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https://www.readbyqxmd.com/read/28330283/multiplex-pcr-for-simultaneous-identification-of-e-coli-o157-h7-salmonella-spp-and-l-monocytogenes-in-food
#1
Thuy Trang Nguyen, Vo Van Giau, Tuong Kha Vo
The rapid detection of pathogens in food is becoming increasingly critical for ensuring the safety of consumers, since the majority of food-borne illnesses and deaths are caused by pathogenic bacteria. Hence, rapid, sensitive, inexpensive and convenient approaches to detect food-borne pathogenic bacteria is essential in controlling food safety. In this study, a multiplex PCR assay for the rapid and simultaneous detection of Escherichia coli O157:H7, Salmonella spp. and Listeria monocytogenes was established...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330247/production-of-glucosinolates-phenolic-compounds-and-associated-gene-expression-profiles-of-hairy-root-cultures-in-turnip-brassica-rapa-ssp-rapa
#2
Ill-Min Chung, Kaliyaperumal Rekha, Govindasamy Rajakumar, Muthu Thiruvengadam
Turnip (Brassica rapa ssp. rapa) is an important vegetable crop producing glucosinolates (GSLs) and phenolic compounds. The GSLs, phenolic compound contents and transcript levels in hairy root cultures, as well as their antioxidant, antimicrobial and anticancer activity were studied in turnip. Transgenic hairy root lines were confirmed by polymerase chain reaction (PCR) and reverse transcription-PCR. GSLs levels (glucoallysin, glucobrassicanapin, gluconasturtiin, glucobrassicin, 4-methoxyglucobrassicin, neoglucobrassicin and 4-hydroxyglucobrassicin) and their gene expression levels (BrMYB28, BrMYB29, BrMYB34, BrMYB51, BrMYB122, CYP79 and CYP83) significantly increased in hairy roots compared with that in non-transformed roots...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28329822/it-s-all-about-the-relationship-cognitively-intact-mother-daughter-care-dyads-in-hospice-at-home
#3
Diane N Solomon, Lissi Hansen, Judith G Baggs
Purpose of the Study: Adult daughters providing care to aging, ill mothers comprise the most prevalent caregiving dyad. Little is known, however, regarding relationship quality and its impact on care in these dyads, particularly in the context of cognitively intact patients at end of life in hospice. This interpretive descriptive work privileges voices of terminally ill mothers and care-partnering daughters in the home hospice context. Specific aims were to describe and interpret how mothers and daughters: (a) perceive relationship quality and (b) perceive how relationships have developed over time through health, chronic illness, and hospice...
March 10, 2017: Gerontologist
https://www.readbyqxmd.com/read/28329734/elevated-mif-2-levels-predict-mortality-in-critically-ill-patients
#4
Julia Pohl, Ulrike B Hendgen-Cotta, Pia Stock, Peter Luedike, Tienush Rassaf
PURPOSE: D-dopachrome tautomerase (MIF-2 or DDT) is a member of the macrophage migration inhibitory factor (MIF) superfamily and a close structural homolog to MIF. Circulating MIF-2 has been described to be elevated in patients suffering from sepsis, severe burn injury and after surgery. We sought to evaluate the prognostic value of MIF-2 in critically ill patients. METHODS: A total of 72 patients were studied upon admission to the medical intensive care unit (ICU)...
March 16, 2017: Journal of Critical Care
https://www.readbyqxmd.com/read/28329715/psychological-distress-and-prejudice-following-terror-attacks-in-france
#5
Robin Goodwin, Krzysztof Kaniasty, Shaojing Sun, Menachem Ben-Ezra
Terrorist attacks have the capacity to threaten our beliefs about the world, cause distress across populations and promote discrimination towards particular groups. We examined the impact of two different types of attacks in the same city and same year on psychological distress and probable posttraumatic stress symptoms, and the moderating effects of religion or media use on distress/posttraumatic symptoms and inter-group relations. Two panel surveys four weeks after the January 2015 Charlie Hebdo attack (N = 1981) and the November 2015 Bataclan concert hall/restaurant attacks (N = 1878), measured intrinsic religiosity, social and traditional media use, psychological distress (K6), probable posttraumatic stress symptoms (proposed ICD-11), symbolic racism and willingness to interact with Muslims by non-Muslims...
March 16, 2017: Journal of Psychiatric Research
https://www.readbyqxmd.com/read/28329651/infections-in-children-with-eczema-in-uk-general-practice-a-cohort-study
#6
Sinéad M Langan, Katrina Abuabara, Sarah E Henrickson, Ole Hoffstad, David J Margolis
BackgroundCase reports and cross-sectional studies suggest increased risks of cutaneous and non-cutaneous infections in eczema; this relationship needs to be assessed in large population-based studies with diagnostic confirmation. The objective was to examine associations between eczema and common childhood infections.MethodsIndividuals registered prior to age 18 in the Health Improvement Network, a UK general practice database, from 2003 to 2013 were included in this cohort study. We determined the association between eczema and selected infectious outcomes, including cutaneous (dermatophyte, herpes simplex virus, impetigo, molluscum contagiosum and warts), and non-cutaneous infections (otitis media, streptococcal throat infections, and pneumonia)...
September 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329428/the-diagnosis-and-lived-experience-of-polycystic-ovary-syndrome-a-qualitative-study
#7
Julie Tomlinson, Jonathan Pinkney, Linda Adams, Elizabeth Stenhouse, Alison Bendall, Oonagh Corrigan, Gayle Letherby
AIMS: To explore the impact of the diagnosis of polycystic ovary syndrome on health/ill health identity, how women experience this diagnosis and their health beliefs. BACKGROUND: polycystic ovary syndrome is a common and heterogeneous condition, giving rise to a wide range of different health concerns. Previous research on polycystic ovary syndrome has been dominated by the medical perspective and less is known about the experiences and needs of women. DESIGN: A qualitative study of 32 premenopausal adult women with polycystic ovary syndrome (diagnosis confirmed by Rotterdam criteria), aged between 18-45 years, recruited from a primary and secondary care setting...
March 22, 2017: Journal of Advanced Nursing
https://www.readbyqxmd.com/read/28329417/nursing-judgement-and-decision-making-using-the-sedation-withdrawal-score-sws-in-children
#8
Jennie Craske, Bernie Carter, Ian H Jarman, Lyvonne N Tume
AIMS: The aim of the study was to evaluate registered children's nurses' approaches to the assessment and management of withdrawal syndrome in children. BACKGROUND: Assessment of withdrawal syndrome is undertaken following critical illness when the child's condition may be unstable with competing differential diagnoses. Assessment tools aim to standardise and improve recognition of withdrawal syndrome. Making the right decisions in complex clinical situations requires a degree of mental effort and it is not known how nurses make decisions when undertaking withdrawal assessments...
March 22, 2017: Journal of Advanced Nursing
https://www.readbyqxmd.com/read/28329388/healthcare-claims-data-an-underutilized-tool-for-pediatric-outpatient-antimicrobial-stewardship
#9
Joshua R Watson, Ling Wang, Jennifer Klima, Melissa Moore-Clingenpeel, Sean Gleeson, Kelly Kelleher, Preeti Jaggi
Background: Healthcare claims are under-utilized to identify factors associated with high outpatient antibiotic use. Methods: We evaluated ambulatory encounter claims of Medicaid-insured children in 34 Ohio counties in 2014. 1) Rates of total antibiotic and azithromycin prescriptions dispensed were determined by county of patient residence. 2) Standardized treatment rates by county were estimated for uncomplicated upper respiratory tract encounters (acute otitis media, pharyngitis, sinusitis, presumed viral infection) after adjusting for patient age and encounter provider type...
March 9, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28329331/natural-history-of-moderately-dilated-tubular-ascending-aorta-implications-for-determining-the-optimal-imaging-interval%C3%A2
#10
Kay-Hyun Park, Suryeun Chung, Dong Jung Kim, Jun Sung Kim, Cheong Lim
OBJECTIVES: For a moderately dilated ascending aorta (diameter 35-54 mm), current guidelines recommend continuous annual or semi-annual examinations with computed tomography or magnetic resonance imaging. However, few data have shown the yield and benefit of such a protocol. This study aimed to investigate the fate of a moderately dilated ascending aorta and thereby determine the adequate imaging interval. METHODS: In our institutional database, we identified adult patients having an ascending aortic diameter ≥40 mm in contrast-enhanced computed tomography and follow-up imaging(s) after ≥1 year...
February 20, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28329311/comparative-effectiveness-of-high-dose-versus-standard-dose-influenza-vaccines-among-us-medicare-beneficiaries-in-preventing-postinfluenza-deaths-during-2012-2013-and-2013-2014
#11
David K Shay, Yoganand Chillarige, Jeffrey Kelman, Richard A Forshee, Ivo M Foppa, Michael Wernecke, Yun Lu, Jill M Ferdinands, Arjun Iyengar, Alicia M Fry, Chris Worrall, Hector S Izurieta
Background.: Recipients of high-dose vs standard-dose influenza vaccines have fewer influenza illnesses. We evaluated the comparative effectiveness of high-dose vaccine in preventing postinfluenza deaths during 2012-2013 and 2013-2014, when influenza viruses and vaccines were similar. Methods.: We identified Medicare beneficiaries aged ≥65 years who received high-dose or standard-dose vaccines in community-located pharmacies offering both vaccines. The primary outcome was death in the 30 days following an inpatient or emergency department encounter listing an influenza International of Classification of Diseases, Ninth Revision, Clinical Modification code...
March 2, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28329303/high-burden-of-antimicrobial-resistance-and-mortality-among-adults-and-children-with-community-onset-bacterial-infections-in-india
#12
Vidya Mave, Ajay Chandanwale, Anju Kagal, Sandhya Khadse, Dileep Kadam, Renu Bharadwaj, Vaishali Dohe, Matthew Robinson, Aarti Kinikar, Samir Joshi, Priyanka Raichur, Katie McIntire, Savita Kanade, Jonathan Sachs, Chhaya Valvi, Usha Balasubramanian, Vandana Kulkarni, Aaron M Milstone, Ivan Marbaniang, Jonathan Zenilman, Amita Gupta
Background.: In India, antimicrobial consumption is high, yet systematically collected data on the epidemiology, risk factors and outcomes of antimicrobial resistant (AMR) infections are limited. Methods.: A prospective study of adults and children hospitalized for acute febrile illness (AFI) was conducted between August 2013 and December 2015. In-hospital outcomes were recorded, and logistic regression was performed to identify independent predictors of AMR community-onset infections...
March 3, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28329274/trichinella-nativa-outbreak-with-rare-thrombotic-complications-associated-with-meat-from-a-black-bear-hunted-in-northern-ontario
#13
Daniel Dalcin, Dante S Zarlenga, Nicholas C Larter, Eric Hoberg, Daniel A Boucher, Samuel Merrifield, Rachel Lau, Filip Ralevski, Karamjit Cheema, Kevin L Schwartz, Andrea K Boggild
Background: Although trichinellosis is known to cause thrombotic disease, serious thrombotic events are rare and have not been previously associated with Trichinella nativa infection. Methods: Patient interviews and medical chart reviews were conducted on 10 men who became ill following consumption of a common source of black bear meat. Trichinella serology on patient sera as well as PCR and larval identification of the meat samples was conducted. Results: All 10 exposed individuals developed an acute illness clinically compatible with trichinellosis, characterized by fever, abdominal pain, and diarrhea, along with eosinophilia ranging from 0...
February 22, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28329259/evaluation-of-modified-2-tiered-serodiagnostic-testing-algorithms-for-early-lyme-disease
#14
John A Branda, Klemen Strle, Lise E Nigrovic, Paul M Lantos, Timothy J Lepore, Nitin S Damle, Mary Jane Ferraro, Allen C Steere
Background.: The conventional 2-tiered serologic testing protocol for Lyme disease (LD), an enzyme immunoassay (EIA) followed by immunoglobulin M and immunoglobulin G Western blots, performs well in late-stage LD but is insensitive in patients with erythema migrans (EM), the most common manifestation of the illness. Western blots are also complex, difficult to interpret, and relatively expensive. In an effort to improve test performance and simplify testing in early LD, we evaluated several modified 2-tiered testing (MTTT) protocols, which use 2 assays designed as first-tier tests sequentially, without the need of Western blots...
January 27, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28329238/practice-patterns-and-outcomes-associated-with-procalcitonin-use-in-critically-ill-patients-with-sepsis
#15
David C Chu, Anuj B Mehta, Allan J Walkey
Background: Randomized trials support use of procalcitonin-based algorithms to decrease duration of antibiotics for critically ill patients with sepsis. However, current utilization of procalcitonin and associated outcomes in "real world" clinical settings is unclear. We sought to determine procalcitonin utilization in critically ill patients with sepsis in the United States and to examine associations between procalcitonin use and clinical outcomes. Methods: Retrospective cohort study of approximately 20% of patients hospitalized in US Intensive Care Units with sepsis...
February 24, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28329167/repeated-vaccination-does-not-appear-to-impact-on-influenza-vaccine-effectiveness-against-hospitalisation-with-confirmed-influenza
#16
Allen C Cheng, Kristine K Macartney, Grant W Waterer, Tom Kotsimbos, Paul M Kelly, Christopher C Blyth
Background: Annual influenza vaccine is recommended for those at greatest risk of severe influenza infection. Recent reports of a negative impact of serial influenza vaccination on vaccine effectiveness raises concerns about the recommendation for annual influenza vaccines, particularly in those at greatest risk. Methods: The Influenza Complications Alert Network (FluCAN) is an Australian hospital-based sentinel surveillance program. In this observational study, cases were defined as subjects >9 years admitted with influenza confirmed by PCR...
March 17, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28329019/a-cross-sectional-analysis-of-traditional-medicine-use-for-malaria-alongside-free-antimalarial-drugs-treatment-amongst-adults-in-high-risk-malaria-endemic-provinces-of-indonesia
#17
Dwi Linna Suswardany, David W Sibbritt, Sudibyo Supardi, Jerico F Pardosi, Sungwon Chang, Jon Adams
BACKGROUND: The level of traditional medicine use, particularly Jamu use, in Indonesia is substantial. Indonesians do not always seek timely treatment for malaria and may seek self-medication via traditional medicine. This paper reports findings from the first focused analyses of traditional medicine use for malaria in Indonesia and the first such analyses worldwide to draw upon a large sample of respondents across high-risk malaria endemic areas. METHODS: A sub-study of the Indonesia Basic Health Research/Riskesdas Study 2010 focused on 12,226 adults aged 15 years and above residing in high-risk malaria-endemic provinces...
2017: PloS One
https://www.readbyqxmd.com/read/28328974/retinal-oximetry-measures-systemic-hypoxia-in-central-nervous-system-vessels-in-chronic-obstructive-pulmonary-disease
#18
Thorunn Scheving Eliasdottir, David Bragason, Sveinn Hakon Hardarson, Charles Vacchiano, Thorarinn Gislason, Jona Valgerdur Kristjansdottir, Gudrun Kristjansdottir, Einar Stefánsson
BACKGROUND: Determination of the blood oxyhemoglobin saturation in the retinal vessels of the eye can be achieved through spectrophotometric retinal oximetry which provides access to the state of oxyhemoglobin saturation in the central nervous system circulation. The purpose of this study was to test the capability of the Oxymap T1 oximeter to detect systemic hypoxemia and the effect of supplemental oxygen on retinal vessel oxyhemoglobin saturation. METHODS: Oxygen saturation of hemoglobin in retinal arterioles and venules was measured in 11 subjects with severe chronic obstructive pulmonary disease (COPD) on long term oxygen therapy...
2017: PloS One
https://www.readbyqxmd.com/read/28328972/altered-gut-microbiome-in-a-mouse-model-of-gulf-war-illness-causes-neuroinflammation-and-intestinal-injury-via-leaky-gut-and-tlr4-activation
#19
Firas Alhasson, Suvarthi Das, Ratanesh Seth, Diptadip Dattaroy, Varun Chandrashekaran, Caitlin N Ryan, Luisa S Chan, Traci Testerman, James Burch, Lorne J Hofseth, Ronnie Horner, Mitzi Nagarkatti, Prakash Nagarkatti, Stephen M Lasley, Saurabh Chatterjee
Many of the symptoms of Gulf War Illness (GWI) that include neurological abnormalities, neuroinflammation, chronic fatigue and gastrointestinal disturbances have been traced to Gulf War chemical exposure. Though the association and subsequent evidences are strong, the mechanisms that connect exposure to intestinal and neurological abnormalities remain unclear. Using an established rodent model of Gulf War Illness, we show that chemical exposure caused significant dysbiosis in the gut that included increased abundance of phylum Firmicutes and Tenericutes, and decreased abundance of Bacteroidetes...
2017: PloS One
https://www.readbyqxmd.com/read/28328964/the-acute-mania-of-king-george-iii-a-computational-linguistic-analysis
#20
Vassiliki Rentoumi, Timothy Peters, Jonathan Conlin, Peter Garrard
We used a computational linguistic approach, exploiting machine learning techniques, to examine the letters written by King George III during mentally healthy and apparently mentally ill periods of his life. The aims of the study were: first, to establish the existence of alterations in the King's written language at the onset of his first manic episode; and secondly to identify salient sources of variation contributing to the changes. Effects on language were sought in two control conditions (politically stressful vs...
2017: PloS One
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