Joycelyn Tan, Sam Virtue, Dougall M Norris, Olivia J Conway, Ming Yang, Guillaume Bidault, Christopher Gribben, Fatima Lugtu, Ioannis Kamzolas, James R Krycer, Richard J Mills, Lu Liang, Conceição Pereira, Martin Dale, Amber S Shun-Shion, Harry Jm Baird, James A Horscroft, Alice P Sowton, Marcella Ma, Stefania Carobbio, Evangelia Petsalaki, Andrew J Murray, David C Gershlick, James A Nathan, James E Hudson, Ludovic Vallier, Kelsey H Fisher-Wellman, Christian Frezza, Antonio Vidal-Puig, Daniel J Fazakerley
The in vitro oxygen microenvironment profoundly affects the capacity of cell cultures to model physiological and pathophysiological states. Cell culture is often considered to be hyperoxic, but pericellular oxygen levels, which are affected by oxygen diffusivity and consumption, are rarely reported. Here, we provide evidence that several cell types in culture actually experience local hypoxia, with important implications for cell metabolism and function. We focused initially on adipocytes, as adipose tissue hypoxia is frequently observed in obesity and precedes diminished adipocyte function...
April 5, 2024: EMBO Journal