keyword
MENU ▼
Read by QxMD icon Read
search

Lipid transfer protein

keyword
https://www.readbyqxmd.com/read/29334397/recombinant-phosphatidylserine-binding-nanobodies-for-targeting-of-extracellular-vesicles-to-tumor-cells-a-plug-and-play-approach
#1
Sander A A Kooijmans, Jerney J J M Gitz-Francois, Raymond M Schiffelers, Pieter Vader
Extracellular vesicles (EVs) are increasingly being recognized as candidate drug delivery systems due to their ability to functionally transfer biological cargo between cells. However, manipulation of targeting properties of EVs through engineering of the producer cells can be challenging and time-consuming. As a novel approach to confer tumor targeting properties to isolated EVs, we generated recombinant fusion proteins of nanobodies against the epidermal growth factor receptor (EGFR) fused to phosphatidylserine (PS)-binding domains of lactadherin (C1C2)...
January 15, 2018: Nanoscale
https://www.readbyqxmd.com/read/29327816/brain-cortex-microglia-derived-exosomes-novel-nanoparticles-for-glioma-therapy
#2
Adriana-Natalia Murgoci, Dasa Cizkova, Petra Majerova, Eva Petrovova, Lubomir Medvecky, Isabelle Fournier, Michel Salzet
The function and integrity of nervous system require interactive exchanges among neurons and glial cells. Exosomes and other extracellular vesicles (EVs) are emerging as a key mediator of intercellular communication, capable to transfer nucleic acids, proteins and lipids, influencing numerous functional and pathological aspects of both donor and recipient cells. The immune response mediated by microglia derived exosomes are most prominently involved in the spread of neuroinflammation, neurodegenerative disorders and brain cancer...
January 12, 2018: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/29326160/hypoxia-inducible-lipid-droplet-associated-protein-inhibits-adipose-triglyceride-lipase
#3
Krishna M Padmanabha Das, Lisa Wechselberger, Márton Liziczai, Montserrat De la Rosa Rodriguez, Gernot F Grabner, Christoph Heier, Roland Viertlmayr, Claudia Radler, Joerg Lichtenegger, Robert Zimmermann, Jan Willem Borst, Rudolf Zechner, Sander Kersten, Monika Oberer
Elaborate control mechanisms of intracellular triacylglycerol (TAG) breakdown are critically involved in the maintenance of energy homeostasis. Hypoxia inducible lipid droplet associated protein (HILPDA)/Hypoxia inducible gene-2 (Hig-2) has been shown to affect intracellular TAG levels, yet, the underlying molecular mechanisms are unclear. Here, we show that HILPDA inhibits adipose triglyceride lipase (ATGL), the enzyme catalyzing the first step of intracellular TAG hydrolysis. HILPDA shares structural similarity with G0/G1 switch gene 2 (G0S2), an established inhibitor of ATGL...
January 11, 2018: Journal of Lipid Research
https://www.readbyqxmd.com/read/29311554/oxidation-of-atg3-and-atg7-mediates-inhibition-of-autophagy
#4
Karen Frudd, Thomas Burgoyne, Joseph Robert Burgoyne
Macroautophagy (autophagy) is a crucial cellular stress response for degrading defective macromolecules and organelles, as well as providing bioenergetic intermediates during hypoxia and nutrient deprivation. Here we report a thiol-dependent process that may account for impaired autophagy during aging. This is through direct oxidation of key autophagy-related (Atg) proteins Atg3 and Atg7. When inactive Atg3 and Atg7 are protected from oxidation due to stable covalent interaction with their substrate LC3. This interaction becomes transient upon activation of Atg3 and Atg7 due to transfer of LC3 to phosphatidylethanolamine (lipidation), a process crucial for functional autophagy...
January 8, 2018: Nature Communications
https://www.readbyqxmd.com/read/29305831/functional-evaluation-of-tryptophans-in-glycolipid-binding-and-membrane-interaction-by-hetc2-a-fungal-glycolipid-transfer-protein
#5
Roopa Kenoth, Xianqiong Zou, Dhirendra K Simanshu, Helen M Pike, Lucy Malinina, Dinshaw J Patel, Rhoderick E Brown, Ravi Kanth Kamlekar
HET-C2 is a fungal glycolipid transfer protein (GLTP) that uses an evolutionarily-modified GLTP-fold to achieve more focused transfer specificity for simple neutral glycosphingolipids than mammalian GLTPs. Only one of HET-C2's two Trp residues is topologically identical to the three Trp residues of mammalian GLTP. Here, we provide the first assessment of the functional roles of HET-C2 Trp residues in glycolipid binding and membrane interaction. Point mutants HET-C2W208F, HET-C2W208A and HET-C2F149Y all retained >90% activity and 80-90% intrinsic Trp fluorescence intensity; whereas HET-C2F149A transfer activity decreased to ~60% but displayed ~120% intrinsic Trp emission intensity...
January 3, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29305383/is-abca1-a-lipid-transfer-protein
#6
Michael C Phillips
ATP-binding cassette transporter A1 (ABCA1) functions as a lipid transporter because it mediates the transfer of cellular phospholipid (PL) and free (unesterified) cholesterol (FC) to apolipoprotein (apo) A-I and related proteins present in the extracellular medium. ABCA1 is a membrane PL translocase and its enzymatic activity leads to transfer of PL molecules from the cytoplasmic leaflet to the exofacial leaflet of a cell plasma membrane. The presence of active ABCA1 in the plasma membrane promotes binding of apoA-I to the cell surface...
January 5, 2018: Journal of Lipid Research
https://www.readbyqxmd.com/read/29301962/structure-of-the-yeast-oligosaccharyltransferase-complex-gives-insight-into-eukaryotic-n-glycosylation
#7
Rebekka Wild, Julia Kowal, Jillianne Eyring, Elsy M Ngwa, Markus Aebi, Kaspar P Locher
Oligosaccharyltransferase (OST) is an essential membrane protein complex in the Endoplasmic Reticulum, where it transfers an oligosaccharide from a dolichol-pyrophosphate-activated donor to glycosylation sites of secretory proteins. We here describe the atomic structure of yeast OST determined by cryo-EM, revealing a conserved subunit arrangement. The active site of the catalytic STT3 subunit points away from the center of the complex, allowing unhindered access to substrates. The dolichol-pyrophosphate moiety binds to a lipid-exposed groove of STT3, while two non-catalytic subunits and an ordered N-glycan form a membrane-proximal pocket for the oligosaccharide...
January 4, 2018: Science
https://www.readbyqxmd.com/read/29301859/parl-partitions-the-lipid-transfer-protein-stard7-between-the-cytosol-and-mitochondria
#8
Shotaro Saita, Takashi Tatsuta, Philipp A Lampe, Tim König, Yohsuke Ohba, Thomas Langer
Intramembrane-cleaving peptidases of the rhomboid family regulate diverse cellular processes that are critical for development and cell survival. The function of the rhomboid protease PARL in the mitochondrial inner membrane has been linked to mitophagy and apoptosis, but other regulatory functions are likely to exist. Here, we identify the START domain-containing protein STARD7 as an intramitochondrial lipid transfer protein for phosphatidylcholine. We demonstrate that PARL-mediated cleavage during mitochondrial import partitions STARD7 to the cytosol and the mitochondrial intermembrane space...
January 4, 2018: EMBO Journal
https://www.readbyqxmd.com/read/29299993/exosomes-in-cholesterol-metabolism-and-atherosclerosis
#9
Allison B Reiss, Nicholas A Vernice, Nicolle M Siegart, Joshua DeLeon, Lora J Kasselman
OBJECTIVE: Exosomes are small secreted membrane vesicles formed in the late endocytic compartments by inward budding. Interest in these extracellular vesicles and their role in atherosclerosis is growing, as they can affect multiple cellular processes that lead to lipid overload, cytokine secretion and cellular adhesion. Exosomes protect and transport lipids, proteins, and RNAs, fostering intercellular communication among different cell types involved in atherogenesis such as macrophages, endothelium and smooth muscle...
January 3, 2018: Cardiovascular & Hematological Disorders Drug Targets
https://www.readbyqxmd.com/read/29290805/exosome-theranostics-biology-and-translational-medicine
#10
REVIEW
Chuanjiang He, Shu Zheng, Yan Luo, Ben Wang
Exosomes are common membrane-bound nanovesicles that contain diverse biomolecules, such as lipids, proteins, and nucleic acids. Exosomes are derived from cells through exocytosis, are ingested by target cells, and can transfer biological signals between local or distant cells. Exosome secretion is a constitutive phenomenon that is involved in both physiological and pathological processes and determines both the exosomal surface molecules and the contents. Hence, we can exploit exosomes as biomarkers, vaccines and drug carriers and modify them rationally for therapeutic interventions...
2018: Theranostics
https://www.readbyqxmd.com/read/29289533/ldl-receptor-gene-ablated-hamsters-a-rodent-model-of-familial-hypercholesterolemia-with-dominant-inheritance-and-diet-induced-coronary-atherosclerosis
#11
Xin Guo, Mingming Gao, Yunan Wang, Xiao Lin, Liu Yang, Nathan Cong, Xiangbo An, Feng Wang, Kai Qu, Liqing Yu, Yuhui Wang, Jinjie Wang, Haibo Zhu, Xunde Xian, George Liu
Familial hypercholesterolemia (FH) is an autosomal dominant genetic disease caused mainly by LDL receptor (Ldlr) gene mutations. Unlike FH patients, heterozygous Ldlr knockout (KO) mice do not show a dominant FH trait. Hamsters, like humans, have the cholesteryl ester transfer protein, intestine-only ApoB editing and low hepatic cholesterol synthesis. Here, we generated Ldlr-ablated hamsters using CRISPR/Cas9 technology. Homozygous Ldlr KO hamsters on a chow diet developed hypercholesterolemia with LDL as the dominant lipoprotein and spontaneous atherosclerosis...
December 15, 2017: EBioMedicine
https://www.readbyqxmd.com/read/29287690/implication-of-stard5-and-cholesterol-homeostasis-disturbance-in-the-endoplasmic-reticulum-stress-related-response-induced-by-pro-apoptotic-aminosteroid-rm-133
#12
Martin Perreault, René Maltais, Lucie-Carole Kenmogne, Danny Létourneau, Jean-Guy LeHoux, Stéphane Gobeil, Donald Poirier
The aminosteroid derivative RM-133 is an effective anticancer molecule for which proof of concept has been achieved in several mouse xenograph models (HL-60, MCF-7, PANC-1 and OVCAR-3). To promote this new family of molecules toward a clinical phase 1 trial, the mechanism of action governing the anticancer properties of the representative candidate RM-133 needs to be characterized. In vitro experiments were first used to determine that RM-133 causes apoptosis in cancer cells. Then, using proteomic and transcriptomic experiments, RM-133 cytotoxicity was proven to be achieved via the endoplasmic reticulum (ER)-related apoptosis, which characterizes RM-133 as an endoplasmic reticulum stress aggravator (ERSA) anticancer drug...
December 26, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/29284607/weight-loss-and-exercise-alter-the-high-density-lipoprotein-lipidome-and-improve-high-density-lipoprotein-functionality-in-metabolic-syndrome
#13
Anmar A Khan, Piyushkumar A Mundra, Nora E Straznicky, Paul J Nestel, Gerard Wong, Ricardo Tan, Kevin Huynh, Theodore W Ng, Natalie A Mellett, Jacquelyn M Weir, Christopher K Barlow, Zahir H Alshehry, Gavin W Lambert, Bronwyn A Kingwell, Peter J Meikle
OBJECTIVE: High-density lipoprotein (HDL) lipid composition and function may better reflect cardiovascular risk than HDL cholesterol concentration. This study characterized the relationships between HDL composition, metabolism, and function in metabolic syndrome (MetS) patients and how changes in composition after weight loss (WL) and exercise treatments are related to function. APPROACH AND RESULTS: Plasma samples from MetS patients (n=95) and healthy individuals (n=40) were used in this study...
December 28, 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/29282861/biological-functions-and-current-advances-in-isolation-and-detection-strategies-for-exosome-nanovesicles
#14
REVIEW
Kseniia Boriachek, Md Nazmul Islam, Andreas Möller, Carlos Salomon, Nam-Trung Nguyen, Md Shahriar A Hossain, Yusuke Yamauchi, Muhammad J A Shiddiky
Exosomes are nanoscale (≈30-150 nm) extracellular vesicles of endocytic origin that are shed by most types of cells and circulate in bodily fluids. Exosomes carry a specific composition of proteins, lipids, RNA, and DNA and can work as cargo to transfer this information to recipient cells. Recent studies on exosomes have shown that they play an important role in various biological processes, such as intercellular signaling, coagulation, inflammation, and cellular homeostasis. These functional roles are attributed to their ability to transfer RNA, proteins, enzymes, and lipids, thereby affecting the physiological and pathological conditions in various diseases, including cancer and neurodegenerative, infectious, and autoimmune diseases (e...
December 28, 2017: Small
https://www.readbyqxmd.com/read/29280647/-relation-of-variant-rs180077-of-gen-cholesterol-ester-transfer-protein-variant-with-fat-mass-hdl-cholesterol-in-obese-subjects-with-diabetes-mellitus-type-2
#15
Daniel Antonio De Luis, Olatz Izaola, David Primo, Susana García Calvo, Emilia Gómez Hoyos, Juan José López Gómez, Ana Ortola, Cristina Serrano, Esther Delgado, Beatriz Torres Torres
BACKGROUND: There is few evidence of cholesterol ester transfer protein (CETP) in subjects with obesity and diabetes mellitus. OBJECTIVES: We examined the association of the polymorphism (rs1800777) of CETP gene on anthropometric parameters, lipid profile and adipokines in subjects with obesity and diabetes mellitus type 2. MATERIAL AND METHODS: A population of 229 obese subjects with diabetes mellitus type 2 was enrolled. An electrical bioimpedance, blood pressure, dietary intake, exercise and biochemical analyses were recorded...
November 14, 2017: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
https://www.readbyqxmd.com/read/29280621/study-of-fret-to-lipid-domain-markers-ascertains-partitioning-of-semisynthetic-lipidated-n-ras-in-lipid-raft-nanodomains
#16
Anna K Shishina, Elizaveta A Kovrigina, Azamat R Galiakhmetov, Rajendra Rathore, Evgenii L Kovrigin
Cellular membranes are heterogeneous planar lipid bilayers displaying lateral phase separation with the nanometer-scale liquid-ordered phase (aka "lipid rafts") surrounded by the liquid-disordered phase. Many membrane-associated proteins were found to permanently integrate in the lipid rafts, which is critical for their biological function. Isoforms H and N of Ras GTPase possess a unique ability to switch their lipid domain preference depending on the type of the bound guanine nucleotide (GDP or GTP). This behavior, however, has never been demonstrated in vitro in model bilayers with recombinant proteins, and therefore has been attributed to the action of Ras binding to other proteins at the membrane surface...
December 27, 2017: Biochemistry
https://www.readbyqxmd.com/read/29279437/-the-biological-role-of-exosomes-in-bone-remodeling-and-bone-diseases
#17
Fumihiko Urabe, Yusuke Yoshioka, Takahiro Ochiya
Exosomes are about 100nm membrane vesicles, and released from almost all cell types. They carry and transfer a wide variety of molecules, such as mRNAs, microRNAs, proteins, and lipids, as modulators of intercellular communication. Various studies have shown that this exosome-mediated intercellular communication lead to proliferation, invasion and metastasis of cancer cells. In addition to that, emerging data suggest that exosomes are also involved in physiological processes of bone remodeling and bone diseases...
2018: Clinical Calcium
https://www.readbyqxmd.com/read/29279306/structure-function-insights-into-direct-lipid-transfer-between-membranes-by-mmm1-mdm12-of-ermes
#18
Shin Kawano, Yasushi Tamura, Rieko Kojima, Siqin Bala, Eri Asai, Agnès H Michel, Benoît Kornmann, Isabelle Riezman, Howard Riezman, Yoshitake Sakae, Yuko Okamoto, Toshiya Endo
The endoplasmic reticulum (ER)-mitochondrial encounter structure (ERMES) physically links the membranes of the ER and mitochondria in yeast. Although the ER and mitochondria cooperate to synthesize glycerophospholipids, whether ERMES directly facilitates the lipid exchange between the two organelles remains controversial. Here, we compared the x-ray structures of an ERMES subunit Mdm12 from Kluyveromyces lactis with that of Mdm12 from Saccharomyces cerevisiae and found that both Mdm12 proteins possess a hydrophobic pocket for phospholipid binding...
December 26, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/29276178/the-cdc50a-extracellular-domain-is-required-for-forming-a-functional-complex-with-and-chaperoning-phospholipid-flippases-to-the-plasma-membrane
#19
Katumori Segawa, Sachiko Kurata, Shigekazu Nagata
Flippases are enzymes that translocate phosphatidylserine (PtdSer) and phosphatidyl- ethanolamine (PtdEtn) from the outer to the inner leaflet in the lipid bilayer of the plasma membrane, leading to the asymmetric distribution of aminophospholipids in the membrane. One mammalian phospholipid flippase at the plasma membrane is ATP11C, a type IV P-type ATPase (P4-ATPase) that forms a hetero-complex with the transmembrane protein CDC50A. However, the structural features in CDC50A that support the function of ATP11C and other P4-ATPases have not been characterized...
December 24, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29274338/structural-insights-into-a-start-like-domain-in-lam4-and-its-interaction-with-sterol-ligands
#20
Alberto T Gatta, Andrea C Sauerwein, Anastasia Zhuravleva, Tim P Levine, Stephen Matthews
Sterols are essential components of cellular membranes and shape their biophysical properties. The recently discovered family of Lipid transfer proteins Anchored at Membrane contact sites (LAMs) has been suggested to carry out intracellular sterol traffic using StART-like domains. Here, we studied the second StART-like domain of Lam4p from S. cerevisiae by NMR. We show that NMR data are consistent with the StART-like domain structure, and that several functionally important regions within the domain exhibit significant conformational dynamics...
December 20, 2017: Biochemical and Biophysical Research Communications
keyword
keyword
75579
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"