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Lipid transfer protein

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https://www.readbyqxmd.com/read/28644423/probing-the-interaction-of-dielectric-nanoparticles-with-supported-lipid-membrane-coatings-on-nanoplasmonic-arrays
#1
Abdul Rahim Ferhan, Gamaliel Junren Ma, Joshua A Jackman, Tun Naw Sut, Jae Hyeon Park, Nam-Joon Cho
The integration of supported lipid membranes with surface-based nanoplasmonic arrays provides a powerful sensing approach to investigate biointerfacial phenomena at membrane interfaces. While a growing number of lipid vesicles, protein, and nucleic acid systems have been explored with nanoplasmonic sensors, there has been only very limited investigation of the interactions between solution-phase nanomaterials and supported lipid membranes. Herein, we established a surface-based localized surface plasmon resonance (LSPR) sensing platform for probing the interaction of dielectric nanoparticles with supported lipid bilayer (SLB)-coated, plasmonic nanodisk arrays...
June 23, 2017: Sensors
https://www.readbyqxmd.com/read/28639287/exploration-of-conformational-changes-in-lactose-permease-upon-sugar-binding-and-proton-transfer-through-coarse-grained-simulations
#2
Yead Jewel, Prashanta Dutta, Jin Liu
Escherichia coli lactose permease (LacY) actively transports lactose and other galactosides across cell membranes through lactose/H(+) symport process. Lactose/H(+) symport is a highly complex process that involves sugar translocation, H(+) transfer, as well as large-scale protein conformational changes. The complete picture of lactose/H(+) symport is largely unclear due to the complexity and multiscale nature of the process. In this work, we develop the force field for sugar molecules compatible with PACE, a hybrid and coarse-grained force field that couples the united-atom protein models with the coarse-grained MARTINI water/lipid...
June 22, 2017: Proteins
https://www.readbyqxmd.com/read/28639207/the-cell-biology-of-the-thyroid-disrupting-mechanism-of-dichlorodiphenyltrichloroethane-ddt
#3
REVIEW
M Rossi, A R Taddei, I Fasciani, R Maggio, F Giorgi
OBJECTIVE: Dichlorodiphenyltrichloroethane (DDT) is an organochlorine known for its pesticide properties and for its negative effects on human health. It was banned in most countries for its toxicity to the endocrine system, but due to its persistence at clinically relevant concentrations in both soil and animal tissues, DDT is still linked to several health and social problems. METHODS: We have previously shown that DDT exposure is causally related to the extracellular release of vesicular organelles such as microvesicles and/or exosomes by using immunocytochemistry with gold-tagged antibodies and various fluorescent membrane markers...
June 21, 2017: Journal of Endocrinological Investigation
https://www.readbyqxmd.com/read/28633642/prediction-and-analysis-of-three-gene-families-related-to-leaf-rust-puccinia-triticina-resistance-in-wheat-triticum-aestivum-l
#4
Fred Y Peng, Rong-Cai Yang
BACKGROUND: The resistance to leaf rust (Lr) caused by Puccinia triticina in wheat (Triticum aestivum L.) has been well studied over the past decades with over 70 Lr genes being mapped on different chromosomes and numerous QTLs (quantitative trait loci) being detected or mapped using DNA markers. Such resistance is often divided into race-specific and race-nonspecific resistance. The race-nonspecific resistance can be further divided into resistance to most or all races of the same pathogen and resistance to multiple pathogens...
June 20, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28631440/recognition-cascade-and-metabolite-transfer-in-a-marine-bacteria-phytoplankton-model-system
#5
Bryndan P Durham, Stephen P Dearth, Shalabh Sharma, Shady A Amin, Christa B Smith, Shawn R Campagna, E Virginia Armbrust, Mary Ann Moran
The trophic linkage between marine bacteria and phytoplankton in the surface ocean is a key step in the global carbon cycle, with almost half of marine primary production transformed by heterotrophic bacterioplankton within hours to weeks of fixation. Early studies conceptualized this link as the passive addition and removal of organic compounds from a shared seawater reservoir. Here we analyzed transcript and intracellular metabolite patterns in a two-member model system and found that the presence of a heterotrophic bacterium induced a potential recognition cascade in a marine phytoplankton species that parallels better-understood vascular plant response systems...
June 19, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28623937/the-association-of-lipid-metabolism-relative-gene-polymorphisms-and-ischemic-stroke-in-han-and-uighur-population-of-xinjiang
#6
Yun-Hua Yue, Ling-Yun Liu, Liang Hu, You-Mei Li, Jie-Ping Mao, Xiao-Ying Yang, Na-Mu Dila
BACKGROUND: The present study is aimed to evaluate difference of lipid metabolism related gene single nucleotide polymorphisms (SNPs) with ischemic stroke (IS) in Han and Uighur population of Xinjiang, China. METHODS: Four hundred eight patients with ischemic stroke and 347 unrelated healthy individuals of age and sex matched were genotyped for Apolipoprotein A5 (ApoA5), lipoprotein lipase (LPL), Cholesteryl ester transfer protein (CETP) and low-density lipoprotein receptor (LDL-R) genes...
June 17, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28619636/isolation-and-characterization-of-a-non-specific-lipid-transfer-protein-from-chelidonium-majus-l-latex
#7
Robert Nawrot, Damian Jozefiak, Anna Sip, Dorota Kuzma, Oskar Musidlak, Anna Gozdzicka-Jozefiak, Jakub Barylski
Plant non-specific lipid transfer proteins (nsLTPs) are small basic proteins, which mostly play a role in intracellular lipid transport and antimicrobial defense. Recently it was shown using shotgun proteomic approach that the whole plant extract of Chelidonium majus L. (Papaveraceae) contains relatively abundant nsLTPs. Therefore the aim of the work was to isolate and characterize nsLTP from C. majus latex. RESULTS: obtained using PCR approach with degenerate primers showed nsLTP protein from C...
June 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28618157/liver-microsomal-lipid-enhances-the-activity-and-redox-coupling-of-co-localised-cytochrome-p450-reductase-cytochrome-p450-3a4-in-nanodiscs
#8
Kang-Cheng Liu, John M X Hughes, Sam Hay, Nigel S Scrutton
The haem-containing monooxygenase cytochrome P450 3A4 (CYP3A4) and its redox partner NADPH-dependent cytochrome P450 oxidoreductase (CPR) are among the most important enzymes in human liver for metabolising drugs and xenobiotic compounds. They are membrane-bound in the endoplasmic reticulum (ER). How ER co-localisation and the complex ER phospholipid composition influence enzyme activity are not well understood. CPR and CYP3A4 were incorporated into phospholipid bilayer nanodiscs, both singly, and together in a 1:1 ratio, to investigate the significance of membrane insertion and the influence of varying membrane composition on steady-state reaction kinetics...
June 15, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28612368/crystal-structure-of-non-specific-lipid-transfer-protein-from-solanum-melongena
#9
Abha Jain, Dinakar M Salunke
Lipids are considered to protect protein allergens from proteolysis and are generally seen to exist in a bound form. One of the well-known plant protein families with bound lipids is non-specific lipid transfer proteins (nsLTPs). Structure-function relationships in the case of the members of non-specific lipid transfer protein family are not clearly understood. As part of exploring the seed proteome, we have analyzed the proteome of a member of Solanaceae family, Solanum melongena (eggplant) and a non-specific lipid transfer protein from S...
June 13, 2017: Proteins
https://www.readbyqxmd.com/read/28611186/success-failure-and-transparency-in-biomarker-based-drug-development-a-case-study-of-cholesteryl-ester-transfer-protein-inhibitors
#10
Spencer Phillips Hey, Jessica M Franklin, Jerry Avorn, Aaron S Kesselheim
BACKGROUND: Although biomarkers are used as surrogate measures for drug targeting and approval and are generally based on plausible biological hypotheses, some are found to not correlate well with clinical outcomes. Over-reliance on inadequately validated biomarkers in drug development can lead to harm to trial subjects and patients and to research waste. To shed greater light on the process and ethics of biomarker-based drug development, we conducted a systematic portfolio analysis of cholesterol ester transfer protein inhibitors, a drug class designed to improve lipid profiles and prevent cardiovascular events...
June 2017: Circulation. Cardiovascular Quality and Outcomes
https://www.readbyqxmd.com/read/28607057/family-transmitted-stress-in-a-wild-bird
#11
José C Noguera, Sin-Yeon Kim, Alberto Velando
Recent data suggest that, in animals living in social groups, stress-induced changes in behavior have the potential to act as a source of information, so that stressed individuals could themselves act as stressful stimuli for other individuals with whom they interact repeatedly. Such form of cross-over of stress may be beneficial if it enhances adaptive responses to ecological stressors in the shared environment. However, whether stress can be transferred among individuals during early life in natural populations remains unknown...
June 12, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28605392/transfer-of-mammary-gland-forming-ability-between-mammary-basal-epithelial-cells-and-mammary-luminal-cells-via-extracellular-vesicles-exosomes
#12
Meng-Chieh Lin, Shih-Yin Chen, Pei-Lin He, Wen-Ting Luo, Hua-Jung Li
Cells can communicate via exosomes, ~100-nm extracellular vesicles (EVs) that contain proteins, lipids, and nucleic acids. Non-adherent/mesenchymal mammary epithelial cell (NAMEC)-derived extracellular vesicles can be isolated from NAMEC medium via differential ultracentrifugation. Based on their density, EVs can be purified via ultracentrifugation at 110,000 x g. The EV preparation from ultracentrifugation can be further separated using a continuous density gradient to prevent contamination with soluble proteins...
June 3, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28603593/markers-of-inflammation-and-cardiovascular-disease-in-recently-diagnosed-celiac-disease-patients
#13
Walter F Tetzlaff, Tomás Meroño, Martin Menafra, Maximiliano Martin, Eliana Botta, Maria D Matoso, Patricia Sorroche, Juan A De Paula, Laura E Boero, Fernando Brites
AIM: To evaluate novel risk factors and biomarkers of cardiovascular disease in celiac disease (CD) patients compared with healthy controls. METHODS: Twenty adult patients with recent diagnosis of CD and 20 sex, age and body mass index-matched healthy controls were recruited during a period of 12 mo. Indicators of carbohydrate metabolism, hematological parameters and high sensitive C reactive protein were determined. Moreover, lipoprotein metabolism was also explored through evaluation of the lipid profile and the activity of cholesteryl ester transfer protein and lipoprotein associated phospholipase A2, which is also considered a specific marker of vascular inflammation...
May 26, 2017: World Journal of Cardiology
https://www.readbyqxmd.com/read/28603533/transcriptome-analysis-reveals-differential-gene-expression-and-a-possible-role-of-gibberellins-in-a-shade-tolerant-mutant-of-perennial-ryegrass
#14
Wei Li, Lorenzo Katin-Grazzini, Xianbin Gu, Xiaojing Wang, Rania El-Tanbouly, Huseyin Yer, Chandra Thammina, John Inguagiato, Karl Guillard, Richard J McAvoy, Jill Wegrzyn, Tingting Gu, Yi Li
The molecular basis behind shade tolerance in plants is not fully understood. Previously, we have shown that a connection may exist between shade tolerance and dwarfism, however, the mechanism connecting these phenotypes is not well understood. In order to clarify this connection, we analyzed the transcriptome of a previously identified shade-tolerant mutant of perennial ryegrass (Lolium perenne L.) called shadow-1. shadow-1 mutant plants are dwarf, and are significantly tolerant to shade in a number of environments compared to wild-type controls...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28603518/microbial-extracellular-polymeric-substances-epss-in-ocean-systems
#15
REVIEW
Alan W Decho, Tony Gutierrez
Microbial cells (i.e., bacteria, archaea, microeukaryotes) in oceans secrete a diverse array of large molecules, collectively called extracellular polymeric substances (EPSs) or simply exopolymers. These secretions facilitate attachment to surfaces that lead to the formation of structured 'biofilm' communities. In open-water environments, they also lead to formation of organic colloids, and larger aggregations of cells, called 'marine snow.' Secretion of EPS is now recognized as a fundamental microbial adaptation, occurring under many environmental conditions, and one that influences many ocean processes...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28601811/microsomal-triglyceride-transfer-protein-mtp-in-the-ectoparasitic-crustacean-salmon-louse-l-salmonis
#16
Muhammad Tanveer Khan, Sussie Dalvin, Frank Nilsen, Rune Male
The salmon louse Lepeophtheirus salmonis is a challenging ectoparasite for the salmon farming industry and wild salmonids. Salmon lice have a very high reproductive output, that require deposition of large amounts of lipids into growing oocytes as a major energy source, most probably mediated by lipoproteins. Microsomal triglyceride transfer protein (MTP) is essential for the assembly of lipoproteins. Salmon lice have three LsMTP transcript variants encoding two different protein isoforms predicted to contain three β sheets (N, C and A) and a central helical domain, similar to MTP from other species...
June 10, 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/28598235/lift-and-cut-anti-host-autophagy-mechanism-of-legionella-pneumophila
#17
Supansa Pantoom, Aimin Yang, Yao-Wen Wu
RavZ, an effector protein of pathogenic Legionella pneumophila, inhibits host macroautophagy/autophagy by deconjugation of lipidated LC3 proteins from phosphatidylethanolamine (PE) on the autophagosome membrane. The mechanism for how RavZ specifically recognizes and deconjugates the lipidated LC3s is not clear. To understand the structure-function relationship of LC3-deconjugation by RavZ, we prepared semisynthetic LC3 proteins modified with different fragments of PE or 1-hexadecanol (C16). We find that RavZ activity is strictly dependent on the conjugated PE structure and RavZ extracts LC3-PE from the membrane before deconjugation...
June 9, 2017: Autophagy
https://www.readbyqxmd.com/read/28595554/sulphur-containing-amino-acids-protective-role-against-free-radicals-and-heavy-metals
#18
Mirjana B Čolović, Vesna M Vasić, Dragan M Djuric, Danijela Z Krstić
Sulphur is an abundant element in biological systems, which plays an important role in processes essential for life as a constituent of proteins, vitamins and other crucial biomolecules. The major source of sulphur for humans is plants being able to use inorganic sulphur in the purpose of sulphur-containing amino acids synthesis. Sulphur-containing amino acids include methionine, cysteine, homocysteine, and taurine. Among them, methionine and cysteine are classified as proteinogenic, canonic amino acids incorporated in proteins structure...
June 8, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28592883/the-lateral-distance-between-a-proton-pump-and-atp-synthase-determines-the-atp-synthesis-rate
#19
Johannes Sjöholm, Jan Bergstrand, Tobias Nilsson, Radek Šachl, Christoph von Ballmoos, Jerker Widengren, Peter Brzezinski
We have investigated the effect of lipid composition on interactions between cytochrome bo 3 and ATP-synthase, and the ATP-synthesis activity driven by proton pumping. The two proteins were labeled by fluorescent probes and co-reconstituted in large (d ≅ 100 nm) or giant (d ≅ 10 µm) unilamellar lipid vesicles. Interactions were investigated using fluorescence correlation/cross-correlation spectroscopy and the activity was determined by measuring ATP production, driven by electron-proton transfer, as a function of time...
June 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28592485/energetics-of-side-chain-partitioning-of-%C3%AE-signal-residues-in-unassisted-folding-of-a-transmembrane-%C3%AE-barrel-protein
#20
Bharat Ramasubramanian Iyer, Punit Zadafiya, Pallavi Vijay Vetal, Radhakrishnan Mahalakshmi
The free energy of water-to-interface amino acid partitioning is a major contributing factor in membrane protein folding and stability. The interface residues at the C-terminus of transmembrane β-barrels form the β-signal motif required for assisted β-barrel assembly in vivo, but are believed to be less important for β-barrel assembly in vitro Here, we experimentally measured the thermodynamic contribution of all 20 amino acids at the β-signal motif to the unassisted folding of the model β-barrel protein PagP...
June 7, 2017: Journal of Biological Chemistry
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