20, 2016: Clinical Spine Surgery
https://www.readbyqxmd.com/read/27324605/the-natural-history-of-modic-changes-in-a-community-based-cohort
#16
Andrew J Teichtahl, Monica A Finnin, Yuanyuan Wang, Anita E Wluka, Donna M Urquhart, Richard O'Sullivan, Graeme Jones, Flavia M Cicuttini
BACKGROUND: Vertebral endplate (Modic) lesions are gaining interest, with varied phenotypes recognised to have distinct clinical and histological correlates. Nevertheless, the natural history of these lesions is unclear. This study examined the natural history of Modic changes and their potential relationship to the intervertebral disc. METHODS: Seventy-two community-based adults not selected for low back pain had lumbar spine magnetic resonance imaging (MRI) performed at baseline (2012) and approximately 2 years later to assess Modic lesions...
June 17, 2016: Joint, Bone, Spine: Revue du Rhumatisme
https://www.readbyqxmd.com/read/27285821/investigating-clinical-failure-of-bone-grafting-through-a-window-at-the-femoral-head-neck-junction-surgery-for-the-treatment-of-osteonecrosis-of-the-femoral-head
#17
Wei Zuo, Wei Sun, Dingyan Zhao, Fuqiang Gao, Yangming Su, Zirong Li
AIMS: This study aimed to analyze the clinical factors related to the failure of bone grafting through a window at the femoral head-neck junction. METHODS: In total, 119 patients (158 hips) underwent bone grafting for treatment of avascular necrosis of the femoral head. The patients were classified by their ARCO staging and CJFH classification. All patients were clinically and radiographically followed up every three months during the first year and every six months in the following year...
2016: PloS One
https://www.readbyqxmd.com/read/27283772/missense-mutations-in-the-tp53-dna-binding-domain-predict-outcomes-in-patients-with-advanced-oral-cavity-squamous-cell-carcinoma
#18
Nina Lapke, Yen-Jung Lu, Chun-Ta Liao, Li-Yu Lee, Chien-Yu Lin, Hung-Ming Wang, Shu-Hang Ng, Shu-Jen Chen, Tzu-Chen Yen
TP53 mutations have been linked to reduced survival in patients with oral cavity squamous cell carcinoma (OSCC). However, the impact of different types of TP53 mutations remains unclear. Here, we demonstrate that the carriage of missense mutations in the TP53 DNA binding domain (DBD missense mutations) is associated with decreased disease-specific survival (DSS) compared with wild-type TP53 (P=0.002) in a cohort of 345 OSCC patients. In contrast, DSS of patients bearing all of the remaining TP53 mutations did not differ from that observed in wild-type TP53 patients (P=0...
June 8, 2016: Oncotarget
https://www.readbyqxmd.com/read/27272472/development-of-on-chip-fully-automated-immunoassay-system-%C3%AE-taswako-i30-to-measure-the-changes-in-glycosylation-profiles-of-alpha-fetoprotein-in-patients-with-hepatocellular-carcinoma
#19
Tatsuo Kurosawa, Mitsuo Watanabe
Glycosylation profiles significantly change during oncogenesis. Aberrant glycosylation can be used as a cancer biomarker in clinical settings. Different glycoforms can be separately detected using lectin affinity electrophoresis and lectin array-based methods. However, most methodologies and procedures need experienced technique to perform the assays and expertise to interpret the results. To apply glycomarkers for clinical practice, a robust assay system with an easy-to-use workflow is required. Wako's μTASWako i30, a fully automated immunoanalyzer, was developed for in vitro diagnostics based on microfluidic technology...
June 8, 2016: Proteomics
https://www.readbyqxmd.com/read/27267215/phenylobacterium-aquaticum-sp-nov-isolated-from-the-reservoir-of-a-water-purifier
#20
Jung Hun Jo, Gyu-Min Choi, Soon-Youl Lee, Wan-Taek Im
A Gram-reaction-negative, strictly aerobic, non-motile, non-spore-forming, rod-shaped bacterial strain designated W2-3-4T was isolated from the reservoir of a water purifier. This bacterium was characterized to determine its taxonomic position by using a polyphasic approach. Strain W2-3-4T grew well at 25-30 °C on nutrient and R2A agars. On the basis of 16S rRNA gene sequence similarity, strain W2-3-4T was shown to belong to the family Caulobacteraceaeand to be related to Phenylobacterium conjunctumFWC21T (98...
September 2016: International Journal of Systematic and Evolutionary Microbiology
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