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Lipid transfer protein

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https://www.readbyqxmd.com/read/28743912/structural-basis-for-dolichylphosphate-mannose-biosynthesis
#1
Rosaria Gandini, Tom Reichenbach, Tien-Chye Tan, Christina Divne
Protein glycosylation is a critical protein modification. In biogenic membranes of eukaryotes and archaea, these reactions require activated mannose in the form of the lipid conjugate dolichylphosphate mannose (Dol-P-Man). The membrane protein dolichylphosphate mannose synthase (DPMS) catalyzes the reaction whereby mannose is transferred from GDP-mannose to the dolichol carrier Dol-P, to yield Dol-P-Man. Failure to produce or utilize Dol-P-Man compromises organism viability, and in humans, several mutations in the human dpm1 gene lead to congenital disorders of glycosylation (CDG)...
July 25, 2017: Nature Communications
https://www.readbyqxmd.com/read/28739288/recent-advances-on-extracellular-vesicles-in-therapeutic-delivery-challenges-solutions-and-opportunities
#2
REVIEW
Mei Lu, Haonan Xing, Zhen Yang, Yanping Sun, Tianzhi Yang, Xiaoyun Zhao, Cuifang Cai, Dongkai Wang, Pingtian Ding
Extracellular vesicles (EVs) are intrinsic mediators of intercellular communication in our body, allowing functional transfer of biomolecules (lipids, proteins, and nucleic acid) between diverse locations. Such an instrumental role evokes a surge of interest within the drug delivery community in tailoring EVs for therapeutic delivery. These vesicles represent a novel generation of drug delivery systems, providing high delivery efficiency, intrinsic targeting properties, and low immunogenicity. In the recent years, considerable research efforts have been directed toward developing safe and efficient EV-based delivery vehicles...
July 21, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28733595/how-cholesteryl-ester-transfer-protein-can-also-be-a-potential-triglyceride-transporter
#3
Venkat R Chirasani, Sanjib Senapati
CETP transfers cholesteryl esters (CEs) and triglycerides (TGs) between different lipoproteins and came in limelight as a drug-target against CVD. In the search for detailed mechanism of lipid transfer through CETP, enormous effort is devoted employing crystallographic, cryo-EM, and Molecular Dynamics (MD) studies. However, these studies primarily focused on CE-bound CETP structure and CE transfer mechanism. With the reported correlation that CETP looses significant CE transfer activity upon inhibiting TG transfer, it is of tremendous importance to understand the structure and dynamics of TG-bound CETP...
July 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28733164/the-role-of-oxysterol-binding-protein-and-its-related-proteins-in-cancer
#4
REVIEW
Ximing Du, Nigel Turner, Hongyuan Yang
Oxysterol-binding protein (OSBP) and its related proteins (ORPs) constitute a large, evolutionarily conserved family of lipid-binding proteins that are associated with a wide range of cellular activities. The core function of OSBP/ORPs appears to be moving lipids between cellular membranes in a non-vesicular manner. Recent studies have unveiled a novel, counter-transport mechanism of cellular lipid transfer mediated by OSBP/ORPs at the membrane contact sites that involves phosphatidylinositol 4-phosphate. Importantly, the OSBP/ORPs family has also been implicated in cell signalling pathways and cancer development...
July 18, 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/28731414/identification-of-a-lipid-transfer-protein-as-a-new-allergen-from-morus-alba-pollen
#5
D El-Qutob, B Bartolome, J Cuesta, A Mir, A Alberola, C Pastor-Vargas
No abstract text is available yet for this article.
2017: Journal of Investigational Allergology & Clinical Immunology
https://www.readbyqxmd.com/read/28729959/shot-gun-proteome-and-transcriptome-mapping-of-the-jujube-floral-organ-and-identification-of-a-pollen-specific-s-locus-f-box-gene
#6
Ruihong Chen, Guoliang Chen, Jian Huang
The flower is a plant reproductive organ that forms part of the fruit produced as the flowering season ends. While the number and identity of proteins expressed in a jujube (Ziziphus jujuba Mill.) flower is currently unknown, integrative proteomic and transcriptomic analyses provide a systematic strategy of characterizing the floral biology of plants. We conducted a shotgun proteomic analysis on jujube flowers by using a filter-aided sample preparation tryptic digestion, followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS)...
2017: PeerJ
https://www.readbyqxmd.com/read/28729676/proteomic-profiling-of-the-weed-feverfew-a-neglected-pollen-allergen-source
#7
Isabel Pablos, Stephanie Eichhorn, Peter Briza, Claudia Asam, Ulrike Gartner, Martin Wolf, Christof Ebner, Barbara Bohle, Naveen Arora, Stefan Vieths, Fatima Ferreira, Gabriele Gadermaier
Feverfew (Parthenium hysterophorus), an invasive weed from the Asteraceae family, has been reported as allergen source. Despite its relevance, knowledge of allergens is restricted to a partial sequence of a hydroxyproline-rich glycoprotein. We aimed to obtain the entire sequence for recombinant production and characterize feverfew pollen using proteomics and immunological assays. Par h 1, a defensin-proline fusion allergen was obtained by cDNA cloning and recombinantly produced in E. coli. Using two complementary proteomic strategies, a total of 258 proteins were identified in feverfew pollen among those 47 proteins belonging to allergenic families...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28727920/ballistic-penetration-of-highly-charged-nanoaerosol-particles-through-a-lipid-monolayer
#8
Victor N Morozov, Yuri M Shlyapnikov, Igor L Kanev, Elena Andreevna Shlyapnikova
To be used as a drug, inhaled nanoaerosol particles (NAPs) must first penetrate the lipid layer on top of the lung fluid before they will be able to reach the lung epithelium. We investigated how the penetration of NAPs through a model lipid monolayer (LM) depends upon their charging level and size. It was shown that deposition of NAPs 20-200 nm in diameter and charged to the Rayleigh limit gradually increased the surface tension of a dipalmitoylphosphatidylcholine monolayer (DPPC), indicating a loss of lipid molecules from the monolayer...
July 20, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28718511/genome-wide-identification-of-genes-involved-in-the-potato-response-to-drought-indicates-functional-evolutionary-conservation-with-arabidopsis-plants
#9
M Pieczynski, A Wyrzykowska, K Milanowska, D Boguszewska-Mankowska, B Zagdanska, W Karlowski, A Jarmolowski, Z Szweykowska-Kulinska
Potato is one of the four most important food crop plants worldwide and is strongly affected by drought. The following two pairs of potato cultivars, which are related in ancestry but show different drought tolerances, were chosen for comparative gene expression studies: Gwiazda/Oberon and Tajfun/Owacja. Comparative RNA-seq analyses of gene expression differences in the transcriptomes obtained from drought-tolerant versus drought-sensitive plants during water shortage conditions were performed. The twenty-three top-ranking genes were selected, 22 of which are described here as novel potato drought-responsive genes...
July 17, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28718450/dynamics-and-energetics-of-the-mammalian-phosphatidylinositol-transfer-protein-phospholipid-exchange-cycle
#10
Aby Grabon, Adam Orlowski, Ashutosh Tripathi, Joni Vuorio, Matti Javanainen, Tomasz Róg, Max Lönnfors, Mark I McDermott, Garland Siebert, Pentti Somerharju, Ilpo Vattulainen, Vytas A Bankaitis
Phosphatidylinositol-transfer proteins (PITPs) regulate phosphoinositide signaling in eukaryotic cells. The defining feature of PITPs is their ability to exchange phosphatidylinositol (PtdIns) molecules between membranes, and this property is central to PITP-mediated regulation of lipid signaling. Yet, the details of the PITP-mediated lipid exchange cycle remain entirely obscure. Here, all-atom molecular dynamics (MD) simulations of the mammalian StART-like PtdIns/ phosphatidylcholine (PtdCho) transfer protein PITPα, both on membrane bilayers and in solvated systems, informed downstream biochemical analyses that tested key aspects of the hypotheses generated by the MD simulations...
July 17, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28716048/transcriptome-analysis-uncovers-arabidopsis-f-box-stress-induced-1-as-a-regulator-of-jasmonic-acid-and-abscisic-acid-stress-gene-expression
#11
Lauren E Gonzalez, Kristen Keller, Karen X Chan, Megan M Gessel, Bryan C Thines
BACKGROUND: The ubiquitin 26S proteasome system (UPS) selectively degrades cellular proteins, which results in physiological changes to eukaryotic cells. F-box proteins are substrate adaptors within the UPS and are responsible for the diversity of potential protein targets. Plant genomes are enriched in F-box genes, but the vast majority of these have unknown roles. This work investigated the Arabidopsis F-box gene F-BOX STRESS INDUCED 1 (FBS1) for its effects on gene expression in order elucidate its previously unknown biological function...
July 17, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28715115/membrane-topology-and-identification-of-key-residues-of-eadact-a-plant-mboat-with-unusual-substrate-specificity
#12
Tam N T Tran, Jennifer Shelton, Susan Brown, Timothy P Durrett
Euonymus alatus diacylglycerol acetyltransferase (EaDAcT) catalyzes the transfer of an acetyl group from acetyl-CoA to the sn-3 position of diacylglycerol (DAG) to form 3-acetyl-1,2-diacyl-sn-glycerol (acetyl-TAG). EaDAcT belongs to a small, plant-specific subfamily of the membrane bound O-acyltransferases (MBOAT) that acylate different lipid substrates. Sucrose gradient density centrifugation revealed that EaDAcT colocalizes to the same fractions as an endoplasmic reticulum specific marker. By mapping the membrane topology of EaDAcT, we obtained an experimentally determined topology model for a plant MBOAT...
July 17, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28708375/enzyme-that-makes-you-cry-crystal-structure-of-lachrymatory-factor-synthase-from-allium-cepa
#13
Josie A Silvaroli, Matthew J Pleshinger, Surajit Banerjee, Philip D Kiser, Marcin Golczak
The biochemical pathway that gives onions their savor is part of the chemical warfare against microbes and animals. This defense mechanism involves formation of a volatile lachrymatory factor (LF) ((Z)-propanethial S-oxide) that causes familiar eye irritation associated with onion chopping. LF is produced in a reaction catalyzed by lachrymatory factor synthase (LFS). The principles by which LFS facilitates conversion of a sulfenic acid substrate into LF have been difficult to experimentally examine owing to the inherent substrate reactivity and lability of LF...
July 14, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28708000/reactive-oxygen-species-and-mitochondrial-homeostasis-as-regulators-of-stem-cell-fate-and-function
#14
Darren Qiancheng Tan, Toshio Suda
SIGNIFICANCE: The precise role and impact of reactive oxygen species (ROS) in stem cells, which are essential for lifelong tissue homeostasis and regeneration, remains of significant interest to the field. The long-term regenerative potential of a stem cell compartment is determined by the delicate balance between quiescence, self-renewal and differentiation; all of which can be influenced by ROS levels. Recent advances: The past decade has seen a growing appreciation for the importance of ROS and redox homeostasis in various stem cell compartments; particularly those of hematopoietic, neural and muscle tissues...
July 14, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28703789/capturing-suboptical-dynamic-structures-in-lipid-bilayer-patches-formed-from-free-standing-giant-unilamellar-vesicles
#15
Tripta Bhatia, Flemming Cornelius, John H Ipsen
There is accumulating evidence that the small-scale lateral organization of biological membranes has a crucial role in signaling and trafficking in cells. However, it has been difficult to characterize these features with existing methods for preparing and analyzing freestanding membranes, because the dynamics occurs below the optical resolution possible with these protocols. We have developed a protocol that permits the imaging of lipid nanodomains and lateral protein organization in membranes of giant unilamellar vesicles (GUVs)...
August 2017: Nature Protocols
https://www.readbyqxmd.com/read/28702990/the-relationship-of-amphipathic-beta-structures-in-the-beta-1-domain-of-apolipoprotein-b-to-the-properties-of-the-secreted-lipoprotein-particles-in-mca-rh7777-cells
#16
Medha Manchekar, Richa Kapil, Zhihuan Sun, Jere P Segrest, Nassrin Dashti
Our previous studies demonstrated that the first 1000 amino acid residues (the βα1 domain) of human apolipoprotein (apo) B-100, referred to as apoB:1000, are required for the initiation of lipoprotein assembly and the formation of a monodisperse stable phospholipid (PL)-rich particle. The objectives of this study were to: a) assess the effects on the properties of apoB truncates undergoing sequential inclusion of the amphipathic β strands in the N-terminal 700 residues of the β1 domain of apoB-100, and b) identify the subdomain in the β1 domain that is required for the formation of a microsomal triglyceride transfer protein (MTP)-dependent triacylglycerol (TAG)-rich apoB-containing particle...
July 13, 2017: Biochemistry
https://www.readbyqxmd.com/read/28700926/the-energetics-of-chromophore-binding-in-the-visual-photoreceptor-rhodopsin
#17
He Tian, Thomas P Sakmar, Thomas Huber
The visual photoreceptor rhodopsin is a prototypical G-protein-coupled receptor (GPCR) that stabilizes its inverse agonist ligand, 11-cis-retinal (11CR), by a covalent, protonated Schiff base linkage. In the visual dark adaptation, the fundamental molecular event after photobleaching of rhodopsin is the recombination reaction between its apoprotein opsin and 11CR. Here we present a detailed analysis of the kinetics and thermodynamics of this reaction, also known as the "regeneration reaction". We compared the regeneration of purified rhodopsin reconstituted into phospholipid/detergent bicelles with rhodopsin reconstituted into detergent micelles...
July 11, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28698362/structural-insights-into-lipoprotein-n-acylation-by-escherichia-coli-apolipoprotein-n-acyltransferase
#18
Cameron L Noland, Michele D Kattke, Jingyu Diao, Susan L Gloor, Homer Pantua, Mike Reichelt, Anand K Katakam, Donghong Yan, Jing Kang, Inna Zilberleyb, Min Xu, Sharookh B Kapadia, Jeremy M Murray
Gram-negative bacteria express a diverse array of lipoproteins that are essential for various aspects of cell growth and virulence, including nutrient uptake, signal transduction, adhesion, conjugation, sporulation, and outer membrane protein folding. Lipoprotein maturation requires the sequential activity of three enzymes that are embedded in the cytoplasmic membrane. First, phosphatidylglycerol:prolipoprotein diacylglyceryl transferase (Lgt) recognizes a conserved lipobox motif within the prolipoprotein signal sequence and catalyzes the addition of diacylglycerol to an invariant cysteine...
July 11, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28698208/plasma-lipoprotein-sub-fraction-concentrations-are-associated-with-lipid-metabolism-and-age-related-macular-degeneration
#19
Chui Ming Gemmy Cheung, Alfred Gan, Qiao Fan, Miao Ling Chee, Rajendra S Apte, Chiea Chuen Khor, Ian Yeo, Ranjana Mathur, Ching-Yu Cheng, Tien Yin Wong, E Shyong Tai
Disturbance in lipid metabolism has been suggested as a major pathogenic factor for age-related macular degeneration (AMD). Conventional lipid measures have been inconsistently associated with AMD. Other factors which can alter lipid metabolism include lipoprotein phenotype and genetic mutations. We performed a case-control study to examine the association between lipoprotein profile and neovascular AMD (nAMD), and whether the cholesterylester transfer protein CETP D442G mutation modulates these associations...
July 11, 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/28692330/regulation-of-cell-wall-genes-in-response-to-defective-kernel1-dek1-induced-cell-wall-changes
#20
Dhika Amanda, Monika S Doblin, Roberta Galletti, Antony Bacic, Gwyneth C Ingram, Kim L Johnson
Defective Kernel1 (DEK1) is a plant-specific calpain involved in epidermis specification and maintenance. DEK1 regulation of the epidermal cell wall is proposed to be key to ensure tissue integrity and coordinated growth. Changes in the expression of DEK1 are correlated with changes in the expression of cell wall-related genes. For example, we have found that Lipid transfer protein 3 (LTP3), EXPANSIN 11 (EXP11), and an AP2 transcription factor (AP2TF) are misexpressed in plants with constitutively altered levels of DEK1 activity...
July 10, 2017: Plant Signaling & Behavior
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