Naoko Watanabe-Okochi, Ayaka Sato, Akira Okuyama, Go Tomiyoshi, Yumi Suzuki, Yukiko Watanabe, Kaori Kitsukawa, Masako Anazawa, Takashige Shimoyamada, Daisuke Takahashi, Takayuki Onodera, Makoto Uchikawa, Nelson-Hirokazu Tsuno, Kazuo Muroi
BACKGROUND AND OBJECTIVES: In Japan, the prevalence of haptoglobin deficiency is approximately 1 in 4000. Haptoglobin-deficient individuals may produce anti-haptoglobin from allo-immunization, leading to serious transfusion reactions. Therefore, implementation of a consistent supply of haptoglobin-deficient fresh frozen plasma is crucial. We developed a novel reagent to facilitate large-scale identification of haptoglobin-deficient individuals as potential donors of plasma products. MATERIALS AND METHODS: We established mouse monoclonal anti-haptoglobin-producing cell lines (three clones) using the hybridoma method by immunizing mice with the haptoglobin protein...
October 5, 2023: Vox Sanguinis