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https://www.readbyqxmd.com/read/29046057/bile-duct-ligature-in-young-rats-a-revisited-animal-model-for-biliary-atresia
#1
Matias Garrido, Camila Escobar, Constanza Zamora, Carolina Rejas, Juan Varas, Mario Párraga, Sebastian San Martin, Sandra Montedónico
Biliary atresia leads to cirrhosis in the vast majority of patients and constitutes the first cause of paediatric liver transplantation. Animal models allow us to understand the molecular basis and natural history of diseases. The aim of this study is to describe a surgically created animal model of biliary atresia with emphasis in long-term liver function. Forty-two 3-week-old Sprague-Dawley rats were randomly divided into two groups: bile duct ligature (BDL) and control. The animals were sacrificed on the 2nd, 4th, and 6th postoperative weeks...
September 13, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/29046052/protective-effect-of-different-antioxidant-agents-in-uvb-irradiated-keratinocytes
#2
Sara Salucci, Sabrina Burattini, Francesca Buontempo, Alberto Maria Martelli, Elisabetta Falcieri, Michela Battistelli
Skin cells can respond to UVB-induced damage either by tolerating it, or restoring it through antioxidant activation and DNA repair mechanisms or, ultimately, undergoing programmed cell death, when damage is massive. Nutritional factors, in particular, food antioxidants, have attracted much interest because of their potential use in new preventive, protective, and therapeutic strategies for chronic degenerative diseases, including skin inflammation and cancer. Some polyphenols, present in virgin olive oil, well tolerated by organism after oral administration, show a variety of pharmacological and clinical benefits such as anti-oxidant, anti-cancer, anti-inflammatory, and neuro-protective activities...
September 18, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/29046046/three-dimensional-evaluation-of-similarity-of-right-and-left-knee-joints
#3
Ki-Mo Jang, Jong-Hoon Park, Minho Chang, Youngjun Kim, Deukhee Lee, Sehyung Park, Joon Ho Wang
Purpose: The purpose of this study was to evaluate the anatomical similarity of three-dimensional (3D) morphometric parameters between right and left knees. Materials and Methods: Ten fresh-frozen paired cadaveric knees were tested. Following dissection, footprint areas of the anterior cruciate ligament (ACL) and posterior cruciate ligament (PCL) were measured. Surface scanning was performed using a 3D scanner. Scanned data were reproduced and morphometric parameters were measured on specialized software...
October 19, 2017: Knee Surgery & related Research
https://www.readbyqxmd.com/read/29046027/-frequency-of-skin-reactivity-to-food-allergens-in-allergic-patients
#4
Heriberto Camero-Martínez, Aída Inés López-García, Daniela Rivero-Yeverino, Chrystopherson Gengyny Caballero-López, Oswaldo Arana-Muñoz, Sergio Papaqui-Tapia, Isabel Cristina Rojas-Méndez, Elizabeth Vázquez-Rojas
BACKGROUND: Food allergy is deemed to have a worldwide prevalence ranging from 2 to 10 %. OBJECTIVE: To determine the frequency of skin reactivity to food allergens by age groups. METHODS: Cross-sectional, descriptive, prolective, observational study. Patients aged from 2 to 64 years with symptoms consistent with allergic disease were included. Skin prick tests were carried out with food allergens. Frequencies and percentages were estimated...
July 2017: Revista Alergia Mexico: Organo Oficial de la Sociedad Mexicana de Alergia e Inmunología, A.C
https://www.readbyqxmd.com/read/29046014/tomato-cry1a-plays-a-critical-role-in-the-regulation-of-phytohormone-homeostasis-plant-development-and-carotenoid-metabolism-in-fruits
#5
Chao-Chao Liu, Golam Jalal Ahammed, Guo-Ting Wang, Chang-Jie Xu, Kun-Song Chen, Yan-Hong Zhou, Jing-Quan Yu
Blue light photoreceptors, cryptochromes (CRYs), regulate multiple aspects of plant growth and development. However, our knowledge of CRYs is predominantly based on model plant Arabidopsis at early growth stage. In this study, we elucidated functions of CRY1a gene in mature tomato (Solanum lycopersicum) plants by using cry1a mutants and CRY1a overexpressing lines (OE-CRY1a-1 and OE-CRY1a-2). In comparison with wild-type plants, cry1a mutants are relatively tall, accumulate low biomass and bear more fruits, whereas OE-CRY1a plants are short stature, and they not only flower lately but also bear less fruits...
October 18, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/29046009/-experiences-of-patients-with-invasive-and-non-invasive-home-mechanical-ventilation-a-systematic-review-of-qualitative-research
#6
V Nelißen, S Metzing, W Schnepp
Aim of the study Human beings can be ventilated outside of a hospital after a lung failure or due to a neurodegenerative disease. This can be done in different living conditions. This article examines the question of what experiences such patients have made with invasive home mechanical ventilation, and how they have adapted their new lives. Methodology On the basis of a systematic literature research, qualitative studies were analyzed that depict the social reality of patients with invasive and non-invasive home mechanical ventilation...
October 18, 2017: Pneumologie
https://www.readbyqxmd.com/read/29045990/electronic-health-record-impacts-on-family-medicine-teachers-survey-of-third-year-medical-student-clerkship-preceptors-at-an-academic-medical-center
#7
Elizabeth Curry, Tamara K Oser, Sean M Oser
BACKGROUND AND OBJECTIVES: Electronic Health Record (EHR) use in clinical practice has accelerated in recent years. While several aspects of EHR use have been extensively studied, there is little data on EHR impacts on medical student educators, especially those involved in outpatient family medicine. This study evaluated perceived impacts of EHR use on clinician teachers of outpatient family medicine. METHODS: The study used a mixed methods survey of clinicians who teach third-year medical students during the required family and community medicine outpatient clerkship at a Mid-Atlantic medical school...
October 2017: Family Medicine
https://www.readbyqxmd.com/read/29045984/results-of-the-2017-national-resident-matching-program%C3%A2-and-the-american-osteopathic-association-intern-resident-registration-program
#8
Stanley M Kozakowski, Alexandra Travis, Julie P Marcinek, Ashley Bentley, Gerald T Fetter
The purpose of medicine as a profession is to meet the health needs of people and communities. Despite empirical evidence worldwide that an appropriate foundation of primary care in a health care system leads to improved health outcomes, improved experience of health care, a reduction in health disparities, and lower overall cost of care, publicly available data from National Resident Matching Program® (NRMP) and the American Osteopathic Association (AOA) Intern/Resident Registration Program show that PGY-1 family medicine and primary care positions offered in the NRMP Match continue to grow, but are losing ground in comparison to the growth of non-primary care specialties...
October 2017: Family Medicine
https://www.readbyqxmd.com/read/29045976/-anatomy-and-histology-characteristics-of-lymph-node-in-nude-mice
#9
R Sun, B Gao, C B Guo
OBJECTIVE: To compare the differences of anatomical and histological characteristics of lymph nodes between BALB/c nude mice and BALB/c mice. METHODS: Firstly, twenty BALB/c nude mice and twenty BALB/c mice were dissected by using a surgical microscope. Secondly, the differences of T cells and B cells at the lymph node were compared by the expressions of CD3 and CD20 immunohistochemistry dyes. RESULTS: There were, on average, 23 nodes per mouse contained within the large lymph node assembly in the BALB/c nude mouse...
October 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/29045966/-immune-effects-of-specific-ctls-response-induced-by-dendritic-cells-pulsed-with-ny-eso-1-peptide
#10
J W Liu, X Lu, Z M Yang, L J Deng, L Yang
OBJECTIVE: To explore the potential of autologous dendritic cells (DCs) pulsed with caner/testis antigen NY-ESO-1 peptides in inducing specific cytotoxic T lymphocyte (CTLs) response and antineoplastic immune function of specific CTLs. METHODS: Fifteen patients with II to III stage positive HLA -A0201(+) and NY-ESO-1(+) were enrolled in the Cancer Hospital Chinese Academy of Medical Sciences on the basis of preclinical experiments from November 2014 to October 2015, and their peripheral blood mononuclear cells (PBMCs) and peripheral blood lymphocytes (PBLs) were isolated...
October 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/29045964/-increased-serum-c-c-chemokine-ligand-19-levels-correlated-with-b-cell-abnormalities-in-systemic-lupus-erythematosus
#11
H J Liu, L J Shi, F L Hu, H H Yao, Z G Li, Y Jia
OBJECTIVE: To detect the levels of serum C-C chemokine ligand 19 (CCL19) in patients with systemic lupus erythematosus (SLE) and to evaluate the correlation between CCL19 expression and clinical features and laboratory parameters, trying to reveal the possible role of CCL19 in the pathogenesis of systemic lupus erythematosus. METHODS: The levels of serum CCL19 were measured by enzyme linked immunosorbent assay (ELISA) in 90 patients with SLE and 30 healthy controls...
October 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/29045963/-tumor-derived-igg-suppress-the-proliferation-of-t-cells-in-cord-blood
#12
E Y Liu, J F Liu, W W Shao, L Xiao, G H Li, X H Chang, X Y Qiu
OBJECTIVE: To explore the function of tumor derived IgG (tIgG) and whether the tIgG can inhibit T cells activity. METHODS: The tIgG was purified from ovarian cancer tissue. The cord blood monocyte cells (CBMC) and cord blood lymphocyte (CBL) were isolate from human umbilical cord blood. The CBMC and CBL were stimulated with phytohaemagg lutinin (PHA) in order to let the CBMC and CBL in the state of proliferation. Carboxyfluorescein succinimidyl amino ester (CFSE) was cultured with CBMC and CBL...
October 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/29045962/-myeloid-derived-suppressor-cells-promoted-autologous-b-cell-proliferation-in-rheumatoid-arthritis
#13
W Chen, F L Hu, H J Liu, L L Xu, Y N Li, Z G Li
OBJECTIVE: To investigate the effect of myeloid-derived suppressor cells (MDSC) on pro-liferation of B lymphocytes in rheumatoid arthritis (RA) patients. METHODS: The peripheral blood specimens were collected from 15 healthy adults and 38 RA patients who were divided into high disease activity group, medium activity group and low activity group according to their 28-joint disease activity score (DAS28). And the frequencies of MDSC were determined by flow cytometry...
October 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/29045955/-comparison-between-continuous-noninvasive-hemoglobin-monitoring-and-venous-blood-hemoglobin-monitoring-in-children-with-kidney-disease
#14
X Y Liu, X Q Yang, H J Xiao, J Ding
OBJECTIVE: To assess the correlation and consistency between continuous noninvasive hemoglobin detection and venous blood hemoglobin detection in children with kidney disease, and try to analyze the affecting factors. Try to provide a reference for the monitoring of hemoglobin in children with kidney disease by continuous noninvasive hemoglobin detection technique. METHODS: Eighty-five inpatient children with kidney disease, 50 boys (58.8%) and 35 girls (41.2%), aged from 3 years old to 18 years old (9...
October 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/29045953/-detection-of-large-deletions-in-x-linked-alport-syndrome-using-competitive-multiplex-fluorescence-polymerase-chain-reaction
#15
F Wang, Y Q Zhang, J Ding, L X Yu
OBJECTIVE: To evaluate the ability of multiplex competitive fluorescence polymerase chain reaction in detection of large deletion and duplication genotypes of X-linked Alport syndrome. METHODS: Clinical diagnosis of X-linked Alport syndrome was based on either abnormal staining of type IV collagen α5 chain in the epidermal basement membrane alone or with abnormal staining of type IV collagen α5 chain in the glomerular basement membrane and Bowman's capsule/ultrastructural changes in the glomerular basement membrane typical of Alport syndrome...
October 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/29045942/quantitative-renal-echogenicity-as-a-tool-for-diagnosis-of-advanced-chronic-kidney-disease-in-patients-with-glomerulopathies-and-no-liver-disease
#16
Alexandre B Libório, Fernanda Macedo de Oliveira Neves, Candice Bezerra Torres de Melo, Tacyano Tavares Leite, Renata de Almeida Leitão
BACKGROUND/AIMS: Glomerulopathy patients are prone to developing transitory reduced glomerular filtration rate (GFR), which can be difficult to differentiate from irreversible chronic kidney disease (CKD). Renal ultrasound can be useful, but differently from renal length, quantitative renal echogenicity has not been formerly evaluated regarding its capacity to identify irreversible advanced CKD. METHODS: A prospective study was performed, where quantitative renal echogenicity was performed during renal biopsy in patients with suspected glomerular disease (n=197)...
October 18, 2017: Kidney & Blood Pressure Research
https://www.readbyqxmd.com/read/29045930/label-free-aptamer-based-sensor-for-specific-detection-of-malathion-residues-by-surface-enhanced-raman-scattering
#17
Yonghui Nie, Yuanjie Teng, Pan Li, Wenhan Liu, Qianwei Shi, Yuchao Zhang
A novel label-free aptamer surface-enhanced Raman scattering (SERS) sensor for trace malathion residue detection was proposed. In this process, the binding of malathion molecule with aptamer is identified directly. The silver nanoparticles modified with positively charged spermine served as enhancing and capture reagents for the negatively charged aptamer. Then, the silver nanoparticles modified by aptamer were used to specifically capture the malathion. The SERS background spectra of spermine, aptamer, and malathion were recorded and distinguished with the spectrum of malathion-aptamer...
October 10, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29045916/suicide-deaths-among-patients-with-end-stage-renal-disease-receiving-dialysis-a-population-based-retrospective-cohort-study-of-64-000-patients-in-taiwan
#18
I-Ming Chen, Po-Hsien Lin, Vin-Cent Wu, Chi-Shin Wu, Jia-Chi Shan, Shu-Sen Chang, Shih-Cheng Liao
BACKGROUND: Patients with end-stage renal disease (ESRD) who receive dialysis may experience increased distress and risk of suicide. METHODS: This population-based retrospective cohort study linked Taiwan's national register of ESRD patients on dialysis and the cause-of-death mortality data file. A separate multiple-cause-of-death data file was used to investigate the detailed suicide methods used. Standardized mortality ratios (SMRs) were calculated for the overall patient group and by sex, age, year of initiating dialysis, method of suicide, and time since initiation of dialysis...
October 9, 2017: Journal of Affective Disorders
https://www.readbyqxmd.com/read/29045912/the-lived-experience-of-first-year-undergraduate-student-nurses-a-hermeneutic-phenomenological-study
#19
Debra J Porteous, Alison Machin
INTRODUCTION: This study gives insight into the experiences and perceptions of one group of undergraduate nursing students as they make the transition into Higher Education and the nursing profession, during the first year, of their three-year programme. Research has shown that first year undergraduate experience is complex and challenging for any student. For undergraduate nursing students, the process of achieving additional professional practice competencies required for United Kingdom nursing registration adds additional responsibility and potentially, more pressure...
October 3, 2017: Nurse Education Today
https://www.readbyqxmd.com/read/29045909/an-integrative-review-of-graduate-transition-programmes-developmental-considerations-for-nursing-management
#20
REVIEW
Shannon Bakon, Judy Craft, Lisa Wirihana, Martin Christensen, Jennie Barr, Lily Tsai
Graduate transition programmes have been developed to recruit new nursing staff and facilitate an effective transition from nursing student to Registered Nurse within the clinical environment. Therefore the aim of this paper was to explore the various elements included in nursing graduate transition programmes. An integrative review was undertaken incorporating a strict inclusion criterion, critical appraisal, and thematic analysis of 30 studies. There are numerous transition programmes available yet there remains a lack of transparency regarding their aims/objectives, course content, support timeframe and the type of support provided...
October 10, 2017: Nurse Education in Practice
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