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https://www.readbyqxmd.com/read/29046938/is-there-a-negative-association-between-the-content-of-condensed-tannins-total-phenols-and-total-tannins-of-tropical-plant-extracts-and-in-vitro-anthelmintic-activity-against-haemonchus-contortus-eggs
#1
G S Castañeda-Ramírez, J F J Torres-Acosta, C A Sandoval-Castro, P G González-Pech, V P Parra-Tabla, C Mathieu
In vitro studies using plant extracts suggest a relationship between their polyphenol contents and their anthelmintic (AH) activity against Haemonchus contortus. High polyphenol content appears to increase the efficacy of plant extracts against H. contortus as assessed by the larval exsheathment inhibition assay (LEIA) while appearing to reduce the AH efficacy measured using the egg hatch assay (EHA). In addition, some plants lack AH activity. Therefore, the present study investigated the relationship between the contents of condensed tannins (CT), total phenols (TP), and total tannins (TT) in methanol:water extracts (70:30) obtained from ten tropical plant species consumed by small ruminants as well as their AH activity against H...
October 18, 2017: Parasitology Research
https://www.readbyqxmd.com/read/29038898/molecular-and-immunological-characterisation-of-tropomyosin-from-anisakis-pegreffii
#2
Abdouslam Asnoussi, Ibukun E Aibinu, Robin B Gasser, Andreas L Lopata, Peter M Smooker
Tropomyosin (TM) is a major allergen in shellfish, known to cross-react with mite, cockroach and/or some roundworm (nematode) TM. In this study, we aimed to express and purify TM from the parasitic nematode Anisakis pegreffii and also to characterise its cross-reactivity with TM from shellfish. A. pegreffii was isolated from the flathead tiger fish (Neoplatycephalus richardsoni) and characterised using single-strand conformation polymorphism (SSCP)-based sequencing of the first and second internal transcribed spacers (ITS-1 and ITS-2) of nuclear ribosomal DNA...
October 16, 2017: Parasitology Research
https://www.readbyqxmd.com/read/29038812/sonogashira-cu-and-amine-free-and-suzuki-coupling-in-air-catalyzed-via-nanoparticles-formed-in-situ-from-pd-ii-complexes-of-chalcogenated-schiff-bases-of-1-naphthaldehyde-and-their-reduced-forms
#3
Renu Bhaskar, Alpesh K Sharma, Manoj K Yadav, Ajai K Singh
The reaction of 1-naphthaldehyde with 2-(phenylthio/seleno)ethylamine afforded air- and moisture-insensitive Schiff bases: C10H7-1-CH[double bond, length as m-dash]N-CH2CH2EPh (L1: E = S; L2: E = Se). Then, on treatment with NaOAc and Li2PdCl4, palladacycles, [Pd(L1-H/L2-H)Cl] (1/2) were formed at room temperature, in which L1/L2 are ligated as an unsymmetric (C(-), N, E) pincer. The reduction of >C[double bond, length as m-dash]N bonds of L1 and L2 with sodium borohydride gave C10H7-1-CH2NH-CH2CH2EPh (L3: E = S; L4: E = Se)...
October 17, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/29035857/humoral-response-of-mice-infected-with-toxocara-canis-following-different-infection-schemes
#4
Jan Novák, Lucie Panská, Tomáš Macháček, Libuše Kolářová, Petr Horák
The study was focused on the dynamics of humoral response to Toxocara canis excretory-secretory antigens (TES antigens) in mice experimentally infected by T. canis L3 larvae in different ways. In particular, we compared the effect of infection with two doses of 1000 larvae vs. repeated infections with a low number of larvae (daily infection with 10 larvae and weekly infection with 100 larvae in the course of 22 weeks). In ELISA, all infections, including both schemes with lower larval doses, elicited significant antibody response...
December 20, 2017: Acta Parasitologica
https://www.readbyqxmd.com/read/29032017/editorial-next-generation-sequencing-technology-a-new-tool-for-killer-cell-immunoglobulin-like-receptor-allele-typing-in-hematopoietic-stem-cell-transplantation
#5
B Maniangou, C Retière, K Gagne
Killer cell Immunoglobulin-like Receptor (KIR) genes are a family of genes located together within the leukocyte receptor cluster on human chromosome 19q13.4. To date, 17 KIR genes have been identified including nine inhibitory genes (2DL1/L2/L3/L4/L5A/L5B, 3DL1/L2/L3), six activating genes (2DS1/S2/S3/S4/S5, 3DS1) and two pseudogenes (2DP1, 3DP1) classified into group A (KIR A) and group B (KIR B) haplotypes. The number and the nature of KIR genes vary between the individuals. In addition, these KIR genes are known to be polymorphic at allelic level (907 alleles described in July 2017)...
October 11, 2017: Transfusion Clinique et Biologique: Journal de la Société Française de Transfusion Sanguine
https://www.readbyqxmd.com/read/29022168/vertebral-heights-and-ratios-are-not-only-race-specific-but-also-gender-and-region-specific-establishment-of-reference-values-for-mainland-chinese
#6
Lei Ning, Li-Jiang Song, Shun-Wu Fan, Xing Zhao, Yi-Lei Chen, Zhao-Zhi Li, Zi-Ang Hu
This study established gender-specific reference values in mainland Chinese (MC) and is important for quantitative morphometry for diagnosis and epidemiological study of osteoporotic vertebral compressive fracture. Comparisons of reference values among different racial populations are then performed to demonstrate the MC-specific characteristic. PURPOSE: Osteoporotic vertebral compressive fracture (OVCF) is a common complication of osteoporosis in the elder population. Clinical diagnosis and epidemiological study of OVCF often employ quantitative morphometry, which relies heavily on the comparison of patients' vertebral parameters to existing reference values derived from the normal population...
October 11, 2017: Archives of Osteoporosis
https://www.readbyqxmd.com/read/29020990/a-comparison-of-two-different-anthelmintic-treatment-regimens-against-natural-gastrointestinal-nematode-infections-on-two-lithuanian-sheep-farms
#7
Tomas Kupcinskas, Inga Stadaliene, Algimantas Paulauskas, Pavelas Trusevicius, Saulius Petkevicius, Johan Höglund, Mindaugas Sarkunas
BACKGROUND: According to targeted treatment (TT), the whole flock is dewormed based on knowledge of the risk, or parameters that quantify the mean level of infection, whereas according to targeted selective treatment (TST), only individual animals within the grazing group are treated, based on parasitological, production and/or morbidity parameters. The aim of this study was to compare two different treatment protocols on sheep farms in Lithuania. The study was conducted from 15 April to 31 October 2014 on three sheep farms...
October 11, 2017: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/28989841/should-we-label-all-synovial-cysts-as-unstable
#8
Arvind G Kulkarni, Shumayou Dutta, Abhilash Dhruv, Anupreet Bassi
STUDY DESIGN: Retrospective study. OBJECTIVE: To analyze the various anatomical parameters that influence segmental stability in patients suffering from lumbar intra spinal cysts (LISCs) and to determine the outcome of microscopic unilateral laminotomy and cystectomy. METHODS: All patients that were surgically managed for a LISC between 2007 and 2013 with more than 3 years of follow-up were reviewed. Those without associated instability were evaluated for segmental mobility, segmental angulation, facet inclination, stage of disc degeneration, and level of involvement on MRI and dynamic radiographs...
October 2017: Global Spine Journal
https://www.readbyqxmd.com/read/28988262/playing-the-clarinet-influence-of-body-posture-on-muscle-activity-and-sound-quality
#9
V Ae Baadjou, Mdf van Eijsden-Besseling, Jamcf Verbunt, R A de Bie, Rpj Geers, Rjem Smeets, Ham Seelen
Musculoskeletal complaints are highly prevalent in clarinetists and are related to high arm load while playing. It is hypothesized that postural exercise therapy may be used to adapt muscle activity patterns while playing and thus contribute to better sound quality. The goal of the present study was to investigate the relationship between body posture, muscle activity, and sound quality in clarinetists while playing the instrument in two different postures, their habitual sitting posture (control, CO) vs an experimental sitting posture (EXP) based on Mensendieck postural exercise therapy, method Samama...
September 2017: Medical Problems of Performing Artists
https://www.readbyqxmd.com/read/28983962/etiology-of-developmental-spinal-stenosis-a-genome-wide-association-study
#10
Jason Pui Yin Cheung, Patrick Yp Kao, Pak Sham, Kathryn Se Cheah, Danny Chan, Kenneth Mc Cheung, Dino Samartzis
Our study aimed to identify possible single nucleotide polymorphisms (SNPs) via a genome-wide association study (GWAS) approach and a candidate gene platform that were associated with lumbar developmental spinal stenosis (DSS). Southern Chinese population-based study volunteers were assessed (age range: 18-55 years). DSS was defined as the anteroposterior bony spinal canal diameter on T1-weighted axial MRI of L1 to S1. Genotyping was performed using the Illumina HumanOmniZhongHua-8 BeadChip. Using the canal diameter as the quantitative trait, genomic statistical analyses was performed...
October 6, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28975709/ubiquitin-conjugating-enzyme-e2-l3-is-downregulated-by-the-chikungunya-virus-nsp2-protease
#11
Suwipa Ramphan, Sarawut Khongwichit, Chonticha Saisawang, Duangnapa Kovanich, Albert J Ketterman, Sukathida Ubol, Prasert Auewarakul, Sittiruk Roytrakul, Duncan R Smith, Atichat Kuadkitkan
PURPOSE: Chikungunya virus (CHIKV) is a mosquito transmitted alphavirus that causes chikungunya fever in humans. The CHIKV nonstructural protein 2 (nsP2) is a multifunctional protein that additionally modulates the host cell to dampen the innate immune response and inhibit other cellular processes. EXPERIMENTAL DESIGN: To further investigate the interactions of nsP2 with host cells, the protease domain of CHIKV nsP2 (nsP2-pro) was transfected into Hela cells, and differential protein expression was detected by 2-dimension (2D) polyacrylamide gel electrophoresis...
October 4, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28973027/plant-expressed-pyocins-for-control-of-pseudomonas-aeruginosa
#12
Šarūnas Paškevičius, Urtė Starkevič, Audrius Misiūnas, Astra Vitkauskienė, Yuri Gleba, Aušra Ražanskienė
The emergence, persistence and spread of antibiotic-resistant human pathogenic bacteria heralds a growing global health crisis. Drug-resistant strains of gram-negative bacteria, such as Pseudomonas aeruginosa, are especially dangerous and the medical and economic burden they impose underscore the critical need for finding new antimicrobials. Recent studies have demonstrated that plant-expressed bacteriocins of the colicins family can be efficient antibacterials against all major enteropathogenic strains of E...
2017: PloS One
https://www.readbyqxmd.com/read/28970932/-miii2mii3-n-trigonal-bipyramidal-cages-based-on-diamagnetic-and-paramagnetic-metalloligands
#13
S Sanz, H M O'Connor, V Martí-Centelles, P Comar, M B Pitak, S J Coles, G Lorusso, E Palacios, M Evangelisti, A Baldansuren, N F Chilton, H Weihe, E J L McInnes, P J Lusby, S Piligkos, E K Brechin
A family of five [MIII2MII3] (n+) trigonal bipyramidal cages (M(III) = Fe, Cr and Al; M(II) = Co, Zn and Pd; n = 0 for 1-3 and n = 6 for 4-5) of formulae [Fe2Co3L6Cl6] (1), [Fe2Zn3L6Br6] (2), [Cr2Zn3L6Br6] (3), [Cr2Pd3L6(dppp)3](OTf)6 (4) and [Al2Pd3L6(dppp)3](OTf)6 (5) (where HL is 1-(4-pyridyl)butane-1,3-dione and dppp is 1,3-bis(diphenylphosphino)propane) are reported. Neutral cages 1-3 were synthesised using the tritopic [M(III)L3] metalloligand in combination with the salts Co(II)Cl2 and Zn(II)Br2, which both act as tetrahedral linkers...
August 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28969837/in-vitro-anthelmintic-and-cytotoxicity-activities-the-digitaria-insularis-poaceae
#14
Francianne Oliveira Santos, Hélimar Gonçalves de Lima, Nathália Silva de Souza Santos, Taiane Menezes Serra, Rosângela Soares Uzeda, Isabella Mary Alves Reis, Mariana Borges Botura, Alexsandro Branco, Maria José Moreira Batatinha
This study aimed to evaluate the in vitro activity of D. insularis extracts and fractions against gastrointestinal nematodes of goats and its cytotoxicity on Vero cells. The egg hatch (EHT) and larval motility (LMT) tests were conducted to investigate the anthelmintic effects of the crude hydroethanolic (CH), ethyl acetate (EA), butanolic (BT) and residual hydroethanolic (RH) extracts. The elution of the active extract (EA) on column chromatography (SiO2) using organic solvents furnished six fractions (FR1 to FR6), which were also tested...
October 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28969785/evaluation-of-serology-to-measure-exposure-of-piglets-to-ascaris-suum-during-the-nursery-phase
#15
Elise Vandekerckhove, Johnny Vlaminck, Peter Geldhof
The aim of this study was to evaluate whether serology can be used to measure exposure of piglets to Ascaris suum during the nursery phase. Experimental infection studies were performed in which 7 groups of 10 piglets of 4 weeks of age were orally infected with either 10, 20, 40, 60, 80, 100 and 500 A. suum eggs/day during 7 consecutive weeks, mimicking a nursery phase in an A. suum contaminated environment. Serum was collected on a weekly basis to monitor seroconversion on 2 ELISA tests based on the antibody recognition of either a haemoglobin protein purified from the pseudocoelomic fluid of adult A...
November 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28969719/high-dose-enoxaparin-in-the-treatment-of-abdominal-angiostrongyliasis-in-swiss-mice
#16
A S S Sandri, R Rodriguez, M M Costa, S M Porto, D Schwingel, M I B Vieira
Abdominal angiostrongyliasis (AA) is caused by Angiostrongylus costaricensis, which inhabits mesenteric arteries. There is no drug treatment for AA, and since intestinal infarction due to thrombi is one of the main complications of the disease, the use of anticoagulants may be a treatment option. Thus, we aimed to assess the effect of high doses of enoxaparin on the prevention of ischaemic intestinal lesions and on the survival of mice infected with A. costaricensis. Twenty-four mice were infected with L3 of A...
October 3, 2017: Journal of Helminthology
https://www.readbyqxmd.com/read/28962911/from-the-international-space-station-to-the-clinic-how-prolonged-unloading-may-disrupt-lumbar-spine-stability
#17
Jeannie F Bailey, Stephanie L Miller, Kristine Khieu, Conor W O'Neill, Robert M Healey, Dezba G Coughlin, Jojo V Sayson, Douglas G Chang, Alan R Hargens, Jeffrey C Lotz
Background context Prolonged microgravity exposure is associated with localized low back pain and an elevated risk of post-flight disc herniation. Though the mechanisms by which microgravity impairs the spine are unclear, they should be foundational for developing in-flight countermeasures for maintaining astronaut spine health. Because human spine anatomy has adapted to upright posture on Earth, observations of how spaceflight affects the spine should also provide new and potentially important information on spine biomechanics that benefit the general population...
September 26, 2017: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/28961701/zaaps-program-results-for-2015-an-activity-and-spectrum-analysis-of-linezolid-using-clinical-isolates-from-medical-centres-in-32-countries
#18
Michael A Pfaller, Rodrigo E Mendes, Jennifer M Streit, Patricia A Hogan, Robert K Flamm
Objectives: To report the linezolid in vitro activity obtained during the 2015 ZAAPS Program. Methods: In total, 7587 organisms causing documented infections were consecutively collected in 65 centres in 32 ex-USA countries. Broth microdilution susceptibility testing was performed. Isolates displaying linezolid MIC results of ≥ 4 mg/L were molecularly characterized. Results: Linezolid inhibited >99.9% of Staphylococcus aureus at ≤ 2 mg/L, with MIC 50 results of 1 mg/L, regardless of methicillin resistance...
July 25, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28953417/in-vitro-in-vivo-and-interaction-studies-of-nematophagous-fungus-arthrobotrys-thaumasia-monacrosporium-thaumasium-with-the-larvae-of-trichostrongylides-of-sheep
#19
Bo-Bo Wang, Kui-Zheng Cai, Qiang Xu, Jun-Lin Liu, Yu-Jia Xue, Kang-Ying Wang, Rui Ren, Wen-Xiu Fang, Fan Wang, Xin Cao
It is necessary to screen more potential candidates from the local isolates of nematophagous fungi and to investigate interaction between the fungal strains and the nematodes for the biological control of parasitic nematodes in livestocks. In the present study, The present study was divided into two phases. In the first phase, it was to bewe assessed that the in vitro predatory activity and the viability of isolates of Arthrobotrys thaumasia (Monacrosporium thaumasium) after passing through the gastrointestinal tract of sheep...
September 27, 2017: Journal of Parasitology
https://www.readbyqxmd.com/read/28946526/health-related-quality-of-life-hrqol-following-transforaminal-percutaneous-endoscopic-discectomy-tped-for-lumbar-disc-herniation-a-prospective-cohort-study-early-results
#20
S Kapetanakis, E Giovannopoulou, G Charitoudis, K Kazakos
BACKGROUND: Lumbar discectomy is among the most frequently performed procedures in spinal surgery. Transforaminal percutaneous endoscopic discectomy (TPED) is a minimally invasive technique that gains ground among surgeons in the recent years. TPED has been studied in terms of effectiveness, however little is known about its overall impact on health-related quality of life (HRQoL) of the patients. OBJECTIVE: To investigate the progress of HRQoL following TPED. PATIENTS AND METHODS: Seventy-six (76) patients were enrolled in the study...
June 10, 2017: Journal of Back and Musculoskeletal Rehabilitation
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